PMC1200253_01
Slit lamp biomicroscopy of cornea of case 1 in diffuse illumination with central mid to deep stromal infiltrate and surrounding stromal edema.
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F1
Trichrome stain (figure 7), 1% acid fast stain (figure 8) and variably with GMS.
PMC1200253_02
Slit lamp biomicroscopy of cornea of case 3 in diffuse illumination shows a large stromal infiltrate.
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F2
He was on regular follow-up and seven months later a recurrence was seen with a stromal infiltrate and the presence of endothelial exudates (figure 2).
PMC1200253_03
Slit lamp biomicroscopy of cornea of case 4 in diffuse illumination shows two discreet stromal infiltrates.
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F3
Left eye lids were edematous, conjunctiva was congested, cornea showed the presence of an anterior mid stromal infiltrate in nasal paracentral area with overlying epithelial defect of 1.5 x 1.5 mm (figure 3).
PMC1200253_04
Slit lamp biomicroscopy of cornea of case 1 in diffuse illumination with central mid to deep stromal infiltrate and surrounding stromal edema.
1471-2415-5-19-1.jpg
PMC1200253_04_1471-2415-5-19-1.jpg
F1
Trichrome stain (figure 7), 1% acid fast stain (figure 8) and variably with GMS.
Gram stain of corneal scraping showing gram positive oval microsporidial spores (x500, 4a).
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F4
The Gram (figure 4) and Giemsa stained smears revealed variable staining of the parasites.
1% acid fast stain of corneal scraping showing acid fast positive oval microsporidial spores (x500, 4b).
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F5
The parasite was acid fast and appeared as red oval structure on smears stained with 1% acid fast stain (figure 5).
Section showing intact epithelium, with deep stromal infiltrates (arrow) (Hematoxylin and Eosin stain, x20).
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F6
There was moderate to severe stromal inflammation, at places forming micro abscess (fig 6).
Section of corneal button which shows oval bluish purple spores of microsporidia with a dark band (modified grams chromotrope stain, x1000).
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F7
Trichrome stain (figure 7), 1% acid fast stain (figure 8) and variably with GMS.
Section of corneal button in which the spores appear red with darkly stained band at the tip (1% acid-fast stain, x1000).
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F8
Trichrome stain (figure 7), 1% acid fast stain (figure 8) and variably with GMS.
Section of corneal button of case 4 showing marked thinning in the central stroma (descematocele, arrow).
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F9
The case with corneal scar and a descematocele showed a corneal scar with marked thinning in the central stroma (figure 9).
Section of corneal button of case 4 showing the presence of oval, spores of microsporidia in the stroma, unaccompanied by inflammatory infiltrates (PAS stain, x500).
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F10
There was no inflammatory infiltrate, but the stroma showed oval, spores of microsporidia in all section (figure 10).
The electron microscopic examination of the corneal button revealing macrophage with three spores.
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F11
The semithin sections of these tissues showed many viable, mature, immature, and degenerated spores, many of them being intracellular. The electron microscopic examination revealed an electron lucent thick capsule with a single nucleus and 11 - 13 tubules in the cytoplasm (figure 11 with inset as figure 12).
Inset shows an electron lucent thick capsule with a single nucleus and 11 - 13 tubules in the cytoplasm.
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F12
The semithin sections of these tissues showed many viable, mature, immature, and degenerated spores, many of them being intracellular. The electron microscopic examination revealed an electron lucent thick capsule with a single nucleus and 11 - 13 tubules in the cytoplasm (figure 11 with inset as figure 12).