PMC4299380_01
RT-PCR using primers located in
TRIP11
exons 14-16 and
PDGFRB
exons 11-12 resulting in amplification of
TRIP11-PDGFRB
fusion transcripts. M: Molecular weight marker, lane 1; 5' TRIP11 and 3' PDGFRB specific primers (patient), lane 2; Replication of 5' TRIP11 and 3' PDGFRB specific primers (patient), lane 3; 5' TRIP11 and 3' PDGFRB specific primers (control), lane 4; 5' KIAA1509 and 3' PDGFRB specific primers (patient), lane 5; 5' KIAA1509 and 3' PDGFRB specific primers (control).
13039_2014_103_Fig1_HTML.jpg
PMC4299380_01_13039_2014_103_Fig1_HTML.jpg
Fig1
As a result, TRIP11-PDGFRB fusion transcript, not KIAA1509- PDGFRB was detected (Figures 1 and 2).
Sequence chromatogram of the
TRIP11-PDGFRB
fusion junction showing the fusion between exon 16 of
TRIP11
and exon 11 of
PDGFRB
. Black arrows indicate the location of primers. The white arrow indicates the location of breakage and reunion. E: exon.
13039_2014_103_Fig2_HTML.jpg
PMC4299380_01_13039_2014_103_Fig2_HTML.jpg
Fig2
As a result, TRIP11-PDGFRB fusion transcript, not KIAA1509- PDGFRB was detected (Figures 1 and 2).