PMC7607988_01
Three sets of three tubes as 1A-1B-1C; 2A-2B-2C; 3A-3B-3C. Set 1 and 2 had patient serum and Set 2 and 3 had fresh normal serum as source of complement. P1-positive cells were added to all nine tubes. Three sets "A," "B," and "C" were incubated at 4 C only, 37 C only, and 4 C followed by 37 C, respectively. After centrifugation, tube "2C" showed hemolysis leading to test being interpreted as positive
AJTS-14-57-g001.jpg
PMC7607988_01_AJTS-14-57-g001.jpg
F1
DAT with C3d specificity, treating physician and laboratory physician discussed and decided to perform DL test. DL test was positive, confirming PCH [Figure 1].