PMC4122198_01
Definitive histological examination showing a carcinosarcoma with carcinomatous and sarcomatous components. Immunohistochemistry with monoclonal antibodies (anticytokeratin CK7, CK 8-18, and CK 5-6) for actin, P63, vimentin, S100, desmin, and EMA (epithelial membrane antigen) has been performed. The tumour showed strong positivity for vimentin, EMA, and focal elements for CK 8-18. (a) Hematoxylin-eosin (20x). "In situ" squamous cell carcinoma with cells infiltrating the subepithelial corium. Lower right: undifferentiated spindle cell carcinoma cells. (b) Hematoxylin-eosin (20x). "In situ" squamous cell carcinoma with atypical cells at the basal layers, a sarcomatous component, and hemosiderin deposits. (c) EMA (20x). Immunohistochemistry: carcinomatous cells with membrane positivity for EMA. (d) CK 8-18 (40x): focal positivity for cytokeratin 8-18.
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