PMC3529828_01
Designs of leprosy primers (A) and differential polymerase chain reactions (B) for the 16S rRNA gene.
nihms402521f1b.jpg
PMC3529828_01_nihms402521f1b.jpg
F1
The designs of primers and PCRs are shown in Figure 1, and they aim at the 16S rRNA gene. Because these sequences spanned the M. lepromatosis-specific primer LPMF2 (Figure 1A), they also corroborated the PCR specificity.
Detection of Mycobacterium lepromatosis, but not Mycobacterium leprae, by differential polymerase chain reactions for the 16S rRNA gene. Lane 1: DNA size marker; lanes 2, 3, and 4: case Mn-1 with no amplification of the 447-bp, 417-bp, or 411-bp that target both and each species respectively; lanes 5 and 8: common amplicon of 171-bp for cases Mn-1 and S63 respectively; lanes 6 and 9: amplicon of 143-bp of M. lepromatosis for cases Mn-1 and S63 respectively; lanes 7 and 10: lack of the 135-bp amplicon of M. leprae in each case.
nihms402521f2.jpg
PMC3529828_02_nihms402521f2.jpg
F2
We detected the common mycobacterial target as well as the M. lepromatosis-specific amplicon, but not the M. leprae-specific amplicon (Figure 2, lanes 8-10).
Demonstration of the acid-fast bacillus Mycobacterium lepromatosis in the lymph node tissue in case S63 (Fite, x 1000).
nihms402521f3.jpg
PMC3529828_02_nihms402521f3.jpg
F3
These nodes showed florid foamy histiocytes filled with AFB (Figure 3). We detected the common mycobacterial target as well as the M. lepromatosis-specific amplicon, but not the M. leprae-specific amplicon (Figure 2, lanes 8-10).
PMC3529828_02
Detection of Mycobacterium lepromatosis, but not Mycobacterium leprae, by differential polymerase chain reactions for the 16S rRNA gene. Lane 1: DNA size marker; lanes 2, 3, and 4: case Mn-1 with no amplification of the 447-bp, 417-bp, or 411-bp that target both and each species respectively; lanes 5 and 8: common amplicon of 171-bp for cases Mn-1 and S63 respectively; lanes 6 and 9: amplicon of 143-bp of M. lepromatosis for cases Mn-1 and S63 respectively; lanes 7 and 10: lack of the 135-bp amplicon of M. leprae in each case.
nihms402521f2.jpg
PMC3529828_02_nihms402521f2.jpg
F2
We detected the common mycobacterial target as well as the M. lepromatosis-specific amplicon, but not the M. leprae-specific amplicon (Figure 2, lanes 8-10).
Demonstration of the acid-fast bacillus Mycobacterium lepromatosis in the lymph node tissue in case S63 (Fite, x 1000).
nihms402521f3.jpg
PMC3529828_02_nihms402521f3.jpg
F3
These nodes showed florid foamy histiocytes filled with AFB (Figure 3). We detected the common mycobacterial target as well as the M. lepromatosis-specific amplicon, but not the M. leprae-specific amplicon (Figure 2, lanes 8-10).