The video shows the donor- (left, green), calculated FRET- (middle, colour coded), and the acceptor-images (right, yellow). DHT was added in frame 1 (time given in middle panel) at 10 nM. The donor images show a DHT induced increase in AR-ECFP translocation from the cytoplasm to the EYFP-SKIP positive nuclear speckles. This leads to a transient increase in FRET on the speckles, suggesting an increased number of AR/SKIP interactions. The FRET signal subsequently decreased, as the signal of EYFP-SKIP diminished.
Abankwa D, Millard S, Martel N, Choong C, Yang M, Butler L, Buchanan G, Tilley W, Ueki N, Hayman M, Leong G
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<cite style="font-style:normal">Abankwa D, Millard S, Martel N, Choong C, Yang M, Butler L, Buchanan G, Tilley W, Ueki N, Hayman M, Leong G (2013). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668167">Ski-interacting protein (SKIP) interacts with androgen receptor in the nucleus and modulates androgen-dependent transcription</a>". <i>BMC Biochemistry</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1186/1471-2091-14-10">10.1186/1471-2091-14-10</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/23566155">23566155</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668167">3668167</a>.</cite>