PMC4272953_01
PMC4272953_02
Karyotypic findings in the patient and donor. Conventional cytogenetic analysis from the patient's bone marrow before transplantation (A) and in the peripheral blood after transplantation (B) demonstrated a karyotype of 46,XY,t(2;11)(q32;q23). Conventional cytogenetic analysis from the sibling donor's peripheral blood showed a karyotype of 46,XY,t(2;11)(q32;q23) (C). Mononuclear cells from the sibling donor's peripheral blood showed a constitutional chromosomal aberration of 46,XY,t(2;11)(q32;q23), which was also confirmed by M-FISH analysis (D). Arrows indicate the aberrant chromosomes.
alm-35-162-g001.jpg
PMC4272953_02_alm-35-162-g001.jpg
F1
Conventional cytogenetic analysis of the BM aspirate revealed a complex abnormality: 6 out of 20 metaphases were inappropriate, and the karyotype at initial diagnosis of AMKL was 45, XY,-22,t(2;11)(q32;q23),der(14)t(14;22)[6]/46,XY,t(2;11)(q32;q23)[8] (Fig. 1A). However, cytogenetic analysis of the BM aspirate still demonstrated the t(2;11)(q32;q23) abnormality (Fig. 1B). M-FISH from peripheral blood (PB) of the donor. Mononuclear cells from the PB of the sibling donor were determined to have a constitutional chromosomal aberration of 46,XY,t(2;11)(q32;q23) [20] (Fig. 1C, D).