Composer Scott Bradley quoted it in his MGM cartoon scores of the 1940s, including the Oscar-winning Tom and Jerry short "Yankee Doodle Mouse" (1943) and Tex Avery's "King-Size Canary" (1947).
In the 1966 Howard Hawks western El Dorado, the character Bull, in response to being shot at from a bell-laden church tower and then asked to provide cover, proclaims, "Well, just give me another gun and I'll play "Marching Through Georgia." .