PMC3109785_01
Periodic acid Schiff (PAS) stain, 400x magnification, shows an enlarged glomerulus with deposits which are PAS negative and occasional PAS positive hyaline caps. Tubules and interstitium are normal
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F0001
These deposits were negative for periodic acid Schiff (PAS) [Figure 1], silver methanamine [Figure 2], and Congo red stains [Figure 3]. There was associated PAS positive peripheral hyalinosis in some glomeruli [Figure 1].
Silver stain, 400x view shows deposits are silver negative
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F0002
These deposits were negative for periodic acid Schiff (PAS) [Figure 1], silver methanamine [Figure 2], and Congo red stains [Figure 3].
Deposits were not congophilic
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F0003
These deposits were negative for periodic acid Schiff (PAS) [Figure 1], silver methanamine [Figure 2], and Congo red stains [Figure 3].
Immuno-fluorescence stain: the deposits were negative for all IgG, IgM and complements
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F0004
Direct immunofluorescence study showed focal and segmental deposits of C3c corresponding to peripheral hyalinosis [Figure 4].
Electron microscopic study of renal biopsy specimen fixed using gluteraldehyde, viewed under 40,000x magnification revealed abundant subendothelial deposits of large fibers (97nm in width) with long spacing striations suggestive of collagen fibers; confirming the diagnosis of collagenous glomerulopathy
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F0005
The biopsy cores fixed with gluteraldehyde revealed abundant subendothelial deposits of large fibers (97 nm in width) with long spacing striations (25-30 nm), which were characteristic of collagen fibers [Figure 5].
PMC3109785_02
Kidney biopsy slide stained with hemotoxylin-eosin stain and periodic acid Schiff stain (PAS), viewed under 400x magnification shows an enlarged glomerulus with increased mesangial matrix and mesangial interposition with diffuse membrane thickening. Tubules and interstitium are normal
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F0006
Light microscopy and immunoflourescence revealed features suggestive of collagenofibrotic glomerulopathy [Figure 6], and was confirmed by electron microscopy [Figure 7].
Electron microscopic study of renal biopsy specimen fixed using gluteraldehyde, viewed under 40,000x magnification revealed abundant subendothelial deposits of large fibers (97nm in width) with long spacing striations suggestive of collagen fibers; confirming the diagnosis of collagenous glomerulopathy
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F0007
Light microscopy and immunoflourescence revealed features suggestive of collagenofibrotic glomerulopathy [Figure 6], and was confirmed by electron microscopy [Figure 7].