The unit saw action in the sieges of Petersburg and Richmond where it was involved in the Battle of the Crater (July 30, 1864), Battle of Globe Tavern (August 18-21, 1864), Battle of Poplar Grove Church (September 29-October 1, 1864), and Battle of Boydton Plank Road (October 27-28, 1864).
The 29th Regiment Infantry, United States Colored Troops was mustered out on November 6, 1865.