PMC8576158_01
A) The tumor mass filled from anterior to posterior chambers. 1: Sclera 2: Tumor mass was located at the caudal aspect of cornea 3: Interspace between sclera and tumor mass 4: Tumor mass is filling the vitreous body 5: The sclera is wrinkled at the posterior end of the globe. (Atrophied ocular lens is removed); B) Subgross photomicrograph of ovine globe tumor, combined of pigmented and nonpigmented parts of iridociliary adenoma (asterisks). C: Cornea (Alcian blue; Scale bar = 3.00 mm)
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F1
The entire globe had been filled with dark fleshy solid tissue (3.00 cm x 2.30 cm), the tumor mass was extended from both the posterior aspect of the cornea toward the lens and from the posterior aspect of the lens to the caudal end of the eyeball (Fig. 1).
A) Round to ovoid pigmented cells (arrows) in contrast to spindle-shaped cells (right). The epithelioid-like cells are present among the others (arrowheads), (H&E; Scale bar = 30.00 microm). B) Large numbers of neoplastic cells scattered in the relatively loose connective tissue stroma consisted of numerous closely packed thin-walled blood vessels (Alcian blue-PAS; Scale bar = 400 microm). C) The cytoplasms of epithelial tumor cells are immunopositive for vimentin, (SAB immunolabeling with Mayer's Hematoxylin counterstain; Scale bar = 200 microm). D) The solid mass of epithelial tumor cells is non-immunoreactive for S-100 protein (Scale bar = 200 microm)
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F2
The first group consisted of separated cells with spindle-shaped, round to polygonal cells with high cytoplasm volume, and the presence of black granules. The second group consisted of cells in the glandular form consisting of rows of cylindrical cells with a pigmented cytoplasm surrounded by collagen-hard connective tissue (Fig. 2A). However, the result for Alcian blue staining was negative, as well (Fig. 2B). The immunohistochemistry result was positive for vimentin marker (Fig. 2C), but no immuno-reactivity with S-100 (Fig. 2D) and cytokeratin was appeared.