PMC4272950_01
Bone marrow biopsy after 6 cycles of rituximab, cyclophosphamide, doxorubicin, vincristine and prednisolone (R-CHOP) combination chemotherapy. (A, B) Neoplastic B lymphocytes infiltrating in an interstitial pattern was immunohistochemically analyzed by anti-PAX5 antibody (A, x100; B, x1,000). (C, D) Neoplastic T lymphocytes infiltrating with a peritrabecular or interstitial pattern was immunohistochemically analyzed by anti-CD3 antibody (C, x100; D, x1,000).
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F1
PAX5-positive lymphocytes with a marked interstitial infiltration pattern (Fig. 1A, B), and the cells were also CD79a-positive. Peritrabecular and interstitial infiltration of small to medium sized CD3-positive lymphocytes was also prominent (Fig. 1C, D).
T cell receptor gamma locus (TRG) rearrangement analyzed by using BIOMED-2 multiplex PCR. Horizontal axis represents amplicon size (base pairs) and vertical axis represents fluorescence intensity. (A) A monoclonal TRG rearrangement was detected at 170 bp in the liver sample. (B) Both a larger peak (160 bp) and a smaller peak (170 bp) were observed in the bone marrow aspirate sample by GeneScan analysis of the PCR results.
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F2
A 170-bp monoclonal TRG rearrangement was detected in the liver sample (Fig. 2A), while TRG in the BM had a larger 160-bp peak with a smaller 170-bp peak (Fig. 2B).