PMC3147088_01
(a) Showing verrucous lesion; (b) sclerotic bodies in KOH preparation (x40); (c) Sabouraud's dextrose agar slant with small, smooth, folded olive black colonies and (d) lactophenol cotton blue preparation of the colony with brown septate hyphae and lateral and terminal conidiophores
JLP-2-47-g001.jpg
PMC3147088_01_JLP-2-47-g001.jpg
F1
Eighty-one year old male patient, a priest by occupation and residing in a rural area, presented with large verrucous lesions over the lateral aspect of right leg since 6 years [Figure 1a] and smaller plaque of 1 year duration on the lower left leg. On local examination, there was a verrucous lesion measuring 15 x 10 cm with crusting, discharge and "satellite lesions" [Figure 1a]. Direct microscopic examination of 10% KOH preparation of the scrapings and crusts from the lesions showed small, round, thick-walled, brownish septate sclerotic bodies measuring 6-12 mum [Figure 1b]. The specimen was inoculated on Sabouraud's dextrose agar slant containing chloramphenicol and yielded small, smooth, folded olive black colonies after 2 weeks incubation at room temperature [Figure 1c]. Lactophenol cotton blue preparation of the colony revealed brown septate hyphae with lateral and terminal conidiophores of varying size, bearing ovoid, dark conidia in branching chain formation (Cladosporium type of sporulation) [Figure 1d].