Localization of lamin A at the endoplasmic reticulum upon the overexpression of SNX6. (A) Imaris 3D reconstruction of in vivo time-lapse confocal microscopy analysis of U2OS cells cotransfected with GFP-lamin A, HA-SNX6 (to promote extranuclear lamin A accumulation) and RFP-SEC61 (ER label). ER (red) and GFP-lamin A (green) and colocalization of both (yellow). U2OS cells cotransfected with RFP-Sec-61, GFP-Lamin A and HA-SNX6 were examined under a TCS SP5 confocal laser scanning unit attached to an inverted epifluorescence microscope (DMI6000) fitted with an HCX PL APO 63/NA 1.40-0.60 oil objective. Cells were maintained in DMEM (containing 10%FBS and 20 mM Hepes) in 35 mm dishes (MatTek) at 37°C in a 5% CO2 atmosphere.
González-Granado J, Navarro-Puche A, Molina-Sanchez P, Blanco-Berrocal M, Viana R, de Mora J, Andrés V
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275242/bin/pone.0115571.s005.mpg">S1 Video</a> from <cite style="font-style:normal">González-Granado J, Navarro-Puche A, Molina-Sanchez P, Blanco-Berrocal M, Viana R, de Mora J, Andrés V (2014). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275242">Sorting Nexin 6 Enhances Lamin A Synthesis and Incorporation into the Nuclear Envelope</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0115571">10.1371/journal.pone.0115571</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/25535984">25535984</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275242">4275242</a>.</cite>