( CNN ) -- World heavyweight champion Wladimir Klitschko knocked out U.S. challenger Eddie Chambers with five seconds remaining in the 12th and final round to defend his IBF , WBO and IBO titles . The Saturday bout in Duesseldorf , Germany , was the 33-year-old Ukrainian 's fifth defense of his WBO and IBF titles . Chambers went down for at least two minutes after receiving Klitschko 's left hook to the right temple , according to CNNSI 's Bryan Armen Graham round-by-round blog of the fight . Read Graham 's account of the fight . Early in the fight Chambers -- at 209 pounds ( 94.8 kg ) , 36 pounds ( 16.3 kg ) lighter than Klitschko 's 245 ( 111.1 kg ) -- lifted his opponent into the air twice , dumping him onto the canvas . Chambers had managed to outmaneuver Klitschko 's punches until the end despite being outmatched . Chambers won some extra recovery time after getting his broken right glove replaced in the 10th round . The 8-to-1 favorite Klitschko 's win is his 12th straight successful title defense . He has has n't lost a fight in nearly six years . His brother Vitali is the WBC heavyweight champion . CNNSI 's Bryan Armen Graham contributed to this report .
Chambers goes down for five seconds after left hook to right temple . Chambers , at 94.8 kg ( 94.8 kg ) is 16.3 kg ( 16.3 kg ) lighter than Eddie Chambers . Klitschko was an 8-to-1 favorite . He has n't lost a fight in Saturday .