The potential applications surrounding integrated HPLC columns within microfluidic devices have proven expansive over the last 10–15 years. The integration of such columns allows for experiments to be run where materials were in low availability or very expensive, like in biological analysis of proteins. This reduction in reagent volumes allows for new experiments like single-cell protein analysis, which due to size limitations of prior devices, previously came with great difficulty. The coupling of HPLC-chip devices with other spectrometry methods like mass-spectrometry allow for enhanced confidence in identification of desired species, like proteins. Microfluidic chips have also been created with internal delay-lines that allow for gradient generation to further improve HPLC, which can reduce the need for further separations. Some other practical applications of integrated HPLC chips include the determination of drug presence in a person through their hair and the labeling of peptides through reverse phase liquid chromatography.
the potential applications surrounding integrated hplc columns within microfluidic devices have proven expansive over the last 10–15 years the integration of such columns allows for experiments to be run where materials were in low availability or very expensive like in biological analysis of proteins this reduction in reagent volumes allows for new experiments like single-cell protein analysis which due to size limitations of prior devices previously came with great difficulty the coupling of hplc-chip devices with other spectrometry methods like mass-spectrometry allow for enhanced confidence in identification of desired species like proteins microfluidic chips have also been created with internal delay-lines that allow for gradient generation to further improve hplc which can reduce the need for further separations some other practical applications of integrated hplc chips include the determination of drug presence in a person through their hair and the labeling of peptides through reverse phase liquid chromatography