PMC6206519_01
The neoplastic tumor cells are arranged in nests within a myxoid background (H&E, 200x).
CRIPA2018-3462096.001.jpg
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fig1
Microscopic examination revealed a cellular proliferation of neoplastic cells arranged haphazardly, in cords (Figure 1), occasional nests, and focally palisading (Figure 2) and separated by regularly spaced arborizing fibrovascular septa within an extracellular myxoid matrix (Figure 3) with occasional myxoid pool formation (Figure 4).
Focal areas with palisading are identified within the tumor (H&E, 100x).
CRIPA2018-3462096.002.jpg
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fig2
Microscopic examination revealed a cellular proliferation of neoplastic cells arranged haphazardly, in cords (Figure 1), occasional nests, and focally palisading (Figure 2) and separated by regularly spaced arborizing fibrovascular septa within an extracellular myxoid matrix (Figure 3) with occasional myxoid pool formation (Figure 4).
The arborizing fibrovascular septae classic of CCSK are easily seen (H&E, 200x).
CRIPA2018-3462096.003.jpg
PMC6206519_01_CRIPA2018-3462096.003.jpg
fig3
Microscopic examination revealed a cellular proliferation of neoplastic cells arranged haphazardly, in cords (Figure 1), occasional nests, and focally palisading (Figure 2) and separated by regularly spaced arborizing fibrovascular septa within an extracellular myxoid matrix (Figure 3) with occasional myxoid pool formation (Figure 4).
Myxoid pool formation is noted occasionally throughout the tumor (H&E, 200x).
CRIPA2018-3462096.004.jpg
PMC6206519_01_CRIPA2018-3462096.004.jpg
fig4
Microscopic examination revealed a cellular proliferation of neoplastic cells arranged haphazardly, in cords (Figure 1), occasional nests, and focally palisading (Figure 2) and separated by regularly spaced arborizing fibrovascular septa within an extracellular myxoid matrix (Figure 3) with occasional myxoid pool formation (Figure 4).
The tumor cells are strongly and diffusely positive for vimentin.
CRIPA2018-3462096.005.jpg
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fig5
Immunohistochemical stains were positive for vimentin (Figure 5), cyclin D1 (Figure 6), CD99 (Figure 7), TLE1 (Figure 8), and focally positive for Bcl- 2 (Figure 9) in the tumor cells.
Positive nuclear staining for Cyclin D1 in the tumor cells.
CRIPA2018-3462096.006.jpg
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fig6
Immunohistochemical stains were positive for vimentin (Figure 5), cyclin D1 (Figure 6), CD99 (Figure 7), TLE1 (Figure 8), and focally positive for Bcl- 2 (Figure 9) in the tumor cells.
CD99 is diffusely and strongly staining the tumor cells in a membranous pattern.
CRIPA2018-3462096.007.jpg
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fig7
Immunohistochemical stains were positive for vimentin (Figure 5), cyclin D1 (Figure 6), CD99 (Figure 7), TLE1 (Figure 8), and focally positive for Bcl- 2 (Figure 9) in the tumor cells.
TLE1 has strong and diffuse nuclear positivity in the tumor cells.
CRIPA2018-3462096.008.jpg
PMC6206519_01_CRIPA2018-3462096.008.jpg
fig8
Immunohistochemical stains were positive for vimentin (Figure 5), cyclin D1 (Figure 6), CD99 (Figure 7), TLE1 (Figure 8), and focally positive for Bcl- 2 (Figure 9) in the tumor cells.
BCL-2 is highlighting focal areas within the tumor.
CRIPA2018-3462096.009.jpg
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fig9
Immunohistochemical stains were positive for vimentin (Figure 5), cyclin D1 (Figure 6), CD99 (Figure 7), TLE1 (Figure 8), and focally positive for Bcl- 2 (Figure 9) in the tumor cells.
Absent SS18 (SYT) gene break-apart rearrangement on chromosome 18q11.2 by FISH (fluorescence in situ hybridization).
CRIPA2018-3462096.010.jpg
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fig10
FISH for SYT gene rearrangement (Figure 10) was performed and was negative, ruling out a synovial sarcoma.