PMC2797364_01
PMC2797364_02
Computed Tomography of the C Spine, with 3D reconstruction, showing Klippel-Feil anomaly comprising of the fusion of multiple cervical and upper thoracic vertebrae.
CRM2009-361518.001.jpg
PMC2797364_02_CRM2009-361518.001.jpg
fig1
PMC2797364_03
(a) Nucleotide sequence chromatograms across the COMT G675A haplotype using (i) patient DNA, (ii) patient cDNA, (iii) mRNA/cDNA from a normal control heterozygote. (b) Expression of the COMT G675A variants. COMT amplicons from semiquantitative RT-PCR were visualized on an agarose gel after 33 cycles using beta
2-actin and GAPDH (housekeeping genes) as the quantitation control. Lane 1:no cDNA control, lane 2:patient, lane 3 & 4:normal control individuals that were homozygous for the 675G allele.
CRM2009-361518.002.jpg
PMC2797364_04_CRM2009-361518.002.jpg
fig2
Only the patient (Figure 2(b), lane 2) expressed the 675A allele and both the patient and two normal controls (lanes 3 and 4) had comparable levels of expression of the 675G allele, as evident from the agarose gel electrophoresis of the amplicons.
PMC2797364_04
(a) Nucleotide sequence chromatograms across the COMT G675A haplotype using (i) patient DNA, (ii) patient cDNA, (iii) mRNA/cDNA from a normal control heterozygote. (b) Expression of the COMT G675A variants. COMT amplicons from semiquantitative RT-PCR were visualized on an agarose gel after 33 cycles using beta
2-actin and GAPDH (housekeeping genes) as the quantitation control. Lane 1:no cDNA control, lane 2:patient, lane 3 & 4:normal control individuals that were homozygous for the 675G allele.
CRM2009-361518.002.jpg
PMC2797364_04_CRM2009-361518.002.jpg
fig2
Only the patient (Figure 2(b), lane 2) expressed the 675A allele and both the patient and two normal controls (lanes 3 and 4) had comparable levels of expression of the 675G allele, as evident from the agarose gel electrophoresis of the amplicons.