PMC9397586_01
Clinical and histopathological studies of the LPPi and LP subjects. A. LPPi axilla lesions in Subject #1. B. LPPi groin lesions in Subject #2. C. LPPi lesional biopsy from Subject #2. Note the vacuolar degeneration of the epidermal basal cell layer, band-like lichenoid or perivascular lymphocytic infiltrates in the papillary dermis as well as superficial pigmentary incontinence and melanophages. (20X magnification). D. LP lesions on the right lower extremity. E. LP lesional biopsy. Note the hyperkeratosis, acanthosis, the sawtooth epidermal appearance, and the band-like dermal lymphohistiocytic infiltrate (10X magnification).
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F1
Physical exam revealed scattered erythematous and violaceous papules and plaques on the bilateral feet and ankles (Figure 1 D). The superficial dermis was also notable for a predominately lymphohistiocytic infiltrate (Figure 1 E). Supplemental Figures S1-3 detail the placement of devices for the three subjects. There is significant downregulation of oxidative phosphorylation/cellular respiration pathways. The downregulated gene set is largely composed of mitochondrial transcripts that code for components of the electron transport chain (Figure 2 and Supplementary Table S1).
PMC9397586_02
Clinical and histopathological studies of the LPPi and LP subjects. A. LPPi axilla lesions in Subject #1. B. LPPi groin lesions in Subject #2. C. LPPi lesional biopsy from Subject #2. Note the vacuolar degeneration of the epidermal basal cell layer, band-like lichenoid or perivascular lymphocytic infiltrates in the papillary dermis as well as superficial pigmentary incontinence and melanophages. (20X magnification). D. LP lesions on the right lower extremity. E. LP lesional biopsy. Note the hyperkeratosis, acanthosis, the sawtooth epidermal appearance, and the band-like dermal lymphohistiocytic infiltrate (10X magnification).
nihms-1789618-f0001.jpg
PMC9397586_02_nihms-1789618-f0001.jpg
F1
Physical exam revealed scattered erythematous and violaceous papules and plaques on the bilateral feet and ankles (Figure 1 D). The superficial dermis was also notable for a predominately lymphohistiocytic infiltrate (Figure 1 E). Supplemental Figures S1-3 detail the placement of devices for the three subjects. There is significant downregulation of oxidative phosphorylation/cellular respiration pathways. The downregulated gene set is largely composed of mitochondrial transcripts that code for components of the electron transport chain (Figure 2 and Supplementary Table S1).
Heat map of differentially expressed genes with markers grouped by unsupervised hierarchical clustering.. Unit variance is by row; blue color indicates lower mean expression and red color indicates higher mean expression. Columns are non-lesional skin (NLS), Lichen Planus Pigmentosus lesional skin (LPPi), and Lichen Planus lesional skin (LP). Fold changes for lesional (LS) vs NLS as well as LPPi vs LP. *P < 0.05; **P<0.01; *** P<0.001.
nihms-1789618-f0002.jpg
PMC9397586_02_nihms-1789618-f0002.jpg
F2
Supplemental Figures S1-3 detail the placement of devices for the three subjects. There is significant downregulation of oxidative phosphorylation/cellular respiration pathways. The downregulated gene set is largely composed of mitochondrial transcripts that code for components of the electron transport chain (Figure 2 and Supplementary Table S1).
Volcano plot of differentially expressed genes in LP and LPPi.. Red dots indicate the 543 genes that have a p<0.05, only 15 of these were preferentially expressed in LPPi. Grey dots indicate genes with a less than 1-fold change.
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PMC9397586_02_nihms-1789618-f0003.jpg
F3
Supplemental Figures S1-3 detail the placement of devices for the three subjects. As denoted in the volcano plot in Figure 3, LPPi vs LP gene expression analysis produced 543 differentially expressed genes (p<0.05) - only 15 of which were preferentially expressed in LPPi; LP, the prototypical lichenoid skin reaction, seems to be far more active as an inflammatory process.