PMC3235946_01
Frozen tissue cross sections incubated with anti-fast myosin heavy chain (F59). Fibers that were stained for FITC (green) were identified as type II fibers, and negatively stained fibers were identified as type I fibers. The basal lamina of each fiber was stained (red) with an anti-laminin antibody. (a) Type I and type II fibers from the vastus lateralis muscle obtained before exercise training. (b) Type I and type II fibers from the vastus lateralis muscle obtained after 16 weeks of strength training. (c) Quantification of the mean fiber area from the vastus lateralis. These data include both type I and type II fibers. The data were obtained before and after strength training. The data represent mean +- standard deviation of fiber area. (d) Type I and type II fiber areas of the vastus lateralis before and after strength training.
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fig1
Examples of tissue cross sections are shown in Figure 1.
Examples of tissue cross-sections. (a) DAPI-stained nuclei. (b) Apoptotic nuclei as indicated by the TUNEL assay. (c) The basal lamina of fibers was identified by immunocytochemistry. (d) Overlay of DAPI, TUNEL, and lamina staining. The arrows indicate examples of apoptotic nuclei.
CRIM2011-205691.002.jpg
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fig2
Apoptotic nuclei were present (Figure 2), but they made up a small percentage of the total myonuclei before strength training (1.9%).