PMC4697352_01
NGS chimerism analysis 41 days after liver transplant. Thirty-two of ninety SNPs discriminated between the donor and recipient. The average percent of donor DNA was 95.1%. Ninety SNPs are shown for (A) the recipient (peripheral blood, pre-transplant), (B) donor (peripheral blood), and (C) recipient (bone marrow, post-transplant) with mean coverage depth for each SNPs, 674, 1,609 and 1,080, respectively. The following markers were used for the chimerism calculation: rs560681, rs891700, rs12997453, rs6444724, rs2046361, rs159606, rs251934, rs338882, rs13218440, rs727811, rs10092491, rs2056277, rs1463729, rs735155, rs740598, rs964681, rs2076848, rs2269355, rs2111980, rs1058083, rs354439, rs873196, rs2016276, rs1382387, rs9905977, rs2292972, rs1736442, rs719366, rs1005533, rs722098, rs2830795, and rs987640.. *discriminating SNPs used in chimerism calculation.. Abbreviations: NGS, next generation sequencing; SNPs, single nucleotide polymorphisms.
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F1
Out of 90 SNPs, 32 sequence variations were observed and read number of variant sequence per read number of expected sequence was calculated to determine percent chimerism (Fig. 1).