PMC3563625_01
Abdominal CT scans in the patient. Abdominal CT scan was performed in the patient and showed two soft tissue masses in the pelvic cavity (one measuring 7.4x6.4 cm and the other measuring 8.0x4.6 cm). No abnormalities were seen in the bladder, liver, cervical stump or rectum. The arrows indicated the messes.
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F1
No abnormalities were seen in the bladder, liver, cervical stump or rectum (Figure 1).
Light microscopic examination of the tumor cells. The tumors removed were processed and slides were washed and counterstained with H&E stain (200x), observed under light microscopy showing polygonal and oval in shape. The tumors were composed of sheets, nests and trabeculae of cells with distinct borders and containing moderate to abundant amounts of eosinophilic cytoplasm. The nuclei were oval and located in the center of the cells. The arrows indicate the tumor cells.
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F2
Most tumor cells had distinct borders and were arranged in sheets, nests or trabeculae (Figure 2).
Immunohistochemical staining of AFP, p53, CK7 and alpha-inhibin in tumor cells. Tumor tissues were inflated, fixed in formalin, paraffin-embedded and sectioned for histological analysis. Slides were washed and counterstained with antibodies to AFP, p53, CK7 and alpha-inhibin using immunohistochemistry staining kits (Maxin BIO, Fu Zhou, China). The results showed that the hepatoid cells were focally positive for AFP (A), positive for p53 (B) and CK7 (C), and alpha-inhibin (D)) (Original magnification, 200x).
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F3
The results showed that hepatoid cells were positive for AFP, p53 and CK7 and focally positive for alpha-inhibin, but negative for CK20, CDX-2, Vimentin, CA-125, OCT3/4, NSE, hepatocyte paraffin 1, WT1, PLAP, AG/AG, polyclonal CEA, EMA, CD10 and CgA (Figure 3).