ˌɛnˈɛs wˈʌn mˌaɪt ˈɔːlsoʊ ɪnhˈɪbɪt splˈaɪsɪŋ ʌv pɹˈiːˈɛm ˌɑːɹɹˌɛnˈeɪ baɪ bˈaɪndɪŋ tʊ ɐ stˈɛmbˈʌldʒ ɹˈiːdʒən ɪn jˈuː sˈɪks smˈɔːl nˈuːklɪɹ ˌɑːɹɹˌɛnˈeɪ ˌɛsˈɛn ˌɑːɹɹˌɛnˈeɪ.
NS1 might also inhibit splicing of pre-mRNA by binding to a stem-bulge region in U6 small nuclear RNA (snRNA).