abstract: STUDY OBJECTIVE: Opportunistic brief in- @entity0 @entity2 ( @entity2 ) interventions can be effective at reducing hazardous @entity1 use in young adult drinkers, but require resources frequently unavailable. Mobile telephone text messaging ( @entity3 [ @entity3 ]) could sustainably deliver behavioral support to young adult @entity0 , but efficacy remains unknown. We report 3-month outcome data of a randomized controlled trial testing a novel @entity3 -delivered intervention in hazardous-drinking young adults. METHODS: We randomized 765 young adult @entity2 @entity0 who screened positive for past hazardous @entity1 use to one of 3 groups: @entity3 assessments+feedback (SA+F) intervention who were asked to respond to drinking-related queries and received real-time feedback through @entity3 each Thursday and Sunday for 12 weeks (n=384), @entity3 assessments (SA) who were asked to respond to @entity1 consumption queries each Sunday but did not receive any feedback (N=196), and a control group who did not participate in any @entity3 (n=185). Primary outcomes were self-reported number of binge drinking days and number of drinks per drinking day in the past 30 days, collected by Web-based timeline follow-back method and analyzed with regression models. Secondary outcomes were the proportion of @entity0 with weekend binge episodes and most drinks consumed per drinking occasion during 12 weekends, collected by @entity3 . RESULTS: With Web-based data, there were decreases in the number of self-reported binge drinking days from baseline to 3 months in the SA+F group (-0.51 [95% confidence interval {CI} -0.10 to -0.95]), whereas there were increases in the SA group (0.90 [95% CI 0.23 to 1.6]) and the control group (0.41 [95% CI -0.20 to 1.0]). There were also decreases in the number of self-reported drinks per drinking day from baseline to 3 months in the SA+F group (-0.31 [95% CI -0.07 to -0.55]), whereas there were increases in the SA group (0.10 [95% CI -0.27 to 0.47]) and the control group (0.39 [95% CI 0.06 to 0.72]). With @entity3 data, there was a lower mean proportion of SA+F @entity0 reporting a weekend binge during 12 weeks (30.5% [95% CI 25% to 36%) compared with the SA @entity0 (47.7% [95% CI 40% to 56%]). There was also a lower mean drinks consumed per weekend during 12 weeks in the SA+F group (3.2 [95% CI 2.6 to 3.7]) compared to the SA group (4.8 [95% CI 4.0 to 5.6]). CONCLUSION: A text message intervention can produce small reductions in self-reported binge drinking and the number of drinks consumed per drinking day in hazardous-drinking young adults after @entity2 discharge.

title: A text message @entity1 intervention for young adult XXXX @entity0 : a randomized clinical trial.

answer: @entity2


abstract: STUDY OBJECTIVE: Opportunistic brief in- @entity0 @entity2 ( @entity2 ) interventions can be effective at reducing hazardous @entity1 use in young adult drinkers, but require resources frequently unavailable. Mobile telephone text messaging ( @entity3 [ @entity3 ]) could sustainably deliver behavioral support to young adult @entity0 , but efficacy remains unknown. We report 3-month outcome data of a randomized controlled trial testing a novel @entity3 -delivered intervention in hazardous-drinking young adults. METHODS: We randomized 765 young adult @entity2 @entity0 who screened positive for past hazardous @entity1 use to one of 3 groups: @entity3 assessments+feedback (SA+F) intervention who were asked to respond to drinking-related queries and received real-time feedback through @entity3 each Thursday and Sunday for 12 weeks (n=384), @entity3 assessments (SA) who were asked to respond to @entity1 consumption queries each Sunday but did not receive any feedback (N=196), and a control group who did not participate in any @entity3 (n=185). Primary outcomes were self-reported number of binge drinking days and number of drinks per drinking day in the past 30 days, collected by Web-based timeline follow-back method and analyzed with regression models. Secondary outcomes were the proportion of @entity0 with weekend binge episodes and most drinks consumed per drinking occasion during 12 weekends, collected by @entity3 . RESULTS: With Web-based data, there were decreases in the number of self-reported binge drinking days from baseline to 3 months in the SA+F group (-0.51 [95% confidence interval {CI} -0.10 to -0.95]), whereas there were increases in the SA group (0.90 [95% CI 0.23 to 1.6]) and the control group (0.41 [95% CI -0.20 to 1.0]). There were also decreases in the number of self-reported drinks per drinking day from baseline to 3 months in the SA+F group (-0.31 [95% CI -0.07 to -0.55]), whereas there were increases in the SA group (0.10 [95% CI -0.27 to 0.47]) and the control group (0.39 [95% CI 0.06 to 0.72]). With @entity3 data, there was a lower mean proportion of SA+F @entity0 reporting a weekend binge during 12 weeks (30.5% [95% CI 25% to 36%) compared with the SA @entity0 (47.7% [95% CI 40% to 56%]). There was also a lower mean drinks consumed per weekend during 12 weeks in the SA+F group (3.2 [95% CI 2.6 to 3.7]) compared to the SA group (4.8 [95% CI 4.0 to 5.6]). CONCLUSION: A text message intervention can produce small reductions in self-reported binge drinking and the number of drinks consumed per drinking day in hazardous-drinking young adults after @entity2 discharge.

title: A text message XXXX intervention for young adult @entity2 @entity0 : a randomized clinical trial.

answer: @entity1


abstract: STUDY OBJECTIVE: Opportunistic brief in- @entity0 @entity2 ( @entity2 ) interventions can be effective at reducing hazardous @entity1 use in young adult drinkers, but require resources frequently unavailable. Mobile telephone text messaging ( @entity3 [ @entity3 ]) could sustainably deliver behavioral support to young adult @entity0 , but efficacy remains unknown. We report 3-month outcome data of a randomized controlled trial testing a novel @entity3 -delivered intervention in hazardous-drinking young adults. METHODS: We randomized 765 young adult @entity2 @entity0 who screened positive for past hazardous @entity1 use to one of 3 groups: @entity3 assessments+feedback (SA+F) intervention who were asked to respond to drinking-related queries and received real-time feedback through @entity3 each Thursday and Sunday for 12 weeks (n=384), @entity3 assessments (SA) who were asked to respond to @entity1 consumption queries each Sunday but did not receive any feedback (N=196), and a control group who did not participate in any @entity3 (n=185). Primary outcomes were self-reported number of binge drinking days and number of drinks per drinking day in the past 30 days, collected by Web-based timeline follow-back method and analyzed with regression models. Secondary outcomes were the proportion of @entity0 with weekend binge episodes and most drinks consumed per drinking occasion during 12 weekends, collected by @entity3 . RESULTS: With Web-based data, there were decreases in the number of self-reported binge drinking days from baseline to 3 months in the SA+F group (-0.51 [95% confidence interval {CI} -0.10 to -0.95]), whereas there were increases in the SA group (0.90 [95% CI 0.23 to 1.6]) and the control group (0.41 [95% CI -0.20 to 1.0]). There were also decreases in the number of self-reported drinks per drinking day from baseline to 3 months in the SA+F group (-0.31 [95% CI -0.07 to -0.55]), whereas there were increases in the SA group (0.10 [95% CI -0.27 to 0.47]) and the control group (0.39 [95% CI 0.06 to 0.72]). With @entity3 data, there was a lower mean proportion of SA+F @entity0 reporting a weekend binge during 12 weeks (30.5% [95% CI 25% to 36%) compared with the SA @entity0 (47.7% [95% CI 40% to 56%]). There was also a lower mean drinks consumed per weekend during 12 weeks in the SA+F group (3.2 [95% CI 2.6 to 3.7]) compared to the SA group (4.8 [95% CI 4.0 to 5.6]). CONCLUSION: A text message intervention can produce small reductions in self-reported binge drinking and the number of drinks consumed per drinking day in hazardous-drinking young adults after @entity2 discharge.

title: A text message @entity1 intervention for young adult @entity2 XXXX : a randomized clinical trial.

answer: @entity0


abstract: SUMMARY Indoor transmission of @entity2 partly depends on the physico-chemical conditions in bedding material. Temperature, pH, aqueous @entity0 , moisture, occurrence and development of A. suum eggs were therefore compared in different areas (resting, intermediate and latrine) of two deep litter pens on an organic farm in four seasons. There was some variation, but mean @entity0 levels were generally very low (1  0-2  6  mm) and pH levels were moderate (8  04-8  88) in all three areas. Relatively, resting areas were characterized by overall moderate moisture (36%) and moderately high temperature (35  7    C) levels. The area contained few eggs (50  eggs  g-1 @entity1 ) of which 17% were viable, and though only 4% were larvated and 0  7% appeared infective, it was more than in the other areas. Intermediate areas had moderate moisture (43%) and high temperature (43  6    C) levels. There were many eggs (523  eggs  g-1 @entity1 ), but overall viability was very low (5%) and few eggs were larvated (0  004%) or even infective (0  002%). Latrines typically had high moisture (79%) and moderate temperature (30    C) levels. The concentration of eggs was very high (1444  egg  g-1 @entity1 ) and though 32% were viable, none had developed larval stages. The large majority of A. suum eggs appear to die and only few become infective while in the deep litter. However, a large fraction of eggs may remain viable for some time and could thus contaminate agricultural land and develop to infectivity, if the manure is not composted appropriately.

title: Development and survival of XXXX eggs in deep litter of pigs.

answer: @entity2


abstract: OBJECTIVES:: Midgut @entity5 with @entity4 is typical in newborns. We present our experience to underline the importance of suspecting @entity7 as cause of @entity3 also beyond infancy, particularly in relationship with @entity4 , and the relevance of ultrasonographic (US) signs in its diagnosis. MATERIALS AND METHODS:: 34 @entity0 (10M, 24 F; aged between 1 day and 12 years) diagnosed with @entity4 or @entity4 with @entity5 , surgically confirmed, over a period between 2006 and 2013 were retrospectively selected among all @entity0 referred to our institution for acute @entity2 . They all underwent US and color Doppler examination before surgery. The US examinations were performed with 6-10 Mhz microconvex and 7.5-10 Mhz linear transducer. The transducer was placed under xiphoid and, with axial projection, it was evaluated the presence of anatomical position reversed between superior mesenteric vein (SMV) and @entity6 ( @entity6 ) as sign of @entity4 , and the presence of "Whirpool Sign" ( @entity1 ) (wrapping of the SMV and the mesentery around the @entity6 ), as sign of @entity7 were evaluated. RESULTS:: In 27 of these 34 @entity0 , @entity7 was present; 7 @entity0 had @entity4 . In 2/7 (28%) @entity0 with @entity4 @entity6 and SMV were inverted. Among the @entity0 with @entity5 , 2 showed reversed vessels position and 22 @entity0 presented the Whirlpool sign in association with @entity6 /SMV inversion (22/27, 81%). CONCLUSIONS:: Midgut @entity5 with @entity4 can appear beyond the neonatal age group as demonstrated in our case. The @entity1 sign is sufficiently sensitive for its diagnosis and should be routinely researched at all ages of pediatric population. Anatomic inversion between SMV and @entity6 seems to be not enough sensitive in @entity4 diagnosis.

title: Ultrasonographic Diagnosis of XXXX With Malrotation in @entity0 : 7-Years Single Center Experience.

answer: @entity7


abstract: OBJECTIVES:: Midgut @entity5 with @entity4 is typical in newborns. We present our experience to underline the importance of suspecting @entity7 as cause of @entity3 also beyond infancy, particularly in relationship with @entity4 , and the relevance of ultrasonographic (US) signs in its diagnosis. MATERIALS AND METHODS:: 34 @entity0 (10M, 24 F; aged between 1 day and 12 years) diagnosed with @entity4 or @entity4 with @entity5 , surgically confirmed, over a period between 2006 and 2013 were retrospectively selected among all @entity0 referred to our institution for acute @entity2 . They all underwent US and color Doppler examination before surgery. The US examinations were performed with 6-10 Mhz microconvex and 7.5-10 Mhz linear transducer. The transducer was placed under xiphoid and, with axial projection, it was evaluated the presence of anatomical position reversed between superior mesenteric vein (SMV) and @entity6 ( @entity6 ) as sign of @entity4 , and the presence of "Whirpool Sign" ( @entity1 ) (wrapping of the SMV and the mesentery around the @entity6 ), as sign of @entity7 were evaluated. RESULTS:: In 27 of these 34 @entity0 , @entity7 was present; 7 @entity0 had @entity4 . In 2/7 (28%) @entity0 with @entity4 @entity6 and SMV were inverted. Among the @entity0 with @entity5 , 2 showed reversed vessels position and 22 @entity0 presented the Whirlpool sign in association with @entity6 /SMV inversion (22/27, 81%). CONCLUSIONS:: Midgut @entity5 with @entity4 can appear beyond the neonatal age group as demonstrated in our case. The @entity1 sign is sufficiently sensitive for its diagnosis and should be routinely researched at all ages of pediatric population. Anatomic inversion between SMV and @entity6 seems to be not enough sensitive in @entity4 diagnosis.

title: Ultrasonographic Diagnosis of @entity7 With Malrotation in XXXX : 7-Years Single Center Experience.

answer: @entity0


abstract: Background and Aim: Widespread multidrug-resistant @entity3 (MDR-AB) strains have limited therapeutic options for treating intensive care unit (ICU) @entity0 with MDR-AB infection in Iran. We aimed to evaluate MDR-AB diversity and antimicrobial susceptibility in Tehran (Iran) to address the need for feasible and effective control approaches against @entity6 . Methods: We used amplified fragment length polymorphism (AFLP) and minimum inhibitory concentration (MIC) determinations to compare genotypic diversity and susceptibility patterns of 100 MDR-AB isolates from ICU @entity0 in two medical centers in Tehran (Iran), from 2006 to 2011. Results: Within 5 years, drastic genotypic changes occurred among MDR-AB isolates, and resistance to antimicrobials increased 0-30%. In 2011, 6-100% of isolates were resistant to every agent tested. All isolates remained susceptible to either @entity1 or @entity2 , however, MIC50 concentrations against these agents increased. Novel international clone (IC) variants (not IC I-III types) comprised 36% MDR-AB isolates in 2011. Conclusions: The MDR-AB population in Tehran is rapidly changing toward growing resistance to various antimicrobials, including @entity4 and @entity5 . Although increasing resistance to last-resort antimicrobials is alarming, simultaneous susceptibility of all MDR-AB isolates to some conventional antibiotics highlights the merits of investigating their synergistic activity against extended-spectrum and pandrug resistant A. baumannii. Integrating the novel Iranian MDR-AB IC variants into epidemiologic clonal and susceptibility profile databases can help global efforts toward the control of MDR-AB pandemic.

title: Multidrug Resistance Among XXXX Isolates from Iran: Changes in Antimicrobial Susceptibility Patterns and Genotypic Profile.

answer: @entity3


abstract: BACKGROUND: Adipose-derived regenerative cells (ADRCs) are a promising source of autologous stem cells for regeneration and repair of damaged tissue. Herein, we investigated the therapeutic potential of ADRC sheets created by a magnetite tissue engineering technology (Mag-TE) for @entity4 . METHODS AND RESULTS: Adipose tissue was obtained from @entity2 ( @entity2 ) @entity0 and ADRCs were isolated. ADRCs incubated with magnetic nanoparticle-containing liposomes (MCLs) were cultured. MCL-labeled ADRCs were mixed with a diluted extracellular matrix (ECM) precursor, and a magnet was placed on the reverse side. Magnetized ADRCs formed multilayered cell sheets after a 24-h incubation. @entity2 @entity0 were subjected to @entity4 by permanent ligation of the left anterior descending artery. We then transplanted the constructed ADRC sheet or a cell-free collagen gel sheet, as a control, onto the infarcted myocardium using an Alnico magnet before skin closure. Cardiac parameters were measured by echocardiogram, and angiogenesis was determined by tissue capillary density. ADRC sheet-treated @entity0 showed significant improvements in systolic function, @entity6 wall thinning, and @entity8 after @entity4 . ADRC sheet implantation also promoted angiogenesis in both the @entity6 area and the border zone in @entity2 @entity0 after @entity4 . The angiogenic effects of ADRC sheets were attributed to an increased expression of @entity7 and @entity9 mRNA in @entity1 hearts. CONCLUSIONS: ADRC sheets created by this Mag-TE method protect the heart against @entity3 . Our ADRC sheets have the potential to be a novel regenerative strategy for @entity5 .

title: Multilayered adipose-derived regenerative cell sheets created by a novel magnetite tissue engineering method for XXXX .

answer: @entity4


abstract: OBJECTIVES: The purposes of this study were: (1) to assess the feasibility of optical coherence tomography (OCT) for detecting @entity3 ; and (2) to clarify the impact of @entity3 on coronary vessel behavior over time. BACKGROUND: Plaque @entity3 may be related to plaque vulnerability. METHODS: In an ex vivo study, a total of 55 coronary plaques from 31 @entity0 cadavers were examined by OCT. Plaque @entity3 was diagnosed based on the presence or absence of microchannels (MCs) by OCT. In an in vivo study, we explored 83 major coronary arteries from 42 @entity0 with @entity2 . A total of 56 coronary plaques were selected from non-culprit (non-stented) lesions with plaque burden >40% by intravascular ultrasound (IVUS). These plaques were classified into two groups based on the presence or absence of MC by OCT. RESULTS: In the ex vivo study, the sensitivity and specificity of OCT to detect @entity3 were 52% and 68%, respectively. In the in vivo study, MC was detected in 25 plaques (44.6%). High-density lipoprotein @entity1 was significantly lower in the MC group vs the non-MC group (42.7    7.8 mg/dL vs 51.6    14.4 mg/dL; P=.02). Percent change in lumen volume index (VI) in the MC group was significantly greater vs the non-MC group (P=.01). Percent change in lumen VI correlated significantly with percent change in external elastic membrane VI (P=.01). CONCLUSIONS: OCT was feasible for detecting @entity3 of coronary plaques. Serial OCT examinations revealed that coronary plaques with @entity3 showed greater luminal narrowing as a result of inadequate adaptive vessel remodeling.

title: Detection of Plaque Neovascularization by Optical Coherence Tomography: Ex Vivo Feasibility Study and In Vivo Observation in @entity0 With XXXX .

answer: @entity2


abstract: OBJECTIVES: The purposes of this study were: (1) to assess the feasibility of optical coherence tomography (OCT) for detecting @entity3 ; and (2) to clarify the impact of @entity3 on coronary vessel behavior over time. BACKGROUND: Plaque @entity3 may be related to plaque vulnerability. METHODS: In an ex vivo study, a total of 55 coronary plaques from 31 @entity0 cadavers were examined by OCT. Plaque @entity3 was diagnosed based on the presence or absence of microchannels (MCs) by OCT. In an in vivo study, we explored 83 major coronary arteries from 42 @entity0 with @entity2 . A total of 56 coronary plaques were selected from non-culprit (non-stented) lesions with plaque burden >40% by intravascular ultrasound (IVUS). These plaques were classified into two groups based on the presence or absence of MC by OCT. RESULTS: In the ex vivo study, the sensitivity and specificity of OCT to detect @entity3 were 52% and 68%, respectively. In the in vivo study, MC was detected in 25 plaques (44.6%). High-density lipoprotein @entity1 was significantly lower in the MC group vs the non-MC group (42.7    7.8 mg/dL vs 51.6    14.4 mg/dL; P=.02). Percent change in lumen volume index (VI) in the MC group was significantly greater vs the non-MC group (P=.01). Percent change in lumen VI correlated significantly with percent change in external elastic membrane VI (P=.01). CONCLUSIONS: OCT was feasible for detecting @entity3 of coronary plaques. Serial OCT examinations revealed that coronary plaques with @entity3 showed greater luminal narrowing as a result of inadequate adaptive vessel remodeling.

title: Detection of Plaque Neovascularization by Optical Coherence Tomography: Ex Vivo Feasibility Study and In Vivo Observation in XXXX With @entity2 .

answer: @entity0


abstract: OBJECTIVE: The association between biochemical markers and left ventricular ejection fraction in @entity0 with @entity3 was not completely studied. Our goal is to study the association between biochemical markers and @entity5 in @entity0 with ST-elevation acute @entity3 . METHODS: With an observational and prospective design we included @entity0 with less than 24h ST-elevation @entity3 . Leukocytes, @entity2 , B-type natriuretic peptide and T troponin were measured at admission, and @entity4 -phosphokinase and @entity4 -phosphokinase-MB were measured at admission and serially, and correlated with the ejection fraction estimated by echocardiography. RESULTS: A total of 108 @entity0 were included. The median left ventricular ejection fraction was 48% (interquartile range 41-57). Simple linear regression analysis showed that B-type natriuretic peptide (P=.005), peak @entity4 -phosphokinase-MB (P=.01), leukocyte count (P=.001) and @entity2 (P=.033) were inversely and significantly associated with the left ventricular ejection fraction. The other parameters showed no association. B-type natriuretic peptide (P=.01) and peak @entity4 -phosphokinase-MB (P=.02) were the only two variables significantly associated with the left ventricular ejection fraction in the multiple linear regression analysis. Both markers were significantly associated with a left ventricular ejection fraction < 50%, independently of other clinical variables. CONCLUSION: B-type natriuretic peptide and peak @entity4 -phosphokinase-MB showed significant association with left ventricular ejection fraction in the acute phase of ST elevation acute @entity3 . This association was independent of the presence of other biochemical markers and clinical variables related to @entity1 .

title: [Association between biochemical markers and XXXX in the ST-elevation acute @entity3 .]

answer: @entity5


abstract: OBJECTIVE: The association between biochemical markers and left ventricular ejection fraction in @entity0 with @entity3 was not completely studied. Our goal is to study the association between biochemical markers and @entity5 in @entity0 with ST-elevation acute @entity3 . METHODS: With an observational and prospective design we included @entity0 with less than 24h ST-elevation @entity3 . Leukocytes, @entity2 , B-type natriuretic peptide and T troponin were measured at admission, and @entity4 -phosphokinase and @entity4 -phosphokinase-MB were measured at admission and serially, and correlated with the ejection fraction estimated by echocardiography. RESULTS: A total of 108 @entity0 were included. The median left ventricular ejection fraction was 48% (interquartile range 41-57). Simple linear regression analysis showed that B-type natriuretic peptide (P=.005), peak @entity4 -phosphokinase-MB (P=.01), leukocyte count (P=.001) and @entity2 (P=.033) were inversely and significantly associated with the left ventricular ejection fraction. The other parameters showed no association. B-type natriuretic peptide (P=.01) and peak @entity4 -phosphokinase-MB (P=.02) were the only two variables significantly associated with the left ventricular ejection fraction in the multiple linear regression analysis. Both markers were significantly associated with a left ventricular ejection fraction < 50%, independently of other clinical variables. CONCLUSION: B-type natriuretic peptide and peak @entity4 -phosphokinase-MB showed significant association with left ventricular ejection fraction in the acute phase of ST elevation acute @entity3 . This association was independent of the presence of other biochemical markers and clinical variables related to @entity1 .

title: [Association between biochemical markers and @entity5 in the ST-elevation acute XXXX .]

answer: @entity3


abstract: OBJECTIVE: @entity2 ( @entity2 ) provides prompt airway control during emergency evacuation of @entity1 @entity0 . Physicians may be tasked to travel with paramedic ambulance crews to the scene of injury when @entity2 is more likely to be required. This study investigates whether there are any differences in the practice of @entity2 during emergency retrievals for @entity1 since the establishment of the regional @entity1 Network (MTN) in March 2012. METHODS: A retrospective observational study examined prehospital records for all @entity1 @entity0 from September 2010 to January 2015 at a @entity1 Centre in Birmingham, UK. Prehospital physician availability increased from 12 to 24   h after March 2012, and tasking became centralized. Data included demographics, mechanism of injury, crew personnel, and details of @entity2 . The periods before and after the establishment of the regional MTN were compared. RESULTS: There were 5244 @entity0 : 1432 (27.3%) before and 3812 (72.7%) after March 2012. Of the @entity0 , 67.2% were male. The most common injuries were road traffic collisions (45.0%). Physicians were more likely to be present after (2345 missions, 61.5%) than before (529 missions, 36.9%) March 2012 (P<0.01). @entity2 was performed 434 (8.3%) times and was more likely during the latter than the former period [359 (9.4%) vs. 75 (5.2%), P<0.01]. Successful tracheal intubation was achieved in 99% of occasions. CONCLUSION: The establishment of regional MTNs enables centrally tasked, 24-h physician availability for emergency @entity1 @entity0 . There has been an increase in physician presence on emergency missions and increased frequency of @entity2 for at-risk @entity1 @entity0 at times when there may previously have been an unmet requirement.

title: Prehospital rapid sequence induction following XXXX in the era of regional networks for major @entity1 .

answer: @entity1


abstract: OBJECTIVE: @entity2 ( @entity2 ) provides prompt airway control during emergency evacuation of @entity1 @entity0 . Physicians may be tasked to travel with paramedic ambulance crews to the scene of injury when @entity2 is more likely to be required. This study investigates whether there are any differences in the practice of @entity2 during emergency retrievals for @entity1 since the establishment of the regional @entity1 Network (MTN) in March 2012. METHODS: A retrospective observational study examined prehospital records for all @entity1 @entity0 from September 2010 to January 2015 at a @entity1 Centre in Birmingham, UK. Prehospital physician availability increased from 12 to 24   h after March 2012, and tasking became centralized. Data included demographics, mechanism of injury, crew personnel, and details of @entity2 . The periods before and after the establishment of the regional MTN were compared. RESULTS: There were 5244 @entity0 : 1432 (27.3%) before and 3812 (72.7%) after March 2012. Of the @entity0 , 67.2% were male. The most common injuries were road traffic collisions (45.0%). Physicians were more likely to be present after (2345 missions, 61.5%) than before (529 missions, 36.9%) March 2012 (P<0.01). @entity2 was performed 434 (8.3%) times and was more likely during the latter than the former period [359 (9.4%) vs. 75 (5.2%), P<0.01]. Successful tracheal intubation was achieved in 99% of occasions. CONCLUSION: The establishment of regional MTNs enables centrally tasked, 24-h physician availability for emergency @entity1 @entity0 . There has been an increase in physician presence on emergency missions and increased frequency of @entity2 for at-risk @entity1 @entity0 at times when there may previously have been an unmet requirement.

title: Prehospital rapid sequence induction following @entity1 in the era of regional networks for major XXXX .

answer: @entity1


abstract: PURPOSE OF REVIEW: Currently, the only potential curative therapy for @entity4 ( @entity4 ) is allogeneic hematopoietic stem cell transplantation (alloSCT). As alloSCT confers both a short-term mortality risk compared with conservative management, and a longer-term risk of chronic health burden from @entity3 , careful @entity0 selection for alloSCT is essential. Here, we review data on disease burden and transplant outcomes in @entity4 , describing which @entity0 stand to benefit most (and least) from alloSCT. Evidence for timing of alloSCT and modifying @entity1 burden prior to transplant is also discussed. RECENT FINDINGS: Although @entity0 who undergo alloSCT with excess blasts have poorer outcomes than those transplanted with fewer blasts, the effect of clone size in @entity0 with @entity4 without increased blast proportion is poorly studied, and it is also not yet known whether posttransplant outcomes can be modified with pretransplant therapy such as intensive induction chemotherapy or hypomethylating agent treatment. Randomized data are lacking. Most hematopoietic cells in @entity0 with @entity4 are clonal, even in cases without increased marrow blast proportion. Certain high-risk point mutations such as @entity2 seem to be associated with a worse outcome even when subclonal. SUMMARY: @entity0 with more than 10% blasts should be considered for cytoreductive therapy before transplant, especially if reduced intensity conditioning (alloSCT) is planned. @entity0 with less than 10% blasts who are appropriate candidates for transplant can proceed straight to transplant, though it appears better to delay transplant for those with lower-risk disease. Hypomethylating agent therapy may be useful as a bridge to transplant. Randomized data are eagerly awaited.

title: Allogeneic stem cell transplantation in XXXX : does pretransplant clonal burden matter?

answer: @entity4


abstract: UNASSIGNED: @entity4 emergency usually appears in older @entity0 with previous recurrent episodes, and is among the most frequent admissions to emergency departments. A 29-year-old @entity0 was referred to our clinic with the diagnosis of @entity4 emergency. The @entity0 complained of severe @entity1 , @entity10 , palpitation, diaphoresis, and @entity7 due to @entity4 @entity3 . Her blood pressure was 250/150 mmHg on admission. At the referral hospital, the @entity0 had undergone cranial CT because of her confused state and this excluded @entity14 . Also at that hospital, thoracoabdominal CT for differential diagnosis depicted an @entity6 with a @entity2 core. After admission to our clinic, initial control of excessive blood pressure was not achieved despite high dose intravenous @entity13 therapy. Thereafter intravenous @entity16 treatment was initiated simultaneously with oral alpha blocker therapy in order to counterbalance the unopposed alpha adrenergic activity with beta blocker therapy. After 12 hours, sudden onset of @entity5 developed and deepened despite IV saline, inotropic and vasopressor agents such as IV @entity8 , @entity9 and @entity11 . The @entity0 died at the 24th hour due to hemodynamic collapse as a result of hyperadrenergic state due to possible @entity15 . This case is an exceptional example of @entity4 emergency secondary to fulminant @entity15 failing to respond to intensive antihypertensive treatment, and in which @entity0 death was unavoidable due to uncontrolled excessive adrenergic activity which led to profound @entity12 .

title: XXXX emergency due to @entity15 complicated with refractory hemodynamic collapse.

answer: @entity4


abstract: UNASSIGNED: @entity4 emergency usually appears in older @entity0 with previous recurrent episodes, and is among the most frequent admissions to emergency departments. A 29-year-old @entity0 was referred to our clinic with the diagnosis of @entity4 emergency. The @entity0 complained of severe @entity1 , @entity10 , palpitation, diaphoresis, and @entity7 due to @entity4 @entity3 . Her blood pressure was 250/150 mmHg on admission. At the referral hospital, the @entity0 had undergone cranial CT because of her confused state and this excluded @entity14 . Also at that hospital, thoracoabdominal CT for differential diagnosis depicted an @entity6 with a @entity2 core. After admission to our clinic, initial control of excessive blood pressure was not achieved despite high dose intravenous @entity13 therapy. Thereafter intravenous @entity16 treatment was initiated simultaneously with oral alpha blocker therapy in order to counterbalance the unopposed alpha adrenergic activity with beta blocker therapy. After 12 hours, sudden onset of @entity5 developed and deepened despite IV saline, inotropic and vasopressor agents such as IV @entity8 , @entity9 and @entity11 . The @entity0 died at the 24th hour due to hemodynamic collapse as a result of hyperadrenergic state due to possible @entity15 . This case is an exceptional example of @entity4 emergency secondary to fulminant @entity15 failing to respond to intensive antihypertensive treatment, and in which @entity0 death was unavoidable due to uncontrolled excessive adrenergic activity which led to profound @entity12 .

title: @entity4 emergency due to XXXX complicated with refractory hemodynamic collapse.

answer: @entity15


abstract: @entity2 is the commonest @entity1 in @entity0 that causes significant morbidity and mortality worldwide. Screening by digital rectal examination (DRE) and serum @entity5 (PSA) is used despite its limitations. Gray-scale transrectal ultrasound (TRUS), used to guide multiple random prostatic biopsies, misses up to 20% @entity1 and frequently underestimates the grade of @entity1 . Increasing the number of biopsy cores marginally increases the yield. Evolving techniques of real-time ultrasound elastography (RTE) and contrast-enhanced ultrasound (CEUS) are being investigated to better detect and improve the yield by allowing "targeted" biopsies. Last decade has witnessed rapid developments in magnetic resonance imaging (MRI) for improved management of @entity2 . In addition to the anatomical information, it is capable of providing functional information through diffusion-weighted imaging (DWI), magnetic resonance spectroscopy (MRS), and dynamic contrast-enhanced (DCE) MRI. Multi-parametric MRI has the potential to exclude a significant @entity1 in majority of cases. Inclusion of MRI before prostatic biopsy can reduce the invasiveness of the procedure by limiting the number of cores needed to make a diagnosis and support watchful waiting in others. It is made possible by targeted biopsies as opposed to random. With the availability of minimally invasive therapeutic modalities like high-intensity focused ultrasound (HIFU) and interstitial laser therapy, detecting @entity1 is even more relevant today. @entity3 positron emission tomography/computed tomography ((18)FDG PET/CT) has no role in the initial evaluation of @entity2 . Choline PET has been recently found to be more useful. @entity4 -PET has a higher sensitivity and resolution than a conventional radionuclide bone scan in detecting skeletal metastases.

title: Imaging and intervention in XXXX : Current perspectives and future trends.

answer: @entity2


abstract: INTRODUCTION: @entity11 ( @entity11 ) is characterized by rapidly progressive @entity8 , @entity5 symptoms and @entity6 . We describe the clinical spectrum, underlying etiology and long-term follow-up of @entity0 with @entity11 from India. MATERIALS AND METHODS: This prospective study included @entity0 during the period of January 2009 and December 2011 with the clinical features consistent with @entity11 with one or more of the following: (1) Magnetic resonance imaging (MRI) evidence of temporal lobe involvement; (2) cerebrospinal fluid inflammatory abnormalities, or (3) detection of antineuronal antibodies. Patients with @entity1 , infection, metabolic and nutritional deficits, @entity3 , were excluded. RESULTS: There were 16 @entity0 (9 females), mean age of presentation was 36.6 years (range 15-69 years). The mean duration of symptoms before presentation was 11 months (range 5 days-2 years). The most common symptom at presentation was @entity4 in 7 @entity0 followed by @entity6 in 5 and behavioral changes in three. Nine @entity0 had @entity6 , 11 had change in behavior, language involvement in eight, cerebellar features in 3 and @entity9 in two. Four @entity0 had associated @entity2 , 3 of four presented with neurological symptoms and on investigations found to be have @entity2 . Antineuronal antibody testing was done in 6 of 12 non @entity10 and two @entity10 @entity0 , one positive for @entity7 and one for anti-Hu antibody. MRI brain showed typical fluid attenuated inversion recovery or T2 bilateral temporal lobe hyperintensities in 50% of @entity0 . At a mean follow-up of 21 months (3-36 months), 10 @entity0 improved, 4 @entity0 remained same and two @entity0 expired. CONCLUSION: Early recognition of @entity11 is important based upon clinical, MRI data in the absence of antineuronal surface antibody screen in developing nations. Early institution of immunotherapy will help in improvement in outcome of these @entity0 in long-term.

title: XXXX : Clinical spectrum and long-term outcome from a developing country perspective.

answer: @entity11


abstract: AIM: To follow up @entity0 with @entity5 ( @entity5 ) to assess their outcome and identify an optimal surveillance interval. METHODS: Data obtained prospectively were analyzed in a retrospective manner. @entity0 with clinical evidence of chronic @entity4 ( @entity3 in the epigastrium, @entity6 , and @entity2 ), endoscopic ultrasound (EUS) criteria > 4, and EUS-fine needle aspiration (FNA) were included. A @entity7 was defined as a non-neoplastic space-occupying lesion, a cause of chronic @entity4 that may mimic changes typical of @entity4 on CT or endoscopic ultrasound but without histological evidence. A real @entity1 was defined as a neoplastic space-occupying lesion because of @entity4 confirmed by histology. RESULTS: Thirty-five @entity0 with chronic @entity4 were included, 26 (74.2%) of whom were @entity0 . Nine (25.7%) @entity0 were diagnosed with @entity5 and two (2/35; 5.7%) @entity0 with @entity5 were diagnosed with @entity4 on follow-up. The time between the diagnosis of @entity5 and @entity4 was 35 and 30 d in the two @entity0 . Definitive diagnosis of @entity4 was made by surgery. In the remaining six @entity0 with @entity5 , the median of follow-up was 11 mo (range 1-22 mo) and they showed no evidence of @entity1 on surveillance. In the follow-up of @entity0 without @entity5 but with chronic @entity4 , none were diagnosed with @entity4 . According to our data, older @entity0 with chronic @entity4 are at risk of @entity5 . CONCLUSION: According to characteristics of @entity0 , detection of @entity5 should lead a surveillance program for @entity4 with EUS-FNA in < 1 mo or directly to surgical resection.

title: Follow-up of @entity0 with XXXX : outcome and surveillance.

answer: @entity5


abstract: AIM: To follow up @entity0 with @entity5 ( @entity5 ) to assess their outcome and identify an optimal surveillance interval. METHODS: Data obtained prospectively were analyzed in a retrospective manner. @entity0 with clinical evidence of chronic @entity4 ( @entity3 in the epigastrium, @entity6 , and @entity2 ), endoscopic ultrasound (EUS) criteria > 4, and EUS-fine needle aspiration (FNA) were included. A @entity7 was defined as a non-neoplastic space-occupying lesion, a cause of chronic @entity4 that may mimic changes typical of @entity4 on CT or endoscopic ultrasound but without histological evidence. A real @entity1 was defined as a neoplastic space-occupying lesion because of @entity4 confirmed by histology. RESULTS: Thirty-five @entity0 with chronic @entity4 were included, 26 (74.2%) of whom were @entity0 . Nine (25.7%) @entity0 were diagnosed with @entity5 and two (2/35; 5.7%) @entity0 with @entity5 were diagnosed with @entity4 on follow-up. The time between the diagnosis of @entity5 and @entity4 was 35 and 30 d in the two @entity0 . Definitive diagnosis of @entity4 was made by surgery. In the remaining six @entity0 with @entity5 , the median of follow-up was 11 mo (range 1-22 mo) and they showed no evidence of @entity1 on surveillance. In the follow-up of @entity0 without @entity5 but with chronic @entity4 , none were diagnosed with @entity4 . According to our data, older @entity0 with chronic @entity4 are at risk of @entity5 . CONCLUSION: According to characteristics of @entity0 , detection of @entity5 should lead a surveillance program for @entity4 with EUS-FNA in < 1 mo or directly to surgical resection.

title: Follow-up of XXXX with @entity5 : outcome and surveillance.

answer: @entity0


abstract: OBJECTIVES: To determine the proportion of @entity1 caused by extended-spectrum   -lactamase ( @entity6 )-producing @entity4 or @entity2 Gram-negative organisms in the pediatric intensive care unit (PICU), and to identify risk factors for these @entity1 . METHODS: A retrospective, single-center chart review of @entity0 admitted to a PICU in a 5-year period with @entity1 caused by @entity4 species or @entity2 was completed. Data collected include demographics, length of stay, outcome, and relevant risk factors previously defined in the literature. RESULTS: A total of 110 isolates were cultured from 94 @entity0 . A total of 53% of the isolates were @entity2 , and the remainder were @entity4 subspecies. Of the 110 isolates, 13 isolates (11.8%) in 7 @entity0 were @entity6 positive. The @entity6 -producing isolates were equally distributed amongE coli and @entity4 and were primarily cultured from tracheal aspirates. Most of the @entity6 -positive isolates (9 of 13; 69%) were cultured from @entity0 who received @entity5 and/or @entity3 in the preceding 30 days. @entity0 infected with @entity2 had higher PRISM 1 scores and were more likely to have a Foley catheter, whereas @entity4 were more common in mechanically ventilated males. Although not statistically significant, 80% of @entity0 who were infected with non- @entity6 -producing organisms survived to hospital discharge versus 57% of those infected with @entity6 -producing @entity2 and @entity4 . CONCLUSIONS: Almost 12% of @entity2 and @entity4 isolates in this @entity0 population tested positive for @entity6 production. @entity6 production was equally distributed between @entity2 and @entity4 species. These organisms were cultured from 7% of the study @entity0 . As reported in previous studies, @entity0 infected with @entity6 -producing organisms most often had received prior cephalosporins and had a longer length of stay in the PICU.

title: Epidemiology of XXXX due to extended-spectrum Beta-lactamase-producing bacteria in a pediatric intensive care unit.

answer: @entity1


abstract: OBJECTIVE: To confirm the improvement in arterial endothelial function by aerobic exercise training, @entity1 ( @entity1 ) was tested by ultrasonography. METHODS: @entity0 who received percutaneous coronary intervention due to @entity3 were included. The @entity0 who participated in cardiac rehabilitation (CR) program were categorized as the CR group, and others who did not participate as the control. Both groups underwent initial graded exercise test (GXT) and @entity1 testing. Subsequently, the CR group performed aerobic exercise training sessions. @entity0 in control only received advice regarding the exercise methods. After six weeks, both groups received follow-up GXT and @entity1 testing. RESULTS: There were 16 @entity0 in each group. There were no significant differences in the general characteristics between the groups. The VO2peak was 28.6  4.7 mL/kg/min in the CR group and 31.5  7.4 mL/kg/min in the control at first GXT, and was 31.1  5.1 ml/kg/min in the CR group and 31.4  6.0 ml/kg/min in the control at the follow-up GXT in six weeks. There was a statistically significant improvement in VO2peak only for CR group @entity0 . @entity1 value was 7.59%  1.26% in the CR group, 7.36%  1.48% in the control at first and 9.46%  1.82% in the CR group, and 8.31%  2.04% in the control after six weeks. There was a statistically significant improvement in @entity1 value in the CR group. CONCLUSION: According to the results of GXT and @entity1 testing, six-week exercise-based CR program improved VO2peak and endothelial functions significantly. Thus, exercise-based CR program is necessary in @entity0 with @entity2 .

title: Impact of aerobic exercise training on endothelial function in XXXX .

answer: @entity3


abstract: UNASSIGNED: Cellular senescence is a state of irreversible growth arrest that can be triggered by multiple mechanisms, including telomere @entity5 , the epigenetic derepression of the @entity2 locus and DNA damage. Senescence has been considered a @entity1  -suppressing mechanism that permanently arrests cells at risk for malignant transformation. However, accumulating evidence shows that senescent cells have deleterious effects on the tissue microenvironment. Some of these effects could be attributed to the senescence  -associated secretory phenotype that has the ability to promote @entity1 progression. However, secreted proteins from senescent @entity1 cells and their effects on the @entity1 microenvironment due to ionizing radiation (IR) exposure have not yet been fully elucidated. In the present study, we analyzed cytokines secreted from IR  -induced senescent MCF7 cells by using cytokine microarrays and confirmed by western blot analysis that increased secretion of @entity3 ( @entity3 ), @entity7  ( @entity7 ) and @entity4 ( @entity4 ) occurs in these cells. Invasive, migratory and wound  -healing activities were observed in MDA  -MB  -231 and MCF  -10A cells following treatment with recombinant @entity0 @entity3 , @entity7 and @entity4 proteins. Additionally, tube  -formation activity was assessed in @entity3  -, @entity7  - and @entity4  -treated @entity0 umbilical vein endothelial cells (HUVECs). We found that @entity3 , @entity7 and @entity4 affected cell motility and tube  -formation activity. Since @entity3 markedly affected cell motility, we examined the effect of senescent conditioned media containing neutralizing @entity3 antibodies on @entity6 and wound  -healing activity. Our results demonstrated that IR  -induced senescent @entity1 cells influence the @entity1 microenvironment by increasing the production of cytokines, such as @entity3 , @entity7 and @entity4 . Furthermore, these data suggest that @entity3 is likely a promising target capable of reducing the deleterious effects on the @entity1 microenvironment during radiation therapy.

title: Identification of novel therapeutic targets in the secretome of ionizing radiation  -induced senescent XXXX cells.

answer: @entity1


abstract: @entity4 and @entity5 are associated with bevacizumab, an inhibitor of @entity2 that is widely used to treat many types of @entity1 . As specific types of @entity4 and @entity5 , cerebrovascular events such as central nervous system (CNS) @entity4 events and CNS @entity5 are serious adverse events. However, increased cerebrovascular events have not been uniformly reported by previous studies. New randomized controlled trials (RCTs) have been reported in recent years and we therefore conducted an up-to-date meta-analysis of RCTs to fully characterize the risk of cerebrovascular events with bevacizumab. We searched the databases of PubMed, Web of Science, and the American Society of Clinical Oncology conferences to identify relevant clinical trials up to February 2014. Eligible studies included prospective RCTs that directly compared @entity0 with @entity1 treated with and without bevacizumab. A total of 12,917 @entity0 from 17 RCTs were included in our analysis. @entity0 treated with bevacizumab had a significantly increased risk of cerebrovascular events compared with @entity0 treated with control medication, with a relative risk of 3.28 (95% CI, 1.97-5.48). The risks of CNS @entity4 events and CNS @entity5 were increased compared with control, with RRs of 3.22 (95% CI, 1.71-6.07) and 3.09 (95% CI, 1.36-6.99), respectively. Risk varied with the bevacizumab dose, with RRs of 3.97 (95% CI, 2.15-7.36) and 1.96 (95% CI, 0.76-5.06) at 5 and 2.5 mg/kg/week, respectively. Higher risks were observed in @entity0 with metastatic @entity3 (RR, 6.42; 95% CI, 1.76-35.57), and no significant risk was observed in other types of @entity1 . In conclusion, the addition of bevacizumab significantly increased the risk of cerebrovascular events compared with controls, including CNS @entity4 events and CNS @entity5 . The risk may vary with bevacizumab dose and @entity1 type.

title: Increased risk of cerebrovascular events in @entity0 with XXXX treated with bevacizumab: a meta-analysis.

answer: @entity1


abstract: @entity4 and @entity5 are associated with bevacizumab, an inhibitor of @entity2 that is widely used to treat many types of @entity1 . As specific types of @entity4 and @entity5 , cerebrovascular events such as central nervous system (CNS) @entity4 events and CNS @entity5 are serious adverse events. However, increased cerebrovascular events have not been uniformly reported by previous studies. New randomized controlled trials (RCTs) have been reported in recent years and we therefore conducted an up-to-date meta-analysis of RCTs to fully characterize the risk of cerebrovascular events with bevacizumab. We searched the databases of PubMed, Web of Science, and the American Society of Clinical Oncology conferences to identify relevant clinical trials up to February 2014. Eligible studies included prospective RCTs that directly compared @entity0 with @entity1 treated with and without bevacizumab. A total of 12,917 @entity0 from 17 RCTs were included in our analysis. @entity0 treated with bevacizumab had a significantly increased risk of cerebrovascular events compared with @entity0 treated with control medication, with a relative risk of 3.28 (95% CI, 1.97-5.48). The risks of CNS @entity4 events and CNS @entity5 were increased compared with control, with RRs of 3.22 (95% CI, 1.71-6.07) and 3.09 (95% CI, 1.36-6.99), respectively. Risk varied with the bevacizumab dose, with RRs of 3.97 (95% CI, 2.15-7.36) and 1.96 (95% CI, 0.76-5.06) at 5 and 2.5 mg/kg/week, respectively. Higher risks were observed in @entity0 with metastatic @entity3 (RR, 6.42; 95% CI, 1.76-35.57), and no significant risk was observed in other types of @entity1 . In conclusion, the addition of bevacizumab significantly increased the risk of cerebrovascular events compared with controls, including CNS @entity4 events and CNS @entity5 . The risk may vary with bevacizumab dose and @entity1 type.

title: Increased risk of cerebrovascular events in XXXX with @entity1 treated with bevacizumab: a meta-analysis.

answer: @entity0


abstract: BACKGROUND: Ultrasound has been utilized in the evaluation of compressive and traumatic peripheral nerve pathology. OBJECTIVE: To determine whether US can provide comprehensive evaluation of the post-ganglionic brachial plexus in the setting of neonatal @entity3 and whether this information can be used to guide preoperative nerve reconstruction strategies. MATERIALS AND METHODS: In this retrospective cohort study, preoperative brachial plexus ultrasonography was performed in 52 @entity0 with neonatal @entity3 who were being considered for surgery. The 33 @entity0 who had surgery compose the @entity0 cohort. The presence and location of post-ganglionic @entity2 were evaluated by US and compared to the surgical findings. US evaluation of shoulder @entity1 was conducted as an indirect way to assess the integrity of nerves. Finally, we correlated glenohumeral joint laxity to surgical and clinical management. RESULTS: Ultrasound correctly identified 21 of 25 cases of upper trunk and middle trunk @entity2 involvement (84% sensitivity for each). It was 68% sensitive and 40% specific in detection of lower trunk involvement. US identified shoulder @entity1 in 11 of 21 @entity0 evaluated; 8 of these 11 went on to nerve transfer procedures based upon the imaging findings. US identified 3 cases of shoulder joint laxity of the 13 @entity0 evaluated. @entity4 cases were referred for orthopedic evaluation, with 1 @entity0 undergoing shoulder surgery and another requiring casting. CONCLUSION: Ultrasound can provide useful preoperative evaluation of the post-ganglionic brachial plexus in @entity0 with neonatal @entity3 .

title: Utility of ultrasound in noninvasive preoperative workup of neonatal XXXX .

answer: @entity3


abstract: BACKGROUND: @entity0 compliance is critical for successful allergen immunotherapy (AIT). Previous studies suggest that AIT compliance is worse outside of controlled clinical trials, with reported @entity1 ( @entity1 ) and @entity2 ( @entity2 ) noncompliance at 11% to 50% and 3% to 25%, respectively. METHODS: A retrospective review of 384 AIT @entity0 at a single, tertiary care otolaryngic allergy practice evaluated @entity1 and @entity2 compliance, based on treatment stage. @entity1 compliance was defined as the number of 2-week breaks per year or in compliance with their defined schedule: excellent = 2 or fewer; good = 3 to 4; fair = 5 to 6; and poor = 7 or more. Compliance with @entity2 was defined as the number of days vials were refilled within the defined expiration date: excellent = 10 days or fewer; good = 11 to 15 days, fair = 16 to 20 days; and poor = 25 or more days. Fisher exact and chi square tests were used for statistical analysis. RESULTS: Seventy-four @entity1 and 200 @entity2 @entity0 had data appropriate for analysis. Compliance rates were excellent (62%) or good (22%) in 62 @entity1 @entity0 and excellent (31%) or good (35%) in 131 @entity2 @entity0 . Comparing excellent compliance rates, @entity1 @entity0 had a higher rate of excellent compliance at all stages of treatment compared to @entity2 @entity0 (p < 0.05). For @entity1 @entity0 there was no significant difference in excellent compliance rates between escalation, first year of maintenance, and greater than 1 year of maintenance (p > 0.05). CONCLUSION: The results of this study showed higher rates of @entity0 adherence to treatment protocols among @entity1 @entity0 . There was no decrease in @entity1 compliance rates across treatment stages.

title: Immunotherapy compliance: comparison of subcutaneous versus XXXX .

answer: @entity2


abstract: UNASSIGNED: Vascular smooth muscle cell proliferation is a key event in the development of @entity3 , instant @entity4 and other @entity5 . Inhibition of this proliferation could lead to better prevention and treatment of these diseases. This study was designed to investigate the effects and mechanisms of different concentrations of @entity9 (XN) on @entity0 umbilical artery smooth muscle cell (HUASMC) proliferation in vitro. HUASMCs were cultured by the tissue adherent method, passaged three times, and then identified by immunohistochemistry. HUASMCs were then treated with different concentrations of XN (0, 2.76, 27.6 or 276    M), and a @entity7 ( @entity2 ) assay was used to detect the inhibition of HUASMC proliferation. The levels of @entity8 ( @entity8 ) mRNA and protein ( @entity8 -b) were detected on the cell membrane of these treated HUASMCs using RT-PCR and Western blot analysis, respectively. After culturing and passaging three times, 90  % of the cultured cells were identified as HUASMCs by immunohistochemistry. HUASMC proliferation was inhibited by XN in a dose-dependent manner (P  <  0.05). Furthermore, XN dose-dependently decreased the @entity8 mRNA and @entity8 levels on the cell membranes of HUASMCs (P  <  0.05). Thus, the results suggest that XN could become a potent therapeutic agent for regulating VSMC-associated @entity6 such as @entity1 and @entity4 after angioplasty.

title: Inhibited proliferation of @entity0 umbilical artery smooth muscle cells by XXXX .

answer: @entity9


abstract: UNASSIGNED: Vascular smooth muscle cell proliferation is a key event in the development of @entity3 , instant @entity4 and other @entity5 . Inhibition of this proliferation could lead to better prevention and treatment of these diseases. This study was designed to investigate the effects and mechanisms of different concentrations of @entity9 (XN) on @entity0 umbilical artery smooth muscle cell (HUASMC) proliferation in vitro. HUASMCs were cultured by the tissue adherent method, passaged three times, and then identified by immunohistochemistry. HUASMCs were then treated with different concentrations of XN (0, 2.76, 27.6 or 276    M), and a @entity7 ( @entity2 ) assay was used to detect the inhibition of HUASMC proliferation. The levels of @entity8 ( @entity8 ) mRNA and protein ( @entity8 -b) were detected on the cell membrane of these treated HUASMCs using RT-PCR and Western blot analysis, respectively. After culturing and passaging three times, 90  % of the cultured cells were identified as HUASMCs by immunohistochemistry. HUASMC proliferation was inhibited by XN in a dose-dependent manner (P  <  0.05). Furthermore, XN dose-dependently decreased the @entity8 mRNA and @entity8 levels on the cell membranes of HUASMCs (P  <  0.05). Thus, the results suggest that XN could become a potent therapeutic agent for regulating VSMC-associated @entity6 such as @entity1 and @entity4 after angioplasty.

title: Inhibited proliferation of XXXX umbilical artery smooth muscle cells by @entity9 .

answer: @entity0


abstract: BACKGROUND: Heterogeneity of endothelial cells (ECs) is a hallmark of the vascular system which may impact the development and management of @entity3 . Despite the tremendous progress in differentiation of @entity0 embryonic stem cells (hESCs) towards endothelial lineage, differentiation into arterial and venous endothelial phenotypes remains elusive. Additionally, current differentiation strategies are hampered by inefficiency, lack of reproducibility, and use of animal-derived products. METHODS: To direct the differentiation of hESCs to endothelial subtypes, H1- and H9-hESCs were seeded on @entity0 plasma fibronectin and differentiated under chemically defined conditions by sequential modulation of glycogen synthase kinase-3 (GSK-3), @entity4 ( @entity4 ), @entity7 ( @entity7 ) and @entity1 ( @entity1 ) signaling pathways for 5 days. Following the initial differentiation, the endothelial progenitor cells ( @entity6 (+) @entity5 (+) cells) were sorted and terminally differentiated under serum-free conditions to arterial and venous ECs. The transcriptome and secretome profiles of the two distinct populations of hESC-derived arterial and venous ECs were characterized. Furthermore, the safety and functionality of these cells upon in vivo transplantation were characterized. RESULTS: Sequential modulation of hESCs with GSK-3 inhibitor, @entity4 , @entity7 and @entity1 resulted in stages reminiscent of primitive streak, early mesoderm/lateral plate mesoderm, and endothelial progenitors under feeder- and serum-free conditions. Furthermore, these endothelial progenitors demonstrated differentiation potential to almost pure populations of arterial and venous endothelial phenotypes under serum-free conditions. Specifically, the endothelial progenitors differentiated to venous ECs in the absence of @entity1 , and to arterial phenotype under low concentrations of @entity1 . Additionally, these hESC-derived arterial and venous ECs showed distinct molecular and functional profiles in vitro. Furthermore, these hESC-derived arterial and venous ECs were nontumorigenic and were functional in terms of forming perfused microvascular channels upon subcutaneous implantation in the @entity2 . CONCLUSIONS: We report a simple, rapid, and efficient protocol for directed differentiation of hESCs into endothelial progenitor cells capable of differentiation to arterial and venous ECs under feeder-free and serum-free conditions. This could offer a @entity0 platform to study arterial-venous specification for various applications related to drug discovery, disease modeling and regenerative medicine in the future.

title: Efficient differentiation of XXXX embryonic stem cells to arterial and venous endothelial cells under feeder- and serum-free conditions.

answer: @entity0


abstract: UNASSIGNED: There is a paucity of literature reporting the outcome of @entity10 ( @entity10 ) in @entity0 , adolescents, and young adults (CAYA). A multimodal therapeutic approach is commonly used, with no well-established treatment consensus. We conducted a retrospective review of CAYA with @entity10 , treated at our institution, to determine their clinical findings, treatments, and outcomes. Immunohistochemistry ( @entity6 and @entity3 ) and DNA sequencing were performed on 5 @entity1 samples. A literature review of @entity10 was also conducted. We reviewed 13 @entity0 (median age, 7  years) with a primary diagnosis of @entity10 between 1990 and 2015. Diagnoses included unclassified @entity2 (n  =  9), @entity4 (n  =  2), and @entity11 (n  =  2). Five @entity0 underwent upfront gross total resection (GTR) of the @entity1 . The 5-drug regimen ( @entity15 , @entity5 , @entity7 , @entity17 , and @entity13 ) was the most common treatment used. Nine @entity0 died due to progression or recurrence (n  =  8) or secondary malignancy (n  =  1). The median follow-up period of the 4 surviving @entity0 was 1.69  years (range 1.44-5.17  years). The 5-year progression-free survival and overall survival rates were 21 and 44  %, respectively. @entity8 , @entity9 , @entity12 , @entity16 , @entity14 , MET, @entity18 , ATM, and @entity3 mutations were detected in 4 of the 5 tissue samples. All 5 samples were immunopositive for @entity6 , and only 2 were positive for @entity3 . @entity10 remain a therapeutic challenge due to high progression and recurrence rates. Collaborative multi-institutional studies are warranted to delineate a treatment consensus and investigate @entity1 biology to improve the disease outcome.

title: Primary XXXX in @entity0 , adolescents, and young adults: single institution experience and review of the literature.

answer: @entity2


abstract: UNASSIGNED: There is a paucity of literature reporting the outcome of @entity10 ( @entity10 ) in @entity0 , adolescents, and young adults (CAYA). A multimodal therapeutic approach is commonly used, with no well-established treatment consensus. We conducted a retrospective review of CAYA with @entity10 , treated at our institution, to determine their clinical findings, treatments, and outcomes. Immunohistochemistry ( @entity6 and @entity3 ) and DNA sequencing were performed on 5 @entity1 samples. A literature review of @entity10 was also conducted. We reviewed 13 @entity0 (median age, 7  years) with a primary diagnosis of @entity10 between 1990 and 2015. Diagnoses included unclassified @entity2 (n  =  9), @entity4 (n  =  2), and @entity11 (n  =  2). Five @entity0 underwent upfront gross total resection (GTR) of the @entity1 . The 5-drug regimen ( @entity15 , @entity5 , @entity7 , @entity17 , and @entity13 ) was the most common treatment used. Nine @entity0 died due to progression or recurrence (n  =  8) or secondary malignancy (n  =  1). The median follow-up period of the 4 surviving @entity0 was 1.69  years (range 1.44-5.17  years). The 5-year progression-free survival and overall survival rates were 21 and 44  %, respectively. @entity8 , @entity9 , @entity12 , @entity16 , @entity14 , MET, @entity18 , ATM, and @entity3 mutations were detected in 4 of the 5 tissue samples. All 5 samples were immunopositive for @entity6 , and only 2 were positive for @entity3 . @entity10 remain a therapeutic challenge due to high progression and recurrence rates. Collaborative multi-institutional studies are warranted to delineate a treatment consensus and investigate @entity1 biology to improve the disease outcome.

title: Primary @entity2 in XXXX , adolescents, and young adults: single institution experience and review of the literature.

answer: @entity0


abstract: UNASSIGNED: The fact that many chemotherapeutic drugs cause chemoresistance and side effects during the course of @entity2 treatment necessitates development of novel cytotoxic agents aiming to attenuate new molecular targets. Here, we show that Astragalus membranaceus (Fischer) Bge. var. mongolicus (Bge.) Hsiao (AM), a traditional Chinese medicine, can inhibit @entity1 growth in vivo and elucidate the underlying molecular mechanisms. The antitumor effect of AM was assessed on the subcutaneous @entity1 of @entity0 @entity2 cell line HCT116 grafted into @entity3 . The @entity3 were treated with either water or 500 mg/kg AM once per day, before being sacrificed for extraction of @entity1 , which were then subjected to microarray expression profiling. The gene expression of the extraction was then profiled using microarray analysis. The identified genes differentially expressed between treated @entity3 and controls reveal that administration of AM suppresses chromosome organization, histone modification, and regulation of macromolecule metabolic process. A separate analysis focused on differentially expressed microRNAs revealing involvement of macromolecule metabolism, and intracellular transport, as well as several @entity1 signaling pathways. For validation, the input of the identified genes to The Library of Integrated Network-based Cellular Signatures led to many chemopreventive agents of natural origin that produce similar gene expression profiles to that of AM. The demonstrated effectiveness of AM suggests a potential therapeutic drug for @entity2 .

title: An in vivo molecular response analysis of XXXX treated with Astragalus membranaceus extract.

answer: @entity2


abstract: Approximately one-third of @entity7 (LASV)-infected @entity0 develop @entity4 ( @entity4 ) in the late stages of @entity3 or in early convalescence. With 500,000 annual cases of @entity6 ( @entity6 ), LASV is a major cause of @entity2 in regions of West Africa where @entity6 is endemic. To date, no animal models exist that depict the @entity0 pathology of @entity6 with associated @entity2 . Here, we aimed to develop an animal model to study LASV-induced @entity2 using @entity0 isolates from a 2012 Sierra Leone outbreak. We have recently established a @entity1 model for @entity6 that closely mimics many features of @entity0 disease. In this model, LASV isolated from a lethal @entity0 case was highly virulent, while the virus isolated from a nonlethal case elicited mostly mild disease with moderate mortality. More importantly, both viruses were able to induce @entity4 in surviving animals. However, utilization of the nonlethal, @entity0 LASV isolate allowed us to consistently produce large numbers of survivors with @entity2 . Surviving @entity1 developed permanent @entity2 associated with mild damage to the cochlear hair cells and, strikingly, significant degeneration of the @entity5 . Therefore, the pathological changes in the inner ear of the @entity1 with @entity4 supported the phenotypic loss of hearing and provided further insights into the mechanistic cause of @entity6 -associated @entity2 . IMPORTANCE: @entity4 is a major complication for @entity6 survivors. The development of a small-animal model of LASV infection that replicates @entity2 and the clinical and pathological features of @entity6 will significantly increase knowledge of pathogenesis and vaccine studies. In addition, such a model will permit detailed characterization of the @entity2 mechanism and allow for the development of appropriate diagnostic approaches and medical care for @entity6 @entity0 with @entity2 .

title: Animal Model of @entity4 Associated with XXXX Infection.

answer: @entity7


abstract: Approximately one-third of @entity7 (LASV)-infected @entity0 develop @entity4 ( @entity4 ) in the late stages of @entity3 or in early convalescence. With 500,000 annual cases of @entity6 ( @entity6 ), LASV is a major cause of @entity2 in regions of West Africa where @entity6 is endemic. To date, no animal models exist that depict the @entity0 pathology of @entity6 with associated @entity2 . Here, we aimed to develop an animal model to study LASV-induced @entity2 using @entity0 isolates from a 2012 Sierra Leone outbreak. We have recently established a @entity1 model for @entity6 that closely mimics many features of @entity0 disease. In this model, LASV isolated from a lethal @entity0 case was highly virulent, while the virus isolated from a nonlethal case elicited mostly mild disease with moderate mortality. More importantly, both viruses were able to induce @entity4 in surviving animals. However, utilization of the nonlethal, @entity0 LASV isolate allowed us to consistently produce large numbers of survivors with @entity2 . Surviving @entity1 developed permanent @entity2 associated with mild damage to the cochlear hair cells and, strikingly, significant degeneration of the @entity5 . Therefore, the pathological changes in the inner ear of the @entity1 with @entity4 supported the phenotypic loss of hearing and provided further insights into the mechanistic cause of @entity6 -associated @entity2 . IMPORTANCE: @entity4 is a major complication for @entity6 survivors. The development of a small-animal model of LASV infection that replicates @entity2 and the clinical and pathological features of @entity6 will significantly increase knowledge of pathogenesis and vaccine studies. In addition, such a model will permit detailed characterization of the @entity2 mechanism and allow for the development of appropriate diagnostic approaches and medical care for @entity6 @entity0 with @entity2 .

title: Animal Model of XXXX Associated with @entity7 Infection.

answer: @entity4


abstract: BACKGROUND: The immune contexture predicts prognosis in @entity0 @entity2 ( @entity2 ). Whereas @entity1 -infiltrating @entity4 + T cells and myeloid @entity7 + @entity6 ( @entity6 )+ cells are associated with favourable clinical outcome, @entity8 -producing cells have been reported to correlate with severe prognosis. However, their phenotypes and functions continue to be debated. OBJECTIVE: To investigate clinical relevance, phenotypes and functional features of @entity2 , @entity8 -producing cells. METHODS: @entity8 staining was performed by immunohistochemistry on a tissue microarray including 1148 CRCs. Phenotypes of @entity8 -producing cells were evaluated by flow cytometry on cell suspensions obtained by enzymatic digestion of clinical specimens. Functions of @entity2 -isolated, @entity8 -producing cells were assessed by in vitro and in vivo experiments. RESULTS: @entity8 + infiltrates were not themselves predictive of an unfavourable clinical outcome, but correlated with infiltration by @entity4 + T cells and @entity7 + @entity6 + neutrophils. Ex vivo analysis showed that @entity1 -infiltrating @entity8 + cells mostly consist of @entity3 + T helper 17 (Th17) cells with multifaceted properties. Indeed, owing to @entity8 secretion, @entity2 -derived Th17 triggered the release of protumorigenic factors by @entity1 and @entity1 . However, on the other hand, they favoured recruitment of beneficial neutrophils through @entity5 secretion and, most importantly, they drove highly cytotoxic @entity9 + @entity11 + @entity4 + T cells into @entity1 tissue, through @entity10 and @entity12 release. Consistent with these findings, the presence of intraepithelial, but not of stromal Th17 cells, positively correlated with improved survival. CONCLUSIONS: Our study shows the dual role played by @entity1 -infiltrating Th17 in @entity2 , thus advising caution when developing new @entity8 /Th17 targeted treatments.

title: Dual role of @entity1 -infiltrating T helper 17 cells in @entity0 XXXX .

answer: @entity2


abstract: BACKGROUND: The immune contexture predicts prognosis in @entity0 @entity2 ( @entity2 ). Whereas @entity1 -infiltrating @entity4 + T cells and myeloid @entity7 + @entity6 ( @entity6 )+ cells are associated with favourable clinical outcome, @entity8 -producing cells have been reported to correlate with severe prognosis. However, their phenotypes and functions continue to be debated. OBJECTIVE: To investigate clinical relevance, phenotypes and functional features of @entity2 , @entity8 -producing cells. METHODS: @entity8 staining was performed by immunohistochemistry on a tissue microarray including 1148 CRCs. Phenotypes of @entity8 -producing cells were evaluated by flow cytometry on cell suspensions obtained by enzymatic digestion of clinical specimens. Functions of @entity2 -isolated, @entity8 -producing cells were assessed by in vitro and in vivo experiments. RESULTS: @entity8 + infiltrates were not themselves predictive of an unfavourable clinical outcome, but correlated with infiltration by @entity4 + T cells and @entity7 + @entity6 + neutrophils. Ex vivo analysis showed that @entity1 -infiltrating @entity8 + cells mostly consist of @entity3 + T helper 17 (Th17) cells with multifaceted properties. Indeed, owing to @entity8 secretion, @entity2 -derived Th17 triggered the release of protumorigenic factors by @entity1 and @entity1 . However, on the other hand, they favoured recruitment of beneficial neutrophils through @entity5 secretion and, most importantly, they drove highly cytotoxic @entity9 + @entity11 + @entity4 + T cells into @entity1 tissue, through @entity10 and @entity12 release. Consistent with these findings, the presence of intraepithelial, but not of stromal Th17 cells, positively correlated with improved survival. CONCLUSIONS: Our study shows the dual role played by @entity1 -infiltrating Th17 in @entity2 , thus advising caution when developing new @entity8 /Th17 targeted treatments.

title: Dual role of XXXX -infiltrating T helper 17 cells in @entity0 @entity2 .

answer: @entity1


abstract: BACKGROUND: The immune contexture predicts prognosis in @entity0 @entity2 ( @entity2 ). Whereas @entity1 -infiltrating @entity4 + T cells and myeloid @entity7 + @entity6 ( @entity6 )+ cells are associated with favourable clinical outcome, @entity8 -producing cells have been reported to correlate with severe prognosis. However, their phenotypes and functions continue to be debated. OBJECTIVE: To investigate clinical relevance, phenotypes and functional features of @entity2 , @entity8 -producing cells. METHODS: @entity8 staining was performed by immunohistochemistry on a tissue microarray including 1148 CRCs. Phenotypes of @entity8 -producing cells were evaluated by flow cytometry on cell suspensions obtained by enzymatic digestion of clinical specimens. Functions of @entity2 -isolated, @entity8 -producing cells were assessed by in vitro and in vivo experiments. RESULTS: @entity8 + infiltrates were not themselves predictive of an unfavourable clinical outcome, but correlated with infiltration by @entity4 + T cells and @entity7 + @entity6 + neutrophils. Ex vivo analysis showed that @entity1 -infiltrating @entity8 + cells mostly consist of @entity3 + T helper 17 (Th17) cells with multifaceted properties. Indeed, owing to @entity8 secretion, @entity2 -derived Th17 triggered the release of protumorigenic factors by @entity1 and @entity1 . However, on the other hand, they favoured recruitment of beneficial neutrophils through @entity5 secretion and, most importantly, they drove highly cytotoxic @entity9 + @entity11 + @entity4 + T cells into @entity1 tissue, through @entity10 and @entity12 release. Consistent with these findings, the presence of intraepithelial, but not of stromal Th17 cells, positively correlated with improved survival. CONCLUSIONS: Our study shows the dual role played by @entity1 -infiltrating Th17 in @entity2 , thus advising caution when developing new @entity8 /Th17 targeted treatments.

title: Dual role of @entity1 -infiltrating T helper 17 cells in XXXX @entity2 .

answer: @entity0


abstract: OBJECTIVES: Totally epicardial cardiac resynchronization therapy (CRT) is a novel treatment modality for @entity0 with @entity3 ( @entity3 ) and systolic dyssynchrony undergoing coronary artery bypass grafting (CABG). In this study, we have prospectively evaluated the long-term outcomes of totally epicardial CRT. METHODS: Between September 2007 and June 2009, one hundred and seventy-eight @entity0 were randomly assigned to the CABG alone group (n = 87) and CABG with concomitant epicardial CRT implantation (n = 91). The primary end-point of the study was all-cause mortality in the two groups between the day of surgery and 13 August 2013 (common closing date). The secondary outcomes included mode of @entity2 , adverse cardiac events and lead performance. RESULTS: The mean follow-up was 55    10.7 months. According to per-protocol analysis with treatment as a time-dependent variable to account for conversion from CABG to CABG + CRT, there were 24 @entity2 (35.8%) in the CABG group and 17 @entity2 (15.3%) in the CABG + CRT group. When compared with CABG alone, concomitant CRT was associated with reduced risk of both all-cause mortality [hazard ratio (HR) 0.43, 95% confidence interval (CI) 0.23-0.84, P = 0.012] and @entity2 (HR 0.39, 95% CI 0.21-0.72, P = 0.002). Eleven (12.6%) sudden @entity2 were observed in the CABG group in comparison with 4 (4.4%) in the CABG + CRT group (P = 0.048). Hospital readmission was required for 9 (9.9%) @entity0 in CABG + CRT group and for 25 (28.7%) @entity0 in the CABG group (P = 0.001). There were 4 (1.5%) @entity1 . CONCLUSIONS: The results of our study suggest that the procedure of CABG and totally epicardial CRT system implantation is safe and significantly improves the survival of @entity0 with @entity3 and dyssynchrony during long-term follow-up. CLINICAL TRIAL REGISTRATION: NCT 00846001 (http://www.clinicaltrials.gov).

title: Cardiac resynchronization therapy combined with coronary artery bypass grafting in XXXX @entity0 : long-term results of the RESCUE study.

answer: @entity3


abstract: OBJECTIVES: Totally epicardial cardiac resynchronization therapy (CRT) is a novel treatment modality for @entity0 with @entity3 ( @entity3 ) and systolic dyssynchrony undergoing coronary artery bypass grafting (CABG). In this study, we have prospectively evaluated the long-term outcomes of totally epicardial CRT. METHODS: Between September 2007 and June 2009, one hundred and seventy-eight @entity0 were randomly assigned to the CABG alone group (n = 87) and CABG with concomitant epicardial CRT implantation (n = 91). The primary end-point of the study was all-cause mortality in the two groups between the day of surgery and 13 August 2013 (common closing date). The secondary outcomes included mode of @entity2 , adverse cardiac events and lead performance. RESULTS: The mean follow-up was 55    10.7 months. According to per-protocol analysis with treatment as a time-dependent variable to account for conversion from CABG to CABG + CRT, there were 24 @entity2 (35.8%) in the CABG group and 17 @entity2 (15.3%) in the CABG + CRT group. When compared with CABG alone, concomitant CRT was associated with reduced risk of both all-cause mortality [hazard ratio (HR) 0.43, 95% confidence interval (CI) 0.23-0.84, P = 0.012] and @entity2 (HR 0.39, 95% CI 0.21-0.72, P = 0.002). Eleven (12.6%) sudden @entity2 were observed in the CABG group in comparison with 4 (4.4%) in the CABG + CRT group (P = 0.048). Hospital readmission was required for 9 (9.9%) @entity0 in CABG + CRT group and for 25 (28.7%) @entity0 in the CABG group (P = 0.001). There were 4 (1.5%) @entity1 . CONCLUSIONS: The results of our study suggest that the procedure of CABG and totally epicardial CRT system implantation is safe and significantly improves the survival of @entity0 with @entity3 and dyssynchrony during long-term follow-up. CLINICAL TRIAL REGISTRATION: NCT 00846001 (http://www.clinicaltrials.gov).

title: Cardiac resynchronization therapy combined with coronary artery bypass grafting in @entity3 XXXX : long-term results of the RESCUE study.

answer: @entity0


abstract: UNASSIGNED: Currently, few techniques are available for the evaluation of bacterial biofilm adhesion. These detection tools generally require time for culture and/or arduous handling steps.In this work, the Biofilm Ring Test   (BRT), a new technology, was used to estimate the biofilm formation kinetics of twenty-five strains of @entity1 , isolated from sputum of @entity3 ( @entity3 ) @entity0 . The new assay principle is based on the mobility measurement of magnetic microbeads mixed with a bacterial suspension in a @entity2 microplate. If free to move under the magnet action, particles gather to a visible central spot in the well bottom. Therefore, the absence of spot formation in the plate reflects the bead immobilisation by a biofilm in formation.The BRT device allowed us to classify the bacterial strains in three general adhesion profiles. The group 1 consists in bacteria, which are able to form a solid biofilm in less than two hours. The group 2 comprises the strains, which progressively set up a biofilm during 24h. Lastly, the group 3 includes the strains, which stay in a planktonic form. The belonging of our strains to these profiles didn't differ according to culture conditions as the use of different sets of beads or culture media.The BRT is shown to be an informative tool for the characterization of biofilm-forming bacteria. Various application perspectives could be investigated for that device, such as the addition of antibiotics to the bacterial suspension to select the ones, which would have the ability to inhibit the biofilm formation.

title: The Biofilm Ring Test  : a rapid method for the routine analysis of XXXX biofilm formation kinetics.

answer: @entity1


abstract: @entity2 ( @entity2 ) and @entity4 are inversely related phenomena. We tested whether ploidy status influences the clinical impact of @entity2 in @entity3 ( @entity3 ). We analyzed 167 EECs for @entity2 and ploidy. @entity0 were classified in three categories according to @entity2 and ploidy status. Associations with clinicopathological and molecular variables, survival, and treatment response were assessed. All @entity0 (23%) were scored as diploid, and 14% of microsatellite stable (MSS) @entity0 presented @entity4 . @entity0 associated with older age at diagnosis, @entity1 , high histological grade, and advanced surgical stage. @entity0 also associated with higher grade and advanced stage. In multivariate survival analysis @entity2 did not influence disease-free survival (DFS) or @entity0 -specific survival (CSS). However, when just @entity0 were considered for the analysis, @entity2 significantly contributed to worse DFS and CSS, and the same was observed for @entity4 when @entity0 were analyzed alone. In @entity0 , a differential response to postoperative radiotherapy (RT) was observed according to @entity2 , since it predicted poor DFS and CSS in the multivariate analysis. We conclude that ploidy status influences the clinical impact of @entity2 in @entity3 . Among @entity0 those with @entity2 have poor clinical outcome and respond worse to RT.

title: XXXX and ploidy status define three categories with distinctive prognostic impact in @entity3 .

answer: @entity2


abstract: @entity2 ( @entity2 ) and @entity4 are inversely related phenomena. We tested whether ploidy status influences the clinical impact of @entity2 in @entity3 ( @entity3 ). We analyzed 167 EECs for @entity2 and ploidy. @entity0 were classified in three categories according to @entity2 and ploidy status. Associations with clinicopathological and molecular variables, survival, and treatment response were assessed. All @entity0 (23%) were scored as diploid, and 14% of microsatellite stable (MSS) @entity0 presented @entity4 . @entity0 associated with older age at diagnosis, @entity1 , high histological grade, and advanced surgical stage. @entity0 also associated with higher grade and advanced stage. In multivariate survival analysis @entity2 did not influence disease-free survival (DFS) or @entity0 -specific survival (CSS). However, when just @entity0 were considered for the analysis, @entity2 significantly contributed to worse DFS and CSS, and the same was observed for @entity4 when @entity0 were analyzed alone. In @entity0 , a differential response to postoperative radiotherapy (RT) was observed according to @entity2 , since it predicted poor DFS and CSS in the multivariate analysis. We conclude that ploidy status influences the clinical impact of @entity2 in @entity3 . Among @entity0 those with @entity2 have poor clinical outcome and respond worse to RT.

title: @entity2 and ploidy status define three categories with distinctive prognostic impact in XXXX .

answer: @entity3


abstract: CONTEXT: Scilla scilloides Druce (Liliaceae) is a folk medicine to treat dermal @entity0 ; however, the medicinal properties of this plant have not been completely established. OBJECTIVE: The current study investigates the potent anti-inflammatory effects of S. scilloides bulbs for its traditional usage using lipoxygenase and hyaluronidase as the @entity0 model. To gain insight into the active constituents, nine homoisoflavones (1-9) were subsequently tested. MATERIALS AND METHODS: Lipoxygenase and hyaluronidase inhibition of @entity2 extract from the bulbs of this plant within 2000     g/mL or homoisoflavones within 1000     M were determined by colorimetric methods. RAW264.7 cells were incubated with 10 or 50     M @entity3 plus lipopolysaccharide (LPS) for 24   h. The culture media were collected and analyzed for determination of the @entity1 (NO) level by the colorimetric Griess method to measure the extent of @entity0 . RESULTS: The extract exhibited inhibitory effects on lipoxygenase and hyaluronidase activities with IC50 values 31.5 and 169     g/mL, respectively. Among the nine homoisoflavones tested, four (1 and 3-5) resulted in 79.3-97.9% higher lipoxygenase inhibition than 6.7-32.7% of the others at 500     M. Calculated IC50 values indicated 5 as the compound responsible for strong lipoxygenase inhibition with 15.8     M as the IC50 value. In the hyaluronidase assay, all homoisoflavones tested at 1000     M demonstrated 16.2-58.0% inhibition. Incubating the cells in the presence of all nine homoisoflavones tested at 50     M significantly suppressed the NO production, downward to 1.5-66.0%, in the LPS-activated macrophage cells as a model. DISCUSSION AND CONCLUSION: These results may indicate a potential role of S. scilloides for anti-inflammatory purposes.

title: Inhibitory effects of the XXXX extract from bulbs of Scilla scilloides on lipoxygenase and hyaluronidase activities.

answer: @entity2


abstract: AIMS: The extended usage of unreamed tibial nailing resulted in reports of an increased rate of complications, especially for the distal portion of the @entity2 . Unreamed nailing favours biology at the expense of the achievable mechanical stability, it is therefore of interest to define the limits of the clinical indications for this method. Extra-articular @entity1 of the distal tibial metaphysis, meta-diaphyseal junction, and adjacent diaphysis are distinct in their management from impaction derived '' @entity1 and @entity1 . The goals of this work were to gain a thorough understanding of the load-sharing mechanism between unreamed nail and bones in a @entity1 . With this purpose a complete model of the @entity0 leg was realised, simulating a mid-diaphyseal fracture, classified as A2 type 1, according to the AO classification. The analysis of the entire chain allows to have a complete picture of the stress distribution and of the most stressed bones and soft tissues, but, more importantly can overcome problems connected with boundary conditions imposed at single bony components. METHODS: Model consists of six bony structures: pelvis, femur, @entity3 , fibula, @entity2 , and a simplified lump of the feet, configured in a standing up position. Their articular cartilage layers, were simulated by 3D membranes of opportune stiffness connecting the different segments. Moreover an unreamed intra-medullary nail Expert Tibial Nail (DePuy Synthes(  )) stabilized the @entity1 . A load of 700  N has been applied at the top of pelvis and a part the feet, at the tip, was rigidly fixed. Five different contact interfaces have been imposed at the different bony surfaces in contact. RESULTS: Three different conditions were analysed: the initially healthy @entity2 , the A2 type 1 @entity1 with the Expert tibial nail implanted, and the follow up stage after complete healing of @entity2 . Non-linear finite element analysis of the models were performed with Abaqus version 5.4 (Hibbitt, Karlsson and Sorensen, Inc., Pawtucket, RI) using the geometric non linearity and automatic time stepping options. CONCLUSION: The obtained results reveal interesting consequences deriving by taking into account how the stress shielding can influence the integrity and resistance of bones, in order to identify the mechanical reasons for the unfavourable clinical results, and to identify borderline indications due to biomechanical factors. The evolution of treatment options for these @entity1 has been closely linked to developments in implant technology and surgical technique. Further developments in this area, particularly with respect to minimally invasive plating techniques and nail design are ongoing.

title: The healing stages of an intramedullary implanted XXXX : A stress strain comparative analysis of the calcification process.

answer: @entity2


abstract: BACKGROUND: @entity2 is one of the most prevalent @entity3 worldwide and is a public health problem. @entity0 is the most widespread species responsible for @entity2 in the Americas, Middle East and Africa. Adult female worms (mated to males) release eggs in the hepatic portal vasculature and are the principal cause of morbidity. Comparative separate transcriptomes of female and male adult worms were previously assessed with using microarrays and Serial Analysis of Gene Expression (SAGE), thus limiting the possibility of finding novel genes. Moreover, the egg transcriptome was analyzed only once with limited bacterially cloned cDNA libraries. METHODOLOGY/PRINCIPAL FINDINGS: To compare the gene expression of @entity1 eggs, females, and males, we performed RNA-Seq on these three parasite forms using 454/Roche technology and reconstructed the transcriptome using Trinity de novo assembly. The resulting contigs were mapped to the genome and were cross-referenced with predicted Smp genes and H3K4me3 ChIP-Seq public data. For the first time, we obtained separate, unbiased gene expression profiles for @entity1 eggs and female and male adult worms, identifying enriched biological processes and specific enriched functions for each of the three parasite forms. Transcripts with no match to predicted genes were analyzed for their protein-coding potential and the presence of an encoded conserved protein domain. A set of 232 novel protein-coding genes with putative functions related to reproduction, metabolism, and cell biogenesis was detected, which contributes to the understanding of parasite biology. CONCLUSIONS/SIGNIFICANCE: Large-scale RNA-Seq analysis using de novo assembly associated with genome-wide information for histone marks in the vicinity of gene models constitutes a new approach to transcriptome analysis that has not yet been explored in schistosomes. Importantly, all data have been consolidated into a UCSC Genome Browser search- and download-tool (http:// @entity0 .usp.br/). This database provides new ways to explore the schistosome genome and transcriptome and will facilitate molecular research on this important parasite.

title: XXXX Egg, Adult Male and Female Comparative Gene Expression Analysis and Identification of Novel Genes by RNA-Seq.

answer: @entity0


abstract: OBJECTIVE: To determine the impact of processing delay, temperature, and transport tube type on results of quantitative bacterial culture (QBC) of @entity0 urine. DESIGN: Diagnostic test evaluation. SAMPLE: 60 mL of pooled urine from 4 @entity0 , divided into six 10-mL aliquots. PROCEDURES: Urine aliquots were spiked with bacteria from 1 of 6 independent @entity1 cultures to achieve a target bacterial concentration of 10(5) CFUs/mL. One milliliter from each aliquot was transferred into 5 @entity3 -coated clot tubes (SCTs) and 5 urine transport tubes (UTTs). Samples were stored at 4  C (39  F) and 25  C (77  F) for 0, 8, and 24 hours, and then standard QBCs were performed. RESULTS: Median bacterial concentration for urine samples stored in a UTT for 24 hours at 4  C was lower than that for samples stored in an @entity4 under the same conditions. Conversely, a substantial decrease in median bacterial concentration was identified for samples stored for 24 hours in an @entity4 at 25  C, compared with the median concentration for samples stored in a UTT under the same conditions. Median bacterial concentration in samples stored in an @entity4 at 25  C for 24 hours (275 CFUs/mL) was less than the cutoff typically used to define clinically important @entity2 by use of urine samples obtained via cystocentesis (ie, > 1,000 CFUs/mL). CONCLUSIONS AND CLINICAL RELEVANCE: @entity0 urine samples submitted for immediate QBC should be transported in plain sterile tubes such as SCTs. When prolonged (24-hour) storage at room temperature is anticipated, urine samples should be transported in UTTs.

title: Effects of processing delay, temperature, and transport tube type on results of quantitative bacterial culture of XXXX urine.

answer: @entity0


abstract: Importance: Topical @entity3 therapy is used to treat symptomatic @entity1 ( @entity1 ). However, the potential adverse impact of this therapy on the sinus microbiota has not been well quantified. Objective: To determine changes in microbiologic culture results before and after topical @entity3 therapy in @entity0 with @entity1 with medically and surgically refractory disease. Design, Setting, and @entity0 : We performed a retrospective medical chart review for 22 consecutive adults evaluated and treated between January 1, 2012, and January 1, 2014, at an otolaryngology-rhinology clinic at a regional academic medical center. The @entity0 were 14 @entity0 and 8 @entity0 , who had undergone functional endoscopic sinus surgery for @entity1 , and in whom sinus aspirate cultures were performed before and after topical @entity3 therapy for symptomatic disease. Analyses were performed in April 2014. Exposures: @entity0 underwent treatment with saline sinus rinse, with the addition of @entity3 , for at least 1 week. Main Outcomes and Measures: Bacterial isolates from sinus aspirate culture. Results: The @entity0 included 14 @entity0 and 8 @entity0 , 18 to 75 years old, who underwent a mean of 1.9 functional endoscopic sinus surgical procedures. The mean (range) duration of @entity3 therapy was 6 (2-12) weeks. Before @entity3 therapy, cultures from symptomatic @entity0 (14 @entity0 and 8 @entity0 , ages 18-75 years) revealed common bacteria implicated in @entity1 , which are characteristically gram-positive. After @entity3 therapy, cultures from symptomatic @entity0 shifted significantly: 19 were gram-positive vs 3 gram-negative before treatment; 9 were gram-positive vs 13 gram-negative after treatment (P   =   .004), with increased growth of pathogenic gram-negative bacteria and Corynebacterium. Conclusions and Relevance: These data present evidence supporting the distinct abrogation of culturable sinus bacteria after @entity3 rinses, identifying a shift toward increased pathogenic bacteria. Consideration of healthy host microbiome and @entity2 should guide future treatment considerations.

title: Alteration in Bacterial Culture After Treatment With Topical XXXX for Recalcitrant @entity1 .

answer: @entity3


abstract: Importance: Topical @entity3 therapy is used to treat symptomatic @entity1 ( @entity1 ). However, the potential adverse impact of this therapy on the sinus microbiota has not been well quantified. Objective: To determine changes in microbiologic culture results before and after topical @entity3 therapy in @entity0 with @entity1 with medically and surgically refractory disease. Design, Setting, and @entity0 : We performed a retrospective medical chart review for 22 consecutive adults evaluated and treated between January 1, 2012, and January 1, 2014, at an otolaryngology-rhinology clinic at a regional academic medical center. The @entity0 were 14 @entity0 and 8 @entity0 , who had undergone functional endoscopic sinus surgery for @entity1 , and in whom sinus aspirate cultures were performed before and after topical @entity3 therapy for symptomatic disease. Analyses were performed in April 2014. Exposures: @entity0 underwent treatment with saline sinus rinse, with the addition of @entity3 , for at least 1 week. Main Outcomes and Measures: Bacterial isolates from sinus aspirate culture. Results: The @entity0 included 14 @entity0 and 8 @entity0 , 18 to 75 years old, who underwent a mean of 1.9 functional endoscopic sinus surgical procedures. The mean (range) duration of @entity3 therapy was 6 (2-12) weeks. Before @entity3 therapy, cultures from symptomatic @entity0 (14 @entity0 and 8 @entity0 , ages 18-75 years) revealed common bacteria implicated in @entity1 , which are characteristically gram-positive. After @entity3 therapy, cultures from symptomatic @entity0 shifted significantly: 19 were gram-positive vs 3 gram-negative before treatment; 9 were gram-positive vs 13 gram-negative after treatment (P   =   .004), with increased growth of pathogenic gram-negative bacteria and Corynebacterium. Conclusions and Relevance: These data present evidence supporting the distinct abrogation of culturable sinus bacteria after @entity3 rinses, identifying a shift toward increased pathogenic bacteria. Consideration of healthy host microbiome and @entity2 should guide future treatment considerations.

title: Alteration in Bacterial Culture After Treatment With Topical @entity3 for Recalcitrant XXXX .

answer: @entity1