PMC7388409_01
PMC7388409_02
PMC7388409_03
PMC7388409_04
PMC7388409_05
PMC7388409_06
CENP-F like, dense fine speckled nuclear and dense fine speckled cytoplasmic.
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PMC7388409_06_gr1.jpg
fig1
Anti-Yo antibodies, IIF on HEp-2 cells showed ANA patterns classified by ICAP (International consensus on ANA patterns) as following: CENP-F like/AC-14, dense fine speckled nuclear/AC-2 and dense fine speckled cytoplasmic/AC-19 (Fig. 1). Almost a month later with the serum after mastectomy and prior to PCD treatment with the peak in anti-Yo levels (39++), the ANA results had a finer speckled nuclear pattern (AC-4) and occasional HEp-2 cells with PCNA like pattern (AC-13) (Fig. 2).
Finer speckled nuclear pattern and occasional HEp-2 cells with PCNA like pattern.
gr2.jpg
PMC7388409_06_gr2.jpg
fig2
Anti-Yo antibodies, IIF on HEp-2 cells showed ANA patterns classified by ICAP (International consensus on ANA patterns) as following: CENP-F like/AC-14, dense fine speckled nuclear/AC-2 and dense fine speckled cytoplasmic/AC-19 (Fig. 1). Almost a month later with the serum after mastectomy and prior to PCD treatment with the peak in anti-Yo levels (39++), the ANA results had a finer speckled nuclear pattern (AC-4) and occasional HEp-2 cells with PCNA like pattern (AC-13) (Fig. 2).
Same patterns, with a note that the speckled nuclear patterns had distinctive nucleolar staining in terms of SSB or Scl antigen association.
gr3.jpg
PMC7388409_06_gr3.jpg
fig3
The third serum sample was after initiating treatment for PCD, almost two months later, the anti-Yo levels dropped to the lowest point of + (8) on immunoblot, the ANA results were with the same patterns, with a note that the speckled nuclear patterns had distinctive nucleolar staining in terms of SSB or Scl antigen association (Fig. 3).
Cytoplasmic patterns are more fluorescent than nuclear, the nucleoli aren't that accentuated anymore and the CENP-F-like pattern isnot as fluorescent as on the first ANA testing.
gr4.jpg
PMC7388409_06_gr4.jpg
fig4
The final ANA results were a month later in May, with a note that cytoplasmic patterns were more fluorescent than nuclear, the nucleoli weren't that accentuated anymore and the CENP-F-like pattern was not as fluorescent as on the first ANA testing (Fig. 4).