PMC9046972_01
Imaging results in the intracranial lesion tissue. The results of MRI showed the lateral ventricle expansion, with abnormal signal mass shadow (size about 3.3x3.1x3.5cm) in the lateral ventricle. (A, B: sagittal position; C, D: horizontal position). MRI, Magnetic resonance imaging.
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Then, the child went to the hospital for magnetic resonance imaging (MRI) examination, which showed an intracranial mass in the lateral ventricles ( Figure 1).
Histopathological results in the intracranial lesion tissue. (A) Hematoxylin-eosin staining in the intracranial lesion tissue showed abundant cytoplasm in the ventricular soft tissue mass cells with foamy or eosinophilic histiocytic infiltration (scale bar is 50mum). (B) Immunohistochemical CD1a staining showed negative tissue cells (scale bar is 50mum). (C) Immunohistochemical langerin staining showed negative tissue cells (scale bar is 50mum). (D) Immunohistochemical Ki-67 staining showed a small amount of positive tissue cells (scale bar is 50mum).
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In addition, the hematoxylin-eosin (H&E) staining in the intracranial lesion tissue showed abundant cytoplasm in the ventricular soft tissue mass cells with foamy or eosinophilic histiocytic infiltration, and the histiocytes stained negatively for CD1a and langerin, and also found a Ki67-positive rate of approximately 5% (Figure 2).
Histopathological results in the intracranial lesion tissue. (A) Immunohistochemical CD68 staining showed positive tissue cells (scale bar is 50mum). (B) Immunohistochemical S-100 staining showed weakly positive tissue cells (scale bar is 50mum). (C) Immunohistochemical CD163 staining showed positive tissue cells (scale bar is 50mum). (D) Immunohistochemical GFAP staining showed negative tissue cells (scale bar is 50mum). GFAP, Glial fibrillary acidic protein.
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The histiocytes stained positively for CD68, CD163, and negatively for glial fibrillary acidic protein (GFAP) and were partially positive for S-100 by immunohistochemical assay (Figure 3).
The results of the BRAFV600E
 mutations in the intracranial lesion tissue. (A) Immunohistochemical BRAFV600E
 staining showed positive tissue cells (scale bar is 200/50mum). (B) and the BRAFV600E
 mutations was detected by RT-qPCR.
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Therefore, to confirm this hypothesis, we further tested for the presence of BRAFV600E  mutation in diseased tissue, then the pathological results confirmed positive BRAFV600E  staining, and the BRAFV600E  mutations was also detected by real-time quantitative PCR (RT-qPCR) in the intracranial lesion tissue (Figure 4).