PMC10233592_01
Flow chart illustrating the manipulation of the collected testicular tissue
after TESE/mTESE.
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PMC10233592_01_10.1177_11795476231178353-fig1.jpg
fig1-11795476231178353
According to the scheme followed by the embryology laboratory in this fertility center (Figure 1), the samples were placed in Petri plates containing erythrocyte lysing buffer and HEPES-buffered Earle's medium supplemented with 0.5% human serum albumin (Sigma, Cairo, Egypt) and moved to the embryology laboratory. The embryologist plunged the processed tissue in liquid nitrogen except for a part, which was sent for the histopathological examination (Figure 1).