PMC6430975_01
Normalization of STAT1 hyperphosphorylation in STAT1 GOF mutated patient after transplantation.. (A) Pre- and post-transplantation total p-STAT1 expression in CD4+ T cells stimulated with IFN-beta (20 ng/mL) by flow cytometry in patient (P1) compared to healthy control. (B) The dose response curve of STAT1 phosphorylation induced with IFN-beta in patient and control CD4+ T cells. *** p<0.0001 by two-way ANOVA. Tx: Transplantation.
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F1
STAT1 phosphorylation previously noted upon treatment of her CD4+ T cells with IFN-beta significantly decreased down to normal control levels following transplantation (p<0.0001, Fig. 1A and B).
Normalization of TH1 cell skewing and improved TH17 cell responses in STAT1 GOF mutated patient following transplantation.. Flow cytometric analyses of IL-17 and IFN-gamma expression in peripheral blood CD4+ and CD8+ T cells of P1 before and after transplantation as compared to a control subject.
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F2
Similarly, her dysregulated IFN-gamma/IL-17 CD4+/CD8+ T cells responses were also normalized (Fig. 2). She is now at 24 months after transplantation with continued 100% donor chimerism without medication.
PMC6430975_02