The effect of mycolactone on the expression of cytosolic GFP. HeLa cells were stably transfected with a plasmid encoding GFP alone (expressed in the cytosol). Cells were exposed to 125ng/ml mycolactone for 21hrs and fluorescence was captured by time-lapse microscopy at 20min intervals using a Nikon A1 confocal laser scanning unit attached to an Eclipse Ti microscope. Mycolactone was added to the wells before assembling the humidified chamber and setting up the experiment, therefore the first time point is approximately 1hr after reagent addition. One whole microscope field out of three per condition is shown. Videos were compressed in ImageJ and consist of 64 frames apiece and run at 6fps
Ogbechi J, Ruf M, Hall B, Bodman-Smith K, Vogel M, Wu H, Stainer A, Esmon C, Ahnström J, Pluschke G, Simmonds R
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504485/bin/ppat.1005011.s004.avi">S4 Video</a> from <cite style="font-style:normal">Ogbechi J, Ruf M, Hall B, Bodman-Smith K, Vogel M, Wu H, Stainer A, Esmon C, Ahnström J, Pluschke G, Simmonds R (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504485">Mycolactone-Dependent Depletion of Endothelial Cell Thrombomodulin Is Strongly Associated with Fibrin Deposition in Buruli Ulcer Lesions</a>". <i>PLOS Pathogens</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.ppat.1005011">10.1371/journal.ppat.1005011</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26181660">26181660</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504485">4504485</a>.</cite>