Time course of induction of NirA-GFP nuclear localisation. Strain gpdAp-nirA-GFP was grown directly on microscope coverslips for 6 h on a non-inducing nitrogen source (3 mM arginine) and subsequently exposed to 10 mM nitrate. Pictures were taken in the laser scanning microscope (scale bar = 10 μm) immediately before addition of nitrate (0 sec) and 20, 40, 60, 80 and 100 seconds after induction.
Gallmetzer A, Silvestrini L, Schinko T, Gesslbauer B, Hortschansky P, Dattenböck C, Muro-Pastor M, Kungl A, Brakhage A, Scazzocchio C, Strauss J
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488483/bin/pgen.1005297.s011.avi">S1 Video</a> from <cite style="font-style:normal">Gallmetzer A, Silvestrini L, Schinko T, Gesslbauer B, Hortschansky P, Dattenböck C, Muro-Pastor M, Kungl A, Brakhage A, Scazzocchio C, Strauss J (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488483">Reversible Oxidation of a Conserved Methionine in the Nuclear Export Sequence Determines Subcellular Distribution and Activity of the Fungal Nitrate Regulator NirA</a>". <i>PLOS Genetics</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pgen.1005297">10.1371/journal.pgen.1005297</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26132230">26132230</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488483">4488483</a>.</cite>