PMC5634621_01
The neoplastic lymphoid follicles ((a), H&E stain, 400x) are of B-cell lineage ((b), CD20 immunostain, 400x), are admixed with a subset of CD3 positive T-cells ((c), CD3 immunostain, 400x), coexpress CD10 ((d), CD10 immunostain, 400x) and bcl2 positivity ((e), bcl2 immunostain, 400x), and have proliferation rates of approximately 20-30% ((f), MIB-1 immunostain, 400x).
CRIHEM2017-9549428.001.jpg
PMC5634621_01_CRIHEM2017-9549428.001.jpg
fig1
By immunohistochemistry, there are neoplastic B-lymphoid follicles coexpressing CD20+, CD10+, bcl6+, and bcl2+ (Figure 1) and exhibiting proliferation rates of approximately 20-30% (MIB-1 antibody), centred by well-developed CD21+ follicular dendritic meshworks.
The upper dermis ((a), H&E stain, 400x) has large transformed Hodgkin and Reed-Sternberg like cells ((b), H&E stain, immersion oil 1000x) that coexpress CD45 ((c), CD45 immunostain, 400x), CD30 ((d), CD30 immunostain, 400x), and bcl2 positivity ((e), bcl2 immunostain, 400x) and are also PAX5 positive ((f), PAX5 immunostain, 400x).
CRIHEM2017-9549428.002.jpg
PMC5634621_01_CRIHEM2017-9549428.002.jpg
fig2
T-cell population. The H/RS-like cells are largely CD45+ and coexpress CD30, CD20, PAX5, bcl2 (Figure 2), CD79a, bcl6, and MUM1 positivity being negative for CD15.