PMC4020909_01
Histological and radiological diagnosis.. Notes: (A) Histology, liver biopsy. (B) Primary tumor.
ott-7-697Fig1.jpg
PMC4020909_01_ott-7-697Fig1.jpg
f1-ott-7-697
AE1-AE3 and cytokeratins 5/6, transformation-related protein 63, and tyrosine-protein kinase Kit, but not for thyroid transcription factor, Wilms tumor 1, cluster of differentiation 1a, terminal deoxynucleotidyltransferase-positive cells, CD5, chromogranin A, or synaptophysin. A diagnosis was made of poorly differentiated nonkeratinizing squamous cell carcinoma with a prevalent low-grade spindle cell pattern consistent with metastatic thymic carcinoma (Figure 1A and B).
Liver and lung respose to sorafenib.. Notes: (A) and (C) Liver and lung metastasis pre-sorafenib. (B) and (D) Liver and lung metastasis post-sorafenib.
ott-7-697Fig2.jpg
PMC4020909_01_ott-7-697Fig2.jpg
f2-ott-7-697
Tumors (RECIST) criteria, of a 30% reduction of the sum of the maximal diameters of lesions in all sites of disease. In detail, the two pulmonary lesions showed a complete response, while in the other sites of disease, along with the dimensional reduction, a significant reduction of CT scan attenuation (Hounsfield) units was seen. This was probably related to intralesional devascularization with associated necrosis in response to the targeted therapy (Figure 2A-D).
Mutation p.K642E (cod A/Gaa).. Abbreviations: Ref, references sequences; SUM, comparison of references sequences and unknown sample.
ott-7-697Fig3.jpg
PMC4020909_01_ott-7-697Fig3.jpg
f3-ott-7-697
A tumor specimen taken by liver biopsy at the time of diagnosis was then subjected to molecular analysis with polymerase chain reaction-based direct sequencing, and a missense mutation in v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (c-KIT) exon 13 (K642E) was found (Figure 3).