Time-lapse video of meiosis I in oocyte expressing CPAR-1::GFP. The time-lapse video captures the metaphase-to-anaphase transition in meiosis I, starting at prometaphase I and ending at anaphase I in a CPAR-1::GFP (OD145) expressing oocyte. GFP signal is retained on chromatin after the metaphase-to-anaphase transition. The video was processed as a 5 z-stack maximum projection, 8 μm (2 μm spacing) thick. Frames were captured at 20-second intervals and replayed at 7 frames per second (140x real-time). The embryos are oriented anterior left. Chromosomes on the left will form the first polar body and chromosomes on the right will continue into meiosis II. The scale of the video is 13.1 x 13.1 μm.
Monen J, Hattersley N, Muroyama A, Stevens D, Oegema K, Desai A
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412405/bin/pone.0125382.s003.mov">S3 Movie</a> from <cite style="font-style:normal">Monen J, Hattersley N, Muroyama A, Stevens D, Oegema K, Desai A (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412405">Separase Cleaves the N-Tail of the CENP-A Related Protein CPAR-1 at the Meiosis I Metaphase-Anaphase Transition in C. elegans</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0125382">10.1371/journal.pone.0125382</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/25919583">25919583</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412405">4412405</a>.</cite>