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Clinical evaluation by PET-CT imaging. (A) Compared with the image before CAR T, PET-CT evaluation on day 28 showed nearly CMR with a few loci in the liver left. (B) The lymphoma activities were inhibited after CAR T cell infusion, as seen by PET-CT images for nodules at the lung, liver, and right adnexa. CMR, complete metabolic remission; N, nodule.
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However, the adnexa infiltration still existed, and the size decreased from 5 to 2.6 cm, with an SUV of 2.90, similar to that of the liver blood pool (SUVmax = 2.65) ( Figures 1A, B ). T cells were still detected in the ascites, with a value of 8.13 mul-1 ( Figure 2 ). The expanded CAR T cells consisted of a certain percentage of immunotypic memory cells, with the CD3+CD45RA-CCR7+CAR+cells of 46.68 mul-1 on day 11, accounting for most of the CAR+ cells, and decreased to 2.88 mul-1 on day 21; however, these were still detected at 3 months ( Supplementary Figure S1 ).
Circulating CAR T cells were detected both in the blood and the chylous ascites by flow cytometry, which peaked on day 14 in the blood.
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The level of cytokines detected by flow fluorometry generally showed a fluctuation within the normal range of IL-4, IL-6, IL-10, TNFalpha, and IFNgamma, with a moderate rise of IL-6 on the first day after CAR T cell infusion, which was in accordance with the mild CRS ( Supplementary Figure S2 ). The WBCs and neutrophils were reduced sharply after preconditioning and reached the lowest level within the first week after infusion.
PMC9752063_06
Circulating CAR T cells were detected both in the blood and the chylous ascites by flow cytometry, which peaked on day 14 in the blood.
fimmu-13-1007210-g002.jpg
PMC9752063_06_fimmu-13-1007210-g002.jpg
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The level of cytokines detected by flow fluorometry generally showed a fluctuation within the normal range of IL-4, IL-6, IL-10, TNFalpha, and IFNgamma, with a moderate rise of IL-6 on the first day after CAR T cell infusion, which was in accordance with the mild CRS ( Supplementary Figure S2 ). The WBCs and neutrophils were reduced sharply after preconditioning and reached the lowest level within the first week after infusion.