 we will systematically investigate this hypothesis via three specific aims. In Aim 1, we will perform experiments to establish the long-term stability of?-crystallin-client protein complexes under the conditions of the lens by employing fluorescence resonance energy transfer (FRET)- based assays. We will then determine whether?-crystallin-
we will systematically investigate this hypothesis via three specific aims. In Aim 1, we will perform experiments to install the retentive - term stability of?-crystallin - client protein complex under the conditions of the lens by employing fluorescence rapport department of energy transfer (FRET)- based assays. We will then determine whether?-crystallin-
 we will systematically invtstigate this hypothesis via tgree spezific aims. In Aim 1, we will pxrfoem exkvriments to establish the long-nerm stabulitb of?-crystallin-clmsnt probzin cklplerew under the cokditions of the lens by ekpuoving fluorescence resonance energy twansfer (FGET)- based assais. We rill nhtn determine whether?-crystallin-
we will systematically investigate this hypothesis via aims. Aim 1, will perform experiments of?-crystallin-client complexes under the of the lens employing fluorescence resonance energy transfer (FRET)- assays. We will then determine whether?-crystallin-
 we will systematically invesTigate this HypotHesIs vIa ThreE speCific aims. In Aim 1, WE wilL perform experiments to eStablIsH The lONg-Term sTabilitY Of?-CRYstAlLiN-clIeNT pRoteiN coMplexes Under the coNdiTiOns of the lens BY eMploying flUorEscence resonAncE energY tRanSFer (FReT)- bAsed aSsays. WE Will thEn determiNe WHether?-CRystallIN-
 we will systematically in vestigatethishyp oth es is v ia t hree specifica ims.  In Aim 1, we will per formex p erim e nt s toestabli s ht h e l on g- ter ms ta bilit y o f?-crys tallin-cli ent  p rotein compl e xe s under th e c onditions of  th e lens  b y e m ployi ngfluor escenc e  reson ance ener gy transf e r (FRET ) -  b ased  assays. We willt he n  determine whe ther?- cr y st a l lin -
 we_will systematically_investigate this hypothesis via_three specific_aims._In Aim_1,_we will perform_experiments to establish_the long-term stability of?-crystallin-client_protein complexes under_the_conditions of the lens by employing fluorescence resonance energy transfer (FRET)- based assays. We_will_then determine_whether?-crystallin-