The most important feature of phosphoramidites is their ability to undergo the phosphoramidite coupling reaction that is, to react with nucleophilic groups in the presence of an acidic azole catalyst, 1H-tetrazole, 2-ethylthiotetrazole,[REF] 2-benzylthiotetrazole,[REF][REF] 4,5-dicyano imidazole,[REF] or a number of similar compounds.
Phosphoramidites are readily oxidized with weak oxidating reagents, for instance, with aqueous iodine in the presence of weak bases or with hydrogen peroxide[REF] to form the respective phosphoramidates.