PMC6411643_01
Brain MRI. (A) Coronal and (B) transversal slice of T1-weighted brain imaging revealed moderate temporal lobe atrophy (medial temporal lobe atrophy score 2) and white matter lesions (Fazekas grade 2). We did not find any indication for inflammatory processes in FLAIR, T2- and Diffusion weighted sequences.
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F1
In the local hospital, brain MRI showed a moderate temporo-parietal atrophy (medial temporal lobe atrophy score 2, Figure 1).
Immunoreactivity of CSF. Indirect immunostaining of CSF with unfixed mouse brain sections showing signal to neuropil in the (A) thalamus, (B) olfactory bulb, and (C) cerebellum. CSF reactivity is restricted to a subset of granule cells (GL) in the cerebellum (C, insert), as compared to the dense neuronal layer seen with H&E staining (D) or the neuronal marker NeuN (E) of adjacent sections. (ML, molecular layer; WM, white matter).
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F2
Still suspecting an autoimmune process, CSF and serum were incubated on mouse brain sections revealing strong antibody reactivity on neuropil and neuronal surface targeting a still unknown epitope in the cerebellum, thalamus, hippocampus, and the olfactory bulb (Figure 2).