PMC4629570_01
Gross necropsy: lung tumours in an Asiatic lion with haemangiosarcoma.
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F1
Gross post-mortem examination revealed a normal body condition with a body weight of 140 kg and the following salient findings: anaemic mucosae; 10 litres of blood in the abdominal cavity; pale-coloured pancreas and spleen; multiple nodular lung masses (a few mm to 10 cm diameter, filled with blood clots) (Fig. 1); multiple (2 mm - 8 mm diameter) kidney cysts (filled with clear transparent liquid); multiple liver nodules (a few mm to 10 cm diameter, filled with blood clots, some with grapelike appearance, others were ruptured) (Fig. 2). Mitotic figures ranged from 1 to 4 per high power field.
Gross necropsy: liver tumours in an Asiatic lion with haemangiosarcoma.
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F2
Mitotic figures ranged from 1 to 4 per high power field.
Histopathology of liver haemangiosarcoma in an Asiatic lion. (A) The masses are composed of streams and capillary-like channels (asterisk) formed by polygonal to spindle shaped neoplastic cells. A mitotic figure is indicated (arrow). Mitoses range from 1 to 4 per high power field (Haematoxylin and eosin - H&E). (B) Cluster of differentiation 31 (CD31) positive immunohistochemistry. On the left side of the image CD31 negative hepatocytes are seen.
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F3
The masses were composed of loosely arranged streams and poorly defined capillary-like channels, supported by a moderate amount of eosinophilic stroma and occasionally large vascular channels, filled with variable amounts of blood (Fig. 3a). Mitotic figures ranged from 1 to 4 per high power field. The neoplastic cells labelled strongly positive for vWf and CD31 (Fig. 3b).