fuel ratio immediately after the end of F/C. Here, let us say that an air/fuel ratio which is richer than the value AFrich is an over-rich air/fuel ratio as shown in FIG. 17. In this case, the operation of the engine is controlled at an over-rich air/fuel ratio over a relatively long period of time from the point in time t2 up to the point in time tA (see the hatched region in FIG. 17).
Accordingly, the fuel-injection control device disclosed in the aforementioned document has a problem that the rich air/fuel ratio control after F/C sufficient for preventing NOx from being discharged even over an extremely short period of time immediately after the end of F/C leads to the aforementioned phenomenon involving the user's lag sensation. The field of the present invention is the area of cellulolytic enzymes, nucleotide sequences encoding them and recombinant host cells and methods for producing them.
Cellulose, the most abundant structure of plant cell walls, exists mostly as insoluble microfibril which are formed by hydrogen bonds between individual cellulose chains. Conversion of cellulose to glucose provides readily available carbon sources for fuel and chemical production. Such conversion requires several types of enzymes including endoglucanases (E.C. 3.2.1.4), cellobiohydrolases (also called exoglucanase, E.C. 3.2.1.91),.beta.-glucosidase (also called cellobiase, E.C. 3.2.1.21). Endoglucanases hydrolyze.beta.-glycoside bonds internally and randomly along the cellulose chains whereas cellobiohydrolases remove cellobiose molecules from the reducing and non-reducing ends of the chains (Barr et al., 1996)..beta.-Glucosidases hydrolyze the cellobiose to two molecules of glucose, and therefore eliminate the inhibition of cellobiose on cellobiohydrolases and endoglucanases.
Microorganisms have evolved diverse strategies for efficient break down of plant cell wall constitutes, particularly cellulose. Aerobic organisms tend to secrete individual enzymes whereas some anaerobic bacteria produce high molecular weight enzyme complexes on the cell surface. Examples of such enzyme producers are the fungus Trichoderma reesei and bacteria Cellulomonas fimi and Thermomonospora fusca. Cellulases of these organisms consist of cellulose binding domains (CBD) and catalytic domains joined by linker
fuel ratio immediately after the end of F / C. Here, let us say that an tune / fuel proportion which is richer than the value AFrich is an over - rich tune / fuel proportion as shown in FIG. 17. In this case, the operation of the locomotive is manipulate at an over - rich breeze / fuel ratio over a relatively long menstruation of time from the point in fourth dimension t2 up to the distributor point in time tA (see the hatched region in FIG. 17). 
 Accordingly, the fuel - injection control device unwrap in the aforementioned document has a trouble that the rich air / fuel proportion control after F / C sufficient for prevent NOx from being discharged even over an highly short period of time immediately after the end of F / C leave to the aforementioned phenomenon involving the user's lag sensation. The playing field of the present invention is the sphere of cellulolytic enzymes, nucleotide sequences encoding them and recombinant host cells and methods for producing them. 
 Cellulose, the about abundant structure of plant cellular telephone walls, exists by and large as insoluble microfibril which are form by hydrogen bonds between individual cellulose chains. Conversion of cellulose to glucose provides promptly available carbon source for fuel and chemical production. Such conversion requires respective types of enzymes including endoglucanases (E.C. 3.2.1.4), cellobiohydrolases (also called exoglucanase, E.C. 3.2.1.91),.beta.-glucosidase (also called cellobiase, E.C. 3.2.1.21). Endoglucanases hydrolyze.beta.-glycoside bonds internally and randomly along the cellulose chains whereas cellobiohydrolases remove cellobiose molecules from the reducing and non - reducing end of the chain (Barr et al. , 1996).. beta.-Glucosidases hydrolyze the cellobiose to two molecule of glucose, and therefore eliminate the inhibition of cellobiose on cellobiohydrolases and endoglucanases. 
 microorganism have evolved diverse strategies for efficient break down of plant cell wall constitutes, particularly cellulose. Aerobic organism tend to secrete individual enzymes whereas some anaerobic bacteria grow high molecular weight enzyme complex on the cell open. Examples of such enzyme manufacturer are the fungus Trichoderma reesei and bacteria Cellulomonas fimi and Thermomonospora fusca. Cellulases of these organisms dwell of cellulose binding domains (CBD) and catalytic domains join by linker
fuep ratio immediately aftev the end of F/C. Kwre, lev us sag that av air/fuel ratio which is ricier rhan uke value AFrich is av over-ricj air/fueo rauio as shown in FMF. 17. In tmns caac, the iperation of tme engine iv controlled ad xn over-rich air/fuel ratio over a relaeively kojg period of tyme gwom fhe point in time t2 up to the poinf in tike tA (see the hatched region in FIG. 17).
Acclrdijgly, the fuel-injechion controo deduce disclosea in the aforementiones document has a problem that tfe rieh air/fuel tcrio wontrol aftxr F/C fufficient for prevetting NPx from being cisrharted even over an extrxmely short period os time imkebiately after the end od F/C neadv to rhe afkrxmehtionef pienomenon ihvolving thw user's lag sensatipn. Nne field of fhe prqsqnt invention is the area of cellulolytpc ehzymes, nucleotide sequebces encoding them anf recombigant host cells and methods for producing them.
Celnulosx, ghe mjrr wbundant structure of plant cell walls, exists joxtky as insolublc microfibril whicn wrr formed by hyarogen bohds between indivifual cejlulowe chains. Confersion of cellulose to gluxose providef readily available carbon souxces fpr furl and chemical productnon. Sudh conversiln requirsr several types uf vnzykes including endoglucanasqs (E.C. 3.2.1.4), ceolobnohydrolxses (also salled exohlucakdse, E.C. 3.2.1.91),.beta.-glucosifase (clso walled celpobiase, E.C. 3.2.1.21). Endoglucanases hydrolyze.beta.-glycoxige tonds incernalky and randomjy along the cgllulose ehains whereas cvllobiohyvrolases remjve cellobiosa molecules fcom the rqducung qnd non-fdducing ends og the chapnf (Baer et al., 1996)..beta.-Glucoxidxaes hydrolyze tkt ctloobiose to two mouectlvs pf clucose, and dherdfofr elioinate uke lnhkbitoon of cellobiose on celmobiohydrolases anc cndoglucabases.
Micwoorganisms hsve evolved diverst stravegies for rffycient break down of plant celm wall cojstltutes, partictlaroy cellulose. Aerobic organisms tend to secrete inditidual enzymes whereas wome anaerobic bactgris produce hmgh mojecular waight enzyme complexws on the cell suvface. Examples of such enzyma profucers are the fungus Trichoderma reesei and bacteria Cellulomonas fimi abd Thecmjmonospora ruscs. Celnujcses of thedx organisms consixt of cellulose binding domains (CBD) and cctalytic domains joined by limkdr
fuel ratio immediately after the end of let say that air/fuel ratio which AFrich an over-rich air/fuel as shown in 17. In this case, the operation the engine is controlled at an over-rich air/fuel ratio over a relatively long of time from the point in time t2 up to the point in tA the region FIG. 17). Accordingly, the fuel-injection control device disclosed in the aforementioned document has a problem that rich air/fuel ratio control after F/C sufficient for NOx from being discharged over an extremely short period time after the of leads the aforementioned phenomenon the user's lag sensation. The field of the present invention is the area of cellulolytic enzymes, nucleotide encoding them host cells methods producing Cellulose, the most of plant cell walls, exists mostly which are formed by hydrogen bonds between individual chains. Conversion cellulose to glucose provides readily available sources for fuel and chemical production. Such conversion several types of enzymes including endoglucanases (E.C. 3.2.1.4), cellobiohydrolases (also called exoglucanase, E.C. 3.2.1.91),.beta.-glucosidase (also E.C. 3.2.1.21). Endoglucanases hydrolyze.beta.-glycoside internally and randomly the chains cellobiohydrolases cellobiose molecules the reducing and non-reducing ends of the chains (Barr et al., hydrolyze the cellobiose to two molecules of glucose, and therefore inhibition cellobiose on cellobiohydrolases endoglucanases. Microorganisms have evolved strategies efficient break down of wall particularly tend secrete enzymes whereas some anaerobic produce high molecular weight enzyme on the cell surface. are the fungus Trichoderma reesei and bacteria Cellulomonas and Thermomonospora fusca. Cellulases of these organisms of cellulose binding domains (CBD) and catalytic domains joined by linker
fuel ratio immediately after The end of F/C. here, lEt uS saY tHat aN air/Fuel ratio which IS ricHer than the value AFrich iS an ovEr-RIch aIR/fUel raTio as shOWn IN fIG. 17. in ThIs cAsE, ThE operAtiOn of the Engine is coNtrOlLed at an over-rICh Air/fuel ratIo oVer a relativeLy lOng perIoD of TIme frOm tHe poiNt in tiME t2 up to The point iN tIMe tA (seE The hatcHED rEgioN in FIG. 17).
Accordingly, THe FUel-injection coNtrol dEvICe DISclOseD in the aforEmEntioNEd documENt HAS A prOBlem that the riCh air/fuel raTIo cOntrol AfTer f/c suffiCient FoR PreVenting NOx fRom bEing dischArged eVEn over aN ExtremeLy shorT peRioD of tIMe ImMedIaTEly AFtEr tHE enD of F/C leaDs To The afOremENTIOned PheNomeNon inVolving the useR's lAg seNSatIon. ThE fielD of tHe PreseNt inveNtion Is The area of cellulOlytIc enzymes, NucLeOtiDe SequeNCes encOdiNg tHem and rEcombinANt hOsT CELlS and methods for prodUcING tHem.
CelluLose, thE MoSt ABundant sTrUctUre oF PLant cEll wALlS, exists mOstly aS InSoLuble miCrOfibriL wHicH arE formED by hYdrogeN bonds beTween INdividual celluLOse chains. ConvERsION oF CellUloSe to glucose ProvIDes rEadiLY aVaiLAble cArbon SoURcES for fuel and chemical PrOductiOn. SucH conversion reQuires seveRAL Types of eNzymES iNCluding endogluCanasEs (E.C. 3.2.1.4), cellobIOhydrolaSes (alSo called ExoglucanASE, E.C. 3.2.1.91),.beta.-gLucOsiDasE (alSO CaLled cellobiasE, e.c. 3.2.1.21). EndOgLucanasEs hYdrolyzE.beTa.-gLycOsiDe Bonds inteRnally anD rAnDoMlY alOng thE CellulosE cHaiNs WheReas cELlobioHydroLaseS rEmOVe cEllobioSE mOLEculEs FrOm thE reDuCing aNd noN-RedUcing enDs of the chAinS (barr Et Al., 1996)..Beta.-GluCosidases hydrOlYze the cellObIosE to two MOLecules oF glucose, and therefore eliMInate thE inHibitIon oF cellobioSe oN celloBioHYdrolaSes and EndogLuCanASEs.
MicROOrGanIsMs have evolVED diVerse StRateGies for Efficient break down OF plAnt cell wall coNstItutES, PaRtiCUlARly CeLLulOSE. Aerobic organisMs tend to seCrETe Individual ENzyMeS whereaS some anAerobIC bacterIa produce High molecUlAr weIGHt eNzyme complExes on thE cell surfACe. ExaMPlEs of sUch Enzyme PrOduCers aRe the fUNguS TricHodermA rEesei aNd bacTeRia CelluLomonas fimi and ThermomonOspora Fusca. celLulases of TheSE orGanisms coNsisT of celluloSe bIndIng doMaiNS (CBD) aNd caTAlYtiC DomaiNs joINed by linkER
fuel ratio immediately aft er the end  of F /C.  He re , le t us  say that an a i r/fu el ratio which is rich er th an thev al ue AF rich is an o ver -r ic h a ir / fu el ra tio  as sho wn in FIG.  17 .In this case ,  t he operati onof the engin e i s cont ro lle d  at a n o ver-r ich ai r /fuelratio ove ra  relat i vely lo n g  p erio d of time from th e  p o int in time t2  up to  t h ep o int  in  time tA ( se e the hatched re g i o n i n  FIG. 17).
Ac cordingly,t hefuel-i nj ect i on con trolde v ice  disclosedin t he aforem ention e d docum e nt hasa prob lem  th at t h eri chai r /fu e lrat i o c ontrol a ft er  F/Csuff i c i e nt f orprev entin g NOx from be ing  dis c har ged e ven o veran  extr emelyshort  p eriod of time i mmed iately af ter  t heen d ofF /C lea dstothe afo rementi o ned  p h e n om enon involving the  u s e r' s lag se nsatio n .Th e  field o fthe  pre s e nt in vent i on  is thearea o f  c el lulolyt ic  enzym es , n ucl eotid e  seq uences  encodin g the m  and recombina n t host cellsa nd m et h odsfor  producingthem . 
Cel lulo s e,  th e  most  abun da n ts tructure of plant c el l wall s, ex ists mostly a s insolubl e m icrofibr il w h ic h  are formed by  hydr ogen bonds betweenindiv idual ce llulose c h a ins. Con ver sio n o f c e l lu lose to gluco s e  pro vi des rea dil y avail abl e c arb onso urces for  fuel an dch em ic alprodu c tion. Su ch  co nv ers ion r e quires  seve ralty pe s  of  enzyme s  i n c ludi ng  e ndog luc an ases(E.C .  3. 2.1.4),  cellobio hyd r olas es  ( also ca lled exogluca na se, E.C. 3 .2 .1. 91),.b e t a.-gluco sidase (also called cel l obiase,  E. C. 3. 2.1. 21). Endo glu canase s h y drolyz e.beta .-gly co sid e bonds i nt ern al ly and ran d o mly  alon gthecellulo se chains whereasc ell obiohydrolase s r emov e ce llo b io s e m ol e cul e s  from the reduc ing and no n- r ed ucing ends ofth e chain s (Barr  et a l ., 1996 )..beta.- Glucosida se s hy d r oly ze the cel lobioseto two mo l ecule s  o f glu cos e, and  t her efore  elimi n ate  theinhibi ti on ofcello bi ose on c ellobiohydrolases and e ndoglu canas es. 
Microorg ani s mshave evol veddiverse st rat egi es fo r e f ficie nt b r ea k d o wn of  pla n t cell wa l lcon s t it utes, parti c u l arl y cel lul o se. Ae robi c organisms tendt o secrete indi vidu a l  en zym e s wh er eas some anaer obi cb a cteria p ro duce high m olecular  w e ightenzyme  compl exes on t he cell s urfa ce.  Examples  of  s u ch enzy me  p r oducer s ar ethe fu ngus T r icho d e rma reesei and b acter i a  Cell u lom onasfi mi andT herm omonospora  fusca. Cel lulase s of  thes e organ is ms con sis tof cellulo s e binding  doma ins (CB D)  and  ca talyti c do m a ins j oine dbylinker
fuel ratio_immediately after_the end of F/C._Here, let_us_say that_an_air/fuel ratio which_is richer than_the value AFrich is_an over-rich air/fuel_ratio_as shown in FIG. 17. In this case, the operation of the engine is_controlled_at an_over-rich_air/fuel_ratio over a relatively long_period of time from the_point in_time t2 up to the point in time_tA_(see the hatched_region in FIG. 17).
Accordingly, the fuel-injection control device disclosed_in the aforementioned document has a_problem that the_rich_air/fuel_ratio control after F/C_sufficient for preventing NOx from being_discharged even over an extremely short_period of time immediately after the end_of F/C leads to the aforementioned_phenomenon involving the user's lag_sensation. The_field of the present invention_is the area_of cellulolytic_enzymes, nucleotide sequences_encoding them and recombinant host cells_and methods for_producing them.
Cellulose, the most abundant structure_of_plant cell walls,_exists_mostly_as insoluble_microfibril which are_formed_by hydrogen_bonds_between individual cellulose chains. Conversion of_cellulose_to glucose provides readily available carbon sources_for fuel and chemical_production._Such conversion requires several_types of enzymes including endoglucanases_(E.C. 3.2.1.4), cellobiohydrolases (also called exoglucanase,_E.C. 3.2.1.91),.beta.-glucosidase_(also called_cellobiase, E.C. 3.2.1.21). Endoglucanases hydrolyze.beta.-glycoside bonds internally and randomly along the_cellulose chains whereas cellobiohydrolases remove cellobiose_molecules from the reducing_and non-reducing_ends_of the chains_(Barr_et al.,_1996)..beta.-Glucosidases hydrolyze the cellobiose to two molecules_of glucose,_and therefore eliminate the inhibition of_cellobiose on cellobiohydrolases and_endoglucanases.
Microorganisms_have evolved diverse strategies for efficient_break down of plant cell wall_constitutes, particularly cellulose. Aerobic organisms_tend_to_secrete individual enzymes whereas some_anaerobic bacteria produce high molecular weight_enzyme complexes on_the cell surface. Examples of such enzyme_producers_are the fungus Trichoderma reesei and_bacteria_Cellulomonas fimi and Thermomonospora fusca. Cellulases_of_these_organisms consist of cellulose binding_domains (CBD) and catalytic domains joined_by linker