PMC3591034_01
(a) Intraoral photograph showing cystic cavity after surgical exposure. (b) Orthopantomograph showing well demarcated radiolucent lesion extending from mandibular first premolar to third molar.
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F1
Right submandibular lymphnodes were enlarged [Figure 1a]. Orthopantomograph revealed a well defined radiolucency involving right mandibular body extending from mandibular first premolar to third molar region distally [Figure 1b].
(a) Photomicrograph showing cystic cavity of odontogenic keratocyst (H and E, x100). (b) Photomicrograph showing eosinophilic vegetable matter in cyst wall (H and E, x100). (c) Photomicrograph showing foreign body giant cells (H and E, x40)
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F2
The epithelium demonstrated separation from underlying connective tissue in few areas [Figure 2a]. It demonstrated a double layered refractile hyaline membrane surrounded with prominent chronic inflammatory cells and few foreign body giant cells [Figure 2b].
(a) Photomicrograph showing vegetable matter demonstrating PAS positivity (PAS, 100x), (b) Photomicrograph showing vegetable matter demonstrating alcian blue positivity (Alcian blue, x100), (c) Photomicrograph showing vegetable matter demonstrating van Gieson negativity (van Gieson, 100x). (d) Photomicrograph showing vegetable matter demonstrating vegetable matter demonstrating birefringence under polarized microscope. (100x)
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F3
Periodic acid Schiff (PAS) without diastase, van Gieson and Alcian blue at pH 2.5 were performed. PAS [Figure 3a] and alcian blue [Figure 3b] demonstrated positivity for hyaline material confirming it to be vegetable matter. The surrounding condensed collagen stained reddish in color with van Gieson suggestive of matured collagen [Figure 3c].