PMC8060769_01
Spleen. Fine-needle aspiration cytology. Large lymphoid cells exhibit
pleomorphism and occasionally contain erythrocytes in their cytoplasm.
Wright-Giemsa staining
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On fine-needle aspiration of the spleen and liver, a large population of pleomorphic lymphocytes of intermediate size (10-15 mum diameter) was found (Figure 1).
Spleen. Neoplastic lymphoid cells infiltrate red pulp. Haematoxylin and eosin
staining
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In the spleen, extensive necrosis and haemorrhage with infiltration of neoplastic lymphoid cells were observed (Figure 2).
Liver. Hepatic cords are infiltrated by neoplastic lymphoid cells, and
hepatocytes exhibited degeneration and necrosis. Haematoxylin and eosin
staining
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The neoplastic lymphoid cells diffusely infiltrated the hepatic sinusoids and hepatic cords in the liver (Figure 3).
A neoplastic lymphoid cell in a hepatocyte containing an erythrocyte.
Transmission electron microscopy. Scale bar = 10 microm
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Ultra-structurally, the neoplastic cells were often located in the cytoplasm of degenerated hepatocytes and they often contained one or more erythrocytes (Figure 4).
Liver. Neoplastic lymphoid cells in the cell membrane and cytoplasm are
positive for CD3. Inset: a neoplastic lymphoid cell inside a hepatocyte is
also positive
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Immunohistochemically, the membrane of neoplastic cells was strongly positive for CD3 (Figure 5).
Liver. Neoplastic lymphoid cells in the cytoplasm are positive for granzyme
B
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Granular positivity for granzyme B and T-cell intracellular antigen 1 (TIA-1/GMP-17) were observed in the cytoplasm of neoplastic lymphoid cells (Figures 6 and 7).
Liver. Neoplastic lymphoid cells in the cytoplasm are positive for
TIA-1/GMP-17
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Granular positivity for granzyme B and T-cell intracellular antigen 1 (TIA-1/GMP-17) were observed in the cytoplasm of neoplastic lymphoid cells (Figures 6 and 7).