PMC6359751_01
CD20 B-cell stain showing a nodular pattern with widened marginal zones
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Fig1
Flow cytometry (on the FNA and the excision specimen) demonstrated a B-cell clone expressing CD19, CD20 (see Fig. 1), CD5, CD38, partial CD23, partial FMC7 and moderate kappa light chain.
GTG-banded karyotype from the lymph node of the patient showing a t(2;14) and gains of chromosomes 3, 7 and 18
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Fig2
There was an apparently balanced translocation between the short arm of one chromosome 2 at band p24 and the long arm of one chromosome 14 at band q32 (see Fig. 2).
Metaphase FISH analysis using the Vysis IGH break-apart probe. The normal chromosome 14 shows colocalization of the two probes, the derivative chromosome 14 has retained the 3'IGH signal (spectrum orange) and the 5'IGH signal (spectrum green) has translocated to chromosome 2
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Fig3
The IGH probe showed a break-apart signal with the 5' IGH signal on the derivative chromosome 2, the 3' IGH signal remained on the derivative chromosome 14 (see Fig. 3).
Metaphase FISH analysis using the RP11-542H15 probe (spectrum green) and the Vysis MYCN probe (spectrum orange). The normal chromosome 2 shows colocalization of the two probes, the derivative chromosome 2 has retained the RP11-542H15 probe (spectrum green) and the MYCN probe (spectrum orange) has translocated to chromosome 14
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Fig4