1. A method comprising: providing a database containing a plurality of reference Raman data sets wherein each said reference Raman data set has an associated known biological sample and an associated known metabolic state and at least one of the following associated with it: a corresponding reference Raman image, and a corresponding reference non-Raman image; using said reference Raman image, identifying one or more regions of interest of corresponding known biological sample, wherein said one or more regions of interest contain at least one of the following: an epithelium tissue, a stroma tissue, and a nuclei tissue irradiating a test biological sample with substantially monochromatic light to thereby generate scattered photons; collecting a test Raman data set based on said scattered photons; and comparing said test Raman data set to said plurality of reference Raman data sets using a chemometric technique; and based on said comparing, predicting a metabolic state of the test biological sample.