PMC4880429_01
Photomicrography of the lymph node showing typical histology and immunophenotype of a chronic lymphoid leukemia diagnosis. A - Lymph node obliterated by small and mature lymphoid cells (H&E, 100X); B - CD20 membranous positivity (IH-Cell Marque  L26 clone, 200X); C - CD23 positivity (IH-Cell Marque  1B12, 200X); D - CD5 aberrant positivity (IH-Cell Marque  4C7 clone, 200X); E - Ki67 low index positivity (IH-Cell Marque  SP6 clone, 200X).
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The lymph node histology showed complete replacement of the lymph node architecture by small lymphoid cells (Figure 1A). The immunohistochemistry was positive for CD-20 (Cell Marque  L26), CD-23 (Cell Marque  1B12), and CD-5 (Cell Marque  4C7); and negative for CD-3 (Cell Marque  MRQ-39), cyclin-D1 (Spring  SP4), and CD-10 (Cell Marque  56C6) (Figure 1B, D). The Ki-67 (Spring  SP6) showed a low index of positivity (Figure 1E).
Photomicrography of the lymph node showing partial cytoplasmic positivity for ZAP-70 of the neoplastic cells with moderate or strong intensity (Immunohistochemistry-Ventana  2F3.2, 600X).
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Zeta-chain-associated protein kinase-70 (ZAP-70) (Ventana  2F3.2) was positive (Figure 2) and p53 (Cell Marque  DO7) was negative.