PMC1112608_01
Hyperintensity of the nodule on T1- and T2-weighted images (left), which remains so after gadolinium injection (right), on T1-weigthed image in the portal phase.
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F1
After gadolinium injection, the nodule was hyperintense on T1-weigthed images and remained so in the portal phase (Fig. 1).
Formalin fixed specimen: a flat and slightly clearer area is visible in the expected zone (arrow).
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F2
However, after formalin fixation, a 2 cm in diameter paler area was identified (Fig. 2, arrow).
Septal fibrosis in non tumoral liver (a), contrasting with absence of fibrosis in the nodule (b). Masson's trichrome.
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F3
The liver was fibrotic (METAVIR score F3) (Figs. 3a, 4a) with an iron overload 3 + (according to Searle score), mainly in hepatocytes of zones 1 and 2 (Fig. 5a). The architecture was preserved but the area was far less fibrotic (METAVIR score F1; Figs. 3b, 4b), with less iron (Fig. 5b), less glycogen (Fig. 6a), and with foci of clear hepatocytes (Figs. 6a, 6b).
Septal fibrosis in non tumoral liver (a), contrasting with absence of fibrosis in the nodule (b). Reticulin staining.
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F4
The liver was fibrotic (METAVIR score F3) (Figs. 3a, 4a) with an iron overload 3 + (according to Searle score), mainly in hepatocytes of zones 1 and 2 (Fig. 5a). The architecture was preserved but the area was far less fibrotic (METAVIR score F1; Figs. 3b, 4b), with less iron (Fig. 5b), less glycogen (Fig. 6a), and with foci of clear hepatocytes (Figs. 6a, 6b).
PAS staining: (a) foci of clear hepatocytes (arrow) close to the border between the non tumoral PAS positive zone, on the left side, and the PAS negative nodule on the right side of the photograph; (b) a clear focus in the nodule.
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F6
The architecture was preserved but the area was far less fibrotic (METAVIR score F1; Figs. 3b, 4b), with less iron (Fig. 5b), less glycogen (Fig. 6a), and with foci of clear hepatocytes (Figs. 6a, 6b).
Normal portal tract in the nodule. H&E staining.
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F7
One of the most striking findings was the presence of different types of portal tracts: some were normal (Fig. 7), whereas others contained mainly ductules (Fig. 8) and others arteries (Fig. 9).
An unpaired artery in the nodule. alpha-SMA immunostaining.
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F9
One of the most striking findings was the presence of different types of portal tracts: some were normal (Fig. 7), whereas others contained mainly ductules (Fig. 8) and others arteries (Fig. 9).