text: Yo, check it out, I got a new proposal,
  A silver-enhanced colloidal gold metalloimmunoassay, yo,
  Determinin' Schistosoma japonicum antibody (SjAb) in rabbit serum,
  Listen close, 'cause this is how it's gonna occur.
  
  First, the adult worm antigen of S. japonicum (SjAg),
  Gets adsorbed passively, stickin' to the microwell's walls, straight up,
  Then it reacts with the desired SjAb, no doubt,
  Colloidal gold-labeled goat anti-rabbit IgG secondary antibody's what we're 'bout.
  
  It gets adsorbed on the microwell's walls, you see,
  Through the reaction with SjAb, that's the key,
  Silver enhancement process comes next, listen clear,
  Dissolving silver metal atoms, acidic solution's here.
  
  Determination of dissolved silver ions, that's the plan,
  Using anodic stripping voltammetry (ASV), understand?
  At a glassy-carbon electrode, we're gettin' it done,
  Optimizing the conditions, makin' sure it's a home run.
  
  Reaction time of SjAg with SjAb, gotta be right,
  Interaction of SjAb with the secondary antibody, tight,
  Dilution ratio of the secondary antibody, crucial too,
  Silver enhancement time, we gotta nail that too.
  
  Now, the anodic stripping peak current tells us all,
  It depends linearly on SjAb concentration, stand tall,
  Range is from 6.4 ng/ml to 100 microg/ml,
  Ain't nothin' better, this assay's got the skill.
  
  Detection limit's low, only 3.0 ng/ml of SjAb,
  Beats the piezoelectric wave sensor, don't you grab,
  Their detection limit's 7.2 microg/ml, that's weak,
  And the renewable amperometric immunosensor's detection limit's 0.36 microg/ml, take a seat.
  
  We analyzed rabbit serum with different infection degrees,
  Results showin' the method's legit, can't you see?
  Meets the requirements of clinical analysis, no jest,
  This silver-enhanced colloidal gold assay's the best.