f8c80f4d_ics_in_Mammalian______Protocol__Action
[0] 3 [Purpose and Goal] This temporary increase in pressure should clear any obstruction at the tip. After releasing the plunger, it should rise on its own accord; if it does not, this means pressure is not being built-up in the syringe and you need to tighten the connections and/or add additional vacuum grease. [Action] 
Depress the plunger to the very end of the syringe
Analysis of mRNA Nuclear Export Kinetics in Mammalian... | Protocol
Action