PMC10213281_01
The cut surface of the liver is gray-yellow-grey-green nodules and fibrous scars at the center.
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F1
Gray-yellow, gray-green nodules were found on the cut surface, with a size of 2.8 cm x 2.7 cm x 2.5 cm, and fibrous scars at the center (Figure 1).
Hematoxylin and eosin staining was observed under microscope. (A) Low magnification view Clear boundaries and a thin capsule was observed surrounding the tumor, original magnification x6. (B) Intratumoral fibrous tissue was arranged in parallel, separating tumor cell nests, original magnification x200. (C) The neoplastic hepatocytes have abundant deep eosinophilic cytoplasm, large nuclei, prominent nucleoli, and mitotic figures, original magnification x400. (D) There was a large amount of small lymphocytic cells of uniform shape infiltration between the nests of tumor cells, original magnification x50. (E,F) Some lymphoid cells were small, with few cytoplasm, round nuclei, basophilic nuclei, fine nuclear chromatin, unclear nucleoli, no cell atypia, and no lymphoepithelial lesions, original magnification x200, original magnification x400.
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F2
The cells were small to medium-sized, with few cytoplasm, round nuclei, basophilic nuclei, fine nuclear chromatin, indistinct nucleoli, lacked overt atypia, and no lymphoepithelial lesions observed (Figure 2).
Immunohistochemical expression of Fibrolamellar hepatocellular carcinoma. (A) HepPer-1. (B) Arginase-1. (C) CD34 microvascularization. (D) Focal and scattered CK7, original magnification x100.
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F3
Immunohistochemical profile of fibrolamellar hepatocellular carcinoma expresses HepPar1, Glypican-3, Arginase-1, focal or scattered positive for CK7(Figure 3), CD34 is positive in arterialized sinusoids, and there is low ki67 proliferation index, whereas CK19, RB, AFP, beta-catenin are not expressed.
Immunohistochemical expression of small lymphocytic cells of uniform, Lymphoid cells express T cell markers, including (A) CD3, (B) CD5, (C) CD43, (D) TDT, (E) highly express Ki-67 and (F) CD20 negative, original magnification x200.
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F4
The proliferative T-lymphoblastic cells to express T cell markers, including CD3, CD5, CD43, as well as TDT, and highly express Ki-67 (Figure 4), but do not express CD34, CD20, MPO, CyclinD1, CD10 and E-cad.