PMC7226562_01
Expression of CD11a/CD18 and CD11b/CD18. Peripheral blood mononuclear cells were obtained from whole blood. Cells were stained ex vivo for surface markers, with fluorescently conjugated anti-CD18 PE and anti-CD11a FITC and CD11b PECy5. Samples were acquired with BD FACS Calibur and analyzed using FlowJo software v. 9.810.2. For data analysis, granulocytes were gated on Forward scatter and Side scatter, and cells were checked for the expression of CD11a/CD18 and CD11b/CD18
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F1
The frequency of granulocytes having dual expression of CD11a/CD18 and CD11b/CD18 was below 1% (Fig. 1). Presently, the child is healthy at two years post-transplant, and all his laboratory parameters including CD11a/CD18 and CD11b/CD18 expression on granulocytes have normalized (Fig. 1).