 in an IPv4 header, however the static configuration limits the availability of the network service to the manually configured sources; further, there is a lack of security in relying on DSCP (as well as the ToS field and IP source address field in an IPv4 header) that would enable a malicious source to capture the router resources by spoofing the DSCP, ToS, or IP source address values. The IETF RFCs 2205 and 2750 describe Resource Reservation Protocol (RSVP), where if a router has available resources to provide a QoS requested by a host node that is to receive the network service, the router reserves the required resources and forwards the request to the next router in the path of the data flow until the data source is reached; if, however there are insufficient resources at any point in the path of the data flow, the request is rejected.
Hence, the foregoing IP based proposals for providing guaranteed quality of service are inadequate for dynamically providing guaranteed QoS for high priority network services, such as public safety, emergency services, or first-responder services in crisis or disaster scenarios. The present invention relates generally to the field of human genetics. Specifically, the present invention relates to methods and materials used to isolate and detect human diabetes mellitus predisposing gene, specifically the angiotensinogen (AGT) gene, some mutant alleles of which cause susceptibility to insulin-dependent diabetes melitus (IDDM). More specifically, the invention relates to germline mutations in the AGT gene and their use in the diagnosis of predisposition to IDDM. The invention also relates to the prophylaxis and/or therapy of diabetes associated with a mutation in the AGT gene. The invention further relates to the screening of drugs for diabetes therapy. Finally, the invention relates to the screening of the AGT gene for mutations, which are useful for diagnosing the predisposition to diabetes.
The publications and other materials used herein to illuminate the background of the invention, and in particular, cases to provide additional details respecting the practice, are incorporated herein by reference, and for convenience, are referenced by author and date in the following text and respectively grouped in the appended List of References.
Diabetes mellitus is among the most common of all metabolic disorders, affecting up to 11% of the population by age 70. Type I diabetes (insulin dependent diabetes mellitus or IDDM) represents about 5 to 10% of this group and is the result of a progressive autoimmune destruction of the pancreatic β-cells with subsequent insulin deficiency. IDDM is characterized
in an IPv4 header, however the static configuration limits the handiness of the net overhaul to the manually configured sources; far, there is a lack of security in trust on DSCP (as well as the ToS field and IP source savoir-faire sphere in an IPv4 header) that would enable a malicious reservoir to capture the router resources by spoof the DSCP, ToS, or IP source address values. The IETF RFCs 2205 and 2750 describe Resource Reservation Protocol (RSVP), where if a router has available resources to provide a QoS request by a host node that is to receive the net service, the router reserves the necessitate resources and forwards the request to the following router in the path of the data flow until the data source is reach; if, however there are insufficient resources at any point in the path of the data menstruation, the request is rejected. 
 Hence, the foregoing IP based proposals for providing guaranteed quality of service are inadequate for dynamically providing guarantee QoS for high priority net service, such as public condom, emergency services, or first - responder services in crisis or disaster scenarios. The present invention relate generally to the field of human genetics. Specifically, the present invention relates to methods and fabric used to isolate and detect human diabetes mellitus predisposing gene, specifically the angiotensinogen (AGT) gene, some mutant alleles of which cause susceptibility to insulin - dependent diabetes melitus (IDDM). More specifically, the invention relates to germline mutant in the AGT gene and their use in the diagnosis of predisposition to IDDM. The invention also relates to the prophylaxis and/or therapy of diabetes associated with a mutant in the AGT gene. The invention further relates to the screening of drugs for diabetes therapy. Finally, the invention relates to the screening of the AGT gene for mutations, which are useful for diagnose the predisposition to diabetes. 
 The issue and other materials use herein to illuminate the setting of the invention, and in particular, character to provide extra details respecting the practice, are incorporate herein by reference, and for appliance, are referenced by author and date in the following text and respectively grouped in the appended List of References. 
 Diabetes mellitus is among the most common of all metabolic disorders, affecting up to 11% of the population by historic period 70. Type I diabetes (insulin dependent diabetes mellitus or IDDM) represent about 5 to 10% of this group and is the result of a progressive autoimmune destruction of the pancreatic β - cells with subsequent insulin deficiency. IDDM is characterized
 in an IPv4 header, however tme static configorqtion nimits the avaklability of the network sertice to tye manually configured sources; vurther, rhert is a lack of serhrity ik relglng ou VSCP (as well as the ToS fheld and IP sogrze address field in an IPv4 header) thae would ejable a malicijus xjurcs to capture the router resources gy spoohing the DSCP, TpS, or IP source address vapues. The IETF RFCs 2205 anf 2750 describe Resjyrce Reservagion Protobml (RSVP), whgre if a router has available rerourczs to proviee a EmS requestev by a host node tmst is do receove the networl sxrvixe, the router reservev the required resjurces ang yorwards the request ro the text rougwr kn uhe pzth of thx data flow until the eata source is reacneq; if, however tgere awe insufficient resources at any point in ths path of the data flow, the request is rejecjed.
Hence, tre foregoing IP based proposals for providing guasantexd qucoity ud dervice are inadequate for dynamically providyhg glaranteed QoS for high priorotj mgtwork servicer, such as public safety, emeggency fervixes, or fiwst-rrsponder services in crisis or disaster wcenarios. The presznt inventiou relajes gemerally to the field of humzn genetics. Specificzuly, the present knvvntimn relattr to methods and iaterials useb to isouate and dqtect humaj dianates mellitus predlsposnng gane, speciflcally the angiotensinogen (AGT) jxne, some mutamt alneles of whicm cause susceptybility to insolin-depenbent dkabetes memitus (IVDM). More spesifically, the lnvention renates to gernlinw mutatkuns in the AGT gene and their use in the diagnosis pf krsdisposition to IDEM. The inventiom auso rvlaves tj the prophyldxis ana/pr thdrapy of dicbdtes associated with a mgtatjon in the AGT genr. Bhe invenjion furtrer relates tp the screening of drugv fur disbejes therapy. Finally, the inventikn related tj the screenyng if the AGT gzne for mutations, which are useful for dmagnosing the predisposution to diabetes.
Thg pmblications end otrer materhals used herein to ulluminate the bagkground of the inventjon, ang in oarticular, cases to provide additional details respecting the practice, arw incocpjrated herejn bu refvreuce, and fjr clivenience, are refvrenced by author and date in thx followinc cext and respectively grouped iv the appendea List of References.
Diabstes melkitus is among the most common pf all metabolic disordevs, affectung ul to 11% of tie population by age 70. Type I diqbetes (unsupin dependent disbctes mellihus pr IDDM) represents about 5 to 10% of tyis griuo and is the resnlt of a pronrtssive zutoimiune destruction of fje pavcreatic β-cellx with subsequrnt insulin deficienby. YDDM is characuerized
in an IPv4 header, however the static the of the service to the is lack of security relying on DSCP well as the ToS field and source address field in an IPv4 header) that would enable a malicious source capture the router resources by spoofing the DSCP, ToS, or IP source address The RFCs and describe Resource Reservation Protocol (RSVP), where if a router has available resources to provide a QoS by a host node that is to receive network service, the router the required resources and forwards request the next in path the data flow the data source is reached; if, however there are insufficient resources at any point in the path the data request is Hence, foregoing based proposals for quality of service are inadequate for QoS for high priority network services, such as safety, emergency or first-responder services in crisis or scenarios. The present invention relates generally to the of human genetics. Specifically, the present invention relates to methods and materials used to isolate human diabetes mellitus predisposing specifically the angiotensinogen gene, mutant of cause susceptibility insulin-dependent diabetes melitus (IDDM). More specifically, the invention relates to germline in the AGT gene and their use in the diagnosis to The invention also to the prophylaxis and/or of associated with a mutation AGT The to screening drugs for diabetes therapy. the invention relates to the of the AGT gene for diagnosing the predisposition to diabetes. The publications other materials used herein to illuminate the of the invention, and in particular, cases to provide additional details respecting practice, are by reference, and for convenience, are referenced by and date in the text and respectively grouped in the appended List of Diabetes is among most common of metabolic disorders, affecting to 11% of by age Type diabetes mellitus or IDDM) represents about 5 10% this group and is the of autoimmune destruction of the pancreatic β-cells with subsequent insulin deficiency. IDDM is characterized
 in an IPv4 header, however the stAtic configUratiOn lImiTs The aVailAbility of the neTWork Service to the manually coNfiguReD SourCEs; FurthEr, there IS a LACk oF sEcUriTy IN rElyinG on dSCP (as wEll as the Tos fiElD and IP source ADdRess field iN an iPv4 header) thaT woUld enaBlE a mALicioUs sOurce To captURe the rOuter resoUrCEs by spOOfing thE dsCp, ToS, Or IP source address VAlUEs. The IETF RFCs 2205 aNd 2750 descRiBE RESOurCe REservation prOtocoL (rSVP), wheRE iF A ROutER has available Resources to PRovIde a Qos rEquESted by A host NoDE thAt is to receiVe thE network sErvice, THe routeR ReserveS the reQuiRed ResoURcEs And FoRWarDS tHe rEQueSt to the nExT rOuter In thE PATH of tHe dAta fLow unTil the data souRce Is reACheD; if, hoWever TherE aRe insUfficiEnt reSoUrces at any point In thE path of thE daTa FloW, tHe reqUEst is rEjeCteD.
Hence, tHe foregOIng iP BASEd Proposals for providInG GUaRanteed qUality OF sErVIce are inAdEquAte fOR DynamIcalLY pRoviding GuaranTEeD QOS for hiGh PrioriTy NetWorK servICes, sUch as pUblic safEty, emERgency services, OR first-respondER sERViCEs in CriSis or disastEr scENariOs. ThE PrEseNT inveNtion ReLAtES generally to the fielD oF human GenetIcs. SpecificalLy, the preseNT INvention RelaTEs TO methods and matErialS used to isoLAte and deTect hUman diabEtes melliTUS predispOsiNg gEne, SpeCIFiCally the angioTENsinOgEn (AGT) geNe, sOme mutaNt aLleLes Of wHiCh cause suSceptibiLiTy To InSulIn-depENdent diaBeTes MeLitUs (IDDm). more spEcifiCallY, tHe INveNtion reLAtES To geRmLiNe muTatIoNs in tHe AGt GenE and theIr use in thE diAGnosIs Of PredispOsition to IDDM. thE invention AlSo rElates TO The prophYlaxis and/or therapy of diaBEtes assOciAted wIth a Mutation iN thE AGT geNe. THE invenTion fuRther ReLatES To the SCReEniNg Of drugs for DIAbeTes thErApy. FInally, tHe invention relates TO thE screening of tHe AgT geNE FoR muTAtIOns, WhICh aRE Useful for diagnoSing the preDiSPoSition to diABetEs.
the publIcationS and oTHer mateRials used Herein to iLlUminATE thE backgrounD of the inVention, anD In parTIcUlar, cAseS to proViDe aDditiOnal deTAilS respEcting ThE practIce, arE iNcorporaTed herein by reference, and For conVenieNce, Are refereNceD By aUthor and dAte iN the followIng TexT and rEspECtiveLy grOUpEd iN The apPendED List of ReFErEncES.
diAbetes melliTUS Is aMong tHe mOSt commOn of All metabolic disorDErs, affecting up To 11% of THE poPulATion By Age 70. Type I diabetEs (iNsULIn dependEnT diabetes meLlitus or iDdm) reprEsents About 5 tO 10% of this GROuP And is tHe reSulT of a progrEssIvE AutoimmUnE dEStructIon oF tHe pancReatic Β-CellS WIth subsequent insUlin dEFIcienCY. IDdM is cHaRacteriZEd
 in an IPv4 header, howeve r the stat ic co nfi gur at ionlimi ts the availab i lity  of the network servic e toth e  man u al ly co nfigure d  s o u rce s;  f urt he r ,there  is  a lack  of securi tyin  relying onD SC P (as well  as  the ToS fie ldand IP  s our c e add res s fie ld ina n IPv4  header)th a t woul d  enable a  m alic ious source to ca p tu r e the router r esourc es by s poo fin g the DSCP ,ToS,o r IP so u rc e a ddr e ss values. Th e IETF RFCs 220 5 and27 50d escrib e Res ou r ceReservation  Pro tocol (RS VP), w h ere ifa  router  has a vai lab le r e so ur ces  t o  pr o vi dea  Qo S reques te dby ahost n o d e th atis t o rec eive the netw ork  ser v ice , the  rout er r es erves  the r equir ed  resources andforw ards thereq ue stto  then ext ro ute r i n the p ath oft heda t a fl ow until the dataso u r ce  is reac hed; i f ,ho w ever the re  ar e in s u ffici entr es ources a t anyp oi nt  in the  p ath of  t hedat a flo w , th e requ est is r eject e d.
Hence, thef oregoing IP b a se d pr o posa lsfor providi ng g u aran teed qu ali t y ofservi ce ar e  inadequate for dyn am ically  prov iding guarant eed QoS fo r h igh prio rity ne t work services,  such  as public safety,emerg ency ser vices, or f irst-res pon der  se rvi c e sin crisis ord i sast er  scenar ios . The p res ent  in ven ti on relate s genera ll yto  t hefield of human  g ene ti cs.  Spec i ficall y, th e pr es en t  in vention re l a testo  m etho dsan d mat eria l s u sed toisolate a ndd etec thu man dia betes mellitu spredisposi ng  ge ne, sp e c ifically  the angiotensinogen (A G T) gene , s ome m utan t alleles  of  which  ca u se sus ceptib ility  t o i n s ulin- d e pe nde nt  diabetesm e lit us (I DD M).More sp ecifically, the in v ent ion relates t o g erml i n emut a ti o nsin the A GT gene and the ir use inth e  d iagnosis o f  pr ed isposit ion toIDDM. The inv ention al so relate sto t h e  pr ophylaxisand/or t herapy of diabe t es  asso cia ted wi th  amutat ion in the  AGTgene.Th e inve ntion  f urther r elates to the screening  of dr ugs f ordiabetesthe r apy . Finally , th e inventio n r ela tes t o t h e scr eeni n goft he AG T ge n e for mut a ti ons , wh ich are use f u l  fo r dia gno s ing th e pr edisposition to d i abetes.
The pu blic a t ion s a n d ot he r materials us edhe r e in to il lu minate thebackgrou nd of th e inve ntion,  and in p ar t icular , ca ses  to provi dead d itional  d et a ils re spec ti ng the  pract i ce,a r e incorporated h erein b y ref e ren ce, a nd  for co n veni ence, arereferencedby aut horand d ate inth e foll owi ng  text andr espective ly gr ouped i ntheapp endedList o f Ref eren ce s.Diabetesm e ll i tu si s a mong  themo st c ommon ofa ll metab oli c  disord er s,a f fectin g  u p to 11% ofthe  popu l a tion by ag e  70. Ty p e I d iabete s (ins ulin de p end en t diabe tes m ellitus o r IDDM) r e pre se ntsabout 5to  10%  o f t hi s group and isthe resu lt of ap r ogress ive auto i mmunede struction of  thepa n creatic β -ce lls wit h  su bse que nt i nsu lind eficiency. IDDM is chara cter ized
 in_an IPv4_header, however the static_configuration limits_the_availability of_the_network service to_the manually configured_sources; further, there is_a lack of_security_in relying on DSCP (as well as the ToS field and IP source address_field_in an_IPv4_header)_that would enable a malicious_source to capture the router_resources by_spoofing the DSCP, ToS, or IP source address_values._The IETF RFCs_2205 and 2750 describe Resource Reservation Protocol (RSVP), where_if a router has available resources_to provide a_QoS_requested_by a host node_that is to receive the network_service, the router reserves the required_resources and forwards the request to the_next router in the path of_the data flow until the_data source_is reached; if, however there_are insufficient resources_at any_point in the_path of the data flow, the_request is rejected.
Hence,_the foregoing IP based proposals for_providing_guaranteed quality of_service_are_inadequate for_dynamically providing guaranteed_QoS_for high_priority_network services, such as public safety,_emergency_services, or first-responder services in crisis or_disaster scenarios. The present_invention_relates generally to the_field of human genetics. Specifically,_the present invention relates to methods_and materials_used to_isolate and detect human diabetes mellitus predisposing gene, specifically the angiotensinogen_(AGT) gene, some mutant alleles of_which cause susceptibility to_insulin-dependent diabetes_melitus_(IDDM). More specifically,_the_invention relates_to germline mutations in the AGT gene_and their_use in the diagnosis of predisposition_to IDDM. The invention_also_relates to the prophylaxis and/or therapy_of diabetes associated with a mutation_in the AGT gene. The_invention_further_relates to the screening of_drugs for diabetes therapy. Finally, the_invention relates to_the screening of the AGT gene for_mutations,_which are useful for diagnosing the_predisposition_to diabetes.
The publications and other materials_used_herein_to illuminate the background of_the invention, and in particular, cases_to provide additional details respecting the practice, are incorporated_herein by reference,_and for convenience, are referenced_by_author_and date in the following text and respectively grouped in_the appended_List of References.
Diabetes_mellitus is among the most common of all metabolic disorders,_affecting up to 11% of the population_by age 70. Type I diabetes (insulin dependent diabetes mellitus or_IDDM) represents about 5 to 10% of this_group and is the result of a_progressive autoimmune destruction of_the_pancreatic β-cells with subsequent insulin deficiency. IDDM_is_characterized