PMC4025151_01
Imprint from bone marrow biopsy. Giemsa stain. x1,000.
cro-0007-0260-g01.jpg
PMC4025151_01_cro-0007-0260-g01.jpg
F1
An imprint from the bone marrow biopsy was performed and showed moderate megaloblastic changes with a few groups of cohesive nonhematopoietic mononuclear cells with round to indented nuclei, homogeneously condensed chromatin, distinct nucleoli and deeply basophilic cytoplasm (fig. 1).
Bone marrow trephine biopsy. a Intertrabecular space completely filled by angiosarcoma. The lesion shows multiple anastomosing vascular channels (HE. x100). b Vascular spaces lined by endothelial cells with mild to moderate atypia, surrounded by spindled neoplastic cells (HE. x400).
cro-0007-0260-g02.jpg
PMC4025151_01_cro-0007-0260-g02.jpg
F2
The bone marrow trephine biopsy revealed replacement of the normal hematopoietic elements by interstitial infiltration of malignant epithelioid cells with abundant eosinophilic cytoplasm, focal spindle-shaped cells lining cleft-like spaces and anastomosing vascular channels (fig. 2).
Immunohistochemical staining of the bone marrow biopsy. a CD31 positivity in neoplastic cells (x400). b Factor VIII positivity in neoplastic cells (x400).
cro-0007-0260-g03.jpg
PMC4025151_01_cro-0007-0260-g03.jpg
F3
An immunohistochemical analysis revealed that tumor cells expressed the endothelial markers CD31, factor VIII (fig. 3) and CD34 with no expression of epithelial markers, such as cytokeratin.