PMC3420684_01
Characteristic features of this case of anaplastic lymphoma kinase-negative (ALK-) anaplastic large cell lymphoma (ALCL). (a) Large pleomorphic neoplastic cells with abundant cytoplasm, eccentric nuclei, and brisk mitotic activity (black arrows); Hallmark cells with horseshoe- or kidney-shaped nuclei are present (white arrows). (b) Neoplastic cells show strong membrane and Golgi expression of CD30. (c) Focal immunoreactivity with antibody for the cytotoxic granule protein, Perforin. (d) ALK expression is lacking in this case. (e) High Ki-67 expression, denoting a worse prognosis. (f) Correlated multiparametric flow cytometric analysis demonstrating an abnormal population of T cells (in red) with expression of surface CD4 and lacking surface CD7. Normal T cells (in blue) show normal expression of CD7 and CD4 and include a CD4- subset, which corresponds to the CD8+ T cells. (Black: B cells).
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PMC3420684_01_CRIM.HEMATOLOGY2012-954201.001.jpg
fig1
 Histopathology from core-needle biopsy of the left inguinal lymphadenopathy showed an abnormal infiltrate of relatively cohesive clusters of large pleomorphic cells with morphologic features summarized in Figure 1.