PMC6077517_01
Two embryos after first cell division. The transferred embryo at 25.9 hours after insemination (a) and the embryo which was not transferred (b). Arrow shows fragmentations.
CRIOG2018-2152918.001.jpg
PMC6077517_01_CRIOG2018-2152918.001.jpg
fig1
On day 2 all embryos were with more than 60% fragmentation except 2 which were estimated with about 40% fragmentation (Figure 1). One of these was divided into 2 cells at about 25 hours after insemination (Figure 1(a)) and the other around 35 hours after insemination (Figure 1(b)). Following a discussion with the patient, we continued monitoring the embryos until day 5, at which time 1 blastocyst was developed (Figure 1(a)).
The transferred embryo from morula (81.6 hours) (a) to the blastocyst stage (115.2 hours), before (b) and after (c) collapse.
CRIOG2018-2152918.002.jpg
PMC6077517_01_CRIOG2018-2152918.002.jpg
fig2
This embryo had the expected annotation (according to the producer model) with first division at 24.7 h and start blastulation at 114 h (Figure 2).