1. A computerized spatial-temporal regulation method for more accurate spatial-temporal model estimation in quantitative microscopy data analysis for detecting puncta related phenotypes comprising the steps of: a) Inputting a spatial temporal sequence containing an object confidence mask from microscopy time-lapse studies of subcellular components; b) Using the spatial temporal sequence to generate a temporal confidence sequence from variation of adjacent frame temporal intensity and integrating with the object confidence mask to obtain a weight sequence output; c) Performing weighted fitting using the spatial temporal sequence and the weight sequence to obtain at least one model parameter output.