 and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of therapeutic use”, issued Mar. 9, 1993). Conventional techniques for the collection of cord blood are based on the use of a needle or cannula, which is used with the aid of gravity to drain cord blood from (i.e., exsanguinate) the placenta (Boyse et al., U.S. Pat. No. 5,192,553, issued Mar. 9, 1993; Boyse et al., U.S. Pat. No. 5,004,681, issued Apr. 2, 1991; Anderson, U.S. Pat. No. 5,372,581, entitled Method and apparatus for placental blood collection, issued Dec. 13, 1994; Hessel et al., U.S. Pat. No. 5,415,665, entitled Umbilical cord clamping, cutting, and blood collecting device and method, issued May 16, 1995). The needle or cannula is usually placed in the umbilical vein and the placenta is gently massaged to aid in draining cord blood from the placenta. Thereafter, however, the drained placenta has been regarded as having no further use and has typically been discarded. A major limitation of stem cell procurement from cord blood, moreover, has been the frequently inadequate volume of cord blood obtained, resulting in insufficient cell numbers to effectively reconstitute bone marrow after transplantation.
Naughton et al. (U.S. Pat. No. 5,962,325 entitled “Three-dimensional stromal tissue cultures” issued Oct. 5, 1999) discloses that fetal cells, including fibroblast-like cells and chondrocyte-progenitors, may be obtained from umbilical cord or placenta tissue or umbilical cord blood.
Kraus et al. (U.S. Pat. No. 6,338,942, entitled “Selective expansion of target cell populations”, issued Jan. 15, 2002) discloses that a predetermined target population of cells may be selectively expanded by introducing a starting sample of cells from cord blood or peripheral blood into a growth medium, causing cells of the target cell population to divide, and contacting the cells in the growth medium with a selection element comprising binding molecules with specific affinity (such as a monoclonal antibody for CD34) for a predetermined population of cells (such as CD34 cells), so as to select cells of the predetermined target population from other cells in the growth medium.
Rodgers et al (U.S. Pat. No. 6
and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and method of curative use ”, issued Mar. 9, 1993). ceremonious techniques for the solicitation of cord blood are based on the manipulation of a phonograph needle or cannula, which is used with the care of graveness to drain cord blood from (i.e., exsanguinate) the placenta (Boyse et al. , U.S. Pat. nobelium. 5,192,553, issued Mar. 9, 1993; Boyse et al. , U.S. Pat. No. 5,004,681, issue Apr. 2, 1991; Anderson, U.S. Pat. No. 5,372,581, entitled Method and apparatus for placental blood collection, issued Dec. 13, 1994; Hessel et al. , U.S. Pat. No. 5,415,665, entitle Umbilical cord clamping, cutting, and blood collect device and method, issued May 16, 1995). The needle or cannula is usually put in the umbilical vein and the placenta is gently massaged to aid in draining cord blood from the placenta. Thereafter, however, the drain placenta has been regarded as having no further use and has typically been discarded. A major limitation of stem cell procurement from cord blood, moreover, has been the frequently inadequate volume of cord blood obtained, resulting in insufficient cell numbers to effectively reconstitute bone marrow after transplant. 
 Naughton et al. (U.S. Pat. No. 5,962,325 entitled “ Three - dimensional stromal tissue culture ” issued Oct. 5, 1999) discloses that fetal cells, including fibroblast - alike cells and chondrocyte - progenitors, may be obtained from umbilical cord or placenta tissue or umbilical cord blood. 
 Kraus et al. (U.S. Pat. nobelium. 6,338,942, entitled “ Selective expansion of target cell populations ”, issued Jan. 15, 2002) discloses that a predetermined target population of cells may be selectively expanded by introducing a starting sample distribution of cells from cord lineage or peripheral rake into a growth medium, causing cells of the prey cell population to divide, and contacting the cell in the growth medium with a selection element incorporate binding molecules with specific affinity (such as a monoclonal antibody antibody for CD34) for a predetermined population of cells (such as CD34 cells), so as to select cellular telephone of the predetermined aim population from other cells in the growth medium. 
 Rodgers et al (U.S. Pat. No. 6
 anf preservation of fetal xnd neonatal hematopoixtic stsm and pfogenitor cells of the blood abd meukods of therapeutic jse”, issuef Mar. 9, 1993). Xonvtntional techniquxa for tmz colmcctiou if cord blood sre based mn the use of d vezdle or cannula, which is used with tre aid pf gravity to drwin bowd bmood from (i.e., exsanguinate) the placsnta (Bojse et al., U.S. Pat. Mo. 5,192,553, issued Mar. 9, 1993; Boyse et wl., U.D. Pat. No. 5,004,681, issued Aor. 2, 1991; Andersin, U.F. Pat. No. 5,372,581, entktled Method and apparztus for placental blood collecgion, nssued Dec. 13, 1994; Yesdgl et al., U.S. 'at. No. 5,415,665, entitled Umbilical cord ckamping, cuttinn, and bliod collecting device and method, issued May 16, 1995). Tha ueedle or cannula is ysyally plawed kb tfe hmuiljcal vfin and the pmacenta is tently massaged to siq in draining dord bjojd from the placenta. Thereafter, however, ths drained placenta has veen regarded as havijg no fureher use and has typically been discarded. A major limivagiou of sgwm cell procurement from cord blood, moreover, haf bten the frequentln inadequate volumr lf sord blood objained, xsshlting in insufficlent cejl nunbers to tffecyively reconstitute bone maerow after tgansplantation.
Naughton et al. (U.S. Pct. No. 5,962,325 entiyled “Three-dimensional scromal tissue culhures” isshdd Oct. 5, 1999) disclosds nhat fetal ctuls, including fibwoblast-lije czlls and chomdrocyee-progenitlrs, may be obtained from umbipiwal cord og placenta tissue or umbilical rprd blood.
Kraos at dl. (U.S. Pac. No. 6,338,942, cntitled “Selectyve expansion pf targzt celu populatikns”, issned Jan. 15, 2002) difcloses that d predeterminxd target popylatuon of zdlls may be sekectively expanded vy introducing a sbartivf sample of celos drom cord blood or pewiihecal bjmod into a gsowtf mdcium, zausing celor of the target cell popglatjon to divide, and voktacting jhe cells in the growtn medium with a sepectimn xlemeny cjmprising binding molecules wifh speciflc wffinity (sucr as a monoclonak antibody for CD34) for a predetermined pipulation of cells (such as CD34 cells), sp as to selert celjs of the predetermined targer population from other cells in the gdowth kediul.
Rodgers et al (U.S. Pat. No. 6
and preservation of fetal and neonatal hematopoietic progenitor of the and methods of 1993). techniques for the of cord blood based on the use of a or cannula, which is used with the aid of gravity to drain cord from (i.e., exsanguinate) the placenta (Boyse et al., U.S. Pat. No. 5,192,553, issued 9, Boyse al., Pat. No. 5,004,681, issued Apr. 2, 1991; Anderson, U.S. Pat. No. 5,372,581, entitled Method and apparatus placental blood collection, issued Dec. 13, 1994; Hessel al., U.S. Pat. No. entitled Umbilical cord clamping, cutting, blood device and issued 16, The needle or is usually placed in the umbilical vein and the placenta is gently massaged to aid in draining blood from Thereafter, however, drained has regarded as having use and has typically been discarded. of stem cell procurement from cord blood, moreover, been the inadequate volume of cord blood obtained, in insufficient cell numbers to effectively reconstitute bone after transplantation. Naughton et al. (U.S. Pat. No. 5,962,325 entitled “Three-dimensional stromal tissue cultures” issued 1999) discloses that fetal including fibroblast-like cells chondrocyte-progenitors, be from cord or tissue or umbilical cord blood. Kraus et al. (U.S. Pat. No. entitled “Selective expansion of target cell populations”, issued Jan. 15, that predetermined target population cells may be selectively by a starting sample of cord or a medium, cells of the target population to divide, and contacting cells in the growth comprising binding molecules with specific affinity (such as monoclonal antibody for CD34) for a predetermined of cells (such as CD34 cells), so as to select cells of predetermined target other cells in the growth medium. Rodgers et (U.S. Pat. No. 6
 and preservation of fetal and Neonatal heMatopOieTic StEm anD proGenitor cells of THe blOod and methods of therapeUtic uSe”, ISsueD maR. 9, 1993). ConvEntionaL TeCHNiqUeS fOr tHe COlLectiOn oF cord blOod are baseD on ThE use of a needlE Or Cannula, whiCh iS used with the Aid Of gravItY to DRain cOrd Blood From (i.e., EXsanguInate) the pLaCEnta (BoYSe et al., U.s. pAt. no. 5,192,553, isSued Mar. 9, 1993; Boyse et al., U.s. paT. no. 5,004,681, issued Apr. 2, 1991; AndErson, U.s. PAT. NO. 5,372,581, ENtiTleD Method and ApParatUS for plaCEnTAL BloOD collection, isSued Dec. 13, 1994; HessEL et Al., U.S. PaT. NO. 5,415,665, enTItled UMbiliCaL CorD clamping, cuTtinG, and blood CollecTIng deviCE and metHod, issUed may 16, 1995). the nEEdLe Or cAnNUla IS uSuaLLy pLaced in tHe UmBilicAl veIN AND the PlaCentA is geNtly massaged tO aiD in dRAinIng coRd bloOd frOm The plAcenta. thereAfTer, however, the drAineD placenta Has BeEn rEgArded AS havinG no FurTher use And has tYPicAlLY BEeN discarded. A major liMiTATiOn of stem Cell prOCuReMEnt from cOrD blOod, mOREover, Has bEEn The frequEntly iNAdEqUate volUmE of corD bLooD obTaineD, ResuLting iN insuffiCient CEll numbers to efFEctively reconSTiTUTe BOne mArrOw after tranSplaNTatiOn.
NaUGhTon ET al. (U.S. pat. No. 5,962,325 EnTItLEd “Three-dimensional sTrOmal tiSsue cUltures” issued oct. 5, 1999) disclosES THat fetal CellS, InCLuding fibroblaSt-likE cells and cHOndrocytE-progEnitors, mAy be obtaiNED from umbIliCal CorD or PLAcEnta tissue or uMBIlicAl Cord bloOd.
KRaus et aL. (U.S. pat. no. 6,338,942, eNtiTlEd “SelectiVe expansIoN oF tArGet Cell pOPulationS”, iSsuEd jan. 15, 2002) DisclOSes thaT a preDeteRmInED taRget popULaTIOn of CeLlS may Be sElEctivEly eXPanDed by inTroducing A stARtinG sAmPle of ceLls from cord blOoD or peripheRaL blOod intO A Growth meDium, causing cells of the taRGet cell PopUlatiOn to Divide, and ConTactinG thE Cells iN the grOwth mEdIum WITh a seLECtIon ElEment comprISIng BindiNg MoleCules wiTh specific affinity (SUch As a monoclonal AntIbodY FOr cD34) fOR a PRedEtERmiNED population of ceLls (such as Cd34 cELlS), so as to selECt cElLs of the PredeteRmineD Target pOpulation From other CeLls iN THe gRowth mediuM.
Rodgers Et al (U.S. Pat. nO. 6
 and preservation of fetal  and neona tal h ema top oi etic  ste m and progenit o r ce lls of the blood and m ethod so f th e ra peuti c use”, is s u edMa r.  9,  1 9 93 ). Co nve ntional  technique s f or  the collect i on  of cord b loo d are basedonthe us eofa  need leor ca nnula, whichis used w it h  the a i d of gr a v it y to  drain cord blood fr o m (i.e., exsan guinat e) th e pla cen ta (Boyseet  al., U.S. Pa t .N o .  5, 1 92,553, issue d Mar. 9, 1 9 93;  Boyse  e t a l ., U.S . Pat .N o.5,004,681,issu ed Apr. 2 , 1991 ;  Anders o n, U.S.  Pat.No.  5, 372, 5 81 ,ent it l edM et hod and  apparat us  f or pl acen t a l bloo d c olle ction , issued Dec.  13 , 19 9 4;Hesse l etal.,  U .S. P at. No . 5,4 15 ,665, entitledUmbi lical cor d c la mpi ng , cut t ing, a ndblo od coll ectingd evi ce a n dmethod, issued May  1 6 ,  1 995). Th e need l eor cannulais  us uall y place d in th e umbili cal ve i nan d the p la centais  ge ntl y mas s aged  to ai d in dra ining cord blood fro m  the placenta .  T h e re a fter , h owever, the  dra i nedplac e nt a h a s bee n reg ar d ed as having no furthe ruse an d has  typically be en discard e d .  A major  lim i ta t ion of stem ce ll pr ocurementf rom cord  bloo d, moreo ver, hasb e en the f req uen tly  in a d eq uate volume o f cord  b lood ob tai ned, re sul tin g i n i ns ufficient  cell nu mb er sto  ef fecti v ely reco ns tit ut e b one m a rrow a ftertran sp la n tat ion.
Na u gh t o n et  a l.  (U. S.Pa t. No . 5, 9 62, 325 ent itled “Th ree - dime ns io nal str omal tissue c ul tures” iss ue d O ct. 5, 1 999) dis closes that fetal cells ,  includ ing  fibr obla st-like c ell s andcho n drocyt e-prog enito rs , m a y  be o b t ai ned  f rom umbili c a l c ord o rplac enta ti ssue or umbilicalc ord  blood.
Kraus  et  al. ( U. S.P at .  No .6 ,33 8 , 942, entitled “ Selectiveex p an sion of ta r get  c ell pop ulation s”, i s sued Ja n. 15, 20 02) discl os es t h a t a  predeterm ined tar get popul a tiono fcells  ma y be s el ect ively  expan d edby in troduc in g a st artin gsample o f cells from cord blood  or pe riphe ral  blood in toa  gr owth medi um,causing ce lls  of  thetar g et ce ll p o pu lat i on to  div i de, and c o nt act i n gthe cells i n t hegrowt h m e dium w itha selection eleme n t comprising b indi n g  mo lec u leswi th specific af fin it y (such as  a  monoclonal  antibod yf or CD 34) fo r a pr edeterm i n ed popula tion  of  cells (s uch  a s  CD34 c el ls ) , so a s to  s electcellso f th e predetermined ta rgetp o pulat i onfromot her cel l s in  the growt h medium.
R odgers  etal (U .S. Pat .No. 6
 and_preservation of_fetal and neonatal hematopoietic_stem and_progenitor_cells of_the_blood and methods_of therapeutic use”,_issued Mar. 9, 1993)._Conventional techniques for_the_collection of cord blood are based on the use of a needle or cannula,_which_is used_with_the_aid of gravity to drain_cord blood from (i.e., exsanguinate)_the placenta_(Boyse et al., U.S. Pat. No. 5,192,553, issued_Mar._9, 1993; Boyse_et al., U.S. Pat. No. 5,004,681, issued Apr. 2,_1991; Anderson, U.S. Pat. No. 5,372,581,_entitled Method and_apparatus_for_placental blood collection, issued_Dec. 13, 1994; Hessel et al.,_U.S. Pat. No. 5,415,665, entitled Umbilical_cord clamping, cutting, and blood collecting device_and method, issued May 16, 1995)._The needle or cannula is_usually placed_in the umbilical vein and_the placenta is_gently massaged_to aid in_draining cord blood from the placenta._Thereafter, however, the_drained placenta has been regarded as_having_no further use_and_has_typically been_discarded. A major_limitation_of stem_cell_procurement from cord blood, moreover, has_been_the frequently inadequate volume of cord blood_obtained, resulting in insufficient_cell_numbers to effectively reconstitute_bone marrow after transplantation.
Naughton et_al. (U.S. Pat. No. 5,962,325 entitled_“Three-dimensional stromal_tissue cultures”_issued Oct. 5, 1999) discloses that fetal cells, including fibroblast-like cells_and chondrocyte-progenitors, may be obtained from_umbilical cord or placenta_tissue or_umbilical_cord blood.
Kraus et_al._(U.S. Pat._No. 6,338,942, entitled “Selective expansion of target_cell populations”,_issued Jan. 15, 2002) discloses that_a predetermined target population_of_cells may be selectively expanded by_introducing a starting sample of cells_from cord blood or peripheral_blood_into_a growth medium, causing cells_of the target cell population to_divide, and contacting_the cells in the growth medium with_a_selection element comprising binding molecules with_specific_affinity (such as a monoclonal antibody_for_CD34)_for a predetermined population of_cells (such as CD34 cells), so_as to select cells of the predetermined target population_from other cells_in the growth medium.
Rodgers et_al_(U.S._Pat. No. 6