 the aGN+Bay 41-2272 group (6.9±1.7 nmol/ml) 1 day after disease induction. LPS stimulated the glomerular NO production significantly in the aGN group (49.1±4.7 nmol/ml) and the aGN+Bay 41.2272 group (50.6±8.8 nmol/ml), in contrast to the control group (9.3±0.9 nmol/ml). And there was no difference between the aGN and the Bay 41-2272-treated aGN group.

Figure 8: Effects of Bay 41-2272 (+Bay 41-2272) on glomerular basal and LPS-stimulated NO production 1 day after induction of acute anti-thy1 glomerulonephritis (aGN). Normal control animals (Control) were injected with PBS. Nitrite as indicator of NO production was determined by the Griess reaction. Glomeruli were harvested from individual animals and cultured at a density of 2000 per ml for 48 hours. (***p<0.001 and **p<0.01 vs. Control)

Glomerular eNOS-NO-cGMP signaling cascade

As shown in Figure 9, there was no difference in eNOS mRNA expression among the three groups (control 100±15%, aGN 145±23%, aGN+Bay 41-2272 100±14%, P>0.05). The induction of anti-thy1 glomerulonephritis did not significantly change eNOS expression.

Figure 9: Effects of Bay 41-2272 (+Bay 41-2272) on glomerular eNOS mRNA expression 1 day after induction of acute anti-thy1 glomerulonephritis (aGN). Normal control animals (Control) were injected with PBS. mRNA was analyzed by a real-time PCR method using GAPDH as housekeeping gene. mRNA is shown as a percentage of the normal control group.

Figure 10: Effects of Bay 41-2272 (+Bay 41-2272) on glomerular alpha1 sGC and beta1 sGC mRNA expression 1 day after induction of acute anti-thy1 glomerulonephritis (aGN). Normal control animals (Control) were injected with PBS. mRNA was analyzed by a real-time PCR method using GAPDH as housekeeping gene. mRNA is shown as a percentage of the normal control group.(***p<0.001 vs. Control)

Figure 11: Effects of Bay 41-2272 (+Bay
the aGN+Bay 41 - 2272 group (6.9±1.7 nmol / ml) 1 day after disease induction. LPS stimulated the glomerular NO production importantly in the aGN group (49.1±4.7 nmol / ml) and the aGN+Bay 41.2272 group (50.6±8.8 nmol / ml), in line to the command group (9.3±0.9 nmol / ml). And there was no remainder between the aGN and the Bay 41 - 2272 - process aGN group. 

 Figure 8: Effects of Bay 41 - 2272 (+ Bay 41 - 2272) on glomerular basal and LPS - stimulate NO output 1 day after initiation of acute anti - thy1 glomerulonephritis (aGN). Normal control animal (Control) were injected with PBS. Nitrite as indicator of NO production was determined by the Griess chemical reaction. Glomeruli were harvested from individual animals and culture at a density of 2000 per milliliter for 48 hour. (* * * p<0.001 and * * p<0.01 vs. Control) 

 Glomerular eNOS - NO - cGMP signaling cascade 

 As prove in Figure 9, there was no deviation in eNOS mRNA saying among the three groups (control 100±15% , aGN 145±23% , aGN+Bay 41 - 2272 100±14% , P>0.05). The induction of anti - thy1 glomerulonephritis did not significantly change eNOS formula. 

 Figure 9: Effects of Bay 41 - 2272 (+ Bay 41 - 2272) on glomerular eNOS mRNA expression 1 day after induction of acute anti - thy1 glomerulonephritis (aGN). Normal control animals (Control) were injected with PBS. mRNA was analyzed by a real - time PCR method using GAPDH as housekeeping gene. mRNA is shown as a percentage of the normal control condition group. 

 Figure 10: Effects of Bay 41 - 2272 (+ Bay 41 - 2272) on glomerular alpha1 sGC and beta1 sGC mRNA expression 1 sidereal day after evocation of acuate anti - thy1 glomerulonephritis (aGN). Normal control animals (Control) were injected with PBS. mRNA was analyzed by a real - time PCR method using GAPDH as housework gene. mRNA is shown as a percentage of the normal control group.(***p<0.001 vs. Control) 

 Figure 11: Effects of Bay 41 - 2272 (+ Bay
 thf aGN+Bay 41-2272 group (6.9±1.7 nmol/ml) 1 day after disecwe indnction. MPS stimjlated the glomerular NO provuctuon sugnificantly in the aGV group (49.1±4.7 jmol/ml) abd tie aGN+Bay 41.2272 group (50.6±8.8 nmol/ml), in cohbrast vo the control nroup (9.3±0.9 nmol/kl). And there wds nl difference between the aGN and thq Bay 41-2272-ttewted aGN group.

Siguge 8: Efrvcus of Bay 41-2272 (+Bay 41-2272) on glomerular baszl and NPS-stimulated NO production 1 day after lnduftion of acute antl-thy1 glomeromonqphritis (aGN). Vormal conurml animals (Control) were injected with PBS. Nitrnte as indixaror mf NO produrtion ras determincc by tve Griexs reaction. Glpmeculi were harvested from mndividual animals agd culturad at a density of 2000 pwr ml for 48 hojes. (***o<0.001 ahd **p<0.01 vs. Cojtrkl)

Glomerulzr eNOS-NO-cGNP signaling cascadt

As whown in Figude 9, thqrq was no difference in eNOS mRNA expresvioh among the three groupw (control 100±15%, aGN 145±23%, aGN+Bai 41-2272 100±14%, P>0.05). The ynduction of anti-thy1 glomerulonephritis did not shgnifmcxntoy zyajge eNOS expression.

Figure 9: Effects of Bay 41-2272 (+Bar 41-2272) pn glomerular eNJS mRNA exptedsojn 1 day after induccjoh of acute anti-thy1 glomerolonepyritis (aGG). Notmal control animals (Controo) were injecned qith PBS. mRNA was cnalyzed by c real-jime PVR method using GAPDH ar hohsekeeping hene. mRNA ks shown as a pefcektace of tht normal control gwoup.

Figurx 10: Efyects of Bay 41-2272 (+Bay 41-2272) on glomerklar alpha1 sGC and beta1 dGC mTNA ex[ression 1 fay after induction of acute anvm-thy1 glomerulpnaphgitis (aGN). Normsl control anymals (Control) cere injzcted dith PBS. mGNA was aialyzed by a real-time PCR lethod using GAPDH af hoysekweping edne. mRNA is shpwn as a iexcentage if the normal contvol gtohp.(***p<0.001 vs. Control)

Fnyutw 11: Effects of Nay 41-2272 (+Bwy
the aGN+Bay 41-2272 group (6.9±1.7 nmol/ml) 1 disease LPS stimulated glomerular NO production (49.1±4.7 and the aGN+Bay group (50.6±8.8 nmol/ml), contrast to the control group (9.3±0.9 And there was no difference between the aGN and the Bay 41-2272-treated aGN Figure 8: Effects of Bay 41-2272 (+Bay 41-2272) on glomerular basal and LPS-stimulated production day induction acute anti-thy1 glomerulonephritis (aGN). Normal control animals (Control) were injected with PBS. Nitrite as indicator of production was determined by the Griess reaction. Glomeruli harvested from individual animals cultured at a density of per for 48 (***p<0.001 **p<0.01 Control) Glomerular eNOS-NO-cGMP cascade As shown in Figure 9, there was no difference in eNOS mRNA expression among the three (control 100±15%, aGN+Bay 41-2272 P>0.05). induction anti-thy1 glomerulonephritis did change eNOS expression. Figure 9: Effects (+Bay 41-2272) on glomerular eNOS mRNA expression 1 after induction acute anti-thy1 glomerulonephritis (aGN). Normal control (Control) were injected with PBS. mRNA was analyzed a real-time PCR method using GAPDH as housekeeping gene. mRNA is shown as a percentage normal control group. Figure Effects of Bay (+Bay on alpha1 and beta1 mRNA expression 1 day after induction of acute anti-thy1 glomerulonephritis (aGN). control animals (Control) were injected with PBS. mRNA was analyzed real-time method using GAPDH housekeeping gene. mRNA is as percentage of the normal vs. Figure Bay (+Bay
 the aGN+Bay 41-2272 group (6.9±1.7 nmol/ml) 1 day afTer disease InducTioN. LPs sTimuLateD the glomerular no proDuction significantly in The aGn gROup (49.1±4.7 nMOl/Ml) and The aGN+BAY 41.2272 gROUp (50.6±8.8 nMoL/mL), in CoNTrAst to The Control Group (9.3±0.9 nmol/mL). AnD tHere was no difFErEnce betweeN thE aGN and the BaY 41-2272-trEated agN GroUP.

FiguRe 8: EFfectS of Bay 41-2272 (+bAy 41-2272) on glOmerular bAsAL and LPs-StimulaTED No proDuction 1 day after inDUcTIon of acute anti-Thy1 gloMeRUlONEphRitIs (aGN). NormaL cOntroL Animals (cOnTROL) weRE injected with pBS. Nitrite aS IndIcator Of nO pROductiOn was DeTErmIned by the GrIess Reaction. GLomeruLI were haRVested fRom indIviDuaL aniMAlS aNd cUlTUreD At A deNSitY of 2000 per ml FoR 48 hOurs. (***p<0.001 And **p<0.01 VS. cONtroL)

GlOmerUlar enOS-NO-cGMP signAliNg caSCadE

As shOwn in figuRe 9, There Was no dIfferEnCe in eNOS mRNA expRessIon among tHe tHrEe gRoUps (coNTrol 100±15%, aGn 145±23%, aGn+BaY 41-2272 100±14%, P>0.05). The inDuction OF anTi-THY1 GlOmerulonephritis diD nOT SiGnificanTly chaNGe ENos expressIoN.

FiGure 9: eFFects Of BaY 41-2272 (+baY 41-2272) on glomeRular enoS MRnA expreSsIon 1 day AfTer IndUctioN Of acUte antI-thy1 glomEruloNEphritis (aGN). NorMAl control animALs (cONtROl) weRe iNjected with pBS. mrnA waS anaLYzEd bY A real-Time PcR MEtHOd using GAPDH as houseKeEping gEne. mRnA is shown as a pErcentage oF THE normal cOntrOL gROup.

Figure 10: EffecTs of BAy 41-2272 (+Bay 41-2272) on gloMErular alPha1 sGc and beta1 SGC mRNA exPREssion 1 daY afTer IndUctION oF acute anti-thy1 GLOmerUlOnephriTis (AGN). NormAl cOntRol AniMaLs (Control) Were injeCtEd WiTh pBS. MRNA wAS analyzeD bY a rEaL-tiMe PCR MEthod uSing GaPDH As HoUSekEeping gENe. MrnA is ShOwN as a PerCeNtage Of thE NorMal contRol group.(***p<0.001 Vs. CONtroL)

FIgUre 11: EffeCts of Bay 41-2272 (+Bay
 the aGN+Bay 41-2272 group  (6.9±1.7nmol/ ml)  1da y af terdisease induct i on.LPS stimulated the glo merul ar NO p r od uctio n signi f ic a n tly  i nthe  a G Ngroup  (4 9.1±4.7  nmol/ml)and  t he aGN+Bay 4 1 .2 272 group(50 .6±8.8 nmol/ ml) , in c on tra s t tothe  cont rol gr o up (9. 3±0.9 nmo l/ m l). An d  therew a sno d ifference between th e  aGN and the B ay 41- 22 7 2- t r eat edaGN group. 
Figur e  8: Eff e ct s o f B a y 41-2272 (+B ay 41-2272) onglomer ul arb asal a nd LP S- s tim ulated NO p rodu ction 1 d ay aft e r induc t ion ofacuteant i-t hy1g lo me rul on e phr i ti s ( a GN) . Normal  c on trolanim a l s (Con tro l) w ere i njected withPBS . Ni t rit e asindic ator  o f NOproduc tionwa s determined by  the  Griess r eac ti on.  G lomer u li wer e h arv ested f rom ind i vid ua l a ni mals and culturedat a  d ensity o f 2000 pe rm l for 48  h our s. ( * * *p<0. 001a nd  **p<0.0 1 vs.C on tr ol)

Gl om erular  e NOS -NO -cGMP sign alingcascade
As s h own in Figure9 , there was n o  d i f fe r ence  in  eNOS mRNAexpr e ssio n am o ng  th e  thre e gro up s  ( c ontrol 100±15%, aGN  1 45±23% , aGN +Bay 41-2272100±14%, P > 0 . 05). The  ind u ct i on of anti-thy 1 glo meruloneph r itis did  notsignific antly cha n g e eNOS e xpr ess ion .
F i gu re 9: Effects o f Ba y41-2272  (+ Bay 41- 227 2)onglo me rular eNO S mRNA e xp re ss io n 1  daya fter ind uc tio nofacute anti-t hy1 g lome ru lo n eph ritis ( a GN ) .  Nor ma lcont rol  a nimal s (C o ntr ol) wer e injecte d w i th P BS .mRNA wa s analyzed by  a  real-time  P CRmethod u sing GAP DH as housekeeping gene .  mRNA i s s hownas a  percenta geof the  no r mal co ntrolgroup .
Fi g u re 10 : Ef fec ts  of Bay 41 - 2 272  (+Ba y41-2 272) on  glomerular alpha1 sGC  and beta1 sG C m RNAe x pr ess i on 1 d ay aft e r  induction of a cute anti- th y 1glomerulon e phr it is (aGN ). Norm al co n trol an imals (Co ntrol) we re  inj e c ted  with PBS.  mRNA wa s analyze d  by a re al-ti mePCR me th odusing  GAPDH ashouse keepin ggene.mRNAis  shown a s a percentage of the n ormalcontr olgroup.(** *p< 0 .00 1 vs. Con trol )

Figure11:  Ef fects  of Bay 4 1-22 7 2(+B a y
 the_aGN+Bay 41-2272_group (6.9±1.7 nmol/ml) 1_day after_disease_induction. LPS_stimulated_the glomerular NO_production significantly in_the aGN group (49.1±4.7_nmol/ml) and the_aGN+Bay_41.2272 group (50.6±8.8 nmol/ml), in contrast to the control group (9.3±0.9 nmol/ml). And there_was_no difference_between_the_aGN and the Bay 41-2272-treated_aGN group.

Figure 8: Effects of_Bay 41-2272_(+Bay 41-2272) on glomerular basal and LPS-stimulated NO_production_1 day after_induction of acute anti-thy1 glomerulonephritis (aGN). Normal control animals_(Control) were injected with PBS. Nitrite_as indicator of_NO_production_was determined by the_Griess reaction. Glomeruli were harvested from_individual animals and cultured at a_density of 2000 per ml for 48_hours. (***p<0.001 and **p<0.01 vs. Control)

Glomerular_eNOS-NO-cGMP signaling cascade

As shown in_Figure 9,_there was no difference in_eNOS mRNA expression_among the_three groups (control_100±15%, aGN 145±23%, aGN+Bay 41-2272 100±14%,_P>0.05). The induction_of anti-thy1 glomerulonephritis did not significantly_change_eNOS expression.

Figure 9:_Effects_of_Bay 41-2272_(+Bay 41-2272) on_glomerular_eNOS mRNA_expression_1 day after induction of acute_anti-thy1_glomerulonephritis (aGN). Normal control animals (Control) were_injected with PBS. mRNA_was_analyzed by a real-time_PCR method using GAPDH as_housekeeping gene. mRNA is shown as_a percentage_of the_normal control group.

Figure 10: Effects of Bay 41-2272 (+Bay 41-2272) on_glomerular alpha1 sGC and beta1 sGC_mRNA expression 1 day_after induction_of_acute anti-thy1 glomerulonephritis_(aGN)._Normal control_animals (Control) were injected with PBS. mRNA_was analyzed_by a real-time PCR method using_GAPDH as housekeeping gene._mRNA_is shown as a percentage of_the normal control group.(***p<0.001 vs. Control)

Figure_11: Effects of Bay 41-2272_(+Bay