PTH-induces an elongated shape and increased motility in primary calvarial cells, which is not blocked by deletion of  E11/gp38 . Primary osteoblasts were isolated from Dmp1-GFP transgenic mice (left panel) and Dmp1-GFP transgenic mice on an E11/gp38-deficient background (right panel). The E11-deficient mice were homozygous for the E11fl/fl allele, which functions as a hypomorph, resulting in negligible expression of E11/gp38. The cells were differentiated for 21 days and then both cultures were treated with 50nM PTH 1–34. Time lapse images were captured using a widefield epifluorescence live imaging microscope every 30 minutes. Note that both the Dmp1-GFP control primary cells and the Dmp1-GFP/E11-deficient primary cells show similar cell elongation and increased motility in response to PTH, suggesting that the lack of E11/gp38 does not block the PTH induced response.
Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420268/bin/pone.0125731.s006.avi">S2 Video</a> from <cite style="font-style:normal">Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420268">Parathyroid Hormone Induces Bone Cell Motility and Loss of Mature Osteocyte Phenotype through L-Calcium Channel Dependent and Independent Mechanisms</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0125731">10.1371/journal.pone.0125731</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/25942444">25942444</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420268">4420268</a>.</cite>