Marriage thumb|Isabella of Portugal depicting Charles' wife, a 1548 portrait by Titian thumb|The bronze effigies of Charles and Isabella at the Basilica in El Escorial thumb|Don John of Austria, the natural son of Charles during his widowhood On 21 December 1507, Charles was betrothed to 11-year-old Mary, the daughter of King Henry VII of England and younger sister to the future King Henry VIII of England, who was to take the throne in two years. However, the engagement was called off in 1513, on the advice of Cardinal Thomas Wolsey, and Mary was instead married to King Louis XII of France in 1514.
Marriage thumb|Isabella of Portugal depicting Charles' wife, a 1548 portrait by Titian thumb|The bronze effigies of Charles and Isabella at the Basilica in El Escorial thumb|Don John of Austria, the natural son of Charles during his widowhood On 21 December 1507, Charles was betrothed to 11-year-old Mary, the daughter of King Henry VII of England and younger sister to the future King Henry VIII of England, who was to take the throne in two years. However, the engagement was called off in 1513, on the advice of Cardinal Thomas Wolsey, and Mary was instead married to King Louis XII of France in 1514.