PMC9742836_01
PMC9742836_02
PMC9742836_03
Experimental overview. The goal of the study was to evaluate gene expression profiles using TempO-Seq on aged formalin-fixed paraffin-embedded (FFPE) samples compared to paired frozen (FROZ) samples (n = 6/dose level/preservation). Previous RNA-Sequencing (RNA-Seq) profiles were used as referent data (n = 6/dose level/preservation). Samples consisted of paired liver tissue from male mice exposed to multiple dose levels of dichloroacetic acid (DCA) by drinking water for 6 days that were either flash frozen or formalin-fixed in 10% neutral buffered formalin (NBF) for 24 h. followed by paraffin-embedding. Samples were then subjected to RNA-Seq and TempO-Seq > 20 years later.
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TempO-Seq platform and to compare these profiles with those obtained using Illumina whole-genome RNA-Seq technology. To do this, we identified differentially expressed genes (DEGs) induced by a well-studied reference chemical (dichloroacetic acid, DCA) in paired FFPE and flash-frozen (FROZ) samples using TempO-Seq and then compared those results to previous total RNA-Seq data generated from the same paired samples (Fig. 1).