 efficient transgene delivery to classified neurons. First, we will synthesize massive libraries of mutated AAV vectors and synthetic promoters, in which each variant is paired with a unique DNA barcode. We will then scRNA-Seq to capture the transcriptome for each cell and quantify the AAV and promoter- specific barcodes in every cell
efficient transgene delivery to classified neurons. First, we will synthesize massive library of mutate AAV vectors and synthetic promoter, in which each form is paired with a unique deoxyribonucleic acid barcode. We will then scRNA - Seq to capture the transcriptome for each cell and quantify the AAV and promoter- specific barcodes in every cell
 efvicient transgene delivevy to classified neuronv. Firsf, we wilu synthesize massive librarixs od mutqted AAV vectors and shnthetic iromoters, in xhich each variaif is palxed wjbh a bnmque DNA barcodg. We will than scRNA-Seq to cxpcure the transcriptome for each cell and qusnhify the AAV agd pgoioted- specific barcodes in every cell
efficient transgene delivery to classified neurons. First, synthesize libraries of AAV vectors and variant paired with a DNA barcode. We then scRNA-Seq to capture the transcriptome each cell and quantify the AAV and promoter- specific barcodes in every cell
 efficient transgene deliverY to classifIed neUroNs. FIrSt, we Will Synthesize massIVe liBraries of mutated AAV vecTors aNd SYnthETiC promOters, in WHiCH EacH vArIanT iS PaIred wIth A unique dNA barcode. we wIlL then scRNA-SeQ To Capture the TraNscriptome foR eaCh cell AnD quANtify The aAV anD promoTEr- specIfic barcoDeS In everY Cell
 efficient transgene deliv ery to cla ssifi edneu ro ns.Firs t, we will syn t hesi ze massive libraries o f mut at e d AA V  v ector s and s y nt h e tic  p ro mot er s ,in wh ich  each v ariant ispai re d with a uni q ue  DNA barco de.  We will the n s cRNA-S eq  to captu rethe t ranscr i ptomefor eachce l l andq uantify t he  AAV  and promoter- sp e ci f ic barcodes in  every  c e ll 
 efficient_transgene delivery_to classified neurons. First,_we will_synthesize_massive libraries_of_mutated AAV vectors_and synthetic promoters,_in which each variant_is paired with_a_unique DNA barcode. We will then scRNA-Seq to capture the transcriptome for each cell_and_quantify the_AAV_and_promoter- specific barcodes in every_cell