1. A computer-implemented method to automatically select time traces from a fluorescence experiment, comprising: capturing results of the fluorescence experiment in a moving image; localizing sources of fluorescence in the moving image; producing time traces of each fluorescent source by monitoring fluorescence intensity of said localized sources in the moving image over time; removing unuseful time traces from said produced time traces; and selecting useful time traces from said produced time traces based on one or more defined criteria, wherein the steps of capturing, localizing, producing, removing and selecting are performed by a processor.