PMC8674942_01
(A) Abdominal X-ray showing intramural bowel gas also known as pneumatosis intestinalis, (B) culture growth of cream-colored colonies on COS after 24 h of incubation at 37 C in the anaerobic chamber, (C)
Clostridium neonatale rod-shaped cells using Hitachi SU5000 scanning electron microscope. Scale bar and acquisition settings are shown on the original micrograph, and (D) sporulating C. neonatale cell.
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F1
Abdominal X-ray showed pneumatosis intestinalis (Figure 1A). Bruz, France). Colonies were cream-colored and opaque after 24 h of growth (Figure 1B). SU5000 scanning electron microscope (Hitachi High-Tech Corporation, Tokyo, Japan), colonies were bacilli (Figure 1C). Bacterial cells were Gram-positive (not shown). Sporulation was observed after 24 h under aerobic conditions (Figure 1D).
Whole-genome (nucleotides) based phylogenetic tree highlighting the position of C. neonatale strain Marseille-Q4564 relative to other closely related bacterial taxa.
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F2
Taken together, these results confirm the status of this strain belonging to the C. neonatale species (Figure 2).