The SH3 domain of CrkI must be functional for CrkI to mediate ExoT cytotoxicity. Crk-/- cells were transfected with the pCrkI/R38K,W170K-GFP vector on consecutive days to increase transfection efficiency. 24 hr after final transfection, transfected cells were infected with PA103ΔexoU (ΔU), PA103∆exoU/exoT(R149K) (∆U/T(G-A+)), or the T3SS mutant PA103 pscJ::Tn5 (pscJ) at MOI ~10. Video images were captured every 15 min at 200× magnification. Cellular uptake of propidium iodide (PI), an impermeant nuclear dye, identifies dying cells (red). For clarity, phase panels were excluded from the movie. Note that transfected cells (green) do not pick up PI
Wood S, Goldufsky J, Shafikhani S
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447348/bin/ppat.1004934.s017.mp4">S8 Movie</a> from <cite style="font-style:normal">Wood S, Goldufsky J, Shafikhani S (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447348">Pseudomonas aeruginosa ExoT Induces Atypical Anoikis Apoptosis in Target Host Cells by Transforming Crk Adaptor Protein into a Cytotoxin</a>". <i>PLOS Pathogens</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.ppat.1004934">10.1371/journal.ppat.1004934</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26020630">26020630</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447348">4447348</a>.</cite>