PMC8565984_01
Clinical characteristics of Case 1. (A) The structure of tandem CD19/CD22 CAR. (B) Flow cytometry analysis of BM samples before and after CAR T-cell infusion. (C) The infusion schedule of Case 1, with FC conditioning regimen and 3 consecutive days CAR T-cells infusion. (D) The changes in temperature and the WBC count post CAR T-cell infusion. Case 1 suffered high fever at day 3 after infusion. With the increase in the WBC count and supportive treatment, the patient's temperature returned to normal. (E) CAR T-cell copies detected by qPCR post infusion.
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The EP300-ZNF384 fusion gene was also detected with RNA sequencing, with fusion of exon 6 of EP300 to exon 3 of ZNF384 (Supplementary Figure 1B).
PMC8565984_02
Clinical characteristics of Case 1. (A) The structure of tandem CD19/CD22 CAR. (B) Flow cytometry analysis of BM samples before and after CAR T-cell infusion. (C) The infusion schedule of Case 1, with FC conditioning regimen and 3 consecutive days CAR T-cells infusion. (D) The changes in temperature and the WBC count post CAR T-cell infusion. Case 1 suffered high fever at day 3 after infusion. With the increase in the WBC count and supportive treatment, the patient's temperature returned to normal. (E) CAR T-cell copies detected by qPCR post infusion.
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The EP300-ZNF384 fusion gene was also detected with RNA sequencing, with fusion of exon 6 of EP300 to exon 3 of ZNF384 (Supplementary Figure 1B).
Clinical characteristics of Case 2. (A) Flow cytometry analysis of BM samples before and after CAR T-cell infusion. (B) The infusion schedule of case 2. The patient got 4 days CAR T-cells infusion with 2 x 107/kg. (C) The changes in temperature and the WBC count post CAR T-cell infusion. Case 2 suffered high fever at day 4 after infusion. With the increase in the WBC count and supportive treatment, the patient's temperature returned to normal. (D) CAR T-cell copies detected by qPCR post infusion.
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BM morphology showed that 60% of blasts were positive for CD13, CD19, CD22, CD33, CD34, CD38, cCD79a, and HLA-DR and negative for CD20 (Figure 2A). T-cells, which was followed by three infusions administered for the next 3 days. The effective anti-CD19/CD22 CAR T-cells totaled 2 x 107/kg (Figure 2B). She suffered grade 2 CRS and presented with fever and hypotension, which was relieved by symptomatic treatments and low-dose norepinephrine (Figure 2C). Amplification of CAR T-cell as detected by qPCR peaked at day 10 and dropped detectable before HSCT (Figure 2D).