 cytosolic effects of CTA1 activity, but this work will instead utilize a recently developed biochemical assay that directly monitors the CTA1 translocation event. The assay will be used to (i) identify the structural features of CTA1 that are required for translocation to, and persistence in, the cytosol; (ii) identify
cytosolic effects of CTA1 activity, but this work will instead use a recently modernize biochemical assay that directly monitors the CTA1 translocation consequence. The assay will be used to (one) identify the structural feature of speech of CTA1 that are required for translocation to, and persistence in, the cytosol; (ii) name
 cyhosolic effects of CTA1 agtivity, but this work wmll insfead utiuize a recently developed bilcyemicql assay that directly monitors the CTA1 traislocation event. The assay wilm be bsxd to (i) identifi the structgral features mf CCA1 that are required for translocatijn to, amd persistence ig, tht crtoskl; (ii) identify
cytosolic effects of CTA1 activity, but this instead a recently biochemical assay that event. assay will be to (i) identify structural features of CTA1 that are for translocation to, and persistence in, the cytosol; (ii) identify
 cytosolic effects of CTA1 actiVity, but thiS work WilL inStEad uTiliZe a recently devELopeD biochemical assay that dIrectLy MOnitORs The CTa1 translOCaTIOn eVeNt. the AsSAy Will bE usEd to (i) idEntify the sTruCtUral features OF CtA1 that are rEquIred for transLocAtion tO, aNd pERsistEncE in, thE cytosOL; (ii) ideNtify
 cytosolic effects of CTA1  activity,  butthi s w or k wi ll i nstead utilize a re cently developed bioch emica la ssay th at di rectlym on i t ors  t he  CT A1 tr anslo cat ion eve nt. The as say  w ill be usedt o(i) identi fythe structur alfeatur es  of CTA1tha t are  requi r ed for  transloc at i on to, and per s i st ence  in, the cytosol; (i i ) identify
 cytosolic_effects of_CTA1 activity, but this_work will_instead_utilize a_recently_developed biochemical assay_that directly monitors_the CTA1 translocation event._The assay will_be_used to (i) identify the structural features of CTA1 that are required for translocation_to,_and persistence_in,_the_cytosol; (ii) identify