PMC4150342_01
(a) Patient with right orbital mass and chest wall mass. (b) Photograph of patient after chemotherapy. (c) Cytology of orbital mass (H and E x100)
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F1
The case we present here is a 14-year-old female child who presented with low backache since 1 year followed by right eye proptosis for 4 months and swelling over right side chest wall since 3 months [Figure 1a]. Fine needle aspiration smears from orbital mass were highly cellular showing cells arranged both in clusters and singly dispersed [Figure 1c].
(a) Photomicrograph showing small round cells in tissue section (H and E x100). (b) Under higher magnification, (H and E x400). (c) Diffuse and strong positivity with CD99 (IHC, x400). (d) Reverse transcription-polymerase chain reaction analysis of Ewing's sarcoma
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F2
Histologically the tumor cells were arranged in irregular nests separated by dense fibrous stroma [Figure 2a]. The cells were 1-2 times larger than small lymphocytes with scant eosinophilic or clear cytoplasm, centrally placed round nuclei with finely distributed chromatin and inconspicuous nucleoli [Figure 2b]. The paraffin block was subjected to CD99 immunohistochemical marker and was strongly and diffusely positive [Figure 2c]. The EWS-FLI-1 product was type 1 fusion (330 bp) [Figure 2d].