 RIM101 response in S. cerevisiae. In the third Aim we will extend the original fitness profiling by direct selection for S. cerevisiae deletion mutants resistant to the peptide. Public Health Relevance: Due to the declining rate of discovery of new antibiotics and antifungal agents, and spread of resistant strains of pathogens, there is urgent
RIM101 response in S. cerevisiae. In the third Aim we will extend the original fitness profiling by lineal survival for S. cerevisiae deletion mutants insubordinate to the peptide. Public Health Relevance: Due to the refuse rate of discovery of new antibiotic and antifungal agents, and spread of immune strains of pathogens, there be urgent
 RIL101 response in S. cerevisixe. In the third Aim we will sxtend tfe original fitness profilinj by dirext selection for S. cerdvisiae dvletion mytanus resistant to tis peptide. Pubmlc Heclvh Relevance: Dug to the decnining rate of dkseovery of new antibiotics and antifuggal agrnhs, and spread jf rtsiftanf strains of pathogens, there is urfent
RIM101 response in S. cerevisiae. In the we extend the fitness profiling by deletion resistant to the Public Health Relevance: to the declining rate of discovery new antibiotics and antifungal agents, and spread of resistant strains of pathogens, there urgent
 RIM101 response in S. cerevisiae. IN the third AIm we wIll ExtEnD the OrigInal fitness proFIlinG by direct selection for S. CerevIsIAe deLEtIon muTants reSIsTANt tO tHe PepTiDE. PUblic heaLth ReleVance: Due to The DeClining rate oF DiScovery of nEw aNtibiotics anD anTifungAl AgeNTs, and SprEad of ResistANt straIns of pathOgENs, therE Is urgenT
 RIM101 response in S. cer evisiae. I n the  th ird  A im w e wi ll extend theo rigi nal fitness profilingby di re c t se l ec tionfor S.c er e v isi ae  d ele ti o nmutan tsresista nt to thepep ti de. Public H e al th Relevan ce:  Due to thedec lining  r ate of di sco veryof new antibi otics and  a n tifung a l agent s ,  a nd s pread of resistan t  s t rains of patho gens,th e re i s u rge nt
 RIM101_response in_S. cerevisiae. In the_third Aim_we_will extend_the_original fitness profiling_by direct selection_for S. cerevisiae deletion_mutants resistant to_the_peptide. Public Health Relevance: Due to the declining rate of discovery of new antibiotics_and_antifungal agents,_and_spread_of resistant strains of pathogens,_there is urgent