PMC7304266_01
Photographs of the ulcer on the forearm: A) active injury; B) injury after the glucantime treatment.
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A 35-years-old male patient, originally from Russia, attended the Dermatology Service of the General Hospital in Mexico due to two ulcerative skin lesions with a semi-dry appearance, active borders, one of which was in the lower left pre-atrial region and the other in the dorsal region of the left forearm (Figure 1A). The case resolved favorably, the lesions healed adequately and the patient was discharged (Figure 1B).
Molecular identification of Leishmania tropica: A) ALAT. PCR products of parasites isolated from the patient in lines 1 and 2 (3 and 2 microL, respectively), Leishmania mexicana positive control (line 3), Nuclease-free water as the negative control (line 4). L=Ladder 100 pb; B) hsp70-C) ITS1. PCR products of parasites isolated from the patient in lines 1 and 3 (3 and 2 microL, respectively), positive control (line 5), and negative control (line 4); D) Maximum Likelihood phylogenetic trees generated using the Tamura three-parameter distance model with Invariant sites (+I) with partial sequences of the ALAT gene, and E) Hasegawa-Kishino-Yano with Gamma distribution (+G) of the hsp70 gene from several members of the genus Leishmania. Bootstrap values greater than 50 are indicated on the nodes. The sequences generated in this study are indicated with red triangles.
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The isolated parasites were used for the PCR evaluation and a successful amplification of the three genes was achieved (Figures 2A, 2B and 2C). The Maximum Likelihood analysis confirmed that the parasite recovered from the patient corresponded to L. tropica (Figures 2D and 2E).