PMC3647713_01
Major clinical events occurred in the patient after an ABO incompatible (donor A Rh+; recipient 0 Rh+) HLA matched sibling for beta-thalassemia major.
mjhid-5-1-e2013029f1.jpg
PMC3647713_01_mjhid-5-1-e2013029f1.jpg
f1-mjhid-5-1-e2013029
Subsequent detections showed a progressive reduction of donor chimerism (60% on day + 80; 50% on day +100). (Figure 1) Two months and a half later (+150) reduced mixed chimerism was confirmed (50% of donor cells) and due to this, five increasing dose infusions (from 1x104/kg to 2x105/kg) of donor lymphocytes (DLI) were performed from September 2008 to February 2009, in order to further increase donor chimerism. In fact, chimerism studies on both peripheral blood and bone marrow showed a complete recipient chimerism, with the absence of donor-derived hematopoietic cells (Figure 1 and Figure 2).
White blood cells count of the patient after transplants.
mjhid-5-1-e2013029f2.jpg
PMC3647713_01_mjhid-5-1-e2013029f2.jpg
f2-mjhid-5-1-e2013029
In fact, chimerism studies on both peripheral blood and bone marrow showed a complete recipient chimerism, with the absence of donor-derived hematopoietic cells (Figure 1 and Figure 2).