Most commercially available genes for GFP and similar fluorescent proteins are around 730 base-pairs long. The natural protein has 238 amino acids. Its molecular mass is 27 kD. Therefore, fusing the GFP gene to the gene of a protein of interest can significantly increase the protein's size and molecular mass, and can impair the protein's natural function or change its location or trajectory of transport within the cell.
Most commercially available genes for GFP and similar fluorescent proteins are around 730 base-pairs long The natural protein has 238 amino acids Its molecular mass is 27 kD Therefore fusing the GFP gene to the gene of a protein of interest can significantly increase the protein's size and molecular mass and can impair the protein's natural function or change its location or trajectory of transport within the cell