PMC8203365_01
Array CGH, conventional cytogenetic, and FISH analyses with a FLT3/CON13 probe. Karyotype (a) and metaphase spread (b) showing 46,XY,i(17)(q10),del(20)(q11.2q13.3) 1~20dmin. (c) Array comparative genomic hybridization (CGH) was performed following the standard protocol provided by Agilent Technologies (Agilent Technologies, Santa Clara, CA, United States) showing the region of amplification. Equal quantities of labeled DNA products were mixed together and loaded onto Agilent'3 2*400 k CGH chip, which is built based on GRCh37/hg19 with 1 kb median probe spacing. (d) FLT3/CON13 FISH probe. Interphase FISH (e) and metaphase FISH (f) showing numerous copies of FLT3.
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fig1
The karyotype result was designated as 46,XY,i(17)(q10),del(20)(q11.2q13.3)[8]/46,idem,1~20dmin[12] (Figures 1(a) and 1(b)). The amplified region includes POLR1D, FLT3, RPL21, PDX1, and CDK8 (Figure 1(c)). Probe design is shown in Figure 1(d). FISH result was nuc ish (FLT3 amp, CON13x2) [136/200] (Figures 1(e) and 1(f)). FISH results confirmed the presence of FLT3 amplification in this patient.