PMC10317141_01
A) Burkitt lymphoma and the "starry-sky" pattern from the extranodal masses section (mesentery region); B) Interphase FISH analysis of MYCC gene. The MYCC (8q24) FISH probe is optimized to detect rearrangements of the MYCC gene region at 8q24 (3 spots in green) in the sample of the patient with Burkitt lymphoma; C) Interphase FISH analysis of the AURKA gene demonstrating elevated DNA copy number in the sample of the patient with Burkitt lymphoma (additional spots in red, control in green); D) Interphase FISH analysis of the AURKB gene demonstrating elevated DNA copy number in the sample of the patient with Burkitt lymphoma (additional spots in red, control in green) (Kreatech FISH Probes, Amsterdam, The Netherlands). Amplifications of AURKA and AURKB genes were observed in all analyzed cells
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A biopsy in the mesentery region revealed the presence of pleomorphic lymphocytes with evident nucleoli and macrophages with clear cytoplasm and a "starry sky" appearance (Figure 1A). MYCC (8q24), AURKA: ON AURKA (20q13)/20q11, and AURKB: AURKB (17/p13)/SE17 (Kreatech Fish Probes, Amsterdam, The Netherlands). MYCC rearrangement was easily identified via iFISH using a break-apart probe or split assay using a green probe that flanked the MYCC locus at 8q24 (Figure 1B), and BL was diagnosed. In addition to MYCC rearrangement, we performed iFISH for aurora kinase genes, and amplification of AURKA and AURKB was observed (Figure 1C and D).