U2OS cell competition is mediated by short-range cell interactions. Time-lapse microscospy video of R1:YFP spot co-cultures over 72 hours (1 frame = 15 min). Note that YFP (green nuclei) are progressively eliminated from the R1 (red nuclei) spot; however, the YFP cells outside the spot are not eliminated. Also note that in most cases death of YFP cells in the R1 spot is not preceded by cell detachment.
Penzo-Méndez A, Chen Y, Li J, Witze E, Stanger B
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511643/bin/pone.0132437.s012.mp4">S1 File</a> from <cite style="font-style:normal">Penzo-Méndez A, Chen Y, Li J, Witze E, Stanger B (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511643">Spontaneous Cell Competition in Immortalized Mammalian Cell Lines</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0132437">10.1371/journal.pone.0132437</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26200654">26200654</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511643">4511643</a>.</cite>