PMC6759869_01
Analysis of a CSF-470 VAC-SITE tissue. (A-C) Dense clusters of CD11c+ cells were observed at the VAC-SITE, most of them also expressing PD-L1; (D) Abundant CD68+ macrophages were also present in the area. (E-G) CD8+ lymphocytes were mainly PD1- and many of them were proliferating (Ki67+). (H,I) Numerous LMGC were observed by HE staining (arrows). (J,K) The VAC-SITE was surrounded by numerous lymphatic and blood vessels (arrows). Original magnifications = (A,D-G,J,K): 20X; (B,C,H): 100X; (I): 1000X. Scale bars: (A,D-G,J,K) =200 mum; (B,C,H) = 100 mum; (I) = 20 mum.
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F1
Its main characteristics were a 2.3 mm Breslow thickness, an intermediate proliferative index (23% Ki-67+), and non-brisk lymphoid infiltration; no evidence of ulceration, regression, satellitosis, or vascular invasion were found (Supplementary Figure 1).
Analysis of C-MTS tissue. In the area of tumor regression (upper part of the biopsy), CD8+
(A) and PD-1+
(B) T lymphocytes were mainly present, while FOXP3+ Treg were scarce (C); these lymphocytes were proliferating as determined by Ki-67+ staining (D). Also, CD68+ macrophages (E) and CD11c Ag-presenting cells (F) were mainly concentrated in this area. In the lower part of the biopsy, MART-1+
(G) PD-L1-
(H) HLA class I +
(I) viable tumor cells were observed. Original magnification = 20X. Scale bars = 200 mum.
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PMC6759869_01_fimmu-10-02213-g0002.jpg
F2
Time course of treatment, as well as the surgical events and blood extractions, are indicated (Supplementary Figure 2).