PMC3575407_01
Pedigree of four generation family with EA2.. Generations are shown as I to IV. Individuals affected with episodic ataxia are indicated by a black filled circle or square for females or males. Numbers (1-12) were assigned to individuals who agreed to take blood samples. The proband (III-7) is indicated by an array. DNA samples went to next generation sequencing were labeled with Asterisk (*).
pone.0056362.g001.jpg
PMC3575407_01_pone.0056362.g001.jpg
pone-0056362-g001
SLC1A3, responsible for four EAs, were sequenced by next generation sequencing that was performed by BGI (Shenzhen, China) on subject 3, 6 and 7 (Figure 1a). Experimental procedures included the following three steps. Step 1: library preparation.