PMC548298_01
The PCR amplified product for devR gene (513 bps) of M. tuberculosis. (From left to right) Lane M; 100 bp ladder Banglore Genei (India), Lane 1; Negative control, Lane 2; M. tuberculosis H37Rv, Lane 3; Patient's isolate of sensitive M. tuberculosis strain (on first admission), Lane 4; Patient's isolate of MDR M. tuberculosis strain (on readmission), Lane 5; M. kansasii, Lane 6; M. scrofulaceum.
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F1
HIV-negative with normal CXR and there was no AFB in three consecutive (spot-early morning-spot) sputum samples (induced in two occasions). However, the aspirated pus from cutaneous abscess and biopsy of the axillary lymph node showed plenty of M. tuberculosis as culture was confirmed by niacin and nitrate reduction tests, rapid bacteriophage assay (FAST Plaque Tuberculosis kit, BIOTEC Laboratories Ltd, UK) and RFLP analysis (figure 1) using species-specific probes for devR (Differentially expressed virulent gene). Isolates from 2 of 3 consecutive sputum samples and aspirated pus samples from all 5 completely drained abscesses were reconfirmed as the identical M. tuberculosis strain by phage assay and RFLP analysis (figure 1).