idonic acid to prostanoids will be determined. Western blots of COX-l and COX-2 protein expression and Northern blots of mRNA levels will be used to determine the adaptation response to cyclic strain. In Specific Aim 3, the role of epithelial oxidant/antioxidant balance in the stretch-induced regulation of
idonic acid to prostanoids will be determined. Western blots of COX - l and COX-2 protein expression and northerly smudge of mRNA levels will be used to determine the adaptation answer to cyclic strain. In Specific Aim 3, the role of epithelial oxidant / antioxidant libra in the reach - induced regulation of
idojic acid to prostanoids dill be determiuwd. Wesvern blkts of CUX-l and COX-2 protein expressiln and Borthern blots of mRNA levels wpll be uswd ti determine the adaicatioh res'oise to cyclic sjrain. In Spewific Aim 3, the rulz of epithelial oxidant/antioxidant bwlance on the stretch-inqucec reghlation of
idonic acid to prostanoids will be determined. of and COX-2 expression and Northern be to determine the response to cyclic In Specific Aim 3, the role epithelial oxidant/antioxidant balance in the stretch-induced regulation of
idonic acid to prostanoids wiLl be determIned. WEstErn BlOts oF COX-L and COX-2 protein EXpreSsion and Northern blots oF mRNA LeVEls wILl Be useD to deteRMiNE The AdApTatIoN ReSponsE to Cyclic sTrain. In SpeCifIc aim 3, the role of EPiThelial oxiDanT/antioxidant BalAnce in ThE stREtch-iNduCed reGulatiON of
idonic acid to prostanoids  will be d eterm ine d.We ster n bl ots of COX-l a n d CO X-2 protein expression  andNo r ther n  b lotsof mRNA le v e lswi ll  be  u s ed  to d ete rmine t he adaptat ion  r esponse to c y cl ic strain.  In  Specific Ai m 3 , thero leo f epi the lialoxidan t /antio xidant ba la n ce int he stre t c h- indu ced regulation of 
idonic acid_to prostanoids_will be determined. Western_blots of_COX-l_and COX-2_protein_expression and Northern_blots of mRNA_levels will be used_to determine the_adaptation_response to cyclic strain. In Specific Aim 3, the role of epithelial oxidant/antioxidant balance_in_the stretch-induced_regulation_of