PMC3535203_01
Detection of the minor BCR-ABL fusion transcript at the lymphoblastic crisis phase by real-time PCR. The amplification curves for the minor BCR-ABL fusion transcript (A) and ABL1 gene (B) are shown. The ratio of the minor BCR-ABL fusion transcript was calculated as 1.17 normalized copy number (NCN).
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F1
Ratios of 166.42 (converted to international scale-normalized copy number, IS-NCN) and 1.17 (NCN) were obtained for the major and minor transcripts, respectively (Fig. 1).
Analysis of RNA samples collected from the chronic and lymphoblastic crisis phase of CML using the HemaVision screening and split-out kits (Bio-Rad Laboratories). The screening kit produced a single band in the M6 lane (A) whereas the spilt-out kit produced a single 397-base pair band in the M6B lane (B), indicating the presence of the major BCR/ABL fusion transcript (b2a2 type) during the chronic phase. However, during the lymphoblastic crisis phase, the screening kit produced 2 bands in lanes M6 and M8 (C) and the spilt-out kit produced a 397-base pair band in the M6B lane (D) and a 320-base pair band in the M8F lane (E). This indicates the simultaneous presence of both the major (M6B, b2a2 type) and minor (M8F, e1a2 type) BCR/ABL fusion transcripts in this patient.
alm-33-80-g002.jpg
PMC3535203_01_alm-33-80-g002.jpg
F2
Only the major BCR/ABL fusion transcript was detected in the chronic phase sample (Fig. 2A and B). Notably however, both the major and minor BCR/ABL fusion transcripts were detected in the LBC phase sample (Fig. 2C-E).