PMC1402319_01
Aspirate from the EUS-FNA of lymph node revealed small to intermediate sized mononuclear cells with moderate cytoplasm, round to oval nuclei with smooth nuclear borders, a stippled chromatin pattern and occasional single nucleoli. The cytoplasm is pale grey with hair-like and short, blunt, cytoplasmic projections. (Stain: Diff Quik, Magnification: x 40).
1742-6413-3-1-1.jpg
PMC1402319_01_1742-6413-3-1-1.jpg
F1
The cytoplasm was pale grey and granular with hair-like and short, blunt, cytoplasmic projections (cytoplasmic blebs) (figures 1, 2).
A higher magnification of the aspirate from same area as shown in Figure 1 shows small to intermediate sized mononuclear cells with moderate cytoplasm, round to oval nuclei with smooth nuclear borders, a stippled chromatin pattern and occasional single nucleoli. The cytoplasm is pale grey and granular with hair-like and short, blunt, cytoplasmic projections. (Stain: Diff Quik, Magnification: x 60).
1742-6413-3-1-2.jpg
PMC1402319_01_1742-6413-3-1-2.jpg
F2
The cytoplasm was pale grey and granular with hair-like and short, blunt, cytoplasmic projections (cytoplasmic blebs) (figures 1, 2).
Immunophenotyping performed using selective gating on lymphoid cell population shows that neoplastic cells are positive for clonal B-cells (>98%) expressing CD19, CD20, CD11c bright, CD25 bright, CD103. surface immunoglobulin and are lambda light chain restricted.
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PMC1402319_01_1742-6413-3-1-3.jpg
F3
CD19, CD20, CD11c bright, CD25 bright, CD103 and were lambda light chain restricted. CD10, CD5 and CD3 were negative (figure 3).