fˈɪtk ɐ flˈʊɹɹəfˌoːɹlˈeɪbəld ˈæntaɪtɹˈɛpoʊnˌiːm ˈæntɪbˌɑːdi ænd tɹˈɪtk ɐnˈʌðɚ flˈʊɹɹəfˌoːɹlˈeɪbəld ˈæntaɪhjˈuːmən ˈæntɪbˌɑːdiz ɑːɹ ˈædᵻd æz sˈɛkəndɚɹi ˈæntɪbˌɑːdiz.
FITC (a fluorophore)-labeled anti-treponeme antibody and TRITC (another fluorophore)-labeled anti-human antibodies are added as secondary antibodies.