text: Yo, listen up, I got a study to share,
  Comparin' MRSA isolates, yeah, we're aware.
  We used PFGE and bacteriophage type,
  To analyze these bugs, man, it was hype.
  
  PCR and RFLP, we took it to the lab,
  To get some subtyping info, ain't no time to gab.
  PFGE done with kits and software,
  Standardized and commercial, we were prepared.
  
  Coa and spa genes, we checked 'em too,
  Used PCR to amplify, that's what we do.
  Coa hypervariable and spa repeat regions,
  Polymorphisms, man, we got all the reasons.
  
  RFLPs with HaeIII and AluI,
  Produced 38 patterns, yeah, that's true.
  Identified the epidemic types, 15 and 16,
  EMRSA on the scene, causing a scene.
  
  EMRSA 15, it had a unique site,
  RFLP and sequence analysis, man, it was tight.
  Coa gene got the spotlight, no doubt,
  EMRSA 16, it didn't have that clout.
  
  Spa gene PCR gave us profiles galore,
  14 in total, man, we were ready for more.
  Repeats ranged from 3 to 18, that's the range,
  EMRSA types, they were in their own lane.
  
  Coa and spa polymorphisms, they're the key,
  For strain identification, man, can't you see?
  Discriminatory subtyping, that's what we gained,
  Surveillance of MRSA, we're on top, unrestrained.