<Ul> <Li> 1 % sulfuric acid alcohol for actinomycetes , nocardia . </Li> <Li> 0.5 -- 1 % sulfuric acid alcohol for oocysts of isospora , cyclospora . </Li> <Li> 0.25 -- 0.5 % sulfuric acid alcohol for bacterial endospores . </Li> <Li> Brucella differential stain -- glacial acetic acid used , no heat applied , secondary stain is loeffler 's methylene blue . </Li> <Li> Kinyoun modification ( or cold Ziehl -- Neelsen technique ) is also available . </Li> <Li> A protocol in which a detergent is substituted for the highly toxic phenol in the fuchsin staining solution . </Li> </Ul>
<Ul> <Li> 1 % sulfuric acid alcohol for actinomycetes , nocardia . </Li> <Li> 0.5 -- 1 % sulfuric acid alcohol for oocysts of isospora , cyclospora . </Li> <Li> 0.25 -- 0.5 % sulfuric acid alcohol for bacterial endospores . </Li> <Li> Brucella differential stain -- glacial acetic acid used , no heat applied , secondary stain is loeffler 's methylene blue . </Li> <Li> Kinyoun modification ( or cold Ziehl -- Neelsen technique ) is also available . </Li> <Li> A protocol in which a detergent is substituted for the highly toxic phenol in the fuchsin staining solution . </Li> </Ul>
what is the secondary stain in the acid fast stain
what is the secondary stain in the acid fast stain
loeffler 's methylene blue
loeffler 's methylene blue