PMC8215559_01
Chromatogram from Sanger sequencing for the c.5612T>A variant using forward primer (top) and reverse primer (bottom).. Shown is the wildtype T allele with a small peak for the variant allele A (rectangular box).
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The peak height of the variant was approximately one-quarter to one-third that of the wild-type for both forward and reverse primers, confirming the mosaicism ( Figure 1).