PMC9958271_01
PMC9958271_02
Detection of spirochetes by immunohistochemistry in vulvar skin biopsy from the 14-month-old girl. Light microscopy of the vulva squamous epithelium showing an inflammatory infiltrate containing numerous spirochetes. The slide was labeled using anti-Treponema antibody (Biocare) with a red alkaline phosphatase revelation, and hematoxylin counter staining (X40 magnification).
gr1.jpg
PMC9958271_02_gr1.jpg
fig0005
Syphilis reference center for analysis. No evidence of Langerhans cell histiocytosis was found. Spirochete immunostaining revealed the presence of multiple spirochetes invading the epidermis (Fig. 1), and the Treponema pallidum-specific PCR on the tissue was positive.
X-ray of long bones. X-ray of lower limbs with arrows showing multiple periosteal appositions, alternating radiolucent and radiodense metaphyseal lines, and osteolytic lesions.
gr2.jpg
PMC9958271_02_gr2.jpg
fig0010
The X-ray of long bones found features suggestive of syphilis: multiple periosteal appositions, alternating radiolucent and radiodense metaphyseal lines, and osteolytic lesions (Fig. 2).
PMC9958271_03