1. A method for transport and hybridization of DNA in an active electronic system comprising the steps of: providing a low conductivity, zwitterionic buffer on said device, electrophoretically transporting said nucleic acid towards a microlocation, applying current and voltage to the microlocation to effect transportation, whereby the local pH above the microlocation is below the pH of the buffer at its isoelectric point, whereby hybridization between the nucleic acid and a probe located at the microlocation is enhanced.