Original figure legend: <i>Multiple fluorescence 2PE imaging. 2PE multiple fluorescence image from a 16 μm cryostat section of mouse intestine stained with a combination of fluorescent stains (F-24631, Molecular Probes). Alexa Fluor 350 wheat germ agglutinin, a blue-fluorescent lectin, was used to stain the mucus of goblet cells. The filamentous actin prevalent in the brush border was stained with red-fluorescent Alexa Flu or 568 phalloidin. Finally, the nuclei were stained with SYTOX ® Green nucleic acid stain. Imaging has been performed at 780 nm, 100 x 1.4 NA Leica objective, using a Chameleon XR ultrafast Ti-Sapphire laser (Coherent Inc., USA) coupled at LAMBS-MicroScoBio with a Spectral Confocal Laser Scanning Microscope, Leica SP2-AOBS.</i>
Alberto Diaspro, Paolo Bianchini, Giuseppe Vicidomini, Mario Faretta, Paola Ramoino and Cesare Usai
CC BY 2.0
<p><i>Multi-photon excitation microscopy.</i> BioMedical Engineering OnLine, 2006, 5:36. <br>
DOI:10.1186/1475-925X-5-36. <br></p>
<a rel="nofollow" class="external free" href="http://www.biomedical-engineering-online.com/content/5/1/36">http://www.biomedical-engineering-online.com/content/5/1/36</a>