Original figure legend: <i>Pointlike emitter optical response. From left to right: calculated x-y (above) and r-z (below) intensity distributions, in logarithmic scale, for a point like source imaged by means of wide-field, 2PE and confocal microscopy. Both 2PE and confocal shapes exhibit a better signal to noise ratio than widefield case. 2PE distribution is larger due the fact that a wavelength twice than in the wide-field and confocal case is responsible for the intensity distribution. Such intensity distributions are also known as point spread functions of the related microscopes. Optical conditions: excitation wavelengths are 488 nm and 900 nm for 1PE and 2PE, respectively; emission wavelength is 520 nm; numerical aperture is 1.3 for an oil immersion objective with oil refractive index value set at 1.515.</i>
Alberto Diaspro, Paolo Bianchini, Giuseppe Vicidomini, Mario Faretta, Paola Ramoino and Cesare Usai
CC BY 2.0
<p><i>Multi-photon excitation microscopy.</i> BioMedical Engineering OnLine, 2006, 5:36. <br>
DOI:10.1186/1475-925X-5-36. <br></p>
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