PMC5681518_01
Multicolor fluorescence in situ hybridization assays were performed on the pathogenic synovial fluid after this latter was formalin fixed and paraffin embedded. Large numbers of viable coccobacilli representing the causative Kingella kingae strains are visualized in red using a rhodamine-labeled probe targeting a consensus sequence of the bacterial 16S rRNA gene (EUB388 III, top left), in green using an FITC-labeled probe targeting the K. kingae-specific V1 region of the 16S rRNA gene (top right), and in blue using a DAPI probe to label deoxyribonucleic acid (middle left). An internal negative control was performed by using a bacterial non EUB388 probe (middle right). The merged image was obtained summing the four abovementioned images (bottom left).
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PMC5681518_01_fped-05-00230-g001.jpg
F1
The organism was also identified by multicolor fluorescence in situ hybridization specific for K. kingae (Figures S1 and S2 in Supplementary Material), which revealed the presence of large numbers of viable coccobacilli located within the synovial fluid (Figure 1).