PMC7530177_01
CD4+ T cells from the MUL child are reduced in number and expressed low levels of intracellular TRIM37. (A) Flow cytometry plots show CD4+ and CD8+ cell frequency in peripheral blood mononuclear cells (PBMCs) from the MUL child and matched healthy control. Numbers in plots indicate positive cells. Data are from one representative experiment out of six. (B) Flow cytometry plots show the Annexin V and Propidium Iodide (PI) staining in CD4+ (upper panels) and CD8+ (lower panels) T cells from the MUL and healthy child. Numbers in plots indicate positive cells. Data are from one representative experiment out of three. (C) Flow cytometry plots show Annexin V and PI in 48 h TCR-stimulated CD4+ (upper panels) and CD8+ (lower panels) T cells from the MUL and healthy child. Numbers in plots indicate positive cells. Data are from one representative experiment out of two. (D) The upper panel shows representative immunoblot for TRIM37 and actin proteins in unstimulated and 30 min TCR-stimulated CD4+ T cells from the MUL and healthy child. The lower panel shows the relative densitometric quantitation of the TRIM37 protein normalized on actin in CD4+ T cells from the MUL and healthy child, in the aforementioned experimental conditions. Data are shown as mean +- SEM (n = 4 densitometric quantitation derived from four films with different exposure timing). **P < 0.01; ****P < 0.0001 by unpaired two-tailed ordinary 2-way ANOVA corrected with Bonferroni's multiple comparisons test. (E) The upper panel shows representative immunoblot for TRIM37 and actin proteins in unstimulated and 30 min TCR-stimulated CD8+ T cells from the MUL and healthy child. The lower panel shows the relative densitometric quantitation of TRIM37 protein normalized on actin in CD8+ T cells from the MUL and healthy child, in the aforementioned experimental conditions. Data are shown as mean +- SEM (n = 4 densitometric quantitation from four films with different exposure timing). NS, not significant by unpaired two-tailed ordinary 2-way ANOVA corrected with Bonferroni's multiple comparisons test.
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T cells compared with sex-age matched healthy subjects (Table 1 and Figure 1A). The MUL child also displayed an increased frequency of both CD8+ T cells and double positive CD4+CD8+ T lymphocytes in peripheral blood (Table 1 and Figure 1A).