PMC3861856_01
Fundus findings before vitrectomy. A fundus photograph shows diffuse vitreous opacity in the right eye. The fundus became cloudy compared to the healthy eye. No obvious opacity can be seen in the left eye.
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F1
His best-corrected visual acuity had decreased to 0.5, and funduscopic examination revealed diffuse vitreous opacity (fig. 1).
Flow cytometry analysis reveals both the vitreous and the peripheral blood. a A high rate (35.4%) of CD4 and CD8 double-positive T cells was detected in the vitreous sample. b A low rate (1.4%) of CD4 and CD8 double-positive T cells was detected in the peripheral blood. c ATL flower cells can be seen in the vitreous sample. A large number of typical ATL cells were detected in the vitreous sample, which had many nuclear lobules and basophilic cytoplasm. d Detection of proviral DNA of HTLV-I in the vitreous of our ATL patient. M = Molecular size marker; N = negative control; P = positive control (HTLV-I-infected cells); S = vitreous sample of our ATL patient. Arrow indicates 300 bp.
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F2
A high level of CD4 and CD8 double-positive T cells was detected in the vitreous fluid but not in the peripheral blood (fig. 2a, b), which is rarely seen in other cases of uveitis. Cytological examination of the vitreous specimen revealed flower cells that infiltrated into the vitreous (fig. 2c). Monoclonal T-cell receptor chain rearrangement was also detected by PCR in the pure vitreous sample (fig. 2d).