Separation of unsaturated isomers is possible by silver ion complemented thin-layer chromatography. Other separation techniques include high-performance liquid chromatography (with short columns packed with silica gel with bonded phenylsulfonic acid groups whose hydrogen atoms have been exchanged for silver ions). The role of silver lies in its ability to form complexes with unsaturated compounds.
SEpaRAtiOn of UNsaturaTed isomERs is posSible by silveR Ion COMplementEd thin-laYeR chRoMAtoGraphy other separATioN teChnIQuEs inclUDe HigH-peRFormancE liquid chromatOgRapHy (with sHOrt cOLumnS packEd wiTH SilIca GEL witH BondeD pHenYlSulFoniC Acid grOUpS wHOSe HyDroGeN atoMS have been EXchanged for SilveR iONS) the rolE of silVer lies In its aBility tO fORM coMplexes wiTh unSAtuRaTed comPoUnDs.