PMC9826603_01
Fluorescence histograms of the patient's red blood cells (RBCs) which were incubated with FITC-conjugated monoclonal anti-D. Blood samples were collected once per day over four consecutive days and analyzed the day after they were collected. The first dose of anti-D Ig was administered approximately 66 hours after the transfusion of RhD positive PRBC unit. The following 9 doses were given intravenously at 68, 71, 76, 84, 88, 92, 98, 105 and 110 hours over a period of 44 hours. (A) The first blood sample before administration of the first dose of therapeutic high dose anti-D immunoglobulin showed 6.7% RhD positive RBCs inadvertently transfused. (B) The second sample showed 4.6% RhD positive RBCs. (C) The third sample showed 1.0% RhD positive RBCs. (D) The fourth sample showed 0% RhD positive RBCs.
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Flow cytometry was utilized to monitor the amount of RhD positive RBCs in the woman's blood circulation (Figure 1A-D).