Barbara was the youngest child of Jerzy Radziwiłł, Voivode of Trakai and Vilnius and Great Lithuanian Hetman, and his wife Barbara Kolanka, daughter of Voivode of Podolia [REF].
In 1536, Stanislovas Goštautas, Voivode of Nowogrodek, canceled his betrothal to Anna Elżbieta Radziwiłł, elder sister of Barbara [REF].