The study of karyotypes is made possible by staining. Usually, a suitable dye, such as Giemsa,A preparation which includes the dyes Methylene Blue, Eosin Y and Azure-A,B,C is applied after cells have been arrested during cell division by a solution of colchicine usually in metaphase or prometaphase when most condensed. In order for the Giemsa stain to adhere correctly, all chromosomal proteins must be digested and removed. For humans, white blood cells are used most frequently because they are easily induced to divide and grow in tissue culture.Gustashaw K.M. 1991. Chromosome stains. In The ACT Cytogenetics Laboratory Manual 2nd ed, ed. M.J. Barch. The Association of Cytogenetic Technologists, Raven Press, New York. Sometimes observations may be made on non-dividing (interphase) cells. The sex of an unborn fetus can be predicted by observation of interphase cells (see amniotic centesis and Barr body).
the study of karyotypes is made possible by staining usually a suitable dye such as giemsaa preparation which includes the dyes methylene blue eosin y and azure-abc is applied after cells have been arrested during cell division by a solution of colchicine usually in metaphase or prometaphase when most condensed in order for the giemsa stain to adhere correctly all chromosomal proteins must be digested and removed for humans white blood cells are used most frequently because they are easily induced to divide and grow in tissue culturegustashaw km 1991 chromosome stains in the act cytogenetics laboratory manual 2nd ed ed mj barch the association of cytogenetic technologists raven press new york sometimes observations may be made on non-dividing (interphase) cells the sex of an unborn fetus can be predicted by observation of interphase cells (see amniotic centesis and barr body)