PMC3279321_01
Hb HPLC of patient showing high HbF along with a peak in D window (RT - 4.06 minutes).
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HPLC was advised to look for increased fetal haemoglobin. High Performance Liquid Chromatography (HPLC) was done using BioRad Variant II instrument with beta thalassemia short program. Hb HPLC chromatogram showed a raised HbF (11%) with HbA0 69.2%, HbA2 1.8% and a peak in D-window of 10.2% with a retention time of 4.06 minutes (Figure 1).
Hb HPLC of father (2A) and mother (2B) showing normal chromatogram.
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Both mother and father showed normal Hb HPLC (Figure 2A and 2B).