PMC9848784_01
Swelling on the forehead is obvious along with periocular hypotrichosis.
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fig1
Periocular hypotrichosis, edema, and slight hyperpigmentation were additional skin lesions (Figure 1).
Edema of the conjunctiva and third eyelid of the right eye.
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fig2
Apart from skin lesions, edema of the conjunctiva and third eyelid of the right eye were also observed (Figure 2).
Photomicrography of the swelling of the forehead of the cat with leishmaniosis showing granulomatous inflammation with high number of Leishmania amastigotes, both intracellularly (arrowhead) and extracellularly (arrow). In some amastigotes, the basophilic rod-shaped kinetoplast is clearly visible (asterisk) (Diff-Quick stain, x400).
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fig3
Photomicrography showing granulomatous panniculitis with high number of Leishmania amastigotes (Hematoxylin and Eosin stain, x400).
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fig4
PCR amplified products (lanes 1 and 6: 100 bp DNA ladder; lane 2: FNAB sample; lane 3: positive control for L. infantum; lane 4: ATCC NR-50127 positive control for L. tropica; lane 5: negative control).
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fig5
The amplified PCR product corresponded to the expected 300-350 bp for Leishmania spp. (Figure 5).
Four months after the start of therapy, the cat looks normal.
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fig6
A second clinical evaluation performed after 4 months revealed no abnormalities (Figure 6).