PMC4975537_01
PMC4975537_02
PMC4975537_03
PMC4975537_04
PMC4975537_05
Histopathology examination of hepatocytes. A: Liver tissue with expansion of the portal areas by infiltration of inflammatory cells. B: Trichrome stain showing bands of fibrous tissue forming portal-portal bridges (stage 4). Hepatocytes show anisonucleosis and double nuclei. Foci of confluent necrosis were seen. C: Portal area with infiltration of lymphocytes, some eosinophils, and few neutrophils resulting in interface. Foci of the infiltration of intralobular inflammatory cells were seen and the bile duct is unremarkable.
nihms805101f1.jpg
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F1
Foci of confluent necrosis were seen; the bile duct was unremarkable (Figure 1).
Sequencing result for ADK exon 6. A: Patient's sequence aligned to published template (NM_001123) using Clustal Omega software. B: Corresponding chromatogram (Chromas software version 2.4.1) for the region containing alterations. Red arrows show the altered nucleotides.
nihms805101f2.jpg
PMC4975537_05_nihms805101f2.jpg
F2
ADK deficiency. Aligned sequences and a corresponding chromatogram are shown in Figures 2A and 2B, respectively.