PMC5322459_01
Histological examination of the lymph node. (a) Vaguely nodular lymphoid proliferation (arrow) in partially effaced architecture (HE x100). (b) Plasmablastic cells with large eccentric nuclei, with atypical and often multilobulated contours. The cytoplasm is acidophilic and relatively abundant (HE x400).
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fig1
Nodules were centered by aggregates of plasmablastic cells with atypical eccentric nuclei and often multilobulated contours (Figure 1(b)).
The "onion-skinning features" in some follicles of the adjacent parenchyma mimicked the Castleman disease (HE x200).
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fig2
HHV8/EBV-associated germinotropic lymphoproliferative disorder: large lymphoid cells showed negative immunostaining for CD20 (magnification x25) (a) and CD30 (magnification x100) (b). Intense immunostaining for CD38 (magnification x200) (c) and HHV8 (magnification x100) (d); EBER in situ hybridization positivity of many tumor cells (magnification x200) (e).
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fig3
They were negative for the other tested antibodies (AE1/AE3, CD20, CD3, cyclin D1, CD56, CD15, CD30, CD10, and bcl2) (Figures 3(a)-3(d)). In situ hybridization confirmed the monotypic kappa light chains and the EBV infection (EBER+) (Figure 3(e)).