 be used to follow the fate of internalized viral filaments and examine whether budding viral filaments can mediate cell-to-cell spread of infection. In specific aim 2, we will focus on the pathogenic potential of filamentous influenza virus. Using primary human nasopharyngeal organ and epithelial cultures we will determine whether infection is restricted to
be used to follow the fate of internalized viral filaments and test whether bud viral filaments can mediate cell - to - cellular telephone spread of infection. In specific aim 2, we will concentrate on the pathogenic potential of filamentous influenza virus. use primary human nasopharyngeal organ and epithelial culture we will determine whether infection is restrict to
 be used to follow the fate of internalizeb viral filamsnts and examine whether budding virel fulamebts can mediate cell-to-zell sprewd of indectmon. In specific ejm 2, we will fkgus ou vhe pathogenic kotential of filamentous itfuuznza virus. Using primary human nasopraryngesl organ and epijhelisj cumnuves we will determine whether inrection is restrictec to
be used to follow the fate of filaments examine whether viral filaments can In aim 2, we focus on the potential of filamentous influenza virus. Using human nasopharyngeal organ and epithelial cultures we will determine whether infection is restricted
 be used to follow the fate of inTernalized Viral FilAmeNtS and ExamIne whether buddINg viRal filaments can mediate Cell-tO-cELl spREaD of inFection. iN sPECifIc AiM 2, we WiLL fOcus oN thE pathogEnic potentIal Of Filamentous iNFlUenza virus. usiNg primary humAn nAsophaRyNgeAL orgaN anD epitHelial CUltureS we will deTeRMine whETher infECTiOn is Restricted to
 be used to follow the fat e of inter naliz edvir al  fil amen ts and examine whet her budding viral fila ments  c a n me d ia te ce ll-to-c e ll s pre ad  o f i nf e ct ion.Inspecifi c aim 2, w e w il l focus on t h epathogenic  po tential of f ila mentou sinf l uenza  vi rus.Usingp rimary  human na so p haryng e al orga n an d ep ithelial cultures we will determine  wheth er in f e cti onis restric te d to
 be_used to_follow the fate of_internalized viral_filaments_and examine_whether_budding viral filaments_can mediate cell-to-cell_spread of infection. In_specific aim 2,_we_will focus on the pathogenic potential of filamentous influenza virus. Using primary human nasopharyngeal_organ_and epithelial_cultures_we_will determine whether infection is_restricted to