PMC3891423_01
Peripheral blood smear showing many spherocytes (Wright-Giemsa stain, x400).
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F1
The peripheral blood smear revealed spherocytosis with reticulocytopenia and pancytopenia (Figure 1).
Bone marrow aspiration smear showing a giant pronormoblast with basophilic cytoplasm, cytoplasmic blebs (Wright-Giemsa stain, x400).
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F2
Bone marrow aspirate smears showed a few giant pronormoblasts with deep blue cytoplasm, pseudopods, and poorly demarcated intranuclear inclusions (Figure 2).
Detection of human parvovirus B19 DNA by polymerase chain reaction (PCR) in blood and bone marrow aspirates. The first PCR resulted in an amplification of 496 base pair (bp) band, confirmed by the nested PCR, where a 232 bp band was amplified. Lane M, DNA size marker; Lane B, blood sample; Lane BM, bone marrow aspirates; Lane N, negative control; Lane P, positive control
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PMC3891423_01_kjim-20-96-g003.jpg
F3
The PCR of the patient's materials resulted in an amplification of the 496 base pair (bp) band, which was confirmed by the nested PCR, where a 232 bp band was amplified (Figure 3).