PMC10202461_01
Location of the occurrence of autochthonous feline leishmaniasis
described in this study.
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In October 2019, a stray cat, approximately 3 years old, was taken to a veterinary service by a cat lover who had been feeding it for about 2 years at the door of his house, which is located in the COAB complex in Marambaia neighbourhood in Belem, the capital of Para state, Brazil (Figure 1).
Skin erythematous, edematous, and non-painful papular lesions in a
stray cat from an urban area in eastern Amazon.
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On physical examination, lymphadenopathy and erythematous, edematous and non-painful papular lesions around the edges of both ears, the snout, and the dewclaw of the left hind limb were observed in the cat (Figure 2).
Cytology of the aspirate (panoptic staining) from the nostril of the
animal indicating macrophages with amastigotes of
Leishmania sp. (Magnification A,
B and D: 1000x; C: 400x).
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However, the cytopathological examination confirmed amastigote forms of Leishmania sp. inside macrophages (Figure 3).
Phylogenetic tree derived from the Bayesian inference based on
partial SSU rDNA of Leishmania. Bayesian posterior
probability values are given above the branch nodes. The scale bar
indicates the number of substitutions per nucleotide site.
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According to the topology found (Figure 4) in group splits in three clades: A (sequence of this study, Leishmania infantum, Leishmania chagasi, Leishmania donovani), B (Leishmania amazonensis, Leishmania major) and C (Leishmania braziliensis, Leishmania guyanensis).