PMC5626218_01
- A) Desquamation and crusts in the trunk; B) 1- purulent bulla, 2,3- not
umbilicated clear blisters.
1678-9946-rimtsp-S1678-9946201759041-gf01.jpg
PMC5626218_01_1678-9946-rimtsp-S1678-9946201759041-gf01.jpg
f01
Since 2013 he has been submitted to oral prednisone (1 mg/kg/day) (Figure 1). In 2016, examination of a Gram-stained smear of aspirated material from the purulent bulla (Figure 1, B1) yielded a positive culture for Staphylococcus aureus; two swabs for PCR were taken from non purulent bullous lesions in the trunk (Figure 1, B2, B3).
- Ethidium bromide stained agarose gel showing the 244 bp HSV amplification
product and the 220 bp BAC amplification product (segment of the human
beta-Actin gene used as PCR positive control) and the size marker 100 bp
(Invitrogen).
1678-9946-rimtsp-S1678-9946201759041-gf02.jpg
PMC5626218_01_1678-9946-rimtsp-S1678-9946201759041-gf02.jpg
f02
In 2016, examination of a Gram-stained smear of aspirated material from the purulent bulla (Figure 1, B1) yielded a positive culture for Staphylococcus aureus; two swabs for PCR were taken from non purulent bullous lesions in the trunk (Figure 1, B2, B3). Human Herpesvirus (HHV), in both PCR assays (Figure 2), but we could not distinguish between HSV1 or 2 (herpes simplex virus 1 or herpes simplex virus 2). HSV PCR products were then purified by GE Healthcare commercial kit and the fragments (sense and anti-sense) were sequenced using the Big DyeTM terminator sequencing protocol (Applied Biosystems Inc., Foster City, CA).