PMC3771455_01
(a) Atypical lymphoid infiltrates associated with marked fibrosis (20x magnification) and (b) atypical cells with irregular nuclear membrane, open chromatin, and distinct nucleoli (200x magnification).
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fig1
The histological sections demonstrated an effaced nodal architecture with proliferation of large atypical lymphoid infiltrates associated with marked fibrosis (Figure 1(a)). The atypical cells showed irregular nuclear membrane, open chromatin, and distinct nucleoli (Figure 1(b)).
(a) Neoplastic cells demonstrating strong CD3 positivity (200x magnification), (b) neoplastic T cells with aberrant CD20 positivity (100x magnification), and (c) neoplastic T cells with aberrant CD79a positivity (100x magnification).
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fig2
The neoplastic cells stained strongly and diffusely positively for CD3 with partial loss of CD5 and CD7 (Figure 2(a)). A subpopulation of the neoplastic cells was positive for CD20 and CD79a (Figures 2(b) and 2(c)).
Flow cytometry demonstrating aberrant T cells expressing CD3 and CD19, weak CD7, and absent CD4, CD8, and CD10 expression.
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fig3
T-cell population, which expressed CD19 along with CD3 and CD20 (Figure 3).