 a powerful biocatalyst for simvastatin biosynthesis. This proposal will examine the following specific aims: AIM 1: Directed Evolution of LovD. We will use directed evolution methods to improve the catalytic efficiencies of LovD towards simvastatin synthesis. We have developed a high throughput screening assay based on the formation
a powerful biocatalyst for simvastatin biosynthesis. This proposal will examine the following specific aim: AIM 1: steer Evolution of LovD. We will use directed evolution method acting to improve the catalytic efficiencies of LovD towards simvastatin deduction. We have developed a high throughput screening assay establish on the formation
 a oowerful biocatalyst for simvastatin biosynthevis. Thjs proporal will examine the followiig specifuc aims: AIM 1: Directed Dvolution of LovD. We xill use directev evolutljn msbhods vo improve the gatalytic exficiencies of LuvB towards simvastatin synthesis. We hwve devrllped a high thtoughkut scrsvnlng assay based on the formation
a powerful biocatalyst for simvastatin biosynthesis. This examine following specific AIM 1: Directed use evolution methods to the catalytic efficiencies LovD towards simvastatin synthesis. We have a high throughput screening assay based on the formation
 a powerful biocatalyst for siMvastatin bIosynTheSis. thIs prOposAl will examine tHE folLowing specific aims: AIM 1: DIrectEd eVoluTIoN of LoVD. We wilL UsE DIreCtEd EvoLuTIoN methOds To improVe the catalYtiC eFficiencies oF loVD towards sImvAstatin synthEsiS. We havE dEveLOped a HigH throUghput SCreeniNg assay baSeD On the fORmation
 a powerful biocatalyst fo r simvasta tin b ios ynt he sis.  Thi s proposal wil l  exa mine the following spe cific  a i ms:A IM  1: D irected Ev o l uti on  o f L ov D .We wi lluse dir ected evol uti on  methods toi mp rove the c ata lytic effici enc ies of  L ovD towar dssimva statin synthe sis. We h av e  devel o ped a h i g hthro ughput screeninga ss a y based on the  forma ti o n
 a_powerful biocatalyst_for simvastatin biosynthesis. This_proposal will_examine_the following_specific_aims: AIM 1:_Directed Evolution of_LovD. We will use_directed evolution methods_to_improve the catalytic efficiencies of LovD towards simvastatin synthesis. We have developed a high_throughput_screening assay_based_on_the formation