PMC5584510_01
Organization of nod genes in Frankia NRRL B-16219 and Candidatus Frankia datiscae Dg1 genomes. Sizes, localization and orientation of the genes are displayed proportionally. These genes are not detectable in any other Frankia genome except Candidatus Frankia Dg2
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PMC5584510_01_40793_2017_261_Fig3_HTML.jpg
Fig3
Table S1) organized into two regions (Fig. 3).
Maximum likelihood phylogeny based on amino acids of nodA (a), nodB (b), nodC (c) and nodH (d). GC-content is provided for nod genes and for genomes (in parenthesis). Bootstrap and probability values larger than 50% are only shown
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PMC5584510_01_40793_2017_261_Fig4_HTML.jpg
Fig4
Further phylogenetic analysis (Fig. 4) showed that the 10.1601/nm.7635 Nod proteins were positioned at the root of both the alpha- and beta-rhizobial NodABC proteins as previously reported.
Neighbor-Joining phylogenetic tree based on glnA gene sequences. Bootstrap and probability values larger than 50% are only shown. Marked in bold are Frankia strains or microsymbionts with nod genes as present in their genomes or detected by PCR-sequencing analysis
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PMC5584510_01_40793_2017_261_Fig5_HTML.jpg
Fig5
Regardless of their affiliation to cluster 2 or to cluster 3 (Fig. 5), all of the analyzed C. americanus microsymbionts contained the nodAB genes.