PMC4715337_01
Abdominal dynamic computed tomography. Abdominal dynamic computed tomography shows a huge liver tumor (14 cm in diameter) and right adrenal tumor (6 cm in diameter). a slice of liver tumor; b slice of right adrenal tumor
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Fig1
Abdominal computed tomography (CT) showed an internal heterogeneous right adrenal tumor (6 cm in diameter) with calcification as well as a huge liver tumor (14 cm in diameter) with internal necrosis (Fig. 1).
Histopathological diagnosis by examination of liver biopsy specimen. a Tumor cells show large conspicuous nuclei, nuclear atypicality, and acidophilic cytoplasm. Cells had a cord-like architectural pattern and alveolar structure (HE staining, original magnification x 400); b Normal hepatocytes (HE, x400); c These tumor cells are undyed; d Normal hepatocytes became stained (C, D: Hepatocyte paraffin 1 staining, x400); e-i Immunohistochemical stainings showed positive reactivity (E: Ad4BP/SF1, x40; F: Ad4BP/SF1, x400; G: 3beta-HSD, x40; H: P450c21, x40; I: Inhibinalpha, x40). Black arrow: Ad4BP/SF1 was stained in a nucleus. White arrow: steroidogenic enzymes was stained in cytoplasm
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Fig2
P450scc, 3beta-HSD, P450c21, P450c17 and DHEA-ST were all positive (Figs. 2 and 3).
Steroid hormone biosynthesis pathway. We applied immunohistochemical staining for P450scc, 3beta-HSD, P450c21, P450c17 and DHEA-ST
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Fig3
P450scc, 3beta-HSD, P450c21, P450c17 and DHEA-ST were all positive (Figs. 2 and 3).