PMC3160007_01
Radiograph showing an osteolytic and osteoblastic intra-medullary tumor characteristic of osteosarcoma.
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Radiologic examination disclosed a bone lesion most consistent with osteosarcoma (Fig. 1).
A) Keratin pearl composed of well differentiated neoplastic squamous cells within a zone of chondroid (H&E, x40). B) Nest of malignant squamous cells showing intercellular bridges (H&E, x100).
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Sections of the biopsy and resection specimen revealed an osteocartilaginous neoplasm in which were scattered small islands of epithelioid cells focally showing keratin pearl formation (Fig. 2A). Intercellular bridges were visible (Fig. 2B).
Immunohistochemical stain for cytokeratins AE1,3 demonstrating nests of positively staining cells.
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Immunohistochemistry for cytokeratins AE1,3, CK 7, CK 5/6 and CAM 5.2 revealed the nests of epithelioid cells to stain strongly for cytokeratins (Fig. 3).