ISTI – Effective 1 January 2023  Page 85 of 92 J.4 Requirements for Dried Blood Spot Sample Collection Equipment  The dried blood spot Sample  Collection Equipment shall fulfill the following criteria:  a) Contain a single -use Sample collection device ( e.g., disposable lancets to be used in conjunction with cellulose cards, devices with integrated microneedle(s)/microlancet(s)) for the puncture/incision and collection of capillary blood at the fingertip and/or from the upper arm (alternative sites of punctures may be authorized for Athletes  with physical impairments, if required);  b) The “A” and “B” absorbent Sample support shall allow the collection of distinct “A” and “B” spots (or equivalent) with a minimum  total of approximately 40 µL of capillary blood in t he “A” spot(s) and with a minimum  total of approximately 20 µL of capillary blood in the “B” spot(s)  and; [Comment to J.4  (b): Depending on the dried blood spot Sample Collection Equipment  used, the volume and number of spots may vary.