PMC7323278_01
Peripheral blood film shows hypochromic microcytic erythrocytes, polychromasia, spherocytes, numerous target cells, and irregular contracted erythrocytes.
10.1177_1179547620936424-fig1.jpg
PMC7323278_01_10.1177_1179547620936424-fig1.jpg
fig1-1179547620936424
Peripheral blood film (Figure 1) portrayed features consistent with hemoglobinopathy associated with chronic hemolysis.
Capillary electrophoresis shows predominantly Hb A of 69.1%, Hb A2 of 0.4%, Hb F of 0%, Hb H of 26.6%, Hb Barts of 1.5% and Hb Constant Spring of 2.4%.
10.1177_1179547620936424-fig2.jpg
PMC7323278_01_10.1177_1179547620936424-fig2.jpg
fig2-1179547620936424
Capillary electrophoresis (Figure 2) showed Hb A of 69.1%, Hb A2 of 0.4%, Hb F of 0%, Hb H of 26.6%, Hb Barts of 1.5% and Hb CS of 2.4%.
Agarose gel electrophoresis shows a faster-move band (Hb H band) on the (A) alkaline and (B) acid gel electrophoresis.. P: Patient, C: Control.
10.1177_1179547620936424-fig3.jpg
PMC7323278_01_10.1177_1179547620936424-fig3.jpg
fig3-1179547620936424
The agarose alkaline and acid gel electrophoresis (Figure 3A and B) revealed a faster-move band (Hb H band).