PMC4207494_01
Indirect immunofluorescence on HEp-2 cells performed with an autoimmune hepatitis serum and demonstrating centromere staining.
CRII2013-742080.001.jpg
PMC4207494_01_CRII2013-742080.001.jpg
fig1
Antinuclear antibody (ANA) by IFA (1 : 320 titer) on Hep-2 cells (HEp-2000 IgG fluorescent ANA-Ro test system, Immunoconcepts, USA) revealed anticentromere antibodies (Figure 1) showing 40-60 discrete speckles distributed over the nucleus, either dispersed or gathered closely together on the chromosomes of cells undergoing division.