text: Yo, peep the methods for atomic force microscopy (AFM),
  Imaging human metaphase chromosomes, it's a gem.
  Lymphocyte chromosomes fixed and spread on slides of glass,
  Observed in phosphate-buffered saline, that's top-class.
  
  But hold up, we switchin' gears to BALL-1 cell line,
  Isolating chromosomes with hexylene glycol, so fine.
  Absorbed on silane-coated glass, we got the setup right,
  In hexylene glycol buffer, we observe 'em in sight.
  
  AFM droppin' three-dimensional topographic view,
  Fixed or unfixed chromosomes, height info comin' through.
  We even snagged the ultrastructural image of chromatids,
  AFM in liquid condition, no need to hide.
  
  Now let me tell you 'bout the combo, AFM and light,
  Cytochemically and immunocytochemically stained, that's tight.
  Breakin' it down, studyin' chemical components' place,
  In relation to chromosome structure, it's a steady chase.