PMC7783198_01
Patient treatment timeline. RRMS, relapsing-remitting multiple sclerosis; DMF, dimethyl fumarate; SPMS, secondary progressive multiple sclerosis; EDSS, Expanded Disability Status Scale.
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F1
As it is summarized in Figure 1, after the approval of the ethical committee (RIF.CE: 4520) a 39-year-old with disease onset in 2005 with visual acuity disturbance and diagnosis of RRMS on July 2008, regularly followed in MS Center S.
Adiponectin serum levels and Serum Acrp30 distribution in healthy controls (n = 10), MS patients (n = 10), and in the MS case undergone a training program at baseline (T0), post physical activity intervention (T1), and at the follow-up (T2): (A) Elisa-test of Adiponectin serum level (mug/ml). The values are reported as mean of the absorbance +- SD; (B) One representative image of western blot oligomeric distribution [HMW (>=250 kDa), MMW (180 kDa), and LMW (70 kDa)]; (C) Graphical representation of pixel quantization of HMW, MMW, and LMW oligomers. For other details see section "outcomes."
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F2
As shown in the Elisa-test (see Figure 2A), we analyzed total serum adiponectin in healthy controls, MS patients, and baseline (T0), post physical activity intervention (T1) and at follow-up (T2) in the MS case patient, in which, we found a decrease of serum adiponectin levels [9.05 mug/ml (T0) vs. 7.78 mug/ml (T1)] before and after physical activity intervention. Furthermore, as shown in the Western blots (Figures 2B,C), we analyzed the distribution of serum Acrp30 in healthy controls, MS patients, and baseline (T0), post physical activity intervention (T1) and at follow-up (T2) in the MS case patient. Three bands corresponding to HMW (>=250 kDa), MMW (180 kDa), and LMW (70 kDa) oligomers were evident in MS patient (Figure 2B). In particular, a more evident decrease in HMW oligomers, the most biologically active oligomers, was found (Figure 2C). The adiponectin oligomerization state at the follow-up (T2), do not substantially change for all oligomers (Figure 2).