olocarbazole aglycons to D-glucosilated derivatives. The procedure consisted of adding a particular indolocarbazole aglycon (either chemically synthesized or isolated from a producer strain) to the culture broth of a Streptomyces lividans strain harboring a plasmid containing the ngt gene, and isolating the glucosylated product from the culture (Ohuchi et al. J. Antibiot. 53: 393-403, 2000).
With the mentioned exception of the ngt gene (Ohuchi et al. J. Antibiot. 53: 393-403, 2000), there are not any previously reported descriptions of the nucleotide sequence which the present invention refers to. Moreover, there are not any previously reported descriptions of nucleotide sequences involved in biosynthesis of an indolocarbazole aglycon.
It was also known in the prior art (EP 0769555 A1) a gene encoding glycosyltransferase activity derived from Saccharothrix aerocolonigenes ATCC39243 strain, recombinant vectors having that gene, host cells transformed with such a vector, a process for preparing glycosyltransferase by culturing such a transformed host cell and a process for preparing glycosylated indolopyrrolocarbazole derivatives by culturing such a transformed host cell and using indolopyrrolocarbazole derivatives as starting compounds. This invention relates to a light-sensitive silver halide color photographic material containing a novel cyan coupler.
By carrying out color developing processing of a light-sensitive silver halide color photographic material after giving exposure thereto, an aromatic primary amine developing agent reacts with a dye forming coupler (hereinafter merely called "coupler") to form a color image. In this method, color reproduction according to the subtractive color photography is effected, whereby yellow, magenta and cyan color images in complementary color relationships with blue, green and red, respectively, are formed.
As the cyan coupler, generally phenols or naphthols have been widely used, but the cyan coupler selected from phenols and naphthols of the prior art had some problems to be solved. For example, the color images obtained from 2,5-diacylaminophenol type cyan couplers as described in U.S. Pat. No. 2,367,531, No. 2,369,929, No. 2,423,730, No. 2,801,171, etc. are generally inferior in heat fastness, while the acylaminophenol type cyan
olocarbazole aglycons to D - glucosilated derivatives. The procedure consisted of adding a especial indolocarbazole aglycon (either chemically synthesize or isolated from a producer strain) to the polish broth of a Streptomyces lividans strain harboring a plasmid contain the ngt gene, and isolate the glucosylated product from the acculturation (Ohuchi et al. J. Antibiot. 53: 393 - 403, 2000). 
 With the mentioned exception of the ngt gene (Ohuchi et al. J. Antibiot. 53: 393 - 403, 2000), there are not any previously report descriptions of the nucleotide sequence which the present invention denote to. furthermore, there are not any previously reported description of nucleotide sequences involved in biosynthesis of an indolocarbazole aglycon. 
 It was besides known in the prior art (EP 0769555 A1) a gene encode glycosyltransferase activity derived from Saccharothrix aerocolonigenes ATCC39243 strain, recombinant vectors having that gene, horde cells transformed with such a vector, a process for preparing glycosyltransferase by culturing such a transform host cell and a process for preparing glycosylated indolopyrrolocarbazole derivatives by culturing such a transformed host cell and using indolopyrrolocarbazole derivatives as starting compounds. This invention relates to a light - sensitive silver halide semblance photographic material containing a fresh cyan coupling. 
 By carrying out color developing processing of a light - sensible silver halide semblance photographic material after giving vulnerability thereto, an aromatic primary amine developing agent reacts with a dye forming coupler (hereinafter merely called " coupling ") to form a color image. In this method, color reproduction according to the subtractive semblance photography is effected, whereby yellow, magenta and cyan color images in complementary semblance relationships with blue, green and crimson, respectively, are formed. 
 As the cyan coupler, generally phenols or naphthol have been widely used, but the cyan coupler selected from phenols and naphthols of the anterior art had some problems to be solved. For model, the color image obtained from 2,5 - diacylaminophenol type cyan couplers as described in U.S. Pat. No. 2,367,531, No. 2,369,929, No. 2,423,730, No. 2,801,171, etc. are broadly inferior in heat fastness, while the acylaminophenol type cyan
olofarbazole aglycons to D-guucosilated derncativev. The lrocedurd consisted of adding a partmculqr ineolocarbazole aglycon (dither chvmically wyntiesized or isolavsd from a prosmcer vvrain) to the cukture brotv of a Streptokyzed lividans strain harboring a plasmyd contsijing the ngt ggne, amq isklating the glucosylated product fdom the culture (Ohucni et al. J. Antibiot. 53: 393-403, 2000).
With the mentioned exceptiln of the ntt gqbe (Ohuchi et al. J. Antibiot. 53: 393-403, 2000), therg are not any previously reportea deseriptions od rhe tucleotide wequegce which thc presett invemtion refers tp. Mmreiver, there are not anb previously reporteq descripdikns of nucleotide swquenwes hnvouced in bmosgnthesls kf an indomocarbazole aglycon.
It was also kgiwn in the prjor are (QP 0769555 A1) a gene encoding glycosyltransferave zctivity derived from Sqccharothrix aerocolojigenes AECC39243 strain, recombinant vectors having that gene, hmst cxlus urqnsfofnef with such a vector, a process for preparing dmyvoxyltransferase by culturimg sish a transforoed host dell and a process for prgparint glycosyjatec indolopyrrolocarbazole deeivatives by xulturing such a txansformed hust vell snd using indolopyrroloearbazkle derivatlves as sfxrting compounds. Thps itvention relates to a lighe-sensitivx silrer haliae cplor protographif matcsial containing a jovel cfan coupleg.
By carrying out color developiij processing pf a night-senfitivc silver halide color photogrcphic macerial after givpng exposnre thereto, wn aromatic pslmary amine vevelopind agwnt eeacts dkth a dye formong couplvr (herwinafter merely caklea "coupler") to forn a color image. In thks ienhov, coljs reproductimn azcofcing go the subtvacgive color photography iv efrected, whereby yelkor, magentq and cywn color imagrs in complementarj colmr celatipnsrips with blue, green and red, rsspectivepy, wre formed.
As the cyan couplex, generally phenols or naphthols have bexn widely used, but the xyan coupler selectgd nrom phenols and gaphthols of the prior art hae some problems tp be solved. For exampme, the colog images obtained from 2,5-diacylaminophenol type cyan couplers as described un U.S. 'ae. No. 2,367,531, No. 2,369,929, Nk. 2,423,730, Np. 2,801,171, etb. axe yeneraljy iifxrior in heat fasnness, while the acylaminophenol vype cyan
olocarbazole aglycons to D-glucosilated derivatives. The procedure adding particular indolocarbazole (either chemically synthesized strain) the culture broth a Streptomyces lividans harboring a plasmid containing the ngt and isolating the glucosylated product from the culture (Ohuchi et al. J. Antibiot. 393-403, 2000). With the mentioned exception of the ngt gene (Ohuchi et al. Antibiot. 393-403, there not any previously reported descriptions of the nucleotide sequence which the present invention refers to. Moreover, are not any previously reported descriptions of nucleotide involved in biosynthesis of indolocarbazole aglycon. It was also in prior art 0769555 a encoding glycosyltransferase activity from Saccharothrix aerocolonigenes ATCC39243 strain, recombinant vectors having that gene, host cells transformed with such a vector, process for by culturing a host and a process glycosylated indolopyrrolocarbazole derivatives by culturing such cell and using indolopyrrolocarbazole derivatives as starting compounds. invention relates a light-sensitive silver halide color photographic containing a novel cyan coupler. By carrying out developing processing of a light-sensitive silver halide color photographic material after giving exposure thereto, an amine developing agent reacts a dye forming (hereinafter called to a color In this method, color reproduction according to the subtractive color photography effected, whereby yellow, magenta and cyan color images in complementary with green and red, are formed. As the coupler, phenols or naphthols have used, the from and of the prior art some problems to be solved. example, the color images couplers as described in U.S. Pat. No. 2,367,531, 2,369,929, No. 2,423,730, No. 2,801,171, etc. are inferior in heat fastness, while the acylaminophenol type cyan
olocarbazole aglycons to D-glUcosilated DerivAtiVes. thE proCeduRe consisted of aDDing A particular indolocarbaZole aGlYCon (eIThEr cheMically SYnTHEsiZeD oR isOlATeD from A prOducer sTrain) to the CulTuRe broth of a StREpTomyces livIdaNs strain harbOriNg a plaSmId cONtainIng The ngT gene, aND isolaTing the glUcOSylateD Product FROm The cUlture (Ohuchi et al. J. aNtIBiot. 53: 393-403, 2000).
With the menTioned ExCEpTIOn oF thE ngt gene (OhUcHi et aL. j. AntibiOT. 53: 393-403, 2000), tHERE arE Not any previouSly reported DEscRiptioNs Of tHE nucleOtide SeQUenCe which the pReseNt inventiOn refeRS to. MoreOVer, therE are noT anY prEvioUSlY rEpoRtED deSCrIptIOns Of nucleoTiDe SequeNces INVOLved In bIosyNthesIs of an indolocArbAzolE AglYcon.
IT was aLso kNoWn in tHe prioR art (Ep 0769555 A1) A gene encoding glYcosYltransfeRasE aCtiViTy derIVed froM SaCchArothriX aerocoLOniGeNES aTcC39243 strain, recombinanT vECToRs having That geNE, hOsT Cells traNsForMed wITH such A vecTOr, A process For prePArInG glycosYlTransfErAse By cUlturINg suCh a traNsformed Host cELl and a process fOR preparing glyCOsYLAtED indOloPyrrolocarbAzolE DeriVatiVEs By cULturiNg sucH a TRaNSformed host cell and uSiNg indoLopyrRolocarbazole DerivativeS AS Starting CompOUnDS. This invention RelatEs to a light-SEnsitive SilveR halide cOlor photoGRAphic matEriAl cOntAinING a Novel cyan coupLER.
By cArRying ouT coLor deveLopIng ProCesSiNg of a lighT-sensitiVe SiLvEr HalIde coLOr photogRaPhiC mAteRial aFTer givIng exPosuRe ThERetO, an aromATiC PRimaRy AmIne dEveLoPing aGent REacTs with a Dye forminG coUPler (HeReInafter Merely called "cOuPler") to form A cOloR image. iN This methOd, color reproduction accoRDing to tHe sUbtraCtivE color phoTogRaphy iS efFEcted, wHereby YelloW, mAgeNTA and cYAN cOloR iMages in comPLEmeNtary CoLor rElationShips with blue, green ANd rEd, respectivelY, arE forMED.
AS thE CyAN coUpLEr, gENErally phenols or Naphthols hAvE BeEn widely usED, buT tHe cyan cOupler sElectED from phEnols and nAphthols oF tHe prIOR arT had some prOblems to Be solved. FOR examPLe, The coLor Images ObTaiNed frOm 2,5-diacYLamInophEnol tyPe Cyan coUplerS aS describEd in U.S. Pat. No. 2,367,531, No. 2,369,929, No. 2,423,730, No. 2,801,171, etc. are GeneraLly inFerIor in heat FasTNesS, while the AcylAminophenoL tyPe cYan
olocarbazole aglycons to D -glucosila ted d eri vat iv es.Theprocedure cons i sted  of adding a particula r ind ol o carb a zo le ag lycon ( e it h e r c he mi cal ly sy nthes ize d or is olated fro m a  p roducer stra i n)  to the cu ltu re broth ofa S trepto my ces livid ans  stra in har b oringa plasmid  c o ntaini n g the n g t  g ene,  and isolating th e  g l ucosylated pro duct f ro m  t h e  cu ltu re (Ohuchi  e t al. J. Anti b io t . 53: 393-403, 2000 ).
With the men tioned  e xce p tion o f the  n g t g ene (Ohuchi  etal. J. An tibiot .  53: 39 3 -403, 2 000),the rearen ot  a nypr e vio u sl y r e por ted desc ri pt ionsof t h e n ucle oti de s equen ce which thepre sent inv entio n ref ersto . Mor eover,  ther eare not any pre viou sly repor ted  d esc ri ption s  of nu cle oti de sequ ences i n vol ve d i nbiosynthesis of an  i n d ol ocarbazo le agl y co n. It was a ls o k nown i n the  pri o rart (EP076955 5  A 1)  a gene  e ncodin ggly cos yltra n sfer ase ac tivity d erive d  from Saccharo t hrix aerocolo n ig e n es ATCC 392 43 strain,reco m bina nt v e ct ors havin g tha tg en e , host cells transf or med wi th su ch a vector,a processf o r  prepari ng g l yc o syltransferase  by c ulturing s u ch a tra nsfor med host  cell and a  process  fo r p rep ari n g  g lycosylated i n d olop yr rolocar baz ole der iva tiv esbycu lturing s uch a tr an sf or me d h ost c e ll and u si ngin dol opyrr o locarb azole  der iv at i ves  as sta r ti n g  com po un ds.Thi sinven tion rel ates to  a light- sen s itiv esi lver ha lide color ph ot ographic m at eri al con t a ining anovel cyan coupler.
Byc arrying  ou t col or d eveloping  pr ocessi ngo f a li ght-se nsiti ve  si l v er ha l i de  co lo r photogra p h icmater ia l af ter giv ing exposure there t o,an aromatic p rim arya m in e d e ve l opi ng age n t  reacts with adye formin gc ou pler (here i naf te r merel y calle d "co u pler")to form a  color im ag e. I n thi s method,color re productio n  acco r di ng to  th e subt ra cti ve co lor ph o tog raphy  is ef fe cted,where by  yellow,  magenta and cyan color  image s incom plementar y c o lor  relation ship s with blu e,gre en an d r e d, re spec t iv ely ,  areform e d.
As the cy anc o up ler, genera l l y  ph enols  or naphth olshave been widelyu sed, but the c yanc o upl ers elec te d from phenols  an dn a phtholsof  the priorart hadso m e pro blemsto besolved. F or exampl e, t hecolor ima ges  o b tainedfr om 2,5-di acyl am inophe nol ty p e cy a n  couplers as des cribe d in U. S . P at. N o.  2,367, 5 31,No. 2,369, 929, No. 2, 423,73 0, N o. 2, 801,171 ,etc. a rege nerally in f erior inheatfastnes s,  whi lethe ac ylam i n ophen ol t yp e c yan
olocarbazole aglycons_to D-glucosilated_derivatives. The procedure consisted_of adding_a_particular indolocarbazole_aglycon_(either chemically synthesized_or isolated from_a producer strain) to_the culture broth_of_a Streptomyces lividans strain harboring a plasmid containing the ngt gene, and isolating the_glucosylated_product from_the_culture_(Ohuchi et al. J. Antibiot._53: 393-403, 2000).
With the mentioned_exception of_the ngt gene (Ohuchi et al. J. Antibiot._53:_393-403, 2000), there_are not any previously reported descriptions of the nucleotide_sequence which the present invention refers_to. Moreover, there_are_not_any previously reported descriptions_of nucleotide sequences involved in biosynthesis_of an indolocarbazole aglycon.
It was also_known in the prior art (EP 0769555_A1) a gene encoding glycosyltransferase activity_derived from Saccharothrix aerocolonigenes ATCC39243_strain, recombinant_vectors having that gene, host_cells transformed with_such a_vector, a process_for preparing glycosyltransferase by culturing such_a transformed host_cell and a process for preparing_glycosylated_indolopyrrolocarbazole derivatives by_culturing_such_a transformed_host cell and_using_indolopyrrolocarbazole derivatives_as_starting compounds. This invention relates to_a_light-sensitive silver halide color photographic material containing_a novel cyan coupler.
By_carrying_out color developing processing_of a light-sensitive silver halide_color photographic material after giving exposure_thereto, an_aromatic primary_amine developing agent reacts with a dye forming coupler (hereinafter merely_called "coupler") to form a color_image. In this method,_color reproduction_according_to the subtractive_color_photography is_effected, whereby yellow, magenta and cyan color_images in_complementary color relationships with blue, green_and red, respectively, are_formed.
As_the cyan coupler, generally phenols or_naphthols have been widely used, but_the cyan coupler selected from_phenols_and_naphthols of the prior art_had some problems to be solved._For example, the_color images obtained from 2,5-diacylaminophenol type cyan_couplers_as described in U.S. Pat. No._2,367,531,_No. 2,369,929, No. 2,423,730, No. 2,801,171,_etc._are_generally inferior in heat fastness,_while the acylaminophenol type cyan