 have continued to investigate the mechanism of PI3K stimulation by TRbeta and the consequences of PI3K signaling for the physiological effects of thyroid hormone. We have used a fluorescent PIP3 binding domain from the Akt protein kinase coupled with cyan and yellow fluorecent proteins to detect PIP3 production by fluorescence resonance energy transfer (
have continued to investigate the mechanism of PI3 K stimulation by TRbeta and the consequences of PI3 K signaling for the physiologic effect of thyroid hormone. We have used a fluorescent PIP3 bind world from the Akt protein kinase coupled with cyan and yellow fluorecent protein to detect PIP3 production by fluorescence resonance energy transportation (
 hage continued to investigxte the mechanism of PM3K stimhlation cy TRbeta and the consequencxs od PI3K signaling for the phyriologicap effectw of rhyroid hocjone. We have hded c hluorescent PIP3 binding dmmain from the Ayt protein kinase coupled with cyan agd yellpw fluorecent prjteimf to detect PIP3 production by fluorescsnce revonance energu transfer (
have continued to investigate the mechanism of by and the of PI3K signaling thyroid We have used fluorescent PIP3 binding from the Akt protein kinase coupled cyan and yellow fluorecent proteins to detect PIP3 production by fluorescence resonance energy (
 have continued to investigatE the mechanIsm of pI3K StiMuLatiOn by tRbeta and the coNSequEnces of PI3K signaling for The phYsIOlogICaL effeCts of thYRoID HorMoNe. we hAvE UsEd a flUorEscent PiP3 binding dOmaIn From the Akt prOTeIn kinase coUplEd with cyan anD yeLlow flUoRecENt proTeiNs to dEtect Pip3 produCtion by flUoREscencE ResonanCE EnErgy Transfer (
 have continued to investi gate the m echan ism  of  P I3Kstim ulation by TRb e ta a nd the consequences of  PI3K  s i gnal i ng  forthe phy s io l o gic al  e ffe ct s  o f thy roi d hormo ne. We hav e u se d a fluoresc e nt  PIP3 bind ing  domain from  th e Aktpr ote i n kin ase  coup led wi t h cyan  and yell ow fluore c ent pro t e in s to  detect PIP3 prod u ct i on by fluoresc ence r es o na n c e e ner gy transfe r(
 have_continued to_investigate the mechanism of_PI3K stimulation_by_TRbeta and_the_consequences of PI3K_signaling for the_physiological effects of thyroid_hormone. We have_used_a fluorescent PIP3 binding domain from the Akt protein kinase coupled with cyan and_yellow_fluorecent proteins_to_detect_PIP3 production by fluorescence resonance_energy transfer (