PMC5850707_01
Antibody identification showing pan-reactive results using ID-Diapanel
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F1
AHG phase with negative autocontrol. Antibody identification using ID-Diapanel (Diamed Cressiur sur Morat, Switzerland) showed 4+ pan-reactive reaction with all 11 panel cells with negative autocontrol giving suspicion of antibody against High Frequency antigen (HFA) [Figure 1].
Antibody screen using (a) saline washed ID-Diacell panel cells and (b) unwashed panel cells
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F2
To confirm the nature of drug (anti-preservative)-induced antibodies, reagent red cells were washed thrice with saline and ICT was repeated which came negative with washed reagent red cells [Figure 2].