PMC6491804_01
Maximum-likelihood phylogenies based on core-genome SNPs of 274 V. cholerae and three V. mimicus genomes. Colors have been grouped to reflect clade associations:Clade I (light green), Clade II (green), Clade III (yellow), Clade IV (blue), Clade V (orange), and Clade VI (red).
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F1
The phylogenetic analysis of 277 genomes of V. cholerae and V. mimicus divided them into six clades (Figure 1).
Geophylogeny of four sequenced V. cholerae strains. The numbers above the branches indicate the numbers of SNPs. Locations of the strains obtained from Anshan, Haicheng and Yingkou are indicated on the right side. The satellite imagery was generated by Global Mapper v20.
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F2
Piror to this finding we conducted a 6-month microbiological investigation of three sampling sites along the Liaohe River and identified 102 pathogenic strains from January to June 2018 (Figure S2), including two V. mimicus and three V. cholerae strains (Table 1). SNP differences at most, which represented an independent branch, differing strain BJG-01 by 290 SNPs (Figure 2). Strain AS-VC37 was clustered with HC-VC50 having 2 strain-specific SNPs, while YK-VC7 and clinical strain YK-VC11 formed another cluster with 2 SNP differences, indicating all sequenced V. cholerae strains belonged to the same clone.