The dynamic processes of touch neuron necrosis and the exposure of PS on the surface of necrotic neurons monitored by time-lapse microscopy. Related to Fig 1E. Time-lapse recording was performed in a mec-4 (e1611dm) embryo, starting at ~460 min post-1st cleavage. The changes of PLML and PLMR, two touch neurons in the tail, were followed using the following three reporters: (1) Pmec-7 GFP, for the detecting specification of the touch neurons, (2) DIC microscopy, for monitoring the morphological changes throughout the necrosis process, and (3) MFG-E8::mCherry, for detecting PS on the surface of necrotic and apoptotic cells. At each time point, 40 slices of Z-sections were collected, with an interval of 0.5 μm. Arrowheads indicate both living and necrotic touch neurons. Arrows indicate apoptotic cells.
Li Z, Venegas V, Nagaoka Y, Morino E, Raghavan P, Audhya A, Nakanishi Y, Zhou Z
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464654/bin/pgen.1005285.s009.mov">S1 Movie</a> from <cite style="font-style:normal">Li Z, Venegas V, Nagaoka Y, Morino E, Raghavan P, Audhya A, Nakanishi Y, Zhou Z (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464654">Necrotic Cells Actively Attract Phagocytes through the Collaborative Action of Two Distinct PS-Exposure Mechanisms</a>". <i>PLOS Genetics</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pgen.1005285">10.1371/journal.pgen.1005285</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26061275">26061275</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464654">4464654</a>.</cite>