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[Agency] NIH [Project Title] COMPARATIVE BIOINFORMATICS AND TP53 EXPRESSION [Institution] PURDUE UNIVERSITY WEST LAFAYETTE [Amount] $69,066 [Summary] This project's aims are as follows: (1) Very dense comparative alignment of the TP53/GBN5 cis-regulatory region across mammals; (2) Characterization of transcriptional responses using cell lines from a wide variety of species; (3) Molecular footprinting of this promoter region across mammals; (4) Test hypotheses of regulatory evolution using deletion/site-directed mutation constructs with reporter gene assays. As with (2) a key novel aspect of this work is the use of wildtype fibroblast cell lines from many different orders of mammals; (5) Using a novel pair-wise cell line assay, separate cis (local) from trans (non-local) effects in the evolution of transcriptional regulation; (6) Use, test, and develop bioinformatic/genomic and molecular evolutionary techniques on the dense sequence alignment of (1), plus genomes of over 14 species of mammals. [Completion/ Closing Date] 08/20/2011 [Link] link [Category] 
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