PMC5738886_01
Left pheochromocytoma (arrows); dog. Neoplastic vascular invasion (asterisk) in caudal vena cava (CVC) and abdominal phrenic vein (APV). Left kidney (LK). Right kidney (RK).
OpenVetJ-7-332-g001.jpg
PMC5738886_01_OpenVetJ-7-332-g001.jpg
F1
Abdominal ultrasound revealed a mass in the left adrenal gland (confirmed by necropsy, 4 cm in diameter) with tumor invasion of the abdominal phrenic vein (APV) and caudal vena cava (CVC), which prompted the decision of euthanasia (Fig. 1), and a whitish nodule in the right adrenal gland (0.5 cm in diameter); macroscopically, thinning of the adrenal cortex was observed (Fig. 2).
Right pheochromocytoma (asterisk), sagittal section; dog. Thinning (between arrows) of adrenal cortex (AC). Adrenal medulla (AM).
OpenVetJ-7-332-g002.jpg
PMC5738886_01_OpenVetJ-7-332-g002.jpg
F2
Abdominal ultrasound revealed a mass in the left adrenal gland (confirmed by necropsy, 4 cm in diameter) with tumor invasion of the abdominal phrenic vein (APV) and caudal vena cava (CVC), which prompted the decision of euthanasia (Fig. 1), and a whitish nodule in the right adrenal gland (0.5 cm in diameter); macroscopically, thinning of the adrenal cortex was observed (Fig. 2).
Parathyroid adenoma (asterisk); dog. Caudal parathyroid gland (Pc) and left thyroid lobe (LTL).
OpenVetJ-7-332-g003.jpg
PMC5738886_01_OpenVetJ-7-332-g003.jpg
F3
The left cranial parathyroid gland was enlarged (0.7 x 0.6 cm) (Fig. 3); accordingly, PTH plasma determination was requested, together with a new assessment of total calcium (pre-euthanasia sample).
Parathyroid chief cell adenoma; dog. Section showing cubic cells arranged in tortuous trabeculae and packets supported by a delicate fibrovascular stroma (HE).
OpenVetJ-7-332-g004.jpg
PMC5738886_01_OpenVetJ-7-332-g004.jpg
F4
Cells formed tortuous trabeculae and packets supported by a delicate fibrovascular stroma with multiple capillaries containing numerous erythrocytes (Fig. 4).
Right pheochromocytoma; dog. Section showing large polyhedral cells forming irregular packets, delimited by a delicate fibrovascular stroma. HE. Smaller box: section of adrenal cortex not invaded by pheochromocytoma, with traces of zona glomerulosa (Zg) and atrophy of zona fasciculata (Zf) and zona reticularis (Zr). Capsule (C) (HE).
OpenVetJ-7-332-g005.jpg
PMC5738886_01_OpenVetJ-7-332-g005.jpg
F5
Cells were organized in aggregates, forming irregular packets, delimited by a delicate fibrovascular stroma derived from a thicker fibrous capsule (Fig. 5). In the right adrenal gland, in those areas not invaded by the neoplasia, traces of zona glomerulosa with atrophy of zona fasciculata and zona reticularis were observed (Fig. 5, smaller box).
Left pheochromocytoma; dog. Section showing more than 90% of cells with positive staining to anti-synaptophysin antibody in histochemical procedures (Avidin-biotin complex). Smaller box: Strongly positive cytoplasmatic staining of neoplastic cells (Avidin-biotin complex).
OpenVetJ-7-332-g006.jpg
PMC5738886_01_OpenVetJ-7-332-g006.jpg
F6
In more than 90% of the cells comprising both neoplasias, the cytoplasm was strongly positive to anti-synaptophysin antibody (Fig. 6).