PMC5949180_01
Erythematous, denture-related candidiasis in a 14-year-old boy (photography taken in an intraoral mirror).
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To illustrate this method, we present the case of a 14-year-old boy suffering from a denture-related erythematous stomatitis (Figure 1).
Step-by-step procedure for sampling.
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When saliva covers the mirror, it is removed from the mouth and laid on the slide (Figure 2(a)). The sample was subsequently collected with a sterile probe directly placed into the patient's saliva (Figure 2(c)), and a cover slip was mounted. An important point to note is that the sulcus area is the optimal site to collect the sample using this method (Figure 2(b)). The sample was then analyzed under a phase contrast optical microscope (Figure 2(d)).
Candida hyphae (x1000 in patient's saliva).
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In pathological conditions, several hyphae are visible on each screen, mixed with oral bacteria and cells (Figure 3).
(a) Clinical improvement at the 2 years follow-up. (b) Normalization of oral flora after treatment (x1000 in patient's saliva).
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At the two years follow-up, the same procedure showed a normalization of oral flora, with absence of hyphae (Figure 4(b)), linked to a clinical improvement (Figure 4(a)).