Traceback (most recent call last):


 "/Users/yanghui/PycharmProjects/quantest/my_strategy/复盘/notices.py", line 23, in <module>

if key_word in notices.title:


 "/Library/Frameworks/Python.framework/Versions/3.5/lib/python3.5/site-packages/pandas/core/generic.py", line 871, in __contains__

return key in self._info_axis


 "/Library/Frameworks/Python.framework/Versions/3.5/lib/python3.5/site-packages/pandas/indexes/base.py", line 1393, in __contains__

hash(key)


 "/Library/Frameworks/Python.framework/Versions/3.5/lib/python3.5/site-packages/pandas/core/generic.py", line 831, in __hash__

' hashed'.format(self.__class__.__name__))
TypeError: 'DataFrame' objects are mutable, thus they cannot be hashed

Traceback (most recent call last):

 "/group/ngs/src/bcbio-nextgen/0.8.1a/rhel6-x64/anaconda/lib/python2.7/site-packages/bcbio/distributed/ipythontasks.py", line 38, in _setup_logging
yield config

 "/group/ngs/src/bcbio-nextgen/0.8.1a/rhel6-x64/anaconda/lib/python2.7/site-packages/bcbio/distributed/ipythontasks.py", line 79, in process_alignment
return ipython.zip_args(apply(sample.process_alignment, *args))

 "/group/ngs/src/bcbio-nextgen/0.8.1a/rhel6-x64/anaconda/lib/python2.7/site-packages/bcbio/pipeline/sample.py", line 114, in process_alignment
data)

 "/group/ngs/src/bcbio-nextgen/0.8.1a/rhel6-x64/anaconda/lib/python2.7/site-packages/bcbio/pipeline/cleanbam.py", line 30, in picard_prep
return _filter_bad_reads(rg_bam, ref_file, data)

 "/group/ngs/src/bcbio-nextgen/0.8.1a/rhel6-x64/anaconda/lib/python2.7/site-packages/bcbio/pipeline/cleanbam.py", line 51, in _filter_bad_reads
do.run(cmd, "Filter problem reads")

 "/group/ngs/src/bcbio-nextgen/0.8.1a/rhel6-x64/anaconda/lib/python2.7/site-packages/bcbio/provenance/do.py", line 22, in run
_do_run(cmd, checks, log_stdout)

 "/group/ngs/src/bcbio-nextgen/0.8.1a/rhel6-x64/anaconda/lib/python2.7/site-packages/bcbio/provenance/do.py", line 121, in _do_run
raise subprocess.CalledProcessError(exitcode, error_msg)CalledProcessError: Command '/group/ngs/src/bcbio-nextgen/0.8.1a/rhel6-x64/bin/gatk-framework -Xms2000m -Xmx5500m -XX:+UseSerialGC -Djava.io.tmpdir=/ngs/scratch/bcbiotmp/tmp5xA31j -U LENIENT_VCF_PROCESSING --read_filter BadCigar --read_filter NotPrimaryAlignment -T PrintReads -R /ngs/reference_data/genomes/Hsapiens/hg19/seq/hg19.fa -I /gpfs/ngs/oncology/Analysis/external/EXT_018_SRP042620/variant/work/bamclean/SRR1313121/SRR1313121-reorder-fixrgs.bam --out /gpfs/ngs/oncology/Analysis/external/EXT_018_SRP042620/variant/work/bamclean/SRR1313121/tx/tmpS4dmrL/SRR1313121-reorder-fixrgs-gatkfilter.bam --filter_mismatching_base_and_quals --filter_bases_not_stored --filter_reads_with_N_cigar