<P> In the BCA test , Cu forms a deep purple complex with bicinchoninic acid ( BCA ) , which absorbs around 562 nm , producing the signature violet color . The water - soluble BCA / copper complex absorbs much more strongly than the peptide / copper complex , increasing the sensitivity of the biuret test by a factor of around 100 : the BCA assay allows to detect proteins in the range of 0.0005 to 2 mg / mL ) . Additionally , the BCA protein assay gives the important benefit of compatibility with substances such as up to 5 % surfactants in protein samples . </P>
<P> In the BCA test , Cu forms a deep purple complex with bicinchoninic acid ( BCA ) , which absorbs around 562 nm , producing the signature violet color . The water - soluble BCA / copper complex absorbs much more strongly than the peptide / copper complex , increasing the sensitivity of the biuret test by a factor of around 100 : the BCA assay allows to detect proteins in the range of 0.0005 to 2 mg / mL ) . Additionally , the BCA protein assay gives the important benefit of compatibility with substances such as up to 5 % surfactants in protein samples . </P>
how can you tell by using this test that a substance contains protein
how can you tell by using this test that a substance contains protein
biuret test
biuret test