PMC5576958_01
The H&E staining tumor tissue and immunohistochemistry staining tumor tissues for TTF-1, E-cadherin and Vimentin phenotypes. The primary tumor tissues(A,C,E,G). The primary tumor showed moderate differentiated adenocarcinoma(A). The TTF-1 and E-cadherin were positive in the differentiated cancerous tissue but attenuated in the tumor cell clusters(which is indicated by the red arrow in the figure) in the invasive margin of the cancer(C,E). Vemintin were positive in the tumor cell clusters(indicated by the arrow) in the invasive margin of the cancer (G). The expectoration of recurred tumor tissue block(B,D,F,H). The recurred tumor tissue showed, TTF-1,E-cadherin and Vimentin stained sections. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Microscopically, the primary tumor tissues exhibited moderate differentiated glandular and papillary patterns, the micropapillary and tumor cell clusters were scattered in the peripheral invasive margin (Fig. 1 A). TTF-1 was positive in the differentiated glandular cancerous tissue but weak/negative in the tumor cell clusters in the invasive margin (Fig. 1 C, Table 1). E-cadherin were positive in the differentiated cancerous tissue but attenuated in the tumor cell clusters in the invasive margin too (Fig. 1 E, Table 1), while Vimentin was negative in the differentiated cancerous tissue but positive in the tumor cell clusters in the infiltrating margin of the cancer [Fig. 1G, Table 1]. TTF-1, strong Vimentine but attenuated E-cadherin staining were demonstrated (Fig. 1H,F). These results suggested that the recurred lesions had undergone an EMT phenotypic transformation, the morphological patterns and phenotypic change were similar to the tumor cell clusters in the invasive margin of the primary lesions.