PMC5283968_01
A: Chest X-ray shows a nodule in the right lower lung (arrow). B: Nine months after performing a biopsy, the nodule has become vague (arrow).
1349-7235-55-3655-g001.jpg
PMC5283968_01_1349-7235-55-3655-g001.jpg
g001
A chest X-ray and chest CT showed a nodule measuring 12-mm diameter localized in the right lower lobe (Figure 1A, 2A).
A: Chest computed tomography showed a solitary nodule (12 mm in diameter) in the right lower lobe just before undergoing biopsy (arrow). B: Three months after the biopsy, the diameter of the nodule has slightly increased (arrow). C: Fifteen months after the biopsy, the nodule has disappeared (arrow).
1349-7235-55-3655-g002.jpg
PMC5283968_01_1349-7235-55-3655-g002.jpg
g002
A chest X-ray and chest CT showed a nodule measuring 12-mm diameter localized in the right lower lobe (Figure 1A, 2A). The solitary lung nodule increased slightly on the CT image at three months after the biopsy (Fig. 2B), but subsequently became unclear on a chest X-ray nine months after the biopsy (Fig. 1B), and then it completely disappeared on the CT image 15 months after the biopsy (Fig. 2C).
A: Histological examinations showed an accumulation of small lymphoid cells [Hematoxylin and Eosin (H&E) staining, x40]. B: Lymphoid cells consist of atypical small lymphocytes (H&E staining, x200). C: Cells in the lymphatic follicle include clusters of differentiation (CD) 20 positive (CD20 stain, x40). D: Infiltration of lymphoid cells into the epithelium, defined as lympho-epithelial lesion (LEL) (Cytokeratin stain, x200).
1349-7235-55-3655-g003.jpg
PMC5283968_01_1349-7235-55-3655-g003.jpg
g003
Histological examination with Hematoxylin and Eosin (H&E) staining showed the proliferation of small lymphocytes with little atypia (Fig. 3A and B). Immunohistochemical staining proved that most of the cells in the lymphatic follicles were clusters of differentiation (CD) 20 positive (Fig. 3C). Immunostaining of cytokeratin confirmed the presence of a lympho-epithelial lesion (LEL), which indicated the infiltration of lymphocytes into the epithelium of the gland duct (Fig. 3D).
GeneScan results of polymerase chain reaction products of the biopsied sample showing the gene rearrangement of the immunoglobulin heavy chain DH1-6-JH. The x-axes represent the molecular size (base pairs), and the y-axes represent the fluorescence intensity. A: Positive control of the peak of DH1-6-JH. B: DH1-6-JH gene products of the tumor (arrow) showing a higher peak than the positive control (A). C: Polyclonal control.
1349-7235-55-3655-g004.jpg
PMC5283968_01_1349-7235-55-3655-g004.jpg
g004