PMC7750336_01
(a) Family pedigree. (b) Gel appearance of whole gene deletion belonging to SLCO1B1. (c) Gel appearance of duplex PCR. (d) Sequence electropherogram of deletion breakpoints ([hg19] g. (21,017,795-21,402,024)x0)
TPA-55-430-g001.jpg
PMC7750336_03_TPA-55-430-g001.jpg
F1
Polymerase chain reaction (PCR)-based genotyping showed full deletion of SLCO1B1, also in case 1 (Fig. 1b). SLCO1B3 amplified. Subsequently, we confirmed our finding with gel electrophoresis pattern in our two patients, their parents, and in two individuals who were previously proven to be heterozygous (Fig. 1c). In the next step, we aimed to identify the part that was exposed to deletion using sequence analysis (Fig. 1d).
PMC7750336_02
PMC7750336_03
(a) Family pedigree. (b) Gel appearance of whole gene deletion belonging to SLCO1B1. (c) Gel appearance of duplex PCR. (d) Sequence electropherogram of deletion breakpoints ([hg19] g. (21,017,795-21,402,024)x0)
TPA-55-430-g001.jpg
PMC7750336_03_TPA-55-430-g001.jpg
F1
Polymerase chain reaction (PCR)-based genotyping showed full deletion of SLCO1B1, also in case 1 (Fig. 1b). SLCO1B3 amplified. Subsequently, we confirmed our finding with gel electrophoresis pattern in our two patients, their parents, and in two individuals who were previously proven to be heterozygous (Fig. 1c). In the next step, we aimed to identify the part that was exposed to deletion using sequence analysis (Fig. 1d).