ðə mˈoʊst kˈɑːmən sˈʌbstɹeɪt fɔːɹ dʒˌiːjˌuːˈɛs hˌɪstəkˈɛmɪkəl stˈeɪnɪŋ ɪz fˈaɪvbɹˈoʊmoʊ fˈoːɹklˌoːɹoʊ θɹˈiːˈɪndəlˌɪl ɡlˈuːkjʊɹɹˌɑːnaɪd ˈɛksɡlˈʌk.
The most common substrate for GUS histochemical staining is 5-bromo-4-chloro-3-indolyl glucuronide (X-Gluc).