Animation showing sequences of (1) one tilt series used for the triple tilt series tomography, (2) X-Y slices through the triple tilt tomogram and (3) automated segmentation of six vesicles (gold = membrane; blue is proteinaceous material extending from the membrane into the cytoplasm). The small black dots at the top and bottom of the tomogram and in the tilt series are gold beads deposited on the top and bottom of the section that are used for fiducial alignment, beam and section distortion corrections in the 3D reconstruction process (see Materials and Methods). Data, metadata, segmentation and the full resolution movie for this tomogram can be downloaded from the Cell Centered Database (CCDB MPID:48720 PID:2010) as noted in the Materials and Methods section.
Boassa D, Nguyen P, Hu J, Ellisman M, Sosinsky G
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313697/bin/Video1.MOV">Supplemental Movie</a> from <cite style="font-style:normal">Boassa D, Nguyen P, Hu J, Ellisman M, Sosinsky G (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313697">Pannexin2 oligomers localize in the membranes of endosomal vesicles in mammalian cells while Pannexin1 channels traffic to the plasma membrane</a>". <i>Frontiers in Cellular Neuroscience</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.3389/fncel.2014.00468">10.3389/fncel.2014.00468</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313697">4313697</a>.</cite>