PMC10382241_01
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) results of CYP2C9*3 and CYP2C8*3 polymorphisms. (a) Agarose gel electrophoresis analysis for CYP2C9*3 polymorphism following digestion with Mph1103I (NsiI). Lane 1: 100 bp DNA marker. Lane 2: undigested PCR product (168 bp). Lane 3: digestion of the homozygous mutant CC genotype yields two bands (50 and 118 bp). Since the molecular weight of 50 bp is very small, it could not be distinguished on the gel. The major visible band was of 118 bp. (b) Agarose gel electrophoresis analysis for CYP2C8*3 polymorphism following digestion with PdmI (XmnI). Lane 1: 50 bp DNA marker. Lane 2: undigested PCR product (117 bp). Lane 3: digestion of the homozygous mutant GG genotype is not cut and yields one band (117 bp).
CRIM2023-6623269.001.jpg
PMC10382241_01_CRIM2023-6623269.001.jpg
fig1
The representative agarose gel images of CYP2C9*3 and CYP2C8*2 PCR-RFLP assays were used to interpret the patient's genotypes (Figure 1). The CYP2C9*3 allele produced 50 bp and 118 bp after digestion with Mph1103I (NsiI) (Figure 1(a)), while the CYP2C8*3 allele produced as a single band at 117 bp that is not cut following the digestion with PdmI (XmnI) (Figure 1(b)).