1. A method for determining the quality of a segmentation marker stained biological specimen, comprising: Acquiring separate image data of an unstained form of a biological specimen, the biological specimen stained with a nuclei marker and the biological specimen stained with a segmentation marker; Determining a correlation map (Cr) from the separate image data of the unstained form of the biological specimen, the biological specimen stained with the nuclei marker and the biological specimen stained with a segmentation marker; Determining a ridgeness map (Pr) from the image data of the biological specimen stained with the segmentation marker; and Determining a staining quality score from the correlation map (Cr) and the ridgeness map (Pr).