PMC9780583_01
Detection of CMV DNA in maternal and fetal samples. A: Nested PCR (168bp)- Amplified HCMV DNA of 1-mother blood, 2-placenta and of 3-fetus. Ethidium Bromide stained Agarose gel (2%) electrophoresis to detect CMV DNA in maternal blood (lane 1), placental tissue (lane 2), fetal DNA (lane 3), 100 bp ladder (lane 4), blank (lane 5), reaction Control (lane 6), negative control (lane 7), blank (lane 8), positive control (lane 9), positive control (lane 10), B: Primer sequence used for amplification of CMV DNA, C: Nucleotide sequences of CMV amplicons from mother, placenta and fetus tissue and its significant alignments with gene bank.
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fig1
Extracted RNA and DNA samples were subjected to SARS-CoV-2 PCR and nested PCR for CMV (Fig. 1 ) respectively.