He was the eldest son of Diego Hurtado de Mendoza y de Luna, 3rd Duke of the Infantado (1461-1531)[REF][REF][REF][REF][REF] and María Pimentel[REF][REF][REF][REF][REF] a daughter of the 4th Count and 1st Duke of Benavente, Rodrigo Alonso Pimentel and María Pacheco Portacarerro, hence also known as María Pimentel y Pacheco [REF].
He had a brother known as Rodrigo de Mendoza, 1st Marquess of Montesclaros (or Montes-Claros) and a sister described as Ana de Mendoza, who married Luis de La Cerda, 1st Marquess of Cogolludo [REF].