Polysaccharides with unprotected vicinal diols or amino sugars (where some hydroxyl groups are replaced with amines) give a positive periodic acid-Schiff stain (PAS). The list of polysaccharides that stain with PAS is long. Although mucins of epithelial origins stain with PAS, mucins of connective tissue origin have so many acidic substitutions that they do not have enough glycol or amino-alcohol groups left to react with PAS.
Polysaccharides with unprotected vicinal diols or amino sugars (where some hydroxyl groups are replaced with amines) give a positive periodic acid-Schiff stain (PAS) The list of polysaccharides that stain with PAS is long. Although mucins of epithelial origins stain with PAS, mucins of connective tissue origin have so many acidic substitutions that .they do not have enough glycol or amino-alcohol groups left to react with PAS.