Transendothelial migration of BMDN is regulated by SIRPα signaling. BMDN were flown over a monolayer of endothelial cells for 5 min, allowed to rest on the endothelial cells for other 5 min and then subjected to flow conditions for 20 min. Transmigrating events were recognize as phase-contrast negative BMDN.
Alvarez-Zarate J, Matlung H, Matozaki T, Kuijpers T, Maridonneau-Parini I, van den Berg T
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461249/bin/pone.0127178.s003.mpg">S1 Video</a> from <cite style="font-style:normal">Alvarez-Zarate J, Matlung H, Matozaki T, Kuijpers T, Maridonneau-Parini I, van den Berg T (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461249">Regulation of Phagocyte Migration by Signal Regulatory Protein-Alpha Signaling</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0127178">10.1371/journal.pone.0127178</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26057870">26057870</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461249">4461249</a>.</cite>