PMC4739143_01
Plasma progesterone and estradiol levels in the aborting mare and normal pregnant mares. A) Progesterone
levels continuously increased after the middle of the 20th week of pregnancy and were abnormal from 32 weeks
of pregnancy (arrow). B) Estradiol levels decreased after 33 weeks of pregnancy but were within the standard
range. The standard ranges are shown by a dotted line and gray area. The dotted line represents the mean +-
2SD values (unpublished data), and the gray area is based on the cutoff value reported by Shikichi
et al.
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At 32 weeks of pregnancy, abnormal progesterone (5.18 ng/ml, standard range: 1.46-4.17 ng/ml) and CTUP (10.7 mm, standard range: 2.76-7.16 mm) values were observed without any clinical signs (Figs. 1 and 2).
Development of the combined thickness of the uterus and placenta (CTUP) in the aborting and normal pregnant
mares. CTUP was thicker from 32 weeks of pregnancy (arrow). The standard range was calculated as the mean +-
2SD.
jes-26-129-g002.jpg
PMC4739143_01_jes-26-129-g002.jpg
fig_002
At 32 weeks of pregnancy, abnormal progesterone (5.18 ng/ml, standard range: 1.46-4.17 ng/ml) and CTUP (10.7 mm, standard range: 2.76-7.16 mm) values were observed without any clinical signs (Figs. 1 and 2).
Changes of fetal heart rate (FHR) in the aborting and normal pregnant mares. FHR was decreased from 37
weeks of pregnancy and decreased to below the standard range (arrow), calculated as mean +- 2SD, from 38
weeks.
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FHR decreased from 38 weeks (Fig. 3), and the udder developed further at 40 weeks.
Gross (A), Grocott staining (B) and immunohistochemical staining (C) images of the placenta. The chorionic
aspect around the cervical star was white in color approximately 15 cm toward the cranium, and the wall was
thick. Mycelia positive for Anti-Aspergillus sp. antibody and Grocott staining were
observed in the chorionic tissue.
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Histopathologically, mycelia positive for anti-Aspergillus sp. antibody (AbD Serotec) and Grocott staining were observed in the placenta (Fig. 4).