 and fluorochrome-labeled viral filaments, we will quantitatively examine whether viral filament entry occurs by partial or complete endocytosis or possibly by a phagocytosis-like mechanism. Membrane fusion assays will be used to determine pH requirements and extent of cell fusion of viral filaments. Live cell video microscopy, confocal and electron microscopy will
and fluorochrome - labeled viral filaments, we will quantitatively examine whether viral filament entrance occur by partial or complete endocytosis or possibly by a phagocytosis - like mechanism. Membrane coalition assays will be used to determine pH requirement and extent of cell fusion of viral fibril. Live cell television microscopy, confocal and electron microscopy will
 anf fluorochrome-labeled vival filaments, we will qnantitafively ebamine whether viral filamenv enrry oxcurs by partial or cooplete enfocytosiw or possibly bb a phagocytosjd-likz nechanism. Membtane fusion dssays will be ureb to determine pH requirements and evtent og fell fusion of virsj fimaments. Live cell video microscopy, confocel and electron microscopy will
and fluorochrome-labeled viral filaments, we will quantitatively viral entry occurs partial or complete phagocytosis-like Membrane fusion assays be used to pH requirements and extent of cell of viral filaments. Live cell video microscopy, confocal and electron microscopy will
 and fluorochrome-labeled virAl filamentS, we wiLl qUanTiTatiVely Examine whether VIral Filament entry occurs by pArtiaL oR CompLEtE endoCytosis OR pOSSibLy By A phAgOCyTosis-LikE mechanIsm. MembranE fuSiOn assays will BE uSed to deterMinE pH requiremeNts And extEnT of CEll fuSioN of viRal filAMents. LIve cell viDeO MicrosCOpy, confOCAl And eLectron microscopy WIlL
 and fluorochrome-labeledviral fila ments , w e w il l qu anti tatively exami n e wh ether viral filament e ntryoc c ursb yparti al or c o mp l e teen do cyt os i sor po ssi bly bya phagocyt osi s- like mechani s m.  Membranefus ion assays w ill  be us ed  to deter min e pHrequir e mentsand exten to f cell fusiono f  v iral  filaments. Livec el l  video microsc opy, c on f oc a l  an d e lectron mi cr oscop y  will
 and_fluorochrome-labeled viral_filaments, we will quantitatively_examine whether_viral_filament entry_occurs_by partial or_complete endocytosis or_possibly by a phagocytosis-like_mechanism. Membrane fusion_assays_will be used to determine pH requirements and extent of cell fusion of viral_filaments._Live cell_video_microscopy,_confocal and electron microscopy will