PMC9380824_01
Head and lung CT scans of the patient after admission in our hospital. (A). Head CT showing a small amount of blood, effusion, and swelling of the surrounding soft tissue in the basal ganglia after intracerebral hemorrhage surgery. (B). Lung CT showing no obvious signs of infection.
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Head CT after admission showed a small amount of blood and fluid accumulation in the operative area after intracerebral hemorrhage (Figure 1A), while lung CT showed no obvious signs of infection (Figure 1B).
mNGS analysis reveals the mapping and distribution of M. hominis reads. (A) Mapping of M. hominis reads on the genome with a coverage of 3.7%. (B) (left) Total read distribution in the CSF sample, and (right) read distribution without the human host.
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A total of 1424 reads were identified to correspond to the M. hominis genome, with a coverage of 3.7% (Figure 2A and B).
A 399-bp target fragment mapping to M. hominis, identified by sequence-specific PCR. Lane M: DNA ladder; Lane 1: negative control; Lane 2: annealing temperature of 58  C; Lanes 3 and 4: annealing temperature of 60  C.
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The results suggested a 399-bp target fragment aligned with the reference sequences of M. hominis (GenBank: CP055144.1) with 99.73% identity, thereby indicating M. hominis infection in this patient (Figure 3).