PMC8285198_01
PNH flow cytometry. Lymphocytes (red), monocytes (green), and granulocytes (pink) were displayed on the CD 45 side scattered (SS) plot (a). The SS plot demonstrating fluorescein-labeled-proaerolysin- (FLAER-) negative different cell populations (b). 51% of monocytes and 52% of granulocytes had PNH immunophenotype; FLAER-/CD14-/CD55 and FLAER-/CD24-/CD55, respectively ((c)-(f)).
CRIHEM2021-6619177.001.jpg
PMC8285198_01_CRIHEM2021-6619177.001.jpg
fig1
Flow cytometry was positive for PNH (Figure 1).
Peripheral blood and bone marrow biopsy. The peripheral blood smear showed leukopenia with absolute neutropenia and thrombocytopenia. The observed neutrophils and lymphocytes were mature without significant morphological abnormality. Schistocytes were not present (a). Bone marrow biopsy showed large areas of the marrow that are less than 10% cellular (b); however, focally, the marrow is 40-50% cellular with erythroid predominant trilineage hematopoiesis (c). Immunohistochemical stains performed with appropriate controls demonstrated numerous E-cadherin-positive erythroid precursors (d).
CRIHEM2021-6619177.002.jpg
PMC8285198_01_CRIHEM2021-6619177.002.jpg
fig2
Schistocytes were not present (Figure 2(a)). Bone marrow biopsy showed large areas of the marrow that were less than 10% cellular (Figure 2(b)); however, focally, the marrow is 40-50% cellular with erythroid predominant trilineage hematopoiesis (Figure 2(c)). Immunohistochemical stains performed with appropriate controls demonstrated numerous E-cadherin-positive erythroid precursors (Figure 2(d)).