goal to determine the exact number of template in the sample
{"Image DocumentName": "Goal: to determine the exact number of template in the sample", "Image ImageDescription": "Absolute quantification. Ct. Goal: to determine the exact number of template in the sample. -synthesis of a external reference = - target synthesized in vitro. -determine the exact quantity of synthetized molecules (by spectrophotometry) -do serial dilutions of the reference. -(RT)-PCR on the serial dilution of the reference. -draw a standard curve for the reference with the exact quantity on the X axis and the Ct on the Y axis. Ct. -report the Ct obtained for the sample on the standard curve. exact quantity.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}