PMC5592907_01
The results of swollen lymph node biopsy section. (A) Characteristic HRS cells exist in a mixed background of small lymphocytes, the arrow shows HRS cell (x1,000). (B) CD30 positive for lymphoma cells (x200). (C) MUM-1 positive for lymphoma cells (x200). (D) EBER was positive for lymphoma cells and was confirmed by in situ hybridization (x200).. Abbreviations: EBER, Epstein-Barr virus-encoded RNA; HRS cell, Hodgkin and Reed-Sternberg cell.
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Hodgkin and Reed-Sternberg cells were seen in this swollen lymph node biopsy section, and immunohistochemical staining showed that lymphoma cells were positive for CD30, CD15, EMA, and MUM-1 and negative for LCA, MPO, CD117, CD68, CD20, CD3, and TdT; Epstein-Barr virus-encoded RNA was positive for lymphoma cells and was confirmed by in situ hybridization (Figure 1).
The result of BCL/ABL1 fusion gene in swollen lymph node biopsy by FISH (arrow displays that lymphoma cells without BCR/ABL1 fusion genes).. Abbreviation: FISH, fluorescence in situ hybridization.
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BCR and ABL cosmid probes showed that there were no BCR/ABL1 fusion genes in lymph node biopsy section (Figure 2). Based on the abovementioned results, the patient was diagnosed as having classic HL.