PMC7723488_01
Hematoxylin and eosin- (H&E-) stained section of nasal mucosa (a, b). Immunohistochemical staining of CD20 (c) and CD138 (d). H&E staining showed diffuse proliferation of atypical lymphoid cells with prominent nucleoli and clear cytoplasm (a, b). Immunohistochemical staining revealed that atypical cells were positive for CD20 (c) and negative for CD138 (d).
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fig1
Immunohistochemical staining revealed that the atypical cells were positive for CD20 and negative for CD138 (Figure 1).
H&E-stained section of bladder tumor (a, b). Immunohistochemical staining of CD20 (c) and CD138 (d). H&E staining showed the proliferation of atypical lymphoid cells with abundant basophilic cytoplasm and pleomorphic nucleoli (a, b). Immunohistochemical staining revealed that these atypical cells were negative for CD20 (c) and positive for CD138 (d).
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fig2
Immunohistochemical staining indicated that these atypical cells were negative for CD20 and positive for CD138 (Figure 2).
Sequences of the CDR3 region in the IGHV of the bladder tumor (a) and nasal tumor (b).The sequence of the CDR3 region in both tumors matched.
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fig3
The sequence of the CDR3 region of both tumors matched (Figure 3), indicating that both PBL and DLBCL emerged from a common clonal B cell.
FISH analysis of the MYC of the bladder tumor (a) and nasal tumor (b). The split signal was detected in the bladder tumor, whereas no split signal was detected in the nasal tumor.
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fig4
The split signal was detected in the bladder tumor, but not in the nasal tumor, indicating MYC rearrangement in the bladder PBL, but not in the nasal DLBCL (Figure 4).