517d7ea4_to_Neurons___Current_Protocols__Problem
[Possible cause] Did not use an optimal concentration of infectious replicons [Solution] Construct a dose‐response curve with the infectious replicons to determine the amount required for optimal expression [Problem] 
Low recombinant protein expression in infected cells
Alphaviruses: Semliki Forest Virus and Sindbis Virus Vectors for Gene Transfer into Neurons - Current Protocols
Problem