PMC5896035_01
PMC5896035_02
Reference mass spectrum from Fermentimonas caenicola SIT8. This reference spectrum was generated by comparison of 12 individual colonies
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Fig1
Using our systematic matrix-assisted laser desorption-ionization time-of-flight screening on a MicroFlex spectrometer (Bruker Daltonics, Bremen, Germany), strain SIT8 exhibited no significant score, suggesting that it was not a member of any known species (Fig. 1). SIT8 to our database (Fig. 1). Strain SIT8 exhibited a 100% 16S rRNA sequence identity with Fermentimonas caenicola strain ING2-E5BT (GenBank accession KP233810), the phylogenetically closest species with a validly published name in nomenclature (Fig. 2).
Phylogenetic tree showing the position of Fermentimonas caenicola strains SIT8 and ING2-E5BT (red) relative to other phylogenetically close members of the family Porphyromonadaceae. GenBank Accession numbers are indicated in parentheses. Sequences were aligned using MUSCLE, and phylogenetic inferences were obtained using the maximum-likelihood method within the MEGA software. Numbers at the nodes are percentages of bootstrap values obtained by repeating the analysis 1000 times to generate a majority consensus tree. Only values >= 70% were displayed. The scale bar indicates a 2% nucleotide sequence divergence
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Fig2
SIT8 to our database (Fig. 1). Strain SIT8 exhibited a 100% 16S rRNA sequence identity with Fermentimonas caenicola strain ING2-E5BT (GenBank accession KP233810), the phylogenetically closest species with a validly published name in nomenclature (Fig. 2).
Gram staining of Fermentimonas caenicola strain SIT8
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Fig3
Cells were Gram-negative, rod-shaped, polymorphic (Fig. 3), unable to form spores and exhibited a mean diameter of 0.35 mum (range 0.3-0.4 mum) and a mean length of 3.8 mum (range 1-8.8 mum) (Fig. 4).
Transmission electron microscopy of Fermentimonas caenicola strain SIT8 using a Morgani 268D microscope (Philips, Amsterdam, The Netherlands) at an operating voltage of 60 kV. Scale bar = 500 nm
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Fig4
Cells were Gram-negative, rod-shaped, polymorphic (Fig. 3), unable to form spores and exhibited a mean diameter of 0.35 mum (range 0.3-0.4 mum) and a mean length of 3.8 mum (range 1-8.8 mum) (Fig. 4).
Graphical circular map of the chromosome of Fermentimonas caenicola strain SIT8. From the outside in, the outer two circles show open reading frames oriented in the forward (coloured by COG categories) and reverse (coloured by COG categories) directions, respectively. The third circle marks the rRNA gene operon (red) and tRNA genes (green). The fourth circle shows the GC % content plot. The inner-most circle shows GC skew, purple indicating negative values and olive for positive values
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Fig5
The genome of strain SIT8 is 2,824,451-bp long with a 37% G + C content (Table 3; Fig. 5).