PMC10212754_01
The electropherogram traces of exon 20 S768I positive mutation by pyrosequencing (A) and confirmed negative by Sanger sequencing (reverse) (B)
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Pyrosequencing, using the CE-IVDmarked therascreen EGFR Pyro Kit, on the Qiagen PyroMark Q24 device. Pyrosequencing revealed S768I positive sensitizing EGFR mutation and suggests that a few tumor cells carry the mutation (Fig. 1).