ðɪs ɪz ɐkˈɑːmplɪʃt θɹuː fˈɑːsfoʊpptˌaɪd mˈæpɪŋ, wˌɪtʃ ɪnvˈɑːlvz daɪdʒˈɛstʃən əvɚ ɹˌeɪdɪoʊˈæktɪvli lˈeɪbəld pɹˈoʊtiːn, sˌɛpɚɹˈeɪʃən ʌv fˈɑːsfoʊpptˌaɪd pɹˈɑːdʌkts, ænd fˈaɪnəli ɐnˈæləsˌɪs vˈaɪə hˈaɪpɚfˈoːɹməns lˈɪkwɪd kɹoʊmɐtˈɑːɡɹəfi ˌeɪtʃpˌiːˌɛlsˈiː ɔːɹ mˈæs spɛktɹˈɑːmətɹi.
This is accomplished through phosphopeptide mapping, which involves digestion of a radioactively labeled protein, separation of phosphopeptide products, and finally analysis via high-performance liquid chromatography (HPLC) or mass spectrometry.