PMC5424186_01
Liver biopsy specimen. Portal triad in center. Hepatocytes have pale faintly granular eosinophilic to clear cytoplasm. Note that some nuclei have cleared chromatin (nuclear glycogenosis), which can be seen in various metabolic conditions, such as diabetes mellitus (Hematoxylin and Eosin staining at 400x magnification).
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fig1
Nuclear glycogenosis was observed (Figure 1).
(a) 200x magnification PAS stain (without diastase) to show bright red staining of hepatocyte cytoplasm. (b) 200x PAS stain with diastase (to break down glycogen) to show marked decrease in bright red staining of hepatocyte cytoplasm, indicating the amount of glycogen present before. The clear vacuoles are consistent with lipid (steatosis).
CRIHEP2017-7651387.002.jpg
PMC5424186_01_CRIHEP2017-7651387.002.jpg
fig2
Periodic acid-Schiff stain revealed glycogen accumulation which dissolved upon diastase application (Figure 2).