517d7ea4_to_Neurons___Current_Protocols__Problem
[Possible cause] Clogging of the glass micropipet during virus injection [Solution] During the injection procedure verify repeatedly that virus solution exits from the pipet tip by lifting the micropipet out of the cutting medium and applying pressure from the syringe [Problem] 
Limited infection of slice cultures
Alphaviruses: Semliki Forest Virus and Sindbis Virus Vectors for Gene Transfer into Neurons - Current Protocols
Problem