fɹʌm wˈʌn θˈaʊzənd fˈaɪvhˈʌndɹɪd kˌɑːmpətˈɪʃən fɹʌm ˈʌðɚ pˈɑːɹts ʌv jˈʊɹɹəp, ɪspˈɛʃəli ˈɪŋɡlənd, ɡɹˈuː ænd lˈeɪdən lˈɔst ɪts lˈiːdɪŋ ɹˈoʊl.
From 1500 competition from other parts of Europe, especially England, grew and Leiden lost its leading role.