 The second aim will optimize transduction cultures of expanded cells and subsequent clinical studies will incorporate these conditions to use marked expanded cells in patients. This will allow the evaluation of the contribution of expanded cells to long to engraftment. Correlative analysis will be performed comparing the contribution of the marked cells in patients with in vitro assays of
The second aim will optimize transduction cultures of expanded cells and subsequent clinical report will integrate these conditions to use distinguish expand cells in patients. This will admit the evaluation of the contribution of boom cells to retentive to engraftment. Correlative analysis will be performed compare the contribution of the marked cells in affected role with in vitro assay of
 Thf second aim will optimiee transduction colrures mf expznded ceuls and subsequent clinical dtydies will incorporate thesd conditilns to uwe merked expanded cxmls in ictienfd. Thns will allow thg evaluation of the contritugiln of expanded cells to long to engwaftmeny. Forrelative anwlysps wilm be performed comparing the contrjbution of the markec cells in patients with ij vihro assays of
The second aim will optimize transduction cultures cells subsequent clinical will incorporate these cells patients. This will the evaluation of contribution of expanded cells to long engraftment. Correlative analysis will be performed comparing the contribution of the marked cells patients with in vitro assays of
 The second aim will optimize tRansductioN cultUreS of ExPandEd ceLls and subsequeNT cliNical studies will incorpOrate ThESe coNDiTions To use maRKeD EXpaNdEd CelLs IN pAtienTs. THis will Allow the evAluAtIon of the contRIbUtion of expAndEd cells to lonG to EngrafTmEnt. cOrrelAtiVe anaLysis wILl be peRformed coMpARing thE ContribUTIoN of tHe marked cells in paTIeNTs with in vitro aSsays oF
 The second aim will optim ize transd uctio n c ult ur es o f ex panded cells a n d su bsequent clinical stud ies w il l  inc o rp orate  thesec on d i tio ns  t o u se ma rkedexp anded c ells in pa tie nt s. This will al low the ev alu ation of the  co ntribu ti ono f exp and ed ce lls to long t o engraft me n t. Cor r elative a na lysi s will be perform e dc omparing the c ontrib ut i on o f t hemarked cel ls  in p a tientsw it h i n v i tro assays of 
 The_second aim_will optimize transduction cultures_of expanded_cells_and subsequent_clinical_studies will incorporate_these conditions to_use marked expanded cells_in patients. This_will_allow the evaluation of the contribution of expanded cells to long to engraftment. Correlative_analysis_will be_performed_comparing_the contribution of the marked_cells in patients with in_vitro assays_of