PMC5846225_01
Lane 1 - DNA ladder, lane 2 - lytA (319 bp) and lane 7 - ply gene (348 bp) for optochin-resistant bile soluble blood isolate
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F1
Identification by both automated systems VITEK II and MALDI-TOF was S. pneumoniae, and detection of lytA gene and ply gene, encoding for virulence factors autolysin and pneumolysin, respectively, were found to be positive [Figure 1].
Gene sequencing for lyt A (forward primer)
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F2
Further Gene sequencing was done, and the isolate was found to have the synonymous single nucleotide polymorphism type of functional mutation in lytA gene [Figures 2 and 3].
Gene sequencing for lyt A (reverse primer)
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F3
Further Gene sequencing was done, and the isolate was found to have the synonymous single nucleotide polymorphism type of functional mutation in lytA gene [Figures 2 and 3].