PMC8387073_01
A - Macroscopic feature of the partial penectomy showing a large exophytic mass with an irregular surface; B - Macroscopic cross-section of the partial penectomy showing a gray, white and congestive tissue. Scale bar = 70mm.
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The cut surface was gray and white with congestive areas (Figure 1).
Photomicrographs of the tumor. A - Pure verrucous carcinoma component (HE 100x); B - Focus of usual invasive squamous cell carcinoma (verrucous hybrid carcinoma) (HE 100x)
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The tumor front was regular, broad, and pushing with a small focus of invasion (usual SCC type), consistent with verrucous hybrid carcinoma (Figure 2).
Photomicrographs of the tumor. A - Sarcomatous component adjacent to verrucous carcinoma (HE 100x); B - Sarcomatous component (HE 200x).
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The other component was composed of atypical spindle cells arranged in vascularized bundles, mostly discohesive (Figure 3).
Photomicrographs of the tumor. A - Positive E-cadherin expression in the verrucous carcinoma component (right) and negative in the sarcomatous component (left) (100x); B - Positive vimentin expression in the sarcomatous component (100x); C - Positive p63 expression in the sarcomatous component (100x); D - Positive p53 expression in the sarcomatous component (100x).
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Vimentin and p53 were positive only in the sarcomatous component as well as loss of E-cadherin expression (Figure 4).
Photomicrographs of the tumor. PDL1 expression A - Positive expression for 22C3 (200x); B - Positive expression for SP263 (200x); C - Positive expression for SP142 (200x).
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The combined positive score (CPS) was 30% and 15% for the 22C3 and SP263 clones, respectively, and the inflammatory score (IC) was 5% for the SP142 clone (Figure 5).