Cyclin A Can Drive APH+CHX Arrested Embryos into Mitosis. At the start of the movie, Pdi-GFP (green) and H2-RFP (red) expressing embryos were arrested with APH and CHX in cycle 11. At the break at 17:32 and 18:22 (frames 22 and 23), GST-CycA was then injected into the embryo. ER is seen gathering around the perispindle and poles while chromosomes condense and align at the metaphase plate. Frames were taken every 10 seconds. This video corresponds to Fig. 7B. Playback at 14 fps.
Bergman Z, Mclaurin J, Eritano A, Johnson B, Sims A, Riggs B
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331435/bin/pone.0117859.s012.avi">S6 Movie</a> from <cite style="font-style:normal">Bergman Z, Mclaurin J, Eritano A, Johnson B, Sims A, Riggs B (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331435">Spatial Reorganization of the Endoplasmic Reticulum during Mitosis Relies on Mitotic Kinase Cyclin A in the Early Drosophila Embryo</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0117859">10.1371/journal.pone.0117859</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/25689737">25689737</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331435">4331435</a>.</cite>