PMC4263470_01
Loss of 4q21.23 in DTCs and tumor tissue of a NSCLC patient.. Bone-marrow cells of the patient were immunocytochemically stained against cytokeratin using the APAAP method. A positive DTC (red) and a negative leukocyte (brown) are shown. B: Bone-marrow cells of the patient were stained fluorescently against cytokeratin (red signal) followed by FISH analysis in C) with RP11-1053C2 probe and Cen3 probe (RP11-1053C2 probe: 1 green signal; Cen3 probe: 3 spectrum orange signals; nuclear staining in DAPI). Cen7 probe (Cen7 probe: spectrum aqua displayed in the pseudo-color magenta) was in addition used for FISH analysis on D) primary tumor (2-5 orange signals/cell, 2-4 magenta signals/cell and 0-2 green signals/cell) and E) tumor relapse FFPE material (3-6 orange, 4-5 magenta and 1-3 green signals/cell).
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pone-0113315-g003
FISH analysis was also performed on paraffin-embedded primary tumor and lung relapse material from the same patient (fig. 3).