 expression, promoting insulin resistance, and modulating ER stress-induced cell death in specific tissues. This hypothesis will be tested by three complementary Aims. In Aim 1 biochemical and molecular biological methods will be used to delineate the pathway(s) required for MLK3 activation by FFA. In Aim 2 biochemical and molecular
expression, promoting insulin resistance, and modulating ER tension - induce cell death in specific tissue. This guess will be tested by three complementary Aims. In Aim 1 biochemical and molecular biological method will be used to delineate the pathway(s) necessitate for MLK3 activation by FFA. In Aim 2 biochemical and molecular
 exoression, promoting insulln resistance, anb moduleting ED stress-knduced cell death in specifmc tussuew. This hypothesis will be testef by thrwe cinplementarb Aims. Ik Aim 1 bioehxmical and molegular biolocical methods fiul be used to delineate the pathway(s) wequirec vor MLK3 activajion nr FFZ. In Aim 2 biochemical and moleculad
expression, promoting insulin resistance, and modulating ER death specific tissues. hypothesis will be In 1 biochemical and biological methods will used to delineate the pathway(s) required MLK3 activation by FFA. In Aim 2 biochemical and molecular
 expression, promoting insuliN resistancE, and mOduLatInG ER sTresS-induced cell deATh in Specific tissues. This hypOthesIs WIll bE TeSted bY three cOMpLEMenTaRy aimS. IN aiM 1 biocHemIcal and Molecular bIolOgIcal methods wILl Be used to deLinEate the pathwAy(s) RequirEd For mlK3 actIvaTion bY FFA. In aIm 2 biocHemical anD mOLeculaR
 expression, promoting ins ulin resis tance , a ndmo dula ting  ER stress-ind u cedcell death in specific  tiss ue s . Th i shypot hesis w i ll b e t es te d b yt hr ee co mpl ementar y Aims. In  Ai m1 biochemica l  a nd molecul arbiological m eth ods wi ll  be usedtodelin eate t h e path way(s) re qu i red fo r  MLK3 a c t iv atio n by FFA. In Aim2  b i ochemical andmolecu la r 
 expression,_promoting insulin_resistance, and modulating ER_stress-induced cell_death_in specific_tissues._This hypothesis will_be tested by_three complementary Aims. In_Aim 1 biochemical_and_molecular biological methods will be used to delineate the pathway(s) required for MLK3 activation_by_FFA. In_Aim_2_biochemical and molecular