Traceback (most recent call last):

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/kombu/connection.py", line 436, in _ensured
return fun(*args, **kwargs)

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/kombu/messaging.py", line 177, in _publish
channel = self.channel

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/kombu/messaging.py", line 194, in _get_channel
channel = self._channel = channel()

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/kombu/utils/__init__.py", line 425, in __call__
value = self.__value__ = self.__contract__()

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/kombu/messaging.py", line 209, in <lambda>
channel = ChannelPromise(lambda: connection.default_channel)

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/kombu/connection.py", line 757, in default_channel
self.connection

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/kombu/connection.py", line 742, in connection
self._connection = self._establish_connection()

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/kombu/connection.py", line 697, in _establish_connection
conn = self.transport.establish_connection()

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/kombu/transport/pyamqp.py", line 116, in establish_connection
conn = self.Connection(**opts)

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/amqp/connection.py", line 165, in __init__
self.transport = self.Transport(host, connect_timeout, ssl)

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/amqp/connection.py", line 186, in Transport
return create_transport(host, connect_timeout, ssl)

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/amqp/transport.py", line 299, in create_transport
return TCPTransport(host, connect_timeout)

 "/Users/sumit.goel/.virtualenvs/myfocus/lib/python3.5/site-packages/amqp/transport.py", line 95, in __init__
raise socket.error(last_err)OSError: [Errno 61] Connection refused
Traceback (most recent call last):


 "/shared/pipeline-user/tools/local/bin/bcbio_nextgen.py", line 4, in <module>

__import__('pkg_resources').run_script('bcbio-nextgen==1.2.3', 'bcbio_nextgen.py')


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/pkg_resources/__init__.py", line 665, in run_script

self.require(requires)[0].run_script(script_name, ns)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/pkg_resources/__init__.py", line 1463, in run_script

exec(code, namespace, namespace)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/bcbio_nextgen-1.2.3-py3.6.egg/EGG-INFO/scripts/bcbio_nextgen.py", line 245, in <module>

main(**kwargs)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/bcbio_nextgen-1.2.3-py3.6.egg/EGG-INFO/scripts/bcbio_nextgen.py", line 46, in main

run_main(**kwargs)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/bcbio_nextgen-1.2.3-py3.6.egg/bcbio/pipeline/main.py", line 58, in run_main

fc_dir, run_info_yaml)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/bcbio_nextgen-1.2.3-py3.6.egg/bcbio/pipeline/main.py", line 91, in _run_toplevel

for xs in pipeline(config, run_info_yaml, parallel, dirs, samples):


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/bcbio_nextgen-1.2.3-py3.6.egg/bcbio/pipeline/main.py", line 154, in variant2pipeline

samples = genotype.parallel_variantcall_region(samples, run_parallel)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/bcbio_nextgen-1.2.3-py3.6.egg/bcbio/variation/genotype.py", line 208, in parallel_variantcall_region

"vrn_file", ["region", "sam_ref", "config"]))


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/bcbio_nextgen-1.2.3-py3.6.egg/bcbio/distributed/split.py", line 35, in grouped_parallel_split_combine

final_output = parallel_fn(parallel_name, split_args)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/bcbio_nextgen-1.2.3-py3.6.egg/bcbio/distributed/ipython.py", line 137, in run

for data in view.map_sync(fn, items, track=False):


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/client/view.py", line 344, in map_sync

return self.map(f,*sequences,**kwargs)


 "<decorator-gen-140>", line 2, in map


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/client/view.py", line 52, in sync_results

ret = f(self, *args, **kwargs)


 "<decorator-gen-139>", line 2, in map


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/client/view.py", line 37, in save_ids

ret = f(self, *args, **kwargs)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/client/view.py", line 1114, in map

return pf.map(*sequences)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/client/remotefunction.py", line 299, in map

return self(*sequences, __ipp_mapping=True)


 "<decorator-gen-122>", line 2, in __call__


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/client/remotefunction.py", line 80, in sync_view_results

return f(self, *args, **kwargs)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/client/remotefunction.py", line 285, in __call__

return r.get()


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/client/asyncresult.py", line 169, in get

raise self.exception()


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/client/asyncresult.py", line 228, in _resolve_result

results = error.collect_exceptions(results, self._fname)


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/error.py", line 233, in collect_exceptions

raise e


 "/shared/pipeline-user/bcbio/anaconda/lib/python3.6/site-packages/ipyparallel/error.py", line 231, in collect_exceptions

raise CompositeError(msg, elist)
ipyparallel.error.CompositeError: one or more exceptions from call to method: variantcall_sample
