PMC7497832_01
(a) Alignment of ORF3a protein of 2019-nCoV Hu-1 and SARS coronavirus. Transmembrane and Golgi localization motif are underlined in orange and purple, respectively. O-glycosylation sites are in green. E191G and G251V mutations (red) are shown in Taiwan and US-CA2, Sweden01, Italy-isl isolates. (b) Schematic representation of 2019-nCoV ORF3a protein. Both E191G and G251V mutations are in cytoplasmic region of Orf3a. (c) Mutation in tryptophan 913 (W913) is located in the thumb domain of RdRp. Structure of viral RNA-dependent RNA polymerase (RdRp) is based on SARS.
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PMC7497832_01_acmi-2-133-g001.jpg
F1