Based on this, it is often assumed that a file on an ISO 9660 formatted disc cannot be larger than 232-1 in size, as the file's size is stored in an unsigned 32 bit value, for which 232-1 is the maximum.
It is, however, possible to circumvent this limitation by using the multi-extent (fragmentation) feature of ISO 9660 Level 3 to create ISO 9660 filesystems and single files up to 8 TB.