 sequencing technology to show that the in vivo pattern of nucleosome positioning can be recapitulated in vitro across the S. cerevisiae genome by chromatin remodelers. Here we propose to develop a genome- wide immobilized template assay that will allow us to ask fundamental questions regarding nucleosome dynamics, transcription factor occupancy, and RNA transcription
sequencing technology to show that the in vivo pattern of nucleosome placement can be reprise in vitro across the S. cerevisiae genome by chromatin remodelers. Here we propose to develop a genome- broad trap template assay that will allow us to ask cardinal questions regarding nucleosome dynamics, arrangement factor occupancy, and RNA recording
 seeuencing technology to smow that the in ruvo pavtern or nucleorome positioning can be reca'ituoated in vitro across the S. cerevisiwe genomw by xhromatin csmodelevf. Hedc we 'ripose to develpp a genoma- wide immobilhzdd template assay that will allow us eo ask gujdamental quesjions wegadding nucleosome dynamics, transcriltion fector occupancy, and RNA transcription
sequencing technology to show that the in of positioning can recapitulated in vitro by remodelers. Here we to develop a wide immobilized template assay that will us to ask fundamental questions regarding nucleosome dynamics, transcription factor occupancy, and RNA
 sequencing technology to shoW that the in Vivo pAttErn Of NuclEosoMe positioning cAN be rEcapitulated in vitro acrOss thE S. CErevISiAe genOme by chROmATIn rEmOdEleRs. hErE we prOpoSe to devElop a genomE- wiDe Immobilized tEMpLate assay tHat Will allow us tO asK fundaMeNtaL QuestIonS regaRding nUCleosoMe dynamicS, tRAnscriPTion facTOR oCcupAncy, and RNA transcrIPtIOn
 sequencing technology toshow thatthe i n v ivo  p atte rn o f nucleosome p o siti oning can be recapitul atedin vitr o  a cross  the S. ce r e vis ia egen om e  b y chr oma tin rem odelers. H ere  w e propose to de velop a ge nom e- wide immo bil ized t em pla t e ass aythatwill a l low us  to ask f un d amenta l  questi o n srega rding nucleosomed yn a mics, transcri ptionfa c to r occ upa ncy, and R NA  tran s criptio n 
 sequencing_technology to_show that the in_vivo pattern_of_nucleosome positioning_can_be recapitulated in_vitro across the_S. cerevisiae genome by_chromatin remodelers. Here_we_propose to develop a genome- wide immobilized template assay that will allow us to_ask_fundamental questions_regarding_nucleosome_dynamics, transcription factor occupancy, and_RNA transcription