Original figure legend: <i>Optical sectioning scheme. A three-dimensional sample can be sketched as a series of optical slices. Let's call slice j the one containing the geometrical focus of the objective and refer to the adjacent planes as k slices. The sample contains a three-dimensional distribution of fluorescently labelled molecules whose intensity distribution is I, slice by slice. The thickness of each optical slice is approximately one half of the axial resolution, say ≈λ/2.</i>
Alberto Diaspro, Paolo Bianchini, Giuseppe Vicidomini, Mario Faretta, Paola Ramoino and Cesare Usai
CC BY 2.0
<p><i>Multi-photon excitation microscopy.</i> BioMedical Engineering OnLine, 2006, 5:36. <br>
DOI:10.1186/1475-925X-5-36. <br></p>
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