PMC3331883_01
Skin-colored, elastic-hard, well-demarcated nodule on the right cheek. The tumor mass was 20 x 22 mm in size.
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F1
On the initial visit, physical examination revealed a skin-colored, elastic-hard, well-demarcated nodule on the right cheek (fig. 1).
Cords, lobules, and interconnected strands with peripheral, palisaded border of basaloid-like cells and a slight admixture of central mononuclear cells (original magnification a x50, b-d x200).
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F2
Histologic examination revealed cords, lobules, and interconnected strands with a peripheral, palisaded border of basaloid-like cells and a slight admixture of central mononuclear cells (fig. 2a). In addition, the tumor stroma was positive for Alucian blue (fig. 2b), and the inside of the duct was positive for AE1/AE3 (fig. 2c). We also determined that the cells composing the tumor lobules were partially positive for BerEP4 (fig. 2d).
Immunohistochemical stainings for cutaneous lymphadenoma. Paraffin-embedded tissue samples from the patient were stained as follows: the sections were developed with 3,3-diaminobenzidine tetrahydrochloride for CD1a (a), CD3 (b), CD4 (c), CD7 (d), CD8 (e), CD20 (f), CD68 (g), and TIA-1 (h) (original magnification x200).
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F3
TIA-1+ T cells (fig. 3b-e, h). These tumor-infiltrating T cells were mainly CD45Ro+ memory T cells, and several CD45RA+ cells were also detected (data not shown). CD68+ cells were also detected within and around the lobules (fig. 3f, g). CD56+ cells were not detected in our case (data not shown).
Formalin-fixed paraffin-embedded tissue samples from patients with cutaneous lymphadenoma were sectioned at 4 mum and deparaffinized. Deparaffinized sections were treated with autoclave heating at 121 C for 5 min. After pretreatment, the sections were stained with an anti-Foxp3 Ab. The sections were developed with new fuchsin. Arrowheads indicate Foxp3+ cells (original magnification x200).
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F4
In addition, several Foxp3+ Tregs were detected within the lobules (fig. 4).