81a56b55_Biosafety__otes___Specific_Considerations
[NIH Subcategory] D-3 Experiments involving the use of infectious DNA or RNA viruses or defective DNA or RNA viruses in the presence of a helper virus in tissue culture systems.
Experiments involving Risk Group 2 viruses (infectious or defective) in the presence of helper virus are generally conducted at BL2) Experiments involving Risk Group 3 viruses (infectious or defective) in the presence of helper virus are generally conducted at BL3. Recombinant DNA or RNA molecules containing < 2/3 of the genome of any eukaryotic virus are considered defective. These viruses, in the absence of a helper virus, are categorized under III-E.
Biosafety
Notes & Specific Considerations