===Aims/hypothesis===
<p>Low physical activity levels and sedentary behaviour are associated with obesity, insulin resistance and type 2 diabetes. We investigated the effects of a short-term reduction in physical activity with increased sedentary behaviour on metabolic profiles and body composition, comparing the effects in individuals with first-degree relatives with type 2 diabetes (FDR+ve) vs those without (FDR−ve).
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<h3><span class="mw-headline" id="Methods">Methods</span></h3>
<p>Forty-five habitually active participants (16 FDR+ve [10 female, 6 male] and 29 FDR−ve [18 female, 11 male]; age 36 ± 14 years) were assessed at baseline, after 14 days of step reduction and 14 days after resuming normal activity. We determined physical activity (using a SenseWear armband), cardiorespiratory fitness (V˙O2peak), body composition (dual-energy x-ray absorptiometry/magnetic resonance spectroscopy) and multi-organ insulin sensitivity (OGTT) at each time point. Statistical analysis was performed using a two-factor between-groups ANCOVA, with data presented as mean ± SD or (95% CI).
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<h3><span class="mw-headline" id="Results">Results</span></h3>
<p>There were no significant between-group differences in physical activity either at baseline or following step reduction. During the step-reduction phase, average daily step count decreased by 10,285 steps (95% CI 9389, 11,182; <i>p</i> &lt; 0.001), a reduction of 81 ± 8%, increasing sedentary time by 223 min/day (151, 295; <i>p</i> &lt; 0.001). Pooling data from both groups, following step reduction there was a significant decrease in whole-body insulin sensitivity (Matsuda index) (<i>p</i> &lt; 0.001), muscle insulin sensitivity index (<i>p</i> &lt; 0.001), cardiorespiratory fitness (<i>p</i> = 0.002) and lower limb lean mass (<i>p</i> = 0.004). Further, there was a significant increase in total body fat (<i>p</i> &lt; 0.001), liver fat (<i>p</i> = 0.001) and LDL-cholesterol (<i>p</i> = 0.013), with a borderline significant increase in NEFA AUC during the OGTT (<i>p</i> = 0.050). Four significant between-group differences were apparent: following step reduction, FDR+ve participants accumulated 1.5% more android fat (0.4, 2.6; <i>p</i> = 0.008) and increased triacylglycerol by 0.3 mmol/l (0.1, 0.6; <i>p</i> = 0.044). After resuming normal activity, FDR+ve participants engaged in lower amounts of vigorous activity (<i>p</i> = 0.006) and had lower muscle insulin sensitivity (<i>p</i> = 0.023). All other changes were reversed with no significant between-group differences.
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<h3><span id="Conclusions.2Finterpretation"></span><span class="mw-headline" id="Conclusions/interpretation">Conclusions/interpretation</span></h3>
A short-term reduction in physical activity with increased sedentary behaviour leads to a reversible reduction in multi-organ insulin sensitivity and cardiorespiratory fitness, with concomitant increases in central and liver fat and dyslipidaemia. The effects are broadly similar in FDR+ve and FDR−ve individuals. Public health recommendations promoting physical activity should incorporate advice to avoid periods of sedentary behaviour.
Kelly A. Bowden Davies, Victoria S. Sprung, Juliette A. Norman, Andrew Thompson, Katie L. Mitchell, Jason C. G. Halford, Jo A. Harrold, John P. H. Wilding, Graham J. Kemp &amp; Daniel J. Cuthbertson
CC BY 4.0
<a rel="nofollow" class="external free" href="https://link.springer.com/article/10.1007/s00125-018-4603-5">https://link.springer.com/article/10.1007/s00125-018-4603-5</a>