PMC5209591_01
Gross image of the nodule (black arrow) during the surgical procedure. The white arrow points at the serosa of the stomach, from which the nodule was attached.
CRIPA2016-1537240.001.jpg
PMC5209591_01_CRIPA2016-1537240.001.jpg
fig1
A minimally adherent 2 cm lesion located at the gastric fundus near the gastroesophageal junction and the left crus of the diaphragm was completely removed and sent for pathology examination (see Figure 1).
((a) and (b)) Duplication cyst at low power. The arrows point at the epithelial lining of the cyst, H&E. (c) Pseudostratified ciliated epithelial lining of the duplication cyst at high power. The arrow indicates the presence of cilia, H&E. ((d) and (e)) GIST nodule at low and high power. The tumor cells have spindle shape and mild cytologic atypia, H&E. ((f) and (g)) Immunohistochemistry stains show that the tumor cells are strongly positive for both CD117 (f) and CD34 (g). ((h) and (i)) Leiomyoma at low and high power. The tumor cells have elongated nuclei and abundant cytoplasm, with no cytologic atypia, H&E. ((j) and (k)) Immunohistochemistry stains show that the tumor cells are negative for CD117 (j), while they are strongly positive for SMA (k).
CRIPA2016-1537240.002.jpg
PMC5209591_01_CRIPA2016-1537240.002.jpg
fig2
As illustrated in Figure 2, the tumor cells were labelled strongly and diffusely for CD117 (polyclonal rabbit anti-human CD117, Dako, California, USA) and CD34 (monoclonal mouse anti-human CD34 class II clone QBEnd-10, Dako, California, USA) but not for actin (monoclonal mouse anti-human muscle actin clone HHF35, Dako, California, USA), which was in agreement with a low-grade gastrointestinal stromal tumor (GIST).