<P> High - performance liquid chromatography ( HPLC ; formerly referred to as high - pressure liquid chromatography ) , is a technique in analytical chemistry used to separate , identify , and quantify each component in a mixture . It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material . Each component in the sample interacts slightly differently with the adsorbent material , causing different flow rates for the different components and leading to the separation of the components as they flow out the column . </P>
<P> High - performance liquid chromatography ( HPLC ; formerly referred to as high - pressure liquid chromatography ) , is a technique in analytical chemistry used to separate , identify , and quantify each component in a mixture . It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material . Each component in the sample interacts slightly differently with the adsorbent material , causing different flow rates for the different components and leading to the separation of the components as they flow out the column . </P>
how hplc is used as a separation technique
how hplc is used as a separation technique
It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material . Each component in the sample interacts slightly differently with the adsorbent material , causing different flow rates for the different components and leading to the separation of the components as they flow out the column .
It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material . Each component in the sample interacts slightly differently with the adsorbent material , causing different flow rates for the different components and leading to the separation of the components as they flow out the column .