PMC4794595_01
Avascular whitish plaque-like lesion is observed in the cornea.
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fig1
A 76-year-old man consulted us for visual disturbance with corneal opacity in his left eye (Figure 1).
(a) One month after the removal of the lesion. The lesion did not recur. (b) Stroma beneath the original tumor is somewhat opaque (arrow).
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fig2
No recurrent lesion was observed after seven months of surgery (Figure 2(a)). Stroma beneath the neoplasm is opaque (Figure 2(b)).
Hematoxylin-eosin (HE) staining shows that the epithelium is occupied with dysplastic cells with differentiation tendency from basal layer toward superficial layer.
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fig3
The epithelium of the lesion was occupied with atypical dysplastic epithelial cells as revealed by HE staining (Figure 3).
Immunohistochemical detection of intermediate filament components in dysplastic epithelium. Keratin 12 (a) is not expressed in dysplastic epithelial cells (asterisk), while it is well detected in normal corneal epithelium (arrow) adjacent to the lesion. Keratin 13 (b) and keratin 14 (c) are readily detected in the dysplastic cells throughout the layers. Some of the dysplastic cells are labeled for vimentin (d).
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fig4
Immunohistochemistry of cell lineage-specific intermediate filaments, for example, vimentin of mesenchymal cells, keratin 14 of a basal cell of stratified epithelium, keratin 12 of corneal epithelium, and keratin 13 of conjunctival epithelium, showed that the dysplastic cells were positively labeled for vimentin, keratin 13, and keratin 14, but not for keratin 12 (Figure 4).