PMC3988652_01
Radiography showing a radiolucent area with bone resorption between teeth 22 and 26
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F1
The radiographic examination showed the presence of a unilocular radiolucent area between the left superior lateral incisor and the first left molar [Figure 1].
Lesion surgical enucleation and extraction of tooth 26
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F2
An incisional biopsy was performed; removing all the granulomatous tissue present in the area and tooth 25 was removed, as it was decayed [Figure 2].
Specimen for histopathological analysis tooth 26
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F3
The specimen was sent for histopathological analysis [Figure 3].
(a) Histological features of the biopsy specimen showing cellular connective tissue and presence of multiple islands and strands of odontogenic epithelium (Hematoxylin-eosin stain; original magnification x400). (b) Immunohistochemical reaction for AE1/AE3 : positive staining for odontogenic epithelium (original magnification x400)
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F4
The microscopic examination revealed a cellular connective tissue with multiple islands and strands of odontogenic epithelium covered by stratified squamous epithelium [Figure 4a]. Immunohistochemical reactions for AE1/AE3 showed positive staining for an odontogenic epithelium, confirming the central odontogenic fibroma WHO-type diagnosis [Figure 4b].