RESULTS	At baseline and 3 hours after the injection , we assessed ( 1 ) coronary flow reserve , aortic distensibility , systolic and diastolic ( Em ) velocity of the mitral annulus , and E to Em ratio ( E/Em ) using echocardiography ; ( 2 ) flow-mediated , endothelium-dependent dilation of the brachial artery ; and ( 3 ) malondialdehyde , nitrotyrosine , interleukin-6 , endothelin-1 , and C-reactive protein .\