1. A method of analysis of an array image including one or more luminous spots on a background, comprising: determining a shape and relative location of each spot on the array image; generating a binary map of pixels defining a boundary of each spot on the background; isolating each spot by an extraction operation using said binary map; examining each spot by a segmentation operation to identify pixels belonging to a same cluster according to a preestablished criterion; and for each spot, defining relative characteristic parameters and quality indexes; wherein said binary map is generated with a technique of morphological filtering comprising: filtering the array image with at least a morphological filter generating a corresponding “marker” image of the background; reconstructing said background by carrying out a reconstruction operation on said “marker” image to generate a corresponding reconstructed image of the background; and generating a filtered image of the luminosity of the background by performing a top-hat operation on said reconstructed background image and the array image; and performing a thresholding operation on said filtered image of the background luminosity.