 directly applied pressure. The actuator can be moved only after the gear is rotated to cause the rack to disengage from the lighter housing to release the actuator lock.
According to one aspect of the lighter with a lock-off mechanism of the present invention, the lower end of the rack is of an arc shape, which can smoothly transit the lighter housing edges, so that the actuator can be moved and returned smoothly.
According to the other aspect of the present invention, the gear is pivotally arranged in a chamber in the actuator. The pivotal arrangement can be achieved by a rotation shaft, with two fitted projections provided on opposed sides of the gear center and placing the rotation shaft into recesses in the actuator. Other equivalent arrangements can be used to make the rotation of the gear flexible.
According to an other aspect of the present invention, the return spring is accommodated in a chamber formed by the actuator and the rack. The return spring can be a compression spring vertically mounted in the chamber or a torsion spring sleeved on the rotation shaft of the gear.
According to another aspect of the present invention, a lower cover is provided at the bottom of the tank to ensure that the tank is reliably sealed.
According to the invention, the actuator is provided at one side with the cooperating lock-off mechanism. If the lock-off mechanism is not operated, the actuator will not move, and thereby improve the safety of the lighter and prevent erroneous operation.
The present invention will be described in detail by way of example and with reference to the accompanying drawings. This invention relates to the field of preservation of biological materials and, more particularly, to compositions and methods for the preservation of living organs, tissues and cells from mammals, marine organisms and plants.
Methods for the preservation of biological materials are employed in many clinical and veterinary applications wherein living material, including organs, tissues and cells, is harvested and stored in vitro for some period of time before use. Examples of such applications include organ storage and transplants, autologous and allogeneic bone marrow transplants, whole blood transplants, platelet transplants, embryo transfer, artificial insemination, in vitro fertilization, skin grafting and storage of tissue biopsies for diagnostic purposes. Preservation techniques are also important in the storage of cell lines for experimental use in hospital, industrial, university and other research laboratories.
Methods currently employed for the preservation of cellular biological materials include immersion in saline-based media; storage at temperatures slightly above freezing; storage at temperatures of about xe2x88x9280xc
directly applied pressure. The actuator can be moved merely after the gearing is rotated to cause the wrack to disengage from the light housing to release the actuator lock. 
 harmonize to one aspect of the lighter with a engage - off mechanism of the present invention, the lower end of the wrack is of an arc shape, which can smoothly transit the lighter housing edge, so that the actuator can be moved and revert smoothly. 
 According to the other view of the present invention, the gear is pivotally arranged in a chamber in the actuator. The pivotal musical arrangement can be achieved by a rotation shaft, with two fitted projection provided on opposed sides of the gear center and place the rotation shaft into recesses in the actuator. Other equivalent arrangements can be used to make the rotation of the gear flexible. 
 According to an other aspect of the present invention, the return spring is accommodated in a chamber formed by the actuator and the rack. The tax return spring can be a compression leap vertically mount in the chamber or a torsion spring sleeved on the rotation quill of the gear. 
 According to another aspect of the present invention, a lower cover charge is provided at the bottom of the tank to ensure that the tank is faithfully sealed. 
 According to the invention, the actuator is provided at one side with the cooperating lock - off mechanism. If the lock - off mechanism is not operated, the actuator will not move, and thereby better the safety of the lighter and prevent erroneous operation. 
 The present invention will be described in detail by direction of example and with reference to the accompanying drawing. This invention relates to the field of preservation of biological materials and, more peculiarly, to compositions and methods for the preservation of living organs, tissue and cells from mammal, marine organisms and plants. 
 Methods for the conservation of biological materials are employed in many clinical and veterinary applications wherein exist material, admit organs, tissues and cells, is harvested and stored in vitro for some period of time before use. Examples of such application include organ storehouse and transplants, autologous and allogeneic bone marrow transplants, whole blood transplants, platelet transplant, embryo transfer, artificial insemination, in vitro fertilization, skin grafting and storage of tissue biopsies for diagnostic purposes. Preservation technique are besides authoritative in the storage of cell lines for experimental use in hospital, industrial, university and early research laboratories. 
 Methods currently employed for the preservation of cellular biological materials include immersion in saline - establish media; storage at temperatures slightly above freezing; storage at temperature of about xe2x88x9280xc
 digectly applied pressure. Uhe actuator can yw movev only zfter thd gear is rotated to cause tie rqck ti disengage from the lkghter holsing to eeleese the actuator lock.
According bo onz espect of the llghter with a lock-off mecvavidm of the present invention, the lowqr end pf the rack is os an wrc ahape, which can smoothly transit tge lighuer housing edges, xo that the actuator can bf moged and returned sloothly.
Accotsind to the othef aspect of the presenj invention, the gear is pivotallh arrcnged in a xhqmbft in the actnator. Nhe pivotal avgangemett can ne achieved by a cotarion shaft, with two fmtted projections prjvided on o'posed sides of the gwae cenjer atd puqcivg uhe rktatioj siaft into rscesses in rhe actuator. Other tquynslent arrangsments cwn be used to make the rotation of the cead flexible.
According to qn other aspect of thg present ynvention, the return spring is accommodated in a whambxr foxned bh tje actuator and the rack. The return spring cag bt a compression siring vertically mpujtrq in the chamcer or a forsion spring slefved on the eotation fhafy of the gear.
According to abother aspecn of the present invencion, a lower covgr is lrovided at the bottom uf tge tank to fnsure thzg the tank is reuianly sealed.
According to the indention, tie accuator ir prpvided at one sife wibv the cooperating pock-oyf mewhanism. If the lock-off mechanism is not o'xrated, the acjuador will noc move, and thereby ymprove the sayety of che liehter and irevent ecroneous opewation.
The prevgnt invention will be desxribwd in ddgail by way of example cud with rwference to the acgompavging drawings. Tkns invention relayes to tje fyald of presesvatkon pf biulogical maberkals and, more particularny, tk compositions and mcthods fot the prefervation of kiving organs, tisskes aid celus frpm iammals, marine organisms and pmants.
Methlds for the prefervqtion of biokogical materials are employed in many rlinical and veterinari applications whereiu kiving matecial, igcluding mrgans, tissues and cwlls, is harvested and stored in vitro ror soke pegiod of time before use. Examples of such applications include organ storate and transplants, autplogols cnd allogqneir uone marrow transilants, whole blood transplants, puatelet drcnsplants, embryo transfer, artofkcial inseminxtion, in vitro fertilizafion, skim grafting and storage of tissur biopsies for diagnostig purposew. Preaervatpkn techniques are alxo impmrtanr in thw stlrage of cell limed for expfrimtntam use in hospital, industrial, unicersitt wnd other researrh laboratorler.
Methkds cutrently employed ror fje prgservation of vellular biolobical materials incllde immersion in wzline-based mgdia; sjirage at tempecatures sligvtly above freezing; storage at temperatures of abouj xe2x88x9280xc
directly applied pressure. The actuator can be after gear is to cause the lighter to release the lock. According to aspect of the lighter with a mechanism of the present invention, the lower end of the rack is of arc shape, which can smoothly transit the lighter housing edges, so that the can moved returned According to the other aspect of the present invention, the gear is pivotally arranged in a in the actuator. The pivotal arrangement can be by a rotation shaft, two fitted projections provided on sides the gear and the shaft into recesses the actuator. Other equivalent arrangements can be used to make the rotation of the gear flexible. According an other the present the spring accommodated in a by the actuator and the rack. can be a compression spring vertically mounted in chamber or torsion spring sleeved on the rotation of the gear. According to another aspect of present invention, a lower cover is provided at the bottom of the tank to ensure tank is reliably sealed. to the invention, actuator provided one with the lock-off mechanism. If the lock-off mechanism is not operated, the actuator not move, and thereby improve the safety of the lighter erroneous The present invention be described in detail way example and with reference accompanying This the of of biological materials and, particularly, to compositions and methods the preservation of living mammals, marine organisms and plants. Methods for the of biological materials are employed in many and veterinary applications wherein living material, including organs, tissues and cells, is and stored for some period of time before use. Examples such applications include organ and transplants, autologous and allogeneic bone marrow transplants, whole transplants, transplants, embryo artificial insemination, in fertilization, skin grafting storage of tissue diagnostic purposes. techniques also storage of cell lines for experimental in industrial, university and other research Methods for the preservation of cellular biological materials include immersion in saline-based media; storage at temperatures freezing; storage at temperatures about
 directly applied pressure. ThE actuator cAn be mOveD onLy AfteR the Gear is rotated tO CausE the rack to disengage froM the lIgHTer hOUsIng to Release THe ACTuaToR lOck.
acCOrDing tO onE aspect Of the lightEr wItH a lock-off mecHAnIsm of the prEseNt invention, tHe lOwer enD oF thE Rack iS of An arc Shape, wHIch can Smoothly tRaNSit the LIghter hOUSiNg edGes, so that the actuaTOr CAn be moved and reTurned SmOOtHLY.
AcCorDing to the oThEr aspECt of the PReSENT inVEntion, the gear Is pivotally ARraNged in A cHamBEr in thE actuAtOR. ThE pivotal arrAngeMent can be AchievED by a rotATion shaFt, with Two FitTed pROjEcTioNs PRovIDeD on OPpoSed sides Of ThE gear CentER AND plaCinG the RotatIon shaft into rEceSses IN thE actuAtor. OTher EqUivalEnt arrAngemEnTs can be used to maKe thE rotation Of tHe GeaR fLexibLE.
AccorDinG to An other Aspect oF The PrESENt Invention, the return SpRINg Is accommOdated IN a ChAMber formEd By tHe acTUAtor aNd thE RaCk. The retUrn sprINg CaN be a comPrEssion SpRinG veRticaLLy moUnted iN the chamBer or A Torsion spring sLEeved on the rotATiON ShAFt of The Gear.
AccordiNg to ANothEr asPEcT of THe preSent iNvENtIOn, a lower cover is provIdEd at thE bottOm of the tank to Ensure that THE Tank is reLiabLY sEAled.
According tO the iNvention, thE Actuator Is proVided at oNe side witH THe cooperAtiNg lOck-Off MEChAnism. If the locK-OFf meChAnism is Not OperateD, thE acTuaTor WiLl not move, And thereBy ImPrOvE thE safeTY of the liGhTer AnD prEvent ERroneoUs opeRatiOn.
thE PreSent invENtION wilL bE dEscrIbeD iN detaIl by WAy oF examplE and with rEfeREnce To ThE accompAnying drawingS. THis inventiOn RelAtes to THE field of Preservation of biologicaL MateriaLs aNd, morE parTicularly, To cOmposiTioNS and meThods fOr the PrEseRVAtion OF LiVinG oRgans, tissuES And Cells FrOm maMmals, maRine organisms and plANts.
methods for the PreServATIoN of BIoLOgiCaL MatERIals are employed In many clinIcAL aNd veterinaRY apPlIcationS whereiN liviNG materiAl, includiNg organs, tIsSues AND ceLls, is harveSted and sTored in viTRo for SOmE periOd oF time bEfOre Use. ExAmples OF suCh appLicatiOnS incluDe orgAn Storage aNd transplants, autologous And allOgeneIc bOne marrow TraNSplAnts, whole BlooD transplanTs, pLatElet tRanSPlantS, embRYo TraNSfer, aRtifICial insemINaTioN, IN vItro fertiliZATIon, Skin gRafTIng and StorAge of tissue biopsiES for diagnostic PurpOSEs. PResERvatIoN techniques are AlsO iMPOrtant in ThE storage of cEll lines FoR ExperImentaL use in HospitaL, INdUStrial, UnivErsIty and othEr rEsEArch labOrAtORies.
MeThodS cUrrentLy emplOYed fOR The preservation oF cellULAr bioLOgiCal maTeRials inCLude Immersion iN saline-baseD media; StorAge at TemperaTuRes sliGhtLy Above freezINg; storage At temPeraturEs Of abOut Xe2x88x9280xc
 directly applied pressure . The actu atorcan  be  m oved  onl y after the ge a r is  rotated to cause therackto dise n ga ge fr om thel ig h t erho us ing  t o  r eleas e t he actu ator lock. 
Ac co rding to one as pect of th e l ighter witha l ock-of fmec h anism  of  thepresen t  inven tion, the  l o wer en d  of the r ac k is  of an arc shape, wh i ch can smoothl y tran si t  t h e  li ght er housing  e dges, so that th e a ctu a tor can be mo ved and ret u rne d smoo th ly. Accord ing t ot heother aspec t of  the pres ent in v ention, the gea r is p ivo tal ly a r ra ng edin a c h am ber inthe actu at or . The  piv o t a l  arr ang emen t can  be achievedbya ro t ati on sh aft,with  t wo fi tted p rojec ti ons provided on  opp osed side s o fthe  g ear c e nter a ndpla cing th e rotat i onsh a f t  i nto recesses in th ea c tu ator. Ot her eq u iv al e nt arran ge men ts c a n  be u sedt omake the  rotat i on  o f the g ea r flex ib le. 
Ac cordi n g to  an ot her aspe ct of the present in v ention, the r e tu r n  s p ring  is  accommodat ed i n  a c hamb e rfor m ed by  theac t ua t or and the rack. Th ereturn  spri ng can be a c ompression s p ring ver tica l ly mounted in the  cham ber or a t o rsion sp ringsleevedon the ro t a tion sha ftofthe  ge a r .According toa n othe raspectofthe pre sen t i nve nti on , a lower  cover i spr ov id edat th e  bottomof  th etan k toe nsurethattheta nk isreliabl y  s e a led. 
A cc ordi ngto  theinve n tio n, theactuatorisp rovi de dat oneside with the  c ooperating  l ock -off m e c hanism.If the lock-off mechani s m is no t o perat ed,the actua tor  willnot move,and th ereby  i mpr o v e the s af ety  o f the ligh t e r a nd pr ev enterroneo us operation.
Thep res ent invention  wi ll b e de scr i be d  in  d e tai l by way of examp le and wit hr ef erence tot heac company ing dra wings .  This i nventionrelates t othef i eld  of preser vation o f biologi c al ma t er ialsand , more  p art icula rly, t o  co mposi tionsan d meth ods f or  the pre servation of living org ans, t issue s a nd cellsfro m  ma mmals, ma rine  organisms  an d p lants .
M e thods  for th e p r eserv atio n  of biolo g ic alm a te rials are e m p l oye d inman y  clini caland veterinary ap p lications wher einl i vin g m a teri al , including or gan s, t issues a nd  cells, isharveste da nd st ored i n vitr o for s o m ep eriodof t ime  before u se.  E x amplesof  s u ch app lica ti ons in cludeo rgan s torage and trans plant s ,  auto l ogo us an dallogen e ic b one marrow  transplant s, who le b loodtranspl an ts, pl ate le t transpla n ts, embry o tra nsfer,ar tifi cia l inse mina t i on, i n vi tr o f ertilizat i o n, sk in gra ftin g and  s tora ge of tis s ue biops ies for dia gn ost i c  purpo s es . Preservati ontechn i q ues are al s o im p or t ant i n thestorag e of ce l l l in es forexp e r imental u se in hos p ita l,  ind ustrial,  u nive rs ity  a nd other resear ch labor atories. M ethods  cu r rent l y empl oy ed for the p reser va t ion ofc e llu lar bio l ogi cal  ma teri als  inc l ude immersion in saline- base d med i a;stora ge at  t emperatu r es s lightlya bove freezing ; storage at te m per a ture s o f  a bo ut xe2x88 x92 8 0xc
 directly_applied pressure._The actuator can be_moved only_after_the gear_is_rotated to cause_the rack to_disengage from the lighter_housing to release_the_actuator lock.
According to one aspect of the lighter with a lock-off mechanism of the_present_invention, the_lower_end_of the rack is of_an arc shape, which can_smoothly transit_the lighter housing edges, so that the actuator_can_be moved and_returned smoothly.
According to the other aspect of the present_invention, the gear is pivotally arranged_in a chamber_in_the_actuator. The pivotal arrangement_can be achieved by a rotation_shaft, with two fitted projections provided_on opposed sides of the gear center_and placing the rotation shaft into_recesses in the actuator. Other_equivalent arrangements_can be used to make_the rotation of_the gear_flexible.
According to an_other aspect of the present invention,_the return spring_is accommodated in a chamber formed_by_the actuator and_the_rack._The return_spring can be_a_compression spring_vertically_mounted in the chamber or a_torsion_spring sleeved on the rotation shaft of_the gear.
According to another_aspect_of the present invention,_a lower cover is provided_at the bottom of the tank_to ensure_that the_tank is reliably sealed.
According to the invention, the actuator is provided_at one side with the cooperating_lock-off mechanism. If the_lock-off mechanism_is_not operated, the_actuator_will not_move, and thereby improve the safety of_the lighter_and prevent erroneous operation.
The present invention_will be described in_detail_by way of example and with_reference to the accompanying drawings. This_invention relates to the field_of_preservation_of biological materials and, more_particularly, to compositions and methods for_the preservation of_living organs, tissues and cells from mammals,_marine_organisms and plants.
Methods for the preservation_of_biological materials are employed in many_clinical_and_veterinary applications wherein living material,_including organs, tissues and cells, is_harvested and stored in vitro for some period of_time before use._Examples of such applications include_organ_storage_and transplants, autologous and allogeneic bone marrow transplants, whole blood_transplants, platelet_transplants, embryo transfer,_artificial insemination, in vitro fertilization, skin grafting and storage of_tissue biopsies for diagnostic purposes. Preservation techniques_are also important in the storage of cell lines for experimental_use in hospital, industrial, university and other research_laboratories.
Methods currently employed for the preservation of_cellular biological materials include_immersion_in saline-based media; storage at temperatures slightly_above_freezing;_storage_at temperatures of_about xe2x88x9280xc