PMC7723486_01
Individual data from Patient 10, who had HCV recurrence after liver transplantation. Liver biopsy (left panels) was performed 3 months after transplantation. (a) Hematoxylin-eosin staining revealed typical signs of HCV recurrence. (b and detail in c) Immunostaining for the M30 neoepitope revealed positivity in apoptotic hepatocytes throughout the liver lobule. (d) Immunostaining for K19 revealed only the normal positivity in bile ducts. Time-course changes in standard biochemical markers (AST, ALT, GGT, and alkaline phosphatase (AP)) and serum keratins (TPS, M30, and CYFRA 21-1) are presented in the right panels. d, day; mo, month.
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fig4
Individual data for one of the patients with HCV recurrence are presented in Figure 4. The increase in serum keratin levels, particularly for K19, tended to occur earlier than alterations in standard liver markers (Figure 4). Liver biopsy was consistent with HCV recurrence (Figure 4). Immunohistochemical staining for K19 revealed that the expression of this protein was almost entirely restricted to cholangiocytes (Figure 4).
Individual data from Patient 4, who had acute cellular rejection after liver transplantation. Liver biopsy (left panels) was performed on day 33 after transplantation. (a) Hematoxylin-eosin stain revealed typical signs of rejection. (b) Immunostaining for the M30 neoepitope was negative. (c) Immunostaining for K19 revealed the normal positivity in the bile ducts and in a few scattered hepatocytes. Time-course changes in standard biochemical markers (AST, ALT, GGT, and alkaline phosphatase (AP)) and serum keratins (TPS, M30, and CYFRA 21-1) are presented in the right panels. d, day; mo, month.
CRIHEP2020-8895435.005.jpg
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fig5
Individual data for one of the patients with acute rejection are presented in Figure 5. Conversely, we observed a transient increase in serum K19 fragment (CYFRA 21-1) (Figure 5). Immunohistochemical staining for K19 revealed that it was almost entirely restricted to cholangiocytes (Figure 5).