 of antipyrine and its metabolites, norantipyrine, 4-hydroxyantipyrine, and 3-hydroxyantipyrine. This method involved studies to determine optimal buffer pH and molarity, ratio of solvents in the mobile phase of the system, and effects of centrifugation temperature during assay. Experiments
of antipyrine and its metabolites, norantipyrine, 4 - hydroxyantipyrine, and 3 - hydroxyantipyrine. This method involved studies to settle optimum buffer pH and molarity, ratio of solvent in the fluid phase of the system, and effects of centrifugation temperature during assay. Experiments
 of antipyrine and its metanolites, norantipirune, 4-hyvroxyanfipyrine, and 3-hydroxyantipyrine. This lerhod unvolved studies to degermine oitimal budfer pH and moledity, rabno of dolvznvs in the mobilg phase of tve system, and afweets of centrifugation temperature duwing asxaj. Experiments
of antipyrine and its metabolites, norantipyrine, 4-hydroxyantipyrine, This involved studies determine optimal buffer solvents the mobile phase the system, and of centrifugation temperature during assay. Experiments
 of antipyrine and its metabolItes, norantIpyriNe, 4-hYdrOxYantIpyrIne, and 3-hydroxyaNTipyRine. This method involved StudiEs TO detERmIne opTimal buFFeR Ph anD mOlAriTy, RAtIo of sOlvEnts in tHe mobile phAse Of The system, and EFfEcts of centRifUgation tempeRatUre durInG asSAy. ExpEriMents
 of antipyrine and its met abolites,noran tip yri ne , 4- hydr oxyantipyrine, and3-hydroxyantipyrine. T his m et h od i n vo lvedstudies to d ete rm in e o pt i ma l buf fer  pH and  molarity,  ra ti o of solvent s  i n the mobi lephase of the  sy stem,an d e f fects  of  cent rifuga t ion te mperature  d u ring a s say. Ex p e ri ment s
 of_antipyrine and_its metabolites, norantipyrine, 4-hydroxyantipyrine,_and 3-hydroxyantipyrine._This_method involved_studies_to determine optimal_buffer pH and_molarity, ratio of solvents_in the mobile_phase_of the system, and effects of centrifugation temperature during assay. Experiments