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Minimum spanning tree based on the cgMLST typing of the Norwegian VanD VREfm.. Regardless of the different STs, A1-3 strains clustered together while the VREfm strain B1 showed 354 allelic differences with the A2 strain.
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This was further shown by cgMLST analysis which revealed that A1-3 strains belonged to the same novel cluster type (CT) 3198 (Fig 1).
Phylogenetic SNP tree of the vanD gene clusters of the Norwegian and vanD1:vanD5 subtype reference clusters retrieved from NCBI.. Flags represent the countries that vanD-types were discovered in first. Case A strains clustered with vanD5 reference N03-0072 while case B strains clustered separately.
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A clustered with the vanD5 reference sequence (E. faecium strain N03-0072) (Fig 2). ANIs between the vanD5 reference sequence and A1-3 strains were >99.9%.