ˈɛvənz blˈuː dˈaɪ hˈæzbiːn jˈuːzd æz ɐ vˌaɪəbˈɪlᵻɾi ɐsˈeɪ ɔnðə bˈeɪsɪs ʌv ɪts pˌɛnɪtɹˈeɪʃən ˌɪntʊ nˈɑːnvˈaɪəbəl sˈɛlz, ɔːlðˈoʊ ðə mˈɛθəd ɪz sˈʌbdʒɛkt tʊ ˈɛɹɚ bɪkˈʌz ɪɾ ɐsˈuːmz ðæt dˈæmɪdʒd ɔːɹ ˈʌðɚwˌaɪz ˈɔltɚd sˈɛlz ɑːɹ nˌɑːt kˈeɪpəbəl ʌv ɹᵻpˈɛɹ ænd ðˈɛɹfoːɹ ɑːɹ nˌɑːt vˈaɪəbəl.
Evans blue dye has been used as a viability assay on the basis of its penetration into non-viable cells, although the method is subject to error because it assumes that damaged or otherwise altered cells are not capable of repair and therefore are not viable.