PMC3425269_01
Clear cell sarcoma: low power revealed a dermal malignancy composed of tumor cell nests and fascicles delineated by fibrous septa. No junctional activity or pagetoid spread of abnormal melanocytes (H and E, x100)
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F1
The epidermis did not show any significant increase in melanocytes within the basal layer, junctional activity or pagetoid spread of tumor cells [Figures 1 and 2].
Clear cell sarcoma: Higher magnification showing spindled to epithelioid cells with pale eosinophilic cytoplasm and vesicular nuclei with prominent nucleoli (H and E, x400)
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F2
The epidermis did not show any significant increase in melanocytes within the basal layer, junctional activity or pagetoid spread of tumor cells [Figures 1 and 2].
Immunohistochemical staining showing (a), Intense cytoplasmic immunopositivity for vimentin (Vimentin x400); (b), Cytoplasmic immunoreactivity for Melan-A (Melan-A x 400); (c): Focal cytoplasmic immunoreactivity for HMB-45 (HMB-45 x400); (d), Immunonegative for S-100 protein (S-100, x400)
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F3
The neoplastic cells stained negative for S-100 protein, pancytokeratin, epithelial membrane antigen, and CD 31 [Figure 3].