cDNA is also used to study gene expression via methods such as RNA-seq or RT-qPCR. For sequencing, RNA must be fragmented due to sequencing platform size limitations. Additionally, second-strand synthesized cDNA must be ligated with adapters that allow cDNA fragments to be PCR amplified and bind to sequencing flow cells. Gene-specific analysis methods commonly use microarrays and RT-qPCR to quantify cDNA levels via fluorometric and other methods.
CdnA is also uSed to Study gene exprEsSion via metHods sucH aS RNA-seq OR rt-qpcR. foR sequencInG, rNA muST Be FrAgmEnTeD due To SEqUeNcing PlaTfoRm size LimITations. addiTIOnALly, sEcond-STrand sYNtheSIZeD CdnA muSt be ligAtEd with ADapTErs thAT allOw CdNa FrAGmENts To be Pcr AMPlifiEd And bInd to seqUencInG fLow CellS. Gene-specific ANalysis MeTHoDs commOnly use microarrays AnD RT-Qpcr To quanTify cdNA levEls via fluoromeTric and otHEr methods.