PMC3562653_01
PMC3562653_02
PMC3562653_03
FISH images of normal and abnormal cells using various probes. (a) A normal cell showing 2 green (G), 2 orange (O) and 2 aqua (A) signals for chromosome 12 and loci 13q14.3 and 13q34 on chromosome 13, respectively, using Vysis CLL probe set for CEP12, 13q14.3 and 13q34. (b) A cell showing 2G1O1A signal pattern indicating monosomy 13 using Vysis CLL probe set for CEP12, 13q14.3, and 13q34. (c) Normal cell showing 2 fusion (F) signals for IGH locus (14q32) using Vysis IGH break-apart rearrangement probe. (d) A cell showing 1G1O1F signal pattern indicating IGH rearrangement at 14q32 using Vysis IGH break-apart rearrangement probe. (e) A normal cell showing 2 green and 2 orange signals for chromosomes 11 and 17, respectively, using Vysis CLL probe set for 11q23 (ATM) and 17p13.1 (p53). (f) A normal cell showing 2 orange and 2 green signals for chromosomes 4 and 14, respectively, using Vysis LSI IGH-FGFR3 dual colour dual fusion translocation probe. (g) A normal cell showing 2 green and 2 orange signals for chromosomes 14 and 16, respectively, using Vysis LSI IGH-MAF dual colour dual fusion translocation probe. (h) A cell showing 1G1O2F signal pattern indicating IGH-MAF fusion for t(14;16) using Vysis LSI IGH-MAF dual colour dual fusion translocation probe.
CRIM.GENETICS2013-279801.001.jpg
PMC3562653_03_CRIM.GENETICS2013-279801.001.jpg
fig1
This showed the translocation t(14;16)(q32;q22) in 27% cells (Figure 1).