PMC4531591_01
Atypical, large cells are scattered among small to medium sized lymphocytes on higher power.
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There were a few foci of nodular aggregates around the white pulp (Figure 1), which was composed mostly of atypical CD3 positive T lymphocytes, some of which were positive for EBV latent membrane protein (LMP) (Figure 2), P53, and KI-67 positive.
Infiltrating atypical large cells in spleen are positive for EBV-LMP-1.
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There were a few foci of nodular aggregates around the white pulp (Figure 1), which was composed mostly of atypical CD3 positive T lymphocytes, some of which were positive for EBV latent membrane protein (LMP) (Figure 2), P53, and KI-67 positive.
EBV PCR with EBNA 1 primer showing strong positive band at 138bp.. Lane M: size marker (100bp ladder, MBI). Lane 1: patient sampleLane 2: positive control. Lane 3: negative control
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EBV polymerase chain reaction (EBV-PCR) with EBNA 1 primer showed a strong positive band at 138bp (Figure 3).
TCR gene rearrangement using V1-8A, B/J1, J2, V10/J1, and J2 showing monoclonal bands by 2% gel electrophoresis.TCR gene rearrangement using V9, B/J1, J2, V11/J1, and J2 showing no monoclonal band.. Lane M: size marker (100bp ladder, MBI). Lane 1, 4, 6, 8: patient sample. Lane 2: positive control (V1-8A, B/J1, J2). Lane 3, 5, 7, 9: negative control
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TCRgamma gene rearrangement, PCR amplification using primers Vgamma 1-8A, B/Jgamma, Jgamma 2, Vgamma 10/Jgamma 1, and Jgamma 2 showed monoclonal bands by 2% gel electrophoresis (Figure 4). These findings indicated the monoclonal nature of the lesion.