PMC10284151_01
Functional VITT test, markers of platelet activation and coagulation. (A) A flow cytometry-based assay to detect platelet-activating antibodies was performed according to Handtke et al. Levels of CD62p surface expression in healthy donor's platelets stimulated with plasma from the patient in the presence of low (1 UI/ml) and high (100 UI/ml) concentrations of heparin. Plasmatic levels of CD62p (B) and tissue factor (C). The blue area represents the reference range of healthy donors. VITT, vaccine-induced immune thrombocytopenia and thrombosis;
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F1
A flow cytometry-based assay to detect platelet-activating antibodies was performed according to Handtke et al. (Figure 1A). Elevated plasmatic levels of CD62p, released by activated platelets and endothelial cells, and of tissue factor (TF, coagulation factor III), the primary activator of the extrinsic pathway of the coagulation cascade, corroborate the extensive platelet activation and clot formation (Figures 1B,C).