In the movie Beavis and Butthead Do America, the original Treasure Island sign, along with the pirate ship outside the hotel, are briefly seen as Beavis and Butthead are driving down the Las Vegas strip in a limo.
In the movie Knocked Up, Ben (Seth Rogen) and Pete (Paul Rudd) see Mystère by Cirque du Soleil at Treasure Island during their road trip to Las Vegas.