%), but higher than that for intron 5, suggesting that evolutionary drift of intron 4 has been restrained and implying selection for functionally important sequences. We then conducted RT-PCR analyses with placental mRNA using an upstream primer specific for Mamu-AG exon 4, and a downstream primer which would amplify through the 3U-untranslated
%), but higher than that for intron 5, suggesting that evolutionary drift of intron 4 has been restrained and implying survival for functionally authoritative sequences. We then conducted RT - PCR analyses with placental messenger rna using an upstream primer specific for Mamu - AG exon 4, and a downstream primer which would magnify through the 3U - untranslated
%), buh higher than that for iktron 5, suggestiny that xvolutiknary drkft of intron 4 has been restcainwd ane implying selection fur functilnally inporuant sequences. We then cokbuctes RT-PER analyses with placental mRNA using an uoscream primer specific for Mamu-AG exog 4, and s fownstream priier erich would amplify through the 3U-untranalated
%), but higher than that for intron that drift of 4 has been functionally sequences. We then RT-PCR analyses with mRNA using an upstream primer specific Mamu-AG exon 4, and a downstream primer which would amplify through the 3U-untranslated
%), but higher than that for introN 5, suggestinG that EvoLutIoNary DrifT of intron 4 has beEN resTrained and implying seleCtion FoR FuncTIoNally ImportaNT sEQUenCeS. WE thEn COnDucteD RT-pCR analYses with plAceNtAl mRNA using aN UpStream primEr sPecific for MaMu-Ag exon 4, aNd A doWNstreAm pRimer Which wOUld ampLify throuGh THe 3U-untRAnslateD
%), but higher than that f or intron5, su gge sti ng  tha t ev olutionary dri f t of  intron 4 has been res train ed andi mp lying  select i on f orfu nc tio na l ly  impo rta nt sequ ences. Wethe nconducted RT - PC R analyses  wi th placental  mR NA usi ng  an upstr eam  prim er spe c ific f or Mamu-A Ge xon 4, and a d o w ns trea m primer which wo u ld amplify throug h the3U - un t r ans lat ed
%), but_higher than_that for intron 5,_suggesting that_evolutionary_drift of_intron_4 has been_restrained and implying_selection for functionally important_sequences. We then_conducted_RT-PCR analyses with placental mRNA using an upstream primer specific for Mamu-AG exon 4,_and_a downstream_primer_which_would amplify through the 3U-untranslated