PMC6374879_01
Modified CHUHE assay testing the patient's serum and erythrocytes. Addition of exogenous ceftriaxone increased hemolysis over baseline (p=0.02). Addition of PIC1 (0.75 mM) to the patient's serum, to which ceftriaxone was added, resulted in decreased hemolysis compared with the addition of ceftriaxone alone (p=0.03). Means +- SD are shown for 2 independent experiments.
CRIHEM2019-4105653.001.jpg
PMC6374879_01_CRIHEM2019-4105653.001.jpg
fig1
These experiments showed that adding ceftriaxone (10 mug/ml) to her serum increased complement-mediated hemolysis (p=0.02) of her erythrocytes (Figure 1).