PMC3617891_01
A waxy, glossy, blackish-brown nodule with telangiectasia on the nose (a). A blue-whitish area with dotted macules is surrounded by arborizing vessels (b). Multiple palisaded basophilic tumor islands are surrounded by infiltrating lymphocytes. Original magnification x100 (c).
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F1
On his initial visit, physical examination revealed a waxy, blackish-brown nodule with telangiectasia on his nose (fig. 1a). The dermatoscopic findings revealed multiple blue-gray globules accompanied by arborizing vessels (fig. 1b). The histological findings revealed multiple, palisaded basophilic tumor masses surrounded by prominent infiltrating lymphocytes (fig. 1c), confirming our clinical diagnosis.
A paraffin-embedded tissue sample was deparaffinized and stained using anti-CD8 Ab (a), anti-CD163 Ab (b), Foxp3 Ab (c) or anti-MMP9 Ab (d). The sections were developed with liquid permanent red. Original magnification x200 (a-d).
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F2
M2 macrophages (fig. 2b) and Foxp3+ cells (fig. 2c). MMP9+ cells were detected in the CD163+ M2 macrophage-infiltrated areas (fig. 2d), and TIA-1+ cells were scattered around the tumor (data not shown).
Summary of the number of immunosuppressive cells versus cytotoxic cells in the tumoral areas. Five representative fields of each section were selected. The number of immunoreactive cells was counted using an ocular grid of 1 cm2 at a magnification of x400. Data are expressed as the means +- SD of the number of immunosuppressive cells versus cytotoxic cells in each tumoral area. * p < 0.05.
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F3
The number of immunoreactive cells was counted using an ocular grid of 1 cm2 at a magnification of x400 (fig. 3).