PMC5339647_01
(A) Colon biopsy stained with Periodic acid-Schiff stain showing several hyphal structures (black arrows) surrounded by eosinophilic-rich infiltrate called Splendore-Hoeppli phenomenon (magnification x 400). (B) ITS2 Sanger electropherogram showing superposed sequences, suggesting the presence of different fungi in the sample. (C) A Neighbor-Joining tree generated for ITS2 rDNA region sequences identified as Basidiobolus meristosporus (accession number KM24688 to KM246892) with Basidiobolus spp. sequences references using a CLUSTAL X alignment with the optimal criteria set for Distance in MEGA6. Percentage at nodes indicates bootstrap values for 1,000 replicates. Number of sequences that represented our four sequences is indicated in the pie chart (only sequences representing at least three sequences among Basidiobolus bunch of sequences are indicated).
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F1
The main results of the histopathological examination, upon using Periodic acid-Schiff staining, showed large hyphae surrounded by a thick eosinophilic material (Splendore-Hoeppli phenomenon) (Figure 1A).