PMC5470563_01
- Photomicrography of the testis tumor (H&E). A - Panoramic view showing the neoplasia with cystic and solid areas, (100X); B - Details of the neoplastic cells, with rounded hyperchromatic nuclei and visible nucleoli, and occasional nuclear infoldings, (400X); C and D - Neoplastic cells arranged in follicular-like structures enclosing watery basophilic substance (C 200X, D 100X).
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The morphology was characterized by solid areas, and focal cystic structures resembling follicles containing a light basophilic watery substance (Figure 1A, 1C and 1D). The solid areas predominated and showed a lobulated arrangement formed by large cells with light eosinophilic cytoplasm, with rounded hyperchromatic nuclei and visible nucleoli, besides occasional nuclear infolding (Figure 1B).
- Photomicrography of the testis tumor. Immunohistochemistry. A - Calretinin positivity in neoplastic cells (200X); B - Diffuse vimentin positivity in neoplastic cells (400X); C - Focal inhibin positivity (200X); D - Smooth muscle actin (SMA) positivity highlighting the lobular pattern of the tumor (100X).
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Immunostainings revealed diffuse positivity for vimentin and focal calretinin and inhibin, and negativity for beta-hCG, alpha-fetoprotein and cytokeratins (Figure 2), thus ruling out yolk sac tumor, which was the major differential diagnosis.