<P> In the early 1980s , Kary Mullis was working at Cetus Corporation on the application of synthetic DNAs to biotechnology . He was familiar with the use of DNA oligonucleotides as probes for binding to target DNA strands , as well as their use as primers for DNA sequencing and cDNA synthesis . In 1983 , he began using two primers , one to hybridize to each strand of a target DNA , and adding DNA polymerase to the reaction . This led to exponential DNA replication , greatly amplifying the amounts of DNA between the primers . </P>
<P> In the early 1980s , Kary Mullis was working at Cetus Corporation on the application of synthetic DNAs to biotechnology . He was familiar with the use of DNA oligonucleotides as probes for binding to target DNA strands , as well as their use as primers for DNA sequencing and cDNA synthesis . In 1983 , he began using two primers , one to hybridize to each strand of a target DNA , and adding DNA polymerase to the reaction . This led to exponential DNA replication , greatly amplifying the amounts of DNA between the primers . </P>
what is the role of dna polymerase in pcr
what is the role of dna polymerase in pcr
DNA replication
DNA replication