 hyperinsulinemia in the development of cardiovascular disease. This proposal is based on a fundamentally new approach to the isolation of bacterial virulence genes involved in pathogenesis, termed IVET (in vivo expression technology), that allows for the positive selection of bacterial genes that are specifically induced in host tissues. This methodology will be used to characterize the
hyperinsulinemia in the development of cardiovascular disease. This proposal is based on a fundamentally newfangled overture to the isolation of bacterial virulence genes involved in pathogenesis, termed IVET (in vivo formulation technology), that allows for the positivist excerpt of bacterial genes that are specifically induce in host tissues. This methodology will be use to characterize the
 hyoerinsulinemia in the deyelopment of carbuovascnlar diaease. Thks proposal is based on a fuidamwntaloy new approach to the isolatioj of bacreriel virulence genxa involyzd in iathoyeiesis, termed IVGT (in vivo efpression techtouoyy), that allows for the positive selestion og hacterial genef thse ars specifically induced in host tisaues. Thps methodology wikl be used to characterize the
hyperinsulinemia in the development of cardiovascular disease. is on a new approach to genes in pathogenesis, termed (in vivo expression that allows for the positive selection bacterial genes that are specifically induced in host tissues. This methodology will be to characterize the
 hyperinsulinemia in the deveLopment of cArdioVasCulAr DiseAse. THis proposal is bASed oN a fundamentally new apprOach tO tHE isoLAtIon of BacteriAL vIRUleNcE gEneS iNVoLved iN paThogeneSis, termed IvET (In Vivo expressiON tEchnology), tHat Allows for the PosItive sElEctIOn of bActErial Genes tHAt are sPecificalLy INduced IN host tiSSUeS. ThiS methodology will bE UsED to characterizE the
 hyperinsulinemia in the d evelopment  of c ard iov as cula r di sease. This pr o posa l is based on a fundam ental ly newa pp roach  to the is o l ati on  o f b ac t er ial v iru lence g enes invol ved  i n pathogenes i s,  termed IV ET(in vivo exp res sion t ec hno l ogy),  th at al lows f o r thepositivese l ection of bact e r ia l ge nes that are spec i fi c ally induced i n host  t i ss u e s.Thi s methodol og y wil l  be use d  t o c har a cterize the
 hyperinsulinemia_in the_development of cardiovascular disease._This proposal_is_based on_a_fundamentally new approach_to the isolation_of bacterial virulence genes_involved in pathogenesis,_termed_IVET (in vivo expression technology), that allows for the positive selection of bacterial genes_that_are specifically_induced_in_host tissues. This methodology will_be used to characterize the