PMC6119272_01
Summarizing scheme of disease progress
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Fig1
Ophthalmoscopy of the right eye revealed papillary edema, retinal hemorrhages (Roth's spots) and arteriovenous nickings (for further details see Fig. 1 and Table 1).
Bone marrow smears of an acute myeloid leukemia without maturation case showing numerous blasts with round nuclei, fine nuclear chromatin, and dark blue cytoplasm (Leishman stain, oil immersion x 100)
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Fig2
Bone marrow (BM) aspiration revealed 70% of blasts (Fig. 2).
GTG-banding revealed a hyperdiploid karyotype multiple numerical and or structural rearrangements
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Fig3
48-50,X,- X,der(1)t(1;2)(?;?),der(1)t(1;3)(?;?),+ 4,+ 4,+ 4,+ 6,t(8;11)(?;?),t(10;12)(?;?),dic(12;17)(?;?)x 2 (Fig. 3), which was further specified by molecular cytogenetic studies (Figs. 4 and 5).
aCGH characterized losses in 17p13.3-17p11.2 and 17q11.2-17q11.2 regions and gains in 17p11.2-17p11.1, 17q11.1-17q11.2 and 17q11.2-17q12.2 regions. These observations were compatible with the FISH results and their locations according to the Genome Reference Consortium human genome (build 37) (GRCh37)/Human Genome Issue 19 (available from https://genome.ucsc.edu)
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Fig6
The aCGH analysis revealed different genomic imbalances (Fig. 6).
GTG-banding in secondary AML-M6 revealed a tetraploid karyotype in 20% of the analyzed cells
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Fig7
GTG-banding secondary AML-M6 revealed a hyperdiploid karyotype in 10% of the analyzed cells
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Fig8