In cryo-TEM, instability causes specimen drift and many fast acquisitions with accurate image registration is required to preserve high resolution and obtain high signal to noise images. For low SNR data, the best image registration is achieved by cross-correlating all permutations of images in an image stack.
in cryo-tem instability causes specimen drift and many fast acquisitions with accurate image registration is required to preserve high resolution and obtain high signal to noise images for low snr data the best image registration is achieved by cross-correlating all permutations of images in an image stack