PMC3804825_01
Histological and immunohistochemical features of thyroid nodule. A) Low power view of the nodule demonstrating the abundant mature adipose tissue. B) Higher magnification of lesion showing papillary architecture and nuclear overlapping. C) Immunostaining with Gal-3 showing the atypical positive cells (center of the field). D) Note the plasma membrane reaction with HBME-1 of atypical cells (right) compared with the negative immunoreaction of other bland cells (left).
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Microscopically two colloid-cystic nodules showed papillary structures with a delicate stroma and prominent intralesional mature fat (Figure 1A). The nuclei in fact were sometimes enlarged, with scattered grooves, overlapping and clearing, but no nuclear pseudoinclusion or psammomabodies were found (Figure 1B). Interestingly galectin-3 expression was restricted to those cells showing atypical cytological features whereas HBME-1 immunostaining further highlighted the plasma membrane aspect of these atypical cells (Figure 1C and D) However the larger part of the papillary lesion was unreactive with both Galectin-3 and HBME-1.