PMC9798227_01
Microscopic of gastroscopy biopsy tissue. (A) Gastroscopy showed a piece of irregular whitish focus in the big bend of the gastric antrum. (B) On low power, the gastric mucosa is mildly chronic inflammation, mildly active with erosion, and a large amount of eosinophilic sub-stance deposits in the stroma, 40x. (C) On medium power, the inherent glands of the stomach were destroyed, a few remained glands, and a large number of tissue cells containing crystals in the stroma were infiltrated, 200x. (D) On high power, needle crystals in cells, 400x.
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Gastroscopy revealed a white irregular lesion in the gastric mucosa at the big bend of the gastric antrum, which was soft and elastic ( Figure 1A ). Needle-shaped crystals were confined to the cytoplasm, and some were in parallel arrays ( Figures 1B-D ). Immunohistochemically, these crystal-containing cells were strongly positive for CD68/PGM1 ( Figure 2A ), but negative for CD20 ( Figure 2B ), CD138 ( Figure 2C ), CD79a ( Figure 2D ), CK ( Supplementary Figure 1A ), S-100 ( Supplementary Figure 1B ), desmin, smooth muscle actin (SMA), muscle-specific actin (MSA), and myosin. Helicobacter pylori (H. pylori,  Supplementary Figure 1C ) immunostaining was negative.
Immunophenotype and electron microscopic manifestations of gastroscopy biopsy tissue. (A) CD68, 400x; (B) CD20, 400x; (C) CD138, 400x; (D) CD79a, 400x; (E) kappa light chain, 400x; (F) Electron microscopic examination showed that a large number of high electron density particles were found in the cytoplasm of cells, with crystal structures with different sizes and shapes, 1000x.
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Immunohistochemically, these crystal-containing cells were strongly positive for CD68/PGM1 ( Figure 2A ), but negative for CD20 ( Figure 2B ), CD138 ( Figure 2C ), CD79a ( Figure 2D ), CK ( Supplementary Figure 1A ), S-100 ( Supplementary Figure 1B ), desmin, smooth muscle actin (SMA), muscle-specific actin (MSA), and myosin. Immunostaining of kappa light chain was strong and diffuse ( Figure 2E ). Electron microscopic examination showed numerous high-electron-density particles in the cytoplasm of cells, with crystal structures with different sizes and shapes ( Figure 2F ).