PMC3900044_01
LC-MS/MS digitoxin chromatographic assay.. At 3.77 min, from bottom to top, it is possible to observe chromatographic peaks corresponding respectively to blank, digitoxin standard sample at 20 ng/mL, female sample (50 ng/mL), digitoxin standard sample at 100 ng/mL and male sample (100 ng/mL).
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PMC3900044_01_biochem-med-22-3-380-13f1.jpg
f1-biochem-med-22-3-380-13
Digitoxin was identified in both patients (woman: 50 ng/mL, man: 100 ng/mL) at toxic plasma levels compatible with the severity of the clinical picture (digitoxin therapeutic range: 10-30 ng/mL) (Figure 1).