PMC8720759_01
Tumor images of patients A and B. (A) Patient A, right inguinal squamous cell carcinoma 2 weeks after first cycle of experimental immune therapy. Tumor surface became smooth and epithelial margins symmetric compared to original tumor state. (B) 11 weeks after second experimental treatment, the patient returned for the last cycle of intratumoral vaccinations and topical imiquimod therapy. The tumor measured 2 cm3. (C) Patient B, hematoxylin and eosin stain of recurrent cervical cancer showing areas of invasive focally keratinizing, moderately differentiated squamous carcinoma involving entire thickness of the stroma (x20 magnification, scale 100 microm). (D) Patient B, tumor identified and biopsied 3-4 weeks after completion of chemo-radiation for stage 3B squamous cell cancer of the cervix, pelvic exam, PET/CT, and biopsies confirmed radiation-refractory, recurrent disease. (E) Radical hysterectomy and bilateral salpingo-oophorectomy following failed curative chemo-radiation (8,500 cGy total to point A) followed by two cycles of intratumoral vaccinations and imiquimod. No residual squamous cell carcinoma on pathological evaluation and negative HPV-HR testing of the cervix. Vaginal cuff ThinPrep  6 weeks post-operatively was negative for dysplasia/malignancy and HPV-HR DNA.
pore-27-1609922-g001.jpg
PMC8720759_03_pore-27-1609922-g001.jpg
F1
The patient was a 41-year-old Caucasian female, diagnosed with stage IIIB, HPV-HR positive, moderately differentiated squamous cell carcinoma of the cervix (Figure 1C). Exam found a 3-4 cm recurrent, grade 1, squamous cell carcinoma, (Figure 1D). Therefore, in light of these new findings, the planned pelvic exenteration was aborted and post-radiation, radical hysterectomy with upper vaginectomy, bilateral salpingo-oopherctomy and pelvic and para-aortic lymphadenectomy was performed (Figure 1E).
PMC8720759_02
Tumor images of patients A and B. (A) Patient A, right inguinal squamous cell carcinoma 2 weeks after first cycle of experimental immune therapy. Tumor surface became smooth and epithelial margins symmetric compared to original tumor state. (B) 11 weeks after second experimental treatment, the patient returned for the last cycle of intratumoral vaccinations and topical imiquimod therapy. The tumor measured 2 cm3. (C) Patient B, hematoxylin and eosin stain of recurrent cervical cancer showing areas of invasive focally keratinizing, moderately differentiated squamous carcinoma involving entire thickness of the stroma (x20 magnification, scale 100 microm). (D) Patient B, tumor identified and biopsied 3-4 weeks after completion of chemo-radiation for stage 3B squamous cell cancer of the cervix, pelvic exam, PET/CT, and biopsies confirmed radiation-refractory, recurrent disease. (E) Radical hysterectomy and bilateral salpingo-oophorectomy following failed curative chemo-radiation (8,500 cGy total to point A) followed by two cycles of intratumoral vaccinations and imiquimod. No residual squamous cell carcinoma on pathological evaluation and negative HPV-HR testing of the cervix. Vaginal cuff ThinPrep  6 weeks post-operatively was negative for dysplasia/malignancy and HPV-HR DNA.
pore-27-1609922-g001.jpg
PMC8720759_03_pore-27-1609922-g001.jpg
F1
The patient was a 41-year-old Caucasian female, diagnosed with stage IIIB, HPV-HR positive, moderately differentiated squamous cell carcinoma of the cervix (Figure 1C). Exam found a 3-4 cm recurrent, grade 1, squamous cell carcinoma, (Figure 1D). Therefore, in light of these new findings, the planned pelvic exenteration was aborted and post-radiation, radical hysterectomy with upper vaginectomy, bilateral salpingo-oopherctomy and pelvic and para-aortic lymphadenectomy was performed (Figure 1E).
PMC8720759_03
Tumor images of patients A and B. (A) Patient A, right inguinal squamous cell carcinoma 2 weeks after first cycle of experimental immune therapy. Tumor surface became smooth and epithelial margins symmetric compared to original tumor state. (B) 11 weeks after second experimental treatment, the patient returned for the last cycle of intratumoral vaccinations and topical imiquimod therapy. The tumor measured 2 cm3. (C) Patient B, hematoxylin and eosin stain of recurrent cervical cancer showing areas of invasive focally keratinizing, moderately differentiated squamous carcinoma involving entire thickness of the stroma (x20 magnification, scale 100 microm). (D) Patient B, tumor identified and biopsied 3-4 weeks after completion of chemo-radiation for stage 3B squamous cell cancer of the cervix, pelvic exam, PET/CT, and biopsies confirmed radiation-refractory, recurrent disease. (E) Radical hysterectomy and bilateral salpingo-oophorectomy following failed curative chemo-radiation (8,500 cGy total to point A) followed by two cycles of intratumoral vaccinations and imiquimod. No residual squamous cell carcinoma on pathological evaluation and negative HPV-HR testing of the cervix. Vaginal cuff ThinPrep  6 weeks post-operatively was negative for dysplasia/malignancy and HPV-HR DNA.
pore-27-1609922-g001.jpg
PMC8720759_03_pore-27-1609922-g001.jpg
F1
The patient was a 41-year-old Caucasian female, diagnosed with stage IIIB, HPV-HR positive, moderately differentiated squamous cell carcinoma of the cervix (Figure 1C). Exam found a 3-4 cm recurrent, grade 1, squamous cell carcinoma, (Figure 1D). Therefore, in light of these new findings, the planned pelvic exenteration was aborted and post-radiation, radical hysterectomy with upper vaginectomy, bilateral salpingo-oopherctomy and pelvic and para-aortic lymphadenectomy was performed (Figure 1E).