PMC3885775_01
PMC3885775_02
Interphase FISH results of case 2. (A) FISH analysis using a BCR/ABL1 Dual color, Dual fusion Translocation Probe, which showed a green signal (native BCR), a red signal (native ABL1), a fused signal, and a smaller green signal, indicating BCR-ABL1 and a loss of ABL1 in neutrophils as well as blasts: nuc ish(ABL1X2),(BCRX3),(ABL1 con BCRX1)[390/400]. (B) FISH analysis using BCR/ABL1 extra signal (ES) Dual Color Translocation Probe, which revealed a green signal (native BCR), a red signal (native ABL1), and a fused signal without a smaller red signal (ES) in neutrophils as well as blasts. The absence of the smaller red signal indicates the loss of ABL1. While using BCR/ABL1 ES probe, if there is no loss of ABL1, a fusion signal and a smaller red extra signal indicate major BCR-ABL1, and 2 fusion signals indicate minor BCR-ABL1. The results of case 2 indicate major BCR-ABL1: nuc ish(ABL1X2),(BCRX2),(ABL1 con BCRX1)[390/400].
alm-34-60-g001.jpg
PMC3885775_02_alm-34-60-g001.jpg
F1
BM study revealed 100% cellularity and 68% blasts. Both blasts and mature neutrophils showed the BCR-ABL1 fusion signals (Fig. 1).
PMC3885775_03
PMC3885775_04