PMC3483708_01
Preoperative radiograph of 21 and 22 showing a large periapical radiolucency.
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fig1
Periapical radiograph revealed a large diffused periapical radiolucency in relation to 21 and 22 measuring 1.4 cm in diameter (Figure 1).
Periapical defect after flap reflection.
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fig2
Following administration of local anaesthesia, submarginal incision was given 3 mm apical the marginal gingiva and mucoperiosteal flap was reflected (Figure 2).
Blood collection from anticubital vein.
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fig3
Intravenous blood (by venipuncturing of the antecubital vein) was collected (Figure 3) in a 10 mL sterile tube without anticoagulant and immediately centrifuged at 3,000 rpm for 10 minutes.
PRF clot obtained after centrifugation.
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fig4
PRF was easily separated from red corpuscles base (preserving a small RBC layer) using sterile tweezers (Figure 4) just after removal of PPP (platelet-poor plasma) and then transferred into a sterile dappen dish.
PRF mixed with beta-tricalcium phosphate placed into the defect.
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fig5
PRF was mixed with beta-tricalcium phosphate and augmented into the intrabony defect upto the surrounding bone level (Figure 5).