PMC5291559_01
(A) Modified gomori trichrome staining indicated the red nemalines scatter in muscle fibers. (B) Immunostaining indicated that the nemalines in muscle fibers were alpha-actinin positive. Magnification: 200x.
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F1
Modified gomori trichrome staining revealed numerous red rod-shaped bodies accumulated in 7% muscle fibers (Figure 1A). Immunostaining showed that the rod-shaped bodies had alpha-actinin positive reaction (Figure 1B).
Electron microscopy showed that muscle fibers contained many arrays of osmiophilic oval or rectangular structures. Magnification: 5000x.
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F2
These structures mainly scattered in the sarcoplasm, occasionally distributed under subsarcolemma (Figure 2).