PMC6076969_01
CT scan of the abdomen showing the right adrenal tumor ((a), arrow) and the left adrenal tumor ((b), arrow) prior to adrenalectomy.
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fig1
While no abnormalities were seen within the urogenital tract, bilateral, irregular, heterogeneous large adrenal masses (Figure 1) measuring 4.7 cm (R) and 1.6 cm (L) were noted.
Western blot of HIF2alpha, HA-VHL, and GAPDH expression in normal oxygen (left) and hypoxic (right) conditions. VHL-null 786-O cells were transfected and clonally selected for stable expression of either WT or Y175C VHL or GFP (null), as indicated.
CRIE2018-8967159.002.jpg
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fig2
We detected HIF2alpha expression in the control VHL-null cell line, while stable overexpression of either WT or Y175C VHL resulted in the disappearance of HIF2alpha (Figure 2, left panel). As expected, the WT VHL lost the ability to induce HIF2alpha degradation in hypoxia (Figure 2, right panel).