PMC8495192_01
Clinical time course of the patient.
fped-09-725940-g0001.jpg
PMC8495192_01_fped-09-725940-g0001.jpg
F1
A brief summary has been shown in the timeline (shown in Figure 1).
Agarose gel electrophoresis patterns of the PCR-based open reading frame typing (POT) using the Cica Geneus Pseudo POT KIT. PCR of reaction mixtures 1 and 2 was carried out for strain 1 and 2. Lane 1: strain 1 obtained from the patient's cerebrospinal fluid. Lane 2: strain 2 obtained from a cleaning sponge brush. The sizes of the bands in the M lane are 600, 500, 450, 400, 350, 300, 250, 200, 150, and 100 bp (from the top). PC, positive control; NC, negative control, used as an in-house ladder marker for detection PCR of Pseudomonas aeruginosa. Lines 1 and 2 showed the same band patterns, and the POT numbers of both strain 1 and 2 were the same values. The results from POT 1 and 2 values indicated these strains did not carry a metallo-beta-lactamase, such as IMP and VIM.
fped-09-725940-g0002.jpg
PMC8495192_01_fped-09-725940-g0002.jpg
F2
Pseudomonas aeruginosa was isolated from a cleaning sponge brush and had the same band pattern as the isolate obtained from CSF culture as shown by agarose gel electrophoresis patterns of the PCR-based open reading frame typing (POT) (shown in Figure 2).