In Windows 3.x and 95/98/ME, the boot loader phase is handled by MS-DOS.
When all system configuration files and device drivers have been loaded, the 16-bit modules, KRNL386.EXE, GDI.EXE, and USER.EXE, are loaded, then the 32-bit DLLs (KERNEL32.DLL, GDI32.DLL, and USER32.DLL) are loaded.