PMC10224627_01
(a) After admission, there showed a cloudy and yellowish CSF. (b and c) Head CT scan showing the decreased density of brain parenchyma, and the narrowed cistern and sulcus, which indicate diffuse swelling of the brain. (d) The physical fragment of N. fowleri was 134 bp. The results were consistent with the electrophoretic bands of the patient (Lane 1), and the negative control (Lane 2) had no bands. It is indicated that Naegleria fowleri exists in this sample. (e) mNGS results show that a total of 23,834 specific reads of N. fowleri were detected in CSF, the coverage was 6.08%.
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Sixteen hours after admission, CT revealed diffuse edema of the whole brain (Figure 1b and c). A total of 23,834 specific sequence reads of N. fowleri were detected by mNGS (Figure 1e). The pathogen was then confirmed by PCR (Figure 1d) using the specific primers F (TCTAGAGATCCAACCAATGG) and R (GTCTTTGTGAAAACATCACC).