When using the CUSUM, results should be assessed using customary procedures as detailed at http://itl.nist.gov/div898/handbook/pmc/section3/pmc323.htm   6.0 Analytical Testing Procedure and Reporting of Test Results  6.1 Initial Quantification of the Markers  − Two (2)  Aliquots  taken from the original “A” Sample  shall be analyzed once (x1) to quantify intact IGF -I and P -III-NP;       ABP Operating Guidelines  – Version 9.0 – July 2023  Page 36/91 − QC Sample(s), at low-  and high- levels of the Marker s (see Article 5.3), shall be included in each i nitial quantification analytical batch;  − The coefficient of variation (CV%) between the duplicate determinations of the IGF -I and P -III-NP concentrations shall not be higher (≤) than the associated uc_Max (see Table 1) .