PMC5712196_01
Scab symptoms caused by Venturia carpophila on a immature peach fruit, b a ripe peach fruit, c conidia of V. carpophila (400x), and d an 8-week old colony of V. carpophila growing on potato dextrose agar
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Fig1
Asexually produced conidia (Fig. 1c) of V. carpophila were scraped from a single lesion on the fruit surface using a scalpel, and a dilute spore solution prepared in sterile distilled water. A single germinated spore of V. carpophila was excised on an agar plug using a scalpel under a microscope (50x), and was transferred to antibiotic-amended potato dextrose agar, and was grown for 4 weeks under the same conditions described above (Fig. 1d). 
PMC5712196_02
Scab symptoms caused by Venturia carpophila on a immature peach fruit, b a ripe peach fruit, c conidia of V. carpophila (400x), and d an 8-week old colony of V. carpophila growing on potato dextrose agar
40793_2017_280_Fig1_HTML.jpg
PMC5712196_02_40793_2017_280_Fig1_HTML.jpg
Fig1
Asexually produced conidia (Fig. 1c) of V. carpophila were scraped from a single lesion on the fruit surface using a scalpel, and a dilute spore solution prepared in sterile distilled water. A single germinated spore of V. carpophila was excised on an agar plug using a scalpel under a microscope (50x), and was transferred to antibiotic-amended potato dextrose agar, and was grown for 4 weeks under the same conditions described above (Fig. 1d). 
The phylogenetic position of Venturia carpophila in comparison with other related fungal species. The tree was developed based on the 18S rRNA gene of the sequenced isolate of V. carpophila, an accession of the 18S rRNA gene of another isolate of V. carpophila, and accessions of other closely-related members of the family Venturiaceae (genera Fusicladium and Venturia) and with outgroup representatives (Phyllosticta harai, Staniwardia suttonii, Mycosphaerella graminicola, M. verrucosiafricana, Xenomeris raetica and Gibberella moniliformis, all in the class Dothidiomyectes of the phylum Ascomycota). The nearest neighbor joining tree was built by CLUSTALX2 and drawn to scale by TreeView based on 18S rRNA sequence phylogenetic analysis involved in a 1000-replicate bootstrapping process (numbers adjacent to branches are support values from 1000 bootstraps). The branch lengths in the tree were measured as substitutions per site, i.e., 0.1 on the scale bar representing 4 substitutions in 100 bp. The evolutionary history was inferred from 1522 aligned characters. The GenBank accession numbers for each species or strain are shown in parenthesis
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Fig2
The phylogenetic relationship of V. carpophila to other Ascomycota species based on the 18S rRNA genes shows that it is most closely related to members of the family Venturiaceae, particularly the genera Fusicladium and Venturia (Fig. 2). Based on the phylogenetic analysis using the sequence of the 18S rRNA gene (Fig. 2), V. carpophila is closely related to other scab-causing fungal pathogens of higher plants, including V. cerasi, causing scab on cherry, and also V. nashicola, cause of scab on Asian pear.
Count (a) and distribution (b) of microsatellites on scaffold_0 to scaffold_100. The total length of the 101 scaffolds (0 to 100) is 31,436,433 bp, accounting for 85.15% of the total genome size and containing 80.10% of the microsatellites. Scaffold_9 is the longest (1,454,817 bp) and has the most microsatellites (152). In b, each cross mark represents a microsatellite at the corresponding position on that scaffold
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Fig3
The count and distribution of microsatellites on scaffold_0 to scaffold-100 are presented (Fig. 3 a and b).