METHODS	The exercise protocol was carried out on a treadmill set at a 3 % uphill grade and consisted of walking at 2 m/sec for 2 min , trotting for 10 min at 3.7 m/sec , galloping for 2 min at 14 m/sec ( which elicited maximal heart rate ) , trotting for 20 min at 3.7 m/sec , walking for 10 min at 1.8 m/sec , cantering for 8 min at 9.2 m/sec , trotting for 1 min at 5 m/sec and walking for 5 min at 2 m/sec .\