PMC8490061_01
Gram staining revealing plenty of pus cells with fungal spores (Conidia) but lacking bacteria, when observed under a 100X Olympus CX21 compound microscope.
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fig1
KOH mount revealing the septate fungal hyphae with the characteristic dichotomous branching, when observed under a 40X Olympus CX21 compound microscope.
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fig2
The KOH mount exhibited the septate fungal hyphae with dichotomous branching (a typical feature of Aspergillus spp.) (Figure 2).
Aliquots from the culture sample grown on CHOC, BAP, MAC, and SDA media indicate the growth of Aspergillus spp.
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fig3
The results indicated that there was no bacterial growth; however, fungal growth was observed in all three media plates (Figure 3).
Aspergillus spp. observed as dark brown/black colonies grown on the SDA medium.
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fig4
After 48 hours of incubation in an SDA medium, the fungal growth (Figure 4) was further inoculated into a corn meal agar (CMA) and cultured for 72 hours.
Confirmation of Aspergillus niger with the specific phenotypic characterization of conidiophores, vesicles, metulae, biseriate phialides, and conidia in the LPCB mount, observed under a 100X Olympus CX21 compound microscope.
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fig5
The microscopy results from the LPCB mount revealed the presence of multiple conidia, conidiophores, vesicles, phialides, and metulae resembling Aspergillus spp. (Figure 5).