PMC9997460_01
Mean arterial pressure response to methylene blue. Aggregate minute-by-minute mean arterial pressure (MAP) collected for 30 min prior to methylene blue (MB) administration (time = 0.0 h) to 3 h (3.0 h) after MB. MAP values for all 13 patients were averaged at each time point. Simple linear regression analysis was used to analyze the slope and elevation between the best-fit lines pre- and post- MB. ***= P < 0.001
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F1
MAP data were plotted pre- and post-MB administration and simple linear regression was used to evaluate the slope and elevation of best-fit lines for significant difference (GraphPad Prism version 8.4, GraphPad Software, La Jolla, CA). MB was associated with a significant (P < 0.001) increase in MAP when patients were analyzed in aggregate [Figure 1].
(a) Patient-specific mean arterial pressure and norepinephrine equivalent response to methylene blue treatment. Individual patient mean arterial pressure (MAP) readings with corresponding vasopressor requirement expressed as norepinephrine equivalents (NEE) to show hemodynamic response to methylene blue (MB). Time zero indicates when MB was administered. Time points were recorded for each patient every 1 or 5 min. Final time points reflect end of procedure following administration of MB. (b) Figure does not include medication boluses in this time frame. MAP values prior to MB are depicted in red, MAP values following MB are depicted in orange. Simple linear regression analysis was used to analyze the slope and elevation between the best-fit lines pre- and post-MB. *=P < 0.10, **= P < 0.05, ***= P < 0.001
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F2
For 11/13 patients, there was a reduction or no change in pressor requirement as measured in NEE [Table 1, Figure 2] following MB administration, but there was a significant increase in MAP (P-value < 0.05 by simple linear regression analysis) in all 13 patients individually [Figure 2].