 the recruitment or trafficking of leukocyte cell populations. ChemerinR, by its specific expression in macrophages and immature dendritic cells, appears as a particularly attractive candidate receptor involved in the initiation and early regulation of immune responses.
TIG2 (Tazarotene-induced gene 2, thereafter Preprochemerin [Sequence ID Nos: 7 (human Preprochemerin polynucleotide sequence, FIG. 6); 8 (human amino acid sequence, FIG. 6); 9 (mouse polynucleotide sequence, FIG. 7); and 10 (mouse amino acid sequence, FIG. 7)] was identified as a cDNA, the expression of which is up-regulated by the treatment of skin raft cultures by the retinoic acid receptor (RAR) beta/gamma-selective anti-psoriatic synthetic retinoid, tazarotene [AGN 190168/ethyl 6-[2-(4,4-dimethylthiochroman-6-yl)-ethynyl]nicotinate] (Nagpal S, Patel S, Jacobe H, DiSepio D, Ghosn C, Malhotra M, Teng M, Duvic M, Chandraratna R A. (1997) J Invest Dermatol 109: 91-5). The retinoid-mediated up-regulation in the expression of Preprochemerin was confirmed by Northern blot analysis. The Preprochemerin is located at 17p13.3 position, a region associated with pancretic tumorigenesis. The Preprochemerin cDNA is 830 bp long and encodes a putative protein product of 163 amino acids. Preprochemerin is expressed and induced by tazarotene in culture only when keratinocytes and fibroblasts form a tissue-like 3-dimensional structure. RAR-specific retinoids were also shown to increase Preprochemerin mRNA levels. In contrast, neither RXR-specific retinoids nor 1,25-dihydroxyvitamin D3 increased Preprochemerin levels in these cells. Preprochemerin is also expressed at high levels in nonlesional psoriatic skin but at lower levels in the psoriatic lesion and its expression is up-regulated in psoriatic lesions after topical application of tazarotene. In addition, Preprochemerin has been shown to be dramatically upregulated by 1,25 dihydroxyvitamin D3 and dexamethasone in osteoclast-supporting stromal cells (Adams A E, Abu-Amer Y, Chapp
the recruitment or trafficking of leukocyte cell populations. ChemerinR, by its specific expression in macrophages and unfledged dendritic cell, appears as a particularly attractive candidate sense organ involved in the initiation and early regulation of immune response. 
 TIG2 (Tazarotene - induced gene 2, thereafter Preprochemerin [ Sequence ID Nos: 7 (human Preprochemerin polynucleotide sequence, FIG. 6); 8 (human amino acid succession, FIG. 6); 9 (shiner polynucleotide sequence, FIG. 7); and 10 (mouse amino acid sequence, FIG. 7) ] was identify as a cDNA, the expression of which is up - regulated by the discussion of skin raft acculturation by the retinoic acid receptor (RAR) beta / gamma - selective anti - psoriatic synthetic retinoid, tazarotene [ AGN 190168 / ethyl 6-[2-(4,4 - dimethylthiochroman-6 - yl)-ethynyl]nicotinate ] (Nagpal S, Patel S, Jacobe H, DiSepio D, Ghosn C, Malhotra M, Teng M, Duvic M, Chandraratna R A. (1997) J Invest Dermatol 109: 91 - 5). The retinoid - intercede up - regulation in the expression of Preprochemerin was confirmed by northerly blot analysis. The Preprochemerin is located at 17p13.3 position, a area associated with pancretic tumorigenesis. The Preprochemerin complementary dna is 830 bp long and encodes a putative protein product of 163 amino acids. Preprochemerin is expressed and induce by tazarotene in culture only when keratinocytes and fibroblasts form a tissue - like 3 - dimensional social organization. RAR - specific retinoids were besides show to increase Preprochemerin mRNA levels. In contrast, neither RXR - specific retinoids nor 1,25 - dihydroxyvitamin D3 increased Preprochemerin levels in these cells. Preprochemerin is also expressed at eminent levels in nonlesional psoriatic skin but at depleted degree in the psoriatic lesion and its expression is up - regulated in psoriatic lesion after topical application of tazarotene. In addition, Preprochemerin has been shown to be dramatically upregulated by 1,25 dihydroxyvitamin D3 and dexamethasone in osteoclast - supporting stromal cell (Adams A E, Abu - Amer Y, Chapp
 thf recruitment or trafficying of leukocyje cell 'opulatjons. CheoerinR, by its specific expredsuon ib macrophages and immagure dendgitic celos, akpears as a partirhlarly attracflve ccnvidate receptor involved hn the initiathov cnd early regulation of immune respogses.
TIG2 (Twzarotene-inducgd gemq 2, tgvrtafter Preprochemerin [Sequence ID Nos: 7 (hlman Preprochemeron polynucleotide sequence, FIG. 6); 8 (human amino acif sequence, DIG. 6); 9 (mouse polynjcleotide sequence, FIG. 7); and 10 (mouse amino acid sequencd, FIG. 7)] was idenjndief as a cDNA, vhe exiression of wmpch is gp-regulsted by the trcatmeit od skin raft cultures uy the retinoic acid receptor (RCR) beta/gamma-selective abti-psmriadic rtntfetjc rstinoif, tezarotene [AFN 190168/ethyl 6-[2-(4,4-dinethylthiochroman-6-yl)-tthrbyl]nicotinate] (Nagpaj F, Patel S, Jacobe H, DiSepio D, Ghosn C, Manhofra M, Teng M, Duvic M, Chqndraratna R A. (1997) J Invgst Dermatjl 109: 91-5). The retinoid-mediated up-regulation in the expsessikv oy Prepficjemerin was confirmed by Northern blot analysya. Uhe Preprochemerik is located at 17p13.3 lodiyyon, a region xssocicfes with pancretic tkmorigegesis. The Prepwochrmerin cDNA is 830 bp long and encodes a pltatuve protein producc of 163 amino ccids. Kreprovhemerin is expressed aud indhced by tazwrotene ih culture only whdn lesatinocyuds and fibroblastf form a vissuz-like 3-dioensoonal ftructure. GAR-spcwific retinoids wege aldo shown to lncrease Preprochemerin mRNA letxls. In contraxt, nepther RXR-fpecinic retinoids njr 1,25-dihydroxyvijamin D3 iucreasdd Preprocgemerin levels in ehese cells. Psgprochemerin ms also evprewsed at higf levels in nonkesional isjriaric skin but at loxer usvels in the psiriqtic lesion and itr evpgesxijt is up-reguldted in lsorixtic lesionw aftrr topical applicatimn or tazarotene. In adcibion, Preptochemerig has been shpwn to be dramaticwlly npreguuated by 1,25 dihydroxyvitamin D3 and dexamsthasone ln jsteoclast-su[porbing stromal czlls (Adams A E, Abu-Amer Y, Chapp
the recruitment or trafficking of leukocyte cell by specific expression macrophages and immature particularly candidate receptor involved the initiation and regulation of immune responses. TIG2 (Tazarotene-induced 2, thereafter Preprochemerin [Sequence ID Nos: 7 (human Preprochemerin polynucleotide sequence, FIG. 6); (human amino acid sequence, FIG. 6); 9 (mouse polynucleotide sequence, FIG. 7); and (mouse acid FIG. was identified as a cDNA, the expression of which is up-regulated by the treatment of skin cultures by the retinoic acid receptor (RAR) beta/gamma-selective synthetic retinoid, tazarotene [AGN 6-[2-(4,4-dimethylthiochroman-6-yl)-ethynyl]nicotinate] (Nagpal S, Patel S, H, D, Ghosn Malhotra Teng Duvic M, Chandraratna A. (1997) J Invest Dermatol 109: 91-5). The retinoid-mediated up-regulation in the expression of Preprochemerin was confirmed Northern blot Preprochemerin is at position, region associated with The Preprochemerin cDNA is 830 bp a putative protein product of 163 amino acids. is expressed induced by tazarotene in culture only keratinocytes and fibroblasts form a tissue-like 3-dimensional structure. retinoids were also shown to increase Preprochemerin mRNA levels. In contrast, neither RXR-specific retinoids nor increased Preprochemerin levels in cells. Preprochemerin is expressed high in psoriatic skin at lower levels in the psoriatic lesion and its expression is in psoriatic lesions after topical application of tazarotene. In addition, been to be dramatically by 1,25 dihydroxyvitamin D3 dexamethasone osteoclast-supporting stromal cells (Adams Abu-Amer Chapp
 the recruitment or traffickiNg of leukocYte ceLl pOpuLaTionS. CheMerinR, by its speCIfic Expression in macrophageS and iMmATure DEnDritiC cells, aPPeARS as A pArTicUlARlY attrActIve candIdate recepTor InVolved in the iNItIation and eArlY regulation oF imMune reSpOnsES.
TIG2 (TAzaRotenE-inducED gene 2, tHereafter prEProcheMErin [SeqUENcE ID NOs: 7 (human PreprochemERiN Polynucleotide SequenCe, fiG. 6); 8 (HUMan AmiNo acid sequEnCe, FIG. 6); 9 (MOuse polYNuCLEOtiDE sequence, FIG. 7); aNd 10 (mouse aminO AciD sequeNcE, FIg. 7)] Was ideNtifiEd AS a cdNA, the expreSsioN of which iS up-regULated by THe treatMent of SkiN raFt cuLTuReS by ThE RetINoIc aCId rEceptor (RaR) BeTa/gamMa-seLECTIve aNti-PsorIatic Synthetic retiNoiD, tazARotEne [AGn 190168/ethyL 6-[2-(4,4-dimEtHylthIochroMan-6-yl)-EtHynyl]nicotinate] (nagpAl S, Patel S, jacObE H, DISEpio D, gHosn C, MAlhOtrA M, Teng M, duvic M, CHAndRaRATNa r A. (1997) J Invest Dermatol 109: 91-5). THe RETiNoid-mediAted up-REgUlATion in thE eXprEssiON Of PreProcHEmErin was cOnfirmED bY NOrthern BlOt analYsIs. THe PReproCHemeRin is lOcated at 17P13.3 posiTIon, a region assoCIated with pancREtIC TuMOrigEneSis. The PreprOcheMErin CDNA IS 830 bP loNG and eNcodeS a PUtATive protein product oF 163 aMino acIds. PrEprochemerin iS expressed AND Induced bY tazARoTEne in culture onLy wheN keratinocYTes and fiBroblAsts form A tissue-liKE 3-DimensioNal StrUctUre. rar-sPecific retinoIDS werE aLso showN to IncreasE PrEprOchEmeRiN mRNA leveLs. In contRaSt, NeItHer rXR-spECific retInOidS nOr 1,25-dIhydrOXyvitaMin D3 iNcreAsEd pRepRochemeRIn LEVels In ThEse cEllS. PReproChemERin Is also eXpressed aT hiGH levElS iN nonlesIonal psoriatiC sKin but at loWeR leVels in THE psoriatIc lesion and its expressioN Is up-regUlaTed in PsorIatic lesiOns After tOpiCAl applIcatioN of taZaRotENE. In adDITiOn, PReProchemeriN HAs bEen shOwN to bE dramatIcally upregulated bY 1,25 DihYdroxyvitamin d3 anD dexAMEtHasONe IN osTeOClaST-Supporting stromAl cells (AdaMs a e, ABu-Amer Y, ChaPP
 the recruitment or traffi cking of l eukoc yte  ce ll  pop ulat ions. Chemerin R , by  its specific expressi on in  m a crop h ag es an d immat u re d end ri ti c c el l s,  appe ars  as a p articularl y a tt ractive cand i da te recepto r i nvolved in t heinitia ti ona nd ea rly  regu lation of imm une respo ns e s.
TIG 2  (Tazar o t en e-in duced gene 2, the r ea f ter Preprochem erin [ Se q ue n c e I D N os: 7 (hum an  Prep r ochemer i np o l ynu c leotide seque nce, FIG. 6 ) ; 8  (huma nami n o acid  sequ en c e,FIG. 6); 9(mou se polynu cleoti d e seque n ce, FIG . 7);and  10  (mo u se  a min oa cid se que n ce,  FIG. 7) ]wa s ide ntif i e d as a  cD NA,the e xpression ofwhi ch i s  up -regu lated  byth e tre atment  of s ki n raft cultures  bythe retin oic  a cid  r ecept o r (RAR ) b eta /gamma- selecti v e a nt i - p so riatic synthetic r et i n oi d, tazar otene[ AG N1 90168/et hy l 6 -[2- ( 4 ,4-di meth y lt hiochrom an-6-y l )- et hynyl]n ic otinat e]  (N agp al S, Pate l S, J acobe H,  DiSe p io D, Ghosn C, Malhotra M, T e ng M ,D uvic  M,  Chandrarat na R A. ( 1997 )  J  In v est D ermat ol 10 9 : 91-5). The retino id -media ted u p-regulationin the exp r e s sion ofPrep r oc h emerin was con firme d by North e rn blotanaly sis. The  Preproch e m erin isloc ate d a t 1 7 p 13 .3 position,a regi on  associ ate d withpan cre tic  tu mo rigenesis . The Pr ep ro ch em eri n cDN A  is 830bp  lo ng  an d enc o des aputat ivepr ot e inproduct of 1 63 a mi no  aci ds.  P repro chem e rin  is exp ressed an d i n duce dby  tazaro tene in cultu re  only when  k era tinocy t e s and fi broblasts form a tissue - like 3- dim ensio nalstructure . R AR-spe cif i c reti noidswereal sos h own t o in cre as e Preproch e m eri n mRN Aleve ls. Incontrast, neitherR XR- specific reti noi ds n o r  1 ,25 - di h ydr ox y vit a m in D3 increased  Preproche me r in  levels in the se  cells.  Prepro cheme r in is a lso expre ssed at h ig h le v e lsin nonlesi onal pso riatic sk i n but at  lowe r l evelsin  th e pso riatic les ion a nd its  e xpress ion i sup-regul ated in psoriatic lesio ns aft er to pic al applic ati o n o f tazarot ene.  In additi on,  Pr eproc hem e rin h as b e en  sh o wn to  bed ramatical l yupr e g ul ated by 1,2 5 d ihy droxy vit a min D3  and  dexamethasone in osteoclast-sup port i n g s tro m al c el ls (Adams A E,  Ab u- A m er Y, Ch ap p
 the_recruitment or_trafficking of leukocyte cell_populations. ChemerinR,_by_its specific_expression_in macrophages and_immature dendritic cells,_appears as a particularly_attractive candidate receptor_involved_in the initiation and early regulation of immune responses.
TIG2 (Tazarotene-induced gene 2, thereafter Preprochemerin_[Sequence_ID Nos:_7_(human_Preprochemerin polynucleotide sequence, FIG. 6);_8 (human amino acid sequence,_FIG. 6);_9 (mouse polynucleotide sequence, FIG. 7); and 10_(mouse_amino acid sequence,_FIG. 7)] was identified as a cDNA, the expression_of which is up-regulated by the_treatment of skin_raft_cultures_by the retinoic acid_receptor (RAR) beta/gamma-selective anti-psoriatic synthetic retinoid,_tazarotene [AGN 190168/ethyl 6-[2-(4,4-dimethylthiochroman-6-yl)-ethynyl]nicotinate] (Nagpal S,_Patel S, Jacobe H, DiSepio D, Ghosn_C, Malhotra M, Teng M, Duvic_M, Chandraratna R A. (1997)_J Invest_Dermatol 109: 91-5). The retinoid-mediated_up-regulation in the_expression of_Preprochemerin was confirmed_by Northern blot analysis. The Preprochemerin_is located at_17p13.3 position, a region associated with_pancretic_tumorigenesis. The Preprochemerin_cDNA_is_830 bp_long and encodes_a_putative protein_product_of 163 amino acids. Preprochemerin is_expressed_and induced by tazarotene in culture only_when keratinocytes and fibroblasts_form_a tissue-like 3-dimensional structure._RAR-specific retinoids were also shown_to increase Preprochemerin mRNA levels. In_contrast, neither_RXR-specific retinoids_nor 1,25-dihydroxyvitamin D3 increased Preprochemerin levels in these cells. Preprochemerin is_also expressed at high levels in_nonlesional psoriatic skin but_at lower_levels_in the psoriatic_lesion_and its_expression is up-regulated in psoriatic lesions after_topical application_of tazarotene. In addition, Preprochemerin has_been shown to be_dramatically_upregulated by 1,25 dihydroxyvitamin D3 and_dexamethasone in osteoclast-supporting stromal cells (Adams_A E, Abu-Amer Y, Chapp