METHODS	Study 2 was a secondary analysis of a double-blind randomized controlled study conducted between August 19 , 2005 , and August 24 , 2010 , that included 25 individuals with untreated diabetes or impaired fasting glucose who were randomized to receive placebo ( n = 13 ) or a combination of metformin , 1000 mg twice daily , and pioglitazone , 45 mg daily ( n = 12 ) , for 3 months .\