PMC3643366_01
(a) Clusters of cells with round to oval nuclei, prominent large nucleoli, abundant pale finely vacuolated to granular cytoplasm, with fraying of the cytoplasmic margins (H and E, x200). (b) PAS stain with diastase digestion showing PAS-positive, diastase-resistant intracytoplasmic magenta pink granules and occasional crystals (arrow head) (PAS with diastase, x400). (c) Histology showing characteristic alveolar pattern separated by fibrovascular septae and comprising similar cells (H and E, x100). (d) PAS stain highlighting the diastase-resistant intracytoplasmic needle-like structures (arrow) (PAS with diastase, x400)
JCytol-30-58-g001.jpg
PMC3643366_02_JCytol-30-58-g001.jpg
F1
PAS stain showed diastase-resistant intracytoplasmic needle-like structures [Figure 1d].
Ultrastructure photomicrograph showing (a, b) Abnormal clustering of mitochondria (x4000 and x6000, respectively); (c) Prominent endoplasmic reticulum (x6000); (d) Few electron-dense deposits within vacuolar spaces (x5000)
JCytol-30-58-g002.jpg
PMC3643366_01_JCytol-30-58-g002.jpg
F2
Electron microscopy showed increased number and abnormal clustering of mitochondria, prominent endoplasmic reticulum, glycogen vacuoles, along with few electron-dense bodies [Figure 2].
PMC3643366_02
(a) Clusters of cells with round to oval nuclei, prominent large nucleoli, abundant pale finely vacuolated to granular cytoplasm, with fraying of the cytoplasmic margins (H and E, x200). (b) PAS stain with diastase digestion showing PAS-positive, diastase-resistant intracytoplasmic magenta pink granules and occasional crystals (arrow head) (PAS with diastase, x400). (c) Histology showing characteristic alveolar pattern separated by fibrovascular septae and comprising similar cells (H and E, x100). (d) PAS stain highlighting the diastase-resistant intracytoplasmic needle-like structures (arrow) (PAS with diastase, x400)
JCytol-30-58-g001.jpg
PMC3643366_02_JCytol-30-58-g001.jpg
F1
PAS stain showed diastase-resistant intracytoplasmic needle-like structures [Figure 1d].