The next Bohemian king was Ferdinand Habsburg, husband of Ann Jagellon, who was the sister of Ludwig Jagellon.
Next in line for Bohemian crown was Rudolf's brother Matthias, but since Matthias was childless, his cousin, the archduke Ferdinand of Styria (related also to Jagellon, Luxemburg and Premyslovec Dynasties), was initially accepted by the Bohemian Diet as heir presumptive when Matthias became ill.