PMC4086002_01
The tWE-lab system: (A) connection between tube 1 with citric acid and sodium bicarbonate in water and tube 2 with IVF culture medium transfers enough CO2 gas (B) produced by the chemical reaction between the acid and the base to equilibrate the pH of the medium to +/- 7.30. Fertilization and embryo scoring by looking through the glass tube (D,E).
FVVinObGyn-6-45-49-g001.jpg
PMC4086002_01_FVVinObGyn-6-45-49-g001.jpg
F1
IVF culture system (SCS) was compared with regular IVF culture system (RCS), described by Van Blerkom et al. (2014). In the first (fresh) IVF attempt 14 oocytes were retrieved, a single embryo transfer (SET) from SCS was performed according to the protocol of the study (Figure 1a).
PMC4086002_02
Description of three cases of live births after freezing/thawing of embryos obtained with the tWE lab simplified IVF procedure (SCS = simplified culture system or tWE lab system, RCS = regular culture system, SET = single embryo transfer, DET = double embryo transfer, PCOS = polycystic ovary syndrome, CC = clomiphene citrate, NC = natural cycle, EP = estrogen / progesterone scheme).
FVVinObGyn-6-45-49-g002.jpg
PMC4086002_03_FVVinObGyn-6-45-49-g002.jpg
F2
All embryos (n = 9) were cryopreserved (Figure 2c).
PMC4086002_03
Description of three cases of live births after freezing/thawing of embryos obtained with the tWE lab simplified IVF procedure (SCS = simplified culture system or tWE lab system, RCS = regular culture system, SET = single embryo transfer, DET = double embryo transfer, PCOS = polycystic ovary syndrome, CC = clomiphene citrate, NC = natural cycle, EP = estrogen / progesterone scheme).
FVVinObGyn-6-45-49-g002.jpg
PMC4086002_03_FVVinObGyn-6-45-49-g002.jpg
F2
All embryos (n = 9) were cryopreserved (Figure 2c).