PMC10351519_01
Weissella confusa Gram stain morphology from a sheep blood agar plate (x1000).
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Gram-positive coccobacilli were isolated from 3 bottles (1 pair of FA-Plus and FN-Plus bottles and another FN-Plus bottle) within 48 hours of incubation at 35 C with 7% CO2 (Figure 1).
Colonies of Weissella confusa on a sheep blood agar plate grown at 35 C with 7% CO2 after 24hr (left) and 48hr (right) of incubation.
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Gray-white and small-sized alpha-hemolytic colonies were observed on sheep blood agar (Figure 2).
Biochemical reaction spectrum of Weissella confusa.
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The biochemical reaction profile is shown in the following figure (Figure 3).
Mass spectrogram of Weissella confusa.
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Weissella confusa through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS, VITEK MS system, bioMerieux Inc.), with a coincidence rate of 99.9% (Figure 4). We conducted in vitro drug susceptibility experiments using both the disk diffusion method (using sheep blood Mueller-Hinton agar plates) and the microbroth dilution method.