text: Caulobacter crescentus, CB15,
  Replication initiation, let me break it down, see,
  Stalked cells, they're the ones that start the show,
  Swarmers, nah, they don't get to go.
  
  Dimorphic control, that's what we seek,
  Freshwater Caulobacter and the marine physique,
  Isolated replication origins, you know the drill,
  From FWC and MCS, we're keeping it real.
  
  IHF and CcrM, they play a role,
  DNA methylation sites, they got control,
  But the ori IHF and CcrM we found,
  Only conserved in closely related FWCs, that's profound.
  
  DnaA boxes and CtrA, they're in the mix,
  CB15 ori components, that's the tricks,
  CtrA, a regulator with two components,
  Blocks replication in swarmers, that's the correspondents.
  
  DnaA boxes and CtrA sites, we found 'em right,
  In five FWCs and three MCS oris, out of sight,
  Usually paired together, that's the game,
  CtrA binding regulates DnaA's flame.
  
  We put it to the test, site-directed mutagenesis,
  MCS10 ori, we made some changes,
  One CtrA binding site, it's crucial, you see,
  Overlapping a DnaA box, unique in the whole genome spree.
  
  Selective mutations in the DnaA box, replication decreased,
  Selective mutations in the CtrA site, replication increased,
  So both FWC and MCS, they're in cahoots,
  Using CtrA to repress replication pursuits.
  
  But hold up, wait, there's more in this tale,
  Phylogenetic analysis, it's about to unveil,
  CtrA usage, evolved separately, yeah, it's true,
  Among these Caulobacter oris, a surprising view.
  
  Consensus oris and convergent ori evolution,
  In differentiating bacteria, a revolution,
  That's the story, the facts, the whole dish,
  Caulobacter knowledge, served with a rap twist.