PMC8577472_01
PMC8577472_02
Flow cytometry plots from the dihydrorhodamine assay. Unstimulated (A) and PMA stimulated (B) neutrophils from a normal control. Patient's neutrophils exhibited the typical CGD XL- carrier bimodal distribution upon PMA stimulation; in 2005, 34% of neutrophils generated normal oxidative activity (C), in 2018, 6% of neutrophils had normal oxidative activity (D).
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PMC8577472_02_gr1.jpg
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The flow cytometric dihydrorhodamine (DHR) assay revealed that only 6% of her neutrophils were able to mount normal oxidative burst, while at the age of 21 years, when her son was diagnosed with CGD, the DHR assay had revealed normal oxidative activity in 34% of her neutrophils (Fig. 1).