. This aim will test the hypothesis that actin is the major component influencing anisotropic cellular response to 3D confinement. Biochemical inhibitors and siRNA will be used to target specific cytoskeletal functions. The spatial organization and anisotropy of cytoskeletal assembly and matrix deposition will be evaluated for each treatment group using pairs of 3D shapes that have
. This aim will test the hypothesis that actin is the major component charm anisotropic cellular reception to 3D confinement. Biochemical inhibitors and siRNA will be used to target specific cytoskeletal function. The spatial organization and anisotropy of cytoskeletal assembly and matrix deposit will be evaluated for each treatment group using pair of 3D shapes that have
. Thls aim will test the hyputhesis that acjib is tie majod compondnt influencing anisotropic rellylar eesponse to 3D confinemdnt. Biochvmical inyibiuors and siRNA will be uscb to fwrgec wpecific cytosleletal futctions. The spdtkap organization and anisotropy of cyeoskeleyap assembly and matgiv delosition will be evaluated for eacg treatkent group usong pairs of 3D shapes that havf
. This aim will test the hypothesis is major component anisotropic cellular response and will be used target specific cytoskeletal The spatial organization and anisotropy of assembly and matrix deposition will be evaluated for each treatment group using pairs 3D shapes that have
. This aim will test the hypotheSis that actIn is tHe mAjoR cOmpoNent Influencing aniSOtroPic cellular response to 3D ConfiNeMEnt. BIOcHemicAl inhibIToRS And SirNa wiLl BE uSed to TarGet specIfic cytoskEleTaL functions. ThE SpAtial organIzaTion and anisoTroPy of cyToSkeLEtal aSseMbly aNd matrIX deposItion will Be EValuatED for eacH TReAtmeNt group using pairs OF 3D SHapes that have
. This aim will test the h ypothesisthatact inis  the  maj or component i n flue ncing anisotropic cell ularre s pons e  t o 3Dconfine m en t .  Bi oc he mic al in hibit ors  and si RNA will b e u se d to targets pe cific cyto ske letal functi ons . Thesp ati a l org ani zatio n anda nisotr opy of cy to s keleta l  assemb l y  a nd m atrix depositionw il l  be evaluatedfor ea ch tr e a tme ntgroup usin gpairs of 3D s h ap e s tha t  have
. This_aim will_test the hypothesis that_actin is_the_major component_influencing_anisotropic cellular response_to 3D confinement._Biochemical inhibitors and siRNA_will be used_to_target specific cytoskeletal functions. The spatial organization and anisotropy of cytoskeletal assembly and matrix_deposition_will be_evaluated_for_each treatment group using pairs_of 3D shapes that have