 and the subsequent neurocognitive deficits which occur during ECM using both a monoclonal antibody against phosphorylated tau and tau-knock-out (KO) mouse experiments. AIM2: To determine the role of tau in an in vitro human CM model. We will challenge cultured immortalized human neurons with supernatants obtained from blood-brain
and the subsequent neurocognitive deficits which occur during ECM using both a monoclonal antibody against phosphorylated tau and tau - pink - out (KO) shiner experiments. AIM2: To determine the function of tau in an in vitro human CM mannequin. We will challenge cultured immortalized human neurons with supernatant obtained from blood - brain
 anf the subsequent neuroconnitive deficits which mccur suring EZM using both a monoclonal aitibidy atainst phosphorylated gau and twu-knock-oyt (KI) mouse expxdiments. AIM2: Tk detzrnine the role pf tau in dn in vitro hukav EM model. We will challenge cultured ymmortakixed human neurjns eyth alptrnatants obtained from blood-braih
and the subsequent neurocognitive deficits which occur using a monoclonal against phosphorylated tau AIM2: determine the role tau in an vitro human CM model. We will cultured immortalized human neurons with supernatants obtained from blood-brain
 and the subsequent neurocognItive deficIts whIch OccUr DuriNg ECm using both a monOClonAl antibody against phospHorylAtED tau ANd Tau-knOck-out (Ko) MoUSE exPeRiMenTs. aiM2: to detErmIne the rOle of tau in An iN vItro human CM mODeL. We will chaLleNge cultured iMmoRtalizEd HumAN neurOns With sUpernaTAnts obTained froM bLOod-braIN
 and the subsequent neuroc ognitive d efici tswhi ch  occ ur d uring ECM usin g  bot h a monoclonal antibod y aga in s t ph o sp horyl ated ta u  a n d  ta u- kn ock -o u t(KO)mou se expe riments. A IM2 :To determine th e role oftau  in an in vi tro  human  C M m o del.Wewillchalle n ge cul tured imm or t alized human n e u ro ns w ith supernatantso bt a ined from bloo d-brai n
 and_the subsequent_neurocognitive deficits which occur_during ECM_using_both a_monoclonal_antibody against phosphorylated_tau and tau-knock-out_(KO) mouse experiments. AIM2:_To determine the_role_of tau in an in vitro human CM model. We will challenge cultured immortalized_human_neurons with_supernatants_obtained_from blood-brain