PMC4860919_01
Lobular structure of the tumor cells. (H&E stain, x40)
JOMFP-20-151-g001.jpg
PMC4860919_01_JOMFP-20-151-g001.jpg
F1
Under scanner view, the tumor showed multiple lobular patterns separated by dense fibrocellular septae [Figure 1].
Myxoid areas and tumor cells resembling chondroblasts (H&E stain, x100)
JOMFP-20-151-g002.jpg
PMC4860919_01_JOMFP-20-151-g002.jpg
F2
On higher magnification, the tumor cells were seen arranged at the periphery of the lobules assuming a radial pattern of columns, cords or strands [Figure 2]. The tumor cells were polygonal, spindle- or stellate-shaped and had round to oval, bland nuclei and scant to moderate amount of deeply eosinophilic cytoplasm [Figure 2]. In the center of the lobules, abundant myxoid stroma was seen [Figure 2].
High power view showing loosely arranged tumor cells resembling chondroblasts (H&E stain, x400)
JOMFP-20-151-g003.jpg
PMC4860919_01_JOMFP-20-151-g003.jpg
F3
In addition, the cells in the center were loosely arranged with some forming small nests or clusters resembling chondroblasts [Figure 3].
Tumor cells positive for intracytoplasmic glycogen (PAS stain, x200)
JOMFP-20-151-g004.jpg
PMC4860919_01_JOMFP-20-151-g004.jpg
F4
These cells were periodic acid-Schiff [Figure 4] and vimentin [Figure 5a] positive and negative for pan-cytokeratin [Figure 5b], S-100 [Figure 5c] and epithelial membrane antigen (EMA) [Figure 5d].
Immunohistochemical analysis of extraskeletal myxoid chondrosarcoma (a) tumor cells strongly reactive to vimentin (IHC stain, x200) (b) tumor cells nonreactive to pan-cytokeratin (IHC stain, x200) (c) tumor cells nonreactive to S-100 (IHC stain, x100) (d) tumor cells nonreactive to epithelial membrane antigen (IHC stain, x100)
JOMFP-20-151-g005.jpg
PMC4860919_01_JOMFP-20-151-g005.jpg
F5
These cells were periodic acid-Schiff [Figure 4] and vimentin [Figure 5a] positive and negative for pan-cytokeratin [Figure 5b], S-100 [Figure 5c] and epithelial membrane antigen (EMA) [Figure 5d].
Myxoid matrix positive for mucin (alcian blue stain, x100)
JOMFP-20-151-g006.jpg
PMC4860919_01_JOMFP-20-151-g006.jpg
F6
The myxoid stroma was strongly positive for alcian blue [Figure 6] and mucicarmine stains [Figure 7].
Myxoid matrix positive for mucin (mucicarmine stain, x100)
JOMFP-20-151-g007.jpg
PMC4860919_01_JOMFP-20-151-g007.jpg
F7
The myxoid stroma was strongly positive for alcian blue [Figure 6] and mucicarmine stains [Figure 7].