PMC9588986_01
For patient 1, the number of CMV DNA copies (A) changes in cytokine levels (B), and chest computed tomography (CT) obtained before and after cell infusion (D) are presented in this figure. For patient 2, the changes in CMV DNA copy number (C) and chest CT (E) after NK cell infusion are also presented. (F) indicates that CMV infection-related retinitis improved after NK cell infusion in both patients. NK cells were expanded directly from peripheral blood mononuclear cells (PBMCs) collected from two haploidentical donors, and cultured for 3 weeks by adding irradiated artificial antigen presenting cells (aAPC-NKElixir , a proprietary NK cell expansion technology from Shanghai YaKe Biotechnology Ltd.) weekly. Fresh NK cells were infused on day 21. The remaining NK cells were cryopreserved until infusion. The cryopreserved NK cells were prepared for infusion by thawing in a 37 C water bath, washing once with infusion buffer (0.5% human serum albumin in Plasmalyte A) and resuspending the final cell dose in 100 mL of infusion buffer.
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F1
The changes in CMV DNA copy number (Figure 1C) and chest CT (Figure 1E) after NK cell infusion are presented in Figure 1. The peripheral blood CMV DNA copy number and chest CT improved after the NK cell infusion, and there was no obvious GVHD.