PMC5847965_01
Local Coprinopsis cinerea skin and soft tissue infection in immunocompromised paediatric patient. Fungus was detected in infected laceration on head (A). Grocott-stained tissue obtained after surgical debridement showed hyphae within and around hair follicles and surrounding inflammatory infiltrate (B). Colonies of C. cinerea grew on Kimmig agar under aerobic conditions (C). Lactophenol cotton blue preparation of fungal colonies displayed hyaline septated hyphae and arthroconidia (D).
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A 6-year-old girl in aplasia, undergoing chemotherapy because of a nephroblastoma with lung metastasis, presented with fever and a 5-cm-long infected laceration on the head (Fig. 1A) which had been sutured one day before at another hospital. White/cream-coloured colonies with aerial mycelium grew on Kimmig agar containing chloramphenicol and oxytetracycline after 48 hours of incubation at 37 C (Fig. 1C). Fungal structures were also observed in lactophenol cotton blue preparations of the culture (Fig. 1B, D).
Phylogenetic tree of Coprinopsis sp. Neighbour-joining-tree was constructed using ITS1 sequences of isolates from this study; most closely related species within genus Coprinopsis. Species and accession numbers (in brackets) are reported for each taxon. Bootstrap values (bootstrap test with 500 replicates) are shown next to branches.
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GU327634.1; Fig. 2).