Traceback (most recent call last):


 "/home/aadam/bcbio/tools/bin/bcbio_nextgen.py", line 234, in <module>

main(**kwargs)


 "/home/aadam/bcbio/tools/bin/bcbio_nextgen.py", line 43, in main

run_main(**kwargs)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/pipeline/main.py", line 42, in run_main

fc_dir, run_info_yaml)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/pipeline/main.py", line 86, in _run_toplevel

for xs in pipeline(config, run_info_yaml, parallel, dirs, samples):


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/pipeline/main.py", line 258, in rnaseqpipeline

samples = qcsummary.generate_parallel(samples, run_parallel)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/pipeline/qcsummary.py", line 35, in generate_parallel

qced = run_parallel("pipeline_summary", to_analyze)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/distributed/multi.py", line 28, in run_parallel

return run_multicore(fn, items, config, parallel=parallel)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/distributed/multi.py", line 86, in run_multicore

for data in joblib.Parallel(parallel["num_jobs"], batch_size=1)(joblib.delayed(fn)(x) for x in items):


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/joblib/parallel.py", line 804, in __call__

while self.dispatch_one_batch(iterator):


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/joblib/parallel.py", line 662, in dispatch_one_batch

self._dispatch(tasks)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/joblib/parallel.py", line 570, in _dispatch

job = ImmediateComputeBatch(batch)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/joblib/parallel.py", line 183, in __init__

self.results = batch()


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/joblib/parallel.py", line 72, in __call__

return [func(*args, **kwargs) for func, args, kwargs in self.items]


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/utils.py", line 50, in wrapper

return apply(f, *args, **kwargs)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/distributed/multitasks.py", line 186, in pipeline_summary

return qcsummary.pipeline_summary(*args)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/pipeline/qcsummary.py", line 68, in pipeline_summary

data["summary"] = _run_qc_tools(work_bam, work_data)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/pipeline/qcsummary.py", line 144, in _run_qc_tools

out = qc_fn(bam_file, data, cur_qc_dir)


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/qc/qualimap.py", line 329, in run_rnaseq

metrics.update(_detect_rRNA(data))


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/site-packages/bcbio/qc/qualimap.py", line 257, in _detect_rRNA

quant = os.path.join(sailfish_dir, "quant", "quant.sf")


 "/home/aadam/bcbio/genomes/anaconda/lib/python2.7/posixpath.py", line 70, in join

elif path == '' or path.endswith('/'):
AttributeError: 'NoneType' object has no attribute 'endswith'
Traceback (most recent call last):


 "C:\Python27\lib\site-packages\kivy\core\window\window_sdl2.py", line 619, in mainloop

self._mainloop()


 "C:\Python27\lib\site-packages\kivy\core\window\window_sdl2.py", line 362, in _mainloop

EventLoop.idle()


 "C:\Python27\lib\site-packages\kivy\base.py", line 330, in idle

self.dispatch_input()


 "C:\Python27\lib\site-packages\kivy\base.py", line 304, in dispatch_input

provider.update(dispatch_fn=self._dispatch_input)


 "C:\Python27\lib\site-packages\kivy\input\providers\wm_pen.py", line 143, in update

self.pen.update_time_end()
AttributeError: 'NoneType' object has no attribute 'update_time_end'
