PMC2779292_01
Results of seminested polymerase chain reaction (PCR) for gltA gene and pap 31 gene of B. henselae. Lanes 2-4 for PCR of gltA gene (139 bp), lane 6-8 for PCR of pap31 gene (211 bp). Lane 1 and 5, DNA ladder marker (Bioneer, Daejeon, Korea); lane 2 and 6 positive control (Houston-1, ATCC 49882); lane 3 and 7, negative control; lane 4 and 8, lymph node tissue from the patient with cat scratch disease.
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F1
B. henselae DNA was detected from patient's lymph node aspirate (Fig. 1).
Partial pap31 sequences of two main genogroups B. henselae and case (red color-primer, blue color-different sequence between genogroups).
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F2
The patient's PCR product for gltA had a consistent sequence of B. henselae and for pap31 gene showed a consistent sequence corresponding to main genogroup of B. henselae Houston (Fig. 2).