PMC9113660_01
Colonial appearance and gram staining of T. inchonensis. (A) Colonial appearance of T. inchonensis on a Columbia blood agar plate after 24h and 48h of incubation; (B) Gram staining (bioMerieux, SA) of T. inchonensis isolated from peritoneal dialysate cultures (x1000).
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After 48h of incubation, odourless, yellow-greyish, dry and membrane-like colonies were observed on blood agar (Figure 1A). Gram staining showed straight, gram-positive rods (Figure 1B).
Mass spectra of the bacterial isolate. The isolate strain was identified by VITEK-MS.
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The isolated strain was identified as Tsukamurella species by MALDI-TOF MS with a confidence level of 99.9% (Figure 2).
Phylogenetic trees presenting the relationship between the isolated strain with Tsukamurella strains. The evolutionary history was inferred using the neighbor-joining method. The isolated strain was marked in red. Evolutionary analyses were conducted in MEGA11.
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T. inchonensis (Figure 3). The nucleotide data were registered in the NCBI database with accession numbers of ON004908 (16S ribosomal RNA).