PMC6324099_01
(a) Slit lamp photograph of the right eye in diffuse illumination showing a central ring-shaped anterior to mid stromal dry infiltrate (4 mm x 5 mm) with dense focus of infiltrate within the ring. (b) Slit lamp photograph of the right eye with sclerotic scatter highlighting the central scar
IJO-67-150-g001.jpg
PMC6324099_01_IJO-67-150-g001.jpg
F1
The cornea had an epithelial defect with an underlying anterior to mid-stromal ring-shaped infiltrate with hyphate edges measuring 5 x 4 mm (Haag Streit, BM 900, USA) and a smaller dense central infiltrate within [Fig. 1a]. Natamycin (5%) eye drops half hourly, tapered fortnightly to 8 times a day and continued 2 weeks beyond complete resolution with oral ketoconazole 200 mg twice daily for 6 weeks cured her infection in 4 months [Fig. 1b], At the last follow-up, her unaided visual acuity in the right eye was 20/200 with no further improvement owing to the central scar.
(a) Pseudopestalotiopsis theae conidiomata sporulating on potato dextrose agar at day 20. (b) Lactophenol cotton blue mount (1000x magnification) showing fusoid 4-septate conidia with slight constrictions at the septae and conical basal cell with truncated base. The 3 median cells are concolorous and brown with darker colored septae. The conical apical cells have filiform branched appendages. (c) ITS sequence analysis generated a phylogram which shows that our sample GMRV-L 825/15 has 99% similarity with Pseudopestalotiopsis theae
IJO-67-150-g002.jpg
PMC6324099_01_IJO-67-150-g002.jpg
F2
Further, incubation of PDA showed black shiny dots in the center with irregular surface [Fig. 2a], Lactophenol cotton blue mount showed brown septate hyphae and characteristic 5-celled brown spores with fine appendages at the tip [Fig. 2b], resembling spores of Pestalotiopsis.