PMC10484312_01
Materials and methods: (a) Clinical presentation of three cases at Day 0 (day of surgery), Day 8 (suture removal) and Day 30 (follow-up) assessing the wound healing. (b) 

Staphylococcus arlettae

 solation in pure form on blood agar and BHIA (brain heart infusion agar) media, and bacterial morphology by SEM. (c) Environmental sampling on NAM (nutrient agar medium) and blood agar from dental chair - showing beta hemolytic organism growth and dental water unit lines - showing no microbial growth, microbiology laboratory workstation (BLST) (biosafety cabinet type-IIA) - showing growth other than 

S. arlettae

 and sterile suture on incubation - showing no microbial growth).
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F1
Wound healing was assessed at the time of suture removal and 1 month post-operatively, using the Early Wound Healing Score (Fig. 1a). Sterile sutures from the same batch of manufacturing as those used for the surgeries were inoculated in 5 ml of nutrient broth and observed for growth after incubation at 37  C for 24 h (Fig. 1b, c).
Scanning Electron Microscopy (SEM) images of suture samples with 

S. arlettae

 biofilm formation: (a) Resorbable chlorhexidine coated polyglycolic-lactic acid (PGLA) suture under 150x and 10000x magnification showing biofilm formation. (b) Resorbable non coated PGLA suture under 150x and 10000x magnification showing biofilm formation. (c) Control (sterile suture) under 150x and 10000x magnification. (d) SEM image of 

S. arlettae

 colonies at 10000x magnification.
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F2
Next, sutures were sputter coated with a layer of gold and observed under scanning electron microscope (Aprio, Thermo Fisher Scientific, USA) (Fig. 2).