PMC6487761_01
Micro-capillary electrophoresis diagram. The decreased gammaglobuline zone is consistent with an hypogammaglobulinemia pattern.
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Serum globulin quantification was in agreement with those detected by micro-capillary electrophoresis diagram (Table 1 and Figure 1).
Thoracic radiographies show a mild bronchial pattern more evident in the peri-hilar lung field: (a) dorso-ventral view of the thorax and (b) right lateral view of the thorax.
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The radiographs of the thorax showed a mild diffuse bronchial pattern (Figure 2(a) and (b)).
Phylogenetic tree constructed from mtSSU rRNA sequences of Pneumocystis from dogs and 10 representative human and animal species with GenBank accession numbers as shown. Bootstrap values above 50% are indicated above the branches. The unroot tree was constructed using the Neighbor Joining method with 1000 Bootstrap replicates. Cavalier King in bold is from this study.
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The unroot phylogenetic tree constructed using mtSSU rRNA sequences showed that Pneumocystis carinii f. sp. canis was distinct from Pneumocystis spp. isolated from other animals, confirming that this was the specific species related to canine hosts (Figure 3).