So keeping this in mind, here is an example of the former example (TACG -> ATGC -> TACG).
In short, before starting, you need to know what you’re targeting and how you’re going to get there! So keeping this in mind, here is an example of the former example (TACG -> ATGC -> TACG). If the dideoxynucleotides labels are T = yellow, A = pink, C = dark blue, and G = light blue, you will end up with the short sequences primer-A, primer-AT, primer-ATG, and primer-ATGC. Once the fragments have been separated by electrophoresis, the laser will read the fragments in order of length (pink, yellow, light blue, and dark blue) and produce a chromatogram. The computer will convert the letters, so the final sequence is the correct TACG.