PMC6389543_01
On day 2. The left fifth finger showed redness, swelling, ecchymosis and tense blisters.
gr1.jpg
PMC6389543_01_gr1.jpg
fig0005
The left fifth finger showed redness, swelling, tenderness and tense blisters (Fig. 1).
On day 7. Redness and swelling gradually dissipated in about one week and the fundus was scarlet when blisters were removed.
gr2.jpg
PMC6389543_01_gr2.jpg
fig0010
The redness, swelling and pain of the finger were improved and the skin lesions did not progress to deep necrotic fasciitis (Fig. 2).
Reads mapped to V. vulnificus derived from NGS data. A total of 3114 reads mapped to V. vulnificus in the reference database which contains 2473 bacterial genomes, and got a total coverage of 3.54%.
gr3.jpg
PMC6389543_01_gr3.jpg
fig0015
Three days later, NGS detected the pathogen and confirmed V. vulnificus infection (Fig. 3).
PCR detection of V. vulnificus from tissue sample. Lane M: TAKARA DL2000. Lane 1: PCR product from tissue sample. Lane 2: negative control.
gr4.jpg
PMC6389543_01_gr4.jpg
fig0020
The result of polymerase chain reaction(PCR) was consistent with the findings of NGS (Fig. 4).
On day 14. The left fifth finger was ultimately back to normal.
gr5.jpg
PMC6389543_01_gr5.jpg
fig0025
On the two weeks of hospitalization, the left fifth finger of the patient had improved and the patient was discharged from hospital (Fig. 5).