PMC10180806_01
Forward grouping was done and it shows that the group had no agglutination in the Anti-A and Anti-B and no agglutination in the Anti-H, grouping was compared to control. The Rh typing was positive
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F1
Blood grouping was done for this patient forward grouping was done and we found no agglutination in the Anti-A and Anti- B and also in Anti-H [Figure 1] so we thought it to be bombay blood group.
The patient showed agglutination with pooled cells of A and B no agglutination with pooled cell of O and compared with control
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F2
We performed the reverse grouping and we found agglutination with pooled A cells and pooled B cells, but no agglutination in the pooled O cells [Figure 2].
Hemagglutination inhibition test was performed and control tube labeled as C shows clear haemaglutination and clear supernatant while the test tube labeled as T shows no hemagglutination that is secretor
AJTS-17-136-g003.jpg
PMC10180806_01_AJTS-17-136-g003.jpg
F3
We found forward and reverse grouping were discordant, so we came to the conclusion that the patient had Bombay variant blood group, We decided to check the secretor status of the patient by detection of substance in the saliva and performed by wieners hemagglutination inhibition test and we found that the patient had secretion of H substance in the saliva [Figure 3].