PMC5109575_01
Clinical course.
1349-7235-55-3035-g001.jpg
PMC5109575_01_1349-7235-55-3035-g001.jpg
g001
Her platelet count gradually increased and was 13.3x104/muL at 8 days after drug discontinuation (Fig. 1). The antibody titer (mean fluorescence intensity) was decreased after cessation of vancomycin and undetectable as the platelet count raised (Fig. 1).
Histograms of fluorescence intensities of platelet-bound IgG antibody. Black: The patient's serum was tested against normal washed platelets in the absence of vancomycin. White: The patient's serum was tested against normal washed platelets in the presence of vancomycin. Gray: Normal serum was tested against normal washed platelets in the presence of vancomycin as a negative control.
1349-7235-55-3035-g002.jpg
PMC5109575_01_1349-7235-55-3035-g002.jpg
g002
The result of the flow cytometric analysis is shown in Fig. 2.
platelet antigen recognized by vancomycin-dependent antibody. Antibody binding in the patient's serum and normal serum to each platelet glycoprotein was measured using a pakAuto assay kit. Specific binding was expressed as % of control calculated using the following formula: % of control (%)=(sample - negative control) / (positive control - negative control)x100. Negative and positive controls were included in the kit.
1349-7235-55-3035-g003.jpg
PMC5109575_01_1349-7235-55-3035-g003.jpg
g003
We found that the antibody in the patient's serum specifically recognized GPIb/IX (Fig. 3).