 strand which is then analyzed by MS. Kits for target nucleic acid preparation are also provided.
PCT WO97/33000 discloses methods for detecting mutations in a target nucleic acid by nonrandomly fragmenting the target into a set of single-stranded nonrandom length fragments and determining their masses by MS.
U.S. Pat. No. 5,605,798 describes a fast and highly accurate mass spectrometer-based process for detecting the presence of a particular nucleic acid in a biological sample for diagnostic purposes.
WO 98/21066 describes processes for determining the sequence of a particular target nucleic acid by mass spectrometry. Processes for detecting a target nucleic acid present in a biological sample by PCR amplification and mass spectrometry detection are disclosed, as are methods for detecting a target nucleic acid in a sample by amplifying the target with primers that contain restriction sites and tags, extending and cleaving the amplified nucleic acid, and detecting the presence of extended product, wherein the presence of a DNA fragment of a mass different from wild-type is indicative of a mutation. Methods of sequencing a nucleic acid via mass spectrometry methods are also described.
WO 97/37041, WO 99/31278 and U.S. Pat. No. 5,547,835 describe methods of sequencing nucleic acids using mass spectrometry. U.S. Pat. Nos. 5,622,824, 5,872,003 and 5,691,141 describe methods, systems and kits for exonuclease-mediated mass spectrometric sequencing.
Thus, there is a need for a method for bioagent detection and identification which is both specific and rapid, and in which no nucleic acid sequencing is required. The present invention addresses this need. The present invention relates to a method and apparatus for applying a reclosable fastener strip to the continuous web of film on a form, fill and seal machine. There are two manufacturing methods for forming reclosable packages on form, fill and seal machines. In one method, the reclosable fastener strip extends parallel to the feed direction of the continuous web of film. In the second method, the reclosable fastener strip extends transverse to the feed direction of the continuous web of film. The present invention relates to the method in which the reclosable fastener strip extends transverse to the feed direction of the continuous web of film. It is important in forming rcclosablc packages using this method that the timing of the cross sealing-jaws be coordinated with the position at which the reclosable
strand which is then analyzed by MS. Kits for target nucleic acid preparation are besides provide. 
 PCT WO97/33000 discloses methods for detect mutations in a aim nucleic acid by nonrandomly fragmenting the target into a stage set of single - stranded nonrandom duration fragments and determining their batch by MS. 
 U.S. Pat. No. 5,605,798 describes a fast and highly accurate mass spectrometer - based procedure for detecting the presence of a particular nucleic acid in a biological sample distribution for diagnostic purposes. 
 WO 98/21066 describes process for determining the succession of a particular aim nucleic acid by mass spectrometry. Processes for detecting a prey nucleic acid present in a biological sample by PCR amplification and mass spectrometry detection are disclosed, as are methods for detecting a target nucleic acid in a sample by amplifying the target with primers that contain restriction sites and tags, extending and cleave the amplified nucleic acid, and detecting the bearing of extensive intersection, wherein the presence of a DNA shard of a mass unlike from wild - type is indicative of a mutation. method of sequencing a nucleic acid via mass spectrometry methods are also described. 
 WO 97/37041, WO 99/31278 and U.S. Pat. No. 5,547,835 describe methods of sequencing nucleic acids use batch spectrometry. U.S. Pat. Nos. 5,622,824, 5,872,003 and 5,691,141 describe methods, systems and kits for exonuclease - mediated bulk spectrometric sequencing. 
 Thus, there is a motivation for a method for bioagent detection and identification which is both specific and rapid, and in which no nucleic acid sequencing is want. The present invention addresses this indigence. The present invention relates to a method and apparatus for apply a reclosable fastener strip to the continuous vane of film on a phase, fill and cachet machine. There are two fabrication methods for forming reclosable packages on form, fill and seal machines. In one method, the reclosable fastener strip extends parallel to the feed direction of the continuous web of movie. In the second method, the reclosable fastener strip exsert transverse to the feed direction of the continuous web of film. The present invention relates to the method in which the reclosable fastener strip extend transverse to the feed direction of the continuous web of film. It is important in form rcclosablc software practice this method that the timing of the cross sealing - jaws be coordinated with the position at which the reclosable
 stgand which is then analyeed by MS. Kits fot rarget nuclejc acid oreparation are also providev.
PCT WO97/33000 duscloses methods for ddtecting lutationw in q target nndleic agnd by konraudimly fragmentikg the targat into a set mf snngle-stranded nonrandom length fragmqnts anc fetermining thgir msfses by MS.
U.S. Pat. No. 5,605,798 describes a fast znd higily accurate maxs spectrometer-based proceds flr detecting the pgesence of q pawricular nucldic acid in a biologiczl sample for diagnostic purposds.
WO 98/21066 describes peocfvses for deverminpng the sequekbe of a particilar target nugleic acud by mass spectrometcy. Processes for detgcting a tdryet nucleic acid preswnr in d bimlogkxal sajpke by PCG ajplificatikn and mass spectrometry detecuiog are discloses, as awe methods for detecting a target nucleic acjd in a sample by amplidying the target with primers ehat contain restriction sites and tags, extending and rldavnkn thd alplified nucleic acid, and detecting the presegde on extended produgt, wherein the prexejcr of a DNA fraement of z mass different fgom wilq-type is indicwtivr of a mutation. Methods of wequencing a bucleic acid via mcss spectromztry mgthods are also described.
WO 97/37041, CO 99/31278 ans U.S. Pat. No. 5,547,835 describs methods of sequdncpng tucleic acids using mass s[ectrometcy. U.S. Pat. Nor. 5,622,824, 5,872,003 snd 5,691,141 dqscribe mehhods, systems and kits flr exlngclease-medlated mass spectrometric sequenrmng.
Thus, there iv a need fox a mebhod for bioagegt detection aud identnficatkon which ps both s'ecific and wapid, and in fjich no nuclxic acid fequwncibg is rdduired. The prexent inveuuion addrewses this need. The prgssnt invention rzuqtes to a methoc avd wpiaretus smr applying d rezlorsble wastener stvip to yhe continuous web ox fimm on a form, fill snq seal mqchine. Trere are two kanufacturing methlds fmr horminb rgclosable packages on form, fill and seal maghines. In one metmod, jhe reclosayle fastener strip extends parallel to tie feed direction of thw continuous web of nilm. In the xeconq method, dhe reclosable fasteber strip extends transverse to the fesd diractioj of the continuous web of film. The present invention relates to the methid in xhych the recmosanle fdscenzr stri[ exveids transverse to the feed direction of the contmnuous web oy film. It is important in forkivg rcclosablc packages using this metgod that the timing of the cross sealinb-jaws be coordinated witm the posution at whpdh the reclosable
strand which is then analyzed by MS. target acid preparation also provided. PCT mutations a target nucleic by nonrandomly fragmenting target into a set of single-stranded length fragments and determining their masses by MS. U.S. Pat. No. 5,605,798 describes fast and highly accurate mass spectrometer-based process for detecting the presence of a nucleic in biological for diagnostic purposes. WO 98/21066 describes processes for determining the sequence of a particular target nucleic by mass spectrometry. Processes for detecting a target acid present in a sample by PCR amplification and spectrometry are disclosed, are for a target nucleic in a sample by amplifying the target with primers that contain restriction sites and tags, extending and the amplified and detecting presence extended wherein the presence DNA fragment of a mass different indicative of a mutation. Methods of sequencing a acid via spectrometry methods are also described. WO WO 99/31278 and U.S. Pat. No. 5,547,835 describe of sequencing nucleic acids using mass spectrometry. U.S. Pat. Nos. 5,622,824, 5,872,003 and 5,691,141 describe and kits for exonuclease-mediated spectrometric sequencing. Thus, is need a for bioagent and identification which is both specific and rapid, and in which nucleic acid sequencing is required. The present invention addresses this present relates to a and apparatus for applying reclosable strip to the continuous film a seal There two manufacturing methods for reclosable packages on form, fill seal machines. In one extends parallel to the feed direction of the web of film. In the second method, reclosable fastener strip extends transverse to the feed direction of the continuous of film. invention relates to the method in which the fastener strip extends transverse the feed direction of the continuous web of film. is in forming packages using this that the timing the cross sealing-jaws with the at the
 strand which is then analyzed By MS. Kits foR targEt nUclEiC aciD preParation are alsO ProvIded.
PCT WO97/33000 discloses methOds foR dETectINg MutatIons in a TArGET nuClEiC acId BY nOnranDomLy fragmEnting the tArgEt Into a set of siNGlE-stranded nOnrAndom length fRagMents aNd DetERminiNg tHeir mAsses bY mS.
U.S. PaT. No. 5,605,798 descriBeS A fast aND highly ACCuRate Mass spectrometer-bASeD Process for deteCting tHe PReSENce Of a Particular NuCleic ACid in a bIOlOGICal SAmple for diagnOstic purposES.
WO 98/21066 DescriBeS prOCesses For deTeRMinIng the sequeNce oF a particuLar tarGEt nucleIC acid by Mass spEctRomEtry. pRoCeSseS fOR deTEcTinG A taRget nuclEiC aCid prEsenT IN A BiolOgiCal sAmple By PCR amplificAtiOn anD MasS specTromeTry dEtEctioN are diSclosEd, As are methods for DeteCting a tarGet NuCleIc Acid iN A samplE by AmpLifying The targET wiTh PRIMeRs that contain restrIcTIOn Sites and Tags, exTEnDiNG and cleaViNg tHe amPLIfied NuclEIc Acid, and dEtectiNG tHe PresencE oF extenDeD prOduCt, wheREin tHe presEnce of a DnA fraGMent of a mass difFErent from wild-TYpE IS iNDicaTivE of a mutatioN. MetHOds oF seqUEnCinG A nuclEic acId VIa MAss spectrometry methOdS are alSo desCribed.
WO 97/37041, WO 99/31278 and u.S. Pat. No. 5,547,835 desCRIBe methodS of sEQuENcing nucleic acIds usIng mass speCTrometry. u.S. Pat. nos. 5,622,824, 5,872,003 and 5,691,141 deScribe metHODs, systemS anD kiTs fOr eXONuClease-mediateD MAss sPeCtrometRic SequencIng.
thuS, thEre Is A need for a Method foR bIoAgEnT deTectiON and idenTiFicAtIon Which IS both sPecifIc anD rApID, anD in whicH No NUCleiC aCiD seqUenCiNg is rEquiREd. THe preseNt inventiOn aDDresSeS tHis need. the present invEnTion relateS tO a mEthod aND ApparatuS for applying a reclosable FAstener StrIp to tHe coNtinuous wEb oF film oN a fORm, fill And seaL machInE. ThERE are tWO MaNufAcTuring methODS foR formInG recLosable Packages on form, fill ANd sEal machines. In One MethOD, ThE reCLoSAblE fASteNER strip extends paRallel to thE fEEd Direction oF The CoNtinuouS web of fIlm. In THe seconD method, thE reclosabLe FastENEr sTrip extendS transveRse to the fEEd dirECtIon of The ContinUoUs wEb of fIlm. The PResEnt inVentioN rElates To the MeThod in whIch the reclosable fasteneR strip ExtenDs tRansverse To tHE feEd directiOn of The continuOus Web Of filM. It IS impoRtanT In ForMIng rcClosABlc packagES uSinG THiS method that THE TimIng of The CRoss seAlinG-jaws be coordinateD With the positioN at wHICh tHe rEClosAbLe
 strand which is then anal yzed by MS . Kit s f orta rget  nuc leic acid prep a rati on are also provided.PCT W O9 7 /330 0 0discl oses me t ho d s  fo rde tec ti n gmutat ion s in atarget nuc lei cacid by nonr a nd omly fragm ent ing the targ etinto a  s eto f sin gle -stra nded n o nrando m lengthfr a gments and det e r mi ning  their masses byM S. U.S. Pat. No.5,605, 79 8  d e s cri bes  a fast an dhighl y  accura t em a s s s p ectrometer-ba sed process for  detec ti ngt he pre sence  o f  aparticularnucl eic acidin a b i ologica l  sample  for d iag nos ticp ur po ses .W O 9 8 /2 106 6  de scribespr oc esses  for d e t ermi nin g th e seq uence of a pa rti cula r  ta rgetnucle ic a ci d bymass s pectr om etry. Processes  for  detectin g a  t arg et  nucl e ic aci d p res ent ina biolo g ica ls a m pl e by PCR amplifica ti o n  a nd massspectr o me tr y  detecti on  ar e di s c losed , as ar e method s ford et ec ting ata rget n uc lei c a cid i n  a s ampleby ampli fying the target wit h  primers that co n t ai n  res tri ction sites  and tags , ex t en din g  andcleav in g  t h e amplified nucleic  a cid, a nd de tecting the p resence of e x tended p rodu c t, wherein the pr esenc e of a DNA fragment  of a  mass di fferent f r o m wild-t ype  is  in dic a t iv e of a mutati o n . Me th ods ofseq uencing  anuc lei c a ci d via mas s spectr om et ry  m eth ods a r e also d es cri be d.WO 97 / 37041,  WO 9 9/31 27 8a ndU.S. Pa t .N o . 5, 54 7, 835des cr ibe m etho d s o f seque ncing nuc lei c  aci ds  u sing ma ss spectromet ry . U.S. Pat .Nos . 5,62 2 , 824, 5,8 72,003 and 5,691,141 de s cribe m eth ods,syst ems and k its  for e xon u clease -media ted m as s s p e ctrom e t ri c s eq uencing.
T h u s,there  i s aneed fo r a method for bio a gen t detection a ndiden t i fi cat i on whi ch isb o th specific and  rapid, an di nwhich no n u cle ic  acid s equenci ng is require d. The pr esent inv en tion a ddr esses this  need. T he presen t  inve n ti on re lat es toamet hod a nd app a rat us fo r appl yi ng a r eclos ab le faste ner strip to the contin uous w eb of  fi lm on a f orm ,  fi ll and se al m achine. Th ere  ar e two  ma n ufact urin g  m eth o ds fo r fo r ming recl o sa ble p ac kages on fo r m ,  fi ll an d s e al mac hine s. In one method, the reclosable  fas t e ner  st r ip e xt ends paralleltoth e feed dir ec tion of the  continu ou s  webof fil m. Inthe sec o n dm ethod,  the  re closablefas te n er stri pex t ends t rans ve rse to  the f e ed d i r ection of the co ntinu o u s web offilm.  T he pres e nt i nvention r elates to t he met hodin wh ich the  r eclosa ble  f astener st r ip extend s tra nsverse  t o th e f eed di rect i o n oftheco nti nuous web o ff il m. Itis i mport an t in  formingr cclosabl c p a ckagesus ing t his me t ho d that the t imi ng of t he cross s e alin g -j a ws be  coord inated  with t h e p os ition a t w h i ch the re closable
 strand_which is_then analyzed by MS._Kits for_target_nucleic acid_preparation_are also provided.
PCT_WO97/33000 discloses methods_for detecting mutations in_a target nucleic_acid_by nonrandomly fragmenting the target into a set of single-stranded nonrandom length fragments and_determining_their masses_by_MS.
U.S._Pat. No. 5,605,798 describes a_fast and highly accurate mass_spectrometer-based process_for detecting the presence of a particular nucleic_acid_in a biological_sample for diagnostic purposes.
WO 98/21066 describes processes for determining_the sequence of a particular target_nucleic acid by_mass_spectrometry._Processes for detecting a_target nucleic acid present in a_biological sample by PCR amplification and_mass spectrometry detection are disclosed, as are_methods for detecting a target nucleic_acid in a sample by_amplifying the_target with primers that contain_restriction sites and_tags, extending_and cleaving the_amplified nucleic acid, and detecting the_presence of extended_product, wherein the presence of a_DNA_fragment of a_mass_different_from wild-type_is indicative of_a_mutation. Methods_of_sequencing a nucleic acid via mass_spectrometry_methods are also described.
WO 97/37041, WO 99/31278_and U.S. Pat. No._5,547,835_describe methods of sequencing_nucleic acids using mass spectrometry._U.S. Pat. Nos. 5,622,824, 5,872,003 and_5,691,141 describe_methods, systems_and kits for exonuclease-mediated mass spectrometric sequencing.
Thus, there is a need_for a method for bioagent detection_and identification which is_both specific_and_rapid, and in_which_no nucleic_acid sequencing is required. The present invention_addresses this_need. The present invention relates to_a method and apparatus_for_applying a reclosable fastener strip to_the continuous web of film on_a form, fill and seal_machine._There_are two manufacturing methods for_forming reclosable packages on form, fill_and seal machines._In one method, the reclosable fastener strip_extends_parallel to the feed direction of_the_continuous web of film. In the_second_method,_the reclosable fastener strip extends_transverse to the feed direction of_the continuous web of film. The present invention relates_to the method_in which the reclosable fastener_strip_extends_transverse to the feed direction of the continuous web of_film. It_is important in_forming rcclosablc packages using this method that the timing of_the cross sealing-jaws be coordinated with the_position at which the reclosable