 interconversion enzymes will be purified to see whether they occur in an enzyme aggregate. The intracellular localization of these enzymes will be determined. Mutants with alterations in the function or regulation of proteases will be isolated and characterized both with respect to genetics and biochemistry. The consequences to the cell of such alterations in protease function will be
interconversion enzymes will be purified to see whether they occur in an enzyme sum. The intracellular localization of function of these enzyme will be determined. Mutants with alterations in the routine or regulation of proteases will be isolate and characterize both with respect to genetics and biochemistry. The consequence to the cell of such alterations in protease routine will be
 inherconversion enzymes wiul be purified jo see wiether fhey occjr in an enzyme aggregate. Thx inrraceolular localization of these endymes wilo be eetermined. Mutants with zpterctmons in the fungtion or reculation of prmtdades will be isolated and characterised botn aith respect tj gemqtica and biochemistry. The consequencea to tht cell of such altrrations in protease functlon aill be
interconversion enzymes will be purified to see occur an enzyme The intracellular localization determined. with alterations in function or regulation proteases will be isolated and characterized with respect to genetics and biochemistry. The consequences to the cell of such in protease function will be
 interconversion enzymes wilL be purifieD to seE whEthEr They OccuR in an enzyme aggREgatE. The intracellular localIzatiOn OF theSE eNzymeS will be DEtERMinEd. muTanTs WItH alteRatIons in tHe function Or rEgUlation of proTEaSes will be iSolAted and charaCteRized bOtH wiTH respEct To genEtics aND biochEmistry. ThE cONsequeNCes to thE CElL of sUch alterations in pROtEAse function wilL be
 interconversion enzymes w ill be pur ified  to  se ewhet herthey occur ina n en zyme aggregate. The in trace ll u larl oc aliza tion of th e s e e nz ym eswi l lbe de ter mined.Mutants wi thal terations in th e function  or  regulationofprotea se s w i ll be  is olate d andc haract erized bo th with r e spect t o ge neti cs and biochemist r y. The consequenc es toth e  c e l l o f s uch altera ti ons i n  protea s ef u n cti o n will be
 interconversion_enzymes will_be purified to see_whether they_occur_in an_enzyme_aggregate. The intracellular_localization of these_enzymes will be determined._Mutants with alterations_in_the function or regulation of proteases will be isolated and characterized both with respect_to_genetics and_biochemistry._The_consequences to the cell of_such alterations in protease function_will be