PMC9529482_01
Original magnification x40 hematoxylin and eosin stain, depicting thickened muscularis propria with an area of mucosa herniation through the muscularis propria (Rokitansky-Aschoff sinuse) and accompanying exuberant inflammatory infiltrate.
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fig1
On microscopic exam, the specimen showed chronic cholecystitis with exuberant inflammatory infiltrate (Figure 1).
Original magnification x400 hematoxylin and eosin stain, depicting a mural vessel with intraluminal large mononucleated cells with occasional folding of the nuclear membranes, open chromatin, and an inconspicuous red nucleoli, representing immature precursors.
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fig2
On careful examination, atypical cells with vesicular chromatin, folded nuclei, and inconspicuous red nucleoli were noted percolating into the gallbladder wall and lining vascular spaces (Figure 2).
Original magnification x400 CD43 immunohistochemical stain, depicting CD43 membranous staining of the intravascular immature precursors.
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fig3
These cells were positive for CD117, CD43 (Figure 3), and myeloperoxidase (Figure 4) stains and negative for CD20 and CD3 stains.
Original magnification x400 myeloperoxidase immunohistochemical stain, depicting characteristic granular cytoplasmic staining of myeloperoxidase in the intravascular immature precursors.
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fig4
These cells were positive for CD117, CD43 (Figure 3), and myeloperoxidase (Figure 4) stains and negative for CD20 and CD3 stains.