PMC7223195_01
Visual examination revealed a red-brown erosion of the glans penis around the external urethral orifice.
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Visual examination revealed red-brown erosion on the glans penis around the external urethral orifice (Figure 1).
(a) Growths of stratified squamous epithelial cells with nuclear atypia in all layers of the glans mucosa are shown, and a diagnosis of penile BD was made (H&E stain, 40x magnification). (b) ISH analysis revealed the widely observed punctate HPV16 DNA signals (brown staining) in the nuclei of cancer cells (100x magnification). (c) Immunohistochemistry revealed that p16-INK4a was widely expressed in the tumor tissue (40x magnification).
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No definite BD lesions in the distal urethra were expected to be found in this case; therefore, local tumor resection with skin grafting from the femoral skin was performed (Figure 2(a)). In addition, the locations of HPV16 DNA in the tumor tissue could be observed with in situ hybridization (ISH) (Figure 2(b)). Immunohistochemistry demonstrated that p16-INK4a, which is a surrogate marker of oncogenic HPV-E7 protein, was widely expressed in the tumor tissue (Figure 2(c)).