PMC9432383_01
A medium echoic mass revealed by echocardiography.
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Echocardiography revealed a medium echoic mass (2.7x1.4 cm in diameter and approximately 3.2 cm2 in area) at the posterior mitral valve tip, which swung with the cardiac cycle (Figure 1).
Morphological, pathological and microbiological data of valve tissue and vegetation after mitral valve and vegetation resection.
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Large polypoid vegetation was found attached to the posterior leaflet of the mitral valve in the resected specimen (Figure 2A). Large polypoid excrescences of fibrin and platelets along the disrupted endocardial surface were seen in the pathological sections of valve tissue and vegetation (Figure 2B). Gram staining of the pathological sections revealed that the vegetations were acutely inflamed, with areas of valvular and leukocyte necrosis containing colonies of bacteria (Figure 2C). mNGS was retrospectively performed to detect pathogens in the mitral valve and vegetation wax tissue, using the following steps: DNA was extracted using the Generic Rapid Genomic DNA Extraction Kit (Lifefeng: DK806-02), quantified by