PMC3010644_01
Horizontal (a) and axial (b) enhanced computed tomography (CT) showed an inhomogeneously enhancing mass that filled the left nasal cavity. A low-density lesion with metal-dense spots in the left maxillary sinus, indicating mycetoma, was also observed. Bone window images ((c), (d)) also showed a new bone formation in the tumor. Black arrows indicate a new bone formation.
IJOL2010-154948.001.jpg
PMC3010644_01_IJOL2010-154948.001.jpg
fig1
The tumor grew expansively and showed marked osteogenesis (Figure 1).
Horizontal (a) and axial (b) magnetic resonance imaging (MRI) showed a nasal tumor in the left nasal cavity with a hypointense signal on T1-weighted images, and a moderately hyperintense signals on T2-weighted images. This signal was different from that of the left maxillary sinus lesions.
IJOL2010-154948.002.jpg
PMC3010644_01_IJOL2010-154948.002.jpg
fig2
T1-weighted images, and a moderately hyperintense signals on T2-weighted images (Figure 2). The signal of the nasal tumor was distinctly different from that of the left maxillary sinus lesions.
Macroscopic (a) and microscopic (x100 magnification) (b) features of the excised tumor. The spindle cells are arranged in a patternless fashion, and new bone formation consisting of randomly organized trabeculae lined by osteoblasts was observed in the tumor. black arrows and *asterisk: bone tissue.
IJOL2010-154948.003.jpg
PMC3010644_01_IJOL2010-154948.003.jpg
fig3
The tumor was thus successfully removed in one piece with the surgical margin free of disease (Figure 3(a)).  Histopathologically, a patternless proliferation of spindle cells and new bone formation consisting of randomly organized trabeculae lined by osteoblasts were observed in the tumor (Figure 3(b)).
Immunohistochemistry for bone morphogenic protein (BMP; x100 magnification). (a) BMP-2, (b) BMP-4, and (c) BMP-7. The tumor cells stained positive for BMP-4 and negative for BMP-2 and BMP-7. *asterisk: bone tissue.
IJOL2010-154948.004.jpg
PMC3010644_01_IJOL2010-154948.004.jpg
fig4
IgG (Vector Laboratories, Burlingame, CA). After rinsing, sections were incubated with ABC reagent (Vector Laboratories) and developed in 0.05% 3,3'-diaminobenzidine-0.01% H2O2 substrate medium in 0.1 M phosphate buffer. Interestingly, the tumor cells stained positive for BMP-4 and negative for BMP-2 and BMP-7 (Figure 4).
Western blot analysis for BMP. The tumor tissue expressed BMP-4 but not BMP-2 and -7.
IJOL2010-154948.005.jpg
PMC3010644_01_IJOL2010-154948.005.jpg
fig5
The result showed the expression of BMP-4, but not BMP-2 and -7, in the similar manner to the result of immunohistochemistry (Figure 5). 