 and unmethylated DNA ligands? to create detailed maps of the effect of methylation on binding affinity for a broad panel of human transcription factors from various structural families. Second, we will perform detailed computational analyses and follow-up experiments to test the hypothesis that methylation causes local changes in DNA shape, which in turn modify TF binding
and unmethylated DNA ligands? to create detailed maps of the effect of methylation on bind affinity for a wide panel of human transcription factors from respective structural families. Second, we will do detailed computational analyses and follow - up experiments to screen the hypothesis that methylation causes local change in DNA shape, which in turn modify TF binding
 anf unmethylated DNA liganas? to create dejauled meps of fhe effezt of methylation on binding adfiniuj for a broad panel ow human tganscriptuon hactors from varmkus strmeturam famnlmes. Second, we wlll perform detailed compgtxtnonal analyses and follow-up experimegts to yedt the hypothefis uhae mefhylation causes local changes in SNA shake, which in turn mpdify TF binding
and unmethylated DNA ligands? to create detailed the of methylation binding affinity for transcription from various structural Second, we will detailed computational analyses and follow-up experiments test the hypothesis that methylation causes local changes in DNA shape, which in modify TF binding
 and unmethylated DNA ligands? To create deTaileD maPs oF tHe efFect Of methylation oN BindIng affinity for a broad paNel of HuMAn trANsCriptIon factORs FROm vArIoUs sTrUCtUral fAmiLies. SecOnd, we will pErfOrM detailed comPUtAtional anaLysEs and follow-uP exPerimeNtS to TEst thE hyPotheSis thaT MethylAtion causEs LOcal chANges in Dna ShApe, wHich in turn modify Tf BiNDing
 and unmethylated DNA liga nds? to cr eatedet ail ed  map s of  the effect of meth ylation on binding aff inity  f o r ab ro ad pa nel ofh um a n  tr an sc rip ti o nfacto rsfrom va rious stru ctu ra l families.S ec ond, we wi llperform deta ile d comp ut ati o nal a nal ysesand fo l low-up  experime nt s  to te s t the h y p ot hesi s that methylatio n  c a uses local cha nges i nD NA s hap e,which in t ur n mod i fy TF b i nd i n g 
 and_unmethylated DNA_ligands? to create detailed_maps of_the_effect of_methylation_on binding affinity_for a broad_panel of human transcription_factors from various_structural_families. Second, we will perform detailed computational analyses and follow-up experiments to test the_hypothesis_that methylation_causes_local_changes in DNA shape, which_in turn modify TF binding