PMC5303562_01
Temporal lobe staining from AD brain. A) Bielschowsky silver stain demonstrating neuritic plaques and B) PHF-1 stain demonstrating neuritic plaques and neurofibrillary tangles.
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F1
In addition, there were numerous neurofibrillary tangles, particularly in the pyramidal neurons of layers 3 and 5, but no cortical Lewy bodies were present measured by alpha-synuclein staining (Figure 1).
PMC5303562_02
Staining from CTE brain. A) Entorhinal Cortex PHF-1 staining demonstrating neurofibrillary tau tangles. B) Subiculum/hippocampus PHF-1 staining (low power 20x) demonstrating neurofibrillary tau tangles and neuritic neuropil changes. C) Subiculum/hippocampus PHF-1 staining (high power 63x) demonstrating tau neurofibrillary tau tangles. D) Pontine Tegmentum-Locus Ceruleus PHF-1 staining demonstrating neurofibrillary tau tangles.
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F2
The raphe and the surrounding pontine tegmentum contained a few scattered phosphorylated tau positive cells (Figure 2).
PMC5303562_03
Staining from CTE-AD brain. A) Hippocampus Bielschowsky Silver staining demonstrating neurofibrillary tau tangles. B) Temporal Cortex Bielschowsky Silver staining demonstrating amyloid plaques and neurofibrillary tau tangles.
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F3
The H&E and Bielschowsky silver stains revealed numerous amyloid plaques of both the diffuse as well as neuritic type in all neocortical areas (Figure 3).
Staining from CTE-AD brain. A) Temporal cortex PHF-1 staining demonstrating tau neuritic plaques (arrows) and neuritic threads. B) Temporal cortex PHF-1 staining (low power 20x) demonstrating perivascular tauopathy. C) Temporal cortex PHF-1 staining (high power 63x) demonstrating perivascular tauopathy.
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F4
Additionally, characteristic of CTE, perivascular PHF was seen at the depths of the sulci in cortical white matter and the indusium griseum (Figures 4 and 5).
Staining from CTE-AD brain. A) Substantia Nigra PHF-1 staining demonstrating neurofibrillary tau tangles and neuritic neuropil changes. B) Pontine tegmentum PHF-1 staining demonstrating neurofibrillary tau tangles and neuritic neuropil changes. C) Thalamus (Pulvinar) PHF-1 staining demonstrating tau neurofibrillary tangles (arrows) and neuritic neuropil changes.
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F5
Additionally, characteristic of CTE, perivascular PHF was seen at the depths of the sulci in cortical white matter and the indusium griseum (Figures 4 and 5).