PMC9978506_01
Clinical presentation of the dog with suspected PBL. (A) Cervical mass on the right side. (B, C) Right oral/neck mass was very large (approximately 15 x 10 cm).
fvets-10-1100942-g0001.jpg
PMC9978506_01_fvets-10-1100942-g0001.jpg
F1
The right side of the face was significantly swollen and a clear distinction between the oral mass and neck mass was difficult (Figures 1A-C).
Soft tissue window computed tomographic images. (A) (left) and (B) (right): in (A) dorsal and (B) transversal planes oriented with the patient's right side on the left of the image. Mass lesion (white arrows) arising from the lateral margin of the right mandibular salivary gland**. The left mandibular salivary gland (MSG) is labeled for comparison. (C) (left), (D) (right): Soft tissue window computed tomographic images in the transverse plane, oriented with the patient's right side on the left of the image. Images show multiple mass lesions arising from the (C) subcutaneous tissues of the right aspect of the neck (white arrows), (D) poorly defined hyper-contrast enhancing mass lesions in the musculature deep to the scapulae bilaterally (white oval and black arrows) and more well-defined mass lesions within the dorsal, left subcutaneous tissues (dashed white arrows).
fvets-10-1100942-g0002.jpg
PMC9978506_01_fvets-10-1100942-g0002.jpg
F2
The mandibular and retropharyngeal lymph nodes were normal in size (Figures 2A, B). No osteolytic lesions were noticed in any of the bones (Figures 2C, D). Neoplastic cells were rounded with often indistinct cytoplasmic borders, clear eosinophilic cytoplasm, oval to reniform and sometimes eccentric nuclei, dispersed to condensed chromatin, single central nucleolus, marked anisokaryosis, moderate nuclear pleomorphism, and 40-60 mitotic figures in 10 400 x fields (2.37 mm2).
Photomicrograph of the sample of PBL in the dog. Histopathology of neoplasm, (A) high cellularity, no encapsulation, and infiltration. 4x, H&E. (B) Round cells, eosinophilic cytoplasm, oval nucleus, and eventually reniform. Chromatin-dispersed single central nucleolus and marked anisokaryosis. Many mitosis figures (arrow), 40x H&E. Immunohistochemistry, (C) MUM1, Neoplastic cells showed strong nuclear staining. (D) CD79 immunohistochemistry. Some neoplastic cells, 40x (< 20%) showed strong cytoplasmic staining, 40x.
fvets-10-1100942-g0003.jpg
PMC9978506_01_fvets-10-1100942-g0003.jpg
F3
No difference in microscopic appearance from the previous histopathology was reported (Figures 3A, B). MUM-1 (for plasma cell differentiation) staining was positive with moderate-to-strong immunoreactivity and approximately 90% of positive neoplastic cells, and CD79a was also scantly positive (Figures 3C, D).