517d7ea4_to_Neurons___Current_Protocols__Problem
[Possible cause] Suboptimal electroporation conditions or excessive DMRIE‐C reagent [Solution] Optimize transfection conditions [Problem] 
Poor cell viability following cotransfection of RNA
Alphaviruses: Semliki Forest Virus and Sindbis Virus Vectors for Gene Transfer into Neurons - Current Protocols
Problem