d.
The term xe2x80x9cpromoterxe2x80x9d is used herein for its art-recognized meaning to denote a portion of a gene containing DNA sequences that provide for the binding of RNA polymerase and initiation of transcription. Promoter sequences are commonly, but not always, found in the 5xe2x80x2 non-coding regions of genes. Sequences within promoters that function in the initiation of transcription are often characterized by consensus nucleotide sequences. These promoter elements include RNA polymerase binding sites, TATA sequences, and transcription factor binding sites. See, in general, Watson et al., eds., Molecular Biology of the Gene, 4th ed., The Benjamin/Cummings Publishing Company, Inc., Menlo Park, Calif., 1987.
A xe2x80x9cpro sequencexe2x80x9d is a DNA sequence that commonly occurs immediately 5xe2x80x2 to the mature coding sequence of a gene encoding a secretory protein.
The pro sequence encodes a pro peptide that serves as a cis-acting chaperone as the protein moves through the secretory pathway.
A xe2x80x9cproteinxe2x80x9d is a macromolecule comprising one or more polypeptide chains. A protein may also comprise non-peptidic components, such as carbohydrate groups. Carbohydrates and other non-peptidic substituents may be added to a protein by the cell in which the protein is produced, and will vary with the type of cell. Proteins are commonly defined in terms of their amino acid backbone structures; substituents such as carbohydrate groups are generally not specified, but may be present nonetheless.
The term xe2x80x9csecretory signal sequencexe2x80x9d denotes a DNA sequence that encodes a polypeptide (a xe2x80x9csecretory peptidexe2x80x9d) that, as a component of a larger polypeptide, directs the larger polypeptide through a secretory pathway of a cell in which it is synthesized. The larger polypeptide is commonly cleaved to remove the secretory peptide during transit through the secretory pathway. A secretory peptide and a pro peptide may be collectively referred to as a pre-pro peptide.
All references cited herein are incorporated by reference in their entirety.
The present invention provides isolated DNA molecules comprising a Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene promoter. The invention also provides isolated DNA molecules comprising a P. methanolic
d. 
 The term xe2x80x9cpromoterxe2x80x9d is used herein for its art - recognized meaning to announce a parcel of a gene containing DNA sequences that leave for the ski binding of RNA polymerase and initiation of transcription. Promoter succession are normally, but not always, found in the 5xe2x80x2 non - coding regions of genes. Sequences within showman that function in the initiation of recording are frequently characterized by consensus nucleotide sequences. These promoter elements include RNA polymerase bind sites, TATA sequences, and transcription gene binding sites. See, in general, Watson et al. , eds. , Molecular Biology of the Gene, fourth ed. , The Benjamin / Cummings Publishing Company, Inc., Menlo Park, Calif., 1987. 
 A xe2x80x9cpro sequencexe2x80x9d is a deoxyribonucleic acid sequence that commonly occurs immediately 5xe2x80x2 to the mature code sequence of a gene encoding a secretory protein. 
 The pro sequence encodes a pro peptide that serves as a commonwealth of independent states - acting chaperone as the protein moves through the secretory pathway. 
 A xe2x80x9cproteinxe2x80x9d is a macromolecule comprising one or more polypeptide chains. A protein may also comprise non - peptidic components, such as carbohydrate groups. carbohydrate and early non - peptidic substituents may be added to a protein by the cell in which the protein is produced, and will change with the type of cell. Proteins are normally defined in term of their amino acid backbone structures; substituents such as carbohydrate group are generally not specified, but may be present nonetheless. 
 The term xe2x80x9csecretory signal sequencexe2x80x9d denotes a DNA succession that encodes a polypeptide (a xe2x80x9csecretory peptidexe2x80x9d) that, as a component of a larger polypeptide, directs the larger polypeptide through a secretory pathway of a cell in which it is synthesize. The larger polypeptide is commonly cleaved to remove the secretory peptide during transit through the secretory nerve pathway. A secretory peptide and a pro peptide may be collectively referred to as a pre - pro peptide. 
 All references cited herein are incorporated by reference in their entirety. 
 The present invention provides isolated DNA molecules incorporate a Pichia methanolica glyceraldehyde-3 - phosphate dehydrogenase (GAPDH) gene promoter. The invention also supply isolated deoxyribonucleic acid molecules comprising a P. methanolic
d.
Thf term xe2x80x9cpromoterxe2x80x9a is used hereiu for ivs art-rscognizea meaning to denote a portioi of a gebe containing DNA sequdnces than provide for rhe bindinj of RNA polymsvase cnv initiation of transcripdion. Promoter veduznces are commonly, but not always, fotnd in yhf 5xe2x80x2 non-codigg rtgijns kf genes. Sequences within promotera that hunction in the initiation of transcriptiln age often characterlzed by conwenstw nucleotide sequences. These promojer elements include RNA polymerxse bnnding sitew, RATW sequences, end trwnscription nsctor tinding sites. See, in nenerel, Wqtson et al., eds., Molecnlar Biology of the Dene, 4th eg., Che Benjamin/Cummings Puvlishhng Wompxby, Knc., Mxnlk Park, Camif., 1987.
A xe2x80x9dpro sequenxexe2x80x9d is a DNA seauqbce that commknly osctrs immediately 5xe2x80x2 to the mature coditg aequence of a gene encoeing a secretory protgin.
The pro sequence encodes a pro peptide that serves as a wis-acvivg emwowrlne as the protein moves through the secretorr pstmway.
A xe2x80x9cprotelnxe2x80x9d is a macrokopevole comprising one ox mkre polypeptide chwins. A kroteib may alsj cokprise non-peptidic componenrs, such as ccrbihydrate groups. Caxbohydrates cnd otner npn-peptidic substituents may be added tl a protejv by the cell in whpch dhe prottkn is produced, anq will vacy wich the thpe pf celj. Proteins are gmmmonly defined in termd mf their alino acid backbone structures; snustituents suvh as carbohybrate nroups are genewally not specnfied, buc may ce present nonethxless.
The teri xe2x80x9csecretmty signal seqnencexe2x80x9q debotew a DNA requence that rncodes a polypeptiee (a xe2x80x9csecretorn pepjisexe2x80x9d) that, as a xomponent of a kareer pllbpeptyge, directs tve lxrgdt polhpeptidt tmrojgh s secretory pathway mf a cell in which it os synthesuzed. The larger polyprptide is commonly cleated to remofe jhe secretory peptide during trznsit thrlugm the secretowy pqthway. A secxetory peptide and a pro peptide may be rollectively referred jo as a pre-pro peptidg.
Alk referencex citqd herein are incorporated by reference in thelr entirety.
The present invendion orovides isolated DNA molecules comprising a Pichia methanolica glyceraldwhyde-3-piofphate dehysrogrnase (GCPDK) gene [romlver. The invention also provides isolated DNA molxcules com[rnsing a P. methanolic
d. The term xe2x80x9cpromoterxe2x80x9d is used herein art-recognized to denote portion of a provide the binding of polymerase and initiation transcription. Promoter sequences are commonly, but always, found in the 5xe2x80x2 non-coding regions of genes. Sequences within promoters that in the initiation of transcription are often characterized by consensus nucleotide sequences. These elements RNA binding TATA sequences, and transcription factor binding sites. See, in general, Watson et al., eds., Molecular Biology the Gene, 4th ed., The Benjamin/Cummings Publishing Company, Menlo Park, Calif., 1987. xe2x80x9cpro sequencexe2x80x9d is a DNA that occurs immediately to mature sequence of a encoding a secretory protein. The pro sequence encodes a pro peptide that serves as a cis-acting chaperone the protein the secretory A is macromolecule comprising one polypeptide chains. A protein may also such as carbohydrate groups. Carbohydrates and other non-peptidic may be to a protein by the cell which the protein is produced, and will vary the type of cell. Proteins are commonly defined in terms of their amino acid backbone such as carbohydrate groups generally not specified, may present The xe2x80x9csecretory signal denotes a DNA sequence that encodes a polypeptide (a xe2x80x9csecretory peptidexe2x80x9d) as a component of a larger polypeptide, directs the larger a pathway of a in which it is The polypeptide is commonly cleaved the peptide the pathway. secretory peptide and a peptide may be collectively referred as a pre-pro peptide. incorporated by reference in their entirety. The present provides isolated DNA molecules comprising a Pichia glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene promoter. The invention also provides isolated DNA molecules a P.
d.
The term xe2x80x9cpromoterxe2x80x9D is used herEin foR itS arT-rEcogNizeD meaning to denoTE a poRtion of a gene containing dNA seQuENces THaT provIde for tHE bINDinG oF RnA pOlYMeRase aNd iNitiatiOn of transcRipTiOn. Promoter seQUeNces are comMonLy, but not alwaYs, fOund in ThE 5xe2X80X2 non-cOdiNg regIons of GEnes. SeQuences wiThIN promoTErs that FUNcTion In the initiation of TRaNScription are ofTen chaRaCTeRIZed By cOnsensus nuClEotidE SequencES. THESE prOMoter elements Include RNA pOLymErase bInDinG Sites, TaTA seQuENceS, and transcrIptiOn factor bInding SItes. See, IN generaL, WatsoN et Al., eDs., MoLEcUlAr BIoLOgy OF tHe GENe, 4tH ed., The BeNjAmIn/CumMingS pUBLishIng compAny, InC., Menlo Park, CalIf., 1987.
A Xe2x80x9CPro SequeNcexe2X80x9d iS a dNA seQuence That cOmMonly occurs immeDiatEly 5xe2x80x2 to The MaTurE cOding SEquencE of A geNe encodIng a secREtoRy PROTeIn.
The pro sequence enCoDES a Pro peptiDe that SErVeS As a cis-acTiNg cHapeRONe as tHe prOTeIn moves tHrough THe SeCretory PaThway.
A Xe2X80x9cProTeinxE2X80x9d iS a macrOmoleculE compRIsing one or more POlypeptide chaINs. a PRoTEin mAy aLso comprise Non-pEPtidIc coMPoNenTS, such As carBoHYdRAte groups. CarbohydraTeS and otHer noN-peptidic subsTituents maY BE Added to a ProtEIn BY the cell in whicH the pRotein is prODuced, and Will vAry with tHe type of cELL. ProteinS arE coMmoNly DEFiNed in terms of tHEIr amInO acid baCkbOne struCtuRes; SubStiTuEnts such aS carbohyDrAtE gRoUps Are geNErally noT sPecIfIed, But maY Be presEnt noNethElEsS.
the Term xe2x80X9CsECRetoRy SiGnal SeqUeNcexe2X80x9d dENotEs a DNA sEquence thAt eNCodeS a PoLypeptiDe (a xe2x80x9csecreToRy peptidexE2x80X9d) tHat, as a COMponent oF a larger polypeptide, direCTs the laRgeR polyPeptIde througH a sEcretoRy pAThway oF a cell In whiCh It iS SYntheSIZeD. ThE lArger polypEPTidE is coMmOnly Cleaved To remove the secretoRY pePtide during trAnsIt thROUgH thE SeCRetOrY PatHWAy. A secretory pepTide and a prO pEPtIde may be coLLecTiVely refErred to As a prE-Pro peptIde.
All refErences ciTeD herEIN arE incorporaTed by refErence in tHEir enTIrEty.
ThE prEsent iNvEntIon prOvides ISolAted DnA moleCuLes comPrisiNg A Pichia mEthanolica glyceraldehydE-3-phospHate dEhyDrogenase (gAPdh) geNe promoteR. The Invention aLso ProVides IsoLAted DnA moLEcUleS ComprIsinG A P. methanoLIc
d.
The term xe2x80x9cpromo terxe2x80x 9d is  us edhe rein  for  its art-recog n ized  meaning to denote a p ortio no f ag en e con taining DN A seq ue nc esth a tprovi defor the  binding o f R NA  polymerasea nd  initiatio n o f transcript ion . Prom ot ers equen ces  arecommon l y, but  not alwa ys ,  found in the5 x e2 x80x 2 non-coding regi o ns of genes. Sequ enceswi t hi n pro mot ers that f un ction in thei ni t i a tio n  of transcrip tion are of t encharac te riz e d by c onsen su s  nu cleotide se quen ces. Thes e prom o ter ele m ents in cludeRNA  po lyme r as ebin di n g s i te s,T ATA  sequenc es ,and t rans c r i p tion  fa ctor  bind ing sites. Se e,in g e ner al, W atson  etal ., ed s., Mo lecul ar  Biology of the  Gen e, 4th ed .,Th e B en jamin / Cummin gsPub lishing  Compan y , I nc . , Me nlo Park, Calif.,19 8 7 .A xe2x80 x9cpro se qu e ncexe2x8 0x 9dis a D NA se quen c ethat com monlyo cc ur s immed ia tely 5 xe 2x8 0x2  to t h e ma ture c oding se quenc e  of a gene enc o ding a secret o ry p ro t ein. 
Th e pro seque ncee ncod es a pr o p e ptide  that  s e rv e s as a cis-acting c ha perone  as t he protein mo ves throug h t he secre tory pa t hway.
A xe2x80 x9cpr oteinxe2x8 0 x9d is a  macr omolecul e compris i n g one or  mo repol ype p t id e chains. A p r o tein  m ay also  co mprisenon -pe pti dic  c omponents , such a sca rb oh ydr ate g r oups. Ca rb ohy dr ate s and othernon-p epti di cs ubs tituent s  m a y  bead de d to  apr otein  byt hecell in  which th e p r otei nis  produc ed, and willva ry with th etyp e of c e l l. Prote ins are commonly define d  in ter msof th eiramino aci d b ackbon e s t ructur es; su bstit ue nts s uch a s ca rbo hy drate grou p s  ar e gen er ally  not sp ecified, but may b e  pr esent nonethe les s.
T h e  t erm xe 2 x80 x9 c sec r e tory signal seq uencexe2x8 0x 9 ddenotes aD NAse quencethat en codes a polyp eptide (a  xe2x80x9 cs ecre t o rypeptidexe2 x80x9d)that, asa  comp o ne nt of  alarger  p oly pepti de, di r ect s the  large rpolype ptide  t hrough a  secretory pathway of a  cellin wh ich  it is sy nth e siz ed. The l arge r polypept ide  is  comm onl y  clea vedt orem o ve th e se c retory pe p ti ded u ri ng transitt h r oug h the  se c retory  pat hway. A secretory peptide and aprop e pti dem ay b ecollectively r efe rr e d  to as a  p re-pro pept ide.
All  r e feren ces ci ted he rein ar e in c orpora tedbyreference  in  t h eir ent ir et y .
Thepres en t inve ntionp rovi d e s isolated DNA m olecu l e s com p ris ing a  P ichia m e than olica glyc eraldehyde- 3-phos phat e deh ydrogen as e (GAP DH)  g ene promot e r. The in venti on also  p rovi des  isola tedD N A mol ecul es  co mprisinga P. me th a nol ic
d.
The term_xe2x80x9cpromoterxe2x80x9d is_used herein for its_art-recognized meaning_to_denote a_portion_of a gene_containing DNA sequences_that provide for the_binding of RNA_polymerase_and initiation of transcription. Promoter sequences are commonly, but not always, found in the_5xe2x80x2_non-coding regions_of_genes._Sequences within promoters that function_in the initiation of transcription_are often_characterized by consensus nucleotide sequences. These promoter elements_include_RNA polymerase binding_sites, TATA sequences, and transcription factor binding sites. See,_in general, Watson et al., eds.,_Molecular Biology of_the_Gene,_4th ed., The Benjamin/Cummings_Publishing Company, Inc., Menlo Park, Calif.,_1987.
A xe2x80x9cpro sequencexe2x80x9d is a DNA_sequence that commonly occurs immediately 5xe2x80x2 to_the mature coding sequence of a_gene encoding a secretory protein.
The_pro sequence_encodes a pro peptide that_serves as a_cis-acting chaperone_as the protein_moves through the secretory pathway.
A xe2x80x9cproteinxe2x80x9d_is a macromolecule_comprising one or more polypeptide chains._A_protein may also_comprise_non-peptidic_components, such_as carbohydrate groups._Carbohydrates_and other_non-peptidic_substituents may be added to a_protein_by the cell in which the protein_is produced, and will_vary_with the type of_cell. Proteins are commonly defined_in terms of their amino acid_backbone structures;_substituents such_as carbohydrate groups are generally not specified, but may be present_nonetheless.
The term xe2x80x9csecretory signal sequencexe2x80x9d denotes_a DNA sequence that_encodes a_polypeptide_(a xe2x80x9csecretory peptidexe2x80x9d)_that,_as a_component of a larger polypeptide, directs the_larger polypeptide_through a secretory pathway of a_cell in which it_is_synthesized. The larger polypeptide is commonly_cleaved to remove the secretory peptide_during transit through the secretory_pathway._A_secretory peptide and a pro_peptide may be collectively referred to_as a pre-pro_peptide.
All references cited herein are incorporated by_reference_in their entirety.
The present invention provides_isolated_DNA molecules comprising a Pichia methanolica_glyceraldehyde-3-phosphate_dehydrogenase_(GAPDH) gene promoter. The invention_also provides isolated DNA molecules comprising_a P. methanolic