 the antibody characterized. The cDNA coding for debranching enzyme from both human liver and muscle will be isolated by screening lambda gtll human cDNA libraries with antibody against porcine debranching enzyme. The debranching enzyme mRNA in these two tissues will be compared by Northern blot analysis, cDNA sequencing and/or sequencing of
the antibody characterized. The cDNA coding for debranching enzyme from both human liver and muscle will be sequester by screen lambda gtll human cDNA libraries with antibody against porcine debranching enzyme. The debranching enzyme mRNA in these two tissues will be compared by Northern smudge analysis, cDNA sequencing and/or sequence of
 thf antibody characterized. The cDNA codiny for dxbranchjng enzyoe from both human liver and myscle will be isolated by szreening pambda grll iuman cDNA libracjes witm antjnody cgeinst porcine dgbranching etzyme. The debrdnzhnng enzyme mRNA in these two tissues will br fompared by Nottherm blof analysis, cDNA sequencing and/or ssquenciig of
the antibody characterized. The cDNA coding for from human liver muscle will be human libraries with antibody porcine debranching enzyme. debranching enzyme mRNA in these two will be compared by Northern blot analysis, cDNA sequencing and/or sequencing of
 the antibody characterized. THe cDNA codiNg for DebRanChIng eNzymE from both human LIver And muscle will be isolateD by scReENing LAmBda gtLl human CdNa LIbrArIeS wiTh ANtIbody AgaInst porCine debranChiNg Enzyme. The debRAnChing enzymE mRnA in these two TisSues wiLl Be cOMpareD by northErn bloT AnalysIs, cDNA seqUeNCing anD/Or sequeNCInG of
 the antibody characterize d. The cDN A cod ing  fo rdebr anch ing enzyme fro m  bot h human liver and musc le wi ll be i s ol atedby scre e ni n g  la mb da  gt ll hu man c DNA  librar ies with a nti bo dy against p o rc ine debran chi ng enzyme. T hedebran ch ing enzym e m RNA i n thes e  two t issues wi ll be com p ared by N or ther n blot analysis,c DN A  sequencing an d/or s eq u en c i ngof
 the_antibody characterized._The cDNA coding for_debranching enzyme_from_both human_liver_and muscle will_be isolated by_screening lambda gtll human_cDNA libraries with_antibody_against porcine debranching enzyme. The debranching enzyme mRNA in these two tissues will be_compared_by Northern_blot_analysis,_cDNA sequencing and/or sequencing of