PMC6433936_01
(a) MD child patient, the skin color of the lower limbs was slightly purple, edema in both lower limbs could be detected; (b) matched child, no obvious abnormality; (c,d) father patient, edema in right lower limb could be detected; the left lower limb became normal post plastic surgery.
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F2
The skin color of the lower limbs was slightly purple (Figure 2a). The father was also advised to order stretch socks to relieve symptoms (Figures 2c,d) (Supplementary Video 1). The structure and skin color of the lower limbs was normal, and no other physical dysfunction was detected (Figure 2b).
HE stain: (a) foot dorsum of MD child (40 times magnification); (b) lateral low-limb skin of MD child (40 times magnification); (c) foot dorsum of matched child (40 times magnification); (d) lateral low-limb skin of matched child (40 times magnification). (e,f) lymphatic vessels found within the field of vision (e) foot dorsum of MD child (100 times magnification); (f) lateral low-limb skin of MD child (100 times magnification).
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F4
PMC6433936_02
Sanger verification results of the family (II-2, FLT4: c.3075G>A). MDC, MD child; MC, matched child.
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F5
The result of the matched child showed normal in the FLT4 (Figure 5).