 enzymes involved. The enzyme proteins involved in each reaction will be purified and the substrate affinities and substrate range will be studied for each step. Physiologic and pharmacologic controls of conjugation will be studied by assaying the individual reactions. Initial work will be performed in animal models as a prerequisite for future study in human tissue.
enzymes involved. The enzyme proteins involved in each chemical reaction will be purify and the substrate affinities and substrate range will be study for each footstep. Physiologic and pharmacologic controls of junction will be studied by assaying the individual chemical reaction. Initial work will be performed in animal model as a prerequisite for future study in human tissue.
 enxymes involved. The enzymt proteins involvgd in earh reacfion wilu be purified and the substrete qffinuties and substrate ravge will he studiwd fie each ste'. Physiologic zkd phcrnacologic conttols of conjggation will ba rtbdied by assaying the individual reastions. Onltial work wilj be [erfkgmtd in animal models as a prerequiaite fog future study in human tissue.
enzymes involved. The enzyme proteins involved in will purified and substrate affinities and for step. Physiologic and controls of conjugation be studied by assaying the individual Initial work will be performed in animal models as a prerequisite for future in human tissue.
 enzymes involved. The enzyme pRoteins invOlved In eAch ReActiOn wiLl be purified anD The sUbstrate affinities and sUbstrAtE RangE WiLl be sTudied fOR eACH stEp. phYsiOlOGiC and pHarMacologIc controls Of cOnJugation will BE sTudied by asSayIng the indiviDuaL reactIoNs. INItial WorK will Be perfORmed in Animal modElS As a preREquisitE FOr FutuRe study in human tisSUe.
 enzymes involved. The enz yme protei ns in vol ved  i n ea ch r eaction will b e  pur ified and the substrat e aff in i ties an d sub strater an g e  wi ll  b e s tu d ie d for  ea ch step . Physiolo gic  a nd pharmacol o gi c controls  of  conjugation  wi ll best udi e d byass aying  the i n dividu al reacti on s . Init i al work w il l be  performed in ani m al models as a pr erequi si t ef o r f utu re study i nhuman tissue. 
 enzymes_involved. The_enzyme proteins involved in_each reaction_will_be purified_and_the substrate affinities_and substrate range_will be studied for_each step. Physiologic_and_pharmacologic controls of conjugation will be studied by assaying the individual reactions. Initial work_will_be performed_in_animal_models as a prerequisite for_future study in human tissue.