PMC4966718_01
Cytogenetic, FISH and Immunophenotype results 1a G-banded karyotype from the sideline 46,XY,del(9)(p22)t(9;22)(q34;q11),del(20)(q13). 1b Metaphase FISH image showing BCR-ABL1 fusion gene at minor breakpoint BCR. Green BCR, red ABL1. 1c G-banded karyotype showing t(9;22)(q34;q11) as a sole chromosome alteration. 1d Metaphase FISH image showing BCR-ABL1 fusion gene at minor breakpoint BCR. LSI BCR/ABL1 dual color translocation probe (Abbott) was used. Green: BCR, Red: ABL1. 2a Immunophenotype of ALL with atypical e1a3 translocation. Presence of 90 % of blasts with lymphoblastic phenotype B: CD19+, CD10+, CD34+, DR+, CD20+, CD22-, CD33+, cytoplasmic IgM negative and positive alpha CD79. 2b Immunophenotype of ALL with atypical e1a3 translocation. Presence of 80 % of blasts with lymphoblastic phenotype B: CD19+, CD10+, CD34+, DR+, CD20+, CD22-, cytoplasmic IgM negative, positive alpha CD79 and dim expression of CD33
40164_2016_49_Fig1_HTML.jpg
PMC4966718_02_40164_2016_49_Fig1_HTML.jpg
Fig1
FISH analysis confirmed the presence of BCR-ABL1 fusion gene (Fig. 1).
DNA sequencing of BCR-ABL e1a3 atypical amplification band purified from both patients
40164_2016_49_Fig2_HTML.jpg
PMC4966718_02_40164_2016_49_Fig2_HTML.jpg
Fig2
We identified an atypical e1a3 variant after a two round PCR amplification by DNA sequencing (Fig. 2).
PMC4966718_02
Cytogenetic, FISH and Immunophenotype results 1a G-banded karyotype from the sideline 46,XY,del(9)(p22)t(9;22)(q34;q11),del(20)(q13). 1b Metaphase FISH image showing BCR-ABL1 fusion gene at minor breakpoint BCR. Green BCR, red ABL1. 1c G-banded karyotype showing t(9;22)(q34;q11) as a sole chromosome alteration. 1d Metaphase FISH image showing BCR-ABL1 fusion gene at minor breakpoint BCR. LSI BCR/ABL1 dual color translocation probe (Abbott) was used. Green: BCR, Red: ABL1. 2a Immunophenotype of ALL with atypical e1a3 translocation. Presence of 90 % of blasts with lymphoblastic phenotype B: CD19+, CD10+, CD34+, DR+, CD20+, CD22-, CD33+, cytoplasmic IgM negative and positive alpha CD79. 2b Immunophenotype of ALL with atypical e1a3 translocation. Presence of 80 % of blasts with lymphoblastic phenotype B: CD19+, CD10+, CD34+, DR+, CD20+, CD22-, cytoplasmic IgM negative, positive alpha CD79 and dim expression of CD33
40164_2016_49_Fig1_HTML.jpg
PMC4966718_02_40164_2016_49_Fig1_HTML.jpg
Fig1
FISH analysis confirmed the presence of BCR-ABL1 fusion gene (Fig. 1).
DNA sequencing of BCR-ABL e1a3 atypical amplification band purified from both patients
40164_2016_49_Fig2_HTML.jpg
PMC4966718_02_40164_2016_49_Fig2_HTML.jpg
Fig2
We identified an atypical e1a3 variant after a two round PCR amplification by DNA sequencing (Fig. 2).