PMC10189013_01
Course of the disease.
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f1
The staging revealed stable findings ( Figure 1 ).
Summary of tumor variants monitored in plasma cfDNA. (A) Selected tumor variants detected by tumor normal sequencing with a comprehensive sequencing panel. Resulting variants were selected for a smaller tumor-specific hybridization-based enrichment panel and sequenced to an ultra-high depth. Allele frequencies (AF) were obtained from reference reads (REF, GRCh38) and alternative reads (ALT). Reads without duplicates were excluded during sequencing error correction. (B) ctDNA kinetics of selected tumor variants. Each line represents one variant. The allele frequencies are plotted over time and refer to reads with at least one duplicate. White circles indicate that the variant was found with a p-value < 0.05.
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In accordance with the clinical findings and imaging results, the allele frequencies were markedly reduced at time point 2 ( Figure 2B ).