PMC4546963_01
Histological findings in the liver (hematoxylin and eosin stain). The hepatocytes are diffusely swollen with rarefaction of cytoplasm and accentuation of the cell membranes. Numerous hepatocytes exhibit glycogenated nuclei. There are fatty droplets presenting without inflammation and fibrotic changes.
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fig1
No fibrosis or inflammation was detected (Figure 1).
Histological findings in the liver (Periodic Acid-Schiff (PAS) stain). Cross section without showing zonal distribution pattern, having the macrovesicular steatosis and hydropic degeneration. In some areas PAS histochemical analysis showed hepatocyte cytoplasm filled with glycogen and glycogenic core (PAS, original size x40).
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fig2
Strong intracytoplasmic PAS staining was observed in favor of glycogen in the histochemical investigations (Figure 2).
Histological findings in the liver (Periodic Acid-Schiff (PAS) stain) after diastase digestion. The hepatocyte cytoplasm is not stained with PAS.
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fig3
After diastase digestion, which selectively degrades glycogen, PAS staining was no longer positive, confirming that glycogen accumulation was responsible for the findings (Figure 3).