UC San scientists and MIT and Harvard ’s Broad Institute have created a new method to study gene networks through screening complicated genetic interactions and phenotypes in single cells . The “ Perturb - seq ” system , which uses single - cell RNA sequencing , will aid scientists in understanding how healthy gene networks can go wrong in response to environmental stimuli such as CRISPR - based perturbations . The system has already been used to make new observations on dendritic cell immune response , mapping how genes interact with one another through the measurement of phenotypic changes . It is hoped the sequencing will be more powerful in preparation for further in - depth gene mapping .
UC San Francisco and MIT and Harvard’s Broad Institute have created a new technique to map gene networks through screening complicated genetic interactions and phenotypes in single cells. The “Perturb-seq” system, which uses single-cell RNA sequencing, will aid scientists in understanding how healthy gene networks can go wrong in response to environmental stimuli such as CRISPR-based perturbations. The system has already been used to make new observations on dendritic cell immune response, mapping how genes interact with one another through the measurement of phenotypic changes. It is hoped the sequencing will become more powerful in future for further in-depth gene study.