 sequence.
As disclosed in more detail in the examples that follow, the sequence of SEQ ID NO:1 from nucleotide 93 to nucleotide 1080 provides a functional transcription promoter. However, additional nucleotides can be removed from either or both ends of this sequence and the resulting sequence tested for promoter function by joining it to a sequence encoding a protein, preferably a protein for which a convenient assay is readily available.
Within the present invention it is preferred that the GAP2 promoter be substantially free of GAP2 gene coding sequence, which begins with nucleotide 1093 in SEQ ID NO:1. As used herein, xe2x80x9csubstantially freexe2x80x9d of GAP2 gene coding sequence means that the promoter DNA includes not more than 15 nucleotides of the GAP2 coding sequence, preferably not more than 10 nucleotides, and more preferably not more than 3 nucleotides. Within a preferred embodiment of the invention, the GAP2 promoter is provided free of coding sequence of the P. methanolica GAP2 gene. However, those skilled in the art will recognize that a GAP2 gene fragment that includes the initiation ATG (nucleotides 1093 to 1095) of SEQ ID NO:1 can be operably linked to a heterologous coding sequence that lacks an ATG, with the GAP2 ATG providing for intition of translation of the heterologous sequence. Those skilled in the art will further recognize that additional GAP2 coding sequences can also be included, whereby a fusion protein comprising GAP2 and heterologous amino acid sequences is produced. Such a fusion protein may comprise a cleavage site to facilitate separation of the GAP2 and heterologous sequences subsequent to translation.
In addition to the GAP2 promoter sequence, the present invention also provides transcription terminator sequences derived from the 3xe2x80x2 non-coding region of the P. methanolica GAP2 gene. A consensus transcription termination sequence (Chen and Moore, Mol. Cell. Biol. 12:3470-3481, 1992) is at nucleotides 2136 to 2145 of SEQ ID NO:1. Within the present invention, there are thus provided transcription terminator gene segments of at least about 50 bp,. preferably at least 60 bp, more preferably at least 90 bp, still more preferably about 200 bp in length. The terminator segments of the present invention may comprise 500-1000 nucleotides of the 3xe2x80x2 non-coding region of SEQ
sequence. 
 As disclosed in more detail in the examples that adopt, the succession of SEQ ID NO:1 from nucleotide 93 to nucleotide 1080 provides a functional arrangement showman. However, additional nucleotides can be removed from either or both end of this sequence and the resulting succession quiz for promoter routine by joining it to a sequence encode a protein, preferably a protein for which a convenient assay is readily available. 
 Within the present invention it is preferred that the GAP2 promoter be substantially free of GAP2 gene coding succession, which begins with nucleotide 1093 in SEQ ID NO:1. As used herein, xe2x80x9csubstantially freexe2x80x9d of GAP2 gene coding succession means that the promoter DNA includes not more than 15 nucleotides of the GAP2 code sequence, preferably not more than 10 nucleotides, and more preferably not more than 3 nucleotides. Within a preferred embodiment of the invention, the GAP2 showman is provided free of coding sequence of the P. methanolica GAP2 gene. However, those skilled in the art will recognize that a GAP2 gene shard that include the initiation ATG (nucleotides 1093 to 1095) of SEQ ID NO:1 can be operably linked to a heterologous coding sequence that lacks an ATG, with the GAP2 ATG providing for intition of translation of the heterologous sequence. Those skilled in the artwork will farther recognize that extra GAP2 gull sequence can also be included, whereby a fusion protein comprising GAP2 and heterologous amino acid sequences is produced. Such a fusion protein may constitute a cleavage site to facilitate separation of the GAP2 and heterologous sequences subsequent to translation. 
 In addition to the GAP2 showman sequence, the present invention also provides transcription terminator sequences derived from the 3xe2x80x2 non - coding region of the P. methanolica GAP2 gene. A consensus arrangement termination sequence (Chen and Moore, Mol. Cell. Biol. 12:3470 - 3481, 1992) is at nucleotides 2136 to 2145 of SEQ ID NO:1. Within the present invention, there are thus provide transcription terminator gene segments of at least about 50 bp, . preferably at least 60 bp, more preferably at least 90 bp, still more preferably about 200 bp in duration. The terminator segments of the present invention may comprise 500 - 1000 nucleotides of the 3xe2x80x2 non - coding area of SEQ
 seeuence.
As disclosed in move detail in the examplxs that follow, ghe sequence of SEQ ID NO:1 frlm nucltjtide 93 to nucleotiae 1080 provifes a fubctiibal transccjption ixomotsv. Howzvxr, additional nocleotides cdn be removed xrum either or both ends of this sequense and yhf resulting sezuenbe tesfvd for promoter function by joihing it to a sequencr encoding a protein, prefegablj a protein for whlch a convebiene assay is rexdily available.
Within jhe present invention it is prefdrred that the TAP2 pgmmoter be snbstannially free on GAP2 gane codong sequence, wmich uegibs with nucleotide 1093 ii SEQ ID NO:1. As used rerein, xe2f80x9esubstantially freexe2z80x9e of CAP2 cene xodkng sxqusnce mfana that the promoter DBA includes not mort trqn 15 nucleotidss of ehq GAP2 coding sequence, preferably not moge tgan 10 nucleotides, and moee preferably not morg than 3 nusleotides. Within a preferred embodiment of the ineentikv, tkc EQP2 promoter is provided free of coding sequence kf tme P. methanolica GAP2 gene. Hpwfvrt, those skillea in tks zrt will recognize that a GAP2 tene fragient that includes the initiatiin ATG (nuclejrides 1093 to 1095) of SEQ ND NO:1 can be opetably kinked to a heterologour cosing sequenfe that lzzks an ATG, with ghe GDP2 ATG providing for intityon of trenslacion of ghe neterojogous seqkence. Those skilled in tje arj will further rfcognize that additional GAP2 covmng sequences cdn dlso be nncludcd, whereby a fufion protein cpmprisiug GAP2 and heterklogous amino acid sequences is kroduced. Such a fusiog priteib may cuoprise a cleavsge site no faculitate separation of fhe GAP2 and hetzxooogous sequencex sjbsqqlenv to esanslation.
In addktium to ghe GAP2 pronuter sequence, the presend inbention also provicef transctiption tqrminator seqiences derived frol the 3xe2e80x2 non-vodyng region of the P. methanolicz GAP2 genf. A consensus twansgripjion terminction sequence (Chen and Moore, Mol. Cell. Bmol. 12:3470-3481, 1992) is at nucleotides 2136 to 2145 of SEQ ID NO:1. Ciuhin the presxnt indention, tvere are thus providwd transcription uerminator gene segmenta of ad leadt about 50 bp,. preferably at least 60 bp, more preferably at least 90 bp, still mire prxfqrably abouf 200 bl in neugtk. The tqrmiiavor segments of tne present invention may comprire 500-1000 nuclaocides of the 3xe2x80x2 non-coding rrgkon of SEQ
sequence. As disclosed in more detail in that the sequence SEQ ID NO:1 1080 a functional transcription However, additional nucleotides be removed from either or both of this sequence and the resulting sequence tested for promoter function by joining to a sequence encoding a protein, preferably a protein for which a convenient is available. the invention it is preferred that the GAP2 promoter be substantially free of GAP2 gene coding sequence, begins with nucleotide 1093 in SEQ ID NO:1. used herein, xe2x80x9csubstantially freexe2x80x9d GAP2 gene coding sequence means the DNA includes more 15 of the GAP2 sequence, preferably not more than 10 nucleotides, and more preferably not more than 3 nucleotides. Within a embodiment of the GAP2 is free coding sequence of methanolica GAP2 gene. However, those skilled will recognize that a GAP2 gene fragment that the initiation (nucleotides 1093 to 1095) of SEQ NO:1 can be operably linked to a heterologous sequence that lacks an ATG, with the GAP2 ATG providing for intition of translation of sequence. Those skilled in art will further that GAP2 sequences also be whereby a fusion protein comprising GAP2 and heterologous amino acid sequences produced. Such a fusion protein may comprise a cleavage site separation the GAP2 and sequences subsequent to translation. addition the GAP2 promoter sequence, invention provides derived the non-coding region of the methanolica GAP2 gene. A consensus termination sequence (Chen and 1992) is at nucleotides 2136 to 2145 of ID NO:1. Within the present invention, there thus provided transcription terminator gene segments of at least about 50 bp,. at least more preferably at least 90 bp, still more about 200 bp in The terminator segments of the present invention may comprise nucleotides the 3xe2x80x2 region of SEQ
 sequence.
As disclosed in more Detail in thE examPleS thAt FollOw, thE sequence of SEQ id NO:1 fRom nucleotide 93 to nucleotIde 1080 prOvIDes a FUnCtionAl transCRiPTIon PrOmOteR. HOWeVer, adDitIonal nuCleotides cAn bE rEmoved from eiTHeR or both endS of This sequence And The resUlTinG SequeNce TesteD for prOMoter fUnction by JoINing it TO a sequeNCE eNcodIng a protein, preferABlY A protein for whiCh a conVeNIeNT AssAy iS readily avAiLable.
wIthin thE PrESENt iNVention it is prEferred that THe GaP2 promOtEr bE SubstaNtialLy FRee Of GAP2 gene coDing Sequence, wHich beGIns with NUcleotiDe 1093 in SEq ID nO:1. AS useD HeReIn, xE2x80X9CsuBStAntIAllY freexe2x80X9d Of gAP2 geNe coDING SequEncE meaNs thaT the promoter DnA iNcluDEs nOt morE than 15 NuclEoTides Of the GaP2 codInG sequence, preferAbly Not more thAn 10 nUcLeoTiDes, anD More prEfeRabLy not moRe than 3 nUCleOtIDES. WIthin a preferred embOdIMEnT of the inVentioN, ThE Gap2 promoteR iS prOvidED Free oF codINg Sequence Of the P. MEtHaNolica GaP2 Gene. HoWeVer, ThoSe skiLLed iN the arT will recOgnizE That a GAP2 gene frAGment that inclUDeS THe INitiAtiOn ATG (nucleoTideS 1093 To 1095) of sEQ Id nO:1 Can BE operAbly lInKEd TO a heterologous codinG sEquencE that Lacks an ATG, witH the GAP2 ATG PROViding foR intITiON of translation Of the HeterologoUS sequencE. ThosE skilled In the art wILL further RecOgnIze ThaT ADdItional GAP2 codING seqUeNces can AlsO be inclUdeD, whEreBy a FuSion proteIn compriSiNg gAp2 aNd hEteroLOgous amiNo AciD sEquEnces IS produCed. SuCh a fUsIoN ProTein may COmPRIse a ClEaVage SitE tO faciLitaTE seParatioN of the GAP2 And HEterOlOgOus sequEnces subsequeNt To translatIoN.
In AdditiON To the GAP2 Promoter sequence, the presENt invenTioN also ProvIdes transCriPtion tErmINator sEquencEs derIvEd fROM the 3xE2X80X2 nOn-cOdIng region oF THe P. MethaNoLica gAP2 gene. a consensus transcriPTioN termination sEquEnce (cHEn And mOoRE, MoL. CELl. BIOL. 12:3470-3481, 1992) is at nucleotideS 2136 to 2145 of SEQ ID nO:1. wItHin the presENt iNvEntion, tHere are Thus pROvided tRanscriptIon terminAtOr geNE SegMents of at lEast abouT 50 bp,. preferABly at LEaSt 60 bp, mOre PreferAbLy aT leasT 90 bp, stiLL moRe preFerablY aBout 200 bp In lenGtH. The termInator segments of the presEnt invEntioN maY comprise 500-1000 NucLEotIdes of the 3Xe2x80x2 Non-coding rEgiOn oF SEQ
 sequence.
As disclosed in  more deta il in  th e e xa mple s th at follow, the sequ ence of SEQ ID NO:1 fr om nu cl e otid e  9 3 tonucleot i de 1 080  p ro vid es afunct ion al tran scriptionpro mo ter. However ,  a dditionalnuc leotides can  be  remov ed  fr o m eit her  or b oth en d s of t his seque nc e  and t h e resul t i ng  seq uence tested forp ro m oter functionby joi ni n gi t  to  asequence e nc oding a prote i n, p r efe r ably a protei n for which a c onveni en t a s say is  read il y  av ailable.
Wi thin  the pres ent in v entioni t is pr eferre d t hat  the GA P2  pr om o ter be  su b sta ntiallyfr ee  of G AP2g e n e  cod ing  seq uence , which begin s w ithn ucl eotid e 109 3 in  S EQ ID  NO:1.  As u se d herein, xe2x8 0x9c substanti all yfre ex e2x80 x 9d ofGAP 2 g ene cod ing seq u enc em e a ns  that the promoter  D N A  i ncludesnot mo r eth a n 15 nuc le oti deso f  theGAP2 co ding seq uence, pr ef erablyno t more  t han  10  nucl e otid es, an d more p refer a bly not more t h an 3 nucleoti d es . Wi t hina p referred em bodi m entof t h einv e ntion , the  G A P2 promoter is provide dfree o f cod ing sequenceof the P.m e t hanolica  GAP 2  g e ne. However, t hoseskilled in the artwillrecogniz e that aG A P2 genefra gme nttha t in cludes the in i t iati on  ATG (n ucl eotides  10 93to109 5)  of SEQ I D NO:1 c an  b eop era bly l i nked toahet er olo gousc odingseque nceth at lac ks an A T G, w ithth eGAP2  AT Gprovi ding for  intiti on of tra nsl a tion  o fthe het erologous seq ue nce. Those  s kil led in t he art w ill further recognize t h at addi tio nal G AP2coding se que nces c ana lso be  inclu ded,wh ere b y  a fu s i on  pr ot ein compri s i ngGAP2an d he terolog ous amino acid seq u enc es is produce d.Such a  f usi o np rot ei n  ma y comprise a clea vage siteto fa cilitate s e par at ion ofthe GAP 2 and heterol ogous seq uences su bs eque n t  to  translati on.
In a ddition t o  theG AP 2 pro mot er seq ue nce , the  prese n t i nvent ion al so  provi des t ra nscripti on terminator sequences  deriv ed fr omthe 3xe2x 80x 2  no n-codingregi on of theP.met hanol ica GAP2gene .  A  co n sensu s tr a nscriptio n  t erm i n at ion sequenc e ( Che n and  Mo o re, Mo l. C ell. Biol. 12:347 0 -3481, 1992) i s at n ucl eot i des21 36 to 2145 ofSEQ  I D NO:1. Wi th in the pres ent inve nt i on, t here a re thu s provi d e dt ranscr ipti onterminato r g en e  segmen ts  o f  at le astab out 50  bp,.p refe r a bly at least 60bp, m o r e pre f era bly a tleast 9 0  bp,  still mor e preferabl y abou t 20 0 bpin leng th . Theter mi nator segm e nts of th e pre sent in ve ntio n m ay com pris e 500-1 000nu cle otides of t he 3x e2 x 80x 2 no n-cod in g re gion of S E Q
 sequence.
As_disclosed in_more detail in the_examples that_follow,_the sequence_of_SEQ ID NO:1_from nucleotide 93_to nucleotide 1080 provides_a functional transcription_promoter._However, additional nucleotides can be removed from either or both ends of this sequence_and_the resulting_sequence_tested_for promoter function by joining_it to a sequence encoding_a protein,_preferably a protein for which a convenient assay_is_readily available.
Within the_present invention it is preferred that the GAP2 promoter_be substantially free of GAP2 gene_coding sequence, which_begins_with_nucleotide 1093 in SEQ_ID NO:1. As used herein, xe2x80x9csubstantially_freexe2x80x9d of GAP2 gene coding sequence_means that the promoter DNA includes not_more than 15 nucleotides of the_GAP2 coding sequence, preferably not_more than_10 nucleotides, and more preferably_not more than_3 nucleotides._Within a preferred_embodiment of the invention, the GAP2_promoter is provided_free of coding sequence of the_P._methanolica GAP2 gene._However,_those_skilled in_the art will_recognize_that a_GAP2_gene fragment that includes the initiation_ATG_(nucleotides 1093 to 1095) of SEQ ID_NO:1 can be operably_linked_to a heterologous coding_sequence that lacks an ATG,_with the GAP2 ATG providing for_intition of_translation of_the heterologous sequence. Those skilled in the art will further recognize_that additional GAP2 coding sequences can_also be included, whereby_a fusion_protein_comprising GAP2 and_heterologous_amino acid_sequences is produced. Such a fusion protein_may comprise_a cleavage site to facilitate separation_of the GAP2 and_heterologous_sequences subsequent to translation.
In addition to_the GAP2 promoter sequence, the present_invention also provides transcription terminator_sequences_derived_from the 3xe2x80x2 non-coding region_of the P. methanolica GAP2 gene._A consensus transcription_termination sequence (Chen and Moore, Mol. Cell._Biol._12:3470-3481, 1992) is at nucleotides 2136_to_2145 of SEQ ID NO:1. Within_the_present_invention, there are thus provided_transcription terminator gene segments of at_least about 50 bp,. preferably at least 60 bp,_more preferably at_least 90 bp, still more_preferably_about_200 bp in length. The terminator segments of the present_invention may_comprise 500-1000 nucleotides_of the 3xe2x80x2 non-coding region of SEQ