-scanning methods, as the entire imaging plane is detected simultaneously. Second, excitation is confined to the focal plane, so each pixel is imaged only once during each volumetric acquisition. This drastically reduces light exposure and results in far lower photobleaching and photodamage than is possible with conventional imaging techniques.
-scanning methods, as the entire imaging plane is detect simultaneously. Second, excitement is confine to the focal plane, so each pixel is visualize only once during each volumetric acquisition. This drastically reduce light exposure and results in far lower photobleaching and photodamage than is possible with ceremonious imaging techniques.
-scajning methods, as the entlre imaging plang us detxcted sjmultaneuusly. Second, excitation is clndined to the focal plane, so each pixvl is imated ibly once dnding eagk volhletrnc acquisition. Tmis drasticdlly reduces lhgft exposure and results in far lower [hotoblrafhing and photjdamsde tgan is possible with conventional jmaging techniques.
-scanning methods, as the entire imaging plane simultaneously. excitation is to the focal imaged once during each acquisition. This drastically light exposure and results in far photobleaching and photodamage than is possible with conventional imaging techniques.
-scanning methods, as the entirE imaging plAne is DetEctEd SimuLtanEously. Second, exCItatIon is confined to the focaL planE, sO Each PIxEl is iMaged onLY oNCE duRiNg EacH vOLuMetriC acQuisitiOn. This drasTicAlLy reduces ligHT eXposure and ResUlts in far lowEr pHotoblEaChiNG and pHotOdamaGe than IS possiBle with coNvENtionaL Imaging TEChNiquEs.
-scanning methods, as theentire ima gingpla neis  det ecte d simultaneous l y. S econd, excitation is c onfin ed to t h efocal  plane, so e ach  p ix elis im agedonl y onceduring eac h v ol umetric acqu i si tion. This  dr astically re duc es lig ht  ex p osure  an d res ults i n  far l ower phot ob l eachin g  and ph o t od amag e than is possibl e  w i th conventiona l imag in g  t e c hni que s.
-scanning methods,_as the_entire imaging plane is_detected simultaneously._Second,_excitation is_confined_to the focal_plane, so each_pixel is imaged only_once during each_volumetric_acquisition. This drastically reduces light exposure and results in far lower photobleaching and photodamage_than_is possible_with_conventional_imaging techniques.