PMC5962088_01
Kinetic cytopathic effect of Zika virus isolated from urine in Vero and SIRC cells detected by qPCR
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- Titration of Zika virus (10-1 to 10-8) isolated in Vero Cells and in the SIRC cells, showing the Ct value of each dilution used for the TCID50 calculation
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Moreover, samples were tested in the qPCR and Ct values (Threshold cycle) are shown in Figure 2.
- Bayesian phylogenetic tree of envelope gene sequences from Brazilian ZIKV using GTR+G+I model with the two Brazilian ZIKV strains obtained in this study MF048807 and MF048808, compared to 16 reference ZIKV sequences from GenBank. Bootstrap values are indicated at the nodes
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The sequences were aligned using Bioedt v.6 and no nucleotide/ amino acid difference were observed among the sequences (Figure 3).
A) SIRC cells uninfected; B) Cytopathic effect in SIRC cells infected with ZIKV after seven days post inoculation with a urine sample; C) Negative staining of ZIKV particle inoculated in SIRC cells observed by Transmission Electron Microscopy; D) Ultrathin section of SIRC cells showing numerous ZIKV particles near endoplasmic reticulum
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The Figure 4A and B show SIRC cells uninfected and infected with ZIKV respectively. Virus particle or "virion" of 50nm in diameter coming from supernatant sample of SIRC cells infected with ZIKV was observed by electron microscopy (Figure 4C). Ultrathin sections of infected SIRC cells presented virus particles with an outer envelope and inner dense core, structures similar in size and morphology of flaviviruses near endoplasmic reticulum and Golgi complex (Figure 4D).