PMC3636966_01
Representative HE and IHC results for the SRC gastric carcinoma. Low- (a) and high-power (b) views of the SRC gastric carcinoma. HE. Arrows indicate SRCs. High-power views of Cam5.2 (c) and CEA (d). IHC.
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F1
Histology Lab with a standard hematoxylin and eosin (HE) technique, employing progressive staining with the background cleaned with Define MX solution. The sections were then treated with a basic solution (Blue Buffer 8) that 'blues' the nuclei followed by counterstaining with eosin. Histological examination of the slides revealed gastric mucosa with numerous infiltrating small individual cells, typical of an SRC (also known as 'diffuse type') gastric carcinoma (fig. 1a, b, x100 and x400 magnification, respectively). As previously found, the SRCs showed weak to moderate staining for Cam5.2 and CEA (fig. 1c, d, respectively).
IHC result for Nampt staining in SRC gastric carcinoma. a Low-power view of the SRC gastric carcinoma stained for Nampt. The arrows indicate SRCs. b Close-up view of the SCR indicated by the white arrow in a.
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F2
Interestingly, Nampt, which is over expressed in gastric cancer compared to benign gastric epithelium, was only weakly expressed in the SRCs and at a level far lower than the benign surrounding gastric epithelial cells (fig. 2).