Cell tracking of leukocytes co-cultured with EIT group of organ of Corti and lateral wall tissues. Four cochlear tissue explants were used, with a total of 3 independent replicates. Sequential images of each group were taken every 15 s for 20 min with a 20× lens in a Zeiss LSM 700/ confocal upright microscope. ImageJ with Trackmate plugin was used to make the movies. The detected cells are included in a magenta circle and the line color codes are in function of the velocity for that particular cell. A rainbow color code displays dark blue lines for slow moving cells and red for fast moving cells (3.2 μm/s). Scale bar = 100 μm.
Bas E, Goncalves S, Adams M, Dinh C, Bas J, Van De Water T, Eshraghi A
CC BY 4.0
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532929/bin/Video3.AVI">Supplemental Movie 3</a> from <cite style="font-style:normal">Bas E, Goncalves S, Adams M, Dinh C, Bas J, Van De Water T, Eshraghi A (2015). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532929">Spiral ganglion cells and macrophages initiate neuro-inflammation and scarring following cochlear implantation</a>". <i>Frontiers in Cellular Neuroscience</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.3389/fncel.2015.00303">10.3389/fncel.2015.00303</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/26321909">26321909</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532929">4532929</a>.</cite>