PMC9133757_01
The lymph node biopsy immunohistochemistry in Case 1 at diagnosis.. Immunohistochemistry of the lesion biopsy: MPO (+), CD68/PGM-1 (+), CD43 (+), CD56 (scattered+), CD20 (-), CD3epsilon (-), CD4 (-), CD8 (-), CD30 (-), CD99 (-), CD123 (-), EMA (-), ALK-1 (-), granzyme B (few+), TIA-1 (part+), EBER1/2 in situ hybridization (-).
gr1.jpg
PMC9133757_01_gr1.jpg
fig0001
Immunohistochemical (IHC) staining showed as follows: MPO (+), CD68/PGM-1 (+), CD43 (+), CD56 (scattered+), CD20 (-), CD3epsilon (-), CD4 (-), CD8 (-), CD30 (-), CD99 (-), CD123 (-), EMA (-), ALK-1 (-), granzyme B (few+), TIA-1 (part+), EBER1/2 in situ hybridization (-) (Fig.1).
PMC9133757_02
The host microchimeric amplification in Case 2.. N5-1(+) means the rs610937 locus insertion of the donor, and N7-1(-) means the deletion of rs16458 locus.
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PMC9133757_02_gr3.jpg
fig0003
In August 2020, the donor cells in patients microchimerism accounted for 0.005% (Fig.3).