PMC5292196_01
Patient with eight lesions on the face, right eyelid, and some lesions on the hands.
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fig1
There were eight lesions on the face that one of them was on the upper eyelid right eye (Figure 1).
Direct smear was positive for leishman bodies (Giemsa stain).
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fig2
Samples were stained by Giemsa 10% and examined under a light microscope and amastigote forms of Leishmania species were observed and CL was confirmed (Figure 2).
RAPD-PCR technique results by OPA4 primer in isolated strain and standards: M- marker (XIV 100 bp), (1) unknown strain, (2) L. tropica (MHOM/IR/IR/99), and (3) L. major (MHOM/IR/75/ER).
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fig3
PCR was performed. PCR was positive for Leishmania major (Figure 3).