PMC7523628_01
(a) Skin lesions present at the perineum and tail of a dog (arrows). (b) Gross diagnosis is severe extensive ulcerative haemorrhagic dermatitis (left recumbency, posterior view of lesions).
acmi-2-109-g001.jpg
PMC7523628_01_acmi-2-109-g001.jpg
F1
The gross skin lesions showed severe extensive ulcerative haemorrhagic dermatitis (Fig. 1a-b).
Cytologic finding of the lesion shows contorted branching septate fungal hypha. (Giemsa stain).
acmi-2-109-g002.jpg
PMC7523628_01_acmi-2-109-g002.jpg
F2
There were eosinophilic contorted septate hyphae, measuring 3-5 mum in diameter (Fig. 2).
Zoospores of P. insidiosum and zoospore germination shows short hypha (arrow) (bar=20 microm).
acmi-2-109-g003.jpg
PMC7523628_01_acmi-2-109-g003.jpg
F3
In this step, zoospore production was induced and two-flagellate zoospores were demonstrated (Fig. 3).
The phylogenetic tree was generated using neighbour-joining (NJ) analysis. Based on 10 000 replicates of bootstrap. (a) COX2 and (b) ITS region (*).
acmi-2-109-g004.jpg
PMC7523628_01_acmi-2-109-g004.jpg
F4
Each amplicon was analysed and used to construct the phylogenetic tree (Fig. 4a, b).