PMC8763705_01
(A) Timeline of vaccination, diagnostic testing and whole genome sequencing. RT-qPCR results for the spike gene mutations (B) L452R, (C) E484K, and (D) N501Y. Orange represents the curves matching Beta VOC (L452, 484K, and 501Y) and purple represents the curves matching Delta VOC (452R, E484, and N501) defining SNPs. (E) Proportion of sequencing reads matching Beta or Delta VOC for the lineage defining mutations. Orange bars represent Beta VOC and purple bars represent Delta VOC. White bars represent the proportion of reads matching neither Beta nor Delta VOC.
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The timeline of this co-infection case is shown in Figure 2A. The mutation-specific RT-qPCR results for the spike gene mutations are shown for L452R (Figure 2B), E484K (Figure 2C), and N501Y (Figure 2D). As shown in Figure 2E, the number of reads containing the mutations associated with Delta VOC was lower compared to the reads with sequences associated with Beta VOC. The higher Beta to Delta VOC ratio of RNA sample input was also observed in the RT-qPCR data (Figures 2B-D).