Intermittant iron supply with single cell events. After 3.7 hours, the perfusion medium was switched to without carbon (CGXII—PCA). The limitation of carbon arrested the growth of C. glutamiucum cells until full media supply was returned after 15.2 hours.
Krämer C, Singh A, Helfrich S, Grünberger A, Wiechert W, Nöh K, Kohlheyer D
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<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625966/bin/pone.0141768.s007.mp4">S7 Video</a> from <cite style="font-style:normal">Krämer C, Singh A, Helfrich S, Grünberger A, Wiechert W, Nöh K, Kohlheyer D (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625966">Non-Invasive Microbial Metabolic Activity Sensing at Single Cell Level by Perfusion of Calcein Acetoxymethyl Ester</a>". <i>PLOS ONE</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0141768">10.1371/journal.pone.0141768</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26513257">26513257</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625966">4625966</a>.</cite>