Botrydial originates from the BcBOT2 (Botrytiscinerea BOTrydial biosynthesis) mediated cyclization of farnesyl diphosphate (FPP) to key intermdiate tricyclic alcohol presilphiperfolan-8β-ol. Pinedo et al. demonstrated that BcBOT2 is in fact a sesquiterpene synthase by incubation of FPP with recombinant BcBOT2 protein, which yielded the expected presilphiperfolan-8-ol as the major product.
4 other genes are then involved in the biosynthesis of botrydial: 3 genes that encode for a P450 monooxygenase (BcBOT3, BcBOT1,BcBOT4) and a BcBOT5 gene whose amino acid sequence showed high homology to known acetyl transferases.