 at the Gtl2 promoter and its relationship to monoallelic expression will be investigated. Bisulfite mutagenesis and DNA sequencing will be the primary methodology utilized to examine methylation in these analyses. This research will advance the field of imprinting by determining whether the establishment of imprinting marks is globally or independently controlled. Furthermore,
at the Gtl2 promoter and its relationship to monoallelic expression will be investigated. Bisulfite mutagenesis and deoxyribonucleic acid sequence will be the primary methodology utilized to test methylation in these analysis. This research will advance the playing field of impress by determining whether the constitution of imprinting marks is globally or independently controlled. Furthermore,
 at the Gtl2 promoter and itr relationship jo monoanlelic expresskon will be investigated. Bisnlfire muucgenesis and DNA seqjencing wpll be thw prmmary methodologb utilizcb to swaminz nethylation in these ananyses. This resaafck will advance the field of imprintigg by drtfrmining whethgr tht eftabmpsmment of imprinting marks is glogally og independently cpntrolled. Furthermore,
at the Gtl2 promoter and its relationship expression be investigated. mutagenesis and DNA methodology to examine methylation these analyses. This will advance the field of imprinting determining whether the establishment of imprinting marks is globally or independently controlled. Furthermore,
 at the Gtl2 promoter and its relAtionship tO monoAllEliC eXpreSsioN will be investiGAted. bisulfite mutagenesis anD DNA sEqUEnciNG wIll be The primARy METhoDoLoGy uTiLIzEd to eXamIne methYlation in tHesE aNalyses. This rESeArch will adVanCe the field of ImpRintinG bY deTErminIng WhethEr the eSTablisHment of imPrINting mARks is glOBAlLy or Independently contROlLEd. Furthermore,
 at the Gtl2 promoter andits relati onshi p t o m on oall elic  expression wi l l be  investigated. Bisulfi te mu ta g enes i sand D NA sequ e nc i n g w il lbeth e  p rimar y m ethodol ogy utiliz edto  examine met h yl ation in t hes e analyses.Thi s rese ar chw ill a dva nce t he fie l d of i mprinting  b y  deter m ining w h e th er t he establishmento fi mprinting mark s is g lo b al l y  or  in dependentl ycontr o lled. F u rt h e r mor e ,
 at_the Gtl2_promoter and its relationship_to monoallelic_expression_will be_investigated._Bisulfite mutagenesis and_DNA sequencing will_be the primary methodology_utilized to examine_methylation_in these analyses. This research will advance the field of imprinting by determining whether_the_establishment of_imprinting_marks_is globally or independently controlled._Furthermore,