text: Yo, check it out, let me break it down for you
  Talkin' 'bout that hydroperoxide of linoleic acid, 13-HPODE
  It degrades into 9,12-dioxo-10(E)-dodecenoic acid, DODE, you know what I'm sayin'?
  And that DODE, it be gettin' all up in them proteins, modifiyin' 'em
  
  This study, it went and found them major proteins in MCF7 cells
  All modified by that DODE, straight up, nothin' else
  To keep it real and reduce them false positives, they used three methods
  Listen up, I'ma break it down, so you don't forget it
  
  First, they treated them cells with that 13-HPODE-biotin
  Synthetically biotinylated, gettin' them modified proteins
  Enriched by neutravidin affinity, then identified
  Two-dimensional liquid chromatography--tandem mass spectrometry, yeah, that's how they ride
  
  Second method, they went and treated them cells with native 13-HPODE
  Protein carbonyls gettin' biotinylated, you know how it go
  Then enriched them modified proteins with neutravidin affinity
  Identified 'em again with 2D LC-MS/MS, that's the key
  
  Last but not least, they came through with that DODE antibody
  Newly developed, locatin' them DODE-modified proteins, can't you see?
  2D sodium dodecyl sulfate--polyacrylamide gel electrophoresis and Western blot
  In-gel digestion and LC-MS/MS, yeah, they hit the spot
  
  When they analyzed them proteins usin' all three methods
  They found a significant overlap, no need for skeptics
  Identified 32 primary proteins modified by DODE in them MCF7 cells
  Showin' the possibility for future indicators of oxidative stress, oh hell yeah, it tells
  
  So there you have it, the science behind it all
  DODE protein-carbonyl adducts, ready to make the call
  This study showed the feasibility, the potential, and the way
  To understand cellular formation of DODE, keepin' it real, day by day