PMC3420675_01
Blasts appear of medium large size, with nucleoli, without cytoplasmic granules.
CRIM.HEMATOLOGY2011-230568.001.jpg
PMC3420675_01_CRIM.HEMATOLOGY2011-230568.001.jpg
fig1
The bone marrow aspirate was hypercellular with blasts of medium-large size, with nucleoli, without cytoplasmic granules, and with occasional "hand mirror" forms (Figure 1).
Blasts are shown in blue, lymphocytes in violet. Blasts are CD34+/-CD7+HLA-DR+CD38+CD99+CD3-cyCD3- CD19-CD20-.
CRIM.HEMATOLOGY2011-230568.002.jpg
PMC3420675_01_CRIM.HEMATOLOGY2011-230568.002.jpg
fig2
The blasts were positive for CD34+/-HLA-DR, CD7, CD99, CD38 (Figure 2), CD45RA, CD10+/-, and CD4 (not shown). Conventional cytogenetics performed on the bone marrow revealed a 47, XY karyotype with trisomy of chromosome 8 (47, XY, +8). T-cell receptor (TCR) delta gene rearrangements studies by polymerase chain reaction (PCR) showed the presence of a clonal TCR rearrangement and therefore a clonal T-cell population.