<P> Secondary antibody is especially efficient in immunolabeling . Secondary antibodies bind to primary antibodies , which are directly bound to the target antigen ( s ) . In immunolabeling , the primary antibody 's Fab domain binds to an antigen and exposes its Fc domain to secondary antibody . Then , the secondary antibody 's Fab domain binds to the primary antibody 's Fc domain . Since the Fc domain is constant within the same animal class , only one type of secondary antibody is required to bind to many types of primary antibodies . This reduces the cost by labeling only one type of secondary antibody , rather than labeling various types of primary antibodies . Secondary antibodies help increase sensitivity and signal amplification due to multiple secondary antibodies binding to a primary antibody . </P>
<P> Secondary antibody is especially efficient in immunolabeling . Secondary antibodies bind to primary antibodies , which are directly bound to the target antigen ( s ) . In immunolabeling , the primary antibody 's Fab domain binds to an antigen and exposes its Fc domain to secondary antibody . Then , the secondary antibody 's Fab domain binds to the primary antibody 's Fc domain . Since the Fc domain is constant within the same animal class , only one type of secondary antibody is required to bind to many types of primary antibodies . This reduces the cost by labeling only one type of secondary antibody , rather than labeling various types of primary antibodies . Secondary antibodies help increase sensitivity and signal amplification due to multiple secondary antibodies binding to a primary antibody . </P>
where does secondary antibody bind to primary antibody
where does secondary antibody bind to primary antibody
to the primary antibody 's Fc domain
to the primary antibody 's Fc domain