 and more. Alex then creates his teas and herbal infusions from teas he has sourced from estates around the world. Its as simple as that. The tea then arrives in a personalised tin with the recipe on it and how it reflects the receiver.
What you might call a personalised gift with a very personal touch and ideal as a Christmas gift.To be placed on one of our “waiting list(s)” we require that families first complete our Puppy Questionnaire, which is found on our questionnaire section of our website, then if approved they pay their nonrefundable deposit of $400.00. This goes towards the overall price of one of our adorable pups and shows us that the family is committed. Families, on our waiting list(s), have up until the time the puppies are three weeks of age to give us their final decision. After this, we’ll be opening up the “availability” of the remaining gorgeous pups, to others who successfully pass our interviewing process. Final payment of the families’ chosen pup(s) is due upon pick up, if not having delivered by our Puppy Nanny, and we only accept cash or PayPal.
NEET cable keepers are bendable zip-on sleeves for phone chargers, headphones, and other cables. (Because, let’s face it, anything with a cable probably has a tangled cable.) More proof that inspiration can come from anywhere: this line was inspired by a trip to the jewelers. Peter Chin, a manufacturing pro, was buying a necklace for his wife. He noticed how the clerk wrapped it to make sure the chain stayed tangle free. Realizing all the other things he could untangle, Pete went to work and, less than week later, NEET was born. Thanks to the bendable wire frame beneath its fabric, after you zip in your cables, you can fold or shape them and they’ll stay that way. Use NEET to store two or more cables as needed…good news for those of you listening to music on your smart phone. And, with multiple, bold colors, cables stay organized and easy to identify. No more wondering whose earbuds are whose.Hydrogels Containing Antibiofilm and Antimicrobial Agents Beneficial for Biofilm-Associated Wound Infection: Formulation Characterizations and In vitro Study
Abstract
Bacterial biofilm which adheres onto wound surface is shown to be impervious to antibiotics and this in turn delays wound healing. Previous studies showed that antibiofilm agents such as xylitol and ethylenediaminetetraacetic acid (EDTA) prevent bacterial adherence onto surfaces. Formulation of a wound dressing containing antibiofilm agents may be a plausible strategy in breaking the biofilm on wound surfaces and at the same time increase the efficacy of the antibiotic. The purpose of this study was to develop hydrogel formulations containing antibiofilm agents along with antibiotic (gentamicin) for bacterial biofilm-associated wound infection. Sodium carboxymethyl cellulose (NaCMC) hydrogels loaded with antibiofilm agents and antibiotic were prepared. The hydrogels were characterized for their physical properties, rheology, Fourier transform infrared spectroscopy (FTIR), drug content uniformity, differential scanning calorimetry (DSC) and in vitro drug release study. The antibiofilm (Crystal Violet staining and XTT assay) and antibacterial performances of the hydrogels against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli were assessed in vitro. The formulated hydrogels showed adequate release of both antibiofilm agents (xylitol and EDTA). Both antimicrobial and antibiofilm tests showed promising results and demonstrated that the combination of xylitol, EDTA, and gentamicin had an additive effect against both Gram-positive and Gram-negative bacteria. In summary, NaCMC (sodium carboxymethyl cellulose) hydrogels containing the combination of antimicrobial and antibiofilm agents were successfully developed and this can be a new strategy in combating biofilm in wound infection which in turn accelerate wound healing.
KEY WORDShydrogel antibiofilm agent xylitol EDTA biofilm
Notes
Acknowledgements
The authors would like to thank the Ministry of Science, Technology and Innovation Malaysia, MOSTI (Grant No. 02-01-02-SF1228) and the Faculty of Pharmacy, UKM, for their research facility support.
Compliance with Ethical Standards
Conflict of Interest
The authors report that they have no conflicts of interest.
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