PMC5278071_01
Histopathological section of the liver showing: A) Hepatocytes with clear and fine granular cytoplasm (white arrow) (hematoxylin and eosin stain x400), B) Periodic acid-Schiff (PAS) stain showing large amount of dark red-colored glycogen in hepatocytes (PAS stain x200), and C) Complete digestion of glycogen after adding diastase stain and reappearance of clear hepatocytes (white arrow) (PAS stain and diastase x400).
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F1
Additionally, numerous hepatocytes exhibited glycogenized nuclei and abundant periodic acid-Schiff (PAS) stain-positive cytoplasmic glycogen deposits, which were not visible after digestion with diastase to confirm glycogen deposition (Figure 1A-C).