04d0c4d7__characterisation_of_risk_loci__0
[1] Modification of cell’s or organism’s genotype to assess allele-specific effects using targeted recombination vectors [2] Cell lines: Manipulation of cell’s genotype by introduction/changes in specific SNPs into cell lines (e.g. colorectal cancer HCT116 cell line). [3] Cell lines can be used for biochemistry studies, drug response screens, short and long term cell biological assays (e.g. apoptosis, survival fraction assays, soft agar growth assays). Limitations include the inability to assess effects from the in vivo milieu or developmentally-restricted effects. [4] Relatively low frequency of integration necessitating the screening of a large number of clones. Efficiency of integration may be gene specific as it depends on the structure of the homology fragments flanking the target locus. While a targeting vector with a G418r (or Hygr) cassette driven by the phosphoglycerate kinase promoter can be effectively used to target regulatory regions, it is expected to increase the number of random events References and resources: [153][154][155][156][157] _ENREF_2[158][159][160][161] [0] 
Homologous recombination
WikiGenes - Principles for the post-GWAS functional characterisation of risk loci
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