PMC4150115_01
Immunoblotting and PCR assays. (A): Immunoreactivity of serum patient against H and M fractions of H. capsulatum. (B): Lane M. DNA molecular weight marker 1kb DNA ladder; Lane 1. patient serum collected in February 2008; Lane 2.- patient serum collected in June 2008; Lanes 3 to 10. negative individual sera samples, Lane Hc1. H. capsulatum strain (immunodeficiency); Lane Hc2. H. capsulatum 200 strain; Lane Pb 18. P. brasiliensis strain; Lane Pb 339 P. brasiliensis 339, Lane CN1 e CN2. C. neoformans strains (ATCC 24067) and Lane NO. negative control.
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F1
The reaction of nested PCR was positive for H. capsulatum and C. neoformans, and negative for P. brasiliensis, showing the possible infection by H. capsulatum observed in immunoblotting (Figure 1A). The positive result for Cryptococcus spp., in which fungal DNA was detected in serum and blood samples suggested a concomitant infection, principally by epidemiological similarity (Figure 1B). Figure 1 displays the PCR results from the nucleic acids extracted from the blood and serum.