PTH-induces an elongated shape and increased motility in mature IDG-SW3 cells. IDG-SW3 cells were differentiated for 28 days and the mineral was imaged by adding 0.5μg/ml alizarin red to the culture media as a vital stain for calcium. The left panel shows control cultures treated with the PBS vehicle and the right panel shows cultures treated with 50nM PTH 1–34. Time lapse images were captured using a widefield epifluorescence live imaging microscope every 30 minutes. Note the dramatic elongation of the Dmp1-GFP positive cells in the PTH treated culture and the increased motility of these elongated cells compared to the control.
Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420268/bin/pone.0125731.s005.avi">S1 Video</a> from <cite style="font-style:normal">Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420268">Parathyroid Hormone Induces Bone Cell Motility and Loss of Mature Osteocyte Phenotype through L-Calcium Channel Dependent and Independent Mechanisms</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0125731">10.1371/journal.pone.0125731</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/25942444">25942444</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420268">4420268</a>.</cite>