PMC5784466_01
(a-f) Transverse, (g) sagittal and (h) dorsal MRI findings for a B-cell lymphoma involving the region of the left lateral aperture in a 10-year-old male cat. The lesion appears as isointense-to-normal grey matter on (a) T2-weighted imaging (T2WI) and (b) fluid-attenuated inversion recovery imaging, and (c) hypointense on T1-weighted imaging (T1WI). (d,g,h) The lesion is homogeneously enhanced. (e) On diffusion-weighted imaging (DWI), the lesion margin shows remarkable hyperintensity relative to the right cerebellar hemisphere. (f) On the apparent diffusion coefficient (ADC) map, the lesion appears hypointense relative to the right cerebellar hemisphere. The ADC value was calculated for the lesion (circle)
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MRI revealed a well-demarcated extra-axial lesion involving the region of the left lateral aperture (Figure 1d,g,h). The lesion appeared as isointense-to-normal grey matter on T2WI and FLAIR imaging, hypointense on T1WI and homogeneously enhanced on T1WI with contrast (Figure 1a-d). T2WI and FLAIR imaging, hypointense on T1WI and with no enhancement on T1WI with contrast (Figure 1a-d). The imaging findings were consistent with oedema in left flocculus and left medulla oblongata. On DWI, the lesion margin exhibited marked hyperintensity relative to the right cerebellar hemisphere (Figure 1e). On the ADC map, the lesion appeared hypointense relative to the right cerebellar hemisphere (Figure 1f).
Findings of cerebrospinal fluid (CSF) analysis for a B-cell lymphoma involving the left lobe of the cerebellum in a 10-year-old male cat. (a) CSF cytology shows lymphoblasts with a high nuclear:cytoplasmic ratio, basophilic cytoplasm, an irregular nuclear membrane, increased nuclear chromatin and unclear nuclei (Giemsa staining, x 400). B-cell lymphoma was diagnosed on the basis of genetic analysis of the lymphoblasts. (b) Electrophoresis of PCR products for CSF lymphoblasts revealed monoclonal proliferation of IgH
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Clinical Laboratories), and the findings revealed an increase in the nucleated cell count to 46/mul (reference interval 0-5/mul). CSF cytology showed the presence of lymphoblasts (Figure 2a), which exhibited a high nuclear:cytoplasmic ratio, basophilic cytoplasm, an irregular nuclear membrane, increased nuclear chromatin and indistinct nuclei. Genetic analysis of the lymphoblasts was conducted at another research laboratory (Canine-Lab); PCR for the assessment of clonality revealed monoclonal proliferation of IgH (Figure 2b).