PMC9799051_01
Muscle biopsy of the quadriceps femoris. (A) Oil Red O (ORO) stain showing lipid droplets deposited in some muscle fibers. (B,C) Lipid droplet is almost invisible in succinate dehydrogenase (SDH) and nicotinamide adenine dinucleotide (NADH) stains. (D) Hematoxylin and eosin (H and E) stain demonstrating abundant vacuoles appearing in most fibers. (E,F) Considerable lipid droplets are observed by transmission electron microscopy.
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F1
Muscle biopsies of the biceps brachii and quadriceps femoris revealed increased deposition of lipid droplets in muscle fibers stained with Oil Red O (ORO) (Figure 1A), which indicated lipid storage myopathy (Figure 1). However, succinate dehydrogenase (SDH) (Figure 1B) and nicotinamide adenine dinucleotide-tetrazolium reductase (NADH-TR) (Figure 1C) were negative for fat vacuole deposition. E) (Figure 1D) stain showed a little inflammatory cell infiltration, and abundant small vacuoles were observed in a large number of muscle fibers. Electron microscopy demonstrated that lipid droplets increased in subsarcomembrane and of myofibrils.
PMC9799051_02