Transient transfection with pCrkI/R38K is sufficient to cause cell death in HeLa cells that is apoptotic in nature. HeLa cells were transiently transfected with either pCrkI-GFP or the SH2 DN pCrkI/R38K-GFP expression vector or pCrkI/R38K-GFP pre-incubated with ZVAD, a broad pan-caspase inhibitor for 2h prior to transfection. Video images were captured every 15 min at 200× magnification. Cellular uptake of propidium iodide, an impermeant nuclear dye, identifies dying cells (red). Note that ZVAD significantly inhibits cell death of transfected cells.
Wood S, Goldufsky J, Shafikhani S
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447348/bin/ppat.1004934.s013.mov">S4 Movie</a> from <cite style="font-style:normal">Wood S, Goldufsky J, Shafikhani S (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447348">Pseudomonas aeruginosa ExoT Induces Atypical Anoikis Apoptosis in Target Host Cells by Transforming Crk Adaptor Protein into a Cytotoxin</a>". <i>PLOS Pathogens</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.ppat.1004934">10.1371/journal.ppat.1004934</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26020630">26020630</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447348">4447348</a>.</cite>