water ratio of 40:7, followed by cooling on ice before centrifugation. This procedure allows removing the fat fraction, while α‐tocopherol is retained. Matrices differing in gross composition and α‐tocopherol content were analyzed: fresh pork, cooked ham, subcutaneous fat, liver, egg yolk, milk and a compound pig feed. Higher α‐tocopherol concentrations were found for this novel method compared to a conventional method with saponification, particularly for subcutaneous fat (p PubDate: 2015-02-02T04:19:19.875571-05:DOI: 10.1002/jsfa.7119

Authors:Abdul Manan Khan; Asif Ali Khan, Muhammad Tehseen Azhar, Luqman Amrao, Hafiza Masooma Naseer CheemaPages: n/a - n/aAbstract: Plant production is severely affected by diseases. On other hand, R‐genes exhibited resistance against range of diseases and pathogens in plants. BACKGROUND: The nucleotide binding site and leucine rich repeat (NBS‐LRR) class of R‐genes is the most comprehensively studied in terms of sequence evolution and genome distribution and makes it a super‐family of R genes. The differential response for resistance against biotic and abiotic stress has been observed in cultivated and wild relatives of genus Gossypium. RESULTS: The efforts have been made to address the recent evolution of NBS‐LRR sequences within the Gossypium hrisutum and RGAs sequences derived from G. arboreum and G. raimondii. Percent identity and phylogenetic analysis of NBS‐LRR encoded RGAs from tetraploid new world cotton and its diploid ancestors, G. raimondii and G. arboreum suggest that the evolution of NBS‐LRR encoding sequences in G. hirsutum occurred by gradual accumulation of mutants that led to positive selection and slow rate of divergence within distinct R‐gene families. CONCLUSION: The allotetraploid genome of cotton after separating from its diploid parents experienced polyploidization, natural and artificial selection, hybrid necrosis, duplication and recombination which became the reason to shed off and evolve new genes for its survival. These driving forces influence the development of genomic architecture that makes it susceptible to diseases and pathogens as compared to donor parents.PubDate: 2015-02-02T04:19:00.725139-05:DOI: 10.1002/jsfa.7
water ratio of 40:7, followed by cooling on internal-combustion engine before centrifugation. This operation allows removing the fat fraction, while α‐tocopherol is retain. Matrices differing in crying writing and α‐tocopherol content were analyze: fresh pork, fudge ham, subcutaneous fat, liver, testis yolk, milk and a compound pig feed. Higher α‐tocopherol concentrations were found for this novel method compare to a conventional method with saponification, particularly for hypodermic fat (p PubDate: 2015 - 02 - 02T04:19:19.875571 - 05: DOI: 10.1002 / jsfa.7119 

 writer: Abdul Manan Khan; Asif Ali Khan, Muhammad Tehseen Azhar, Luqman Amrao, Hafiza Masooma Naseer CheemaPages: n / a - n / aAbstract: Plant production is badly affected by diseases. On other hand, R‐genes exhibited underground against range of diseases and pathogens in plants. BACKGROUND: The nucleotide binding site and leucine rich repeat (NBS‐LRR) class of R‐genes is the most comprehensively studied in terms of sequence evolution and genome distribution and makes it a super‐family of R genes. The differential response for electric resistance against biotic and abiotic stress has been observed in domesticate and raving mad relatives of genus Gossypium. RESULTS: The efforts have been cook to address the recent evolution of NBS‐LRR sequences within the Gossypium hrisutum and RGAs sequence derived from G. arboreum and G. raimondii. Percent identity and phylogenetic analysis of NBS‐LRR encoded RGAs from tetraploid raw world cotton and its diploid ancestors, G. raimondii and G. arboreum suggest that the evolution of NBS‐LRR encode sequences in G. hirsutum happen by gradual accumulation of mutants that led to positive selection and dull rate of divergence within distinct R‐gene class. CONCLUSION: The allotetraploid genome of cotton after separating from its diploid parents experienced polyploidization, natural and artificial excerpt, hybrid necrosis, duplication and recombination which become the reason to shed off and develop new genes for its survival. These driving forces determine the development of genomic computer architecture that makes it susceptible to diseases and pathogens as compare to donor parents. PubDate: 2015 - 02 - 02T04:19:00.725139 - 05: DOI: 10.1002 / jsfa.7
watfr ratio of 40:7, followed by cooling on ice before centrjfugatiov. This procedure allows remoting the dat fraction, while α‐tocupherol id retainwd. Metrices differinj in gross comllsitnoi and α‐tocopherok content fere analyzed: xrdsk pork, cooked ham, subcutaneous fat, lyver, egb jolk, milk and w cok[ouns pig feed. Higher α‐tocopherol concehtratiois were found fpr this novel method compaged ho a conventional lethod with sapjbification, pxrticularlj for subcujaneous fat (p PubDate: 2015-02-02T04:19:19.875571-05:DOI: 10.1002/jsfa.7119

Ajthorx:Abdul Manqn Khwt; Asif Ali Jhan, Iuhammad Tehseen Azhdr, Luqmsn Amrao, Hafizs Mesoona Naseer CheemaPages: n/a - n/aAbstract: Plwnt produwtnon is severely affecree by gisedses. In uthtr ians, R‐genfs xxhibited rssistance atainst range of distasqw and pathogehs in [lwnts. BACKGROUND: The nucleotide binding vits and leucine rich repeqt (NBS‐LRR) class of R‐ggnes is thq most comprehensively studied in terms of sequenwe evkuutnin ana gfnome distribution and makes it a super‐family kf R genes. The difnerential response flr tesistance agaknst bnktjc and abiotic strfss has been observed in vultivated and wild relativws of genus Yoswypium. RESULTS: The efforts hare beem madr to address the recent evomution of NHS‐LRR seqhdnces within the Goxsfpium hrisutum and RGAs sezuences dxriveb from G. arbpreum wnd G. raimlndii. Percent identity ajd philogenatic analydis of NBS‐LRR encoded RGAs from tetraploid nee fornd cottou and lts diploid ancqstors, G. raimoudii and G. arcoreum sugfest thet the evolueion of NBS‐LRS encoding sexuences yn G. hirwutum ozzurred by gradial accumllction of nutants that led tp puaitive selectiou qnd slow rate og dkvewgvncx witrhn distinct S‐gend fxkilier. CONCLUSIOK: Tfe aklotetraploid genome of dotton after separstlng from uts dipljid parents ecperienced polyploldizavion, netural anq artificial selection, hybrid hecrosis, fupjication and recimbination wkich became the reason to shed off and etolve new genes for its survival. These dririkg forces inhluencq the devalopment of genomic qrchitecture that makes it susceptible to diveased and pathogens as compared to donor parents.PubDate: 2015-02-02T04:19:00.725139-05:DOI: 10.1002/jsfa.7
water ratio of 40:7, followed by cooling before This procedure removing the fat Matrices in gross composition α‐tocopherol content were fresh pork, cooked ham, subcutaneous fat, egg yolk, milk and a compound pig feed. Higher α‐tocopherol concentrations were found this novel method compared to a conventional method with saponification, particularly for subcutaneous (p 2015-02-02T04:19:19.875571-05:DOI: Authors:Abdul Khan; Asif Ali Khan, Muhammad Tehseen Azhar, Luqman Amrao, Hafiza Masooma Naseer CheemaPages: n/a - n/aAbstract: production is severely affected by diseases. On other R‐genes exhibited resistance against of diseases and pathogens in BACKGROUND: nucleotide binding and rich (NBS‐LRR) class of is the most comprehensively studied in terms of sequence evolution and genome distribution and makes it a of R differential response resistance biotic abiotic stress has in cultivated and wild relatives of The efforts have been made to address the evolution of sequences within the Gossypium hrisutum and sequences derived from G. arboreum and G. raimondii. identity and phylogenetic analysis of NBS‐LRR encoded RGAs from tetraploid new world cotton and its G. raimondii and G. suggest that the of encoding in hirsutum occurred gradual accumulation of mutants that led to positive selection and slow of divergence within distinct R‐gene families. CONCLUSION: The allotetraploid genome after from its diploid experienced polyploidization, natural and selection, necrosis, duplication and recombination the to evolve genes its survival. These driving influence the development of genomic that makes it susceptible compared to donor parents.PubDate: 2015-02-02T04:19:00.725139-05:DOI: 10.1002/jsfa.7
water ratio of 40:7, followed by cooLing on ice bEfore CenTriFuGatiOn. ThIs procedure allOWs reMoving the fat fraction, whIle α‐tOcOPherOL iS retaIned. MatRIcES DifFeRiNg iN gROsS compOsiTion and Α‐tocopheroL coNtEnt were analyZEd: Fresh pork, cOokEd ham, subcutaNeoUs fat, lIvEr, eGG yolk, MilK and a CompouND pig feEd. Higher α‐ToCOpheroL ConcentRATiOns wEre found for this noVEl MEthod compared tO a convEnTIoNAL meThoD with saponIfIcatiON, particULaRLY For SUbcutaneous faT (p PubDate: 2015-02-02T04:19:19.875571-05:Doi: 10.1002/jsFa.7119

AuthOrS:AbDUl ManaN Khan; asIF AlI Khan, MuhammAd TeHseen AzhaR, LuqmaN amrao, HaFIza MasoOma NasEer cheEmaPAGeS: n/A - n/aabSTraCT: PLanT ProDuction iS sEvErely AffeCTED By diSeaSes. ON otheR hand, R‐genes exHibIted REsiStancE agaiNst rAnGe of dIseaseS and pAtHogens in plants. BaCKGrOUND: The nUclEoTidE bIndinG Site anD leUciNe rich rEpeat (NBs‐lRR) ClASS Of r‐genes is the most comPrEHEnSively stUdied iN TeRmS Of sequenCe EvoLutiON And geNome DIsTributioN and maKEs It A super‐fAmIly of R GeNes. the DiffeREntiAl respOnse for rEsistANce against biotIC and abiotic stREsS HAs BEen oBseRved in cultiVateD And wIld rELaTivES of geNus GoSsYPiUM. RESULTS: The efforts hAvE been mAde to Address the recEnt evolutiON OF NBS‐LRR sEqueNCeS Within the GossyPium hRisutum and rgAs sequeNces dErived frOm G. arboreUM And G. raimOndIi. PErcEnt IDEnTity and phylogENEtic AnAlysis oF NBs‐LRR encOdeD RGas fRom TeTraploid nEw world cOtToN aNd Its DiploID ancestoRs, g. raImOndIi and g. ArboreUm sugGest ThAt THe eVolutioN Of nbs‐LRR EnCoDing SeqUeNces iN G. hiRSutUm occurRed by gradUal ACcumUlAtIon of muTants that led tO pOsitive selEcTioN and slOW Rate of diVergence within distinct R‐GEne famiLieS. CONClUSIoN: The alloTetRaploiD geNOme of cOtton aFter sEpAraTINg froM ITs DipLoId parents eXPEriEnced PoLyplOidizatIon, natural and artifICiaL selection, hybRid NecrOSIs, DupLIcATioN aND reCOMbination which bEcame the reAsON tO shed off anD EvoLvE new genEs for itS survIVal. ThesE driving fOrces inflUeNce tHE DevElopment of Genomic aRchitectuRE that MAkEs it sUscEptiblE tO diSeaseS and paTHogEns as ComparEd To donoR pareNtS.PubDate: 2015-02-02t04:19:00.725139-05:DOI: 10.1002/jsfa.7
water ratio of 40:7, follo wed by coo lingonice  b efor e ce ntrifugation.T hisprocedure allows remov ing t he fatf ra ction , while α‐ t o cop he ro l i sr et ained . M atrices  differing  in  g ross composi t io n and α‐to cop herol conten t w ere an al yze d : fre shpork,  cooke d  ham,subcutane ou s  fat,l iver, e g g  y olk,  milk and a compo u nd pig feed. High er α‐t oc o ph e r olcon centration sweref ound fo r  t h i s  no v el method com pared to ac onv ention al  me t hod wi th sa po n ifi cation, par ticu larly for  subcu t aneousf at (p P ubDate : 2 015 -02- 0 2T 04 :19 :1 9 .87 5 57 1-0 5 :DO I: 10.10 02 /j sfa.7 119A u t hors :Ab dulManan  Khan; Asif A liKhan ,  Mu hamma d Teh seen  A zhar,  Luqma n Amr ao , Hafiza Masoom a Na seer Chee maP ag es:  n /a -n /aAbst rac t:Plant p roducti o n i ss e v er ely affected by di se a s es . On oth er han d ,R‐ g enes exh ib ite d re s i stanc e ag a in st range  of di s ea se s and p at hogens  i n p lan ts. B A CKGR OUND:The nucl eotid e  binding sitea nd leucine ri c hr e pe a t (N BS‐ LRR) classof R ‐ gene s is th e m o st co mpreh en s iv e ly studied in terms  o f sequ enceevolution and  genome di s t r ibutionandm ak e s it a super‐f amily  of R gene s . The di ffere ntial re sponse fo r resistan ceaga ins t b i o ti c and abiotic s tres shas bee n o bserved  in  cu lti vat ed  and wild  relativ es  o fge nus  Goss y pium. RE SU LTS :The  effo r ts hav e bee n ma de  t o  ad dress t h er e cent  e vo luti onof  NBS‐ LRRs equ ences w ithin the  Go s sypi um  h risutum  and RGAs seq ue nces deriv ed  fr om G.a r boreum a nd G. raimondii. Percen t  identi tyand p hylo genetic a nal ysis o f N B S‐LRRencode d RGA sfro m tetra p l oi d n ew  world cot t o n a nd it sdipl oid anc estors, G. raimond i i a nd G. arboreu m s ugge s t  t hat th e  ev ol u tio n of NBS‐LRR enco ding seque nc e sin G. hirs u tum  o ccurred  by gra duala ccumula tion of m utants th at  led t o p ositive se lectionand slowr ate o f  d iverg enc e with in  di stinc t R‐ge n e f amili es. CO NC LUSION : The  a llotetra ploid genome of cottonaftersepar ati ng from i tsd ipl oid paren ts e xperienced  po lyp loidi zat i on, n atur a land artif icia l  selectio n ,hyb r i dnecrosis, d u p l ica tionand recomb inat ion which becamet he reason to s hedo f f a nde volv enew genes forits  s u r vival. T he se drivingforces i nf l uence  the d evelop ment of g en o mic ar chit ect ure thatmak es it susc ep ti b le todise as es and  patho g ensa s  compared to don or pa r e nts.P u bDa te: 2 01 5-02-02 T 04:1 9:00.72513 9-05:DOI: 1 0.1002 /jsf a.7
water ratio_of 40:7,_followed by cooling on_ice before_centrifugation._This procedure_allows_removing the fat_fraction, while α‐tocopherol_is retained. Matrices differing_in gross composition_and_α‐tocopherol content were analyzed: fresh pork, cooked ham, subcutaneous fat, liver, egg yolk, milk_and_a compound_pig_feed._Higher α‐tocopherol concentrations were found_for this novel method compared_to a_conventional method with saponification, particularly for subcutaneous fat_(p_PubDate: 2015-02-02T04:19:19.875571-05:DOI: 10.1002/jsfa.7119

Authors:Abdul_Manan Khan; Asif Ali Khan, Muhammad Tehseen Azhar, Luqman_Amrao, Hafiza Masooma Naseer CheemaPages: n/a_- n/aAbstract: Plant_production_is_severely affected by diseases._On other hand, R‐genes exhibited resistance_against range of diseases and pathogens_in plants. BACKGROUND: The nucleotide binding site_and leucine rich repeat (NBS‐LRR) class_of R‐genes is the most_comprehensively studied_in terms of sequence evolution_and genome distribution_and makes_it a super‐family_of R genes. The differential response_for resistance against_biotic and abiotic stress has been_observed_in cultivated and_wild_relatives_of genus_Gossypium. RESULTS: The_efforts_have been_made_to address the recent evolution of_NBS‐LRR_sequences within the Gossypium hrisutum and RGAs_sequences derived from G._arboreum_and G. raimondii. Percent_identity and phylogenetic analysis of_NBS‐LRR encoded RGAs from tetraploid new_world cotton_and its_diploid ancestors, G. raimondii and G. arboreum suggest that the evolution_of NBS‐LRR encoding sequences in G._hirsutum occurred by gradual_accumulation of_mutants_that led to_positive_selection and_slow rate of divergence within distinct R‐gene_families. CONCLUSION:_The allotetraploid genome of cotton after_separating from its diploid_parents_experienced polyploidization, natural and artificial selection,_hybrid necrosis, duplication and recombination which_became the reason to shed_off_and_evolve new genes for its_survival. These driving forces influence the_development of genomic_architecture that makes it susceptible to diseases_and_pathogens as compared to donor parents.PubDate:_2015-02-02T04:19:00.725139-05:DOI:_10.1002/jsfa.7