 specific T3SS inhibitory activity. In Aim 2, we will validate hits identified in Aim 1, using three orthogonal distinct approaches. The identity and structure of bioactive natural products will be determined and initial structure activity analysis performed on identified synthetic scaffolds. Prioritized compounds will be purified or synthesized and evaluated for off target activity,
specific T3SS inhibitory activity. In Aim 2, we will validate hits identified in Aim 1, using three extraneous discrete approaches. The identity and structure of bioactive lifelike products will be compulsive and initial structure activity psychoanalysis performed on identified synthetic scaffold. Prioritized compounds will be purified or synthesize and evaluated for off target bodily process,
 spfcific T3SS inhibitory acuivity. In Aim 2, we will velidate hits iddntified in Aim 1, using three oethoginal distinct approachds. The idvntity ane stcucture of bioacvjve natmxal pdlduccs will be deterkined and hnitial structgrd cctivity analysis performed on identyfied sunhhetic scaffolqs. Pgijritjdeb compounds will be purified or synthevized and evakuated for off target actigity,
specific T3SS inhibitory activity. In Aim 2, validate identified in 1, using three and of bioactive natural will be determined initial structure activity analysis performed on synthetic scaffolds. Prioritized compounds will be purified or synthesized and evaluated for off activity,
 specific T3SS inhibitory actiVity. In Aim 2, wE will ValIdaTe Hits IdenTified in Aim 1, usiNG thrEe orthogonal distinct apProacHeS. the iDEnTity aNd strucTUrE OF biOaCtIve NaTUrAl proDucTs will bE determineD anD iNitial structURe Activity anAlySis performed On iDentifIeD syNThetiC scAffolDs. PrioRItized Compounds WiLL be purIFied or sYNThEsizEd and evaluated for OFf TArget activity,
 specific T3SS inhibitoryactivity.In Ai m 2 , w ewill  val idate hits ide n tifi ed in Aim 1, using thr ee or th o gona l  d istin ct appr o ac h e s.Th eide nt i ty  andstr uctureof bioacti vena tural produc t swill be de ter mined and in iti al str uc tur e  acti vit y ana lysisp erform ed on ide nt i fied s y nthetic s ca ffol ds. Prioritized c o mp o unds will be p urifie do rs y nth esi zed and ev al uated for off ta r g e t a c tivity,
 specific_T3SS inhibitory_activity. In Aim 2,_we will_validate_hits identified_in_Aim 1, using_three orthogonal distinct_approaches. The identity and_structure of bioactive_natural_products will be determined and initial structure activity analysis performed on identified synthetic scaffolds._Prioritized_compounds will_be_purified_or synthesized and evaluated for_off target activity,