<P> Centrifugation is a technique which involves the application of centrifugal force to separate particles from a solution according to their size , shape , density , viscosity of the medium and rotor speed . This process is used to separate two miscible substances , but also to analyze the hydrodynamic properties of macromolecules . More - dense components of the mixture migrate away from the axis of the centrifuge , while less - dense components of the mixture migrate towards the axis . Chemists and biologists may increase the effective gravitational force on a test tube so as to more rapidly and completely cause the precipitate ( pellet ) to gather on the bottom of the tube . The remaining solution ( supernatant ) may be discarded with a pipette. . Centrifugation of protein solution , for example , allows elimination of impurities into the supernatant . </P>
<P> Centrifugation is a technique which involves the application of centrifugal force to separate particles from a solution according to their size , shape , density , viscosity of the medium and rotor speed . This process is used to separate two miscible substances , but also to analyze the hydrodynamic properties of macromolecules . More - dense components of the mixture migrate away from the axis of the centrifuge , while less - dense components of the mixture migrate towards the axis . Chemists and biologists may increase the effective gravitational force on a test tube so as to more rapidly and completely cause the precipitate ( pellet ) to gather on the bottom of the tube . The remaining solution ( supernatant ) may be discarded with a pipette. . Centrifugation of protein solution , for example , allows elimination of impurities into the supernatant . </P>
where does the pellet form when you are centrifuging
where does the pellet form when you are centrifuging
on the bottom of the tube
on the bottom of the tube