In the mid-1980s, following concerns about HIV, sperm which had been frozen and thawed by BPAS was used instead of fresh sperm.
ɪnðə mˈɪd nˈaɪntiːnhˈʌndɹɪd ˈeɪɾi z, fˈɑːloʊɪŋ kənsˈɜːnz ɐbˌaʊt ˌeɪtʃˌaɪvˈiː, spˈɜːm wˌɪtʃ hɐdbɪn fɹˈoʊzən ænd θˈɔːd baɪ bˈiːpˈæs wʌz jˈuːzd ɪnstˈɛd ʌv fɹˈɛʃ spˈɜːm.