PMC4548749_01
The tumor was composed of a monotonous spindle-cell proliferation.. Notes: Hematoxylin and eosin stain; original magnification: 10x.
ott-8-1997Fig1.jpg
PMC4548749_01_ott-8-1997Fig1.jpg
f1-ott-8-1997
Histologically, the tumor was composed of bland spindle cells (Figures 1 and 2A).
Gastrointestinal stromal tumor cell histology.. Notes: (A) Tumor cells were cytologically bland. No areas of necrosis or atypical mitoses were detected (hematoxylin and eosin stain; original magnification: 10x). (B) Tumor cells expressed strong and diffuse positivity for CD117 (KIT) (original magnification: 20x). (C) Tumor cells expressed strong and diffuse positivity for DOG-1 (original magnification: 20x).
ott-8-1997Fig2.jpg
PMC4548749_01_ott-8-1997Fig2.jpg
f2-ott-8-1997
Histologically, the tumor was composed of bland spindle cells (Figures 1 and 2A). Immunocytochemical stains revealed strong cytoplasmic expression of CD117, DOG1, and CD34 (Figure 2B and C).
Electropherogram obtained by bidirectional Sanger sequencing of two independent amplifications, both showing a pN822K mutation.
ott-8-1997Fig3.jpg
PMC4548749_01_ott-8-1997Fig3.jpg
f3-ott-8-1997
Terminator chemistry, on a 3500 Dx Genetic Analyzer. The test results showed a single mutation in exon 17 of the c-KIT gene (pN822K; Figure 3), confirmed in two independent amplifications, while the PDGFRalpha mutational status was wild type.