PMC7647743_01
Flow cytometry of the lymph node showing atypical T cells positive for CD2, CD7 (dim), and CD8, with negative surface CD3, CD4, and CD5.
CRIHEM2020-8890502.001.jpg
PMC7647743_01_CRIHEM2020-8890502.001.jpg
fig1
T cells which were positive for CD2, CD7 (dim), and CD8, while negative for surface CD3, CD5, and CD4 (reference commercial laboratory 1, Figure 1).
Biopsy of the left cervical lymph node. Hematoxylin and eosin (a, b), immunohistochemistry staining (c-j), and in situ hybridization (l). (a, b) H&E sections showing sheets of pleomorphic atypical medium-to-large lymphocytes. (c) CD2. (d) CD3. (e) CD4. (f) CD5. (g) CD7. (h) CD8. (i) CD20. (j) CD25. (k) Ki67. (l) Epstein-Barr virus- (EBV-) encoded RNAs (EBER) (original magnification, x200 (a, c-l) and x400 (b)).
CRIHEM2020-8890502.002.jpg
PMC7647743_01_CRIHEM2020-8890502.002.jpg
fig2
The hematoxylin and eosin staining of the lymph node revealed complete effacement of the lymph node architecture and replaced by sheets of pleomorphic atypical medium-to-large lymphocytes (Figure 2). The atypical lymphocytes exhibited irregular nuclear contour, condensed chromatin, and distinct nucleoli. By immunohistochemical staining (Figure 2), the atypical lymphocytes were positive for CD3, CD8, and CD25; variably positive for CD2, CD7, and TIA-1; and negative for CD4, CD5, CD20, CD34, TdT, EBER, and CD30.