PMC8866983_01
The results of basophil activation test.
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The basophil activation test was analysed by the Flow CAST kit (BUHLMANN, Schonenbuch, Switzerland), as shown in Figure 1.
Immunoblot and inhibition analysis. The serum-specific IgE bind to royal jelly and honeycomb. Line 1: royal jelly, serum; Line 2: royal jelly, serum co-incubated with honeycomb; Line 3: honeycomb, serum; Line 4: honeycomb, serum co-incubated with royal jelly.
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The proteins of bee venom, royal jelly and honeycomb were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting analysis of royal jelly extract (Figure 2) revealed serum IgE antibodies reacting only 60 kDa protein.