PMC4724101_01
Blood smear (May-Grunwald-Giemsa x1000). Arrow indicates inclusion bodies on erythrocyte surface
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Fig1
An EDTA-anticoagulated blood sample taken on admission revealed a markedly regenerative, severe (haematocrit 9.9 %; ref: 24-46 %) macrocytic anaemia with spherocytes and basophilic stippling present on the blood smear (Figs. 1 and 2) (Table 1).
Blood smear (May-Grunwald-Giemsa x1000). Polychromatic erythrocyte (arrow) and spherocytes (arrow heads)
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Fig2
An EDTA-anticoagulated blood sample taken on admission revealed a markedly regenerative, severe (haematocrit 9.9 %; ref: 24-46 %) macrocytic anaemia with spherocytes and basophilic stippling present on the blood smear (Figs. 1 and 2) (Table 1).
Spleen cut surface - the red pulp has a diffuse brick red to brownish discolouration and there is an increased prominence of lymphoid follicles in the white pulp (arrows)
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Fig3
Post mortem examination showed mild hepatomegaly, mild enlargement of the spleen with prominent lymphoid follicles within the white pulp (Fig. 3) and mild lymphadenomegaly of the right pre-scapular lymph node.
H&E section of lymph node showing EMH (this is an unusual location to find EMH). Both megakaryocytes (arrows) and nucleated erythroid precursors (arrowheads can be seen in clusters within the medullary cords
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Fig4
Numerous aggregates of nucleated erythroid precursors and variable numbers of megakaryocytes were noted in the medullary cords (Fig. 4).
H&E section of liver showing yellowish-brownish granules in the Kupffer cells
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Fig5
Increased intracytoplasmic accumulation of haemosiderin (confirmed by Perl's Prussian Blue staining) was also noted in Kupffer cells in hepatic sinusoids (Figs. 5 and 6).   
Perl's Prussian Blue staining showing haemosiderin pigments in the Kupffer cells (detected as a blue stain within Kupffer cells)
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Fig6
Increased intracytoplasmic accumulation of haemosiderin (confirmed by Perl's Prussian Blue staining) was also noted in Kupffer cells in hepatic sinusoids (Figs. 5 and 6).   