Simultaneous imaging of fluorescent beads in suspension by TIRF and wide-field excitation. Aqueous suspension of fluorescent microspheres (0.5 μm diameter) imaged by alternating shadowless TIRF and widefield excitation. Images were acquired by alternating 5 ms exposures in each mode. The montage shows de-interlaced images (widefield, left; TIRF, right) after averaging 2 sequential frames. Three stationary microspheres had adhered to the coverglass, and others can be seen undergoing Brownian motion in the water. Image is 51 x 51 μm.
Ellefsen K, Dynes J, Parker I
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550233/bin/pone.0136055.s003.mp4">S1 Movie</a> from <cite style="font-style:normal">Ellefsen K, Dynes J, Parker I (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550233">Spinning-Spot Shadowless TIRF Microscopy</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0136055">10.1371/journal.pone.0136055</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26308212">26308212</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550233">4550233</a>.</cite>