PMC10126733_01
a Kidney biopsy from case 1 showed by light microscopy focal necrotizing and crescentic features. Immunofluorescence (IF) microscopy revealed global coarse granular mesangial deposits of 2+ intensity for IgG (b), C3, kappa, and lambda. Immunoperoxidase staining for DNAJB9 was positive in the glomeruli (c), and electron microscopy (EM) showed fibrillary deposits in the mesangium (d). e The kidney biopsy from case 2 showed by light microscopy mild mesangial proliferation and focal crescentic features. IF microscopy revealed global coarse granular mesangial and glomerular capillary loop deposits of 3 + intensity for IgG (f), C3, kappa, and lambda, and 1 + for C1q, as well as deposits along tubular basement membranes (TBMs) and arteriolar vessel walls. Immunoperoxidase staining for DNAJB9 was positive in the glomeruli (g) and focally along the TBMs and arteriolar vessel walls. h EM showed globally distributed fibrillary deposits in the mesangium and along the glomerular capillary walls. In total, the kidney biopsies from both patients revealed the presence of FGN. Paraffin sections were stained with periodic acid-Schiff (a, e) and DNAJB9 (c, g). Original magnifications for (a, b, c, e, g) at x400; for (f) at x600; for (d) at x8,000; and for (h) at x3,000.
gdz-0003-0069-g01.jpg
PMC10126733_02_gdz-0003-0069-g01.jpg
F1
By light microscopy, glomeruli showed mild mesangial proliferation, of which eight contained cellular crescents and two contained fibrocellular crescents (shown in Fig. 1e-h).
PMC10126733_02
a Kidney biopsy from case 1 showed by light microscopy focal necrotizing and crescentic features. Immunofluorescence (IF) microscopy revealed global coarse granular mesangial deposits of 2+ intensity for IgG (b), C3, kappa, and lambda. Immunoperoxidase staining for DNAJB9 was positive in the glomeruli (c), and electron microscopy (EM) showed fibrillary deposits in the mesangium (d). e The kidney biopsy from case 2 showed by light microscopy mild mesangial proliferation and focal crescentic features. IF microscopy revealed global coarse granular mesangial and glomerular capillary loop deposits of 3 + intensity for IgG (f), C3, kappa, and lambda, and 1 + for C1q, as well as deposits along tubular basement membranes (TBMs) and arteriolar vessel walls. Immunoperoxidase staining for DNAJB9 was positive in the glomeruli (g) and focally along the TBMs and arteriolar vessel walls. h EM showed globally distributed fibrillary deposits in the mesangium and along the glomerular capillary walls. In total, the kidney biopsies from both patients revealed the presence of FGN. Paraffin sections were stained with periodic acid-Schiff (a, e) and DNAJB9 (c, g). Original magnifications for (a, b, c, e, g) at x400; for (f) at x600; for (d) at x8,000; and for (h) at x3,000.
gdz-0003-0069-g01.jpg
PMC10126733_02_gdz-0003-0069-g01.jpg
F1
By light microscopy, glomeruli showed mild mesangial proliferation, of which eight contained cellular crescents and two contained fibrocellular crescents (shown in Fig. 1e-h).