PMC3424686_01
(a) Cell block of FNA material showing a striking nuclear pleomorphism as well as a high mitotic index with irregular mitoses and numerous apoptotic bodies (H and E stain, magnification x200). (b) Higher magnification of the a, with particular emphasis on the details noted above (H and E stain, magnification x400)
CJ-9-16-g001.jpg
PMC3424686_01_CJ-9-16-g001.jpg
F1
A high mitotic activity, with abnormal mitoses was present, and a large number of apoptotic bodies as well as nuclear debris were additionally seen [Figures 1a and 1b].
DNA quality control by Specimen Control Size ladder mix supplied with the kit. Samples at positions 1, 5, 6, 7, and 8 are controls supplied with the kit. Samples at positions 2, 3, and 4 are from patients. Sample 3 is the requested sample. Samples 2 and 4 have bad quality of DNA. M: PhiX174 / HaeIII digest ladder (New England Biolabs).
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F3
The DNA was extracted and amplified by primers that target the conserved framework of the variable (V) regions, the conserved joining (J) regions, as well as the diversity and joining regions, according to the manufacturer's instructions. The DNA quality was tested by the Specimen Control Size Ladder Mix, which contains multiple oligonucleotides targeting the housekeeping genes [supplied with the Kit, Figure 3].
Identification of Clonal Immunoglobulin Heavy Chain Gene Rearrangements in non-denaturing polyacrylamide electrophoresis gels (PAGE). Positions 1 and 8 polyclonal controls, positions 2 -4, 9 - 11 samples, and positions 5 - 8, 12 - 16 clonal controls. Requested sample at positions 3 and 10.
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PMC3424686_01_CJ-9-16-g005.jpg
F4
Identification of Clonal Immunoglobulin Heavy Chain Gene Rearrangements in non-denaturing polyacrylamide electrophoresis gels. Positions 1 and 9 polyclonal controls, positions 2 - 4, 10 - 12 samples, positions 5 - 8, 13 - 15 clonal controls. Requested sample at positions 3 and 11. M: PhiX174 / HaeIII digest ladder (New England Biolabs).
CJ-9-16-g006.jpg
PMC3424686_01_CJ-9-16-g006.jpg
F5
Identification of Clonal T Cell Receptor Gamma Chain Gene Rearrangements in non-denaturing polyacrylamide electrophoresis gel (PAGE). Positions 1 and 9 polyclonal controls, positions 2 - 5, 10 - 13 samples, and positions 6 - 8, 14, and 15 clonal controls. Requested sample at positions 5 and 13. M: PhiX174 / HaeIII digest ladder (New England Biolabs).
CJ-9-16-g007.jpg
PMC3424686_01_CJ-9-16-g007.jpg
F6