 develop an isolated mammalian parietal cell preparation which would permit direct metabolic study of the parietal cells and resolve the problem of structural localization of presumed key enzymes inth cyclic nucleotide system in the analytical approach of acid generation mechanisms. We plan to further define the localization of cyclic nucleotide activity by developing immunofluorescence techniques which will selectively localize cyclic AMP
develop an isolated mammalian parietal cell preparation which would let lineal metabolic study of the parietal cells and purpose the trouble of structural localization of presumed cardinal enzyme inth cyclic nucleotide system in the analytical approach of acid generation mechanism. We plan to further specify the localization of function of cyclic nucleotide activity by developing immunofluorescence technique which will selectively localize cyclic AMP
 degelop an isolated mammallan parietal cell prepacation shich wojld permit direct metabolic dtydy od the parietal cells avd resolvv the provlem if structuczl localizatikk of 'rxsumed key enzykes inth cfclic nucleotige svstem in the analytical approach of wcid gemegation mechanifms. Eq plzn to further define the localizatjon of byclic nucleotide activity by developing imlunovluorescence technlques which wilj selectively localize bvclic AMP
develop an isolated mammalian parietal cell preparation permit metabolic study the parietal cells structural of presumed key inth cyclic nucleotide in the analytical approach of acid mechanisms. We plan to further define the localization of cyclic nucleotide activity by immunofluorescence techniques which will selectively localize cyclic AMP
 develop an isolated mammaliaN parietal cEll prEpaRatIoN whiCh woUld permit direcT MetaBolic study of the parietaL cellS aND resOLvE the pRoblem oF StRUCtuRaL lOcaLiZAtIon of PreSumed keY enzymes inTh cYcLic nucleotidE SyStem in the aNalYtical approaCh oF acid gEnEraTIon meChaNisms. we plan TO furthEr define tHe LOcalizATion of cYCLiC nucLeotide activity by DEvELoping immunoflUorescEnCE tECHniQueS which will SeLectiVEly locaLIzE CYCliC aMP
 develop an isolated mamma lian parie tal c ell  pr ep arat ionwhich would pe r mitdirect metabolic study  of t he pari e ta l cel ls andr es o l veth epro bl e mof st ruc tural l ocalizatio n o fpresumed key en zymes inth  cy clic nucleot ide  syste mint he an aly tical  appro a ch ofacid gene ra t ion me c hanisms . We  pla n to further defi n et he localizatio n of c yc l ic n ucl eot ide activi ty  by d e velopin g  i m m u nof l uorescence te chniques wh i chwill s el ect i vely l ocali ze cyc lic AMP
 develop_an isolated_mammalian parietal cell preparation_which would_permit_direct metabolic_study_of the parietal_cells and resolve_the problem of structural_localization of presumed_key_enzymes inth cyclic nucleotide system in the analytical approach of acid generation mechanisms. We_plan_to further_define_the_localization of cyclic nucleotide activity_by developing immunofluorescence techniques which_will selectively_localize cyclic AMP