PMC7523625_01
PMC7523625_02
PMC7523625_03
Culture characteristics of the 
Bacillus clausii
 isolate from blood on (a) blood agar (colony variants) and (b) chocolate agar.
acmi-2-097-g001.jpg
PMC7523625_03_acmi-2-097-g001.jpg
F1
Gram-positive bacilli within 48 h which were isolated in pure culture and identified as  B. clausii  by a matrix assisted laser desorption ionization time of flight (MALDI-TOF; bioMerieux) assay (Fig. 1). Growth was observed 11 days after initiation of the probiotic containing spores of  B. clausii .
Antibiotic susceptibility testing of the clinical isolate (right) and probiotic strain (left)
acmi-2-097-g002.jpg
PMC7523625_03_acmi-2-097-g002.jpg
F2
We were able to isolate and identify the organism as  B. clausii  using MALDI-TOF with the susceptibility pattern of the probiotic strain perfectly matching the blood isolate (Fig. 2).