PMC3898211_01
Capillary zone electrophoresis of the patient's hemoglobin. CE shows the hemoglobin variant eluting with normal hemoglobins. Note peaks corresponding to Hb Shepherds Bush (6.5% of total, zone 10), HbA (89.6%, zone 9), HbF (0.3%, zone 7), HbA2 (3.2%, zone 3) and hemoglobin breakdown products (0.4%, zone 2). This chromatogram was obtained after a transfusion following a parvovirus infection described later accounting for the small amount of her native hemoglobin compared to HbA.
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F1
A CE chromatogram performed at a later date after transfusion (Figure 1) is included to show the migration pattern of our patient's hemoglobin variant, which eluted slightly earlier than HbA.
High performance liquid chromatography analysis of the patient's hemoglobin. HPLC analysis shows a peak of unknown variety at 2.13 min that eluted earlier than HbA at 2.44 min. The chromatogram also contains peaks corresponding to HbF and HbA2.
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F2
Using high performance liquid chromatography (HPLC) for hemoglobin (Figure 2), several hemoglobin types were demonstrated.
Alkaline hemoglobin electrophoresis of the patient's hemoglobin. Alkaline hemoglobin electrophoresis on cellulose acetate with densitometer tracing superimposed below electrophoresis bands shows a dense band where HbA is expected to appear and a second band that has migrated more anodally indicating an unknown hemoglobin variant.
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F3
Alkaline gel electrophoresis was also utilized to demonstrate the migration of the unknown hemoglobin variant, which migrated slightly more anodally than HbA (Figure 3).