PMC5857370_01
Cell morphology, Gram staining and colony morphology of isolated bacterium. a) Gram-stain-negative rods from blood culture bottle were observed (magnification x1000). b) White and mucoid colonies on sheep blood agar after culture for 48 h at 35  C.
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F1
Of the four blood culture vials, two aerobic culture vials showed a positive signal 40 h later, and Gram staining of the blood culture fluid samples showed Gram-negative rods (Fig. 1a). Identical oxidase-positive, urease-positive, non-lactose-fermenting colonies were isolated from both blood culture vials the next day (Fig. 1b).
Cladogram phylogenetic tree of the 16S rRNA gene sequences of strain Naga 0113 =PAGU 1967; the tree was prepared by the neighbour-joining method. Numbers at nodes are bootstrap values, expressed as a percentage of 1000 replications. The scale bar represents one inferred nucleotide substitution per 100 nucleotides.
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F2
According to phylogenetic trees created using neighbour-joining method (Fig. 2), this strain (Naga 0113=PAGU 1967) was most closely related to the R. pusense genotype, among all species of the genus Rhizobium.