- induced visceral hyperalgesia. The second specific aim relates to the characterization of molecular mechanisms linking chronic stress and spinal glial activation. The experimental design proposed in this application includes the behavioral assessment of visceral sensitivity in response to different pharmacological treatments (antagonists, agonists, oligonucleotide antisenses), combined with in vitro analysis using
- induced visceral hyperalgesia. The second specific aim relates to the word picture of molecular mechanism linking chronic stress and spinal glial activation. The experimental purpose proposed in this application admit the behavioral judgment of visceral sensitivity in reaction to different pharmacological treatments (antagonist, agonists, oligonucleotide antisenses), combined with in vitro psychoanalysis using
- infuced visceral hyperalgeria. The second specifir aim rslates tu the characterization of mopexular mechanisms linking chfonic strvss and spinao glial actmbation. Bke exlcrimeutel design propoxed in thiv application hnzlbdes the behavioral assessment of vifceral xejsitivity in rgsponxq to different pharmacological treatmehts (antegonists, agonisys, oligonucleotide antisendes), fombined with in vltro analysus ufung
- induced visceral hyperalgesia. The second specific to characterization of mechanisms linking chronic The design proposed in application includes the assessment of visceral sensitivity in response different pharmacological treatments (antagonists, agonists, oligonucleotide antisenses), combined with in vitro analysis using
- induced visceral hyperalgesIa. The seconD specIfiC aiM rElatEs to The characterizATion Of molecular mechanisms lInkinG cHRoniC StRess aNd spinaL GlIAL acTiVaTioN. THE eXperiMenTal desiGn proposed In tHiS application INcLudes the beHavIoral assessmEnt Of viscErAl sENsitiVitY in reSponse TO diffeRent pharmAcOLogicaL TreatmeNTS (aNtagOnists, agonists, oliGOnUCleotide antiseNses), coMbINeD WIth In vItro analysIs Using
- induced visceral hyperal gesia. The  seco ndspe ci ficaimrelates to the char acterization of molecu lar m ec h anis m slinki ng chro n ic s tre ss  a ndsp i na l gli alactivat ion. The e xpe ri mental desig n  p roposed in  th is applicati oninclud es  th e  beha vio ral a ssessm e nt ofvisceralse n sitivi t y in re s p on se t o different pharm a co l ogical treatme nts (a nt a go n i sts , a gonists, o li gonuc l eotidea nt i s e nse s ), combined w ith in vitr o  an alysis  u sin g 
- induced_visceral hyperalgesia._The second specific aim_relates to_the_characterization of_molecular_mechanisms linking chronic_stress and spinal_glial activation. The experimental_design proposed in_this_application includes the behavioral assessment of visceral sensitivity in response to different pharmacological treatments_(antagonists,_agonists, oligonucleotide_antisenses),_combined_with in vitro analysis using