ðə mˈeɪdʒɚ lˈɪmɪɾɪŋ fˈæktɚ fɔːɹ ðɪs ˌɛndʒɪnˈɪɹɪŋ ɪz ðə nˈiːd tə meɪntˈeɪn ɐ ɹᵻdˈɑːks bˈæləns ɪnðə mˈɪkstʃɚɹ ʌv ˈæsɪdz pɹədˈuːst baɪ ðə fɜːməntˈeɪʃən pˈæθweɪ.
The major limiting factor for this engineering is the need to maintain a redox balance in the mixture of acids produced by the fermentation pathway.