PMC10242137_01
Chest x-ray at day of birth (A) showing mild diffuse granular opacification with progression of lung disease on day of life 5 (B) and 10 (C).
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F1
CPAP and FiO2 0.21. Chest x-ray showed mild diffuse hazy granular opacities consistent with respiratory distress syndrome (Figure 1A). Chest x-ray at this point showed diffuse bilateral hazy opacities with increased perihilar markings (Figure 1B). Chest x-ray now showed bilateral interstitial infiltrates (Figure 1C).
Immunostaining for lung (A) and heart (B) sections for low-density lipoprotein receptor (LDLR), nuclear factor kappa beta (NF-kb), citrullinated histone H3 (citH3), osteopontin (OPN), and intercellular adhesion molecule 1 (ICAM1).
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F2
We found, specifically, increased low-density lipoprotein receptor (LDLR) and phospo-NF-kB-p65 expression in lung macrophages and cardiomyocytes, increased osteopontin (OPN) and intracellular adhesion molecule 1 (ICAM-1) in blood vessels in the lung and heart (Figure 2), and increased citrullinated histone H3 (citH3) in the lung and blood vessels in the heart (Figure 2), indicating hyperinflammation with formation of NETs.