ɪt klˈiːvz ɐ fˈɑːsfoʊdɪstɚ bˈɑːnd fˈoːɹ nˈuːklɪˌɑːɾaɪdz dˈaʊnstɹiːm ʌvðə dˌiːˌɛnˈeɪ dˈæmɪdʒ, ænd klˈiːvz ɐ fˈɑːsfoʊdɪstɚ bˈɑːnd ˈeɪt nˈuːklɪˌɑːɾaɪdz ʌpstɹˈiːm ʌvðə dˌiːˌɛnˈeɪ dˈæmɪdʒ ænd kɹiːˈeɪts ɐ twˈɛlv nˈuːklɪˌɑːɾaɪd ɛksˈaɪzd sˈɛɡmənt.
It cleaves a phosphodiester bond four nucleotides downstream of the DNA damage, and cleaves a phosphodiester bond eight nucleotides upstream of the DNA damage and creates a twelve nucleotide excised segment.