PMC5220414_01
(a) Histologic section of lymph node showed effaced architecture by infiltration of bipopulation of blastoid cells (H&E stain x100). (b) Positive immunostaining for CD3 in one population of blastoid cells (counterstain with hematoxylin x400). (c) Positive immunostaining for tdt in the same population of blastoid cells (counterstain with hematoxylin x400). (d) Positive immunostaining for MPO in another population of blastoid cells (counterstain with hematoxylin x400).
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fig1
In immunohistochemistry one population was immunoreactive for MPO and CD68 and negative for CD10, CD3, CD20, CD56, CD34, CD138, CD117, tdt, CD99, and S100, and the other showed expression of CD3, CD99, and tdt but not MPO, CD10, CD20, CD56, CD34, CD138, and CD117 (Figures 1(a)-1(d)).
Karyotype analysis of the bone marrow culture revealing t(1;5)(q23;q33).
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fig2
Cytogenetic study on metaphase cells by Q-banding method depicted an interesting finding which was t(1;5)(q23;q33) (Figure 2).