Video going through all images from the FIB/SEM tomography image stack (in the x-z and y-z planes), before the generation of surfaces from the stack is illustrated. Clearly resolved membranes were used to determine boundaries of the nucleus (blue), a vesicle (yellow) and bacteria (red).
Beckwith M, Beckwith K, Sikorski P, Skogaker N, Flo T, Halaas Ø
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583302/bin/pone.0134644.s005.mp4">S1 Video</a> from <cite style="font-style:normal">Beckwith M, Beckwith K, Sikorski P, Skogaker N, Flo T, Halaas Ø (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583302">Seeing a Mycobacterium-Infected Cell in Nanoscale 3D: Correlative Imaging by Light Microscopy and FIB/SEM Tomography</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0134644">10.1371/journal.pone.0134644</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26406896">26406896</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583302">4583302</a>.</cite>