PMC4451678_01
Photomicrograph showing gram positive, nonbranching, septate hyphae (Gram stain, x1000)
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F1
On microscopic examination, numerous Gram positive nonbranching septate hyphae, few epithelial cells and debris were observed [Figure 1].
Photomicrograph showing entangled hyphae and contiguous immature arthroconidia (Sabouraud's dextrose agar with chloramphenicol, x450)
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F2
After incubation at room temperature for 24 h, microscopic examination of the colonies revealed numerous entangled hyphae and immature contiguous spores [Figure 2].
Culture on Sabouraud's medium showing circular, white, flat colonies
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F3
At the end of 48 h, white circular colonies with fine granular or powder-like surface were observed [Figure 3].
Photomicrograph showing gram positive, barrel-shaped arthrospores (Gram stain, x1000)
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F4
Gram stained smear of the material obtained from colonies showed numerous cylindrical or barrel-shaped arthrospores [Figure 4].
Photomicrograph showing rectangular arthrospores with rounded ends (wet unstained smear, x450)
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F5
A wet unstained smear was prepared and examined under microscope, which also showed rectangular arthroconidia [Figure 5].
Urease test: Urease test of control specimen (Trichosporon spp) showing pink color (positive test), while urease test of present case showing negative result
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F6
Trichosporon species. To differentiate these two, urease test was carried out, which was negative [Figure 6].