PMC9114733_01
HPLC chromatogram of HbA1c. Abnormal peak marked by arrow.
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The chromatogram showed an abnormal peak between A1c and A0 ( Figure 1 ).
Chromatography of hemoglobin electrophoresis.
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Hemoglobin electrophoresis indicated that hemoglobin A accounted for 45.4% (normal range 96-97.6), and abnormal hemoglobin beta-chain variants accounted for 52.1% ( Figure 2 ).
Gene mutation in high-throughput sequencing.
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The thalassemia gene test suggested a rare type of beta-thalassemia (gene sequencing HBB: c.170G>A, Hb J-Bangkok (GGC->GAC at codon 56) in a beta heterozygous mutation) ( Figure 3 ), which was not one of the 17 common beta-thalassemia-related gene point mutations in Chinese individuals.