PMC10287526_01
Timeline of the case report under study.= (CC : ClinCheck ; T0: baseline:before the placement of the clear aligner; T1: 24 hours after the clear aligner placement; T2: 72 hours after the clear aligner placement; T3: 7 days after the clear aligner placement).
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fig1
Intraoral examination revealed a diminished overjet and overbite, rotation of the lower right second premolar (45) and upper left first premolar (24), absence of the upper left second premolar (25), an edentulous space between the lower left first premolar and the second molar (absence of the teeth 35 and 36, where it was decided to open space only for one teeth), positive buccolingual misposition of the upper left central, a tendency to a Class III malocclusion, and a deviation of the lower midline 2 mm to the left (see the X-ray imaging in the Supplementary Figure S1). The orthodontic intervention timeline is depicted in Figure 1.
Different views of the case report before the orthodontic treatment: (a) Pretreatment photographic recording (September 20th, 2018) and (b) Posttreatment photographic recording (August 4th, 2021).
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fig2
Initially, only clear aligners were being used, without any kind of auxiliaries (Supplementary Figure 2).
Levels of (a) interleukin-1beta in gingival crevicular fluid samples and (b) pain intensity using visual analogue scale at baseline and after 1, 3, and 7 days of orthodontic treatment. a denotes significant differences compared to 1 day; b denotes significant differences compared to 3 days; and c denotes significant differences compared to 7 days (p < 0.05).
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fig3
Figure 3). For this reason, a sectional fixed appliance with an open coil-spring connecting teeth 24 and 26 was used (Supplementary Figure 4).
Graphical display of (a) interleukin-1beta levels in gingival crevicular fluid samples; and (b) pain scores reported by the patient, at the baseline and after 1, 3, and 7 days, grouped according to the type of stimulation (i.e., no stimulation, vibration, and photobiomodulation).
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fig4
Figure 3). For this reason, a sectional fixed appliance with an open coil-spring connecting teeth 24 and 26 was used (Supplementary Figure 4).
Graphical display of (a) interleukin-1beta levels in gingival crevicular fluid samples; and (b) pain scores reported by the patient, at the baseline and after 1, 3, and 7 days, grouped according to the complexity of the movement promoted by the orthodontic appliances (i.e., complex and moderate movements) (p < 0.05).
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fig5
After IL-1beta collection and pain measurement at the baseline, the coil-spring between the teeth 24 and 26 was (re) activated by the length of a bracket (see Supplementary Figure S5 for further information on spring-coil specificities).