PMC5436409_01
Travel route of index case with Ebola virus disease from Liberia to Lagos, Nigeria. Lower inset shows the closest health care facility to the airport (LASUTH), the private health care facility where the index case was admitted (Private Hospital), the Laboratory where the diagnosis was made (Virology Lab, CMUL/LUTH) and the Genomics Centre where the corroborative diagnosis and nucleotide sequencing was done (ACEGID). Map was plotted with GPS coordinates using Arc GIS 10.1.
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Upon arrival at Lagos International Airport on 20 July 2014, he collapsed and was taken to a private hospital in the Obalende area of Lagos, Nigeria (Figure 1), where he was admitted.
Agarose gel image showing PCR amplicon bands of the specimens. The ~300 bp band is the target band size for Filoviruses.
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The FASTA format of the L-gene region of all the selected genomes was downloaded and aligned with the KM251803.1 sequence using the MUSCLE tool of MEGA-6 software (Figure 2). Investigations carried out on viral RNA purified from the blood and urine specimens of the patient revealed the presence of the expected amplicon size (300 bp) for Filoviruses (Figure 2).
Molecular phylogenetic analysis of the L-gene segment of EBOV/Hsap/NGA/2014/LIB-NIG 01072014 in comparison with selected filoviruses sequences by the maximum likelihood method. Sequences are labelled using the ICTV consensus nomenclature for variants of the Filoviridae family with their corresponding Genebank Accession numbers in parenthesis.
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Phylogenetic analysis of the L-gene partial CDS indicated that the virus evolved from the Guinea and Mano River isolates of the 2014 outbreak with a bootstraping value of 87% (Figure 3).