PMC4323767_01
Taxonomical classification of 16S rDNA found in reported samples.. A and B: Tree view of 16S rDNA sequences from culture-positive urine (alpha-hemolytic Streptococcus >105 CFU/ml), assigned at different taxonomy levels (family, genus and species) as computed by MEGAN 3.4 for V1V2 (A) and V6 (B) amplicons. Each circle represents a taxon and is labeled by its name and the number of sequenced reads assigned. Taxonomical assignment was done by comparing the sequences to a curated version of the SSUrdp database, then assigning the sequences to the taxon of the best-matched reference sequence (lowest common ancestor). The size of the circles is scaled logarithmically to the number of reads assigned to the taxon.. C and D: Comparison of bacterial communities from culture-positive urine positive for alpha-hemolytic green Streptococcus (red bars) and culture-negative urine (blue bars) from a case subject diagnosed with overactive bladder syndrome. Sequence analysis is based on V1V2 (C) and V6 (D) amplicons. Only major genera with a sequence abundance >=10 % for either V1V2 or V6 analysis are shown. The calculation of sequence abundance for each genus was relative to the total number of sequence assigned to Bacteria in each dataset.
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PMC4323767_01_TOMICROJ-8-148_F1.jpg
F1
In contrast to the clinical reports, the molecular analysis revealed a complex bacterial composition of the urine sample taken (Fig. 1A, 1B). However, the previously dominant genus Atopobium was completely absent this time (Fig. 1C, 1D).