PMC7787855_01
(a) CT scan (transverse view) revealing hydatid cyst in superior and inferior segment of the lower lobe of the right and left lungs, respectively. (b) CT scan (frontal view) indicates hydatid cyst in superior and inferior segment of the lower lobe of the right and left lungs, respectively. (c) Fungal hyphae on the outer chitinous wall of the hydatid cyst in the right lung (H&E x100). (d) Photomicrograph showing groups of branching septate hyphae conforming to morphology of Aspergillus in hydatid cyst tissue in the right lung (H&E x400).
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fig1
Surprisingly, the ectocyst in the right lung showed the presence of numerous clusters of Aspergillus septate hyphae with acute-angled branching in hematoxylin and eosin stain (H&E) (Figures 1(c) and 1(d)).
Agarose gel electrophoresis of the seminested PCR products of the ITS1-5.8s-ITS2 region. Line M, 100 bp molecular marker; lines 1 and 2, positive control (Aspergillus niger); line 3, sample isolated from the patient in the current study; line 4, 1/10 diluted patient sample (Aspergillus niger); line 5, negative control.
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fig2
The amplified products were visualized, using 1.5% agarose gel containing 0.5 mug/ml ethidium bromide stain (Figure 2).