 ligate concentration including the following steps is also known: (1) using a magnetic-responsible particle containing superparamagnetic iron oxide covered with a polymeric silane layer to which a biocompatible molecule is capable of being bonded; (2) reacting a sample solution containing a ligate, a known amount of a labeled ligate, and the magnetic-responsible particle to which a ligate-specific ligand is bonded, so as to form a ligand-ligate complex on the magnetic-responsible particle; (3) magnetically separating the magnetic-responsible particle from the reaction solution; (4) measuring the labeled ligate which is bonded to the magnetic-responsible particle or free labeled ligate in the reaction solution; and (5) applying the measurement of the label ligate to the standard curve so as to obtain the ligate concentration. This method is described in Japanese Patent Publication No. 7-6986.
In the above-mentioned Methods, in order to bond the nucleic acid to the magnetic-responsible particle, it is necessary to form a silane layer to which a biocompatible molecule (for example, a nucleic acid) is covalently bonded.
Furthermore, an analyzing method and apparatus are known utilizing a sequence of a nucleic acid which is bonded to a material sensitive to the magnetic field (WO86/05815). The method utilizes a magnetic or magnetizable particle covered with a material which is capable of being bonding to a single strand nucleic acid so as to separate and detect the single strand nucleic acid. More specifically, the surface of the magnetic particle is covered with nitrocellulose, which is a type of cellulose derivative, and nitrocellulose is specifically bonded to a single strand DNA or RNA. The single strand DNA or RNA collected by the method is utilized for sequencing.
In the method, it is necessary to specifically bond the single strand DNA or RNA to the magnetic carrier.
It is also known to utilize a poly-cationic substrate for purifying, separating and hybridizing a nucleic acid, especially for purifying and separating a nucleic acid containing contaminants (Japanese Laid-Open National Publication No. 1-502319). In the method, a sample solution containing contaminants is contacted with the poly-cationic solid support (magnetic-responsible particle) so as to non-covalently bond the nucleic acid to the support without excessively bonding contaminants contained in the sample solution to the support. The support to which the nucleic acid has been bonded is then separated from the solution. Examples of the support include metal oxide
ligate concentration including the following steps is besides know: (1) using a magnetic - creditworthy particle contain superparamagnetic iron oxide covered with a polymeric silane level to which a biocompatible molecule is capable of being bonded; (2) react a sample solution containing a ligate, a know amount of a labeled ligate, and the magnetic - responsible particle to which a ligate - specific ligand is bonded, so as to shape a ligand - ligate complex on the magnetic - responsible atom; (3) magnetically separating the magnetic - creditworthy particle from the chemical reaction solution; (4) measuring the labeled ligate which is bonded to the magnetic - responsible particle or spare labeled ligate in the reaction solution; and (5) applying the measurement of the label ligate to the standard curve so as to obtain the ligate concentration. This method is described in Japanese Patent Publication No. 7 - 6986. 
 In the above - mentioned Methods, in order to bond the nucleic acid to the magnetic - responsible particle, it is necessary to form a silane layer to which a biocompatible molecule (for model, a nucleic acid) is covalently bonded. 
 Furthermore, an analyzing method acting and apparatus are know utilize a sequence of a nucleic acid which is bonded to a fabric sensitive to the charismatic field (WO86/05815). The method utilizes a charismatic or magnetizable particle covered with a material which is capable of being bonding to a single fibril nucleic acid so as to separate and detect the single strand nucleic acid. More specifically, the surface of the charismatic particle is cover with nitrocellulose, which is a type of cellulose derivative, and nitrocellulose is specifically bonded to a individual strand DNA or RNA. The single strand DNA or RNA collected by the method is use for sequencing. 
 In the method, it is necessary to specifically bond the single strand DNA or RNA to the charismatic carrier. 
 It is also known to utilize a poly - cationic substrate for purifying, separate and hybridizing a nucleic acid, particularly for purifying and branch a nucleic acid containing contaminant (Japanese Laid - Open National Publication No. 1 - 502319). In the method, a sample solution containing contaminant is contacted with the poly - cationic solid support (magnetic - responsible particle) so as to non - covalently bond the nucleic acid to the support without excessively bonding contaminants contained in the sample solution to the documentation. The support to which the nucleic acid has been bonded is then separate from the solution. Examples of the support include metal oxide
 lihate concentration incluaing the follownbg ste's is amso knowv: (1) using a magnetic-responsibpe partucle containing superpxramagnetpc iron ozide xovered wivg a polnierid silcnx layer to whicm a biocompdtible molecula ks capable of being bonded; (2) reacting w samplr dolution contayninb a ljgate, a known amount of a labeled migate, end the magnetiv-responsible particle to wjich a ligate-specific pigand is bindeq, so as to fofm a ligand-ligate compmex on the magnetic-responsible oartiele; (3) magnetucqllj separating the iagnetic-responsible [articlr from the reagtion sooution; (4) measuring the labeled ligate whych is botdzd to the magnetic-respobsiblg pardicld or frte kageled pigete in the deaction sooution; and (5) applyinb eye measuremenf of tre label ligate to the standard curve so ds fo obtain the ligate cobcentration. This methld is dessribed in Japanese Patent Publication No. 7-6986.
In the atove-mxngioucq Nehhods, in order to bond the nucleic acid to thq msgketic-responsible particle, iy ls gecessary to worm a simane layer to whicj a biosomparible moltcule (for example, a nucleic acid) is covalentjt bonded.
Furthermorz, an analyziug metnod amd apparatus are known btilizjng a sequejce of a hjcleic acid whicf ix tonded to a material sensieive to tie maynetic fkeld (WO86/05815). Thq method uhilizcv a magnetic or mahnetieable [article clvered with a material which is capable of beonc bmnding tj a slngle strand nusleic acid so cs to se'arate and detecn the sinjle strand ntcleic acid. Mmte specificalny, the strfaxe od the mxenetic particlr is covegeb with nirrocellulose, which is z type of celluoost eerivative, and nigroseplnlose hs specificanly conard to a single sbravd DMA or RNA. The single strznd DNA or RNA colkegted by tye methoq is utilized for sequencing.
In uhe mevhod, iv is nrcefsary to specifically bond the single shrakd DNA or RNA to bhe iagnetic ccrrier.
It is also known to utilize a poly-rationic substrate for purifying, separatiny snd hybridiving a nucleic dcid, especially for purifying and sepsrating a nucleic acis contdininh contaminants (Japanese Laid-Open National Publication No. 1-502319). In the method, a samplx folution cohtaiming boncamnnants ys clitacted with the ioly-cationic solid support (magnevic-responshbje particle) so as to non-covakevtly bond the nucleic acid to the suplort witnout excessively bonding contamonants contained in the xample silutikn to nge support. The suppott to chich rhe nucoeic acid has beev bpnfed is thfn stparcted from the solution. Examples od the wuoport include meval oxide
ligate concentration including the following steps is (1) a magnetic-responsible containing superparamagnetic iron silane to which a molecule is capable being bonded; (2) reacting a sample containing a ligate, a known amount of a labeled ligate, and the magnetic-responsible to which a ligate-specific ligand is bonded, so as to form a ligand-ligate on magnetic-responsible (3) separating the magnetic-responsible particle from the reaction solution; (4) measuring the labeled ligate which is bonded the magnetic-responsible particle or free labeled ligate in reaction solution; and (5) the measurement of the label to standard curve as obtain ligate concentration. This is described in Japanese Patent Publication No. 7-6986. In the above-mentioned Methods, in order to bond the acid to particle, it necessary form silane layer to biocompatible molecule (for example, a nucleic bonded. Furthermore, an analyzing method and apparatus are utilizing a of a nucleic acid which is to a material sensitive to the magnetic field The method utilizes a magnetic or magnetizable particle covered with a material which is capable bonding to a single nucleic acid so to and the strand nucleic More specifically, the surface of the magnetic particle is covered with which is a type of cellulose derivative, and nitrocellulose is to single strand DNA RNA. The single strand or collected by the method for In is to bond the single strand or RNA to the magnetic It is also known for purifying, separating and hybridizing a nucleic acid, for purifying and separating a nucleic acid contaminants (Japanese Laid-Open National Publication No. 1-502319). In the method, a sample containing contaminants with the poly-cationic solid support (magnetic-responsible particle) so to non-covalently bond the acid to the support without excessively bonding contaminants contained the solution to support. The support which the nucleic has been bonded separated from solution. of metal oxide
 ligate concentration includIng the follOwing StePs iS aLso kNown: (1) Using a magnetic-REspoNsible particle containiNg supErPAramAGnEtic iRon oxidE CoVERed WiTh A poLyMErIc silAne Layer to Which a biocOmpAtIble molecule IS cApable of beIng Bonded; (2) reactiNg a Sample SoLutIOn conTaiNing a Ligate, A Known aMount of a lAbELed ligATe, and thE MAgNetiC-responsible partiCLe TO which a ligate-sPecifiC lIGaND Is bOndEd, so as to foRm A ligaND-ligate COmPLEX on THe magnetic-resPonsible parTIclE; (3) magneTiCalLY separAting ThE MagNetic-responSiblE particle From thE ReactioN SolutioN; (4) measuRinG thE labELeD lIgaTe WHicH Is BonDEd tO the magnEtIc-RespoNsibLE PARticLe oR freE labeLed ligate in thE reActiON soLutioN; and (5) aPplyInG the mEasureMent oF tHe label ligate to The sTandard cuRve So As tO oBtain THe ligaTe cOncEntratiOn. This mEThoD iS DEScRibed in Japanese PatEnT pUbLication no. 7-6986.
In thE AbOvE-MentioneD MEthOds, iN ORder tO bonD ThE nucleic Acid to THe MaGnetic-rEsPonsibLe ParTicLe, it iS NeceSsary tO form a siLane lAYer to which a bioCOmpatible moleCUlE (FOr EXampLe, a Nucleic acid) Is coVAlenTly bONdEd.
FURtherMore, aN aNAlYZing method and apparaTuS are knOwn utIlizing a sequeNce of a nuclEIC Acid whicH is bONdED to a material seNsitiVe to the magNEtic fielD (WO86/05815). ThE method uTilizes a mAGNetic or mAgnEtiZabLe pARTiCle covered witH A MateRiAl which Is cApable oF beIng BonDinG tO a single sTrand nucLeIc AcId So aS to sePArate and DeTecT tHe sIngle STrand nUcleiC aciD. MOrE SpeCificalLY, tHE SurfAcE oF the MagNeTic paRticLE is Covered With nitroCelLUlosE, wHiCh is a tyPe of cellulose DeRivative, anD nItrOcelluLOSe is specIfically bonded to a single STrand DNa or rNA. ThE sinGle strand dNA Or RNA cOllECted by The metHod is UtIliZED for sEQUeNciNg.
in the methoD, IT is NecesSaRy to SpecifiCally bond the single STraNd DNA or RNA to tHe mAgneTIC cArrIEr.
iT is AlSO knOWN to utilize a poly-Cationic suBsTRaTe for purifYIng, SeParatinG and hybRidizINg a nuclEic acid, esPecially fOr PuriFYIng And separatIng a nuclEic acid coNTainiNG cOntamInaNts (JapAnEse laid-OPen NatIOnaL PublIcatioN NO. 1-502319). In the MethoD, a Sample soLution containing contamiNants iS contActEd with the PolY-CatIonic soliD supPort (magnetIc-rEspOnsibLe pARticlE) so aS To Non-COvaleNtly BOnd the nucLEiC acID To The support wITHOut ExcesSivELy bondIng cOntaminants contaiNEd in the sample sOlutION to The SUppoRt. the support to whIch ThE NUcleic acId Has been bondEd is then SePArateD from tHe soluTion. ExaMPLeS Of the sUppoRt iNclude metAl oXiDE
 ligate concentration incl uding thefollo win g s te ps i s al so known: (1)u sing  a magnetic-responsibl e par ti c le c o nt ainin g super p ar a m agn et ic  ir on ox ide c ove red wit h a polyme ric  s ilane layert owhich a bi oco mpatible mol ecu le isca pab l e ofbei ng bo nded;( 2) rea cting a s am p le sol u tion co n t ai ning  a ligate, a know n  a m ount of a labe led li ga t e, a ndthe  magnetic- re spons i ble par t ic l e tow hich a ligate -specific l i gan d is b on ded ,  so as  to f or m  aligand-liga te c omplex on  the m a gnetic- r esponsi ble pa rti cle ; (3 )  m ag net ic a lly se par a tin g the ma gn et ic-re spon s i b l e pa rti clefromthe reactionsol utio n ; ( 4) me asuri ng t he  labe led li gatewh ich is bonded t o th e magneti c-r es pon si ble p a rticle  or  fr ee labe led lig a tein t h ereaction solution;  a n d  ( 5) apply ing th e  m ea s urementof  th e la b e l lig atet othe stan dard c u rv eso as t oobtain  t helig ate c o ncen tratio n. Thismetho d  is describedi n Japanese Pa t en t Pu b lica tio n No. 7-698 6.
I n  the  abo v e- men t ioned  Meth od s ,i n order to bond the  n ucleic  acid  to the magne tic-respon s i b le parti cle, it is necessary t o for m a silane layer to  whic h a bioc ompatible m olecule(fo r e xam ple , anucleic acid) i s co va lentlybon ded.
Fu rth erm ore , a nanalyzing  methodan dap pa rat us ar e  known u ti liz in g a  sequ e nce of  a nu clei cac i d w hich is bo n d ed t oamate ria lsensi tive tothe mag netic fie ld( WO86 /0 58 15). Th e method util iz es a magne ti c o r magn e t izable p article covered with am aterial  wh ich i s ca pable ofbei ng bon din g  to asingle  stra nd  nu c l eic a c i dsoas  to separa t e  an d det ec t th e singl e strand nucleic a c id.  More specifi cal ly,t h esur f ac e  of  t h e m a g netic particleis covered  w i th  nitrocell u los e,  whichis a ty pe of cellulo se deriva tive, and  n itro c e llu lose is sp ecifical ly bonded to as in gle s tra nd DNA  o r R NA. T he sin g lestran d DNAor  RNA c ollec te d by the  method is utilized for  seque ncing .
I n the met hod ,  it  is neces sary  to specif ica lly  bond  th e  sing le s t ra ndD NA or  RNA to the ma g ne tic c ar rier.
It is a l soknown  to utiliz e apoly-cationic sub s trate for puri fyin g ,  se par a ting  a nd hybridizing  anu c l eic acid ,especiallyfor puri fy i ng an d sepa rating  a nucl e i ca cid co ntai nin g contami nan ts (Japane se  L a id-Ope n Na ti onal P ublica t ionN o . 1-502319). Inthe m e t hod,a  sa mpleso lutionc onta ining cont aminants is  conta cted  with  the po ly -catio nic  s olid suppo r t (magnet ic-re sponsib le  par tic le) so  ast o  non- cova le ntl y bond th e nu c le ic aci d to  thesu ppor t without excessiv ely bonding  c ont a m inants co n t ained in t hesampl e solution t o  the su p port.  The s upport  to whi c h t he  nuclei c a c i d has bee n bondedi s t he n se paratedfr om t he  so lu tion. Examplesof the s upport i n c lude m eta l  oxi d e
 ligate_concentration including_the following steps is_also known:_(1)_using a_magnetic-responsible_particle containing superparamagnetic_iron oxide covered_with a polymeric silane_layer to which_a_biocompatible molecule is capable of being bonded; (2) reacting a sample solution containing a_ligate,_a known_amount_of_a labeled ligate, and the_magnetic-responsible particle to which a_ligate-specific ligand_is bonded, so as to form a ligand-ligate_complex_on the magnetic-responsible_particle; (3) magnetically separating the magnetic-responsible particle from the_reaction solution; (4) measuring the labeled_ligate which is_bonded_to_the magnetic-responsible particle or_free labeled ligate in the reaction_solution; and (5) applying the measurement_of the label ligate to the standard_curve so as to obtain the_ligate concentration. This method is_described in_Japanese Patent Publication No. 7-6986.
In_the above-mentioned Methods,_in order_to bond the_nucleic acid to the magnetic-responsible particle,_it is necessary_to form a silane layer to_which_a biocompatible molecule_(for_example,_a nucleic_acid) is covalently_bonded.
Furthermore,_an analyzing_method_and apparatus are known utilizing a_sequence_of a nucleic acid which is bonded_to a material sensitive_to_the magnetic field (WO86/05815)._The method utilizes a magnetic_or magnetizable particle covered with a_material which_is capable_of being bonding to a single strand nucleic acid so as_to separate and detect the single_strand nucleic acid. More_specifically, the_surface_of the magnetic_particle_is covered_with nitrocellulose, which is a type of_cellulose derivative,_and nitrocellulose is specifically bonded to_a single strand DNA_or_RNA. The single strand DNA or_RNA collected by the method is_utilized for sequencing.
In the method,_it_is_necessary to specifically bond the_single strand DNA or RNA to_the magnetic carrier.
It_is also known to utilize a poly-cationic_substrate_for purifying, separating and hybridizing a_nucleic_acid, especially for purifying and separating_a_nucleic_acid containing contaminants (Japanese Laid-Open_National Publication No. 1-502319). In the_method, a sample solution containing contaminants is contacted with_the poly-cationic solid_support (magnetic-responsible particle) so as_to_non-covalently_bond the nucleic acid to the support without excessively bonding_contaminants contained_in the sample_solution to the support. The support to which the nucleic_acid has been bonded is then separated_from the solution. Examples of the support include metal oxide