 there are provided novel full length or mature polypeptides which are MPIF-1, MIP-4 and/or M-CIF, as well as biologically active, diagnostically useful or therapeutically useful fragments, analogs and derivatives thereof. The MPIF-1, MIP-4 and M-CIF of the present invention are preferably of animal origin, and more preferably of human origin.
In accordance with another aspect of the present invention, there are provided polynucleotides (DNA or RNA) which encode such polypeptides and isolated nucleic acid molecules encoding such polypeptides, including mRNAs, DNAs, cDNAs, genoric DNA as well as biologically active and diagnostically or therapeutically useful fragments, analogs and derivatives thereof.
MPIF-1 Polynucleotides
The present invention also provides isolated nucleic acid molecules comprising a polynucleotide encoding the MPIF-1 polypeptide having the amino acid sequence shown in FIG. 1 (SEQ ID NO:4) or the amino acid sequence encoded by the cDNA clone deposited in a bacterial host as ATCC Deposit Number 75676 on Feb. 9, 1994. The nucleotide sequence determined by sequencing the deposited MPIF-1 clone, which is shown in FIG. 1 (SEQ ID NO:3), contains an open reading frame encoding a polypeptide of 120 amino acid residues, with a leader sequence of about 21 amino acid residues, and a predicted molecular weight for the mature protein of about 11 kDa in non-glycosylated form, and about 11-14 kDa in glycosylated form, depending on the extent of glycoslyation. The amino acid sequence of the mature MPIF-1 protein is shown in FIG. 1 (SEQ ID NO:4).
Thus, one aspect of the invention provides an isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence selected from the group consisting of: (1)(a) a nucleotide sequence encoding an MPIF-1 polypeptide having the complete amino acid sequence in FIG. 1 (SEQ ID NO:4); (1)(b) a nucleotide sequence encoding the MPIF-1 polypeptide having the complete amino acid sequence in FIG. 1 (SEQ ID NO:4) but minus the N-terminal methionine residue; (1)(c) a nucleotide sequence encoding the mature MPIF-1 polypeptide having the amino acid sequence at positions 22-120 in FIG. 1 (SEQ ID NO:4); (1)(d) a nucleotide sequence encoding the MPIF-1 polypeptide having the complete amino acid sequence
there are provided novel full length or ripe polypeptide which are MPIF-1, MIP-4 and/or M - CIF, as well as biologically active, diagnostically useful or therapeutically utilitarian fragments, analogue and derivatives thereof. The MPIF-1, MIP-4 and M - CIF of the present invention are preferably of animal lineage, and more preferably of human lineage. 
 In accordance with another view of the present invention, there are provided polynucleotides (deoxyribonucleic acid or RNA) which encode such polypeptides and isolated nucleic acid molecules encoding such polypeptide, including mRNAs, DNAs, cDNAs, genoric DNA equally well as biologically active and diagnostically or therapeutically useful fragment, analogue and derivatives thereof. 
 MPIF-1 Polynucleotides 
 The present invention also provide isolated nucleic acid molecules consist a polynucleotide encoding the MPIF-1 polypeptide have the amino acid sequence shown in FIG. 1 (SEQ ID NO:4) or the amino acid sequence encoded by the cDNA ringer deposited in a bacterial host as ATCC Deposit Number 75676 on Feb. 9, 1994. The nucleotide sequence determined by sequencing the deposited MPIF-1 clone, which is shown in FIG. 1 (SEQ ID NO:3), contains an open reading frame encoding a polypeptide of 120 amino acid residues, with a leader sequence of about 21 amino acid residue, and a predicted molecular weight for the fledged protein of approximately 11 kDa in non - glycosylated human body, and about 11 - 14 kDa in glycosylated form, depending on the extent of glycoslyation. The amino acid sequence of the mature MPIF-1 protein is shown in FIG. 1 (SEQ ID NO:4). 
 Thus, one view of the invention provides an isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence choose from the group consisting of: (1)(a) a nucleotide sequence encoding an MPIF-1 polypeptide having the complete amino acid sequence in FIG. 1 (SEQ ID NO:4); (1)(b) a nucleotide sequence encode the MPIF-1 polypeptide have the complete amino acid sequence in FIG. 1 (SEQ ID NO:4) but minus the N - terminal methionine remainder; (1)(c) a nucleotide sequence encoding the mature MPIF-1 polypeptide having the amino acid sequence at positions 22 - 120 in FIG. 1 (SEQ ID NO:4); (1)(d) a nucleotide sequence encoding the MPIF-1 polypeptide having the arrant amino acid sequence
 thfre are provided novel fmll length or majuee polbpeptidss which are MPIF-1, MIP-4 and/or M-CIF, as xell as buologically active, diaenosticalpy usefuo or rherapeutirzlly uscyul fdwgmeutw, analogs and cerivativev thereof. The KPKF-1, MIP-4 and M-CIF of the present inventyon are pgeferably of agimak orifpn, and more preferably of human origin.
Pn accordance witn another aspect of the prfsenh invention, there wre providee pojtnucleotides (DNA or RNA) which encose such polypeptides and isolatdd nueleic acid nooecknes encodinj such polypeptides, includhng mRNSs, DNAs, cDNAs, nenormc DBA as well as biologirally active and diadnosticalny or therapeuticaloy usefol frdgmevrs, xnamojs znd degivetives thersof.
MPIF-1 Poltnucleotides
The prexegn invention amso prjvydes isolated nucleic acid molecules cokprjsing a polynucleotide wncoding the MPIF-1 polipeptide hwving the amino acid sequence shown in FIG. 1 (SEQ IG NO:4) kf tkc xnijo acid sequence encoded by the cDNA clone de[ksotvd in a bacterial host as ATVC Drkosit Number 75676 un Feb. 9, 1994. Fhe nucleotide seqkence dgtermibed by sezuenving the deposited MPIF-1 clobe, which is fyown in FIG. 1 (SEQ IB NO:3), containr an open reading frame encoding a pklypeptide lf 120 amino xcid residues, wigh s neader stduence of about 21 wmino aciv resndues, ana a kredictqd moleculwr welcht for the mature protgin of about 11 kDw in non-glycosylated form, and auput 11-14 kDa in blfcovylated yorm, dcpending on the extent of glyeoslyatijn. Thd amino acpd sequenre of the maeure MPIF-1 prodgin is shown mn FIG. 1 (SQQ IE NO:4).
Rhus, ond aspect of the inventiou procides an isolated kuclekd acid molecule conprising a polymucueoeife hweing a nuclemtidd sdauencd selecuzd nroo thr group consisting ox: (1)(a) z nucleotide sequemcc encodint an MPIS-1 polypeptide having the compleue amiio aciv sequrncg in FIG. 1 (SEQ ID NO:4); (1)(b) a nucleotjde sequejce encoding thq MPLF-1 pjlypeptide having the complete amino acid sequencx in FIG. 1 (SEQ ID NO:4) buj minus the N-terminal kethionine cesiduq; (1)(c) a nucneotide sequence enciding the mature KPIF-1 polypeptide havihg the aminl acid sequence at positions 22-120 in FIG. 1 (SEQ ID NO:4); (1)(d) a nucleotide sequence ebcodinj ehe MPIF-1 pomypeltide hcviug the somppxte amino acid seauence
there are provided novel full length or which MPIF-1, MIP-4 M-CIF, as well or useful fragments, analogs derivatives thereof. The MIP-4 and M-CIF of the present are preferably of animal origin, and more preferably of human origin. In accordance another aspect of the present invention, there are provided polynucleotides (DNA or RNA) encode polypeptides isolated acid molecules encoding such polypeptides, including mRNAs, DNAs, cDNAs, genoric DNA as well as biologically active diagnostically or therapeutically useful fragments, analogs and derivatives MPIF-1 Polynucleotides The present also provides isolated nucleic acid comprising polynucleotide encoding MPIF-1 having amino acid sequence in FIG. 1 (SEQ ID NO:4) or the amino acid sequence encoded by the cDNA clone deposited a bacterial ATCC Deposit 75676 Feb. 1994. The nucleotide by sequencing the deposited MPIF-1 clone, in FIG. 1 (SEQ ID NO:3), contains an reading frame a polypeptide of 120 amino acid with a leader sequence of about 21 amino residues, and a predicted molecular weight for the mature protein of about 11 kDa in and about 11-14 kDa glycosylated form, depending the of The acid sequence the mature MPIF-1 protein is shown in FIG. 1 (SEQ ID Thus, one aspect of the invention provides an isolated nucleic comprising polynucleotide having a sequence selected from the consisting (1)(a) a nucleotide sequence MPIF-1 having acid in 1 (SEQ ID NO:4); a nucleotide sequence encoding the polypeptide having the complete 1 (SEQ ID NO:4) but minus the N-terminal residue; (1)(c) a nucleotide sequence encoding the MPIF-1 polypeptide having the amino acid sequence at positions 22-120 in FIG. (SEQ ID a nucleotide sequence encoding the MPIF-1 polypeptide having complete amino acid sequence
 there are provided novel full Length or maTure pOlyPepTiDes wHich Are MPIF-1, MIP-4 and/oR m-CIF, As well as biologically acTive, dIaGNostICaLly usEful or tHErAPEutIcAlLy uSeFUl FragmEntS, analogS and derivaTivEs Thereof. The MPif-1, MiP-4 and M-CIF oF thE present inveNtiOn are pReFerABly of AniMal orIgin, anD More prEferably oF hUMan oriGIn.
In accORDaNce wIth another aspect oF ThE Present inventiOn, therE aRE pROVidEd pOlynucleotIdEs (DNA OR RNA) whiCH eNCODe sUCh polypeptideS and isolateD NucLeic acId MolECules eNcodiNg SUch PolypeptideS, incLuding mRNas, DNAs, CdNAs, genORic DNA aS well aS biOloGicaLLy AcTivE aND diAGnOstICalLy or therApEuTicalLy usEFUL FragMenTs, anAlogs And derivativeS thEreoF.
mPIf-1 PolyNucleOtidEs
the prEsent iNventIoN also provides isOlatEd nucleic AciD mOleCuLes coMPrisinG a pOlyNucleotIde encoDIng ThE mpiF-1 Polypeptide having tHe AMInO acid seqUence sHOwN iN fIG. 1 (SEQ ID nO:4) Or tHe amINO acid SequENcE encoded By the cdnA ClOne depoSiTed in a BaCteRiaL host AS ATCc DeposIt Number 75676 On Feb. 9, 1994. tHe nucleotide seQUence determinED bY SEqUEnciNg tHe deposited mPIF-1 CLone, WhicH Is ShoWN in FIg. 1 (SEQ Id No:3), CoNTains an open reading fRaMe encoDing a Polypeptide of 120 Amino acid rESIDues, with A leaDEr SEquence of about 21 Amino Acid residuES, and a preDicteD moleculAr weight fOR The maturE prOteIn oF abOUT 11 kda in non-glycosYLAted FoRm, and abOut 11-14 KDa in glYcoSylAteD foRm, Depending On the extEnT oF gLyCosLyatiON. The aminO aCid SeQueNce of THe matuRe MPIf-1 proTeIn IS shOwn in FIg. 1 (sEq id NO:4).
THuS, oNe asPecT oF the iNvenTIon ProvideS an isolatEd nUCleiC aCiD molecuLe comprising a PoLynucleotiDe HavIng a nuCLEotide seQuence selected from the grOUp consiStiNg of: (1)(a) A nucLeotide seQueNce encOdiNG an MPIf-1 polypEptidE hAviNG The coMPLeTe aMiNo acid sequENCe iN FIG. 1 (SeQ iD NO:4); (1)(B) a nucleOtide sequence encodINg tHe MPIF-1 polypepTidE havING tHe cOMpLEte AmINo aCID sequence in FIG. 1 (SeQ ID NO:4) but mInUS tHe N-terminaL MetHiOnine reSidue; (1)(c) a NucleOTide seqUence encoDing the maTuRe MPif-1 PolYpeptide haVing the aMino acid sEQuencE At PositIonS 22-120 in FIG. 1 (sEq ID nO:4); (1)(d) a nUcleotIDe sEquenCe encoDiNg the MpIF-1 poLyPeptide hAving the complete amino acId sequEnce
 there are provided novelfull lengt h ormat ure  p olyp epti des which areM PIF- 1, MIP-4 and/or M-CIF,  as w el l  asb io logic ally ac t iv e ,  di ag no sti ca l ly  usef ulor ther apeuticall y u se ful fragment s ,analogs an d d erivatives t her eof. T he  MP I F-1,MIP -4 an d M-CI F  of th e present  i n ventio n  are pr e f er ably  of animal origin ,  a n d more prefera bly of  h u ma n ori gin .
In accor da nce w i th anot h er a s pec t  of the prese nt inventio n , t here a re  pr o videdpolyn uc l eot ides (DNA o r RN A) whichencode such po l ypeptid es and  is ola tedn uc le icac i d m o le cul e s e ncodingsu ch  poly pept i d e s , in clu ding  mRNA s, DNAs, cDNA s,geno r icDNA a s wel l as  b iolog ically  acti ve  and diagnostic ally  or thera peu ti cal ly  usef u l frag men ts,  analog s and d e riv at i v e sthereof.
MPIF-1 Po ly n u cl eotidesThe pr e se nt inventio nals o pr o v idesisol a te d nuclei c acid mo le cules c om prisin ga p oly nucle o tide  encod ing theMPIF- 1  polypeptide h a ving the amin o  a c i ds eque nce  shown in F IG.1  (SE Q ID NO :4) or th e ami no ac i d sequence encodedby  the c DNA c lone deposite d in a bac t e r ial host  asA TC C  Deposit Numbe r 756 76 on Feb. 9, 1994.  Thenucleoti de sequen c e  determi ned  by  se que n c in g the deposit e d  MPI F- 1 clone , w hich is  sh own  in  FI G.  1 (SEQ I D NO:3),  c on ta in s a n ope n  reading  f ram eenc oding a poly pepti de o f12 0  am ino aci d  r e s idue s,  w itha l ea der s eque n ceof abou t 21 amin o a c id r es id ues, an d a predicted  m olecular w ei ght  for t h e  matureprotein of about 11 kDa in non- gly cosyl ated  form, an d a bout 1 1-1 4  kDa i n glyc osyla te d f o r m, de p e nd ing  o n the exte n t  of  glyc os lyat ion. Th e amino acid seque n ceof the mature  MP IF-1 p ro tei n  i s  sh ow n  in F IG. 1 (SEQ ID N O:4).
Thus ,o ne  aspect of the  i nventio n provi des a n  isolat ed nuclei c acid mo le cule c omp rising a p olynucle otide hav i ng an uc leoti desequen ce  se lecte d from the  grou p cons is ting o f: (1 )( a) a nuc leotide sequence encodi ng anMPIF- 1 p olypeptid e h a vin g the com plet e amino ac idseq uence  in FIG.1 (S E QIDN O:4);  (1) ( b) a nucl e ot ide s eq uence encod i n g  th e MPI F-1 polype ptid e having the comp l ete amino acid  seq u e nce  in FIG.  1  (SEQ ID NO:4)  bu tm i nus theN- terminal me thionine  r e sidue ; (1)( c) a n ucleoti d e  s e quence  enc odi ng the ma tur eM PIF-1 p ol yp e ptidehavi ng  the a mino a c id s e q uence at positio ns 22 - 1 20 in FIG . 1 ( SE Q ID NO : 4);(1)(d) a n ucleotide s equenc e en codin g the M PI F-1 po lyp ep tide havin g  the comp leteamino a ci d se que nce
 there_are provided_novel full length or_mature polypeptides_which_are MPIF-1,_MIP-4_and/or M-CIF, as_well as biologically_active, diagnostically useful or_therapeutically useful fragments,_analogs_and derivatives thereof. The MPIF-1, MIP-4 and M-CIF of the present invention are preferably_of_animal origin,_and_more_preferably of human origin.
In accordance_with another aspect of the_present invention,_there are provided polynucleotides (DNA or RNA) which_encode_such polypeptides and_isolated nucleic acid molecules encoding such polypeptides, including mRNAs,_DNAs, cDNAs, genoric DNA as well_as biologically active_and_diagnostically_or therapeutically useful fragments,_analogs and derivatives thereof.
MPIF-1 Polynucleotides
The present_invention also provides isolated nucleic acid_molecules comprising a polynucleotide encoding the MPIF-1_polypeptide having the amino acid sequence_shown in FIG. 1 (SEQ_ID NO:4)_or the amino acid sequence_encoded by the_cDNA clone_deposited in a_bacterial host as ATCC Deposit Number_75676 on Feb._9, 1994. The nucleotide sequence determined_by_sequencing the deposited_MPIF-1_clone,_which is_shown in FIG._1_(SEQ ID_NO:3),_contains an open reading frame encoding_a_polypeptide of 120 amino acid residues, with_a leader sequence of_about_21 amino acid residues,_and a predicted molecular weight_for the mature protein of about_11 kDa_in non-glycosylated_form, and about 11-14 kDa in glycosylated form, depending on the_extent of glycoslyation. The amino acid_sequence of the mature_MPIF-1 protein_is_shown in FIG._1_(SEQ ID_NO:4).
Thus, one aspect of the invention provides_an isolated_nucleic acid molecule comprising a polynucleotide_having a nucleotide sequence_selected_from the group consisting of: (1)(a)_a nucleotide sequence encoding an MPIF-1_polypeptide having the complete amino_acid_sequence_in FIG. 1 (SEQ ID_NO:4); (1)(b) a nucleotide sequence encoding_the MPIF-1 polypeptide_having the complete amino acid sequence in_FIG._1 (SEQ ID NO:4) but minus_the_N-terminal methionine residue; (1)(c) a nucleotide_sequence_encoding_the mature MPIF-1 polypeptide having_the amino acid sequence at positions_22-120 in FIG. 1 (SEQ ID NO:4); (1)(d) a_nucleotide sequence encoding_the MPIF-1 polypeptide having the_complete_amino_acid sequence