PMC9348893_01
Pathology of Case one patient (A) H&E staining suggested lung adenocarcinoma (X400) (B) The result of IHC-Napsin A was positive (X400). (C) The result of IHC-TTF-1 was positive (X400).
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A puncture biopsy of the right mediastinal lymph node suggested lung adenocarcinoma ( Figure 1 ).
Zebrafish lung cancer model was established (A) Tumor cell proliferation was significantly reduced in the osimertinib group compared with the control group (B) Migration were significantly reduced in the osimertinib group compared with the control group. Whole-body image of the zebrafish embryo at 4 dpi (25x magnification). *(P<0.05), ***(P<0.001).
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Tumor cell proliferation and migration were significantly reduced in the osimertinib group compared with the control group ( Figures 2A, B ).
Chest computerized tomography (CT) scan images of patient one before and after one month of osimertinib treatment. (A) The images were before osimertinib treatment. (B) The images of were receiving osimertinib treatment after one month.
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According to RECIST v1.1, she achieved a partial response ( Figure 3 ) after 1 month of treatment, then unfortunately developed interstitial pneumonia.
PMC9348893_02
Pathology of Case two patient (A) H&E staining suggested lung adenocarcinoma (X100) (B) The result of IHC-Napsin A was positive (X400). (C) The result of IHC-TTF-1 was positive (X400).
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Cytologic evaluation of the pleural fluid was consistent with lung adenocarcinoma ( Figure 4 ).
Zebrafish lung cancer model was established (A) The proliferation of tumor cells were significantly reduced in the osimertinib group compared with the control group (B) The migration of tumor cells was not significantly reduced compared with the control group. Whole-body image of the zebrafish embryo at 4 dpi (25x magnification). **(P<0.01), ns: no significance.
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The proliferation of tumor cells in the osimertinib group was significantly reduced compared with the control group, and the difference was statistically significant ( Figure 5A ). The migration of tumor cells in the osimertinib group was not significantly reduced compared with the control group ( Figure 5B ), suggesting that osimertinib could be used as a treatment choice for this patient.
Chest computerized tomography (CT) scan images of patient two before and after one month of osimertinib treatment. (A) The images were before osimertinib treatment. (B) The images were receiving osimertinib treatment after one month.
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According to RECIST v1.1, she achieved stable disease ( Figure 6 ) and the pleural effusion was not controlled.