PMC4999642_01
Patient's venous blood is drawn
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F1
10 ml of patients venous blood is drawn (Fig. 1) and collected in sterile plastic vaccutube coated with anti-coagulant EDTA (Fig. 2).
Centrifugal machine
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F3
Centrifugation machine (Fig. 3) is used for obtaining PRP with a speed of 1000 rpm for 10 min.
Three layers of PRP
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F4
After centrifugation three layers are obtained (Fig. 4).
Aspiration of plasma and RBC
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F5
The straw colored plasma and RBC is aspirated and collected in other test tube (Fig. 5), and again centrifugated for 10 min and PRP obtained is placed in sterile test tube.
Mixed with calcium chloride
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F6
For activation PRP is mixed with calcium chloride (Fig. 6).
Pulpotomy with PRP
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F7
A thick mix of ZOE was placed to seal the coronal pulp chamber (Fig. 7).
Postoperative after 15 days
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F8
Clinical examination was undertaken at 15 days (Fig. 8), 1st and 3rd month intervals, where as radiographic evaluation of the treated teeth were carried out at 1st and 3rd month interval (Fig. 9).
PMC4999642_02
Extraction socket
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F10
PRP was placed in extraction socket immediately after extraction and betadiene pack was given (Figs 10 and 11). Post extraction instructions were given and patient was recalled after 3 days.
Application of PRP in extraction socket
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F11
PRP was placed in extraction socket immediately after extraction and betadiene pack was given (Figs 10 and 11). Post extraction instructions were given and patient was recalled after 3 days.
After 3 days (approximation of flaps)
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F12
Clinical examination was done anc it shows rapid healing of socket (Fig. 12).