PMC6757491_01
Case 1: Light microscopy findings. (A) Necrotic and hemorrhagic tissue with abundant pigmented hyphae (hematoxylin-eosin 100x, original magnification). (B) Hyphae with pigmented cytoplasm (hematoxylin-eosin 400x, original magnification). (C) Coenocytic hyphae with hyaline and granular pigmented cytoplasm forms; note the absence of pigmentation of its cell wall (hematoxylin-eosin 400x, original magnification).
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The mycotic wall did not present coloration (Figure 1); in some areas, non-pigmented, coenocytic hyphae predominated.
Case 1: Electron microscopy findings. (A) Necrotic tissue with a coenocytic hyphae (blue arrows) showing a floccular and slightly granular electron-dense cytoplasm (uranyl acetate, lead citrate 6000x, original magnification). (B) Coenocytic hyphae, note the cytoplasm (C), the plasmatic membrane (red arrow), and the multilayered cell wall (CW) with intense and variable electron density (uranyl acetate, lead citrate 43 000x, original magnification).
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They also revealed a multilamellar cell wall with variable electron density, but being mostly highly electron-dense (Figure 2).
PMC6757491_02
Case 2: Light microscopy findings. (A) Thick, regular hyphae, some slightly pigmented (hematoxylin-eosin 400x, original magnification). (B) Regular thick hyphae and pigmented cell wall in longitudinal section (hematoxylin-eosin 100x, original magnification). (C) Thick pigmented cell wall hyphae in cross-section (hematoxylin-eosin 100x, original magnification).
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They revealed a pigmented, yellowish-brown cell wall (Figure 3).
Case 2: Electron microscopy findings. (A) Well-defined hyphae (blue arrows); note its cytoplasm (C), cell wall (CW), and a clear central artifactual band (A) (uranyl acetate, lead citrate 6000x; original magnification). (B) Hyphae CW with trilaminar architecture; the external is thinner and highly electron-dense and corresponds to melanin granules (M) (uranyl acetate, lead citrate 43 000x; original magnification).
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This examination revealed hyphae with regular, trilaminar cell wall in which the most external layer, very thin and granular, was consistent with granular melanin (Figure 4).