PMC8101672_01
On addition of 1 ml of Ehrlich reagent (4-dimethylaminobenzaldehyde) to 1 ml of urine produces port wine color in patient's urine containing PBG
IJN-31-67-g001.jpg
PMC8101672_01_IJN-31-67-g001.jpg
F1
Chloroform is added to the test tubes, mixed well and allowed to settle. Two immiscible layers are formed; with chloroform being heavier, sinks to the bottom and urine being aqueous, stays on top with red color
IJN-31-67-g002.jpg
PMC8101672_01_IJN-31-67-g002.jpg
F2