PMC5977435_01
Agarose gel electrophoresis (2.2%) of 18S gene PCR amplification from cultures of Pentatrichomonas hominis. Lanes 1, 3 and 4: suspected negative samples (from others cats studied by our group, and not described in this paper). Lane 2: positive sample from the male cat. Lane 5: positive sample from the female cat. Lane 6: negative control. Lane 7: 50 bp DNA ladder (Promega)
10.1177_2055116918774959-fig1.jpg
PMC5977435_01_10.1177_2055116918774959-fig1.jpg
fig1-2055116918774959
There was only amplification of the samples in the test for P hominis (Figure 1), and the corresponding amplicon were purified with Kit Wizard SV Gel and PCR Clean-Up System (Promega), following the manufacturer's protocols.