1. A graphical user interface (GUI) for setup and testing of machine vision systems comprising: an image frame pane in which recorded and real time images of an object acquired by an interconnected vision system are displayed as discrete frames; at least one box in which the user performs interface functions in connection with each of a plurality of different and discrete procedures to set up and run a machine vision job using the interconnected vision system; and a plurality of buttons, arranged in a sequential order across a portion of the GUI, the buttons defining each of the plurality of different and discrete procedures, the sequential order corresponding to a predetermined order in which the procedures occur to set up and run a machine vision job.