1. A computer-implemented method for creating a blood vessel map of a biological tissue, the method comprising the steps of: accessing image data corresponding to a multi-channel multiplexed image of a biological tissue fluorescent stained to manifest expression levels of a primary marker and at least one auxiliary marker, each marker marking blood vasculature; extracting features of blood vessels using the primary marker as an input to create an initial single channel segmentation of the blood vessels as a first input; extracting features of blood vessels using the at least one auxiliary marker to create auxiliary channel segmentations as a second input and applying multi-channel blood vessel enhancement to the first and second inputs; computing a probability map using the extracted features; tracing the blood vasculature by iteratively extending the centerlines of the initial single channel segmentation using statistical models and geometric rules; and generating a corresponding blood vessel map.