PMC3022785_01
Microscopic features of ovarian cyst in case 1. The cyst is lined by several layers of luteinized cells showing focal marked nuclear pleomorphism (A) and occasional mitotic figures (B). Reticulin fibers surround nests of lesional cells (C). Hematoxylin and eosin stain in A and B, and reticulin stain in C. Magnifications: x400 in A, B, and C.
1746-1596-6-3-1.jpg
PMC3022785_01_1746-1596-6-3-1.jpg
F1
On subsequent microscopic examination, the cyst was lined by single to multiple layers of large cells with abundant eosinophilic cytoplasm (Figure 1A). Most cells showed small, round and regular nuclei, but focal cells displayed enlarged and bizarre nuclei with hyperchromasia and occasional mitosis (Figure 1B). Special stain showed reticulin fibers around nests of luteinized cells in the cyst lining (Figure 1C) and in the outer cyst wall.
PMC3022785_02
Histologic characteristics of ovarian cyst in case 2. The cyst is lined by multiple layers of luteinized cells showing focal nuclear pleomorphism (A). The outer cyst wall shows nests of luteinized cells (B). Nests of lesional cells are surrounded by reticulin fibers (C). Hematoxylin and eosin stain in A and B, and reticulin stain in C. Magnifications: x400x in A and C, and x100 in B.
1746-1596-6-3-2.jpg
PMC3022785_02_1746-1596-6-3-2.jpg
F2
Most nuclei were small and round but focal nuclei were irregular and enlarged (Figure 2A). The outer cyst wall consisted of edematous fibrous tissue and scattered nests of luteinized cells that morphologically resembled the cyst lining cells (Figure 2B). Special stain showed reticulin fibers surrounding large nests of luteinized cells in the cyst lining (Figure 2C) and in the outer cyst wall.