PMC10348747_01
beta-globin gene sequencing showing the point mutation at c.202G>A before HSCT (A) and a normal sequence after HSCT (B). The arrow shows the position of the mutation.
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DNA sequencing of the globin genes were subsequently performed and identified a heterozygous mutation in Hbbeta codon 67, variant c.202G>A Val-Met ( Figure 1A , GenBank accession No. DNA sequencing of globin chains. Deferasirox was administered at 4 years of age because of iron overload, with a serum ferritin level >2000 ng/mL ( Supplementary Figure 1 ). Furthermore, the former mutation of Hbbeta was not detected using DNA resequencing ( Figure 1B , GenBank accession No.
Overview of the haplo-HSCT protocol.
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As the patient did not have matched siblings or unrelated donors, he underwent haplo-HSCT with a myeloablative conditioning regimen ( Figure 2 ).