ee9c752d_Flow_Cytometry_Facility__Use
[Fixation Method] Detergent Lysis [Reagents] Triton X-100 Tween 80 NP 40 Saponin [Advantages] Rapid Requires fewer washes [Problems] Cell fragility Loss of cytoplasmic cell components High debris component Limited storage time [Use] 
Rapid permeabilization Native conformation of intracellular antigens Requires fewer washes, preserving limited cell numbers
Flow Cytometry Facility
Use