Transient transfection with pCrkI/R38K is sufficient to cause cytotoxicity in Crk -/-  cells. Crk-/- cells were transfected with either pIRES2-GFP empty vector, pCrkI-GFP, pCrkI/R38K-GFP, or pCrkI/R38K,W170K-GFP. Video images were captured every 15 min at 200× magnification. Cellular uptake of propidium iodide, an impermeant nuclear dye, identifies dying cells (red). For clarity, phase panels were excluded from the movie. Note that only pCrkI/R38K transfected cells pick up PI.
Wood S, Goldufsky J, Shafikhani S
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447348/bin/ppat.1004934.s016.mov">S7 Movie</a> from <cite style="font-style:normal">Wood S, Goldufsky J, Shafikhani S (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447348">Pseudomonas aeruginosa ExoT Induces Atypical Anoikis Apoptosis in Target Host Cells by Transforming Crk Adaptor Protein into a Cytotoxin</a>". <i>PLOS Pathogens</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.ppat.1004934">10.1371/journal.ppat.1004934</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26020630">26020630</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447348">4447348</a>.</cite>