PMC5796964_01
(A) Basophil activation test. The rate of active basophil expressing CD63 in patients 1, 2, and 3 was 63.24%, 75.64%, and 24.07%. P1, 2, and 3: patient 1, 2 and 3. (B) Separation on 4%-12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analysis of immunoblot. Lane 1 was protein marker. With Coomassie brilliant blue stain, more than 11 bands were observed in the total extract of boiled yam (lane 2). Immunoblot with sera from patients 1, 2, and 3 (lanes 3, 4, and 5) detected a 30-kDa IgE binding band. A 17-kDa protein was recognized by patient 3 (lane 5).
apa-8-e4-g001.jpg
PMC5796964_01_apa-8-e4-g001.jpg
F1
By the immunoblotting, a protein band with molecular mass around 30 kDa was recognized by all the patients' sera (Fig. 1B), but by none of the nonatopic controls.