In 1538, Jacqueline married, as his first wife, Louis III de Bourbon-Montpensier, who would become Duke of Montpensier on the death of his mother Louise de Bourbon, Duchess of Montpensier on 5 July 1561.
François de Bourbon, Duke of Montpensier (1542- 4 June 1592), married in 1566, Renée d'Anjou, Marquise de Mézières (21 October 1550- 1597), daughter of Nicolas d'Anjou, Marquis de Mézières and Gabrielle de Mareuil, by whom he had one son, Henri de Bourbon, Duke of Montpensier.