PMC7493633_01
(A) Chest CT at day 5 showing bilateral peripheral ground-glass opacities. (B) Chest CT at day 17 showing opacities at different locations without significant reduction.
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F1
A chest CT was performed, which showed bilateral peripheral ground-glass opacities (Figure 1A). TID, follow-up chest CT at day 16 (Figure 1B) showed no significant improvement with multiple ground-glass opacities at different locations.
Analysis of SARS-CoV-2 specific T cells. Whole blood at day 19 was stimulated with overlapping peptide pools of the SARS-CoV-2 spike glycoprotein (divided in the N-terminal and C-terminal part, Spike N, and Spike C, respectively), the nucleocapsid protein (NCAP), the membrane protein (VME1), and the envelope small membrane protein (VEMP) for 6 h and antigen-specific CD4 and CD8 T cells were quantified based on the induction of CD69 and IFNgamma. Stimulations with the solvent (DMSO), with Staphylococcus aureus Enterotoxin B (SEB), and with cytomegalovirus (CMV) antigens were carried out as negative and positive controls, respectively. Percentages in each dotplot refer to the percentages of specifically stimulated CD4 or CD8 T cells.
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F2
SARS-CoV-2 specific T cells primarily reactive toward peptide pools from the viral spike glycoprotein, nucleocapsid protein, and membrane protein (VME1, Figure 2). Specific cells with immediate effector function were mainly found among CD4 T cells (Figure 2).