PMC8453197_01
Excised thyroglossal duct cyst specimen from a 4-year-old female patient (case 1). (A) Paired iodinated micro-CT imaging at 17.3 micron resolution and (B) histopathological section with H&E staining in sagittal section demonstrate the thick-walled thyroglossal duct cyst (*) and the thyroglossal duct (dashed arrows) anterior to the hyoid bone (B) and hyoid cartilage (C).
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F1
Direct radio-pathological comparisons between the micro-CT imaging and histopathological specimen appearances are provided in Figures 1-5, Supplementary Video 1. Micro CT images more easily than the histopathology sections, and in combination they provided an excellent guide to interpreting complete surgical excision in these cases. Micro-CT also allowed for a 3D imaging "stack" to be stored in a digital format, which was particularly helpful for one of the cases (Figure 3, Supplementary Video 1) where the thyroglossal duct was not included in the pathological section but could be clearly delineated by reconstructing the micro-CT images.
Excised thyroglossal duct cyst specimen from a 5-year-old male patient (case 2). (A) Paired iodinated micro-CT imaging at 20.0 micron resolution and (B) histopathological section with H&E staining in sagittal section demonstrate a large bilobed thyroglossal duct cyst (*) with mixed internal contents containing blood and pus, leading to a blind ending thyroglossal duct (dashed arrows) anterior to the hyoid bone (B) and hyoid cartilage (C).
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F2
Direct radio-pathological comparisons between the micro-CT imaging and histopathological specimen appearances are provided in Figures 1-5, Supplementary Video 1.
Excised thyroglossal duct cyst specimen from a 6-year-old male patient (case 3). (A) Paired iodinated micro-CT imaging at 19.9 micron resolution, and (B) histopathological section with H&E staining in sagittal section demonstrate a well encapsulated thyroglossal duct cyst (*), just anterior to the hyoid cartilage (C). The hyoid bone is demonstrated by the "B." Although the thyroglossal duct was not present in the histopathological section, it was possible from the 3D imaging dataset to reconstruct the (C) sagittal viewing plane for better duct visualisation (dashed arrow).
fped-09-746010-g0003.jpg
PMC8453197_01_fped-09-746010-g0003.jpg
F3
Direct radio-pathological comparisons between the micro-CT imaging and histopathological specimen appearances are provided in Figures 1-5, Supplementary Video 1. Micro CT images more easily than the histopathology sections, and in combination they provided an excellent guide to interpreting complete surgical excision in these cases. Micro-CT also allowed for a 3D imaging "stack" to be stored in a digital format, which was particularly helpful for one of the cases (Figure 3, Supplementary Video 1) where the thyroglossal duct was not included in the pathological section but could be clearly delineated by reconstructing the micro-CT images.
Excised thyroglossal duct cyst specimen from a 16-month old female patient (case 4). (A) Paired iodinated micro-CT imaging at 14.2 micron resolution and (B) histopathological section with H&E staining demonstrate the thyroglossal duct (dashed arrows) anterior to the hyoid bone (B) and hyoid cartilage (C). An artefactual cleft is demonstrated with the open arrow. There is no cystic structure on this image. Some skin (S) and fatty tissue (F) from the neck was removed at surgery, and areas of chronic inflammation with tethering of the adjacent musculature and surrounding tissues (solid arrows) are shown.
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F4
Direct radio-pathological comparisons between the micro-CT imaging and histopathological specimen appearances are provided in Figures 1-5, Supplementary Video 1.
Excised thyroglossal duct cyst specimen from a 14-year old female patient (case 5). (a) Paired iodinated micro-CT imaging at 22.2 micron resolution and (b) histopathological section with H&E staining demonstrate the thyroglossal duct (dashed arrows) anterior to the hyoid bone (B) with areas of cystic change (remnants) from a previously infected and ruptured cyst (*).
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PMC8453197_01_fped-09-746010-g0005.jpg
F5
Direct radio-pathological comparisons between the micro-CT imaging and histopathological specimen appearances are provided in Figures 1-5, Supplementary Video 1.