text: Yo, check it out, listen up, I got a tale to tell,
  'Bout the separation of enantiomers, molecules that dwell,
  Important in biology, chiral pharmaceuticals, amino acids,
  Their activity differs, yo, that's what the fact is.
  
  We gotta separate 'em, that's the mission we pursue,
  Direct methods or indirect, that's the route we choose,
  Derivatization with chiral reagents, that's the game,
  Labeling 'em amino groups, carboxyl groups, all the same.
  
  Yo, we got methodologies, HPLC's the key,
  Diastereomer formation, that's how we set 'em free,
  Using chiral benzofurazan-bearing reagents, we roll,
  DBD-APy and DBD-PyNCS, let me show you how they go.
  
  4-(3-aminopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole,
  That's the first one, DBD-APy, let the story unfold,
  And 4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole,
  DBD-PyNCS, yo, that's the second, now you know.
  
  These reagents, they derivatize, separate, detect,
  Chiral molecules in the mix, they can't neglect,
  So if you're into chiral separations, peep this chapter right here,
  Learn the methods, get the knowledge, let it all be clear.