W/O droplet electroporation was performed under a DC electric field intensity of approximately 2.5 kV/cm with electrodes for disposable 96-well plates. The droplets of PBS buffer were colored by trypan blue without cells and plasmid DNA.
Kurita H, Takahashi S, Asada A, Matsuo M, Kishikawa K, Mizuno A, Numano R
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674099/bin/pone.0144254.s006.avi">S1 Movie</a> from <cite style="font-style:normal">Kurita H, Takahashi S, Asada A, Matsuo M, Kishikawa K, Mizuno A, Numano R (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674099">Novel Parallelized Electroporation by Electrostatic Manipulation of a Water-in-Oil Droplet as a Microreactor</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0144254">10.1371/journal.pone.0144254</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26649904">26649904</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674099">4674099</a>.</cite>