PMC4327548_01
a Red, pigmented plaque on the back, 90 x 80 mm, with a dome-shaped elastic-soft nodule. b The squamous cell-composed tumor was surrounded by another type of tumor. c Center of the tumor (squamous cell-composing tumor area): the atypical squamous cells contained large oval nuclei and occasionally conspicuous nucleoli, with individual keratinization, mitotic figures and clumping cells. d Peripheral areas of the tumor: small round cells with scant cytoplasm and oval nuclei. Original magnification: b x50; c, d x200.
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F1
On his initial visit, physical examination revealed a red, pigmented plaque, 90 x 80 mm, with a dome-shaped elastic-soft nodule (fig. 1a). In addition, the biopsy specimen from the center of the nodule revealed dense infiltrating atypical squamous cells throughout the dermis (fig. 1c). Unexpectedly the histological finding of the whole tumor revealed that the squamous cell-composed tumor was surrounded by another type of tumor composed of small round cells with scant cytoplasm and oval nuclei (fig. 1b, d).
CD8+ cells and caspase 3+ cells in the areas of invasive BD and MCC. Paraffin-embedded tissue samples were deparaffinized and stained with anti-CD8 antibody (a, b) and anti-caspase 3 antibody (c, d) for the areas of invasive BD (a, c) and MCC (b, d). The sections were developed with liquid permanent red. Original magnification: x200.
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F2
Therefore, we further employed immunohistochemical staining for CD8 (fig. 2a, b), caspase 3 (fig. 2c, d), Foxp3 (fig. 3a, b), CD163 (fig. 3c, d) and CD206 (fig. 3e, f) in the lesional areas of invasive BD and MCC.
Foxp3+ Tregs, CD163+ macrophages and CD206+ cells in the areas of invasive BD and MCC. Paraffin-embedded tissue samples were deparaffinized and stained with anti-Foxp3 antibody (a, b), anti-CD163 antibody (c, d) and anti-CD206 antibody (e, f) for the areas of invasive BD (a, c, e) and MCC (b, d, f). The sections were developed with liquid permanent red. Original magnification: x200.
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PMC4327548_01_cde-0007-0001-g03.jpg
F3
Therefore, we further employed immunohistochemical staining for CD8 (fig. 2a, b), caspase 3 (fig. 2c, d), Foxp3 (fig. 3a, b), CD163 (fig. 3c, d) and CD206 (fig. 3e, f) in the lesional areas of invasive BD and MCC.