f8c80f4d_ics_in_Mammalian______Protocol__Action
[0] 6 [Purpose and Goal] This further agitates the particulate within the needle and helps reposition it to relieve the obstruction at the tip. Stronger scratching may chip off the end of the tip, enabling the obstruction to exit the needle. [Action] 
Scratch the needle tip on a clean portion of the coverslip
Analysis of mRNA Nuclear Export Kinetics in Mammalian... | Protocol
Action