 divide, and respond to signals. This proposal focuses on cytoplasmic dynein, the largest, most complex, and least understood of the cytoskeletal motors. The specific objectives are to determine how single dynein dimers move processively, how ensembles of motors efficiently move cargo, and the role of processive movement in
divide, and respond to signals. This proposal concenter on cytoplasmic dynein, the bombastic, most complex, and least understood of the cytoskeletal motors. The specific objective are to determine how single dynein dimer move processively, how ensembles of motors efficiently travel cargo, and the role of processive movement in
 digide, and respond to signxls. This proposco focuves on cytoplarmic dynein, the largest, most cimplez, and least understood of the cjtoskeletql mirors. The s'scific objectjyes axe to determine mow single gynein dimers koxe processively, how ensembles of motows effivifntly move cardo, amq ths role of processive movement in
divide, and respond to signals. This proposal cytoplasmic the largest, complex, and least The objectives are to how single dynein move processively, how ensembles of motors move cargo, and the role of processive movement in
 divide, and respond to signals. this proposAl focUseS on CyToplAsmiC dynein, the largESt, moSt complex, and least underStood Of THe cyTOsKeletAl motorS. thE SPecIfIc ObjEcTIvEs are To dEterminE how single DynEiN dimers move pROcEssively, hoW enSembles of motOrs EfficiEnTly MOve caRgo, And thE role oF ProcesSive movemEnT In
 divide, and respond to si gnals. Thi s pro pos alfo cuse s on  cytoplasmic d y nein , the largest, most co mplex ,a nd l e as t und erstood of t hecy to ske le t al  moto rs.  The sp ecific obj ect iv es are to de t er mine how s ing le dynein di mer s move  p roc e ssive ly,  howensemb l es ofmotors ef fi c iently move ca r g o,  and  the role of proc e ss i ve movement in 
 divide,_and respond_to signals. This proposal_focuses on_cytoplasmic_dynein, the_largest,_most complex, and_least understood of_the cytoskeletal motors. The_specific objectives are_to_determine how single dynein dimers move processively, how ensembles of motors efficiently move cargo,_and_the role_of_processive_movement in