PMC6019596_01
Mucocutaneous lesions on the face
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F1
Ulcerative lesion on the right hand
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F2
The PCR-RFLP on specimens of lesions
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F3
TTT CCG ATG) and L5.8s: 5-TGA TAC CAC TTA TCG CAC TT was designed for LTS1-PCR and cutting with and HaeIII enzyme. Positive controls containing DNA of L. major, L. tropica and a negative control containing distilled water were included. The PCR-RFLP on specimens of lesions proved L. major as the pathogenic agent (Fig. 3).
Significant treatment of lesion by meglumine antimoniate
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F4
Significant treatment of lesion by meglumine antimoniate
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F5