PMC6233691_01
An ulcerated wound on the sole of right foot, measuring 5x4 cm.
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He complained of recurrent wound infection on his right sole (Figure 1).
Morphology of B. pseudomallei colonies on ash down agar after 48 hours incubation at 37 C.. Notes: The wrinkled surface of the colonies was appearing. The colony size ranges from 4 to 10 mm.
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Ashdown agar, these colonies appeared pale pink, and after prolonged incubation became dark purple, dry, and wrinkled in the center (Figure 2). A very strong sweetish-putrid odor was noted, resembling stagnant water.
Result of PCR using a 16S rRNA primer.. Notes:
Escherichia coli ATCC 35218 was used as negative control for this amplification assay. A product of 397 bp amplicon was present, which confirmed B. pseudomallei identification.
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Visualization of a 397 bp-sized DNA bands in electrophoresis with 1% agarose gel confirmed the presence of 16S rRNA gene of B. pseudomallei (Figure 3).