<P> If you know of a restriction site placed towards one end of the insert you can determine the orientation by observing the size of the fragments in the gel . Often the orientation of inserts is important and this technique is used to screen for the correct orientation . </P>
<P> If you know of a restriction site placed towards one end of the insert you can determine the orientation by observing the size of the fragments in the gel . Often the orientation of inserts is important and this technique is used to screen for the correct orientation . </P>
when constructing an dna molecule what is important when determining the orientation of each strand
when constructing an dna molecule what is important when determining the orientation of each strand
a restriction site placed towards one end of the insert
a restriction site placed towards one end of the insert