 formed by shotgun recombinant DNA phages for those with inserts complementary to probe sequences. After these clones are amplified those with functional HPRT genes will be identified by transfer of their DNA into HPRT- cells using micro-injection or calcium-phosphate precipitation techniques. If the approach outlined above is successful we will attempt to
formed by shotgun recombinant DNA phages for those with inserts complemental to probe sequence. After these clones are amplified those with running HPRT genes will be identify by transfer of their deoxyribonucleic acid into HPRT- cells using micro - injection or calcium - phosphate precipitation technique. If the approach outlined above is successful we will undertake to
 fogmed by shotgun recombinxnt DNA phages yir thove witg insertr complementary to probe seqnencws. Afuvr these clones are aoplified nhose wity fuictional HPRT geiss will be idsktifizd by transfer on their DNA into HPRT- celns uding micro-injection or calcium-phosprate prrclpitation techgiquts. Yf tgv cpproach outlined above is succsssful xe will attempt to
formed by shotgun recombinant DNA phages for inserts to probe After these clones HPRT will be identified transfer of their into HPRT- cells using micro-injection or precipitation techniques. If the approach outlined above is successful we will attempt to
 formed by shotgun recombinanT DNA phages For thOse WitH iNserTs coMplementary to pRObe sEquences. After these clonEs are AmPLifiED tHose wIth funcTIoNAL HPrT GeNes WiLL bE idenTifIed by trAnsfer of thEir dNa into HPRT- celLS uSing micro-iNjeCtion or calciUm-pHosphaTe PreCIpitaTioN techNiques. iF the apProach outLiNEd abovE Is succeSSFuL we wIll attempt to
 formed by shotgun recombi nant DNA p hages  fo r t ho se w ithinserts comple m enta ry to probe sequences.  Afte rt hese cl onesare amp l if i e d t ho se  wi th fu nctio nal  HPRT g enes willbeid entified byt ra nsfer of t hei r DNA into H PRT - cell susi n g mic ro- injec tion o r  calci um-phosph at e  preci p itation t ec hniq ues. If the appro a ch outlined above  is su cc e ss f u l w e w ill attemp tto
 formed_by shotgun_recombinant DNA phages for_those with_inserts_complementary to_probe_sequences. After these_clones are amplified_those with functional HPRT_genes will be_identified_by transfer of their DNA into HPRT- cells using micro-injection or calcium-phosphate precipitation techniques._If_the approach_outlined_above_is successful we will attempt_to