PMC9884323_01
A well-defined spindle-shaped mass in the anterior intermuscular compartment of proximal right leg involving tibialis anterior and right extensor digitorum longus muscles. (a) The lesion shows intermediate signal intensity with few hyperintense foci on T1-weighted sequence, (b) heterogeneous hyperintensity on T2-weighted, (c) short Tau inversion recovery sequences, and (d) heterogenous post-contrast enhancement.
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The lesion was abutting the right proximal tibia with thinning of its cortex, abutting and compressing anterior crural fascia, tibialis posterior muscle and its tendon, tibialis anterior vessels, and interosseous membrane [Figure 1].
Fine needle aspiration cytology showing spindled cells (red arrow) with high nuclear-to-cytoplasmic ratio, clumped chromatin, and prominent nucleoli (Giemsa staining, magnification = 100 x).
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It revealed low cellular smears, chiefly consisting of spindled cells arranged mostly singly with a high nuclear-to-cytoplasmic ratio, clumped chromatin, and prominent nucleoli [Figure 2].
Section showing highly cellular tumor cells mixed with focal areas of hemorrhagic cyst-like spaces (red arrow) (Hematoxylin and eosin staining, magnification = 40 x).
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Hematoxylin and eosin stained sections showed highly cellular tumor cells mixed with focal areas of hemorrhagic cyst-like spaces [Figure 3].
Section showing abundant chronic inflammatory cells (red arrow) (Hematoxylin and eosin staining, magnification = 100 x).
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There was also the presence of abundant chronic inflammatory cells [Figure 4].
Section showing tumor cells with pleomorphism and mitoses (red arrows) (Hematoxylin and eosin staining, magnification = 100 x).
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There was no evidence of vascular and neural invasion [Figure 5].
Positive staining for vimentin (magnification = 100 x).
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Antibody, Cell Marque) [Figure 6] and negative for desmin (EP15, 1:100, Rabbit Monoclonal Primary Antibody, Cell Marque) [Figure 7] and S100 (Anti-human S-100 protein, 15E2E2, Purified Bovine S-100 Protein, Biogenex) [Figure 8]. A diagnosis of AFH was made based on histopathology and IHC findings.
Negative staining for desmin (magnification = 100 x).
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Antibody, Cell Marque) [Figure 6] and negative for desmin (EP15, 1:100, Rabbit Monoclonal Primary Antibody, Cell Marque) [Figure 7] and S100 (Anti-human S-100 protein, 15E2E2, Purified Bovine S-100 Protein, Biogenex) [Figure 8]. A diagnosis of AFH was made based on histopathology and IHC findings.
Negative staining for S-100 (magnification = 100 x).
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Antibody, Cell Marque) [Figure 6] and negative for desmin (EP15, 1:100, Rabbit Monoclonal Primary Antibody, Cell Marque) [Figure 7] and S100 (Anti-human S-100 protein, 15E2E2, Purified Bovine S-100 Protein, Biogenex) [Figure 8]. A diagnosis of AFH was made based on histopathology and IHC findings.