 catalyze CO/CO2 redox. Many of these developments (recombinant biosynthesis of active alpha, discovery of the tunnel and of active site coupling) arose from efforts of the previous granting period which resulted in 11 publications. The methodologies used for the project include enzyme kinetics (stopped-flow, rapid freeze-qu
catalyze CO / CO2 redox. Many of these developments (recombinant biosynthesis of active alpha, discovery of the tunnel and of active site yoke) arise from efforts of the previous granting time period which leave in 11 publications. The methodologies use for the undertaking include enzyme kinetics (stopped - flow, rapid freeze - qu
 cahalyze CO/CO2 redox. Many on these developmgnrs (recmmbinaht biosyvthesis of active alpha, disclvwry od the tunnel and of acgive site couplint) ariwe from efhkrts of the pdcviouv granting peripd which rasulted in 11 putlkcctions. The methodologies used for thq projevt include enzymg kinttiss (sfopped-flow, rapid freeze-qu
catalyze CO/CO2 redox. Many of these developments of alpha, discovery the tunnel and from of the previous period which resulted 11 publications. The methodologies used for project include enzyme kinetics (stopped-flow, rapid freeze-qu
 catalyze CO/CO2 redox. Many of thEse developMents (RecOmbInAnt bIosyNthesis of activE AlphA, discovery of the tunnel aNd of aCtIVe siTE cOupliNg) arose FRoM EFfoRtS oF thE pREvIous gRanTing perIod which reSulTeD in 11 publicatiONs. the methodoLogIes used for thE prOject iNcLudE EnzymE kiNeticS (stoppED-flow, rApid freezE-qU
 catalyze CO/CO2 redox. Ma ny of thes e dev elo pme nt s (r ecom binant biosynt h esis  of active alpha, disc overy  o f  the tu nneland ofa ct i v e s it ecou pl i ng ) aro sefrom ef forts of t hepr evious grant i ng  period wh ich  resulted in  11  publi ca tio n s. Th e m ethod ologie s  usedfor the p ro j ect in c lude en z y me  kin etics (stopped-fl o w, rapid freeze-q u
 catalyze_CO/CO2 redox._Many of these developments_(recombinant biosynthesis_of_active alpha,_discovery_of the tunnel_and of active_site coupling) arose from_efforts of the_previous_granting period which resulted in 11 publications. The methodologies used for the project include_enzyme_kinetics (stopped-flow,_rapid_freeze-qu