PMC7494188_01
Case timeline. UTI, urinary tract infection; MR, magnetic resonance; TUMT, transurethral microwave therapy, CT, computed tomography; PET/CT, positron emission tomography with CT.
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F1
He had a medical history of prostatic hypertrophy complicated with recurrent urinary tract infections (Fig. 1).
PMC7494188_02
F. sanguinis
 on Brucella blood agar.
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F2
However, a clear double zone was observed and the colonies close to the metronidazole disk were slightly smaller, a bit greyer and flatter than the colonies in the periphery (Fig. 2).
(a) Alignment of the partial 16S rRNA gene sequence from the case isolate of 
F. sanguinis
 with 
F. sanguinis
 strain CCUG 47711 chromosome, complete genome (sequence ID: CP027226). There are two mismatches at position 202 (G to R=A or G) and at position 439 (A to R) resulting in a match of 99.5 %. (b) Alignment of the sequence obtained from the mixed sequence from the case isolate using the Pathogenomix Ripseq software and database, of 
M. indoligenes
 with 
M. indoligenes
 strain MDA2477 (sequence ID: AF527773). There are several mismatches and gaps, resulting in a match of 95.4 %.
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F3
F. sanguinis  with a score of 99.5 was suggested (442 bp sequence, sequence ID: CP027226, coverage of 100%) using the Basic Local Alignment Search Tool (blast) (Fig. 3). For the colonies in the periphery, a mixed chromatogram was found, suggesting DNA from more than one species. The DNA sequence of the  Lachnospiraceae  species (sequence ID: NBBL01000005) was further investigated using blast.  M. indoligenes  was identified with a score of 95.4 (sequence ID: AF527773) (Fig. 3), which is a very high score given that the data originated from a mixed sequence.
PMC7494188_03