PMC4367051_01
Ulcer involving the left posterior lateral border of the tongue and lingual vestibule
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F1
Surface was covered with pseudo membranous slough, with normal tongue movement [Figure 1].
Neoplastic squamous cells infiltrating the connective tissue stroma (a-c) (viz. H and E, x4, x10 and x20) (d) with clear cytoplasm and centrally placed nucleus (H and E x40)
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F2
Neoplastic cells constituting plenty of lobules showed evidence of prominent clear cell changes [Figure 2a-d].
(a) Neoplastic lobules showing, intense positivity for cytokeratin AE1/AE3 (x20) (inset shows control tissue : oral mucosa, note the staining of epithelium alone), (b) Vimentin antibody showing negative reaction with neoplastic cells and positive reaction, in the tumor stroma (x40) (inset shows control tissue : oral mucosa, note the staining of connective tissue alone) (c) smooth muscle actin showing complete negative reaction with neoplastic cells and positivity along the smooth muscle lining of blood vessel wall (internal control) (x20) (inset shows control tissue : leiomyoma) and (d) homatropine bromide-45 antibody showing complete negative reaction with neoplastic cells and tumor stroma (x20) (inset shows control tissue : melanoma)
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F3
AE1/AE3 [Figure 3a] and for vimentin antigen intense positive reaction was seen only within the tumor stroma and the neoplastic cells showed negative reaction [Figure 3b]. Antibodies for SMA antigen showed complete negative reaction with neoplastic cells, but intense positivity was seen along the blood vessel lining [Figure 3c]. Antibodies for HMB 45 antigen showed complete negative reaction for both neoplastic cells and stroma [Figure 3d].