PMC6369487_01
Clinical aspect of the lesion of the anal margin.
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fig1
Microscopic aspect on biopsy, revealing moderately differentiated squamous cell carcinoma (a), with irregular/heterogenous positivity for p16 immunohistochemistry (b).
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fig2
Using immunohistochemistry, irregular/heterogenous positivity for p16 protein was observed (Figure 2).
Microscopic and immunohistochemical aspects of 1st excision. Tumor consisted of nests of invasive squamous cell carcinoma, with focal keratinization (a). p53 appeared continuous and limited to the periphery of invasive nests, with strong intensity (b).
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fig3
Immunoreactivity of p53 (clone DO-7, 1:200, Dako Agilent) appeared continuous and limited to the periphery of invasive nests, with strong intensity (Figure 3).
Microscopic and immunohistochemical aspects of 2nd excision. At low power view, the epithelium appeared thickened with parakeratosis and elongated rete ridges (a). At high power view, note the disorderly basal cell layer, prominent intercellular bridges, prominent nucleoli, and mitosis (b). Immunoreactivity of p53 appeared limited to the basal and suprabasal layers of the epithelium, with weak to moderate intensity (c). Immunoreactivity of p53 in adjacent "normal" epithelium (d).
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fig4
Immunoreactivity of p53 appeared limited to the basal and suprabasal layers of the epithelium, with weak to moderate intensity (Figure 4).