PMC7178951_01
a (Hematoxylin and Eosin, x10): in this picture an important linfoplasmacellular ad granulocytic infiltrate is appreciable in the lamina propria with a predominance of eosinophilis, with an infiltrating pattern sometimes disrupting glandular integrity. b (Hematoxylin and Eosin, x20): a particular of the previous where eosinophils granulocytes surround and infiltrate the glands. As a consequence, glandular structures are depleted of their goblet cells and show reactive hyperchromatic nuclei. c (Hematoxylin and Eosin, x40): this is an atrophic field where the linfomonocytic and eosinophil granulocitic infiltrate disrupt the glandular structures evocating atrophy and reactive changes. d (Hematoxylin and Eosin, x10): this is a picture of the same patient after therapy. You can appreciate the lamina propria devoid of inflammatory infiltrate. The glands are well separated, normoconformed and with a goblet component normorapresented. Just in a focal small field there's a linfomonocytic infiltrate, where no one can appreciate eosinophilic component. e (Hematoxylin and Eosin, x20): a particular of the previous, where glands are normal and neither a significant eosinophilic infiltrate, nor reactive hyperchromatic changes, nor mucin depletion can be appreciated. f (Hematoxylin and Eosin, x20): as the previous one, glandular mucosal component is conserved and just a focal eosinophilic infiltrate in three different glands is present, but without any specific feature
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Fig1
Biopsies collected from apparently normal mucosa showed instead an important linfoplasmacellular and granulocytic infiltrate in the lamina propria with a predominance of eosinophils (Fig. 1a, b).
a, b Flow cytometric analysis of basophils activation. Whole blood was used immediately after collection from the patients. Stimulation of basophils was performed on whole blood according to the instructions of the supplier (Beckman Coulter, France), using the following monoclonal antibodies: CD3-PC7 (clone UCHT1, Beckman Coulter), CRTH2-FITC (clone BM16, Beckman Coulter), CD203c-PE (clone 97A6, Beckman Coulter), CD63-PC5 (clone H5C6, Beckton Dickinson) and CD125-APC (clone 26815, R&D Systems) and the Navios Flow Cytometer (Beckman Coulter). All data generated were analyzed using Kaluza software (Beckman Coulter). c Serum levels of IL5 (ELISA; R&D Systems; sensitivity: 0.29 pg/ml) at baseline and after 3, 6 and 12 months of treatment were tested
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Fig2
Results are shown in Fig. 2a, b. Systems; sensitivity: 0.29 pg/ml) were measured at baseline and after 3, 6 and 12 months of treatment and are shown in Fig. 2. Serum IL-5 was non-quantifiable at baseline and showed an increase with peak on 6 months as expected and previously demonstrated by Pouliquen et al. in 2015 (Fig. 2c).