PMC5337370_01
Low power H&E slide. The lobular architecture with cribriform pattern can be identified. There is no connection to the epidermis.
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fig1
It displayed a lobular architecture with solid and cribriforming areas (Figure 1).
High power H&E slide. The nuclei are round to oval with conspicuous nucleoli and a finely stippled chromatin pattern.
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fig2
The nuclei were medium-sized, monomorphic, and round to oval with 1 to 2 conspicuous nucleoli, the chromatin was finely stippled and resembled the "salt and pepper" pattern seen in neuroendocrine tumors, and there was a moderate amount of eosinophilic cytoplasm (Figure 2).
High power H&E slide. Both intracytoplasmic and extracellular mucin are present.
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fig3
Intracytoplasmic mucin could be identified, as it displayed a light blue hue on H&E stained slides, and extracellular mucin was also present throughout the tumor (Figure 3).
Positive synaptophysin staining.
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fig4
There was strong immunohistochemical staining for synaptophysin (Figure 4), chromogranin, NSE, and CD57 and focal positive staining for CD56.
Positive estrogen receptor staining (90-100%).
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fig5
Estrogen and progesterone nuclear stains were positive in 90-100% of the tumor cells (Figure 5).
Smooth muscle myosin heavy chain (SMMHC) highlights the myoepithelial cells around the lobules.
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fig6
Smooth muscle myosin heavy chain (SMMHC) stained focal myoepithelial cells around the larger lobules (Figure 6).