PMC3969642_01
(a) Hydrocele sac wall. (b) Hydrocele sac wall diffusely infiltrated by histiocytes having clear to multivacuolated cytoplasm (H and E, x100). (c) Large cells with moderate nuclear pleomorphism and clear/ vacuolated cytoplasm (H and E, x400)
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F1
Partially excised left hydrocele sac was received in multiple bits for histopathological examination [Figure 1a]. Histopathological examination showed fibrocollagenous wall of hydrocele sac that was diffusely infiltrated by large cells having clear to multivacuolated cytoplasm [Figure 1b]. Nuclei in few cells were pushed to the periphery with little distortion [Figure 1c].
(a) Mucicarmin-negative cytoplasm of the histiocytes (Mucicarmin, x400). (b-d) Negative epithelial membrane antigen, pan-Cytokeratin and calretinin (immunohostochemistry, x400)
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F2
Cytoplasm of the cells did not stain with Mucicarmin stain [Figure 2a]. In immunohistochemistry (IHC) staining, cells were negative with epithelial membrane antigen (EMA), pan-Cytokeratin and calretinin [Figure 2b-d].
Histiocytes show (a) strong diffuse cytoplasmic positivity with CD 68 (IHC, x100). (b) Nonspecific staining with carcinoembryonic antigen (IHC, x400). (c-e) Negative staining with cytokeratin 7, cytokeratin 20 and prostate-specific antigen (IHC, x400)
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F3
CD68 [Figure 3a]. Carcinoembryonic antigen (CEA) showed nonspecific staining [Figure 3b] and cytokeratin 7 (CK7), cytokeratin 20 (CK20) and prostate-specific antigen (PSA) were negative [Figure 3c-e].