 motor function will be modified and functionally expressed. The motor function will then be analyzed by enzymatic analysis, biophysical analysis and in vitro motility assay,,with a particular emphasis on the single molecule assay system. The itemized specific aims are: 1. To determine the regulatory mechanisms of myosin VI motor function; 2) To
motor function will be modified and functionally expressed. The motor function will then be analyze by enzymatic psychoanalysis, biophysical analysis and in vitro motility assay,,with a especial stress on the single molecule assay organization. The itemized specific bearing are: 1. To determine the regulative mechanism of myosin VI motor function; 2) To
 mohor function will be modlfied and functionally xxpresssd. The mutor function will then be aialyzed bt enzymatic analysis, bkophysicap analysus aid in vitro motility assay,,with w paxtmcular emphasis on the sitgle molecule dsrav system. The itemized specific aims wre: 1. To dftermine the rggulauorr medhanisms of myosin VI motor functikn; 2) To
motor function will be modified and functionally motor will then analyzed by enzymatic vitro assay,,with a particular on the single assay system. The itemized specific aims 1. To determine the regulatory mechanisms of myosin VI motor function; 2) To
 motor function will be modifiEd and functIonalLy eXprEsSed. THe moTor function wilL Then Be analyzed by enzymatic aNalysIs, BIophYSiCal anAlysis aND iN VItrO mOtIliTy ASsAy,,witH a pArticulAr emphasis On tHe Single molecuLE aSsay system. the Itemized specIfiC aims aRe: 1. to dETermiNe tHe regUlatorY MechanIsms of myoSiN vI motoR FunctioN; 2) tO
 motor function will be mo dified and  func tio nal ly  exp ress ed. The motorf unct ion will then be analy zed b ye nzym a ti c ana lysis,b io p h ysi ca lana ly s is  andinvitro m otility as say ,, with a parti c ul ar emphasi s o n the single  mo lecule  a ssa y  syst em.  Theitemiz e d spec ific aims  a r e: 1.T o deter m i ne  the  regulatory mecha n is m s of myosin VI  motor  f u nc t i on;  2)  To
 motor_function will_be modified and functionally_expressed. The_motor_function will_then_be analyzed by_enzymatic analysis, biophysical_analysis and in vitro_motility assay,,with a_particular_emphasis on the single molecule assay system. The itemized specific aims are: 1. To_determine_the regulatory_mechanisms_of_myosin VI motor function; 2)_To