PMC6503770_01
(a) Cross-sections of numerous Aspergillus and Mucorales hyphae. (b) Septate fungal hyphae of mucormycosis. (c) Fruiting bodies (conidiophores) of Aspergillus. (d) Actinomycotic colonies present with associated trabeculae of resorbing bone (H&E, x40)
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F1
Some fungal hyphae were broad, aseptate and ribbon shaped, showing branching at obtuse angles representing Mucorales species, whereas others were thin and septate, showing branching at acute angles representing Aspergillus species [Figure 1]. Fungal spores of varying sizes were observed, exhibiting budding at some places along with fruiting bodies of Aspergillus [Figure 1]. Numerous actinomycotic colonies were also noted along with other bacterial colonies, including both bacilli and cocci arranged in clusters as well as individually in close association with peripheral bony trabeculae [Figure 1].
(a) Fungal hyphae of mucormycosis on GMS staining. (b) Septate aspergillus fungal hyphae on GMS staining. ( x100 original magnification)
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F2
Grocott's methenamine silver staining showed septate Aspergillus hyphae, aseptate Mucorales hyphae and fungal spores along with actinomycotic colonies [Figures 2-5].
Abundant cross-sections of fungal hyphae for aspergillus (red arrow) and mucormycosis (yellow arrow) exhibiting difference in sizes on GMS staining. ( x40 original magnification)
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F3
Grocott's methenamine silver staining showed septate Aspergillus hyphae, aseptate Mucorales hyphae and fungal spores along with actinomycotic colonies [Figures 2-5].
(a and b) Showing fruiting bodies closely associated with bony trabeculae on GMS staining. ( x40 original magnification)
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F4
Grocott's methenamine silver staining showed septate Aspergillus hyphae, aseptate Mucorales hyphae and fungal spores along with actinomycotic colonies [Figures 2-5].
GMS staining observed within the actinomycotic colonies. ( x40 original magnification)
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F5
Grocott's methenamine silver staining showed septate Aspergillus hyphae, aseptate Mucorales hyphae and fungal spores along with actinomycotic colonies [Figures 2-5].