PMC4095839_01
Temporal sequence of patient's sepsis, from hospital admission to haploidentical HSCT, with detailed clinical and laboratory steps.HSCT = hematopoietic stem cell transplantation; CT = computerized tomography; BAL = bronchoalveolar lavage; GM = galactomannan.
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F001
Concomitantly to the other microbiologic analyses, SeptiFast was performed and resulted positive for Aspergillus fumigatus (Figure 1); the positive result was available after only 4 hours from patient's admission, and importantly was confirmed in three consecutive samples of patient's peripheral blood.
Chest CT scan of the patient shows a large consolidation area and peripheral ground-glass opacities in left lower lobe. CT = computerized tomography.
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F002
The following day a computerized tomography (CT) scan of the chest (Figure 2) was performed, evidencing an extensive lobar pneumonia, highly suggestive for fungal infection.
Cytology on bronchoalveolar lavage: septate hyphae, suggestive for Aspergillus, and foamy macrophages.
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Chest CT scan of the patient, performed 10 days after starting of the targeted antifungal therapy, showed a marked reduction of the lobar pneumonia and particularly of the ground-glass opacities. CT = computerized tomography.
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F004
A CT scan (Figure 4) performed 10 days after starting voriconazole showed a marked reduction of the lobar pneumonia and ground-glass opacities.
Immune reconstitution after allogeneic HSCT: absolute counts of lymphocyte subpopulations of CD3+, CD4+ and CD8+ T cells, assessed by whole blood immunophenotypic staining with specific monoclonal antibodies (all from Biolegend) and Flow-Count Fluorospheres (Beckman Coulter), acquired using a Gallios cytometer and analyzed with Kaluza software (all from Beckman Coulter). HSCT = hematopoietic stem cell transplantation; GvHD = graft versus host disease.
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