<P> Reversed - phase chromatography is a technique using alkyl chains covalently bonded to the stationary phase particles in order to create a hydrophobic stationary phase , which has a stronger affinity for hydrophobic or less polar compounds . The use of a hydrophobic stationary phase is essentially the reverse of normal phase chromatography , since the polarity of the mobile and stationary phases have been inverted -- hence the term reversed - phase chromatography . Reversed - phase chromatography employs a polar ( aqueous ) mobile phase . As a result , hydrophobic molecules in the polar mobile phase tend to adsorb to the hydrophobic stationary phase , and hydrophilic molecules in the mobile phase will pass through the column and are eluted first . Hydrophobic molecules can be eluted from the column by decreasing the polarity of the mobile phase using an organic ( non-polar ) solvent , which reduces hydrophobic interactions . The more hydrophobic the molecule , the more strongly it will bind to the stationary phase , and the higher the concentration of organic solvent that will be required to elute the molecule . </P>
<P> Reversed - phase chromatography is a technique using alkyl chains covalently bonded to the stationary phase particles in order to create a hydrophobic stationary phase , which has a stronger affinity for hydrophobic or less polar compounds . The use of a hydrophobic stationary phase is essentially the reverse of normal phase chromatography , since the polarity of the mobile and stationary phases have been inverted -- hence the term reversed - phase chromatography . Reversed - phase chromatography employs a polar ( aqueous ) mobile phase . As a result , hydrophobic molecules in the polar mobile phase tend to adsorb to the hydrophobic stationary phase , and hydrophilic molecules in the mobile phase will pass through the column and are eluted first . Hydrophobic molecules can be eluted from the column by decreasing the polarity of the mobile phase using an organic ( non-polar ) solvent , which reduces hydrophobic interactions . The more hydrophobic the molecule , the more strongly it will bind to the stationary phase , and the higher the concentration of organic solvent that will be required to elute the molecule . </P>
in reverse phase column chromatography which phase is more polar
in reverse phase column chromatography which phase is more polar
mobile
mobile