 how their biosynthesis and degradation are regulated, their physiologic functions, how they act in vivo, and the structure of the various biosynthetic enzymes. For this purpose we have constructed null mutants in each of the biosynthetic steps in both Escherichia coli and Saccharomyces cerevisiae, and have prepared overexpression systems for the biosynthetic enzymes
how their biosynthesis and degradation are regulated, their physiologic functions, how they act in vivo, and the social organization of the diverse biosynthetic enzymes. For this purpose we have construct null mutant in each of the biosynthetic steps in both Escherichia coli and Saccharomyces cerevisiae, and have prepared overexpression arrangement for the biosynthetic enzymes
 hoa their biosynthesis and degradation arg eegulaved, thejr physiulogic functions, how they acv in vivo, and the structure of ghe variols biosynrhetmc enzymes. For tijs purpose we mave eoistructed null kutants in each of the bhoryuthetic steps in both Escherichia coji and Xafcharomyces cetevispaq, ans have prepared overexpression sysfems fog the biosynthetiv enzymes
how their biosynthesis and degradation are regulated, functions, they act vivo, and the enzymes. this purpose we constructed null mutants each of the biosynthetic steps in Escherichia coli and Saccharomyces cerevisiae, and have prepared overexpression systems for the biosynthetic
 how their biosynthesis and deGradation aRe regUlaTed, ThEir pHysiOlogic functionS, How tHey act in vivo, and the struCture Of THe vaRIoUs bioSynthetIC eNZYmeS. FOr ThiS pURpOse we HavE constrUcted null mUtaNtS in each of the BIoSynthetic sTepS in both EscheRicHia colI aNd SACcharOmyCes ceRevisiAE, and haVe prepareD oVErexprESsion sySTEmS for The biosynthetic enZYmES
 how their biosynthesis an d degradat ion a rereg ul ated , th eir physiologi c  fun ctions, how they act i n viv o, andt he  stru cture o f  t h e  va ri ou s b io s yn theti c e nzymes.  For thispur po se we have c o ns tructed nu llmutants in e ach  of th ebio s ynthe tic  step s in b o th Esc herichiaco l i andS accharo m y ce s ce revisiae, and hav e  p r epared overexp ressio ns ys t e msfor  the biosy nt hetic enzymes 
 how_their biosynthesis_and degradation are regulated,_their physiologic_functions,_how they_act_in vivo, and_the structure of_the various biosynthetic enzymes._For this purpose_we_have constructed null mutants in each of the biosynthetic steps in both Escherichia coli_and_Saccharomyces cerevisiae,_and_have_prepared overexpression systems for the_biosynthetic enzymes