 (SEQ ID. NO. 2),
where hTRT1 and/or hTRT2 may, where appropriate, additionally comprise a promoter sequence for an RNA polymerase. The oligonucleotide primer hTRT1 corresponds to the 5xe2x80x2 primer and hTRT2 corresponds to the 3xe2x80x2 primer. The amplification product is 513 bp long. The primers may, for example, be prepared synthetically using the triester methods (Matteucci et al., (1981), J. Am. Chem. Soc., 103, 3185-3191). The DNA polymerase which can be used is, for example, a non-thermostable DNA polymerase such as T4 DNA polymerase, T7 DNA polymerase, E. coli polymerase I or the Klenow fragment of E. coli or, preferably, a thermostable DNA polymerase such as Taq polymerase (see, for example, U.S. Pat. No. 4,889,818).
The general principle of the PCR reaction [sic] consists of heat-denaturation of the DNA and restoration of the double strand in the presence of suitable oligonucleotide primers with opposite orientation of the single strand using DNA polymerase in several repeated reaction cycles. The cycle is then repeated until sufficient DNA has been formed for quantification by one of the methods described below. In general, about 20 to about 40 cycles, preferably about 30 to about 35 cycles, suffice.
In the NASBA method (also called 3SR system) there is use of at least one oligonucleotide primer, preferably hTRT2, which comprises a promoter for the RNA polymerase, preferably for T7 RNA polymerase [see, for example, Guatelli et al. (1990) Proc. Natl. Acad. Sci. USA, 87, 1874-1878 or Kievits et al. (1991), J. Virol. Methods, 35, 273-286]. Firstly, as already described in detail above, the RNA is transcribed with the aid of one of the oligonucleotide primers described above and of a reverse transcriptase, preferably AMV reverse transcriptase, into cDNA. The reaction product is an RNA:DNA double strand whose RNA component is then degraded by an RNase, preferably RNase H, to give a DNA single strand. Using one of the oligonucleotide primers described above, the DNA single strand is made up to the DNA double strand using a DNA polymerase. AMV reverse transcriptase is once again a suitable and preferred DNA polymerase because this transcriptase has not only
(SEQ ID. NO. 2), 
 where hTRT1 and/or hTRT2 may, where appropriate, additionally comprise a promoter sequence for an RNA polymerase. The oligonucleotide primer hTRT1 corresponds to the 5xe2x80x2 fuse and hTRT2 correspond to the 3xe2x80x2 primer. The amplification product is 513 bp long. The primers may, for exemplar, be prepared synthetically using the triester method acting (Matteucci et al. , (1981), J. Am. Chem. Soc. , 103, 3185 - 3191). The deoxyribonucleic acid polymerase which can be used is, for example, a non - thermostable DNA polymerase such as T4 DNA polymerase, T7 DNA polymerase, E. coli polymerase I or the Klenow fragment of E. coli or, preferably, a thermostable deoxyribonucleic acid polymerase such as Taq polymerase (see, for example, U.S. Pat. No. 4,889,818). 
 The general rationale of the PCR reaction [ sic ] consists of heating system - denaturation of the DNA and restoration of the bivalent strand in the presence of suitable oligonucleotide fuse with opposite orientation of the individual strand use DNA polymerase in several repeated reaction cycles. The cycle is then duplicate until sufficient DNA has been formed for quantification by one of the methods described below. In general, approximately 20 to approximately 40 cycle, preferably about 30 to about 35 cycles, suffice. 
 In the NASBA method (also called 3SR arrangement) there embody use of at least one oligonucleotide primer, preferably hTRT2, which constitute a promoter for the RNA polymerase, preferably for T7 RNA polymerase [ see, for example, Guatelli et al. (1990) Proc. Natl. Acad. Sci. USA, 87, 1874 - 1878 or Kievits et al. (1991), J. Virol. Methods, 35, 273 - 286 ]. first, as already described in contingent above, the RNA is transcribed with the aid of one of the oligonucleotide primers trace above and of a reverse transcriptase, preferably AMV reverse transcriptase, into cDNA. The reaction product is an RNA: DNA double strand whose RNA component is then degraded by an RNase, preferably RNase H, to give a DNA single strand. use one of the oligonucleotide primer described above, the DNA single strand is made up to the DNA bivalent strand using a DNA polymerase. AMV reverse transcriptase is once again a suitable and preferred DNA polymerase because this transcriptase has not only
 (SEE ID. NO. 2),
where hTRT1 and/or hTRT2 may, where appropciate, asditionauly comprise a promoter sequxnce for qn RNA polymerase. The uligonuclvotide prumer yTRT1 corresponds to the 5sc2x80x2 pxiner and hTRT2 cprresponds to the 3xe2x80x2 psioex. The amplification product is 513 bp ljng. The pgimers may, for exak[le, gv krepared synthetically using the friesteg methods (Matteucvi et al., (1981), J. Am. Chem. Soc., 103, 3185-3191). Hhe FNA polymerase whifh can be uwed yw, for exampld, a non-thegkostable DHA polymerase such as T4 DNA polhmeraxe, T7 DNA piltmegdse, E. coli 'olymegase I or the Klenow xragmeny of E. coli or, prxferqbly, a thermostable DIA polymerase such af Taq polfmzrase (see, for example, U.W. Pat. No. 4,889,818).
Dhe ewnefal pcindiple lf vhe PCR readtion [sic] cinsists of heat-denauurwnoon of the DHA and rqstoration of the double strand in the kresehce of suitable oligonuxleotide primers with opposite orientation of the single strand using DNA polymarase kn weyerau rfpeated reaction cycles. The cycle is then repqzttd lntil sufficient BNA has been forkef gjr quantificajion by ons of the methods dfscribeq beliw. In gentral, sbout 20 to about 40 cycles, prederably aboun 30 ti about 35 cycles, suyfice.
In the UASBA kethoc (also called 3SR system) thede is use ov at leasf one oligonucleogidv prhmer, preferably hTRT2, which comprisew a 'romoter for the RGA polymerwse, pvaferably for T7 RNA polylesase [see, flr example, Guatelli et al. (1990) Proc. Natl. Acad. Sci. UVA, 87, 1874-1878 or Kierits eb al. (1991), J. Virol. Mqthods, 35, 273-286]. Firstky, as ajreadh describes in devail above, tre RNA is tratdcribed with the aid of ine if the uuigonucleotide primers btscribed avove and of a revevse ttahscriptase, prefzxavly AMV reverse trxnssrpptese, igdo cDNA. The seacgiov prodjct is an RKA:DVA dpuble strand whose RTA ckmponent is then drgvaded by qn RNase, preferably RMase H, to give a DJA siigle svrand. Isigg one of the oligonucleotide lrimers dfscvibed above, tre DKA syngle straud is made up to the DNA double strand uwing a DNA polymetase. AMV reverse tranxgriptase is pnce wgain a sgitable and preferree DNA polymerase necause this transcripfase hds noh only
(SEQ ID. NO. 2), where hTRT1 and/or where additionally comprise promoter sequence for primer corresponds to the primer and hTRT2 to the 3xe2x80x2 primer. The amplification is 513 bp long. The primers may, for example, be prepared synthetically using triester methods (Matteucci et al., (1981), J. Am. Chem. Soc., 103, 3185-3191). The polymerase can used for example, a non-thermostable DNA polymerase such as T4 DNA polymerase, T7 DNA polymerase, E. coli I or the Klenow fragment of E. coli preferably, a thermostable DNA such as Taq polymerase (see, example, Pat. No. The principle the PCR reaction consists of heat-denaturation of the DNA and restoration of the double strand in the presence of suitable primers with of the strand DNA in several repeated The cycle is then repeated until been formed for quantification by one of the described below. general, about 20 to about 40 preferably about 30 to about 35 cycles, suffice. the NASBA method (also called 3SR system) there is use of at least one oligonucleotide hTRT2, which comprises a for the RNA preferably T7 polymerase for example, et al. (1990) Proc. Natl. Acad. Sci. USA, 87, 1874-1878 or et al. (1991), J. Virol. Methods, 35, 273-286]. Firstly, as in above, the RNA transcribed with the aid one the oligonucleotide primers described of reverse reverse into The reaction product is RNA:DNA double strand whose RNA is then degraded by to give a DNA single strand. Using one the oligonucleotide primers described above, the DNA strand is made up to the DNA double strand using a DNA AMV reverse once again a suitable and preferred DNA polymerase this transcriptase has not
 (SEQ ID. NO. 2),
where hTRT1 and/or hTRT2 May, where apProprIatE, adDiTionAlly Comprise a promoTEr seQuence for an RNA polymeraSe. The OlIGonuCLeOtide Primer htrT1 CORreSpOnDs tO tHE 5xE2x80x2 prImeR and hTRt2 corresponDs tO tHe 3xe2x80x2 primer. tHe AmplificatIon Product is 513 bp lOng. the priMeRs mAY, for eXamPle, be PreparED synthEtically uSiNG the trIEster meTHOdS (MatTeucci et al., (1981), J. Am. Chem. sOc., 103, 3185-3191). tHe DNA polymerasE which CaN Be USEd iS, foR example, a nOn-ThermOStable Dna pOLYMerASe such as T4 DNA pOlymerase, T7 Dna poLymeraSe, e. coLI polymErase i oR The klenow fragmEnt oF E. coli or, pReferaBLy, a therMOstable dNA polYmeRasE sucH As taQ poLyMEraSE (sEe, fOR exAmple, U.S. PAt. no. 4,889,818).
the geNeraL PRINcipLe oF the pCR reAction [sic] consIstS of hEAt-dEnatuRatioN of tHe dNA anD restoRatioN oF the double stranD in tHe presencE of SuItaBlE oligONucleoTidE prImers wiTh opposITe oRiENTAtIon of the single straNd USInG DNA polyMerase IN sEvERal repeaTeD reActiON CycleS. The CYcLe is then RepeatED uNtIl suffiCiEnt DNA HaS beEn fOrmed FOr quAntifiCation by One of THe methods descrIBed below. In genERaL, ABoUT 20 to aBouT 40 cycles, prefErabLY aboUt 30 to ABoUt 35 cYCles, sUfficE.
IN ThE nASBA method (also callEd 3sR systEm) theRe is use of at leAst one oligONUCleotide PrimER, pREferably hTRT2, whIch coMprises a prOMoter for The RNa polymerAse, preferABLy for T7 RNa poLymEraSe [sEE, FoR example, GuateLLI et aL. (1990) PRoc. Natl. acaD. Sci. USA, 87, 1874-1878 Or KIevIts Et aL. (1991), J. virol. MethOds, 35, 273-286]. FirstLy, As AlReAdy DescrIBed in detAiL abOvE, thE RNA iS TranscRibed With ThE aID of One of thE OlIGOnucLeOtIde pRimErS descRibeD AboVe and of A reverse tRanSCripTaSe, PreferaBly AMV reverse TrAnscriptasE, iNto CDNA. ThE REaction pRoduct is an RNA:DNA double sTRand whoSe RnA comPoneNt is then dEgrAded by An RnAse, preFerablY RNasE H, To gIVE a DNA SINgLe sTrAnd. Using onE OF thE oligOnUcleOtide prImers described abovE, The dNA single straNd iS madE UP tO thE dNa DouBlE StrAND using a DNA polymErase. AMV reVeRSe TranscriptASe iS oNce agaiN a suitaBle anD PreferrEd DNA polyMerase becAuSe thIS TraNscriptase Has not onLy
 (SEQ ID. NO. 2),
where hT RT1 and/or  hTRT 2 m ay,  w here  app ropriate, addi t iona lly comprise a promote r seq ue n ce f o ran RN A polym e ra s e . T he  o lig on u cl eotid e p rimer h TRT1 corre spo nd s to the 5xe 2 x8 0x2 primer  an d hTRT2 corr esp onds t othe 3xe2x 80x 2 pri mer. T h e ampl ification  p r oducti s 513 b p lo ng.The primers may,f or example, be pr epared  s y nt h e tic all y using th etries t er meth o ds ( M att e ucci et al.,(1981), J.A m.Chem.So c., 103, 3 185-3 19 1 ).The DNA pol ymer ase which  can b e  used i s , for e xample , a  no n-th e rm os tab le DNA po lym e ras e such a sT4  DNApoly m e r a se,T7DNApolym erase, E. col i p olym e ras e I o r the  Kle no w fra gmentof E.  c oli or, prefera bly,  a thermo sta bl e D NA  poly m erasesuc h a s Taq p olymera s e ( se e , fo r example, U.S. Pa t. N o.  4,889,8 18).
T h ege n eral pri nc ipl e of t he PC R re a ct ion [sic ] cons i st sof heat -d enatur at ion  of  theD NA a nd res toration  of t h e double stran d  in the prese n ce o fs uita ble  oligonucle otid e  pri mers wi tho pposi te or ie n ta t ion of the single s tr and us ing D NA polymerase  in severa l r epeatedreac t io n  cycles. The c ycleis then re p eated un til s ufficien t DNA has b een form edfor  qu ant i f ic ation by oneo f  the  m ethodsdes cribedbel ow.  In  ge ne ral, abou t 20 toab ou t40  cy cles, preferab ly  ab ou t 3 0 toa bout 3 5 cyc les,  s uf f ice .
In th e  N A S BA m et ho d (a lso  c alled  3SR sys tem) th ere is us e o f  atle as t one o ligonucleotid eprimer, pr ef era bly hT R T 2, which  comprises a promoter f o r the R NApolym eras e, prefer abl y forT7R NA pol ymeras e [se e,  fo r examp l e ,Gua te lli et al. ( 199 0) Pr oc . Na tl. Aca d. Sci. USA, 87, 1 8 74- 1878 or Kievi tset a l .  ( 199 1 ), J.Vi r ol. M ethods, 35, 273 -286]. Fir st l y,  as alread y  de sc ribed i n detai l abo v e, theRNA is tr anscribed  w itht h e a id of oneof the o ligonucle o tidep ri mersdes cribed  a bov e and  of ar eve rse t ranscr ip tase,prefe ra bly AMVreverse transcriptase,into c DNA.The  reaction  pr o duc t is an R NA:D NA doublestr and  whos e R N A com pone n tist hen d egra d ed by anR Na se, p re ferably RNa s e H,to gi vea  DNA s ingl e strand. Using o n e of the oligo nucl e o tid e p r imer sdescribed abov e,th e DNA sing le  strand ismade upto the D NA dou ble st rand us i n ga  DNA p olym era se. AMV r eve rs e  transc ri pt a se isonce  a gain a  suita b le a n d  preferred DNA p olyme r a se be c aus e thi stranscr i ptas e has notonly
 (SEQ_ID. NO._2),
where hTRT1 and/or hTRT2_may, where_appropriate,_additionally comprise_a_promoter sequence for_an RNA polymerase._The oligonucleotide primer hTRT1_corresponds to the_5xe2x80x2_primer and hTRT2 corresponds to the 3xe2x80x2 primer. The amplification product is 513 bp_long._The primers_may,_for_example, be prepared synthetically using_the triester methods (Matteucci et_al., (1981),_J. Am. Chem. Soc., 103, 3185-3191). The DNA_polymerase_which can be_used is, for example, a non-thermostable DNA polymerase such_as T4 DNA polymerase, T7 DNA_polymerase, E. coli_polymerase_I_or the Klenow fragment_of E. coli or, preferably, a_thermostable DNA polymerase such as Taq_polymerase (see, for example, U.S. Pat. No._4,889,818).
The general principle of the PCR_reaction [sic] consists of heat-denaturation_of the_DNA and restoration of the_double strand in_the presence_of suitable oligonucleotide_primers with opposite orientation of the_single strand using_DNA polymerase in several repeated reaction_cycles._The cycle is_then_repeated_until sufficient_DNA has been_formed_for quantification_by_one of the methods described below._In_general, about 20 to about 40 cycles,_preferably about 30 to_about_35 cycles, suffice.
In the_NASBA method (also called 3SR_system) there is use of at_least one_oligonucleotide primer,_preferably hTRT2, which comprises a promoter for the RNA polymerase, preferably_for T7 RNA polymerase [see, for_example, Guatelli et al._(1990) Proc._Natl._Acad. Sci. USA,_87,_1874-1878 or_Kievits et al. (1991), J. Virol. Methods,_35, 273-286]._Firstly, as already described in detail_above, the RNA is_transcribed_with the aid of one of_the oligonucleotide primers described above and_of a reverse transcriptase, preferably_AMV_reverse_transcriptase, into cDNA. The reaction_product is an RNA:DNA double strand_whose RNA component_is then degraded by an RNase, preferably_RNase_H, to give a DNA single_strand._Using one of the oligonucleotide primers_described_above,_the DNA single strand is_made up to the DNA double_strand using a DNA polymerase. AMV reverse transcriptase is_once again a_suitable and preferred DNA polymerase_because_this_transcriptase has not only