In vivo shuttling of lamin A to the nucleus. Time-lapse analysis of U2OS cells cotransfected with GFP-lamin A and HA-SNX6 to enhance GFP-lamin A extranuclear accumulation. Over a period of 8 hours, the extranuclear GFP-Lamin A progressively incorporated into the nucleus of the transfected cell. U2OS cells cotransfected with GFP-Lamin A and HA-SNX6 were examined under a Nikon ECLIPSE Ti time-lapse inverted microscope fitted with an 40× air objective (NA 0.6) using filters for GFP Cells were maintained in DMEM (containing 10%FBS and 20 mM Hepes) in 35 mm dishes (MatTek) at 37°C in a 5% CO2 atmosphere.
González-Granado J, Navarro-Puche A, Molina-Sanchez P, Blanco-Berrocal M, Viana R, de Mora J, Andrés V
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275242/bin/pone.0115571.s006.avi">S2 Video</a> from <cite style="font-style:normal">González-Granado J, Navarro-Puche A, Molina-Sanchez P, Blanco-Berrocal M, Viana R, de Mora J, Andrés V (2014). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275242">Sorting Nexin 6 Enhances Lamin A Synthesis and Incorporation into the Nuclear Envelope</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0115571">10.1371/journal.pone.0115571</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/25535984">25535984</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275242">4275242</a>.</cite>