PMC3336230_01
Riskettsial organisms in recently drained CSF; (a) stained with anti-rickettsial antiserum and Alexa Fluor 488 goat anti-rabbit immunoglobulin; (b) the same field of view shows the organisms DNA stained with DAPI in the same location.
CRIM.ID2011-469194.001.jpg
PMC3336230_01_CRIM.ID2011-469194.001.jpg
fig1
As mounting medium VECTASHIELD with DAPI (4', 6-diamidino-2-phenylindole) (Vector Laboratories, Burlingame, USA) was used according to the manufacturers instruction, showing the same fluorescent organisms positive for bacterial DNA (Figures 1(a) and 1(b)).