PMC9334666_01
After hematoxylin-eosin (HE) staining, it is found that there is a fascicular proliferative smooth muscle-like spindle cell area outside the pelvic lymph nodes, which has a clear boundary with the surrounding tissues (A). Smooth muscle-like spindle cell nuclei have no atypia and are mostly arranged around reticular or sinusoidal cavities lined with flat endothelium. Residual lymph nodes can be seen in local areas between proliferative spindle cells (B). The scale represents 300 mum (A) and 100 mum (B).
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F1
See in (Figures 1A,B) for the H&E staining results of the lymph node LAM pathological sections.
Immunohistochemistry of LAM cells showed that the smooth muscle-like specific protein markers a-SMA (A) and desmin (B) were positive, the melanocyte marker HMB45 was focally positive (C), D2-40 was positive (D), ER and PR were negative (E,F), and beta-catenin was positive (G). The scale represents 300 mum (A-G).
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F2
Immunohistochemistry showed a-SMA positivity, Desmin positivity, HMB45 (scattered cells +), D2-40 (vessels +), beta-catenin (cytoplasmic +), ER, and PR were negative (see Figures 2A-G).