Dual-color single molecule imaging of Cy3-SNAP-EGFR and Alexa488-SNAP-EGFR in an unstimulated cell, and after 10 minutes stimulation with 16 nM EGF. Scale bar is 5 μm. Note the strong bleaching in the Alexa488 channel, which causes a loss of the background signal, and leads to an increase in the contrast with clustered Alexa488-SNAP-EGFR. This gives the visual impression of an increased signal in aggregates compared with the Cy3 channel, but does not indicate increased clustering.
Ibach J, Radon Y, Gelléri M, Sonntag M, Brunsveld L, Bastiaens P, Verveer P
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648588/bin/pone.0143162.s016.avi">S3 Movie</a> from <cite style="font-style:normal">Ibach J, Radon Y, Gelléri M, Sonntag M, Brunsveld L, Bastiaens P, Verveer P (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648588">Single Particle Tracking Reveals that EGFR Signaling Activity Is Amplified in Clathrin-Coated Pits</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0143162">10.1371/journal.pone.0143162</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26575183">26575183</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648588">4648588</a>.</cite>