text: Yo, check it out, listen up, here's the scoop,
  We used the TM1 domain, ain't no loop,
  Of the large conductance mechanosensitive (MS) channel,
  From Escherichia coli, you know we handle.
  
  We took that genetic probe, went on a search,
  In the archaeon Methanoccoccus jannashii, it's a lurch,
  Looking for some MscL homologs, that's right,
  And MJ0170 is what came into sight.
  
  M. jannashii's hypothetical protein, you see,
  Showed 38.5% sequence identity to Eco-MscL, guarantee,
  And MJ0170, it's a homolog of MscS, don't you know,
  E. coli's second type of MS channel, yggB, let's go!
  
  Hold up, there's more, let me drop some knowledge,
  In the C-terminus of MJ0170, there's a college,
  A cluster of charged residues, KIKEE, a real treasure,
  That looks a lot like Eco-MscL's RKKEE, for real, no pressure.
  
  We ain't playing around, we took it to the next phase,
  Cloned and expressed MJ0170, set it ablaze,
  In E. coli, liposomes, giant spheroplasts, no doubt,
  It showed activity like the bacterial MS channels, shout it out!
  
  Our study shows the evolution, the grand plan,
  M. jannashii's MS channel and its homologs, understand,
  They came from gene duplication, no illusion,
  With the TM1 domain, the most conserved motif, that's the conclusion.