PMC6871352_01
Histologic examination of primary peritoneal high-grade serous carcinoma (HGSC).. Hematoxylin and eosin (H&E) staining, original magnification 1A:X4; 1B:X10.
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The final histology with hematoxylin and eosin staining revealed two synchronous tumors: SBT of the right ovary and HGSC of probably primary peritoneal origin, FIGO stage IIIC ( Figure 1A-B and Figure 3A-B). Morphologically, the peritoneal implants displayed branching papillary fronds, slit-like fenestrations, glandular complexity, moderate to marked nuclear atypia with marked pleomorphism, prominent nucleoli, stratification, frequent mitoses and stromal invasion (irregular or destructive infiltration by small glands or sheets of cells), strongly indicating HGSC ( Figure 1A-B). By IHC, the HGSC specimen showed positivity for PAX8, WT-1, aberrant p53 expression and high proliferation index, while calretinin and CD10 staining were negative ( Figure 1 and Figure 2).
Strong and diffuse immunoexpression of p53 in primary peritoneal high-grade serous carcinoma (HGSC), original magnification X10.
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By IHC, the HGSC specimen showed positivity for PAX8, WT-1, aberrant p53 expression and high proliferation index, while calretinin and CD10 staining were negative ( Figure 1 and Figure 2).
Histologic examination of serous borderline tumor (SBT).. Hematoxylin and eosin (H&E) staining, original magnification 3A: X4; 3B:X10.
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The final histology with hematoxylin and eosin staining revealed two synchronous tumors: SBT of the right ovary and HGSC of probably primary peritoneal origin, FIGO stage IIIC ( Figure 1A-B and Figure 3A-B). Conversely, features suggestive of SBT/APTS were found in the right ovary, namely broad, branching papillae (hierarchical branching) focally covered by stratified epithelium with mild to moderate atypia with few mitoses ( Figure 3A-B). In contrast, SBT specimen was characterized by expression of WT1 and PAX8 and both estrogen and progesterone receptors, and p53 was wild-type ( Figure 3 and Figure 4).
Wild-type immunoexpression of p53 in serous borderline tumor (SBT), original magnification X40.
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In contrast, SBT specimen was characterized by expression of WT1 and PAX8 and both estrogen and progesterone receptors, and p53 was wild-type ( Figure 3 and Figure 4).