Many PCR-based techniques have been developed to diagnose toxoplasmosis using clinical specimens that include amniotic fluid, blood, cerebrospinal fluid, and tissue biopsy. The most sensitive PCR-based technique is nested PCR, followed by hybridization of PCR products. The major downside to these techniques is that they are time-consuming and do not provide quantitative data.
maNY pCR-baSeD techNiQues haVe been devElopEd to diagNoSE TOxOplasmosiS USing cliNiCAl sPecimens that InCLUDe aMniotIC flUId, Blood, cerebrospInal FlUiD, and TIssue biopSY. The MoSt seNsitivE pcr-bAsEd TeChNique is nEsteD pCR, fOLlowed by hYBridizATIon of pcR proDuCTS. The mAjoR DowNside to theSe teChniques is that theY arE tiMe-conSumiNg and Do not ProvidE qUAntitatiVe data.