PMC5477902_01
Admixtures of the affected patient serum with other bicarbonate sources yield predicted bicarbonate levels. Aliquots of affected serum (7.7 mEq/L) were mixed with either a bicarbonate solution (31.9 mEq/L) or a normal serum (25.6 mEq/L) at the indicated percentages. Measured bicarbonate levels in the admixtures were plotted together with the predicted bicarbonate values in the admixtures calculated from the sum of the individual component contributions.
sfm032fig1.jpg
PMC5477902_01_sfm032fig1.jpg
F1
Recovery of bicarbonate was at least 85-90% at all dilutions (Figure 1). As shown in Figure 2, a positive bias of 1.7 mEq/L was observed.
Bland-Altman plot of the difference between calculated bicarbonate and enzymatic measurement of serum bicarbonate. Data represent a series of 88 paired patient data sets of calculated bicarbonate, derived from the Henderson-Hasselbalch equation applied to arterial blood gas parameters and a matched serum bicarbonate measurement. Units are mEq/L. Solid line represents the mean difference or bias of 1.7 mEq/L. Several data points are superimposed.
sfm032fig2.jpg
PMC5477902_01_sfm032fig2.jpg
F2
As shown in Figure 2, a positive bias of 1.7 mEq/L was observed.