Standard endpoint PCR and RT-PCR are used to detect and amplify specific DNA sequences for further manipulation, including analysis by sequencing.
Real-time quantitative PCR, or qPCR, is a standard nucleic acid analysis tool used for detection and quantification of gene expression, and for detection of specific DNA sequences, including mutations. Standard endpoint PCR and RT-PCR are used to detect and amplify specific DNA sequences for further manipulation, including analysis by sequencing.