PMC9035922_01
Detection of CMV infection by IHC. A stomach tissue section stained with a monoclonal antibody directed against CMV pp65 antigen is shown. Arrows indicate IHC-positive cells. IHC staining using a monoclonal antibody against CMV pp65 antigen (>2 cells stained; magnification, x1,000). IHC, immunohistochemistry.
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F1
Immunohistochemistry staining using a monoclonal antibody against CMV pp65 antigen indicated CMV immunoreactivity with brownish areas (shown in Fig. 1).
Agarose gel electrophoresis of the PCR products obtained from FFPE tissues using the specific primers amplified a 177-bp fragment within the coding region of gB. Lane 1: non-template control, lane 2 and 3: 177-bp CMV DNA fragment in the gall bladder, lanes 4-11: 177-bp CMV DNA fragment in gall bladder in perforation site of the gastric wall, lane 12: 177-bp positive control, and lane 13: 100-bp DNA marker.
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F2
A nested-PCR confirmed CMV infection for CMV DNA in gall bladder specimens and gastric wall biopsy (shown in Fig. 2).