PMC3325743_01
A composite panel of four pictures showing the characteristic histological features of the pleomorphic adenoma in this case: A) a low power magnification of the lesion shows the mass with a variable cellularity and fibrous trabeculae (Hematoxylin and Heosin, 4x objective); B) at a high power, the lesion shows somewhat uniform appearing epithelial cells arranged in tubules as small nests set in a chondromyxoid to fibrous stroma (Hematoxylin and Heosin, 20x objective); C) the epithelial cells are positive for pankeratin by immunohistochemistry (AE1/AE3, 20x objective); D) the myoepithelial cells cytoplasm is focally positive for glial fibrillary acidic protein by immunohistochemistry (glial fibrillary acidic protein, 60xobjective).
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F1
Histologically, the mass showed a fibrous and chondromyxoid stroma containing epithelial and myoepithelial cells (Figure 1A). The epithelial component consisted of relatively uniform appearing cells arranged in tubules and small nested aggregates (Figure 1B). The epithelial component showed positivity for keratin AE1/AE3 (Figure 1 C), CAM5.2 antigen, epithelial membrane antigen (EMA, focal), carcinoembryonic antigen (CEA, focal), and C-KIT gene (scattered). The myoepithelial cells were focally positive for S100 and glial fibrillary acidic protein (GFAP) as shown in Figure 1D.