Lytic granules tracking in live cell imaging microscopy. Target cells were pre-labeled with DiD membrane dye (red) and co-incubated with NK92.p46-IRES-GFP (green) pre-loaded with LysoTracker DND99 (gray) on a δT dish at 37°C. Time lapse images of random fields were recorded over 30–90 min at frame per 10 s rate. Granules polarization time was determined by minimal distance to the immune synapse as previously described (Mentlik MBOC 2010). Representative composite video shows time from initial interaction at 7 frames/s rate.
Hadad U, Thauland T, Martinez O, Butte M, Porgador A, Krams S
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585260/bin/Video_1.MP4">Video S1</a> from <cite style="font-style:normal">Hadad U, Thauland T, Martinez O, Butte M, Porgador A, Krams S (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585260">NKp46 Clusters at the Immune Synapse and Regulates NK Cell Polarization</a>". <i>Frontiers in Immunology</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.3389/fimmu.2015.00495">10.3389/fimmu.2015.00495</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26441997">26441997</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585260">4585260</a>.</cite>