04d0c4d7__characterisation_of_risk_loci__0
[1] DNA-binding nucleases that can be used for genomic editing. It creates DSBs at specified sites which are normally repaired by error-prone NHEJ. A transfected template including a mutation favors homology-directed recombination leading to a knock-in of the desired mutation. [2] Cell lines: Manipulation of cell’s genotype by introduction/changes in specific SNPs into cell lines (e.g. myelogenous leukaemia K562 cell line). [3] Same as described for cell lines (see above) [4] The generation of a DSB leads to a high frequency of modified clones. Challenges include limited availability of off-the-shelf cell lines and very high cost. References and resources: [162][163] [0] 
Gene targeting by zinc finger nucleases
WikiGenes - Principles for the post-GWAS functional characterisation of risk loci
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