PMC9760960_01
PMC9760960_02
PMC9760960_03
CYP88L2 and CYP88L7 appear to hydroxylate cucurbitacin intermediates in metabolically engineered Cucurbita pepo hairy roots. (A) Total ion chromatograms of HPLC-QToF analysis from Cucurbita pepo hairy roots empty vector, CYP88L2 overexpression and CYP88L7 overexpression lines. The new peak at 12,65 min is indicated with an arrow. (B) MS2 for the ion 513.2779 [M+COOH]- mass spectra at minute 12.65 Cucurbita pepo overexpressing CYP88L2 and CYP88L7. Red diamonds represent ions detected in MS1 destined for fragmentation, while blue diamonds are these parental ion fragmented individually in the MS2 detector.
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PMC9760960_03_fpls-13-1021907-g007.jpg
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