PMC5424191_01
(a) Peripheral blood promyelocytes; (b) peripheral blood pseudo-Pelger-Huet neutrophils; (c) bone marrow effacement by promyelocytes at diagnosis.
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fig1
Peripheral blood promyelocytes, which accounted for 40% of nucleated cells, had regular nuclei, were hypergranular, and lacked Auer rods (Figure 1(a)) with increased numbers of pseudo-Pelger-Huet neutrophils noted (Figure 1(b)). The bone marrow was effaced by promyelocytes (Figure 1(c)) which stained strongly positive for Sudan Black.
Sanger sequencing demonstrating fusion of ZBTB16 exon 3 to RARA exon 2.
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fig2
A standardised reverse transcription-PCR approach did not detect PML-RARA transcripts, but, using primers previously described, a ZBTB16-RARA fusion transcript, consistent with fusion of ZBTB16 exon 3 fused to RARA exon 2, was detected and confirmed by Sanger sequencing (Figure 2).