PMC3420440_01
Direct wet mount preparation of tissue showing nodal sporangiophore with umbrella-shaped appearance of sporangium (40x).
CRIM.OTOLARYNGOLOGY2011-382473.001.jpg
PMC3420440_01_CRIM.OTOLARYNGOLOGY2011-382473.001.jpg
fig1
To our surprise, umbrella-shaped empty sporangia along with underdeveloped rhizoids and nodal sporangiophores were also visible in wet mount preparations (Figure 1).
Thin, septate hyphae with vesicle and conidia along with aseptate hyphae and sporangium in KOH preparation from tissue (40x).
CRIM.OTOLARYNGOLOGY2011-382473.002.jpg
PMC3420440_01_CRIM.OTOLARYNGOLOGY2011-382473.002.jpg
fig2
Thin, septate hyphae with vesicle and conidia along with aseptate hyphae and sporangium in KOH preparation were seen in the same field (Figure 2).
SDA plate on the 5th day showing white, cottony, fluffy growth of Zygomycetes species superimposed on powdery, granular growth of Aspergillus species.
CRIM.OTOLARYNGOLOGY2011-382473.003.jpg
PMC3420440_01_CRIM.OTOLARYNGOLOGY2011-382473.003.jpg
fig3
One was mat-like initially having rugose texture which changed to granular and powdery with yellowish-green surface pigment; another was cottony, woolly, and fluffy (Figure 3).
LCB preparation from mixed culture showing ruptured sporangium with developing vesicle in the same field (40x).
CRIM.OTOLARYNGOLOGY2011-382473.004.jpg
PMC3420440_01_CRIM.OTOLARYNGOLOGY2011-382473.004.jpg
fig4
LCB preparation from mixed culture showing ruptured sporangium with developing vesicle in the same field in Figure 4.
PAS staining of tissue section showing thin, dichotomous hyphae intermingled with wide, irregular aseptate hyphae (100x).
CRIM.OTOLARYNGOLOGY2011-382473.005.jpg
PMC3420440_01_CRIM.OTOLARYNGOLOGY2011-382473.005.jpg
fig5
The histopathological report endorsed our findings showing broad and aseptate hyphae suggestive of Zygomycetes sp. and thin, septate hyphae with dichotomous branching suggestive of Aspergillus sp. (Figure 5).