PMC3952390_01
The antibiotic susceptibility plate showing no zone of inhibition around the linezolid E-test strip along with the susceptibility pattern for other antibiotics by Kirby Bauer disc diffusion method for the linezolid resistant enterococcus isolate
AJM-4-13-g001.jpg
PMC3952390_01_AJM-4-13-g001.jpg
F1
The antibiotic susceptibility of the isolate was performed by Kirby Bauer disc diffusion technique as per Clinical and Laboratory Standards Institute (CLSI guidelines), which showed the organism to be sensitive to vancomycin, but more remarkably the isolate was resistant to linezolid showing no zone around the disc by the disc diffusion technique [Figure 1]. E-test linezolid strips with a concentration gradient corresponding to 0.016-256 mug/mL were utilized with Mueller-Hinton agar as described by the manufacturer (Hi Media laboratories Mumbai) to determine the MIC of theisolate, but the isolate did not show any zone of inhibition, indicating that the MIC of the isolate was more than 256 mug/mL [Figure 1].