PMC5584765_01
Peripheral blood smears (A) and electron microscopic examination (B) show atypical lymphocytes with partially hairy projections and abundant irregularly shaped cytoplasms. No ribosome-lamella complex was found.
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Peripheral blood smears and electron microscopic examination found that these atypical lymphocytes had irregularly shaped abundant cytoplasms with partially hairy projections (Figure 1).
Detection of immunoglobulin heavy chain (IGH) gene recombinations using multiplex polymerase chain reaction (PCR) using a tube A primers set (VH FR1) and one consensus primer (JH) in accordance with the BIOMED-2 protocol. Many different IGH PCR products without a monoclonal peak were detected.
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Multiplex PCR analysis using the three sets of VH primers and one JH consensus primer as specified for the BIOMED-2 primer sets showed no monoclonal peak of IGH gene recombination (Figure 2).