PMC10202396_01
Microgametocyte 6.4 mum in diameter observed with pigments (x1000 magnification; Giemsa stain - red arrow).
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PMC10202396_01_acmi-5-461.v3-g001.jpg
F1
Giemsa-stained peripheral blood smears revealed normal sized erythrocytes with microgametocytes containing malarial pigments (Fig. 1).
Identification of Plasmodium species by a species-specific nested PCR assay. Lane 1, 100 bp DNA ladder; Lane 2, positive control of Plasmodium falciparum (206 bp); Lane 4, sample positive for Plasmodium vivax (121 bp); Lane 7, negative control.
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PMC10202396_01_acmi-5-461.v3-g002.jpg
F2
Blood sample was subjected to a multiplex nested PCR assay using published primers (Table 1), and an amplified product of 121 bp corresponding with P. vivax was detected by 1.5 % agarose gel electrophoresis using a molecular ladder of 100 bp (Thermo Scientific Gene Ruler 100 bp ladder) (Fig. 2).