PMC3038955_01
Actinomadura meyerae colonies. A) A meyerae cultivated from our patient's bone biopsy on blood agar; B) Gram staining of an A. meyerae colony.
1752-1947-5-32-1.jpg
PMC3038955_01_1752-1947-5-32-1.jpg
F1
On the 28th day of culture, white colonies that were convex, powdery and adherent were detected on blood-enriched Columbia agar (Figure 1A). Gram staining demonstrated that the colonies were made of irregular and extensively branched Gram-positive bacilli less than 1 mum in diameter (Figure 1B).
Phylogenetic classification of the Actinomadura meyerae strain isolated from our patient's bone biopsy. Unrooted dendrogram showing the phylogenetic classification of validated Actinomadura species. The analysis was based on 16S rRNA sequence comparison using the neighbor-joining method and MEGA software. Bootstrap values greater than 70% are indicated at the nodes. The scale bar indicates a 0.2% sequence dissimilarity. GenBank accession numbers are indicated in parentheses.
1752-1947-5-32-2.jpg
PMC3038955_01_1752-1947-5-32-2.jpg
F2
Using the MEGA software, we performed a phylogenetic analysis among Actinomadura species and found our patient's strain to be grouped with A. meyerae (Figure 2).