PMC9978143_01
Conditioning regiment, immunosuppression and cellular reconstitution are shown for the first 100 days post HLA-haploidentical HSCT.
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TCR alpha/beta+/CD19+ depletion ( Figure 1 ). The cell product contained total nucleated cells 3.35 x 108 cells/kg, with CD34+, 53.96 x 106 cells/kg; CD3+ 53.28 x 106 cells/kg; TCR alpha/beta+ 12.31 x 103 cells/kg; and TCR gamma/delta+ 52.27 x 106 cells/kg. The patient received immunosuppressive therapy with mocophenolate mofetil (MMF) at the dosage of 2 x 600 mg/m2/d as GVHD prophylaxis from day + 1 until day + 28 ( Figure 1 ). Adenovirus was isolated day - 9 in the stool and urine samples and was treated with cidofovir until day + 50 ( Figure 1 ).
Donor Chimerism in peripheral blood for CD34+, CD3+, total cells (total chimerism) and respiratory burst in patient's neutrophils measured by DHR test are shown for the entire follow up time of 3 years.
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In parallel counts of CD3+ T-cell in peripheral blood also declined, whereas the respiratory burst of patient's granulocytes, measured by Dihydrorhodamine (DHR) assays upon stimulation with E. coli PMA continuously exhibited a normal production of H2O2 ( Figures 2 ,  3 ). In total, eight DLI were administered for rescue of the decreasing donor chimerism starting with a cell number of 2.5 x 104 CD3+ cells/kg and increasing up to 30 x 104 CD3+ cells/kg of patient's body weight between days + 66 and + 329 ( Figures 2 ,  3 ;  Table 1 ).
Immune reconstitution: The absolute cell numbers per mul are shown for the entire follow up time of 3 years. The CD45RA+ (CCR7+) percentage of the CD4+ cells are shown.
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In parallel counts of CD3+ T-cell in peripheral blood also declined, whereas the respiratory burst of patient's granulocytes, measured by Dihydrorhodamine (DHR) assays upon stimulation with E. coli PMA continuously exhibited a normal production of H2O2 ( Figures 2 ,  3 ). In total, eight DLI were administered for rescue of the decreasing donor chimerism starting with a cell number of 2.5 x 104 CD3+ cells/kg and increasing up to 30 x 104 CD3+ cells/kg of patient's body weight between days + 66 and + 329 ( Figures 2 ,  3 ;  Table 1 ).