PMC4405692_01
Enlarged yellowish brown pale liver in the affected canary. Note the loss of coronary adipose tissue and the dark mucoid contents in the intestines.
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F1
The liver was significantly pale (yellow to light brown) and swollen (Fig. 1).
Liver contact smear. Degenerated cells (Black arrows). Note numerous unstained ghost-like bacilli covering all over the smear in the background, (Giemsa, 1000x).
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F2
Some ghost-like unstained micro-organisms resembling acid-fast bacilli were seen in both cytoplasmic background and intracellularly in the liver (Fig. 2) and also in the spleen.
Scratch smear from proventriculus mucosa. Numerous huge bacilli (arrow) identical to Macrorhabdus ornithogaster can be seen, (Giemsa, 1000x).
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F3
In both wet smear and stained smear acquired from proventricular mucosa, many giant bacilli resembling M. ornithogaster were seen (Fig. 3).
High magnification of thin histologic section of the liver. Too many tiny red acid fast bacilli scattered within the tissue, (Ziehl-Neelsen, 1000x).
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F4
Numerous acid fast bacilli were histologically observed using Ziehl-Neelsen stained thin sections of liver (Fig. 4), spleen, lung and kidney.