PMC4228273_01
Cytomorphologic and cytogenetic results. (A) A representative cytomorphologic bone marrow field, showing blasts with Auer rods (arrow) and azurophilic inclusions. (B) A representative metaphase cell demonstrating normal chromosomes 15 and 17, and more than 20 double minutes (red arrowheads). (C) Metaphase FISH of this patient with the ON MYC(green)/IGH(red) t(8;14) Fusion Probe (Kreatech), showing MYC-positive dmins (green), and both chromosomes 14 (arrow); in this cell it was not possible to indicate the normal chromosome 8 due to the high number of dmins (D) Metaphase FISH with a BAC-probe RP1-80K22 (base pair position 128,667,455-128,814,588) for MYC gene (red) and a flanking BAC-probe RP11-125A17 (base pair position 128,865,417-129,036,660; green), demonstrating multiple copies of dmins (red-green signals). Only one chromosome 8 (arrow) contains the MYC gene region.
13039_2014_67_Fig1_HTML.jpg
PMC4228273_01_13039_2014_67_Fig1_HTML.jpg
Fig1
The peripheral blood smear revealed 37 x 109/l blasts, with Auer rods, but also 78 x 109/l promyelocytes, with some faggot cells, resembling typical APL (Figure 1A).