PMC9425538_01
PMC9425538_02
Multiplex PCR assay for simultaneous detection of 16S rRNA (~900 bp), as an amplification control, and carbapenemase genes. BlaKPC: Lane 1, K. pneumonia IOC4955 (positive control); Lane 2, K. pneumonia ATCC 700603 (negative control); Lane 3, 5843; Lane 4, 5939; Lane 5 5958. BlaNDM: Lane 1, E. cloacae CCBH10892 (positive control); Lane 2, K. pneumonia ATCC 700603 (negative control); Lane 3, 5843; Lane 4, 5939; Lane 5 5958. BlaOXA-48: Lane 1, K. pneumoniae CCBH9976 (positive control); Lane 2, K. pneumonia ATCC 700603 (negative control); Lane 3, 5843; Lane 4, 5939; Lane 5 5958. BlaVIM: Lane 1, P. aeruginosa CCBH11808 (positive control); Lane 2, K. pneumonia ATCC 700603 (negative control); Lane 3, 5843; Lane 4, 5939; Lane 5 5958. BlaIMP: Lane 1, K. pneumonia BR01 (positive control); Lane 2, K. pneumonia ATCC 700603 (negative control); Lane 3, 5843; Lane 4, 5939; Lane 5 5958. Lane M, 100-bp DNA ladder (Invitrogen). The electrophoresis was run in a 2% agarose gel, which was stained with ethidium bromide.
gr1.jpg
PMC9425538_02_gr1.jpg
fig0005
Fig. 1 shows banding patterns of multiplex PCR reactions for the genes studied.