ɪn vˈɪtɹoʊ tˈɪʃuː ɡɹˈæftɪŋ ɛkspˈɛɹɪmənts ʃˈoʊ ðæt ɹᵻmˈuːvəl ʌv ðɪs mˈɑːlɪkjˌuːl pɹɪvˈɛnts dˌɪfɚɹˌɛnʃɪˈeɪʃən ʌvðə flˈoːɹ plˈeɪt, wˈɛɹæz ɪts ɛktˈɑːpɪk ɛkspɹˈɛʃən ɪndˈuːsᵻz dˌɪfɚɹˌɛnʃɪˈeɪʃən ʌv flˈoːɹ plˈeɪt sˈɛlz.
In vitro tissue grafting experiments show that removal of this molecule prevents differentiation of the floor plate, whereas its ectopic expression induces differentiation of floor plate cells.