PMC6376207_01
PMC6376207_02
Schematic overview of cultivation protocol and automated phenotyping protocol. (A) Plants were cultivated for 20 days prior to transplantation in control conditions (FS-WI, PSI, Czechia) and were further kept in the same conditions for the next 19 days (DAT, days after transplantation). Eight types of protein hydrolysates (A-G, I) plus control treatment were applied twice to tomato plants by spraying 5 and 12 days after transplantation. Plant phenotypic measurements were performed during the experiment using PlantScreenTM Modular System installed in semi-controlled greenhouse environment conditions in PSI Research Center (PSI, Drasov, Czechia). (B) Plant handling and automated phenotyping protocol. Tomato plants were transferred to PlantScreenTM Modular System and automated phenotyping protocol was initiated. Prior to and following the protein hydrolysates application, plants were regularly screened using the calibrated top and side view RGB camera and kinetic chlorophyll fluorescence camera for photosynthetic performance quantification. Plants were regularly watered and weighed using the automated watering and weighing station.
fpls-10-00047-g001.jpg
PMC6376207_03_fpls-10-00047-g001.jpg
F1
Photosynthetic performance in the plants was assessed by quantifying the rate of photosynthesis at different photon irradiances using the light curve protocol which was proven to provide detailed information on ChlF under stress, information on photosynthetic performance in many studies dealing with plants' stress and to quantify the rate of photosynthesis at different light irradiances (Supplementary Figure S1).
PMC6376207_03
Schematic overview of cultivation protocol and automated phenotyping protocol. (A) Plants were cultivated for 20 days prior to transplantation in control conditions (FS-WI, PSI, Czechia) and were further kept in the same conditions for the next 19 days (DAT, days after transplantation). Eight types of protein hydrolysates (A-G, I) plus control treatment were applied twice to tomato plants by spraying 5 and 12 days after transplantation. Plant phenotypic measurements were performed during the experiment using PlantScreenTM Modular System installed in semi-controlled greenhouse environment conditions in PSI Research Center (PSI, Drasov, Czechia). (B) Plant handling and automated phenotyping protocol. Tomato plants were transferred to PlantScreenTM Modular System and automated phenotyping protocol was initiated. Prior to and following the protein hydrolysates application, plants were regularly screened using the calibrated top and side view RGB camera and kinetic chlorophyll fluorescence camera for photosynthetic performance quantification. Plants were regularly watered and weighed using the automated watering and weighing station.
fpls-10-00047-g001.jpg
PMC6376207_03_fpls-10-00047-g001.jpg
F1
Photosynthetic performance in the plants was assessed by quantifying the rate of photosynthesis at different photon irradiances using the light curve protocol which was proven to provide detailed information on ChlF under stress, information on photosynthetic performance in many studies dealing with plants' stress and to quantify the rate of photosynthesis at different light irradiances (Supplementary Figure S1).
Color segmented side view Red Green Blue (RGB) images of tomato plants prior to and upon PHs application. (A) Side view (120 ) RGB image of the tomato plants over the time of phenotyping period (D1-D15). (B) Projected shoot area over time of phenotyping period. Values represent the average of six biological replicates per treatment. Error bars represent standard deviation. T1 and T2 correspond to days of protein hydrolysates application by foliar spraying. (C) Digital biomass quantified over time of phenotyping period. Values represent the average of six biological replicates per treatment. Error bars represent standard deviation. T1 and T2 correspond to days of protein hydrolysate application by foliar spraying. (D) Comparison of relative growth rate for the different treatments quantified over phenotyping period following the protein hydrolysate treatments (DAT 6-DAT 15). Values represent the average of six biological replicates per treatment. Error bars represent standard deviation.
fpls-10-00047-g002.jpg
PMC6376207_03_fpls-10-00047-g002.jpg
F2
To assess digital biomass of the plants, RGB imaging was done from top view (RGB2) and side view from multiple angles (RGB1) (Supplementary Figure S2).