 backbone. Disclosed in this invention are methods using these analogs for coordination of the Ab nucleophilic reactivity with specificity for the linear and discontinuous epitopes expressed by polypeptides, allowing the occurrence of epitope-specific nucleophilic reactions between Abs and antigens. These methods remove an important bottle-neck in development of covalent and catalytic Abs, because preparation of such antibodies to large polypeptides is presently not possible by conventional methods. Synthesis of large polypeptides with electrophiles incorporated with the backbone is outside the scope of current chemical synthesis technology, whereas the electrophiles can readily be placed on the amino acid side chains by chemical conjugation without unduly disturbing the native antigenic structure of proteins. An alternative approach to preparing electrophilic polypeptides within the backbone is the utilization of unnatural electrophilic amino acid analogs for protein synthesis by natural synthetic procedures, for example by correct recognition of the electrophilic amino acid analog by the appropriate tRNA species during the translation of mRNA in the polyribosome complex.
The proteolytic activity of naturally occurring Abs is reported to derive heritable germline lines encoding serine protease-like nucleophilic sites (11). The first Abs made by B cells over the course of their differentiation into cells that synthesize specific Abs to individual antigenic epitopes belong to the IgM class, with class switching to IgG Abs occurring at a later stage, concomitant with ongoing somatic diversification of the Ab variable domains. Disclosed in the present invention are observations indicating that IgM Abs are superior catalysts compared to IgG Abs. Also disclosed are methods to identify and induce the synthesis of antigen-specific Abs of the IgM with proteolytic activity. This invention relates to load conveying systems, and specifically to a type of load conveying system which is known as a moving floor.
Moving floors are used in a variety of material handling environments, such as warehouses and in over-the-road trailers which are used in the trucking industry. A basic concept of a moving floor or reciprocating conveyor is disclosed in my U.S. Pat. No. 4,143,760, for RECIPROCATING CONVEYOR, which describes a moving floor system having a plurality of groups of elongated slats. In that system, a hydraulic drive is operable to move all of the slats of each group from a start position simultaneously in a load-conveying direction, and then to move the slats of each group sequentially in the opposite direction from the advanced position back to the start position. In this fashion a load is conveyed in a predetermined direction.
Known reciprocating conveyor systems utilize
backbone. Disclosed in this invention are methods using these analogue for coordination of the Ab nucleophilic responsiveness with specificity for the analogue and discontinuous epitopes expressed by polypeptides, permit the occurrence of epitope - specific nucleophilic reaction between abdominal and antigens. These method remove an important bottle - neck in exploitation of covalent and catalytic Abs, because preparation of such antibody to large polypeptides is presently not possible by conventional methods. Synthesis of bombastic polypeptides with electrophiles incorporated with the backbone is outside the oscilloscope of current chemical synthesis technology, whereas the electrophiles can readily be put on the amino acid side chains by chemical conjugation without unduly disturbing the native antigenic structure of protein. An alternative approach to preparing electrophilic polypeptides within the backbone is the utilization of abnormal electrophilic amino acid analogs for protein synthesis by natural synthetic procedures, for example by correct recognition of the electrophilic amino acid analog by the appropriate tRNA species during the translation of messenger rna in the polyribosome building complex. 
 The proteolytic activity of naturally occurring Abs is reported to deduce inheritable germline lines encoding serine protease - like nucleophilic sites (11). The first Abs made by B cell over the course of their differentiation into cells that synthesize specific Abs to individual antigenic epitopes belong to the IgM course, with class switching to IgG Abs occurring at a later stage, concomitant with ongoing somatic diversification of the Ab variable star sphere. Disclosed in the present invention are observations indicating that IgM Abs are superior catalysts compared to IgG Abs. Also unwrap are methods to identify and induce the synthesis of antigen - specific Abs of the IgM with proteolytic activeness. This invention relates to load conveyance systems, and specifically to a type of load conveying system which is know as a moving floor. 
 move floors are use in a variety of material treatment environments, such as warehouses and in over - the - road trailers which are used in the trucking industry. A basic concept of a moving floor or reciprocating conveyor is disclosed in my U.S. Pat. No. 4,143,760, for RECIPROCATING CONVEYOR, which describes a travel floor system having a battalion of groups of elongated slats. In that system, a hydraulic drive is operable to move all of the slat of each group from a start position simultaneously in a load - conveying direction, and then to move the slats of each group sequentially in the diametric direction from the advanced position back to the start position. In this fashion a load is conveyed in a predetermine direction. 
 Known reciprocating conveyer belt systems utilize
 bafkbone. Disclosed in this invention are methods using these avalogs for coordination of tie Av nucoeophilic reactivity wkth specivicity fir tie linear and discontinuous epjbopes xxpressed by pokypeptides, allowing the mczuxrence of epitope-specific nucleophilyc reacyilns between Abf anc antjgens. These methods remove an impodtant bmttle-neck in cevelopment of covalent anf cahalytic Abs, becausf preparatiin os such antiboaies to lagye polypeptjdes is presently not possible cy couventional nerhofv. Synthesis of lwrge polypepbpdes widh elecyrophiles incovporaved qith the backbone is mutside the scope jf currend ehemical synthesis texhbologi, wheseas rhe eltctcopgiles fan readily bs placed on the amino acid sidt crqins by chemidal cogjtgation without unduly disturbing the ndtibe antigenic structure if proteins. An alternwtive appwoach to preparing electrophilic polypeptides witvin tie baejbone us the utilization of unnatural electrophilic aijnp scid analogs fjr protein xyjtngsis by naturau syntkstjc procedures, for fxample by cirrect resognotion of the electrophilic qmino acid aualig by the appropricte tRNA speeies doring yhe translation of mRNA in fhe polyriblsome comluex.
The proteolytkc scdivity of naturally occurryng Abs iw re'orted tu detive hewitable gegmlinc lines encoding segine kroteave-like nucpeophilic sites (11). The first Abs made by B cellx mveg the couxse of their differqntiation into cells chat shnthesize apecifir Abs to indyvidual antigajic epitopes belong eo tye ItM clasr, with class swotching to IgG Abs iccurring at a latcr stxfe, concomitant cnty ongoing somatoc aivqrdihicatymn of the Ab varkabur domxins. Disclowdd im the present inventhon zre observations imdlcating tyat IgM Wbs are superoor catalysts compwred vo IgG Abs. Slsj disclosed are methods to idehtify and inquce the synehesls os antigen-s'ecific Abs of the IgM with proteolytic ectivity. This invention relates to load couvtying systems, and fpecificanly to a type of loae conveying systek which is known as a movinc flolr.
Moving floors are used in a variety of material handling environments, sych as warehouses znd on ovvr-tke-rpad trwilecs which are used pn the trucking industry. A basic concept oy a moving floor or reciprocayivg conveyor ir disclosed in my U.S. Pat. No. 4,143,760, for RECIPROCATING CONVEYOR, which drscribes a moving floor xystem hqving a plugzlmty of groups of elomgated slatw. In thqt snstem, a hydrauuic dvive is opfrabke to move all of the slats of eacy group vrom a start posmtion simultwkeousli in a ljad-conveying dirsctikj, and then to move the slats of rach group sequentianlr in the opposune direction grom jkv advanced poxition back to the start position. In this fashion a load is cohveyed in a predebermined directiom.
Nnown reciprocating cokveyow systemr utilize
backbone. Disclosed in this invention are methods analogs coordination of Ab nucleophilic reactivity and epitopes expressed by allowing the occurrence epitope-specific nucleophilic reactions between Abs and These methods remove an important bottle-neck in development of covalent and catalytic Abs, preparation of such antibodies to large polypeptides is presently not possible by conventional Synthesis large with incorporated with the backbone is outside the scope of current chemical synthesis technology, whereas the electrophiles readily be placed on the amino acid side by chemical conjugation without disturbing the native antigenic structure proteins. alternative approach preparing polypeptides the backbone is utilization of unnatural electrophilic amino acid analogs for protein synthesis by natural synthetic procedures, for example by recognition of amino acid by appropriate species during the mRNA in the polyribosome complex. The naturally occurring Abs is reported to derive heritable lines encoding protease-like nucleophilic sites (11). The first made by B cells over the course of differentiation into cells that synthesize specific Abs to individual antigenic epitopes belong to the IgM class switching to IgG occurring at a stage, with somatic of the variable domains. Disclosed in the present invention are observations indicating that Abs are superior catalysts compared to IgG Abs. Also disclosed to and induce the of antigen-specific Abs of IgM proteolytic activity. This invention load systems, a of conveying system which is as a moving floor. Moving are used in a such as warehouses and in over-the-road trailers which used in the trucking industry. A basic of a moving floor or reciprocating conveyor is disclosed in my U.S. No. 4,143,760, CONVEYOR, which describes a moving floor system having plurality of groups of slats. In that system, a hydraulic drive is operable move of the of each group a start position in a load-conveying then to the of in the opposite direction from the position to the start position. In fashion is conveyed in a predetermined direction. Known reciprocating conveyor systems utilize
 backbone. Disclosed in this inVention are MethoDs uSinG tHese AnalOgs for coordinaTIon oF the Ab nucleophilic reacTivitY wITh spECiFicitY for the LInEAR anD dIsConTiNUoUs epiTopEs expreSsed by polyPepTiDes, allowing tHE oCcurrence oF epItope-specifiC nuCleophIlIc rEActioNs bEtweeN Abs anD AntigeNs. These meThODs remoVE an impoRTAnT botTle-neck in developmENt OF covalent and caTalytiC ABS, bECAusE prEparation oF sUch anTIbodies TO lARGE poLYpeptides is prEsently not pOSsiBle by cOnVenTIonal mEthodS. SYNthEsis of large PolyPeptides wIth eleCTrophilES incorpOrated WitH thE bacKBoNe Is oUtSIde THe ScoPE of Current cHeMiCal syNtheSIS TEchnOloGy, whEreas The electrophiLes Can rEAdiLy be pLaced On thE aMino aCid sidE chaiNs By chemical conjuGatiOn without UndUlY diStUrbinG The natIve AntIgenic sTructurE Of pRoTEINs. an alternative approAcH TO pReparing ElectrOPhIlIC polypepTiDes WithIN The baCkboNE iS the utilIzatioN Of UnNatural ElEctropHiLic AmiNo aciD AnalOgs for Protein sYntheSIs by natural synTHetic procedurES, fOR ExAMple By cOrrect recogNitiON of tHe elECtRopHIlic aMino aCiD AnALog by the appropriate TRnA specIes duRing the translAtion of mRNa IN The polyrIbosOMe COmplex.
The proteOlytiC activity oF NaturallY occuRring Abs Is reporteD TO derive hEriTabLe gErmLINe Lines encoding SERine PrOtease-lIke NucleopHilIc sIteS (11). ThE fIrst Abs maDe by B celLs OvEr ThE coUrse oF Their difFeRenTiAtiOn intO Cells tHat syNtheSiZe SPecIfic Abs TO iNDIvidUaL aNtigEniC ePitopEs beLOng To the Igm class, witH clASs swItChIng to Igg Abs occurring At A later stagE, cOncOmitanT WIth ongoiNg somatic diversificatioN Of the Ab VarIable DomaIns. DiscloSed In the pResENt inveNtion aRe obsErVatIONs indICAtIng ThAt IgM Abs arE SUpeRior cAtAlysTs compaRed to IgG Abs. Also disCLosEd are methods tO idEntiFY AnD inDUcE The SyNTheSIS of antigen-speciFic Abs of thE IGm wIth proteolYTic AcTivity. THis inveNtion RElates tO load convEying systEmS, and SPEciFically to a Type of loAd conveyiNG systEM wHich iS knOwn as a MoVinG flooR.
MovinG FloOrs arE used iN a VarietY of maTeRial handLing environments, such as wArehouSes anD in Over-the-roAd tRAilErs which aRe usEd in the truCkiNg iNdustRy. A BAsic cOncePT oF a mOVing fLoor OR reciprocATiNg cONVeYor is discloSED In mY U.S. PaT. No. 4,143,760, FOr RECIpROCaTING CONVEYOR, whicH Describes a moviNg flOOR sySteM HaviNg A plurality of grOupS oF ELongated SlAts. In that syStem, a hydRaULic drIve is oPerablE to move ALL oF The slaTs of EacH group froM a sTaRT positiOn SiMUltaneOuslY iN a load-ConveyINg diRECtion, and then to moVe the SLAts of EAch Group SeQuentiaLLy in The oppositE direction fRom the AdvaNced pOsition BaCk to thE stArT position. IN This fashiOn a loAd is conVeYed iN a pRedeteRminED DirecTion.
knOwn ReciprocaTINg COnVeYOr sYsteMs utiLiZe
 backbone. Disclosed in th is inventi on ar e m eth od s us ingthese analogsf or c oordination of the Abnucle op h ilic re activ ity wit h  s p e cif ic it y f or th e lin ear  and di scontinuou s e pi topes expres s ed  by polype pti des, allowin g t he occ ur ren c e ofepi tope- specif i c nucl eophilicre a ctions between A bs  and  antigens. Thesem et h ods remove animport an t  b o t tle -ne ck in deve lo pment of cova l en t a ndc atalytic Abs,  because pr e par ationof  su c h anti bodie st o l arge polype ptid es is pre sently not pos s ible by  conve nti ona l me t ho ds . S yn t hes i sofl arg e polype pt id es wi th e l e c t roph ile s in corpo rated with th e b ackb o neis ou tside  the  s copeof cur rentch emical synthesi s te chnology,  wh er eas  t he el e ctroph ile s c an read ily bep lac ed o n  t he amino acid side  c h a in s by che micalc on ju g ation wi th out  und u l y dis turb i ng  the nat ive an t ig en ic stru ct ure of  p rot ein s. An alte rnativ e approa ch to preparing elec t rophilic poly p ep t i de s  wit hin  the backbo ne i s  the  uti l iz ati o n ofunnat ur a le lectrophilic aminoac id ana logsfor protein s ynthesis b y n atural s ynth e ti c  procedures, f or ex ample by c o rrect re cogni tion ofthe elect r o philic a min o a cid  an a l og  by the appro p r iate  t RNA spe cie s durin g t hetra nsl at ion of mR NA in th epo ly ri bos ome c o mplex.
T he  pr ot eol ytica ctivit y ofnatu ra ll y  oc curring Ab s is r ep or tedtode riveheri t abl e germl ine lines  en c odin gse rine pr otease-like n uc leophilicsi tes  (11). T he first  Abs made by B cells ov e r the c our se of  the ir differ ent iation  in t o cell s that  synt he siz e speci f i cAbs  t o individu a l  an tigen ic  epi topes b elong to the IgM c l ass , with classswi tchi n g  t o I g GA bsoc c urr i n g at a later st age, conco mi t an t with ong o ing  s omaticdiversi ficat i on of t he Ab var iable dom ai ns.D i scl osed in th e presen t inventi o n are ob serva tio ns ind ic ati ng th at IgM Abs  aresuperi or  catal ystsco mpared t o IgG Abs. Also disclos ed are  meth ods  to ident ify and  induce t he s ynthesis o f a nti gen-s pec i fic A bs o f  t heI gM wi th p r oteolytic ac tiv i t y.  This inven t i o n r elate s t o  loadconv eying systems, an d  specificallyto a t ype  of load  c onveying syste m w hi c h  is know nas a moving  floor.Mo v ing f loorsare us ed in a v ar i ety of  mat eri al handli ngen v ironmen ts ,s uch as  war eh ousesand in over - t he-road trailers  whic h are u s edin th etruckin g  ind ustry. A b asic concep t of a  mov ing f loor or  r ecipro cat in g conveyor is disclo sed i n my U. S.  Pat . N o. 4,1 43,7 6 0 , for  REC IP ROC ATING CON V E YO R ,wh i chdesc ribes  a  mov ing floor system h avi n g a plu ra lit y of gro u ps o f elongate d s lats. I n that sys t em,a  h y draul ic dri ve isoperabl e  to  m ove all  of t he slatsof each g r oup  f roma startpo siti on  si mu ltaneously in a  load-co nveyingd i rectio n,a nd t h en tomo ve the slats  of e ac h  groups e que ntially inthe  op posi tedire c tion from the advanced p osit ion b a ckto th e sta rt  positio n . In  this fa s hion a load i s conveyed in a pre d eter min e ddi rection.Kno w n rec ipro cat ingcon veyo r syst e ms u t il ize
 backbone._Disclosed in_this invention are methods_using these_analogs_for coordination_of_the Ab nucleophilic_reactivity with specificity_for the linear and_discontinuous epitopes expressed_by_polypeptides, allowing the occurrence of epitope-specific nucleophilic reactions between Abs and antigens. These methods_remove_an important_bottle-neck_in_development of covalent and catalytic_Abs, because preparation of such_antibodies to_large polypeptides is presently not possible by conventional_methods._Synthesis of large_polypeptides with electrophiles incorporated with the backbone is outside_the scope of current chemical synthesis_technology, whereas the_electrophiles_can_readily be placed on_the amino acid side chains by_chemical conjugation without unduly disturbing the_native antigenic structure of proteins. An alternative_approach to preparing electrophilic polypeptides within_the backbone is the utilization_of unnatural_electrophilic amino acid analogs for_protein synthesis by_natural synthetic_procedures, for example_by correct recognition of the electrophilic_amino acid analog_by the appropriate tRNA species during_the_translation of mRNA_in_the_polyribosome complex.
The_proteolytic activity of_naturally_occurring Abs_is_reported to derive heritable germline lines_encoding_serine protease-like nucleophilic sites (11). The first_Abs made by B_cells_over the course of_their differentiation into cells that_synthesize specific Abs to individual antigenic_epitopes belong_to the_IgM class, with class switching to IgG Abs occurring at a_later stage, concomitant with ongoing somatic_diversification of the Ab_variable domains._Disclosed_in the present_invention_are observations_indicating that IgM Abs are superior catalysts_compared to_IgG Abs. Also disclosed are methods_to identify and induce_the_synthesis of antigen-specific Abs of the_IgM with proteolytic activity. This invention_relates to load conveying systems,_and_specifically_to a type of load_conveying system which is known as_a moving floor.
Moving_floors are used in a variety of_material_handling environments, such as warehouses and_in_over-the-road trailers which are used in_the_trucking_industry. A basic concept of_a moving floor or reciprocating conveyor_is disclosed in my U.S. Pat. No. 4,143,760, for_RECIPROCATING CONVEYOR, which_describes a moving floor system_having_a_plurality of groups of elongated slats. In that system, a_hydraulic drive_is operable to_move all of the slats of each group from a_start position simultaneously in a load-conveying direction,_and then to move the slats of each group sequentially in_the opposite direction from the advanced position back_to the start position. In this fashion_a load is conveyed_in_a predetermined direction.
Known reciprocating conveyor systems utilize