 are monitored under alternating depleting and nondepleting conditions. A physilogically based mathematical relationship relates tissue perfusion to the PO2 and PCO2 measurements. This proposal is directed toward the in vivo validation of the method. We have developed a method for preparing a covalent conjugate of proteins with purified gang
are monitored under alternating depleting and nondepleting conditions. A physilogically free-base numerical relationship relates tissue perfusion to the PO2 and PCO2 measurement. This proposal is conduct toward the in vivo validation of the method. We have develop a method for preparing a covalent conjugate solution of protein with purified gang
 arf monitored under alternxting depleting and noidepletjng condktions. A physilogically basev marhemaupcal relationship relxtes tissle perfusuon uo the PO2 and PCO2 measurements. Fmis pxo'osal is directgd toward tha in vivo valigagiln of the method. We have developed w methoc vor preparing w conajent bokjugate of proteins with purifies gang
are monitored under alternating depleting and nondepleting physilogically mathematical relationship tissue perfusion to This is directed toward in vivo validation the method. We have developed a for preparing a covalent conjugate of proteins with purified gang
 are monitored under alternatIng depletiNg and NonDepLeTing CondItions. A physiloGIcalLy based mathematical relAtionShIP relATeS tissUe perfuSIoN TO thE Po2 aNd PcO2 MEaSuremEntS. This prOposal is diRecTeD toward the in VIvO validatioN of The method. We hAve DeveloPeD a mEThod fOr pReparIng a coVAlent cOnjugate oF pROteins WIth puriFIEd Gang
 are monitored under alter nating dep letin g a ndno ndep leti ng conditions. A ph ysilogically based mat hemat ic a l re l at ionsh ip rela t es t iss ue  p erf us i on  to t hePO2 and  PCO2 meas ure me nts. This pr o po sal is dir ect ed toward th e i n vivo  v ali d ation  of  themethod .  We ha ve develo pe d  a met h od forp r ep arin g a covalent conj u ga t e of proteinswith p ur i fi e d  ga ng
 are_monitored under_alternating depleting and nondepleting_conditions. A_physilogically_based mathematical_relationship_relates tissue perfusion_to the PO2_and PCO2 measurements. This_proposal is directed_toward_the in vivo validation of the method. We have developed a method for preparing_a_covalent conjugate_of_proteins_with purified gang