PMC3204653_01
Macrorestriction analysis (XbaI) by pulsed-field gel electrophoresis of Klebsiella pneumoniae N100469 (lane 1), K. pneumoniae N10-0506 (lane 2), Escherichia coli N10-0505 (lane 3), E. coli N10-0705 (lane 4), Salmonella enterica serovar Branderup molecular mass marker (XbaI) (lanes SB)..
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F1
Macrorestriction analysis of the 4 isolates showed that K. pneumoniae N10-0469 and N10-0506 were closely related, although E. coli N10-0505 and N10-0705 were not (Figure 1).
BglII restriction digests of New Delhi metallo-beta-lactamase (pNDM)-Kp10469 (lane 1) and pNDM-Ec10505 (lane 2). MW, 1-kb extension ladder molecular mass marker (Invitrogen, Carlsbad, CA, USA). Sizes (kb) are indicated for some bands.
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F2
PCR and sequencing showed that CMY-6 and NDM-1 were located on an  102-kb plasmid (pNDM-Kp10469), originating from K. pneumoniae N10-0469, and on an  129-kb plasmid (pNDM-Ec10505), originating from E. coli N10-0505 (Figure 2). Incompatibility (Inc) group PCR and fingerprinting showed that both plasmids were related Inc A/C types, indicating possible horizontal transfer in vivo (Figure 2).