PMC9399913_01
Ovarian stimulation and blood sampling protocol in
the reported case. Routinely, blood collection is performed on day 2 on the
non-anesthetized animal.
pb-9-23-g02.jpg
PMC9399913_01_pb-9-23-g02.jpg
Ch1.F2
Three and 7 days later (i.e., on days 5 and 9 post PGF2 administration) additional blood samples were collected for progesterone monitoring with the animal being awake and non-sedated (Fig. 2).
Cross-reactivity determination of alfaxalone
measurement in the progesterone enzyme immunoassay used for analysis of
serum progesterone concentrations of common marmosets. Concentration values
at 50 % binding (50 % B0) for progesterone and alfaxalone used for
calculation of the alfaxalone cross-reactivity data are indicated.
pb-9-23-g03.jpg
PMC9399913_01_pb-9-23-g03.jpg
Ch1.F3
Moreover, the assay produces biologically valid results by demonstrating the reliable discrimination of ovarian cycle stages in the marmoset, with progesterone concentrations  10 and  10 ng mL indicating the follicular and luteal phase of the ovarian cycle, respectively (Harlow et al., 1983) (see Fig. 3 as example).