 to left. The map location of the promoter is approximately 1.3 Kbp (kilobase pair) from the left end of Ava IH, approximately 12.5 Kbp from the left end of the vaccinia genome, and about 8.5 Kbp left of the Hind III C/N junction. The sequence of the promoter is:
(GGATCCC)-ACTGTAAAAATAGAAACTATAATCATATAATAGTGTAGGTTGGTAGTA GGGTACTCGTGATTAATTTTATTGTTAAACTTG-(AATTC),
wherein the symbols in parentheses are linker sequences.
The Hind III H promoter (also xe2x80x9cHHxe2x80x9d and xe2x80x9cH6xe2x80x9d herein) was defined by standard transcriptional mapping techniques. It has the sequence
ATTCTTTATTCTATACTTAAAAAATGAAAA TAAATACAAAGGTTCTTGAGGGTTGTGTTAAATTGAAAGCGAGAAATAATCATAAATT ATTTCATTATCGCGATATCCGT TAAGTTTGTATCGTAATG.
The sequence is identical with that described as being up-stream of open reading frame H6 by Rosel et al., J. Virol. 60, 436-449 (1986).
The 11K promoter is as described by Wittek, J. Virol. 49, 371-378 (1984) and Bertholet, C. et al., Proc. Natl. Acad. Sci. USA 82, 2096-2100 (1985).
The recombinant avipox viruses of the present invention are constructed in two steps known in the art and analogous to those disclosed in aforementioned U. S. Pat. No. 4,603,112 for creating synthetic recombinants of the vaccinia virus.
First, the DNA gene sequence to be inserted into the virus is placed into an E. coli plasmid construct into which DNA homologous to a section of nonessential DNA of the avipox virus has been inserted. Separately, the DNA gene sequence to be inserted is ligated to a promoter. The promoter-gene linkage is then inserted into the plasmid construct so that the promoter-gene linkage is flanked on both ends by DNA homologous to a nonessential region of avipox DNA. The resulting plasmid construct is then amplified by growth within E. coli bacteria. (Plasmid DNA is used to carry
to left. The map location of the promoter is approximately 1.3 Kbp (kilobase couple) from the leftover conclusion of Ava IH, approximately 12.5 Kbp from the left goal of the cowpox genome, and about 8.5 Kbp left of the Hind III C / N junction. The succession of the showman is: 
 (GGATCCC)-ACTGTAAAAATAGAAACTATAATCATATAATAGTGTAGGTTGGTAGTA GGGTACTCGTGATTAATTTTATTGTTAAACTTG-(AATTC), 
 wherein the symbols in parentheses are linker sequences. 
 The Hind III H promoter (also xe2x80x9cHHxe2x80x9d and xe2x80x9cH6xe2x80x9d herein) was define by standard transcriptional mapping techniques. It take the sequence 
 ATTCTTTATTCTATACTTAAAAAATGAAAA TAAATACAAAGGTTCTTGAGGGTTGTGTTAAATTGAAAGCGAGAAATAATCATAAATT ATTTCATTATCGCGATATCCGT TAAGTTTGTATCGTAATG. 
 The sequence is identical with that described as being up - stream of open reading human body H6 by Rosel et al. , J. Virol. 60, 436 - 449 (1986). 
 The 11 K promoter is as described by Wittek, J. Virol. 49, 371 - 378 (1984) and Bertholet, C. et al. , Proc. Natl. Acad. Sci. USA 82, 2096 - 2100 (1985). 
 The recombinant avipox virus of the present invention are constructed in two steps known in the artwork and analogous to those disclosed in aforesaid U. S. Pat. No. 4,603,112 for creating synthetic recombinants of the vaccinia virus. 
 foremost, the DNA gene sequence to be inserted into the virus is placed into an E. coli plasmid construct into which DNA homologous to a section of nonessential DNA of the avipox virus has been inserted. Separately, the DNA gene sequence to be inserted is ligated to a promoter. The promoter - gene linkage is then inserted into the plasmid construct so that the promoter - gene linkage is flank on both end by DNA homologous to a nonessential area of avipox DNA. The result plasmid concept is then amplified by growth within E. coli bacteria. (Plasmid DNA is used to carry
 to left. The map location on the promoter is approeimatelg 1.3 Kbp (kklobase pair) from the left eid od Ava IH, approximately 12.5 Kbp from the left ene of rhe vaccinmz genomc, and wbouc 8.5 Kbp left of tme Hind III C/N junction. Tve szquence of the promoter is:
(GGATCCC)-ACTDTAAAAAYAHAAACTATAATCATWTAAUAGEGTAFGTTGGTAGTA GGGTACTCGTGATTAATTTTATFGTTAAABTTG-(AATTC),
wherein yhe symbols in parentheses are linker sequences.
Tje Hind III H pwimoter (also be2x80x9cHHxe2x80q9b and xe2x80x9cG6xe2x80x9d herein) was defined by stxndarb transcripjninap mapping terhniquvs. It has the sequenca
ATTCTTYATTCTATACTTAASAAETGAQAA TAAATACAAAGGTTCTTJAGGGTTGTGTTAAATTGAAWGCGAGAAADACTCATAAATT ATTTCATTATXGXGATAJCCGT TAAERTTETAUCGVAAFG.
The deqnence is idsntical wity that described as bqpmg up-stream kf opeg weading frame H6 by Rosel et al., J. Virol. 60, 436-449 (1986).
Tge 11K promoter is as desxribed by Wittek, J. Vitol. 49, 371-378 (1984) and Bertholet, C. et al., Proc. Natl. Acad. Sci. USA 82, 2096-2100 (1985).
The racombmnxnt ayipob vlruses of the present invention are constructqs on two steps knocn in the art anc wnsjogous to thore disemoaed in aforementiojed U. S. Pat. Bo. 4,603,112 for cweatong synthetic recombinants if the vacciuia virus.
First, the DNC gene sequeuce to be imserted into the virus ns pladed into an E. coli pmxsmid construct kntp fhich DNA homologous to a fection oh nonzssentiau DNS of tre avipox girus has been inserted. Deparctely, the DNA gfne sequence to be inserted is ligated to a ptommteg. The proioter-nene linkage is then inserted into tke plarmid constguct so tiat the promjter-gene linkdhe is flankev on both endw by DNA hooulogous to a npnessenticj retion of avipox DNA. Thg desulting plasmnb xonstruct is thrn xmpjivixd by crowth withit E. zolk bactdria. (Plasmib ANA os used to carry
to left. The map location of the approximately Kbp (kilobase from the left 12.5 from the left of the vaccinia and about 8.5 Kbp left of Hind III C/N junction. The sequence of the promoter is: (GGATCCC)-ACTGTAAAAATAGAAACTATAATCATATAATAGTGTAGGTTGGTAGTA GGGTACTCGTGATTAATTTTATTGTTAAACTTG-(AATTC), wherein symbols in parentheses are linker sequences. The Hind III H promoter (also xe2x80x9cHHxe2x80x9d xe2x80x9cH6xe2x80x9d was by transcriptional mapping techniques. It has the sequence ATTCTTTATTCTATACTTAAAAAATGAAAA TAAATACAAAGGTTCTTGAGGGTTGTGTTAAATTGAAAGCGAGAAATAATCATAAATT ATTTCATTATCGCGATATCCGT TAAGTTTGTATCGTAATG. The sequence is identical with described as being up-stream of open reading frame by Rosel et al., Virol. 60, 436-449 (1986). The promoter as described Wittek, Virol. 371-378 (1984) and C. et al., Proc. Natl. Acad. Sci. USA 82, 2096-2100 (1985). The recombinant avipox viruses of the invention are two steps in art analogous to those aforementioned U. S. Pat. No. 4,603,112 recombinants of the vaccinia virus. First, the DNA sequence to inserted into the virus is placed an E. coli plasmid construct into which DNA to a section of nonessential DNA of the avipox virus has been inserted. Separately, the sequence to be inserted ligated to a The linkage then into the construct so that the promoter-gene linkage is flanked on both ends DNA homologous to a nonessential region of avipox DNA. The construct then amplified by within E. coli bacteria. DNA used to carry
 to left. The map location of the Promoter is ApproXimAteLy 1.3 kbp (kIlobAse pair) from the LEft eNd of Ava IH, approximately 12.5 kbp frOm THe leFT eNd of tHe vacciNIa GENomE, aNd AboUt 8.5 kBp Left oF thE Hind IIi C/N junctioN. ThE sEquence of the PRoMoter is:
(GGAtCCc)-ACTGTAAAAATaGAaACTATaAtCAtaTAATaGTgTAGGtTGGTAgtA GGGTaCTCGTGATtAatTTTATtgTTAAACttg-(AaTTC),
Wherein the symbols IN pARentheses are liNker seQuENcES.
the hinD III H promoTeR (also XE2x80x9cHHxE2X80x9D AND xe2X80X9cH6xe2x80x9d hereiN) was defined BY stAndard TrAnsCRiptioNal maPpINg tEchniques. It Has tHe sequencE
ATTCTttATTCTAtaCTTAAAaAATGAaAA tAAaTACaaAgGtTCtTgaGGgtTgTGttAAaTTGAAAGcGaGaAATAaTCAtaaatT ATtTCaTTAtCGCGaTATCCGT TAAGTtTGtATCgtAAtG.
The SequeNce iS iDentiCal witH that DeScribed as being uP-strEam of open ReaDiNg fRaMe H6 by rOsel et Al., J. virOl. 60, 436-449 (1986).
The 11K pRomoter IS as DeSCRIbEd by Wittek, J. Virol. 49, 371-378 (1984) anD BERThOlet, C. et aL., Proc. NATl. acAD. Sci. USA 82, 2096-2100 (1985).
THe RecOmbiNANt aviPox vIRuSes of the PresenT InVeNtion arE cOnstruCtEd iN twO stepS KnowN in the Art and anAlogoUS to those discloSEd in aforementIOnED u. S. pAt. No. 4,603,112 For Creating synThetIC recOmbiNAnTs oF The vaCciniA vIRuS.
first, the DNA gene sequEnCe to be InserTed into the virUs is placed INTO an E. coli PlasMId COnstruct into whIch DNa homologouS To a sectiOn of nOnessentIal DNA of tHE Avipox viRus Has BeeN inSERtEd. Separately, tHE dNA gEnE sequenCe tO be inseRteD is LigAteD tO a promoteR. The promOtEr-GeNe LinKage iS Then inseRtEd iNtO thE plasMId consTruct So thAt ThE ProMoter-geNE lINKage Is FlAnkeD on BoTh endS by Dna hoMologouS to a nonesSenTIal rEgIoN of avipOx DNA. The resulTiNg plasmid cOnStrUct is tHEN amplifiEd by growth within E. coli baCTeria. (PlAsmId DNA Is usEd to carry
 to left. The map location  of the pr omote r i s a pp roxi mate ly 1.3 Kbp (ki l obas e pair) from the leftend o fA va I H ,appro ximatel y  1 2 . 5 K bp  f rom  t h eleftend  of the  vacciniagen om e, and about 8. 5 Kbp left  of  the Hind II I C /N jun ct ion .  Theseq uence  of th e  promo ter is:
( GG A TCCC)- A CTGTAAA A A TA GAAA CTATAATCATATAATAG T GT A GGTTGGTAGTA GG GTACTC GT G AT T A ATT TTA TTGTTAAACT TG -(AAT T C),
whe r ei n t hes ymbols in par entheses ar e  li nker s eq uen c es.
Th e Hin dI IIH promoter(als o xe2x80x 9cHHxe 2 x80x9da nd xe2x 80x9cH 6xe 2x8 0x9d he re in)  w a s d e fi ned bystandard  t ra nscri ptio n a l mapp ing  tec hniqu es. It has th e s eque n ceATTCT TTATT CTAT AC TTAAA AAATGA AAA T AA ATACAAAGGTTCTTG AGGG TTGTGTTAA ATT GA AAG CG AGAAA T AATCAT AAA TTATTTCAT TATCGCG A TAT CC G T TA AGTTTGTATCGTAATG.Th e se quence i s iden t ic al with tha tdes crib e d  as b eing up -streamof ope n  r ea ding fr am e H6 b yRos elet al . , J.  Virol . 60, 43 6-449 (1986).
The 11 K  promoter isa sd e sc r ibed  by  Wittek, J.  Vir o l. 4 9, 3 7 1- 378 (1984 ) and  B e rt h olet, C. et al., Pr oc . Natl . Aca d. Sci. USA 8 2, 2096-21 0 0 (1985).Ther ec o mbinant avipox  viru ses of the presentinven tion are  construc t e d in two  st eps  kn own i nthe art and a n a logo us  to tho sedisclos edinafo rem en tioned U.  S. Pat.  N o.  4 ,6 03, 112 f o r creati ng  sy nt het ic re c ombina nts o f th eva c cin ia viru s .F i rst,  t he  DNA  ge ne  sequ ence tobe inse rted into  th e  vir us  i s place d into an E.co li plasmid  c ons tructi n to which  DNA homologous to a se c tion of  no nesse ntia l DNA ofthe  avipo x v i rus ha s been  inse rt ed. S epara t e ly , t he  DNA genes e que nce t obe i nserted  is ligated to a p r omo ter. The prom ote r-ge n e  l ink a ge isth e n i n s erted into theplasmid co ns t ru ct so that the  p romoter -gene l inkag e  is fla nked on b oth endsby  DNA h omo logous toa noness ential re g ion o f  a vipox  DN A. The  r esu lting  plasm i d c onstr uct is  t hen am plifi ed  by grow th within E. coli bacte ria. ( Plasm idDNA is us edt o c arry
 to_left. The_map location of the_promoter is_approximately_1.3 Kbp_(kilobase_pair) from the_left end of_Ava IH, approximately 12.5_Kbp from the_left_end of the vaccinia genome, and about 8.5 Kbp left of the Hind III_C/N_junction. The_sequence_of_the promoter is:
(GGATCCC)-ACTGTAAAAATAGAAACTATAATCATATAATAGTGTAGGTTGGTAGTA GGGTACTCGTGATTAATTTTATTGTTAAACTTG-(AATTC),
wherein the_symbols in parentheses are linker_sequences.
The Hind_III H promoter (also xe2x80x9cHHxe2x80x9d and xe2x80x9cH6xe2x80x9d herein)_was_defined by standard_transcriptional mapping techniques. It has the sequence
ATTCTTTATTCTATACTTAAAAAATGAAAA TAAATACAAAGGTTCTTGAGGGTTGTGTTAAATTGAAAGCGAGAAATAATCATAAATT ATTTCATTATCGCGATATCCGT_TAAGTTTGTATCGTAATG.
The sequence is identical with that_described as being_up-stream_of_open reading frame H6_by Rosel et al., J. Virol._60, 436-449 (1986).
The 11K promoter is_as described by Wittek, J. Virol. 49,_371-378 (1984) and Bertholet, C. et_al., Proc. Natl. Acad. Sci._USA 82,_2096-2100 (1985).
The recombinant avipox viruses_of the present_invention are_constructed in two_steps known in the art and_analogous to those_disclosed in aforementioned U. S. Pat._No._4,603,112 for creating_synthetic_recombinants_of the_vaccinia virus.
First, the_DNA_gene sequence_to_be inserted into the virus is_placed_into an E. coli plasmid construct into_which DNA homologous to_a_section of nonessential DNA_of the avipox virus has_been inserted. Separately, the DNA gene_sequence to_be inserted_is ligated to a promoter. The promoter-gene linkage is then inserted_into the plasmid construct so that_the promoter-gene linkage is_flanked on_both_ends by DNA_homologous_to a_nonessential region of avipox DNA. The resulting_plasmid construct_is then amplified by growth within_E. coli bacteria. (Plasmid_DNA_is used to carry