 acid biosynthesis, transport, and regulation will be determined. The direct effects of these specific gene deletions on intestinal bile acid transport will be determined in vivo by measuring changes in bile acid fecal excretion and pool size, and in vitro using ileal everted gut sacs. The studies in Specific Aim 2 will directly test the hypothesis
acid biosynthesis, transport, and regulation will be determined. The direct effect of these specific gene deletion on intestinal bile acid transport will be determined in vivo by measuring change in bile acid fecal excretion and consortium size, and in vitro use ileal everted gut pouch. The studies in Specific Aim 2 will directly quiz the hypothesis
 acld biosynthesis, transporu, and regulation cull be deterjined. Thd direct effects of these spxcifuc gebe deletions on intestknal bile acid trqnspiet will be determikzd in yivo yy measuring chakges in bila acid fecal efcfecion and pool size, and in vitro usind ileal egerted gut sacf. Tht seudiss in Specific Aim 2 will directly fest tht hypothesis
acid biosynthesis, transport, and regulation will be direct of these gene deletions on be in vivo by changes in bile fecal excretion and pool size, and vitro using ileal everted gut sacs. The studies in Specific Aim 2 will test the hypothesis
 acid biosynthesis, transport, And regulatIon wiLl bE deTeRminEd. ThE direct effects OF theSe specific gene deletionS on inTeSTinaL BiLe aciD transpORt WILl bE dEtErmInED iN vivo By mEasurinG changes in BilE aCid fecal excrETiOn and pool sIze, And in vitro usIng Ileal eVeRteD Gut saCs. THe stuDies in sPecifiC Aim 2 will dIrECtly teST the hypOTHeSis
 acid biosynthesis, transp ort, and r egula tio n w il l be  det ermined. The d i rect  effects of these spec ificge n e de l et ionson inte s ti n a l b il eaci dt ra nspor t w ill bedetermined  in  v ivo by measu r in g changesinbile acid fe cal  excre ti ona nd po olsize,  and i n  vitro  using il ea l  evert e d gut s a c s.  The  studies in Speci f ic Aim 2 will dir ectlyte s tt h e h ypo thesis
 acid_biosynthesis, transport,_and regulation will be_determined. The_direct_effects of_these_specific gene deletions_on intestinal bile_acid transport will be_determined in vivo_by_measuring changes in bile acid fecal excretion and pool size, and in vitro using_ileal_everted gut_sacs._The_studies in Specific Aim 2_will directly test the hypothesis