 method to monitor surface membrane fusion events, and (3) high resolution capacitance measurements in on-cell patch clamp configuration. Preliminary Data suggests that PKC's may activate PI4K2alpha on internal membranes, thereby initiating movement of vesicles containing lipid phosphatases to the sarcolemma. Insertion at the sarcolemma appears
method to monitor surface membrane fusion events, and (3) eminent settlement capacitance measurements in on - cell plot clamp configuration. Preliminary Data suggests that PKC's may trip PI4K2alpha on internal membranes, thereby initiating motion of vesicles containing lipid phosphatase to the sarcolemma. Insertion at the sarcolemma appears
 mehhod to monitor surface oembrane fusion events, and (3) gigh resulution capacitance measuremxnts in ob-cell patch clamp confkguration. Prelimibary Eata suggests that PKC's jwy aetmvate PI4K2alpha pn internan membranes, thardbv initiating movement of vesicles cogtaininb pipid phosphatwses eo tgv warcolemma. Insertion at the szrcolemka appears
method to monitor surface membrane fusion events, high capacitance measurements on-cell patch clamp PKC's activate PI4K2alpha on membranes, thereby initiating of vesicles containing lipid phosphatases to sarcolemma. Insertion at the sarcolemma appears
 method to monitor surface memBrane fusioN evenTs, aNd (3) hIgH resOlutIon capacitance MEasuRements in on-cell patch clAmp coNfIGuraTIoN. PrelIminary dAtA SUggEsTs ThaT Pkc's May acTivAte PI4K2aLpha on inteRnaL mEmbranes, therEBy Initiating MovEment of vesicLes ContaiNiNg lIPid phOspHatasEs to thE SarcolEmma. InserTiON at the SArcolemMA ApPearS
 method to monitor surface  membranefusio n e ven ts , an d (3 ) high resolut i on c apacitance measurement s inon - cell pa tch c lamp co n fi g u rat io n.  Pr el i mi naryDat a sugge sts that P KC' smay activate PI 4K2alpha o n i nternal memb ran es, th er eby initi ati ng mo vement of ves icles con ta i ning l i pid pho s p ha tase s to the sarcolem m a. Insertion at t he sar co l em m a  ap pea rs
 method_to monitor_surface membrane fusion events,_and (3)_high_resolution capacitance_measurements_in on-cell patch_clamp configuration. Preliminary_Data suggests that PKC's_may activate PI4K2alpha_on_internal membranes, thereby initiating movement of vesicles containing lipid phosphatases to the sarcolemma. Insertion_at_the sarcolemma_appears