PMC3825558_01
PMC3825558_02
General pathology of the aortic valve. Toluidine blue stain shows lysosomal accumulation of glycosaminoglycans
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Fig1
Toluidine blue staining of the aortic valve demonstrated cellular accumulation of GAGs (Fig. 1). 
Electron microscopy of the aortic valve. a
Arrow shows a vacuolated histiocyte, indicating lysosomal accumulation of glycosaminoglycans. b,
c In the lysosomes, zebra body formation indicating lysosomal accumulation of glycosaminoglycans can be seen
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PMC3825558_02_246_2013_653_Fig2_HTML.jpg
Fig2
In addition, we observed zebra body formation, which indicated glycolipid accumulation (Fig. 2). 