PMC5662812_01
Sanger sequencing of BCR-ABL1 breakpoint demonstrating fusion of BCR exon 6 to ABL1 exon a2.
CRIHEM2017-9071702.001.jpg
PMC5662812_01_CRIHEM2017-9071702.001.jpg
fig1
RT-qPCR analysis with standardized primers and probes did not detect BCR-ABL1 transcripts but further analysis with a qualitative PCR approach detected a single abnormal band which Sanger sequencing demonstrated to be a fusion of BCR exon 6 to ABL1 exon a2 (Figure 1).
RT-qPCR of e6a2 BCR-ABL1 transcripts throughout clinical course. ASCT: allogeneic stem cell transplantation.
CRIHEM2017-9071702.002.jpg
PMC5662812_01_CRIHEM2017-9071702.002.jpg
fig2
Prior to ASCT the PB BCR-ABL1/ABL transcript level was 0.03% (Figure 2).