text: Yo, check it out, let me break it down for you,
  Talkin' 'bout complex I, it's a puzzle, it's true.
  Its structure is complex, ain't nothin' trivial,
  With numerous protein players, it's critical.
  
  In bacterial complex I, we got fourteen subunits,
  Seven hydrophilic in the peripheral arm, no disputin'.
  The other seven hydrophobic, in the membrane arm,
  Identifyin' those proteins brings a whole lotta harm.
  
  Complex I from Rhodothermus marinus, we investigated,
  With an original approach, the game was elevated.
  Protein separation strategies, we had a few,
  Reversed phase materials, liquid chromatography too.
  
  We used gel electrophoresis, they all played a part,
  Identification methods, we brought 'em to the chart.
  Electrospray ionization, tandem mass spectrometry,
  Matrix-assisted laser desorption, that's the key.
  
  We even used Edman degradation analysis,
  To unravel the mysteries, no need for paralysis.
  We made progress in characterizin' membrane proteins,
  But let me tell you, it's still a challenge, no illusions.
  
  With these methods combined, we made the breakthrough,
  Identified canonical subunits, and somethin' new.
  A possible novel subunit, a PCD we found,
  Pterin-4α-carbinolamine dehydratase, holdin' it down.
  
  It's the first time a PCD's linked to complex I,
  Its regulatory role, let's take it to the sky.
  We discussed its potential, its impact on the game,
  Complex I's got secrets, we're still in search of fame.