_:n51df91a5a4b04bf98ee0f60c86a80c4bxb0
<http://schema.org/articleBody>
"The breast cancer susceptibility protein  BRCA2  is essential for recombinational DNA repair  BRCA2 delivers RAD51 to double-stranded DNA  dsDNA  breaks through interaction with eight conserved  approximately 35 amino acid motifs  the BRC repeats  Here we show that the solitary BRC4 promotes assembly of RAD51 onto single-stranded DNA  ssDNA   but not dsDNA  to stimulate DNA strand exchange  BRC4 acts by blocking ATP hydrolysis and thereby maintaining the active ATP-bound form of the RAD51-ssDNA filament  Single-molecule visualization shows that BRC4 does not disassemble RAD51-dsDNA filaments but rather blocks nucleation of RAD51 onto dsDNA  Furthermore  this behavior is manifested by a domain of BRCA2 comprising all eight BRC repeats  These results establish that the BRC repeats modulate RAD51-DNA interaction in two opposing but functionally reinforcing ways  targeting active RAD51 to ssDNA and prohibiting RAD51 nucleation onto dsDNA  Thus  BRCA2 recruits RAD51 to DNA breaks and  we propose  the BRC repeats regulate DNA-binding selectivity "
<https://www.mysciencework.com/publication/show/brc-repeats-brca2-modulate-dna-binding-selectivity-rad51>