PMC10064289_01
Pathological features and FISH analysis of MCL and cHL components, respectively. Hematoxylin and eosin (HE) staining (A) shows that MCL is composed of monomorphic small lymphocytes which resembling centrocytes, these cells show positive immunohistochemistry staining for CD5 (B), CyclinD1 (C), and PAX-5 (D). The proliferation rate of Ki-67 is approximately 40% (E). FISH analysis shows CCND1 gene break (F) as well as IgH/CCND1 gene fusion (G) in MCL. In cHL region, HE staining (H) demonstrates that cHL component consists of mixed inflammatory cells and large HRS cells. HRS cells show positive immunohistochemistry staining for CD30 (I), CD15 (J), PAX-5 (K) and the proliferation rate of Ki-67 is approximately 70% (L). In HRS cells, FISH analysis dose not demonstrate CCND1 gene break (M) and IgH/CCND1 gene fusion (N).
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F1
One component consisted of monomorphic small lymphocytes with a vague nodular pattern (Figure 1A). They weakly expressed CD5 (Figure 1B) and were positive for CyclinD1 (Figure 1C), PAX-5 (Figure 1D), CD20, OCT-2, and BOB-1. The proliferation rate of Ki-67 was approximately 40% (Figure 1E). The other component of the lymph node showed scattered large mononuclear Hodgkin cells and binuclear or multinuclear Reed-Sternberg cells in a background of mixed inflammatory cells (Figure 1H). Immunochemistry revealed positive staining of Hodgkin and Reed-Sternberg (HRS) cells for CD30, CD15, and PAX 5 (Figures 1I-K); decreased expression of CD20, OCT-2, and BOB-1; and negative expression of CD5 and CyclinD1. The proliferation rate of Ki-67 in the HRS cells was approximately 70% (Figure 1L). MCL cells (Figure 1F) but not in the cHL cells (Figure 1M) using FISH gene break detection (Abbott, Des Plaines, United States). Moreover, immunoglobulin heavy chain (IGH)/CCND1 fusion translocation was observed in the MCL cells (Figure 1G) but not in the cHL cells (Abbott, Des Plaines, United States) (Figure 1N).