PMC7090362_01
A) This wet preparation mount is showing sporangiophore containing terminal sporangia which is forcibly discharged, a characteristic feature observed in Basidiobolus species. B) This lactophenol cotton blue stain is showing the same sporangiophore. C) Lactophenol cotton blue stain demonstrating the characteristic beak-like appendage of a thick-walled zygospore. D) Conidia may be primary or secondary.
gr1.jpg
PMC7090362_01_gr1.jpg
fig0005
Microscopic examination showed large irregular, non-septate hyphae with numerous smooth, round, thick- walled zygospores possessing a distinct beak-like appendage characteristic of Basidiobolus species (Fig. 1.A, B, C).
A and B) Histopathology with H&E stain is demonstrating the Splendore-Hoeppli phenomenon which is describing the intense eosinophilic inflammatory surrounding the ribbon-like hyphae which are sparsely septated.
gr2.jpg
PMC7090362_01_gr2.jpg
fig0010
Histopathology revealed characteristic Splendore-Hoeppli phenomenon on haematoxylin and eosin (H&E) staining, consisting of granulation tissue surrounding short or long, broad, ribbon-type sparsely septated hyphae, surrounded by eosinophils, lymphocytes and giant cells (Fig. 2.A-B).