PMC4531650_01
Platelet concentrations measured at different times in blood samples with different anticoagulants. (KM; Kanamycin, Pre-supple.; Pre-supplementation of Kanamycin to EDTA before blood withdrawal, Post-supple.; Post-supplementation of kanamycin to EDTA blood after 30 min of sampling, RT; room temperature
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Then, we measured the platelet count and examined the blood cell morphology in all the above-mentioned samples immediately after blood withdrawal and after 30 min, 60 min and 120 min of incubation at room temperature or at 37 C (Figure 1).
The blood cell morphology of blood samples. A: Platelet clumps in EDTA-anticoagulated blood without supplementation of kanamycin. B: No platelet clumps in the samples supplemented with kanamycin to EDTA before blood withdrawal.
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The collection and examination of a sample anticoagulated with EDTA at 37 C and the addition of kanamycin to anticoagulant after blood withdrawal only partially prevented the clumping anticoagulant before blood withdrawal resulted in a complete prevention of platelet aggregation (Figure 2A, 2B).