PMC9448255_01
Positron emission tomography (PET-CT): A and B) Increased glycolytic metabolism in several lymph nodes, some of them forming conglomerates, above and below the diaphragm. Increased glycolytic metabolism in the spleen, liver and bones, suggestive of lymphoproliferative disorder.
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The positron emission tomography revealed an increased glycolytic metabolism in several lymph nodes, some of them forming conglomerates, both above and below the diaphragm, as well as increased glycolytic metabolism in the spleen, liver and bones, suggestive of lymphoproliferative disorder (Figures 1A and 1B).
Photomicrographs of lymph node section: A) Histological panoramic view of the left inguinal lymph node stained with hematoxylin eosin showing architectural modifications and cellular proliferation with poorly defined nodules; B) Immunohistochemistry stain: CD15+ (B), CD30+ e PAX-5+, CD20 (-), CD3 (-). Nodular sclerosing Hodgkin's lymphoma x 100; C) Positive immunohistochemistry reaction for Leishmania spp. antigens mononuclear cells x 400.
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The biopsy of the inguinal lymph node revealed a high-grade nodular sclerosing Hodgkin's lymphoma (Figures 2A and 2B) and positive immunohistochemistry for Leishmania spp. (Figure 2C).
Plotting of Hsp70-HRM assays. Melting profiles of Leishmania reference strains and samples from the patient. Panels show the melting profiles with organized data in normalized melting curves (A) and normalized difference curves (B). (INF): L. (L.) infantum chagasi; (AMA): L. (L.) amazonensis; (LAI): L. (Viannia) lainsoni; (BRA): L. (V.) braziliensis; (GUY): L. (V.) guyanensis; (NAI): L. (V.) naiffi; (SHA): L. (V.) shawi; (SBF): patient.
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According to the dissociation profile by HRM, the Leishmania strain was characterized as being L. (L.) amazonensis (Figures 3A and 3B ). 