PMC2835670_01
Phylogenetic tree based on the E gene sequences using Neighbor-joining (NJ) method showing the relationship of viruses isolated in this study with 72 global samples of DENV. Tamura and Nei (TrN+I+G) nucleotide substitution model was used with a proportion of invariable sites (I) of 0.2096 and gamma distribution (G) of 0.6072 using the hierarchical likelihood ratio test (hLTR). The YF17D (9627244) was used as outgroup. Horizontal branch lengths are drawn to scale. Viruses isolated from patients 1 and 2 are underlined. GenBank accession numbers are indicated in parenthesis.
1743-422X-7-22-1.jpg
PMC2835670_01_1743-422X-7-22-1.jpg
F1
This analysis showed that patient 1 was infected with dengue virus type 2 and patient 2 with dengue virus type 3 (Figure 1).