Beginning at the I-95 interchange in southeastern Duval as the West Beltway, the beltway travels west, passes through the Mandarin area with interchanges at Old St. Augustine Road, and SR 13 (San Jose Boulevard).
East of the I-95 interchange near the airport, I-295 continues east as the East Beltway, with the next interchange being US 17. I-295 starts to curve to the southeast, with interchanges at Pulaski Road, and Alta Drive/Yellowbluff Road.