PMC3952391_01
Radiographic images of the patient's chest on admission. Chest radiograph (a) and chest computed tomography (b)
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F1
There were also some areas of nodular infiltrate demonstrating a " tree-in-bud" appearance in left lung [Figure 1].
The restriction fragment length polymorphism pattern of hsp65 amplicon fom Mycobacterium branderi. Lane (1) 100 bp deoxyribonucleic acid size marker, lane (2) fragment digested with HaeIII, lane (3) fragment digested with BsteII
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F2
TB12 primer) and then the PCR product digested using BstEII and HaeIII restriction endonucleases. The obtained digested pattern (BstEII; 235,211 bp: HaeIII; 130,106,80,41,37 bp) was compared with the standard strains published on database [Figure 2]. To further confirm the identification of M. branderi pure colonies were isolated at 25 C and 45 C within 3 weeks of incubation.