ðɪs sˈɪŋɡəl ɐmˈiːnoʊ ˈæsɪd tʃˈeɪndʒ ɪnðɪ ˌɛnˈɛs wˈʌn dʒˈiːn ɡɹˈeɪtli ɪŋkɹˈiːst ðə pˌæθoʊdʒˌɛnˈɪsᵻɾi ʌvðɪ ˈeɪtʃ fˈaɪv ˈɛn wˈʌn ˌɪnfluːˈɛnzə vˈaɪɹəs.
This single amino acid change in the NS1 gene greatly increased the pathogenicity of the H5N1 influenza virus.