PMC3770251_01
Light microscopic findings at first renal biopsy. At low magnification, the glomerulus shows MGAs. At higher magnification, bubbling of the GBM is revealed by PAM stain. a Periodic acid Schiff (PAS) stain, x400. b PAM stain, x400. c, d PAM stain, x2,000.
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F1
However, under light microscopy at a higher magnification under periodic acid methenamine silver (PAM) staining, the glomeruli had a bubbling appearance of diffuse glomerular loops (fig. 1).
Electron microscopic findings at the first renal biopsy. Several microspheres are shown in the GBM of a capillary loop (arrowheads).
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F2
Electron microscopy revealed microspheres in the GBM, which were sparse but global (fig. 2).
Microscopic findings at the second renal biopsy. Light microscopic findings show segmental sclerosis with cellular variant. At higher magnification, bubbling of the GBM is revealed by PAM stain. At electron microscopy, microspheres are shown in the GBM (arrowheads), although microspheres are sparser than at the first biopsy. a Light microscopic findings, PAS stain, x400. b, c Light microscopic findings, PAM stain, x2,000. d Electron microscopic findings.
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F3
The microspheres observed in the GBM were sparser than in the first biopsy (fig. 3).