ɪn hjˈuːmənz sˈʌtʃ ɹɪˈækʃən ɪz pɚfˈɔːɹmd baɪ baɪfˈʌŋkʃənəl θɹˈiːfˈɑːsfoʊdˌɛnəsˌaɪn fˈaɪvfˈɑːsfəsˌʌlfeɪt sˈɪnθeɪsᵻz pˈæps wˈʌn ænd pˈæps tˈuː jˈuːzɪŋ ˌeɪtˌiːpˈiː æz ðə fˈɑːsfeɪt dˈoʊnɚ.
In humans such reaction is performed by bifunctional 3′-phosphoadenosine 5′-phosphosulfate synthases (PAPSS1 and PAPSS2) using ATP as the phosphate donor.