PMC6637709_01
Preoperative CBCT showing well-defined unilocular radiolucency associated with an impacted right third molar extending to the distal root of the second molar.
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Clinical Hospital of the Federal University of Goias, Goiania, Goias, Brazil, for evaluation of an asymptomatic radiolucent lesion in the posterior mandible region. Cone-beam computed tomography (CBCT) scan showed a well-defined unilocular radiolucency associated with an impacted right third molar, extending to the distal root of the second molar, measuring 17 x 12.5 mm (Figure 1).
Microscopic features of the GOC. (a) Cystic cavity lined by nonkeratinized stratified squamous epithelium of varying thickness. (b) Duct-like structures observed in the cystic lining. (c) Presence of numerous goblet cells. (d) Note the presence of eosinophilic cuboidal cells. Slides stained with haematoxylin and eosin. Original magnifications: 50x and 400x.
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The histopathological examination revealed cyst wall lining by nonkeratinized stratified squamous epithelium with varied thickness (Figure 2(a)). Duct-like structures surrounded by cuboidal cells and numerous mucous cells were also identified (Figures 2(b) and 2(c)). The superficial layer of the epithelium showed columnar ciliated and eosinophilic cuboidal cells, also called "hobnail cells" (Figure 2(d)).
Special stains for GOC. (a, b) Periodic acid-Schiff- (PAS-) and periodic acid-Schiff diastase- (PAS-D-) positive goblet cells showing glycogen-rich cells. (c) Note the glandular-like structure stained by PAS-D. Mucin-secreting cells were also identified.
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Glycogen-rich and mucin-secreting cells were highlighted by periodic acid-Schiff (PAS), periodic acid-Schiff diastase (PAS-D) (Figures 3(a)-3(c)), and mucicarmine staining (Figure 3(d)).
Patient's orthopantomogram (OPG) showing bone healing after a 2-year follow-up.
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The postoperative orthopantomogram (OPG) revealed no recurrence one year postsurgery (Figure 4).