PMC6497792_01
Impact of severe drought on the obtained morphological traits. Variation in the genetic response of survival and recovery rates, the response of genotypes was classified into recovering and retarding responses (A), days to heading (B), awn length (C), peduncle length (D), and plant height alleles (E).
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F3
At harvest, morphological traits (Plant height, peduncle length, awn length, root length, and root dry weight), plant biomass (standing biomass and total plant dry weight), and plant yield (number of tillers, the total spike number, spike weight, number of productive tillers, grain number, and grain weight) were measured (Figure S3).
Impact of severe drought on: Root traits including the reduced overall mean of total root length biomass (A), and the individual total root dry weight of genotypes (B). Plant biomass through the average of relative plant weight (C), and the genetic variation of the standing biomass (D). The overall reduction of grain number (E), and the variable response of grain yield represented by grain weight among the genotypes (F).
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F4
The description of both rates is illustrated here (Figures S4A,B).
Representative images of different response to the drought. (A) An adaptive plant represents Onas, associated with the minimal impact of drought. (B) Moderate adaptation by the Sakha94, smaller spikes with shorter awns, and shorter roots. (C) Limited adaptation of Louise, limited recover, a significant loss in yield and spike shrinkage and rolling associated with significant reduction in root biomass. (D) Susceptibility to drought by Alpowa, abnormal spike, and lack of yield and dead roots.
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F5
Here, we advanced the technique to sample into the Deep 96-well-collection plates (2 ml) in liquid nitrogen to match the high-throughput format (Figure S5).