PMC3520642_01
Immunohistochemical study proved to be positive for CD68. Immunophenotyping confirms the histiocytic lineage through an overexpression of the CD68 antigen
rbhh-33-155-g01.jpg
PMC3520642_01_rbhh-33-155-g01.jpg
f01
Eventually a sample was sent to Washington, USA where morphological and immunohistochemical alterations compatible with histiocytic sarcoma were identified: large cells with irregular-shaped nuclei and nucleoli, showing some binucleate forms and eosinophilic cytoplasm; positive immunophenotype for the CD68, CD163 and CD4 antigens which establish histiocytic proliferation and negative for CD1a and Langerin, which exclude the differential diagnosis of giant cell lymphoma and dendritic cell sarcoma. (Figures 1 & 2).
Immunohistochemical study showing atypical histiocytic proliferation, diffuse proliferation of large cells with irregular nuclei, conspicuous nucleoli and abundant cytoplasm
rbhh-33-155-g02.jpg
PMC3520642_01_rbhh-33-155-g02.jpg
f02
Eventually a sample was sent to Washington, USA where morphological and immunohistochemical alterations compatible with histiocytic sarcoma were identified: large cells with irregular-shaped nuclei and nucleoli, showing some binucleate forms and eosinophilic cytoplasm; positive immunophenotype for the CD68, CD163 and CD4 antigens which establish histiocytic proliferation and negative for CD1a and Langerin, which exclude the differential diagnosis of giant cell lymphoma and dendritic cell sarcoma. (Figures 1 & 2).