PMC6530217_01
Adamantinoma-like EWS: ill-defined lesion composed of cells arranged in lobules and nests (H and E x 100).
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fig1
Immunoreactivity for p16, WT1, chromogranin, S100, EMA, vimentin, and desmin were negative in the lesional cells (Figures 1, 2, 3, and 4).
Adamantinoma-like EWS: H&E x400.
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fig2
Immunoreactivity for p16, WT1, chromogranin, S100, EMA, vimentin, and desmin were negative in the lesional cells (Figures 1, 2, 3, and 4).
Adamantinoma-like EWS: prominent squamoid morphology with peripheral palisading. ((a) H&E X 200; (b) H&E X 400).
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fig3
Immunoreactivity for p16, WT1, chromogranin, S100, EMA, vimentin, and desmin were negative in the lesional cells (Figures 1, 2, 3, and 4).
FISH image showing separation of orange and green fluorescence indicating presence of EWSR1 translocation.
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fig5
Immunoreactivity for p16, WT1, chromogranin, S100, EMA, vimentin, and desmin were negative in the lesional cells (Figures 1, 2, 3, and 4).
RT-PCR showing ESW-FLI1 fusion product.
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fig6
EWSR testing with break-apart analysis confirmed EWSR1 and FLI-1 rearrangements (Figure 5).