PMC4155769_01
DNA Sequencing of BCR-ABL e1a3 atypical translocation. We observed an amplification band of approximately 100 bp (e1a3). Besides this band we included the b2a2 (200 bp) and b3a2 (300 bp) BCR-ABL control bands. In order to confirm the presence of a BCR-ABL transcript this band was extracted from the agarose gel, purified and then analyzed by DNA sequencing.
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F1
In order to confirm the presence of a BCR-ABL transcript this band was extracted from the agarose gel, purified and then analyzed by DNA sequencing. cDNA sequence confirmed the presence of the e1a3 BCR-ABL transcript (Figure 1).
Immnunophenotype of ALL with atypical e1a3 translocation. Presence of a 70% of blasts with lymphoblastic phenotype B: CD19+, CD10+, CD34+, DR+, CD20 - CD22 -, cytoplasmic IgM negative and positive alpha CD79.
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F2
The rest of myeloid and T-cell studied markers expression were negative (Figure 2).