 be small under steady state conditions. Therefore, we will perturb the system by inducing oxidation of membranes in vitro (using primarily endogenous oxidants) in order to amplify existing differences. Our preliminary studies show that mitochondrial cholesterol is more oxidizable in PD than in controls. Mitochondrial and synaptosomal fractions will be oxidized by
be small under steady state conditions. consequently, we will perturb the arrangement by inducing oxidation of membranes in vitro (use chiefly endogenous oxidants) in order to amplify exist differences. Our preliminary studies prove that mitochondrial cholesterol is more oxidizable in PD than in controls. Mitochondrial and synaptosomal fraction will be oxidized by
 be small under steady statt conditions. Thergfire, we will lerturb ghe system by inducing oxidavion of mtibranes in vitro (uring primwrily eneogeious oxidants) in order to ampljny exnsving differencex. Our prelhminary studiev rhlw that mitochondrial cholesterol if more pxldizable in PD tham in dontrols. Mitochondrial and synaptoaomal fgactions will be pxidized by
be small under steady state conditions. Therefore, perturb system by oxidation of membranes oxidants) order to amplify differences. Our preliminary show that mitochondrial cholesterol is more in PD than in controls. Mitochondrial and synaptosomal fractions will be oxidized by
 be small under steady state coNditions. ThErefoRe, wE wiLl PertUrb tHe system by induCIng oXidation of membranes in vItro (uSiNG priMArIly enDogenouS OxIDAntS) iN oRdeR tO AmPlify ExiSting diFferences. OUr pReLiminary studIEs Show that miTocHondrial cholEstErol is MoRe oXIdizaBle In PD tHan in cONtrols. mitochondRiAL and syNAptosomAL FrActiOns will be oxidized BY
 be small under steady sta te conditi ons.The ref or e, w e wi ll perturb the syst em by inducing oxidati on of  m e mbra n es  in v itro (u s in g pri ma ri lyen d og enous  ox idants)  in ordertoam plify existi n gdifference s.Our prelimin ary  studi es  sh o w tha t m itoch ondria l  chole sterol is  m o re oxi d izablei n  P D th an in controls. M i to c hondrial and s ynapto so m al f rac tio ns will be  o xidiz e d by
 be_small under_steady state conditions. Therefore,_we will_perturb_the system_by_inducing oxidation of_membranes in vitro_(using primarily endogenous oxidants)_in order to_amplify_existing differences. Our preliminary studies show that mitochondrial cholesterol is more oxidizable in PD_than_in controls._Mitochondrial_and_synaptosomal fractions will be oxidized_by