PMC3325754_01
Histolopathology of the tumor. A) Very low power view shows vascular tumor measuring 1x1x1 cm, Haematoxylin & Eosin, x10; B) low power view of the tumor shows pericytomatous pattern, Haematoxylin & Eosin, x 100; C) high power view of the tumor shows ooal to spindle tumor cells with hyperchromatic nuclei located around the vessels. Mitotic figures are scattered (arrows). Haematoxylin & Eosin, x200.
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F1
Grossly, the tumor was reddish, and measured 1x1x1 cm (Figure 1A). Microscopically, oval to spindle tumor cells with hyperchromatic vesicular nuclei and many vasculatures were seen (Figure 1B and 1C). The tumor cells were contiguous and mixed with endothelial cells in many blood vessels, thus resembling pericytes (Figure 1B and 1C). Mitotic figures were scattered (Figure 1C).
The tumor cells are positive for alpha-smooth muscle actin (A), p53 (B), and Ki67 (labeling=40%), but negative for CD34 (C). The blood vessels' endothelial cells are positive for CD35 (D). Immunostaining, x200.
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F2
Immunohistochemically, the tumor cells were positive for vimentin, alpha-smooth muscle actin (Figure 2A) and p53 (Figure 2B). The Ki67 labeling was 40% (Figure 2C). The tumor cells were negative for cytokeratins (AE1/3 and CAM5.2), CD31, CD34 (Figure 2D), S100 protein, desmin, HMB45, CD10, vimentin, and factor VIII-related antigen. The endothelium of the vessels were positive for vimentin, CD31, CD34 (Figure 2D) and factor VIII-related antigen, but negative for alpha-smooth muscle actin, p53, cytokeratins (AE1/3 and CAM5.2), S100 protein, HMB45, CD10, vimentin, and desmin.