Mice with induced sickle cell disease and their controls exhibited a "pain face" when tested on a cold plate, but sickle mice showed increased intensity compared to controls; this was confirmed using Von Frey filaments a traditional method of pain assessment [REF].
It has been noted that DBA/2 strain mice, but not CBA strain mice, show an increase in GS score following only isoflurane anaesthesia, which should be taken into account when using the GS to assess pain.