ɐdˈɪʃənəli, ðɪ ɪntˌɜːpɹɪtˈeɪʃən ʌvðɪ ɐsˈeɪ ɪz lˈɪmɪɾᵻd baɪ ðə mˈuːvmənt ʌv di ˈɛksˈɪndɪɡˌoʊ θɹuːˈaʊt ðə sˈɛl.
Additionally, the interpretation of the assay is limited by the movement of diX-indigo throughout the cell.