mˈɪlsbɚɹˌoʊ ɪz loʊkˈeɪɾᵻd ɪn fˈɑːɹ sˈʌðɚn wˈɑːʃɪŋtən kˈaʊnti, ɐlˈɔŋ pˌɛnsɪlvˈeɪniə ɹˈaʊt ˈeɪɾi ˈeɪt, wˌɪtʃ tɹˈævəlz ɐlˈɔŋ ðə mˌɑːnoʊŋɡɐhˈɛlɚ ɹˈɪvɚ.
Millsboro is located in far southern Washington County, along Pennsylvania Route 88, which travels along the Monongahela River.