ɪŋkɹˈiːsɪŋ ðə jˈiːld ʌv səksᵻnˈeɪt fɹʌm mˈɪkst ˈæsɪd fɜːməntˈeɪʃən wʌz fˈɜːst dˈʌn baɪ ˌoʊvɚɹɛkspɹˈɛsɪŋ ðɪ ˈɛnzaɪm pˌiːˌiːpˈiː kˈɑːɹbəksˌɪleɪz.
Increasing the yield of succinate from mixed acid fermentation was first done by overexpressing the enzyme PEP carboxylase.