PMC4208431_01
Histology and electron microscopy. (a) Normal liver lobule with pale hepatocytes and ceroid pigments (CP) at their apical pole (H&E staining, original magnification x400). (b) Diffuse clarification of hepatocytes in relation with marked glycogen reduction (PAS staining). (c) Electron microscopic appearance of a typical hepatocyte showing glycogen deficit (white areas). (d) Overview of the cytoplasm of a typical hepatocyte at electron microscopy. Low density of rough endoplasmic reticulum, mitochondria, and primary lysosomes. Increased number and hyperplasia of smooth endoplasmic reticulum (SER) and Golgi (G) apparatus (white box). Secondary lysosomes (SL) considered in that case as autophagosomes.
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fig1
In addition, ceroid pigments at the apical pole of the hepatocytes were observed (Figure 1). This observation contrasts with an increased number of Golgi bodies and smooth endoplasmic reticulum as well as primary and secondary lysosomes containing digested material (Figure 1).