PMC9619092_01
(A) The microscopic evaluation shows an extensively necrotic and hemorrhagic tumor with a distinctive biphasic appearance consisting of cytotrophoblasts, clear to light pink cytoplasm, distinct cell borders, irregular nuclei, and prominent nucleoli. Immunostaining of GATA-3 and SALL4, in close association with and capped by multinucleated syncytiotrophoblasts with abundant deeply eosinophilic cytoplasm, one or more nuclei, dark smudgy chromatin, and immunostaining of inhibin A and beta-hCG (not shown). The neoplasm shows no immunostaining of TTF-1 (not shown).
fonc-12-1001627-g001.jpg
PMC9619092_01_fonc-12-1001627-g001.jpg
f1
Pathologic staging was pT2a pN0 R0 (stage IB, AJCC 8th ed) ( Figure 1 ).
Comparative analysis of polymorphic markers (AmpFLSTR  Identifiler  Plus PCR Amplification Kit) in the tumor sample, patient (blood sample1) and partner (blood sample 2) blood samples. The profile of tumor s and patient s DNA are coincident, with no contribution from the partner`s DNA (in the case of informative markers) (image courtesy of Margarida Reis-Lima, MD, Elsa Garcia, MSc, Natalia Salgueiro, MSc from Synlab laboratories, Porto, Portugal).
fonc-12-1001627-g002.jpg
PMC9619092_01_fonc-12-1001627-g002.jpg
f2
By comparing STR from the tumor and peripheral blood samples from the patient and her husband, we observed a tumor DNA profile consisting exclusively of the patient's genetic pool, with no other donor contribution ( Figure 2 ).
Timeline with relevant data from the episode of care. US - Ultrasound; MTX - methotrexate; GTN - Gestational trophoblastic neoplasia; CCA - choriocarcinoma.
fonc-12-1001627-g003.jpg
PMC9619092_01_fonc-12-1001627-g003.jpg
f3