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The simple answer is...
Mostly Southern (call it 80/20), but with some Midwestern traits. 
For example, Kentucky's hilly topography with many streams and rivers are typical of the South, but the ""United We Stand Divided We Fall"" motto is totally opposite of most ""State's rights"" mottos in the South.  Economically we area somewhere in between, having a better economy and population growth than most Midwestern states, but still behind true sunbelt states like Georgia, Tennessee or the Carolinas. Food and accent wise we are definatly southern.

As For Louisville,I think that the ""old stock"" native Louisvillians are  midwestern in accent and attitude, but Jefferson's County nearly doubled it's population from 1940-1960 with people mostly from south central and western Kentucky which really altered the culture. Econmoically, Louisville today has less of a heavy industry based economy  city than it use to be, but instead has employment concentrated in a few ""high tech"" automobile factories and in the service industry, making it less Cincinnati and more like Nashville. , April 20."