b16d28c9__ChIP_Help___Novus_Biologicals__Issue
[Recommendations] Verify that your antibody of interest is ChIP validated. Specificity of antibody can be verified by western blot after IP. Too little antibody can result in too little material for successful PCR. Too much can increase PCR background. Some antibodies may allow short room temperature incubations with lysate but in general an overnight incubation at 4° C will increase signal and specificity. [Issue] 
Antibody
Chromatin Immunoprecipitation Troubleshooting: ChIP Help | Novus Biologicals
Issue