PMC3884168_01
Clinical image and histopathologic analysis of intratarsal keratinous cysts. a High-power view of the cyst described in case 1 stained with hematoxylin and eosin (H&E). b Clinical photograph of the intratarsal keratinous cyst in the left upper eyelid described in case 2. c Low-power view of the cyst described in case 2, stained with H&E, highlighting its intratarsal location. d Higher magnification view of the cyst in case 2, stained with H&E. e AE1/AE3 is expressed in the apical epithelium as well as in the keratin filling. f CK 903 is expressed throughout the cyst epithelium and keratin. g CK 5/6 expression is faintly detected within the cyst epithelium and keratin. h CEA is expressed in the cyst cuticle and keratin.
cop-0004-0160-g01.jpg
PMC3884168_02_cop-0004-0160-g01.jpg
F1
On eversion of the left upper eyelid, there was a small, white, well-circumscribed, nodular lesion in the tarsus with inflammation of the surrounding conjunctiva (fig. 1b). The epithelium lacked goblet cells and sebaceous cells; however, there were few clear cells present (fig. 1c, d). Immunohistochemistry revealed expression of pancytokeratin (AE1/AE3) in the cyst cuticle and lining (fig. 1e). In addition, there was expression of cytokeratin (CK) 903 in the epithelium, cuticle, and contents, and expression of CK 5/6 in the cyst lining and contents, but not cuticle (fig. 1f, g; table 1). The sloughed keratinous material within the cyst strongly expressed CEA (fig. 1h), but we did not identify staining with this antibody in the normal Meibomian gland.
PMC3884168_02
Clinical image and histopathologic analysis of intratarsal keratinous cysts. a High-power view of the cyst described in case 1 stained with hematoxylin and eosin (H&E). b Clinical photograph of the intratarsal keratinous cyst in the left upper eyelid described in case 2. c Low-power view of the cyst described in case 2, stained with H&E, highlighting its intratarsal location. d Higher magnification view of the cyst in case 2, stained with H&E. e AE1/AE3 is expressed in the apical epithelium as well as in the keratin filling. f CK 903 is expressed throughout the cyst epithelium and keratin. g CK 5/6 expression is faintly detected within the cyst epithelium and keratin. h CEA is expressed in the cyst cuticle and keratin.
cop-0004-0160-g01.jpg
PMC3884168_02_cop-0004-0160-g01.jpg
F1
On eversion of the left upper eyelid, there was a small, white, well-circumscribed, nodular lesion in the tarsus with inflammation of the surrounding conjunctiva (fig. 1b). The epithelium lacked goblet cells and sebaceous cells; however, there were few clear cells present (fig. 1c, d). Immunohistochemistry revealed expression of pancytokeratin (AE1/AE3) in the cyst cuticle and lining (fig. 1e). In addition, there was expression of cytokeratin (CK) 903 in the epithelium, cuticle, and contents, and expression of CK 5/6 in the cyst lining and contents, but not cuticle (fig. 1f, g; table 1). The sloughed keratinous material within the cyst strongly expressed CEA (fig. 1h), but we did not identify staining with this antibody in the normal Meibomian gland.