PMC8524739_01
A) Partial G-banding karyotype of homologues No20 showing del(20) (q11.2q13.1). B) FISH examination of bone marrow cells - (a) granulocyte with one red (PTPRT gene) and one green signal (MAPRE1 gene)(clone No2); (b) mononuclear cell with one red and three green signals (clone No3); (c) mononuclear cell with one red and two green signals (clone No1); (d) mononuclear cell with normal FISH pattern - two red and two green signals. C) FISH examination of purified CD138+ bone marrow cells showing one red and three green signals (clone No3).
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PMC8524739_01_gr1.jpg
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Conventional banding analysis of bone marrow cells showed karyotype of 46,XY,del(20)(q11.2q13.1)[5] / 46,XY[25] (Fig. 1A). The 20q- anomaly from the first and third clone was observed only in the mononuclear cells, while the anomalies of the second clone in the mononuclear cells as well as in granulocytes (metamyelocytes, bands and segments) (Fig. 1B). Interphase FISH examination on purified CD138+ cells revealed that the myeloma cells carried the deletion of the PTPRT gene and the duplication of the MAPRE1 gene (Fig. 1C).