PMC5223285_01
Histological examination of cerebellar sections after staining with hematoxylin and eosin. a: Cerebellum of the CA affected foal 10 x: Almost complete absence of Purkinje cells. The remaining Purkinje cells are shrunken and hyperchromatic. b: Cerebellum of the CA affected foal 40 x.
OpenVetJ-6-259-g001.jpg
PMC5223285_01_OpenVetJ-6-259-g001.jpg
F1
Histopathological studies (Fig 1a, b) of serial transverse cerebellar sections and electron microscopy (JEM 1200EX II, Jeol), revealed morphological characteristics of apoptotic cells with pyknotic nuclei and degenerate mitochondria, cytoplasmic condensation and areas with absence of Purkinje cells, allowing us to confirm the CA diagnosis (Fig 2a, b, c).
Electron microscopy (JEM 1200EX II, Jeol) of a cerebellar section from the CA-affected foal (Camera ES500W Erlangshen C). (a): Morphological characteristics of apoptotic cells (0.5 microm). (b-c): degenerate mitochondria, pale, swollen and vacuolated Purkinje cells (0.2 microm).
OpenVetJ-6-259-g002.jpg
PMC5223285_01_OpenVetJ-6-259-g002.jpg
F2
Histopathological studies (Fig 1a, b) of serial transverse cerebellar sections and electron microscopy (JEM 1200EX II, Jeol), revealed morphological characteristics of apoptotic cells with pyknotic nuclei and degenerate mitochondria, cytoplasmic condensation and areas with absence of Purkinje cells, allowing us to confirm the CA diagnosis (Fig 2a, b, c).