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"Figure 1. Fusions of ftz fragments to full-length βGmRNA are cytoplasmic at steady state. (A) A schematic representation of the different constructs that were used in this figure. (B–E) Plasmids containing the indicated constructs were transfected into human U2OS cells. After 14–18 h, the cells were fixed, permeabilized, and stained for mRNA using a FISH probe directed against βG (B,C) or theMHC SSCR (D,E). The cells were imaged (B,D) and mRNA distribution in the cytoplasm and nucleus was quantified (C,E). Each bar represents the average and standard error of three independent experiments, with each experiment consisting of at least 30 cells. Scale bar = 20 µm."
<https://www.semanticscholar.org/paper/Splicing-promotes-the-nuclear-export-of-%CE%B2-globin-by-Akef-Lee/c350c6208317529a40284cd424ae230bbe1aa0e1>