PMC2734935_01
The neonate's neutrophil count pattern and hr-G-CSF therapy course.
CRM2009-717545.001.jpg
PMC2734935_01_CRM2009-717545.001.jpg
fig1
Upon making the diagnosis of neonatal isoimmune neutropenia due to anti-Fc gamma RIIIIb (CD16) pan-reactive antibodies, therapy with rh-GCSF from day 19 to day 23 (Neupogen in a dose of 5 mug/kg/d) was introduced (Figure 1). Neutropenia persisted for 2 months (Figure 1).
HNA-1 genotyping by PCR-SSP method, Electrophoresis of HNA-1 allele PCR products on 1.5% agarose gel for three subjects and three HNA alleles 1a, 1b, and 1c. Mother: lanes 1, 2, and 3, genotype HNA-1a negative, 1b negative, 1c negative; lane 4 molecular marker 100 bp; Father: lanes 5, 6, and 7, genotype is HNA-1a negative, 1b positive, 1c negative; Newborn: lanes 8, 9, and 10, genotype HNA-1a negative, 1b positive, 1c negative; human growth hormone (HGH) gene fragment of 439 bp used as internal positive control; the amplified HNA-1b allele positive product 155 bp.
CRM2009-717545.002.jpg
PMC2734935_01_CRM2009-717545.002.jpg
fig2
Results are shown on Figure 2.