PMC6441490_01
Distinct Cytoplasmic patterns identified in Hep-2 ANA IIF (Euroimmun)
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F1
The structures recognized by patient serum were distinct cytoplasmic rods and rings (figure 1 and 3). We observed one to two rods and/or rings per cell including some intermediate structures such as a figure ''8'' (Figure 3, B1; B2), elongated rings (Figure 3, A2 ), twisted rings (Figure 3, C2), rods with pin loops (Figure 3). Some rods often align adjacent to the nucleus or perpendicular to the nucleus, and rings may be found in the cytoplasm (Figure 3, A3; B3; C1).
Mitochondrial like cytoplasmic pattern: Panel A: Hep-2 IIF pattern; Panel B: IIF on commercial rat liver, kidney and stomach; Panel C: Line immunoassay ALD profile.
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F2
Cytoplasm of dividing cells was strongly positive (Figure 2, Panel A). IIF on a commercial rat liver, kidney and stomach tissue with the use of polyclonal IgG antibodies confirmed the presence of AMAs, with a characteristic staining pattern: granular diffuse cytoplasmic staining of the Kupffer cells and hepatocytes, of the renal tubules (strongest staining is noted in distal which is mitochondria-rich) and parietal gastric cells (Figure 2, panel B). M2-E3 (BPO) but not against Sp100, PML, gp210, LKM-1, LC-1 and SLA/LP (Figure 2, panel C). We observed one to two rods and/or rings per cell including some intermediate structures such as a figure ''8'' (Figure 3, B1; B2), elongated rings (Figure 3, A2 ), twisted rings (Figure 3, C2), rods with pin loops (Figure 3).
Examples of various intermediate R&R structures in HEp-2 cells stained with patient serum (FICH-green): Curved rod adjacent to the nuclear envelope (A1; C3); elongated ring (A2); elongated, twisted ring (C2); rod with a pin-loop (A3; B3;C1); Figure ''8'' structure (B1;B2)
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F3
The structures recognized by patient serum were distinct cytoplasmic rods and rings (figure 1 and 3). We observed one to two rods and/or rings per cell including some intermediate structures such as a figure ''8'' (Figure 3, B1; B2), elongated rings (Figure 3, A2 ), twisted rings (Figure 3, C2), rods with pin loops (Figure 3). Some rods often align adjacent to the nucleus or perpendicular to the nucleus, and rings may be found in the cytoplasm (Figure 3, A3; B3; C1).