PMC7662079_01
(A) Chest CT in case 1 before treatment. The lungs showed bilateral nodules. (B) Total body 18F-FDG PET. No hypermetabolic lesions other than the lesions in the lungs.
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F1
Chest X-rays showed multiple bilateral lesions in the lungs that were confirmed by the computed tomography (CT) scan in which multiple pseudonodular features surrounded by a ground glass halo were identified in both lungs (Figure 1A). A PET scan was performed, revealing evidence of multiple active sites in the lungs (Figure 1B).
Chest CT in case 1 after chemo- and radiotherapy treatment. After 1 year of treatment, the patient showed a complete remission of the disease.
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F2
The most recent chest CT (performed in January 2020) showed a complete remission of angiosarcoma (Figure 2).
PMC7662079_02
Immunohistochemical analysis and quantification of ALDH-positive cells in two cases of angiosarcoma of the lung. Paraffin-embedded tissues were stained with an ALDH antibody to detect positive cells in these two different cases of angiosarcoma of the lung. (A,B) Case 1 and (C,D) Case 2. (E,F) Isotype control of anti-ALDH antibody. (G,H) Adenocarcinoma of the lung used as positive control of anti-ALDH antibody. Semi-quantitative method to measure ALDH based on the positivity of the tumor cells: 0 (<5% positive), 1 (5-25% positive), 2 (>25-50% positive), 3 (>50-75% positive), and 4 (>75% positive). Representative images are shown, with 10 x and 20 x magnification. Scale bar = 100 mum.
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F3
These two patient samples had a different expression of ALDH1A1 staining in the tumor fraction: for case 1, the score was 1 (<5-25% positive tumor cells), while in case 2 the score was 3 (>50-75% positive tumor cells) (Figure 3).