<P> They succeeded in detecting the enzyme activity from the microsomal fraction . This was the crucial step in the serendipitous discovery of lysosomes . To estimate this enzyme activity , they used that of standardised enzyme acid phosphatase , and found that the activity was only 10 % of the expected value . One day , the enzyme activity of purified cell fractions which had been refrigerated for five days was measured . Surprisingly , the enzyme activity was increased to normal of that of the fresh sample . The result was the same no matter how many times they repeated the estimation , and led to the conclusion that a membrane - like barrier limited the accessibility of the enzyme to its substrate , and that the enzymes were able to diffuse after a few days ( and react with their substrate ) . They described this membrane - like barrier as a `` saclike structure surrounded by a membrane and containing acid phosphatase . '' </P>
<P> They succeeded in detecting the enzyme activity from the microsomal fraction . This was the crucial step in the serendipitous discovery of lysosomes . To estimate this enzyme activity , they used that of standardised enzyme acid phosphatase , and found that the activity was only 10 % of the expected value . One day , the enzyme activity of purified cell fractions which had been refrigerated for five days was measured . Surprisingly , the enzyme activity was increased to normal of that of the fresh sample . The result was the same no matter how many times they repeated the estimation , and led to the conclusion that a membrane - like barrier limited the accessibility of the enzyme to its substrate , and that the enzymes were able to diffuse after a few days ( and react with their substrate ) . They described this membrane - like barrier as a `` saclike structure surrounded by a membrane and containing acid phosphatase . '' </P>
what does a lysosome look like in an animal cell
what does a lysosome look like in an animal cell
a `` saclike structure surrounded by a membrane and containing acid phosphatase . ''
a `` saclike structure surrounded by a membrane and containing acid phosphatase . ''