PMC3460712_01
(A) Diffuse papillomatous lesion in bulbar conjunctiva covering part of the cornea of patient 1 and (B) In the papillomatous lesion of patient 1, repeating fibrovascular cores with geometrically arranged set of red dots are observed.
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A 48-year-old man with redness in the right eye for 3 years and gradual visual impairment the last year (visual acuity was 0.2 in the right eye) presented a diffuse papillomatous lesion in bulbar conjunctiva covering part of the cornea (Figure 1A and B).
(A) Benign squamous papilloma of patient 1. The papillary growth pattern is apparent. Extensive hyperplastic epithelium with papillomatosis and acanthosis is observed (hematoxylin and eosin staining, x40) and (B) Koilocytosis (arrows) with mild nuclear atypia is observed in the benign squamous papilloma of patient 1 (hematoxylin and eosin staining, x400).
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Extensive hyperplastic epithelium with papillomatosis, acanthosis, and koilocytosis with mild nuclear atypia were observed (Figure 2A and B).
In situ hybridization of the benign squamous papilloma of patient 1. The nuclei of many epithelial cells are positive (arrows) for HPV DNA with broad spectrum probes (x400). . Abbreviation: HPV, human papillomavirus.
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Analysis with DNA in situ hybridization was positive for HPV DNA using low risk probe 6/11 (Figure 3).
PMC3460712_02
Polypoidal papillomatous lesion on the caruncle of patient 2.
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A 24-year-old man presented a polypoidal papillomatous lesion on the conjunctival caruncle of the right eye without any particular symptoms (Figure 4).
(A) Squamous papilloma of patient 2. The papillary growth pattern (arrows) is apparent (hematoxylin and eosin staining, x40) and (B) Koilocytosis (yellow arrow) and mild dysplasia (white arrows) are apparent in the squamous papilloma of patient 2 (hematoxylin and eosin staining, x400).
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A characteristic papillary growth pattern was noted as well as the presence of koilocytosis (Figure 5A and B).
(A) Immunohistochemical analysis of the squamous papilloma with mild dysplasia of patient 2. The nuclei of some epithelial cells show positive staining (arrows) with antibodies to HPV proteins (x400) and (B) Immunohistochemical analysis of the squamous papilloma with mild dysplasia of patient 2. Only a few dysplastic epithelial cells were positive nuclear and cytoplasmic staining (arrows) for p16 protein (x400). . Abbreviation: HPV, human papillomavirus.
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HPV proteins. However, only a few dysplastic epithelial cells showed positive nuclear and cytoplasmic staining for p16 protein (Figure 6A and B).
In situ hybridization of the squamous papilloma with mild dysplasia of patient 2. The nuclei of some epithelial cells are positive (arrows) for HPV DNA with broad spectrum probes (x400). . Abbreviation: HPV, human papillomavirus.
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Analysis with DNA in situ hybridization was positive for HPV DNA using LR probe 6/11 (Figure 7).