PMC10365966_01
Serum lactate dehydrogenase (LD) levels and tumor volume reduction associated with the treatment. (A) The transition of serum LD levels was thought to reflect the disease state. Moreover, computed tomography (CT) scans before treatment and after the two courses showed the tumor. (B) The serum LD levels decreased with initiation of treatment. With decreased LD levels, multiple liver and lung metastases also significantly reduced after the two courses.
fonc-13-1231986-g001.jpg
PMC10365966_01_fonc-13-1231986-g001.jpg
f1
Her serum lactate dehydrogenase (LD) levels were used to monitor the disease state, as shown in  Figure 1 .
Liver biopsy tissue. HE staining: increased necrotic area after treatment. (A) Pretreatment. (B) After two courses. IHC: P16 was positive. Local lymphocyte infiltration was observed before the initiation of treatment. (C) P16 was positive (D) CD8+ T lymphocytes pretreatment. (E) CD8+ T lymphocytes after two courses. Lymphocyte accumulation was observed around the liver. HE, hematoxylin and eosin; IHC, immunohistochemistry.
fonc-13-1231986-g002.jpg
PMC10365966_01_fonc-13-1231986-g002.jpg
f2
A liver biopsy before treatment showed CD8+ T cell infiltration in the tumor ( Figure 2D ), and p16 was positive ( Figure 2C ). A second liver biopsy showed increased tumor necrosis ( Figures 2A, B ), while the number of tumor-infiltrating CD8 T cells remained the same as in the previous biopsy ( Figure 2E ).