Multi-mode imaging of STIM/Orai relocalization following depletion of ER Ca 2+  stores. Images in each panel of the video show EGFP-tagged Orai visualized by shadowless TIRF excitation at 488 nm (top left); mCherry-tagged STIM visualized by TIRF excitation at 532 nm (top right); mCherry-tagged STIM visualized by skimming plane (SP) excitation at 532 nm (bottom left); and a pseudocolored overlay (Orai in green, TIRF-STIM in red, SP-STIM in blue) of these three images. The imaging modes were alternated with 100 ms exposures in each mode. Thapsigargin was applied shortly after the beginning of the record to deplete ER Ca2+ and induce STIM and Orai relocalization. Each panel is 81 x 61 μm.
Ellefsen K, Dynes J, Parker I
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550233/bin/pone.0136055.s004.mp4">S2 Movie</a> from <cite style="font-style:normal">Ellefsen K, Dynes J, Parker I (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550233">Spinning-Spot Shadowless TIRF Microscopy</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0136055">10.1371/journal.pone.0136055</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26308212">26308212</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550233">4550233</a>.</cite>