1. A computer-implemented method for creating a blood vessel map of a biological tissue, the method comprising the steps of: accessing image data corresponding to multi-channel multiplexed image of a biological tissue fluorescent stained to manifest expression levels of a primary marker and at least one auxiliary marker each marker capable of marking blood vasculature; extracting features of blood vessels using the primary marker as an input to create a single channel segmentation of the blood vessels; extracting features of blood vessels using the at least one auxiliary marker to create auxiliary channels as a second input and apply multi-channel blood vessel enhancement; computing a probability map using the features; tracing the blood vasculature by iteratively extending the centerlines of the initial segmentation using statistical models and geometric rules; and generating a blood vessel map.