1. A method of evaluating the effect of interactions between distinct cell types, the method comprising: (a) providing a first cell culture of a first cell type and a second cell culture of a second cell type in a microenvironment in which the cells of the first and second cell cultures share a common medium, and wherein the first and second cell types interact in the common medium; (b) imaging the first and second cell types after exposure to an agent or stimulus; and (c) quantitatively evaluating one or more images obtained in (b) to identify any effects of the agent on quantitative representations of the phenotypes of the cells in the first and second cell cultures, which effects are mediated by interactions between the first and second cell cultures.