PMC5283969_01
The clinical course of the patient. AZA: azacitidine, WT1: Wilms' Tumor 1, dROM: derivatives of reactive oxygen metabolite, 8-OHdG: 8-hydroxy- 2'-deoxyguanosine, FER: ferritin, ( ): red blood cell transfusion 2 units. Normal ranges: serum dROM; <300 U. CARR, urinary 8-OHdG; <15.7 ng/mg creatinine ferritin; 18.6-261 ng/dL, lactate dehydrogenase (LDH); 120-240 IU/L.
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Tumor 1 (WT1) gene in the peripheral blood throughout the course of treatment (Fig. 1). The level of WT1 was 14,000 copies/mug RNA before the first course of azacitidine treatment, which was reduced markedly to 400 copies/mug RNA after the second course.
The relationship among the derivatives of reactive oxygen metabolite (dROM), 8-hydroxy-2'-deoxyguanosine (8-OHdG), ferritin (FER), and Wilms' Tumor 1 (WT1). The correlation between (a) dROM and WT1, (b) dROM and FER, (c) 8-OHdG and WT1, (d) 8-OHdG and FER, (e) 8-OHdG and dROM, (f) dROM and C reactive protein (CRP), and (f) 8-OHdG and CRP.
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The serum dROM levels correlated significantly with WT1 (r=0.80, p=0.03) and ferritin (r=0.85, p=0.002) (Fig. 2a and b). In addition, the urinary 8-OHdG levels also correlated significantly with WT1 (r=0.96, p=0.002) and ferritin (r=0.92, p=0.000) (Fig. 2c and d). The serum dROM levels also correlated significantly with 8-OHdG (r=0.69, p=0.01) (Fig. 2e). On analyzing the correlation among C reactive protein (CRP), dROM, and 8-OHdG, CRP did not correlate with dROM (r=0.45, p=0.138) or 8-OHdG (r=-0.21, p=0.534) (Fig. 2f and g).