PMC7543888_01
Pathological examination of lymph node and breast surgical specimen.. A) Section of axillary lymph node showing germinal centers in reactive exhaustion and mild expansion of T-dependent paracortical zone, consistent with benign reactive lymphadenitis post viral infection (Hematoxylin-Eosin staining, 2x; 10x in the insert).. B) Section of breast tumor morphologically consistent with invasive ductal carcinoma, NST according to WHO Classification, grade 2 according to the Elston-Ellis modification of the Scarff-Bloom-Richardson (SBR) grading system (Hematoxylin-Eosin staining, 2x; 10x in the insert).. C) Immunostaining showing Estrogen Receptor expression in neoplastic cells (clone SP1, Ventana Roche, 10x).. D) Immunostaining showing Progesterone Receptor expression in neoplastic cells (clone 16, Leica, 10x).. E) Proliferative index evaluated by Ki-67 immunostaining (clone MM1, Leica, 10x).. F) Immunostaining for evaluation of Her2 expression, showing mild cytoplasmic reactivity and absence of membranous staining in neoplastic cells, classified as score 0 (clone CB11, Leica, 10x).
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Immunohistochemical staining revealed strongly and diffusely ER and PR positive in tumor cells: <95% and 40% respectively (Fig. 1B-D) HER2 was negative and Ki67 was 30% (Fig. 1E and F). Sentinel lymph node presented a micrometastasis of 0.3 mm and showed germinal centers in reactive exhaustion and mild expansion of T-dependent paracortical zone, consistent with benign reactive lymphadenitis as a result of the viral infection (Hematoxylin-Eosin staining, 2x; 10x in the insert) (Fig. 1A).