text: Yo, check it out, let me break it down
  Alternative splicing and alternative polyadenylation, hold your crown
  These processes bring diversity to the transcriptome
  Coding capacity, gene regulation, they're never alone
  
  We used second-gen sequencing to analyze the scene
  But short-read data had a limitation, it wasn't keen
  Predicting full-length splice isoforms was a tough task
  So we switched it up, took a different path
  
  We sequenced the sorghum transcriptome, no time to waste
  With Pacific Biosciences, we went for the long-read chase
  Introducing TAPIS, our pipeline's name
  Transcriptome Analysis Pipeline for Isoform Sequencing, it's the game
  
  We identified full-length splice isoforms, no doubt
  11,000 of 'em, we brought 'em out
  But wait, there's more, we got surprises in store
  APA of around 11,000 expressed genes, we explore
  
  Over 2,100 novel genes, they're on the rise
  Sorghum gene annotations, we amplify
  This bioenergy crop, we're studying its gene regulation
  Enhancing our knowledge, it's the ultimate destination
  
  TAPIS is the tool, the one you need
  To analyze Iso-Seq data, it's a guaranteed feed
  No matter the organism, it's got you covered
  Unlocking the secrets, no longer smothered