PMC3557982_01
Hematoxylin and eosin-stained lung tissue (original magnification x10) from a patient in New Brunswick, Canada, with a fatal case of pneumonia caused by adenovirus 14. A) Arrow shows a patchy area of hemorrhagic consolidation. B) Arrow shows an area of necrosis.
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F1
Examination by microscopy showed diffuse alveolar damage, fibrinohemorrhagic changes, areas of necrosis, and cellular debris (Figure 1).
Phylogenetic analysis of human adenovirus 14 (HAdV-14) isolates from patients in New Brunswick, Canada, 2011. Nucleotide sequences were determined for the fiber (A), E1A (B), and hexon (C) genes. The corresponding gene sequences from previously reported HAdV-14 isolates are also included. Phylogenetic analysis was performed by using the neighbor-joining method of the MEGA2 program. Scale bars indicate nucleotide substitutions per site. Numbers on branches and at nodes indicate bootstrap proportions. The GenBank accession numbers for the fiber, E1A, and hexon genes for HAdV-14 (Canada) RV1360 are JQ815083, JQ815080, JQ815086, respectively; those for RV1368 are JQ815084, JQ815081, JQ815087, respectively; and those for RV1370 are JQ815085, JQ815082, JQ815088, respectively.
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F2
We performed nucleotide sequencing of the E1A, fiber, and hexon genes of the HAdV-14 isolates from Canada and compared the sequences with those for published adenovirus subspecies B2 (type 11, 14, 34, and 35) (Figure 2).