PMC7908326_01
phylogenetic NS5B radial tree comparison of patient (in red) assigned as a novel genotype 7b variant
PAMJ-36-232-g001.jpg
PMC7908326_01_PAMJ-36-232-g001.jpg
F1
Sanger sequencing to evaluate for RAS in the NS3, NS5A and NS5B HCV genes using pan genotypic primers and the web-based geno2pheno program (Table 1). Sequences were aligned with GenBank reference sequences using BioEdit and a NS5B neighbour-joining phylogenetic tree was constructed in Mega 6.0 with 1000 bootstrap resamplings (Figure 1).