PMC5153446_01
Numerous tiny polyps diffusely distributed throughout the small intestine mucosa.
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Mucosa in the rest of the small intestine also indicated polypoid appearance (Fig. 1).
Microscopic appearance of the lymphoid polyp found in mucosa of the small intestine (H&E, x100).
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Histopathological analysis of polypoid intestinal specimens was indicative of reactive follicular lymphoid hyperplasia, showing large lymphoid follicles with prominent germinal centres (Fig. 2).
Primary extranodal B cell non-Hodgkin's lymphoma of the small intestine; the histopathological pattern of the "starry-sky" (H&E, x40).
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Apoptotic bodies and "tingle body" macrophages were scattered here and there, altogether giving the "starry-sky" appearance (Fig. 3).
Immunohistochemistry (anti CD10, x400).. (Immunohistochemical stains were performed on 4-mum formalin-fixed paraffin-embedded sections for following antibodies: CD20 (clone L26, Dako, Denmark), BCL6 (clone PG-B6p, Dako, Denmark), BCL2 (clone 124, Dako, Denmark), CD3 (clone PC3/118A, Dako, Denmark), TdT (clone EP266, Dako, Denmark) and Ki67 (clone 30-9, Ventana, UK) using an autostainer (Dako, Carpinteria, CA). For epitope retrieval Heat-induced Epitope Retrieval pH6.0 (Dako) method was used to optimize immunoreactivity for all antibodies followed by a peroxidase blocking kit and the Labeled Strep Avidin Biotin 2 kit (Dako) detection kit with diaminobenzidine as a chromogen (Dako) for optimal visualization of a reaction. Appropriate negative and positive controls were included. Staining of normal B and T lymphocytes in lymphoid tissue of an Appendix was used as internal positive control for CD20, BCL6, BCL2, CD10, CD3 and TdT).
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CD20+, bcl6+ (moderate), bcl2-, CD10+, CD43-, TDT-, CD3-, CK AE1/3-, chromogranin-. CD10 indicated strong positivity (Fig. 4).
Immunohistochemistry (anti Ki67, x400).
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Proliferative activity, measured by Ki67 expression, was found in almost all cells (Fig. 5).