 using cultured epithelial cells of renal origin in which we will investigate, using biochemical, biophysical and ultrastructural methods, how tight junctions are formed and plasma membrane polarization is established during monolayer formation and after junctions are opened by experimental procedures. 2. In the same cell system production of enveloped viruses occurs in a polarized
using cultured epithelial cells of renal beginning in which we will investigate, use biochemical, biophysical and ultrastructural methods, how tight junctions are form and plasma membrane polarization is established during monolayer formation and after junction are afford by experimental procedures. 2. In the same cell system output of enveloped viruses occur in a polarize
 uslng cultured epithelial gells of renal otitin in which we will investigate, using biochemicel, buophywical and ultrastructufal methofs, how tught hunctions ede formcb and ilasmc nembrane polarlzation is astablished dusivg monolayer formation and after junceions ate opened by expgrimemeal lgogedures. 2. In the same cell system producuion of enveloped firuses occurs in a polarixed
using cultured epithelial cells of renal origin we investigate, using biophysical and ultrastructural formed plasma membrane polarization established during monolayer and after junctions are opened by procedures. 2. In the same cell system production of enveloped viruses occurs in polarized
 using cultured epithelial ceLls of renal OrigiN in WhiCh We wiLl inVestigate, using BIochEmical, biophysical and ulTrastRuCTuraL MeThods, How tighT JuNCTioNs ArE foRmED aNd plaSma MembranE polarizatIon Is Established dURiNg monolayeR foRmation and afTer JunctiOnS arE OpeneD by ExperImentaL ProcedUres. 2. In the SaME cell sYStem proDUCtIon oF enveloped viruses OCcURs in a polarized
 using cultured epithelial  cells ofrenal  or igi nin w hich  we will inves t igat e, using biochemical,bioph ys i cala nd  ultr astruct u ra l met ho ds , h ow ti ght j unc tions a re formedand  p lasma membra n epolarizati onis establish edduring  m ono l ayerfor matio n anda fter j unctionsar e  opene d  by exp e r im enta l procedures. 2.I nt he same cell s ystempr o du c t ion  of  enveloped  v iruse s  occurs in a pol a rized
 using_cultured epithelial_cells of renal origin_in which_we_will investigate,_using_biochemical, biophysical and_ultrastructural methods, how_tight junctions are formed_and plasma membrane_polarization_is established during monolayer formation and after junctions are opened by experimental procedures. 2._In_the same_cell_system_production of enveloped viruses occurs_in a polarized