PMC9379092_01
(A,B) The gross picture of the broad ligament myoma and incised specimen. The mass was found to have originated from the right broad ligament. The cervix was pointed by the tip of the Kelly clamp (A). The leiomyoma was throughout solid and presented with only a small portion of degeneration (B).
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F1
During surgery, the pelvic mass was found to have originated from the right broad ligament, while the right ovary had a 3 cm dermoid cyst (Figures 1A,B).
(A-D): Immunohistochemical staining revealed excessive Epo and Epo-R expression of the broad ligament myoma. Immunochemical staining showed diffuse (>50%) Epo expression with moderate to strong staining intensity over the myomatous cells (A, 40x), whereas the endothelial cells were negatively stained (B, 200x). A diffuse (>50%) Epo-R expression with moderate staining intensity was found over the myomatous cells (C, 40x). The endothelial cells also presented with diffuse, moderate to weak staining intensity (D, 200x). EPO, erythropoietin; Epo-R, erythropoietin receptor.
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F2
Biosciences) and erythropoietin receptor (Epo-R) (rabbit polyclonal antibody, A2917, ABclonal Science). A diffuse Epo expression with moderate to strong staining intensity appeared over the myomatous tissue, but the staining was negative for the endothelial cells (Figures 2A,B). Both the myomatous and endothelial cells were positive for Epo-R immunostaining (Figures 2C,D).
H&E staining showing wide distribution of mature vessels within the myomatous tissue. Under H&E stain (40x), the myomatous tissue presented with a vast distribution of mature vessels. The vessels bear the characteristics of dilated veins and arteries with smooth muscle layers and a luminal space of >50 microm in minimum diameter. H&E, hematoxylin and eosin.
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F3
We also noticed an extensive distribution of mature blood vessels in the specimen under hematoxylin and eosin (H&E) stain (Figure 3).