The sequence of human GM-CSF was first identified in 1985 and soon three recominbant human GM-CSFs were produced, one in bacteria, one in mammalian cells, and one in yeast; Immunex developed GM-CSF manufactured in yeast into Leukine.
ðə sˈiːkwəns ʌv hjˈuːmən dʒˌiːˈɛmsˌiːˌɛsˈɛf wʌz fˈɜːst aɪdˈɛntᵻfˌaɪd ɪn nˈaɪntiːnhˈʌndɹɪd ˈeɪɾi fˈaɪv ænd sˈuːn θɹˈiː ɹˌiːkˈɑːmɪnbənt hjˈuːmən dʒˌiːˈɛmsˌiːˌɛsˈɛfs wɜː pɹədˈuːst, wˈʌn ɪn bæktˈɪɹiə, wˈʌn ɪn mæmˈeɪliən sˈɛlz, ænd wˈʌn ɪn jˈiːst; ɪmˈʌnɛks dɪvˈɛləpt dʒˌiːˈɛmsˌiːˌɛsˈɛf mˌænjuːfˈæktʃɚd ɪn jˈiːst ˌɪntʊ lˈuːkaɪn.