ðə spˈɪɹətʃˌiːt loʊkˈeɪʃən ɪz aɪdˈɛntᵻfˌaɪd jˈuːzɪŋ ðə fˈɪtk stˈeɪnɪŋ, ænd ðə tɹˈɪtk stˈeɪnɪŋ aɪdˈɛntᵻfˌaɪz wˈɛðɚ ðə pˈeɪʃənt hɐz ˈæntaɪtˈiː. pælˈɪdəm ˈæntɪbˌɑːdiz bˈaɪndɪŋ tə ðə sˈeɪm spˈɪɹətʃˌiːt.
The spirochete location is identified using the FITC staining, and the TRITC staining identifies whether the patient has anti-T. pallidum antibodies (binding to the same spirochete).