[PAR] [TLE] Strep-tag [SEP] The Strep-tag® system is a method which allows the purification and detection of proteins by affinity chromatography.  The Strep-tag II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys).  This peptide sequence exhibits intrinsic affinity towards Strep-Tactin®, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins.  By exploiting the highly specific interaction, "Strep"-tagged proteins can be isolated in one step from crude cell lysates.  Because the "Strep"-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. [PAR] [TLE] Histidine [SEP] Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins.  It contains an α-amino group (which is in the protonated –NH form under biological conditions), a carboxylic acid group (which is in the deprotonated –COO form under biological conditions), and an imidazole side chain (which is partially protonated), classifying it as a positively charged amino acid at physiological pH. Initially thought essential only for infants, longer-term studies have shown it is essential for adults also.
[PAR] [TLE] Strep - tag [ SEP ] The Strep - tag ® system is a method which allows the purification and detection of proteins by affinity chromatography . The Strep - tag II is a synthetic peptide consisting of eight amino acids ( Trp - Ser - His - Pro - Gln - Phe - Glu - Lys ) . This peptide sequence exhibits intrinsic affinity towards Strep - Tactin ® , a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins . By exploiting the highly specific interaction , " Strep"-tagged proteins can be isolated in one step from crude cell lysates . Because the " Strep"-tag elutes under gentle , physiological conditions it is especially suited for generation of functional proteins . [PAR] [TLE] Histidine [ SEP ] Histidine ( abbreviated as His or H ; encoded by the codons CAU and CAC ) is an α - amino acid that is used in the biosynthesis of proteins . It contains an α - amino group ( which is in the protonated –NH form under biological conditions ) , a carboxylic acid group ( which is in the deprotonated –COO form under biological conditions ) , and an imidazole side chain ( which is partially protonated ) , classifying it as a positively charged amino acid at physiological pH. Initially thought essential only for infants , longer - term studies have shown it is essential for adults also .
What is one of the amino acids used in the biosynthesis of proteins, that is in a synthetic peptide consisting of eight amino acids?
What is one of the amino acids used in the biosynthesis of proteins , that is in a synthetic peptide consisting of eight amino acids ?
Histidine
Histidine