text: AIM, check it out, we wanna know
  If LLO can make that DNA flow
  Incorporation, pH dependent toxin
  Listeriolysin O, it's rockin'
  
  METHODS in play, we got a plan
  A multi-component system, understand?
  ASG, PL, and LLO combined
  To enhance DNA escape, we're gonna find
  
  Marker genes, luciferase and beta galactosidase
  Plasmids in the mix, let's set the stage
  Administered in vitro to Huh7 and SK Hep1
  ASG receptor positive and negative, we test the fun
  
  Purity, activity, expression, and more
  We evaluated it all, hardcore
  RESULTS came in, listen up, y'all
  LLO conjugate had that targetin' call
  
  Specificity and membranolytic might
  ASG receptor cells, the expression took flight
  Luciferase gene, more than 7-fold increase
  No significant expression in receptor negative peace
  
  Beta galactosidase, a smaller boost
  But still increased significantly, that's the truth
  Toxicity, we found none, at effective doses
  Transfection studies showed no composure losses
  
  CONCLUSIONS are clear, let me break it down
  ASOR-PL coupled with LLO, we found
  Targeting and increased gene expression, yo
  Foreign DNA's got a new flow