PMC10340522_01
(A) After centrifugation of bronchoalveolar lavage fluid, gram staining of the precipitate revealed gram-positive and 'bottle-shaped' budding yeast cells (black arrows indicated). (B) Bronchoalveolar lavage fluid staining with gomori methenamine silver showed dark brown and budding spores (black arrows indicated). (C) Gram staining of the colonies showed 'bottle-shaped' budding yeast cells, with a collarette-like thickening seen at the junction between the budding and the mother spores.
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After centrifugation of bronchoalveolar lavage fluid (BALF), gram staining and Gomori methenamine silver (GMS) staining revealed some bottle-shaped and budding yeast cells ( Figures 1A, B ). Gram staining of the colonies showed a culster of budding yeast cells, with a collarette-like structure seen at the junction between the budding and the mother spore ( Figure 1C ).
(A) Dust-like colonies were found in bronchoalveolar lavage fluid cultured on sabouraud dextrose agar plate for 3 days at 35 C. (B) Small creamy and fragile colonies were observed on the sabouraud dextrose agar plate after 5 days at 35 C. (C) After 3 days of incubation on sabouraud dextrose agar covered with sterile olive oil at 35 C, the morphology of colonies was cheesy, raised, smooth or rough. (D) The colonies appeared pink on CHROMagar  Candida with sterile olive oil supplementation after 3 days at 35 C.
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Dust-like colonies grew on the SDA after 3 days of incubation ( Figure 2A ) but not on blood agar. After 5 days of culture, small creamy and fragile colonies were observed on SDA plate ( Figure 2B ). After 3 days of incubation, the morphology of colonies on the SDA covered with olive oil ( Figure 2C ) were cheese-colored, raised, smooth or rough, and pink on CHROMagar  Candida covered with olive oil ( Figure 2D ).