 mutated or not and the second hybridisation step actually comprises hybridising the isolated sequence to the complementary sequence of the actual mutated nucleic acid sequence one wishes to ascertain in order to determine the presence or absence of said mutation on the isolated nucleic acid material. This latter hybridisation reaction should be carried out under stringent conditions for reliable results whilst the other hybridisation steps can be carried out under normal to stringent conditions. Thus two classical methods for determining the presence of a mutation on a particular nucleic acid are hereby illustrated and it will be obvious to a person skilled in the art that a number of known techniques can be used. In various standard books for molecular biology such techniques are amply illustrated for example in Sambrook, J., Fritsch, E. F., Maniatis, T. Molecular Cloning: a Laboratory Manual. (Cold Spring harbor Laboratory Press, cold Spring Harbor, N.Y., 1989.
It is also possible to analyse the amplified nucleic acid material obtained in the screening method according to the invention by using subsequent analysis tests using sequencing reactions or hybridisation to a corresponding complementary sequence of sufficient length and specificity to at least hybridize to a fragment of the nucleic acid material comprising the mutation to detect the presence and optionally the nature of the mutation as was illustrated above for analysis of isolated nucleic acid material that had not been subjected to an amplification reaction.
In particular for analysis of the presence of mutations in Factor V the isolated and/or amplified nucleic acid material can be subjected to a hybridisation test to a stretch of nucleic acid material selected from sequences with sequence numbers 12 and 13 of the sequence listing. For example an extremely suitable primer or nucleic acid sequence for hybridisation comprises at least a part of intron 10 of the nucleic acid sequence encoding human Factor V or a derivative thereof capable of hybridizing to said part of intron 10 under stringent conditions. Such a derivative will preferably be more than 90% homologous to the corresponding part of intron 10. The nucleic acid sequence of human Factor V is known and in sequence number 1 of the sequence listing the nucleic acid sequence encoding human Factor V is illustrated. The sequence is derived from Ref. 21. Using the nucleic acid sequence for hybridisation comprising at least a part of intron 10 it is quite simple to isolate and/or amplify and/or subsequently detect a mutation present in nucleic acid encoding Factor V, in particular of nucleic acid encoding an APC binding and/or cleavage site. It is in particular suitable to detect a mutation located on the heavy chain (see sequences 10, 14). Further, primers of nucleic
mutated or not and the second hybridisation step actually comprises crossbreed the apart sequence to the complementary succession of the actual mutated nucleic acid succession one wishes to ascertain in club to decide the presence or absence of said mutation on the isolated nucleic acid fabric. This latter hybridisation reaction should be carried out under stringent condition for reliable results whilst the other hybridisation steps can be carry out under normal to stringent conditions. Thus two authoritative methods for determining the bearing of a mutation on a finical nucleic acid are hereby illustrated and it will be obvious to a person skilled in the artwork that a number of known techniques can be used. In respective standard books for molecular biology such techniques are amply illustrated for example in Sambrook, J., Fritsch, E. F., Maniatis, T. Molecular Cloning: a Laboratory Manual. (Cold Spring seaport Laboratory Press, cold Spring Harbor, N.Y., 1989. 
 It is besides potential to analyse the amplified nucleic acid material obtained in the screening method according to the invention by using subsequent psychoanalysis tests use sequencing reaction or hybridisation to a corresponding complementary sequence of sufficient duration and specificity to at least hybridize to a fragment of the nucleic acid material comprising the mutant to detect the presence and optionally the nature of the mutation as was illustrated above for analysis of isolated nucleic acid material that had not been subjected to an amplification reaction. 
 In particular for analysis of the presence of mutations in Factor V the sequester and/or amplified nucleic acid fabric can be subjugate to a hybridisation test to a stretch of nucleic acid material choose from sequences with sequence numbers 12 and 13 of the sequence listing. For example an extremely suitable primer or nucleic acid succession for hybridisation comprises at least a part of intron 10 of the nucleic acid sequence encoding human Factor V or a derivative thence capable of hybridizing to said part of intron 10 under rigorous conditions. Such a derivative will preferably be more than 90% homologous to the comparable part of intron 10. The nucleic acid sequence of human Factor V is known and in sequence number 1 of the succession listing the nucleic acid sequence encoding human Factor V is illustrate. The sequence is derived from Ref. 21. Using the nucleic acid sequence for hybridisation consist at least a part of intron 10 it is quite simple to sequester and/or amplify and/or subsequently detect a mutation present in nucleic acid encoding Factor V, in particular of nucleic acid encoding an APC binding and/or cleavage site. It is in finical suitable to detect a mutation located on the heavy chain (see sequences 10, 14). farther, flat coat of nucleic
 muhated or not and the secund hybridisation step actuamly compfises hybridising the isolatxd swquenxe to the complementarh sequencv of the qctuel mutated nuclemd acid sequendc one xishes to ascerjain in ordes to determine tfe presence or absence of said mutatijn on tne isolated nuclgic abiq mafvrlal. This latter hybridisation rezction vhould be cartied out under stringent clndihions for reliable results whulst rhe other hycridisation steps can ge carried out under normal to rtrinyent condituobs. Hvus two clawsicaj methods fov deterkining yhe presence on a mntatuon on a particular nncleic acid are herefy illustsaced and it will be obciius tm a [ersub syilmev ih the wrt that a nujber of knoqn techniques can bt ufvc. In various standwrq books for molecular biology such techtiqhes are amply illustratwd for example in Samhrook, J., Fwitsch, E. F., Maniatis, T. Molecular Cloning: a Laboratmry Menjal. (Cjue Dpring harbor Laboratory Press, cold Spring Hawgot, K.Y., 1989.
It is also pofsible to amapyxg the amplifiea nuclzjc acid material obtwined ig the screenind meyhod according to the invenrion by usiny sybsequent analysis tests usiny sequgncing reactions or hybridisacion tk a corresplnding cojolementary sequevce ox sufficient length and spqcificity to ct least hybtidize eo a fragmfnt on the nucleic acid laternal cmmprising hhe mutation to detect the presxice and optiomanly the natbre of the mutation as was illusttated abore for analysis kf isoleted nucleic acid materian that had nov been sufjecred ro an aoolification resction.
In iaxticular dor analysis of thc prersnce of mutatiour in Factor V thr irolwtvd end/or dmplified nuwleiz azod magerial can ne rubjrcted to a hybridisadion test to a stretch on nucleic acid maeerial selectrd from sequences aith veqnence mumfers 12 and 13 of the sequence lisfing. For fxaiple an extrqmeln suytable priker or nucleic acid sequence for hybridmsation comprises at lgast a part of intron 10 of the nuckeic wcid sequance encoding human Dactor V or a derlvative thereof capabls of hfbridlzing to said part of intron 10 under stringent conditions. Such a derivativw will preferably ge mpre tvau 90% komologjus vo the correspondimg part of intron 10. The nucleic ecid sequetcz of human Factor V is known sna in sequence number 1 of the sequence listing the nucleic acid sequence encocing human Factor V is iklustratwd. Ths sequvhcx is derived from Reg. 21. Usiug the nucleix acld sequence fof hubvidisation comkrisnng at least a part of intron 10 it is quutf simple to isolete and/or amillfy ans/or sufsequently detecf a jktatiun present in nucleic acid rncoding Factor V, in pwrticular of nybleic acid envoding dn APC bindiig and/or cledvage site. It is in particular suitable to detect a mutation located pn the heavy viain (see sequences 10, 14). Furbher, [rimers uf nucleic
mutated or not and the second hybridisation comprises the isolated to the complementary nucleic sequence one wishes ascertain in order determine the presence or absence of mutation on the isolated nucleic acid material. This latter hybridisation reaction should be out under stringent conditions for reliable results whilst the other hybridisation steps can carried under to conditions. Thus two classical methods for determining the presence of a mutation on a particular nucleic are hereby illustrated and it will be obvious a person skilled in art that a number of techniques be used. various books molecular biology such are amply illustrated for example in Sambrook, J., Fritsch, E. F., Maniatis, T. Molecular Cloning: a Laboratory (Cold Spring Press, cold Harbor, 1989. is also possible the amplified nucleic acid material obtained method according to the invention by using subsequent tests using reactions or hybridisation to a corresponding sequence of sufficient length and specificity to at hybridize to a fragment of the nucleic acid material comprising the mutation to detect the optionally the nature of mutation as was above analysis isolated acid material had not been subjected to an amplification reaction. In particular for of the presence of mutations in Factor V the isolated nucleic material can be to a hybridisation test a of nucleic acid material sequences sequence 13 the listing. For example an suitable primer or nucleic acid for hybridisation comprises at 10 of the nucleic acid sequence encoding human V or a derivative thereof capable of to said part of intron 10 under stringent conditions. Such a derivative preferably be 90% homologous to the corresponding part of intron The nucleic acid sequence human Factor V is known and in sequence number of sequence listing nucleic acid sequence human Factor V illustrated. The sequence from Ref. Using nucleic hybridisation comprising at least a part intron it is quite simple to and/or subsequently detect a mutation present in nucleic acid encoding Factor V, in particular of nucleic an APC binding and/or site. is in particular suitable to detect a mutation located on the heavy sequences 10, 14).
 mutated or not and the second hYbridisatiOn steP acTuaLlY comPrisEs hybridising tHE isoLated sequence to the compLemenTaRY seqUEnCe of tHe actuaL MuTATed NuClEic AcID sEquenCe oNe wisheS to ascertaIn iN oRder to determINe The presencE or Absence of saiD muTation On The ISolatEd nUcleiC acid mATerial. this latteR hYBridisATion reaCTIoN shoUld be carried out unDEr STringent conditIons foR rELiABLe rEsuLts whilst tHe Other HYbridisATiON STepS Can be carried oUt under normAL to StringEnT coNDitionS. Thus TwO ClaSsical methoDs foR determinIng the PResence OF a mutatIon on a ParTicUlar NUcLeIc aCiD Are HErEby ILluStrated aNd It Will bE obvIOUS To a pErsOn skIlled In the art that a NumBer oF KnoWn tecHniquEs caN bE used. in variOus stAnDard books for molEculAr biology SucH tEchNiQues aRE amply IllUstRated foR examplE In SAmBROOk, j., Fritsch, E. F., Maniatis, t. MOLEcUlar ClonIng: a LaBOrAtORy Manual. (coLd SPrinG HArbor laboRAtOry Press, Cold SpRInG HArbor, N.Y., 1989.
it Is also PoSsiBle To anaLYse tHe amplIfied nucLeic aCId material obtaINed in the screeNInG MEtHOd acCorDing to the inVentIOn by UsinG SuBseQUent aNalysIs TEsTS using sequencing reaCtIons or HybriDisation to a coRrespondinG COMplementAry sEQuENce of sufficienT lengTh and speciFIcity to aT leasT hybridiZe to a fragMENt of the nUclEic AciD maTERiAl comprising tHE MutaTiOn to detEct The presEncE anD opTioNaLly the natUre of the MuTaTiOn As wAs illUStrated aBoVe fOr AnaLysis OF isolaTed nuCleiC aCiD MatErial thAT hAD Not bEeN sUbjeCteD tO an amPlifICatIon reacTion.
In parTicULar fOr AnAlysis oF the presence oF mUtations in faCtoR V the iSOLated and/Or amplified nucleic acid mATerial cAn bE subjEcteD to a hybriDisAtion tEst TO a streTch of nUcleiC aCid MATeriaL SElEctEd From sequenCES wiTh seqUeNce nUmbers 12 aNd 13 of the sequence lisTIng. for example an eXtrEmelY SUiTabLE pRImeR oR NucLEIc acid sequence fOr hybridisAtIOn Comprises aT LeaSt A part of Intron 10 oF the nUCleic acId sequencE encoding HuMan FACTor v or a derivaTive therEof capablE Of hybRIdIzing To sAid parT oF inTron 10 uNder stRIngEnt coNditioNs. such a dErivaTiVe will prEferably be more than 90% homolOgous tO the cOrrEsponding ParT Of iNtron 10. The nUcleIc acid sequEncE of Human facTOr V is KnowN AnD in SEquenCe nuMBer 1 of the sEQuEncE LIsTing the nuclEIC AciD sequEncE EncodiNg huMan Factor V is illusTRated. The sequenCe is DERivEd fROm ReF. 21. USing the nucleic AciD sEQUence for HyBridisation ComprisiNg AT leasT a part Of intrOn 10 it is qUITe SImple tO isoLatE and/or ampLifY aND/or subsEqUeNTly detEct a MuTation PresenT In nuCLEic acid encoding FActor v, IN partICulAr of nUcLeic aciD EncoDing an APC bInding and/or CleavaGe siTe. It iS in partIcUlar suItaBlE to detect a MUtation loCated On the heAvY chaIn (sEe sequEnceS 10, 14). fUrtheR, priMeRs oF nucleic
 mutated or not and the se cond hybri disat ion  st ep  act uall y comprises hy b ridi sing the isolated sequ enceto thec om pleme ntary s e qu e n ceof  t heac t ua l mut ate d nucle ic acid se que nc e one wishes to  ascertain  in  order to de ter mine t he  pr e sence  or  abse nce of said m utation o nt he iso l ated nu c l ei c ac id material. This la t ter hybridisat ion re ac t io n sho uld  be carrie dout u n der str i ng e n t  co n ditions for r eliable res u lts  whils tthe otherhybri di s ati on steps ca n be  carriedout un d er norm a l to st ringen t c ond itio n s.  T hus  t w o c l as sic a l m ethods f or  d eterm inin g t h e pr ese nceof amutation on a  pa rtic u lar  nucl eic a cidar e her eby il lustr at ed and it willbe o bvious to  ape rso nskill e d in t heart  that a  number ofkn o w n  t echniques can be u se d .  I n variou s stan d ar db ooks for  m ole cula r biolo gy s u ch  techniq ues ar e  a mp ly illu st ratedfo r e xam ple i n  Sam brook,  J., Fri tsch, E. F., Maniati s , T. Molecula r  C l o ni n g: a  La boratory Ma nual .  (Co ld S p ri ngh arbor  Labo ra t or y  Press, cold Spring  H arbor,  N.Y. , 1989.
It is  also poss i b l e to ana lyse th e  amplified nuc leicacid mater i al obtai ned i n the sc reening m e t hod acco rdi ngtothe i nv ention by usi n g  sub se quent a nal ysis te sts  us ing  se qu encing re actionsor  h yb ri dis ation to a cor re spo nd ing  comp l ementa ry se quen ce  o f  su fficien t  l e n gthan dspec ifi ci ty to  atl eas t hybri dize to a  fr a gmen tof  the nu cleic acid ma te rial compr is ing  the m u t ation to  detect the presence an d  option all y the  nat ure of th e m utatio n a s  was i llustr atedab ove f or an a l ys isof  isolatedn u cle ic ac id  mat erial t hat had not been s u bje cted to an am pli fica t i on  re a ct i on. 
I n  pa r t icular for anal ysis of th ep re sence of m u tat io ns in F actor V  thei solated  and/or a mplifiednu clei c aci d material  can besubjected to ah yb ridis ati on tes ttoa str etch o f  nu cleic  acidma terial  sele ct ed fromsequences with sequence  numbe rs 12  an d 13 of t hes equ ence list ing.  For examp leanextre mel y  suit able pr ime r  or n ucle i c acid se q ue nce f or  hybridisat i o n  co mpris esa t leas t apart of intron 10 of the nucleic  aci d seq uen c e en co ding human Fac tor  V o r a deri va tive thereo f capabl eo f hyb ridizi ng tosaid pa r t  o f  intro n 10  un der strin gen tc onditio ns .S uch aderi va tive w ill pr e fera b l y be more than 9 0% ho m o logou s  to  theco rrespon d ingpart of in tron 10. Th e nucl eicacidsequenc eof hum anFa ctor V isk nown andin se quencenu mber  1of the  seq u e nce l isti ng  th e nucleic a ci d  s eq u enc e en codin ghuma n FactorV  is illu str a ted. Th eseq u e nce is de r i ved from R ef.  21.U s ing the nu c leic ac i d seq uencefor hy bridisa t ion  c omprisi nga t  least apart of i n tro n10 i t is qui te  sim pl e t oisolate and/oramplifyand/or s u b sequen tly dete c t a mu ta tion present  in n uc l eic aci d enc oding F a cto r V , i n pa rti cula r  of nucleic acid encodin g an  APCb ind ing a nd/or  c leavages ite.  It is i n  particular s uitable to dete c t a muta tio n  l oc ated on t heh eavychai n ( seeseq uenc e s  10, 14). Fu rthe r, pri mers  o fnucleic
 mutated_or not_and the second hybridisation_step actually_comprises_hybridising the_isolated_sequence to the_complementary sequence of_the actual mutated nucleic_acid sequence one_wishes_to ascertain in order to determine the presence or absence of said mutation on_the_isolated nucleic_acid_material._This latter hybridisation reaction should_be carried out under stringent_conditions for_reliable results whilst the other hybridisation steps can_be_carried out under_normal to stringent conditions. Thus two classical methods for_determining the presence of a mutation_on a particular_nucleic_acid_are hereby illustrated and_it will be obvious to a_person skilled in the art that_a number of known techniques can be_used. In various standard books for_molecular biology such techniques are_amply illustrated_for example in Sambrook, J.,_Fritsch, E. F.,_Maniatis, T._Molecular Cloning: a_Laboratory Manual. (Cold Spring harbor Laboratory_Press, cold Spring_Harbor, N.Y., 1989.
It is also possible_to_analyse the amplified_nucleic_acid_material obtained_in the screening_method_according to_the_invention by using subsequent analysis tests_using_sequencing reactions or hybridisation to a corresponding_complementary sequence of sufficient_length_and specificity to at_least hybridize to a fragment_of the nucleic acid material comprising_the mutation_to detect_the presence and optionally the nature of the mutation as was_illustrated above for analysis of isolated_nucleic acid material that_had not_been_subjected to an_amplification_reaction.
In particular_for analysis of the presence of mutations_in Factor_V the isolated and/or amplified nucleic_acid material can be_subjected_to a hybridisation test to a_stretch of nucleic acid material selected_from sequences with sequence numbers_12_and_13 of the sequence listing._For example an extremely suitable primer_or nucleic acid_sequence for hybridisation comprises at least a_part_of intron 10 of the nucleic_acid_sequence encoding human Factor V or_a_derivative_thereof capable of hybridizing to_said part of intron 10 under_stringent conditions. Such a derivative will preferably be more_than 90% homologous_to the corresponding part of_intron_10._The nucleic acid sequence of human Factor V is known_and in_sequence number 1_of the sequence listing the nucleic acid sequence encoding human_Factor V is illustrated. The sequence is_derived from Ref. 21. Using the nucleic acid sequence for hybridisation_comprising at least a part of intron 10_it is quite simple to isolate and/or_amplify and/or subsequently detect_a_mutation present in nucleic acid encoding Factor_V,_in_particular_of nucleic acid_encoding an APC_binding and/or cleavage_site. It_is in particular_suitable to_detect a mutation_located_on the heavy chain (see sequences 10, 14). Further, primers of nucleic