PMC3916506_01
Preoperative view of discoloured maxillary lateral incisor.
rde-39-51-g001.jpg
PMC3916506_01_rde-39-51-g001.jpg
F1
A 35 year old male patient reported to the Department of Periodontics with a complaint of pain in the upper left front tooth region associated with pus discharge for 2 months (Figure 1).
Preoperative radiograph showing radiolucency along distal aspect of maxillary lateral incisor to the apex.
rde-39-51-g002.jpg
PMC3916506_01_rde-39-51-g002.jpg
F2
Intraoral periapical X-ray (IOPA) was taken which revealed radiolucency along the distal side of the entire root surface to the apex (Figure 2).
Separation of platelet rich plasma from the collected autologous blood.
rde-39-51-g003.jpg
PMC3916506_01_rde-39-51-g003.jpg
F3
Whole blood was initially centrifuged (3,000 rpm for 10 minutes, Figure 3).The plasma was then aspirated with a micropipette and subjected to a second centrifugation at 2,000 rpm for 10 minutes, which allowed the precipitation of the platelets (0.8 to 1.2 mL) to fall onto the bottom.
Platelet rich fibrin after activation with calcium chloride.
rde-39-51-g004.jpg
PMC3916506_01_rde-39-51-g004.jpg
F4
Coagulated preparation of 0.3 mL of PRF was obtained by its combination with 0.1 g of calcium chloride (Figure 4).
Curettage of the defect after reflection of soft tissue flap using intracrevicular incision.
rde-39-51-g005.jpg
PMC3916506_01_rde-39-51-g005.jpg
F5
A full thickness flap was raised at the labial aspect using intracrevicular incision (Figure 5).
Placement of graft into defect.
rde-39-51-g006.jpg
PMC3916506_01_rde-39-51-g006.jpg
F6
Thorough curettage of the defect area was done and a combination of PRF and bioactive glass was placed in the defect (Figures 6 and 7).
Placement of PRF.
rde-39-51-g007.jpg
PMC3916506_01_rde-39-51-g007.jpg
F7
Thorough curettage of the defect area was done and a combination of PRF and bioactive glass was placed in the defect (Figures 6 and 7).
Follow-up radiographs (a) at 6 months, postoperative view showing bone fill; (b) at 12 months.
rde-39-51-g008.jpg
PMC3916506_01_rde-39-51-g008.jpg
F8
PRF and bone graft resulted in substantial amount of bone fill (Figures 8a and 8b), and probing depth was reduced to 2 mm.