<P> In organic chemistry , reactions are qualitatively monitored with TLC . Spots sampled with a capillary tube are placed on the plate : a spot of starting material , a spot from the reaction mixture , and a cross-spot with both . A small ( 3 by 7 cm ) TLC plate takes a couple of minutes to run . The analysis is qualitative , and it will show if the starting material has disappeared , i.e. the reaction is complete , if any product has appeared , and how many products are generated ( although this might be underestimated due to co-elution ) . Unfortunately , TLCs from low - temperature reactions may give misleading results , because the sample is warmed to room temperature in the capillary , which can alter the reaction -- the warmed sample analyzed by TLC is not the same as what is in the low - temperature flask . One such reaction is the DIBALH reduction of ester to aldehyde . </P>
<P> In organic chemistry , reactions are qualitatively monitored with TLC . Spots sampled with a capillary tube are placed on the plate : a spot of starting material , a spot from the reaction mixture , and a cross-spot with both . A small ( 3 by 7 cm ) TLC plate takes a couple of minutes to run . The analysis is qualitative , and it will show if the starting material has disappeared , i.e. the reaction is complete , if any product has appeared , and how many products are generated ( although this might be underestimated due to co-elution ) . Unfortunately , TLCs from low - temperature reactions may give misleading results , because the sample is warmed to room temperature in the capillary , which can alter the reaction -- the warmed sample analyzed by TLC is not the same as what is in the low - temperature flask . One such reaction is the DIBALH reduction of ester to aldehyde . </P>
where is thin layer chromatography used in industry
where is thin layer chromatography used in industry
organic chemistry
organic chemistry