PMC5336392_01
Bone marrow biopsy (H & E), 20% cellularity (magnification x 100).
can-11-720fig1.jpg
PMC5336392_01_can-11-720fig1.jpg
figure1
Staining for PNCA was less than 1% and there was no evidence of fibrosis or lymphoid follicular formation (Figure 1).
Low power biopsy ganglia (x 10).
can-11-720fig2.jpg
PMC5336392_01_can-11-720fig2.jpg
figure2
There was 70% of the lymph node parenchyma being replaced with an infiltration of morphologically mature plasma cells as well as loss of the normal architecture (Figure 2, 3).
Infiltration by plasma cells (x 400).
can-11-720fig3.jpg
PMC5336392_01_can-11-720fig3.jpg
figure3
There was 70% of the lymph node parenchyma being replaced with an infiltration of morphologically mature plasma cells as well as loss of the normal architecture (Figure 2, 3).
Plasma cells CD38 (+) (x 10).
can-11-720fig4.jpg
PMC5336392_01_can-11-720fig4.jpg
figure4
Plasma cells CD19 (+) (x 10).
can-11-720fig5.jpg
PMC5336392_01_can-11-720fig5.jpg
figure5
Kappa expression (x 400).
can-11-720fig6.jpg
PMC5336392_01_can-11-720fig6.jpg
figure6
Lambda expression (x 600).
can-11-720fig7.jpg
PMC5336392_01_can-11-720fig7.jpg
figure7
Bone marrow biopsy showing hypercellular features and infiltration by plasma cells (x 400).
can-11-720fig8.jpg
PMC5336392_01_can-11-720fig8.jpg
figure8
The bone marrow biopsy (Figure 8) showed a variable cellularity occupying between 10-90% of the intertrabecular spaces with over 70% being plasma cells, diffusely infiltrated between a few myeloid precursors.
Bone marrow biopsy stained for kappa (x 400).
can-11-720fig9.jpg
PMC5336392_01_can-11-720fig9.jpg
figure9
Immunocytochemistry showed a lambda:kappa ratio of 9:1 (Figure 9, 10), the plasma cells being negative for CD20 and positive for CD19, CD38, and CD138, and 20% of plasma cells being positive for Bcl-2.
Bone marrow stained for lambda (x 1000).
can-11-720fig10.jpg
PMC5336392_01_can-11-720fig10.jpg
figure10
Immunocytochemistry showed a lambda:kappa ratio of 9:1 (Figure 9, 10), the plasma cells being negative for CD20 and positive for CD19, CD38, and CD138, and 20% of plasma cells being positive for Bcl-2.
Flow cytometry of bone marrow showing CD56 (-) CD19 (+) phenotype.
can-11-720fig11.jpg
PMC5336392_01_can-11-720fig11.jpg
figure11
The cells were CD56, CD117, CD20, CD22, and HLA-DR negative (Figure 11).