In Eastleigh's earlier Wessex League days they had rivalries with Sholing Sports, Hamble as well as a continued rivalry with A.F.C. Totton, who beat them 2-1 in the Wessex League cup final in the 2002-2003 season.
As Eastleigh joined the Conference it lost any league games to teams within a radius of 40 miles, but regularly contested the Hampshire Derby with Aldershot Town.