PMC3526373_01
MRI and PET over MRI superimposed brain images of the patient who had been infused autologous cytolytic T cells expressing IL13 zetakine and HSV1-tk genes. Images were acquired approximately two hours after [18F]FHBG injection. The patient had a surgically ressected tumor (1) in the left corner and a new non-ressected tumor in the center (2), near corpus callosum of his brain. The infused cells had localized at the site of tumor 1 and also trafficked to tumor 2. [18F]FHBG activity is higher than the brain background at both sites. Background [18F]FHBG activity is low within the Central Nervous System due to its inability to cross the blood brain barrier. Background activity is relatively higher in all other tissues. Activity can also be observed in the meninges. The tumor 1/meninges and tumor 2/meninges [18F]FHBG activity ratio in this patient was 1.75 and 1.57, respectively. Whereas the average resected tumor site/meninges and intact tumor site to meninges [18F]FHBG activity ratio in control patients was 0.86 and 0.44, respectively.
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F1
Figure 1 shows enhanced [18F]FHBG accumulation within the tumor resection site, where CTLs had been infused.