PMC4284984_01
Nests of poorly differentiated squamous cells with interspersed osteoclastic giant cells. H&E stain, x50.
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fig1
Microscopically the tumor was composed of infiltrative nests of poorly differentiated nonkeratinizing squamous cell carcinoma (Figure 1).
Osteoclastic giant cells with bland nuclei and lack of atypia. There is also an accompanying lymphocytic infiltrate. H&E stain, x400.
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fig2
Interspersed in between these tumor cells were numerous osteoclastic giant cells with abundant eosinophilic cytoplasm devoid of nuclear atypia, hyperchromatism, or mitotic activity; also there was an accompanying lymphocytic infiltrate (Figure 2).
(a) Reactivity to cytokeratin in the squamous component, lack of reactivity in osteoclastic giant cells. Immunohistochemical stain with anti-CK antibody, x50. (b) Reactivity to P63 in the squamous component, lack of reactivity in osteoclastic giant cells. Immunohistochemical stain with anti-P63 antibody, x50.
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fig3
CD68, CD20, and CD3. CK and P63 were strongly positive in the squamous component and negative in the osteoclastic giant cells (Figure 3), while Vimentin and CD-68 were strongly positive in the giant cell population and negative in the squamous component (Figure 4).
(a) Reactivity to CD68 in the giant cell population and lack of reactivity in the squamous component. Immunohistochemical stain with anti-CD68 antibody, x50. (b) Reactivity to Vimentin in the giant cell population and lack of reactivity in the squamous component. Immunohistochemical stain with anti-Vimentin antibody, x50.
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fig4
CD68, CD20, and CD3. CK and P63 were strongly positive in the squamous component and negative in the osteoclastic giant cells (Figure 3), while Vimentin and CD-68 were strongly positive in the giant cell population and negative in the squamous component (Figure 4).
Reactivity to CD20 (a) and CD3 (b) showed a reactive pattern of the lymphocytic infiltrate. Immunohistochemical stain with anti-CD20 (a) and anti-CD3 (b) antibodies, x100.
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fig5
Expression of CD20 and CD3 showed a reactive pattern of the lymphocytic infiltrate (Figure 5).