PMC9932277_01
Timeline of events illustrating the red panda symptoms, treatment, and testing results.
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F1
On August 3, the red panda began to show abnormal behavior and was reported as lethargic, anorexic, with abdominal discomfort and vomiting (Figure 1).
Phylogenetic tree using kSNP analysis shows strain RedPanda1 grouping with Leptospira kirschneri serovar Grippotyphosa and separate from L. interrogans isolates. For those genome sequences downloaded from GenBank, accession numbers are provided followed by species_serogroup_serovar_strain. NVSL in-house genome sequences are indicated with an asterisk (*) followed by species_serogroup_serovar_strain.
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F2
The complete genome sequence was compared to other genomes of L. kirschneri including strains of L. kirschneri belonging to serogroups Grippotyphosa and Cynopteri, as well as genomes of L. interrogans with strains of L. interrogans belonging to serogroup Grippotyphosa and Icterohaemorrhagiae using kSNP analysis (Figure 2). The genome of strain RedPanda1 aligned most closely with L. kirschneri isolates, specifically strains belonging to serogroup Grippotyphosa serovar Grippotyphosa (Figure 2).