PMC5381803_01
PCR comparing wild-type VZV and vVZV. Lane 5 shows the patient presented in this report.. DNA markers: 622-67 bp.. Lane 1: Uncut wild-type VZV DNA control: 350- and 222-bp bands. 1a: BgL I digestion: The 222-bp band is uncut.. 1b: Pst I digestion: The 350-bp band is digested, resulting in fragments of 250 and 100 bp.. Lane 2: Uncut vVZV DNA control: 350- and 222-bp bands. 2a: BgL I digestion: The 222-bp band digested, resulting in fragments of 135 and 87 bp.. 2b: Pst I digestion: The 350-bp band is uncut.. Lane 3: Uncut amplified DNA from a clinical sample: 350- and 222-bp bands. 3a: BgL I digestion: The 222-bp band is uncut.. 3b: Pst I digestion: The 350-bp band is digested, resulting in fragments of 250 and 100 bp. Interpretation: wVZV. Lane 4: Uncut amplified DNA from a clinical sample: 350- and 222-bp bands. 4a: BgL I digestion: The 222-bp band is uncut.. 4b: Pst I digestion: The 350-bp band is digested, resulting in fragments of 250 and 100 bp. Interpretation: wVZV. Lane 5: Uncut amplified DNA from the patient reported here: 350- and 222-bp bands. 5a: BgL I digestion: The 222-bp band digested, resulting in fragments of 135 and 87 bp.. 5b: Pst I digestion: The 350-bp band is uncut. Interpretation: vVZV. Lane 6: Uncut amplified DNA from a clinical sample: 350- and 222-bp bands. 6a: BgL I digestion: The 222-bp band digested, resulting in fragments of 135 and 87 bp. 6b: Pst I digestion: The 350-bp band digested, resulting in fragments of 250 and 100 bp. Interpretation: vVZV.
nihms854481f1.jpg
PMC5381803_01_nihms854481f1.jpg
F1
A fluid specimen from another lesion was analyzed by polymerase chain reaction (PCR), which revealed vVZV (Fig 1).