PMC4338389_01
Bone marrow smears at the time of diagnosis (stained by May-Grunwald and Giemsa). Atypical mast cells presented alterations in size and in shape; they presented often with few or numerous metachromatic granules and often contained bilobed nucleus.
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fig1
She had 50-60% infiltration of atypical MCs on bone marrow smears (Figure 1) and 70-80% on biopsy.
Flow cytometric analysis of expression of CD45, CD2, and CD25 on CD117+ neoplastic mast cells on bone marrow blood at diagnosis.
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fig2
CD117, positivity of CD2, and negativity of CD25 antigen (Figure 2); the morphological examination of peripheral blood smear showed <1% of MCs. Clinical and instrumental examinations excluded organomegaly and any signs of organ impairment; the skin was free of maculopapular lesions, so she was diagnosed as aleukemic variant of MCL.