PMC7523626_01
Wound lesion on the right foot of the patient. (A) lesions on the toes, (B) extended lesion on her sole.
acmi-2-106-g001.jpg
PMC7523626_01_acmi-2-106-g001.jpg
F1
The patient returned again about a month later due to failure to respond to treatment, although she reported painful scarring and pale discharge; lesion depth was 1.5 cm and also extended to her sole (Fig. 1).
Colony morphology (a) and acid-fast staining (b) of 
Mycobacterium arupense
.
acmi-2-106-g002.jpg
PMC7523626_01_acmi-2-106-g002.jpg
F2
Two weeks later small colonies appeared on LJ enriched in Sauton's broth (Fig. 2).
16S rRNA sequence-based phylogenetic tree of our isolate and closely related mycobacterial species which are conducted using mega with the neighbour-joining method and K2P distance.
acmi-2-106-g003.jpg
PMC7523626_01_acmi-2-106-g003.jpg
F3
A phylognic-relationship analysis based on closely related mycobacterial species also identified the isolate as  M. arupense  as accession number: MN865166 (Fig. 3).