PMC5574471_01
Bilateral diffuse miliary nodular infiltrates with mediastinal and bilateral hilar adenopathy.
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PMC5574471_01_10.1177_1179547617724776-fig1.jpg
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Computed tomography of the chest, abdomen, and pelvis revealed miliary pulmonary pattern, patchy nodular infiltrates, and mediastinal lymphadenopathy with peritoneal and omental involvement (Figure 1).
Flow cytometric gating strategy for detection of percentage of activated T cells (CD3+CD4+ and CD3+CD8+) co-expressing CD25+CD134+ markers. (A) Viable lymphocyte gate using side and forward scatter, (B) gate on CD3+/CD4+, (C) CD3+CD4+/CD25+CD134+ co-expression after 48 hours incubation with an unstimulated sample, PPD, and ESAT-6/CFP-10 peptides mix (RD1 peptide antigen), (D) gate on CD3+/CD8+, (E) CD3+CD8+/CD25+CD134+ co-expression after 48 hours incubation with an unstimulated sample, PPD, and RD1 peptide antigen. Percentages (boxes) indicate the calculated distribution of CD25+CD134+ among CD3+CD4+ and CD3+CD8+ T cells after the subtraction of background (nil). FCS-A indicates forward scatter; PPD, purified protein derivatives; RD1, region of difference 1; SSC-A, side scatter.
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CD25+CD134+ co-expression was detected on 0.34% and 0.84% of RD1 peptide and PPD-stimulated CD3+CD4+ T cells, respectively (Figure 2).