PMC6081519_01
Preoperative panoramic radiograph of the patient.
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She was indicated for a prosthetic implant rehabilitation procedure in the second quadrant after a maxillary sinus lift procedure for atrophy of the maxillary bone at the bicuspid and molar level (1 mm residual bone crest height) in order to collect enough bone to install two endosseous implants (Figures 1 and 2).
Intraoral radiograph (Rinn  collimator) of the surgery site.
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She was indicated for a prosthetic implant rehabilitation procedure in the second quadrant after a maxillary sinus lift procedure for atrophy of the maxillary bone at the bicuspid and molar level (1 mm residual bone crest height) in order to collect enough bone to install two endosseous implants (Figures 1 and 2).
Connective tissue collected directly from the surgery site.
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Then, it was inserted in the Rigeneracons filter with 1 ml of sterile saline for the disaggregation process (Figures 3 and 4).
Tissue graft disaggregation with the Rigeneracons device, according to the manufacturer's instructions: 1 ml of sterile saline solution, performed for 120 seconds with implant contra-angle at 15 NCm and 70 rpm.
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Then, it was inserted in the Rigeneracons filter with 1 ml of sterile saline for the disaggregation process (Figures 3 and 4).
The syringe with progenitor cell-enriched suspension obtained via the periosteum disaggregation process.
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Italy) in order to be grafted into the new subantral cavity (Figure 5). In the meantime, the receiving site was prepared according to the standard protocol used for lateral sinus floor augmentation (Figure 6).
The window elevation.
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Italy) in order to be grafted into the new subantral cavity (Figure 5). In the meantime, the receiving site was prepared according to the standard protocol used for lateral sinus floor augmentation (Figure 6). A resorbable membrane (Bio-Gide , Geistlich Pharma AG, Wolhusen, Switzerland) was positioned in the newly formed subantral cavity to preserve the sinus membrane, and the space was then filled with the blend of PLGA and micrograft (Figures 6 and 7).
The biocomplex graft placement in the maxillary sinus, under the Schneider membrane.
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A resorbable membrane (Bio-Gide , Geistlich Pharma AG, Wolhusen, Switzerland) was positioned in the newly formed subantral cavity to preserve the sinus membrane, and the space was then filled with the blend of PLGA and micrograft (Figures 6 and 7).
Covering the osteotomy access with collagen and resorbable membrane.
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The bone window was covered with collagen sponges (Gingistat , Pierre Rolland Pharmaceutical, Merignac, Aquitaine, France) soaked in the cell suspension and a resorbable membrane (Bio-Gide, Geistlich Pharma, Wolhusen, Switzerland) (Figure 8).
Radiograph taken postimplantation.
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No adverse events occurred (Figure 9).
Hematoxylin/eosin staining of samples at 4 months (a, b) after grafting with the Rigenera system. (a) 10x magnification, (b) 40x magnification.
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The collected tissues were fixed in a 10% formalin solution and then prepared for microscopic observation in order to determine the ossification grade (Figure 10). In fact, at 40x magnification (Figure 10(b)), lamellar bone formation was observed, as seen by the presence of a typical Haversian system with the deposition of a calcified matrix.
Intraoral radiograph taken after 3 years.
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