In an alternative figure, DNA factories are similar to projectors and DNAs are like as cinematic films passing constantly into the projectors. In the replication factory model, after both DNA helicases for leading strands and lagging strands are loaded on the template DNAs, the helicases run along the DNAs into each other. The helicases remain associated for the remainder of replication process. Peter Meister et al. observed directly replication sites in budding yeast by monitoring green fluorescent protein (GFP)-tagged DNA polymerases α. They detected DNA replication of pairs of the tagged loci spaced apart symmetrically from a replication origin and found that the distance between the pairs decreased markedly by time. This finding suggests that the mechanism of DNA replication goes with DNA factories. That is, couples of replication factories are loaded on replication origins and the factories associated with each other. Also, template DNAs move into the factories, which bring extrusion of the template ssDNAs and new DNAs. Meister's finding is the first direct evidence of replication factory model. Subsequent research has shown that DNA helicases form dimers in many eukaryotic cells and bacterial replication machineries stay in single intranuclear location during DNA synthesis.
In an alternative figure DNA factories are similar to projectors and DNAs are like as cinematic films passing constantly into the projectors In the replication factory model after both DNA helicases for leading strands and lagging strands are loaded on the template DNAs the helicases run along the DNAs into each other The helicases remain associated for the remainder of replication process Peter Meister et al observed directly replication sites in budding yeast by monitoring green fluorescent protein (GFP)-tagged DNA polymerases α They detected DNA replication of pairs of the tagged loci spaced apart symmetrically from a replication origin and found that the distance between the pairs decreased markedly by time This finding suggests that the mechanism of DNA replication goes with DNA factories That is couples of replication factories are loaded on replication origins and the factories associated with each other Also template DNAs move into the factories which bring extrusion of the template ssDNAs and new DNAs Meister's finding is the first direct evidence of replication factory model Subsequent research has shown that DNA helicases form dimers in many eukaryotic cells and bacterial replication machineries stay in single intranuclear location during DNA synthesis