04d0c4d7__characterisation_of_risk_loci__0
[1] Transient or stable transfection mediated by plasmid or lentiviral expression vectors. [2] Cell lines: Modulation of expression levels achieved often using constructs with strong promoters (e.g. CMV) but tissue-specific and inducible promoters can also be used to modulate gene expression. [3] Fast, easy, economical and reproducible. However, it is often difficult to obtain an expression comparable to physiologic levels or in a cell cycle-specific pattern. There may be artifactual effects resulting from over-expression. [4] Analysis should be performed in pools as well as in multiple clonal isolates to control for clonal variation or adaptation. References and resources: [168] [0] 
Ectopic gene over-expression
WikiGenes - Principles for the post-GWAS functional characterisation of risk loci
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