PMC3792539_01
Growth of P. damselae and V. harveyi on Columbia agar, (a) following 24 and (c) following approximately 48 hrs of incubation, as observed from the original inoculation. The colony marked with V. harveyi in (c) has a diameter of 2-3 mm. Note that (a), (b), and (d) were taken with flash against transmitting sunlight while (c) was taken with flashlight from above only. The section in (a) shows the growth after 24 hrs at 35 +- 1 C, and (b) is taken at the same time, but illustrating the hemolysis of P. damselae. (c) was taken following additional 24 hrs of incubation, however, at room temperature. While the images (a) and (c) show almost identical sections of the agar plates, they are not superimposable due to variations in the position. (d) illustrates the differences in color, seen especially after approximately 48 hrs, when V. harveyi was allowed to grow at a more ambient temperature (i.e., room temperature).
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fig1
With the second visual inspection, few additional larger colonies became apparent, which were not distinguished with the previous reading of the plates (Figure 1).
Wound, following seven days of ofloxacin (Tarivid) and following daily debridement, eight days after opening by removing the surgical threat. (Bar at the top is in cm.)
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PMC3792539_01_CRIM.MEDICINE2013-610632.002.jpg
fig2
The wound improved well under empiric ofloxacin therapy and daily care (debridement) (Figure 2).