PMC3883063_01
Bone marrow aspiration revealed morphological findings compatible with ALL-L2 (May-Giemsa staining, 1000x).
hr-2013-4-e16-g001.jpg
PMC3883063_01_hr-2013-4-e16-g001.jpg
fig001
We detected differences in the size of the blasts, corresponding to an abnormal lymphocyte-like cell population with a high nuclear/cytoplasmic (N/C) ratio, but without granules in the cytoplasm (Figure 1).
A) G-banded karyotype of the bone marrow cells showing t(4;12)(q21;p11) and t(15;17)(q22;q21). Arrows indicate the derivative chromosomes. B) FISH analysis with PML/RARalpha-specific probes showing two orange (PML) and two green (RARalpha) signals. No PML/RARalpha fusion signal (which should appear yellow) was detected.
hr-2013-4-e16-g002.jpg
PMC3883063_01_hr-2013-4-e16-g002.jpg
fig002
Chromosomal analysis with G-banded karyotype of the bone marrow cells showed 46, XX, t(4;12)(q21;p11), t(15;17)(q22;q12) in all 24 metaphase spreads (Figure 2A). Fluorescence in situ hybridization (FISH) performed on interphase nuclei using an LSI PML/RARalpha dual color, dual fusion translocation probe (Vysis, USA) showed two separate PML and RARalpha signals (Figure 2B) in 1000 interphase nuclei and all metaphases analyzed.