PMC10277675_01
Chromosomal microarray (CMA) and quantitative fluorescent (QF)-PCR results. (A) Chromosome X of CMA (red box) in uncultured CVS (upper) and AF (lower). (B) Markers on chromosome X of QF-PCR in uncultured CVS (upper), AF (middle), and maternal blood (lower).
fgene-14-1165019-g001.jpg
PMC10277675_01_fgene-14-1165019-g001.jpg
F1
Additionally, genomic DNA extracted from the peripheral blood of this woman was used to test for maternal cell contamination (MCC) (Figure 1). After counting 200 cell nuclei, we found an X/XX ratio of 4/196, practically ruling out a monosomy X cell line in uncultured CV (Figure 1).