1. A computer-implemented method of analyzing fibers and branching structures of objects, the computer-implemented method comprising: obtaining an image of an object utilizing an imaging device, the object comprising fibers and/or branching structures; obtaining initial tracing seeds using adaptive thresholding method; identifying tracing seeds candidates by employing local Principal Component Analysis (PCA) calculation; removing the seeds having low score from the initial calculation; tracing a single fiber segment utilizing a fiber detection and a fiber tracing modules; assembling individual fiber segments utilizing a connectivity module; and forming and analyzing final fiber structures.