 epithelial conversion of renal progenitor cells during renal development. We plan to clone Midkine receptor gene using either an expression cloning approach or ligand affinity chromatography to purify the MK receptor protein. Additionally, we also plan to examine Midkine signal transduction pathway, then define the basic developmental pattern in the kidney of expression of all
epithelial conversion of renal progenitor cells during renal development. We design to clone Midkine sense organ gene practice either an expression cloning approach or ligand affinity chromatography to purify the MK sense organ protein. Additionally, we also plan to examine Midkine signal transduction nerve pathway, then specify the basic developmental pattern in the kidney of saying of all
 eplthelial conversion of rtnal progenitor cglos durmng renzl develupment. We plan to clone Midkmne eecepujr gene using eithdr an expgession cooniig approach or lmfand afnnnity ghromctigraphy to purlfy the MK seceptor protehn. Abditionally, we also plan to examine Iidkine slgnal transducjion katrway, nhtn define the basic developmental patteri in the kidney of expression of all
epithelial conversion of renal progenitor cells during We to clone receptor gene using or affinity chromatography to the MK receptor Additionally, we also plan to examine signal transduction pathway, then define the basic developmental pattern in the kidney of of all
 epithelial conversion of renAl progenitOr celLs dUriNg RenaL devElopment. We plan TO cloNe Midkine receptor gene uSing eItHEr an EXpRessiOn cloniNG aPPRoaCh Or LigAnD AfFinitY chRomatogRaphy to purIfy ThE MK receptor pROtEin. AdditioNalLy, we also plan To eXamine miDkiNE signAl tRansdUction PAthway, Then definE tHE basic DEvelopmENTaL patTern in the kidney of EXpREssion of all
 epithelial conversion ofrenal prog enito r c ell sduri ng r enal developme n t. W e plan to clone Midkin e rec ep t or g e ne  usin g eithe r  a n exp re ss ion  c l on ing a ppr oach or  ligand af fin it y chromatogr a ph y to purif y t he MK recept orprotei n.  Ad d ition all y, we  alsop lan to  examineMi d kine s i gnal tr a n sd ucti on pathway, thend ef i ne the basic d evelop me n ta l pat ter n in the k id ney o f  expres s io n o f a l l
 epithelial_conversion of_renal progenitor cells during_renal development._We_plan to_clone_Midkine receptor gene_using either an_expression cloning approach or_ligand affinity chromatography_to_purify the MK receptor protein. Additionally, we also plan to examine Midkine signal transduction_pathway,_then define_the_basic_developmental pattern in the kidney_of expression of all