PMC5771909_01
Surgical preparation of the thrombus after incision of the aortic wall. Note parts of red thrombus with predominance of erythrocytes (1) and white thrombus formation with predominance of fibrin and platelets (2).
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Tourniquet Kit, Covidien, Dublin, Ireland) placed approx. 2 cm cranial of the thrombus (caudal of the A. mesenterica caudalis) was used to suppress aortic blood flow cranial to the thrombus. Two minutes prior to aortotomy, the dog received 110 IE/kg heparin intravenously (Heparin-Natrium 5000 IE, Ratiopharm, Ulm, Germany). The aorta was incised longitudinal approximately 3 cm (Fig. 1). Major parts of the thrombus (Fig. 1) were removed with the forceps.
Demonstration of the thrombectomy via pulling the thrombus (1) out of the aorta (2) using the Fogarty catheter (3).
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Thrombectomy Catheter' (LeMaitre  Single Lumen Embolectomy Catheter, Burlington, VT, balloon diameter 10.5 mm, balloon volume 0.75 mL, length 80 cm) was used (Fig. 2). For this, the catheter was inserted in both directions through the thrombus to the end of the vessel (about 20 cm).
Fogarty catheter with filled balloon to retract thrombus.
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Iliacae internae (Fig. 3).
The thrombus was composed of a fibrin scaffold with interlaced areas of erythrocytes (asterisks), proteinaceous eosinophilic material (thin arrows), and multifocal varying amounts of neutrophilic granulocytes (thick arrows). No histological sings of thrombus organization, for example, fibroblast proliferation (granulation tissue) or re-endothelization, were obvious. Hemalum-eosin staining, Bar 50 mum.
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A formation of granulation tissue indicative for organization of the thrombus was not found, and no bacteria or neoplastic cells were obvious (Fig. 4).