In silico AFLP-PCR amplification
In silico AFLP-PCR amplification In silico AFLP-PCR amplification Start using this tool Introduction Digestion and ligation to adaptors PCR amplification with adaptor specific primers Resulting band pattern Suggestion of restriction enzymes pairs for AFLP-PCR Introduction Amplified Fragment Length Polymorphism PCR (AFLP-PCR) was originally described by Zabeau & Vos in 1993 . The procedure of this technique is divided into three steps: Digestion of total cellular DNA with one or more restriction enzymes and ligation of restriction half-site specific adaptors to all restriction fragments.