PMC5182032_01
Bone marrow examination showing infiltrate with cells of lymphoid morphology, intact (arrows), or smudged.. Note: No azurophilic granules are observed (bone marrow - May-Grunwald-Giemsa stain, x100 magnification).
ott-10-031Fig1.jpg
PMC5182032_01_ott-10-031Fig1.jpg
f1-ott-10-031
The infiltrate was difficult to quantify because of the presence of >90% smudge cells (Figure 1).
Dot plot histograms showing atypical suppressor CD5-CD3+CD8+ T cells (in red) and normal CD3+CD5+ T-cells (blue).. Notes: FACSCalibur acquisition with CellQuest acquisition software of bone marrow aspirate leukocytes marked with a monoclonal antibody panel including CD3 PC5, CD4 FITC, CD5 FITC, and CD8 PE. Infinicyt 7.0 software was used for analysis.
ott-10-031Fig2.jpg
PMC5182032_01_ott-10-031Fig2.jpg
f2-ott-10-031
This immunophenotype is compatible with the expression of LGL surface markers, and the aberrant lack of CD5 expression sustained the clonality of the mentioned population (Figure 2).