PMC6005278_01
Nodules (one ulcerated) on the distal extremity and digits.
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fig1
On dermatological examination, 9 ulcerated and nonulcerated skin nodules were observed, ranged from 1 to 7 cm in diameter, and distributed mainly over bony prominences of distal extremities and digits of the front legs (Figure 1), left elbow, and right hock.
Hyperkeratosis, depigmentation, and erosions of footpads.
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fig2
Other skin lesions included footpad hyperkeratosis, crusting, depigmentation, and erosions (Figure 2).
Light microscopy of lactophenol cotton blue slide preparations made of culture colonies revealed round sporangia containing spherical sporangiospores similar to those of P. wickerhamii.
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fig3
P. wickerhamii (Figure 3). P. zopfii cells are oval or cylindrical in shape, producing sporangia of larger diameter (15-25 mum) containing up to 20 sporangiospores.
Nodular-to-diffuse, pyogranulomatous panniculitis with numerous Prototheca-like elements stained vividly purple with PAS stain.
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fig4
Alignment of the sequence obtained in this case to the corresponding fragment of the GenBank number AB183198 sequence. Highlighted letters indicate the differences. The image was edited with Krita 2.8.5 (https://krita.org).
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fig5
Similar sequences were searched in the GenBank with the aid of the Web interface of Blast software which returned 9 of these sequences that belonged to Prototheca wickerhamii strains; the higher similarity applied to GenBank number AB183198 sequence (Figure 5).