517d7ea4_to_Neurons___Current_Protocols__Problem
[Possible cause] Excessive pressure used for virus injection [Solution] Use micropipets with a smaller tip opening; apply less pressure [Problem] 
Slice cultures show many dead, non‐infected cells around injection sites
Alphaviruses: Semliki Forest Virus and Sindbis Virus Vectors for Gene Transfer into Neurons - Current Protocols
Problem