PMC7606916_01
Graphical representation of mutational acquisition/expansion. (A) Patient 1, (B) patient 2, and (C) patient 3. Clonal size was estimated based on variant allelic frequency (VAF) for mutations detected by NGS, allelic ratio for FLT3, percentage of BCR-ABL to ABL transcripts, and number of aberrant metaphases in karyotype. Clones could have coexisted or be mutually exclusive, mutations with high clonal size at the same given point of time were considered to be coexisted, for other mutations, the two possibilities were represented. At last relapse/progression in (A,B), the two branch graphs represent the possibility of coexistence or mutually exclusivity of FLT3 mutation and BCR/ABL.
fonc-10-588876-g001.jpg
PMC7606916_03_fonc-10-588876-g001.jpg
F1
Upon referral to our institution, repeat bone marrow biopsy showed 38% blasts, complex cytogenetics with t(9,22), BCR-ABL1 fusion transcript (63.9%), FLT3-ITD (allelic ratio 0.48), RUNX1, WT1 (three distinct mutations) (Figure 1C).
PMC7606916_02
Graphical representation of mutational acquisition/expansion. (A) Patient 1, (B) patient 2, and (C) patient 3. Clonal size was estimated based on variant allelic frequency (VAF) for mutations detected by NGS, allelic ratio for FLT3, percentage of BCR-ABL to ABL transcripts, and number of aberrant metaphases in karyotype. Clones could have coexisted or be mutually exclusive, mutations with high clonal size at the same given point of time were considered to be coexisted, for other mutations, the two possibilities were represented. At last relapse/progression in (A,B), the two branch graphs represent the possibility of coexistence or mutually exclusivity of FLT3 mutation and BCR/ABL.
fonc-10-588876-g001.jpg
PMC7606916_03_fonc-10-588876-g001.jpg
F1
Upon referral to our institution, repeat bone marrow biopsy showed 38% blasts, complex cytogenetics with t(9,22), BCR-ABL1 fusion transcript (63.9%), FLT3-ITD (allelic ratio 0.48), RUNX1, WT1 (three distinct mutations) (Figure 1C).
PMC7606916_03
Graphical representation of mutational acquisition/expansion. (A) Patient 1, (B) patient 2, and (C) patient 3. Clonal size was estimated based on variant allelic frequency (VAF) for mutations detected by NGS, allelic ratio for FLT3, percentage of BCR-ABL to ABL transcripts, and number of aberrant metaphases in karyotype. Clones could have coexisted or be mutually exclusive, mutations with high clonal size at the same given point of time were considered to be coexisted, for other mutations, the two possibilities were represented. At last relapse/progression in (A,B), the two branch graphs represent the possibility of coexistence or mutually exclusivity of FLT3 mutation and BCR/ABL.
fonc-10-588876-g001.jpg
PMC7606916_03_fonc-10-588876-g001.jpg
F1
Upon referral to our institution, repeat bone marrow biopsy showed 38% blasts, complex cytogenetics with t(9,22), BCR-ABL1 fusion transcript (63.9%), FLT3-ITD (allelic ratio 0.48), RUNX1, WT1 (three distinct mutations) (Figure 1C).