PMC6249398_01
Gram staining of blood culture fluid (A) and pink-pigmented colonies on a chocolate agar plate (B). A, stained following centrifugation. B, incubated in 5% CO2 at 35  C for 2 days.
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The fluid was centrifuged to collect bacteria, and Gram-negative plump coccoid rods were successfully confirmed (Fig. 1A). Over subsequent days, colonies on the chocolate agar demonstrated a pink color (Fig. 1B).