The main limitations in NMR is that its resolution decreases with proteins that are larger than 25 kDa and is not as detailed as X-ray crystallography. Additionally, protein NMR analysis is quite difficult and can propose multiple solutions from the same NMR spectrum.
The main limitations in NM!R is that its resolution decreases with proteins that are la!rger than 25 kDa and is not as detailed as X-ray crystallography. Additionall!y, protein NMR analysis is quite difficult and can propose multipl?e solutions from the same NMR: spectrum.