PMC5100078_01
Surgical resection of the tumor. Tumor of 35 x 30 x 17 mm
12610_2016_41_Fig1_HTML.jpg
PMC5100078_01_12610_2016_41_Fig1_HTML.jpg
Fig1
Photo of the tumor at low magnification (X50). Nodular and well limited tumor composed of sheets of eosinophilic cells with Hematoxylin Eosin and Saffron (HES) stain
12610_2016_41_Fig2_HTML.jpg
PMC5100078_01_12610_2016_41_Fig2_HTML.jpg
Fig2
A (Ventana, prediluted, pretreatment: EDTA pH 7.8 for 60 min) and to SALL4 (Sigma, Dilution: 1/1500, pretreatment: EDTA pH 7.8 for 60 min). All tumor cells were diffusely and strongly stained with antibody to Inhibin A corresponding to Leydig cell tumor and were completely negative to antibodies to SALL4 showing that there was no germ cell contingent (Figs. 2, 3 and 4).   
Photo of the tumor at high magnification (X200). Tumor cells are large with an abundant eosinophilic cytoplasm and round regular nuclei with small nucleoli, according with Leydig cells. Hematoxylin Eosin and Saffron (HES) stain
12610_2016_41_Fig3_HTML.jpg
PMC5100078_01_12610_2016_41_Fig3_HTML.jpg
Fig3
A (Ventana, prediluted, pretreatment: EDTA pH 7.8 for 60 min) and to SALL4 (Sigma, Dilution: 1/1500, pretreatment: EDTA pH 7.8 for 60 min). All tumor cells were diffusely and strongly stained with antibody to Inhibin A corresponding to Leydig cell tumor and were completely negative to antibodies to SALL4 showing that there was no germ cell contingent (Figs. 2, 3 and 4).   
Photo of the tumor at high magnification (X400). Tumors cells were diffusely stained with antibody to inhibin A(immunoperoxydase). All tumor cells present a diffuse and strong cytoplasmic staining
12610_2016_41_Fig4_HTML.jpg
PMC5100078_01_12610_2016_41_Fig4_HTML.jpg
Fig4
A (Ventana, prediluted, pretreatment: EDTA pH 7.8 for 60 min) and to SALL4 (Sigma, Dilution: 1/1500, pretreatment: EDTA pH 7.8 for 60 min). All tumor cells were diffusely and strongly stained with antibody to Inhibin A corresponding to Leydig cell tumor and were completely negative to antibodies to SALL4 showing that there was no germ cell contingent (Figs. 2, 3 and 4).   
Photo of the testicular biopsy at high magnification (X100). Histopathological micrograph with hematoxylin-eosin-green FCF stain. The testicular biopsy consists of tubules with hypospermatogenesis (white arrows) mixed with aplasia (black arrows)
12610_2016_41_Fig5_HTML.jpg
PMC5100078_01_12610_2016_41_Fig5_HTML.jpg
Fig5
Testicular Steroidogenesis
12610_2016_41_Fig6_HTML.jpg
PMC5100078_01_12610_2016_41_Fig6_HTML.jpg
Fig6