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"ABSTRACT As the world population increases  the need to develop more efficient wastewater treatment systems requires the use of new technologies  Software aided project and optimization of bioreactors and bioprocesses have become a matter of interest in recent years  especially due to the advance in the state-of-the-art of computational resources  This work aimed to perform gas liquid numerical simulations using the Fluent 16 2 software and to validate this model through Particle Image Velocimetry  PIV  and shadow imaging techniques  Eulerian-Eulerian  laminar  tridimensional and transient simulations were carried out  The results for the mass imbalance for the gas and liquid phases  gas volumetric fraction  gas velocity  bubble size  liquid magnitude and upflow velocity and the velocity profiles for the liquid phase were successfully validated against experimental data  Concerning the dispersed phase  it was found a difference of 4 37  for the gas volumetric fraction between experiments and simulations  Simulated results showed a difference for the bubble mean velocity of 1 73  when compared with shadow imaging results  No coalescence was observed along the experiments  and the flow regime was characterized as dispersed bubble flow  Regarding the liquid phase  it was found a difference of 3 2  for the mean velocity  between simulated and PIV results  Simulated and experimental velocity profiles showed a better agreement at the center of the reactor  Some differences were observed in those profiles  due to geometry simplifications assumed in order to get a better mesh  Considering the good agreement between simulation and experiments  the model was considered validated "
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