PMC9529529_01
Histopathologic examination of the excised cervical lymph node revealed multiple cystic epithelial structures lined by stratified squamous epithelium exhibiting to a great extent malignant cytologic (hyperchromatic, irregular nuclei, mitotic figures) and architectural (loss of polarity) features (a); lying on dense lymphatic tissue focally forming lymphoid follicles with prominent germinal centers (b). The squamous cell epithelium lining of the cysts exhibited areas of transition (b, c) of moderate/severe dysplasia to in situ squamous cell carcinoma (d). A well-defined sinus tract was not present. Hematoxylin and eosin-stained slides of the cervical lymph node (a-d) at X40 (a, b, and c); and x200 (d) magnification.
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fig1
Immunohistochemistry was positive for KYMB and p63, but negative for p16 (Figure 1).
Crypt of the left palatine tonsil lined by squamous cell epithelium lining showing areas of dysplasia and progression (a) to infiltrative moderately differentiated keratinizing carcinoma in the form of solid coalescent aggregations with foci of keratinization and comedo necrosis neoplastic squamous cells with frank atypia (b). Immunohistochemically, the neoplastic cells were positive for p63 (c) and showed strong and diffuse positivity for p16 (d). Hematoxylin and eosin-stained slides of the left palatine tonsil (a-d) at x20 (b, c, and d) and x40 (a) magnification.
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fig2
Iotammunohistochemistry was p16+, p53+ in 2-3%, and PD-L1+ localized in 2-3% (Figure 2).