Live imaging of H2Av-RFP labeled nuclei in Cdk9 depleted embryos. Embryos derived from mothers with a histone H2Av-RFP transgene, TubGal4, and Cdk9 shmiRNA (Movie S2, Cdk9i) were dechorionated and imaged every 2 min. Images were assembled with ImageJ software into time-lapse movies. The nuclear fallout in the center of the Cdk9 embryo may be caused by the presence of the H2Av-RFP transgene, as it was also often observed in control embryos, whereas the loss of cells from the embryo posterior was specific to Cdk9 embryos.
Dahlberg O, Shilkova O, Tang M, Holmqvist P, Mannervik M
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334199/bin/pgen.1004971.s011.mp4">S2 Movie</a> from <cite style="font-style:normal">Dahlberg O, Shilkova O, Tang M, Holmqvist P, Mannervik M (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334199">P-TEFb, the Super Elongation Complex and Mediator Regulate a Subset of Non-paused Genes during Early Drosophila Embryo Development</a>". <i>PLOS Genetics</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pgen.1004971">10.1371/journal.pgen.1004971</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/25679530">25679530</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334199">4334199</a>.</cite>