PMC9556986_01
Magnetic resonance imaging of the thoracolumbar spine. T1-weighted image (A) and T2-weighted STIR image (B) of the destructed thoracolumbar spine in sagittal orientation, white arrows indicate destruction of vertebrae; Cystic mass (white asterisk) is found in the T2-weighted image in cross-sectional orientation (C).
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F1
On initial admission one year ago, Magnetic resonance imaging (MRI) suggested destruction of the thoracic vertebrae (T11 and T12) (Figures 1A,B), and computed tomography (CT) scans revealed destruction of the right 12th rib (Figure 2). MRI showed multiple cystic masses in the craniocervical junction region and effusion around the lumbar vertebrae (Figure 1C). Nothing special was noted in the abdominal contrast enhanced CT of the patient (Supplementary Figure S1).
Computed tomography scans of the thoracolumbar spine. Local magnification of cross-sectional (A), coronal (B) and sagittal images. White arrows indicate destruction of vertebrae (A,C), and destruction of the 12th rib (B).
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F2
On initial admission one year ago, Magnetic resonance imaging (MRI) suggested destruction of the thoracic vertebrae (T11 and T12) (Figures 1A,B), and computed tomography (CT) scans revealed destruction of the right 12th rib (Figure 2). Table S1). The whole timeline of the patients was displayed in Supplementary Figure S2.
Investigation and validation results of metagenomic next-generation sequencing. Mapping of the detected reads from the specimen cover the genome of Echinococcus multilocularis (A); Echinococcus multilocularis was identified as the most predominant pathogen constituting 99.74% (3,938 reads) of the total sequence reads (B).
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F3
Among them, the detected reads from the specimen mapped well with the Echinococcus multilocularis genome (Figure 3A). As for the constitution, Echinococcus multilocularis was identified as the most predominant pathogen taking up to 99.74% (3,938 reads) of the total sequence reads (Figure 3B).
The Sanger sequencing results are consistent with sequence alignment of the tapeworm cox1 gene. Gel electrophoresis of PCR products (A); Sanger sequencing results (B).
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F4
The sequencing results are consistent with the sequence alignment of the tapeworm cox1 gene, which further confirmed the mNGS results (Figure 4).