PMC6822057_01
a, b: Light microscopy shows no spike formation or bubbling in the glomerular capillary wall (periodic acid-methenamine-silver staining x 400). b: Enlarged image.
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Figure1
The functioning glomeruli showed no mesangial proliferation and no bubbling/spike appearance along the glomerular capillary walls (Figure 1).
Immunofluorescence staining for (a) IgG (gamma-heavy chain); b: IgA (alpha-heavy chain); c: IgA1 (alpha1-heavy chain); d: IgA2 (alpha2-heavy chain); e: IgM (micro-heavy chain); f: kappa-light chain; g: lambda-light chain; h: C3; i: C1q.
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Figure2
Immunofluorescence microscopy showed 2+ granular staining for IgA- and IgA1-heavy chains (Figure 2b, c), 2+ granular staining for kappa- and lambda-light chains (Figure 2f, g), and trace granular staining for C3 (Figure 2h) along the glomerular capillary walls, but no significant staining for IgG-, IgA2-, IgM-heavy chains, or C1q (Figure 2a, d, e, i).
a: Electron microscopy shows extensive foot process effacement of the podocytes, which contain large amounts of electron-dense materials mainly in the area covering the surface of the glomerular basement membrane. Bar = 10 microm. b: In higher magnification (inside the black square of Figure 3a), small amounts of electron-dense deposits are seen beneath the cytoplasm of the podocytes containing the dense materials (arrows). Bar = 1 microm.
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Figure3
Electron microscopy revealed extensive foot process effacement of the podocytes, which contained large amounts of dense materials mainly in the area covering the surface of the glomerular basement membrane, but not in the mesangial area (Figure 3a). Small amounts of electron-dense deposits were observed beneath the cytoplasm of the podocytes containing the dense materials (Figure 3b).