PMC5763053_01
FISH and RT-PCR of the KMT2A rearrangement. (a) FISH of the KMT2A break apart signal: the green signal shows the 5' segment, and the red signal shows the 3' segment of KMT2A. FISH revealed 2 normal signals (arrowhead) and 1 additional 5' segment (arrow). Positive cells with duplication of the 5' segment of KMT2 were observed in 686 out of 1000 cells. (b) The characterization of KMT2A-MLLT10 fusion transcript at bone marrow by RT-PCR. 1: ladder; 2: normal control; 3: at diagnosis; 4: after induction therapy 1; 5: after induction therapy 2; 6: after intensification 1; 7: after intensification 2; 8 and 9: after intensification 3.
CRIPE2017-6257494.001.jpg
PMC5763053_01_CRIPE2017-6257494.001.jpg
fig1
Color, Break Apart Rearrangement Probe, Abbott Laboratories, IL, USA) was used to characterize the partial 5' duplication and 2 other normal KMT2A alleles (Figure 1). Cell culture for chromosomal analysis failed. MLLT10 breakpoint was located on exon 14. Minimal residual disease (MRD) by reverse transcription-polymerase chain reaction (RT-PCR) was assessed from diagnosis as shown in Figure 1.