PMC4888639_01
Combined HCC/CCC in a 72 year old male patient with history of hepatitis C and diabetes revealing morphological, immunohistochemical and genetic intratumor heterogeneity. a Gross morphology and slide overview (H&E staining) with annotations of defined tumor areas. b Microscopic images (40x) of different tumor areas (H&E staining, CK7, glutamine synthetase and beta-catenin immunohistochemistry). c p53 and beta-catenin nuclear positivity in consecutive slides of tumor area A2. d Nucleotide exchanges in CTNNB1 and TP53 genes (TP53 depicted as reverse sequence). e Mutated allele frequencies of double mutated areas A1-3 with number of gene copies analyzed. Reference sequences: NM 1904.1 (CTNNB1) and NM 001126112.2 (TP53)
12907_2016_29_Fig1_HTML.jpg
PMC4888639_01_12907_2016_29_Fig1_HTML.jpg
Fig1
Table 1 and Fig. 1 illustrate histopathological and molecular findings in 14 individual tumor areas, which were grouped into three tumor regions (A, B and C) according to their predominant morphological and molecular characteristics. A1-3 (Fig. 1). All mutated and wild type tumor areas additionally displayed a SNP of exon 7 (rs17880604).