PMC9716970_01
A) Bact/alert  fan  aerobic plus; B) bd Bactec  myco/F lytic culture vial
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F1
No bacteria or yeasts were seen on gram stain; however, on subculture of the peritoneal fluid, fungal-like elements were macroscopically visible in the culture bottle (Figure 1A, Figure 1B); and on microscopy hyphal elements suggestive of fungi were seen on 10% potassium hydroxide wet preparation and subsequently Aspergillus fumigatus was isolated (Figure 2, Figure 3, Figure 4, Figure 5).
saboraud's dextrose agar following overnight incubation at 35 C carbon dioxide-enriched incubation
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F2
No bacteria or yeasts were seen on gram stain; however, on subculture of the peritoneal fluid, fungal-like elements were macroscopically visible in the culture bottle (Figure 1A, Figure 1B); and on microscopy hyphal elements suggestive of fungi were seen on 10% potassium hydroxide wet preparation and subsequently Aspergillus fumigatus was isolated (Figure 2, Figure 3, Figure 4, Figure 5).
saboraud's dextrose agar following overnight incubation at 37 C
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PMC9716970_01_PAMJ-43-35-g003.jpg
F3
No bacteria or yeasts were seen on gram stain; however, on subculture of the peritoneal fluid, fungal-like elements were macroscopically visible in the culture bottle (Figure 1A, Figure 1B); and on microscopy hyphal elements suggestive of fungi were seen on 10% potassium hydroxide wet preparation and subsequently Aspergillus fumigatus was isolated (Figure 2, Figure 3, Figure 4, Figure 5).
two percent (2%) tryptose blood agar following overnight incubation at 37 C
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F4
No bacteria or yeasts were seen on gram stain; however, on subculture of the peritoneal fluid, fungal-like elements were macroscopically visible in the culture bottle (Figure 1A, Figure 1B); and on microscopy hyphal elements suggestive of fungi were seen on 10% potassium hydroxide wet preparation and subsequently Aspergillus fumigatus was isolated (Figure 2, Figure 3, Figure 4, Figure 5).
lactophenol cotton-blue stain at 400 times magnification
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PMC9716970_01_PAMJ-43-35-g005.jpg
F5
No bacteria or yeasts were seen on gram stain; however, on subculture of the peritoneal fluid, fungal-like elements were macroscopically visible in the culture bottle (Figure 1A, Figure 1B); and on microscopy hyphal elements suggestive of fungi were seen on 10% potassium hydroxide wet preparation and subsequently Aspergillus fumigatus was isolated (Figure 2, Figure 3, Figure 4, Figure 5).