Diltiazem blocks the elongated shape and increased motility in IDG-SW3 cells in response to PTH. IDG-SW3 cells were differentiated for 21 days and then treated with 50nM PTH 1–34 (left panel) or PTH + 100μM Diltiazem Hydrochloride (right panel). Time lapse images were captured using a widefield epifluorescence live imaging microscope every 30 minutes. Note the reproducible elongation of Dmp1-GFP positive cells and increase in motility with PTH treatment, which is blocked in the presence of diltiazem.
Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420268/bin/pone.0125731.s007.avi">S3 Video</a> from <cite style="font-style:normal">Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420268">Parathyroid Hormone Induces Bone Cell Motility and Loss of Mature Osteocyte Phenotype through L-Calcium Channel Dependent and Independent Mechanisms</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0125731">10.1371/journal.pone.0125731</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/25942444">25942444</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420268">4420268</a>.</cite>