PMC4841994_01
Chest CT scan at the time of admission
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F1
Computed tomography showed bilateral ground glass opacities and consolidation with air bronchogram (Figure 1).
Gel electrophoresis of the resulting products of both RdRpSeq and NSeq assays.
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F2
The RdRpSeq and NSeq gene sequences were amplified (Figure 2) and then the sequence of RdRpSeq PCR product was determined with the Big-Dye terminator cycle sequence kit and an ABI 377A sequencer (Applied Biosystems Inc.).
Phylogenetic analysis of RdRpSeq gene sequence in studied MERS-CoV isolate. The tree was constructed by the Maximum Composite Likelihood method using MEGA6
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F3
Figure 3 shows the phylogenetic tree of the MERS-CoV isolate.
CXR at the 4th day of admission
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F4
With supportive care and antimicrobial agents he became better and no longer needed oxygen therapy at the third day of admission, and chest X ray revealed significant improvement at the 4th day of admission (Figure 4).