 modulating pathway-specific ERK activation should facilitate new GPCR drug discovery efforts for treating mental disorders. We propose four aims for the project. Aim 1. Develop G-protein-dependent ERK2 activation pathway assay. We plan to utilize phosphorylated ERK2 interaction with imp7 as signal readout for assay development. We plan to validate
modulating pathway - specific ERK activation should facilitate newfangled GPCR drug discovery feat for treating mental disorders. We aim four aims for the project. bearing 1. Develop G - protein - subject ERK2 activation nerve pathway try. We plan to utilize phosphorylated ERK2 interaction with imp7 as signal readout for assay exploitation. We plan to validate
 mofulating pathway-specific ERK activation should faciljtate ned GPCR drug discovery effortd dor teeating mental disordefs. We proiose four aimw for the pckject. Ali 1. Dsyelop J-protein-dependekt ERK2 actieation pathway arscy. We plan to utilize phosphorylated ERK2 inyegaction with iip7 ax sighal readout for assay development. Se plan to validate
modulating pathway-specific ERK activation should facilitate new discovery for treating disorders. We propose Aim Develop G-protein-dependent ERK2 pathway assay. We to utilize phosphorylated ERK2 interaction with as signal readout for assay development. We plan to validate
 modulating pathway-specific eRK activatIon shOulD faCiLitaTe neW GPCR drug discoVEry eFforts for treating mentaL disoRdERs. We PRoPose fOur aims FOr THE prOjEcT. AiM 1. DEVeLop G-pRotEin-depeNdent ERK2 acTivAtIon pathway asSAy. we plan to utIliZe phosphorylAteD ERK2 inTeRacTIon wiTh iMp7 as sIgnal rEAdout fOr assay deVeLOpment. wE plan to VALiDate
 modulating pathway-specif ic ERK act ivati onsho ul d fa cili tate new GPCRd rugdiscovery efforts fortreat in g  men t al  diso rders.W ep r opo se  f our  a i ms  forthe  projec t. Aim 1.Dev el op G-protein - de pendent ER K2activation p ath way as sa y.W e pla n t o uti lize p h osphor ylated ER K2 intera c tion wi t h  i mp7as signal readout fo r  assay develop ment.We pl a n  to  va lidate
 modulating_pathway-specific ERK_activation should facilitate new_GPCR drug_discovery_efforts for_treating_mental disorders. We_propose four aims_for the project. Aim_1. Develop G-protein-dependent_ERK2_activation pathway assay. We plan to utilize phosphorylated ERK2 interaction with imp7 as signal_readout_for assay_development._We_plan to validate