Several mechanisms have been proposed for the production of hemozoin in Plasmodium, and the area is highly controversial, with membrane lipids,[REF][REF] histidine-rich proteins,[REF] or even a combination of the two,[REF] being proposed to catalyse the formation of hemozoin.
Although β-hematin can be produced in assays spontaneously at low pH, the development of a simple and reliable method to measure the production of hemozoin has been difficult.