PMC7550620_01
Chest computed tomography scan. Bronchiectasis and multiple granular shadows are randomly distributed in the bilateral lobes, and consolidation in the lower left lobe is present.
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His chest computed tomography (CT) showed bronchiectasis and multiple granular shadows randomly distributed in the bilateral lower and middle lobes, thin-walled cysts predominantly in both upper lobes, and consolidation in the lower left lobe ( Figure 1 ).
Flow cytometric analysis of phosphorylationed STAT1 (pSTAT1) in monocytes after the stimulation with interferon-gamma (500 U/mL). The analysis gate was set in CD14+ cells. Blue areas indicate healthy adults (n = 3), whereas pink areas indicate the patient. Gray and purple areas indicate pSTAT1 in monocytes without interferon-gamma stimulation in healthy adults and the patient, respectively.
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The monocytes of the patient showed hyper-phosphorylation of STAT1 after stimulation with interferon-gamma ( Figure 2 ).
Flow cytometric analysis of B-cell differentiation in the bone marrow (BM). VpreB is a component of the surrogate light chains. The analysis gate was set in CD19+ and/or VpreB+ cells. The BM cells were fixed and permeabilized to simultaneously analyze the cell surface antigens (IgM and CD19) and the intracellular molecule (VpreB).
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The B-cell differentiation was blocked in the stages between VpreB+IgMlow (pre-B1b) cells and VpreB-IgMlow (pre-B2) cells in the bone marrow sample obtained three months after the onset of cryptococcosis ( Figure 3 ).