PMC3754489_01
(A) Microscopic observation of numerous acid fast bacilli (red rods) in the slit skin smear fluid from a solitary lesion in the face at the initial presentation of the patient. Original magnification: x1,000. (B) primers used in this study. The letters F and R refer to forward and reverse primer, respectively. (C) Amplification products of folP, gyrA, and rpoB genes (lanes 1-3, respectively). (D) A mutation of codon 441 of the rpoB gene was found (GAt AAC) (indicated in red).
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Acid-fast bacilli were isolated from a skin biopsy specimen (Figure 1A). Amplification and DNA sequencing of folP1, rpoB, and gyrA genes - involved in resistance to DDS, RFP, and quinolones, respectively - were also performed (Figure 1C). Sequence analysis of these genes revealed a mutation within codon 441 of the rpoB gene (GAT AAC), which indicates that it is the third reported strain harboring this mutation but a new two-base substitution (Figure 1D).