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Overview of the snoRNA expression region on chromosome 15. Summary of the Prader-Willi syndrome region on chromosome 15q11.2-q13. C/D box snoRNAs are indicated as SNORD and their gene number.. Notes: The SNORD116 cluster consists of 24 expression units and the SNORD115 cluster of 47 expression units and is indicated in yellow. IPW: imprinted in Prader-Willi. Microdeletion of snoRNA expressing units that cause PWS are indicated by arrows. Patient 1, Patient 2, Patient 3. The sequences for the snoRNA probes were gggtcaatgatgagaaccttatattgtcctgaagagaggtgatgacttaaaaatcatgctcaataggattacgctgaggccc for SNORD115-11 and GGACCTCAGTTCCGATGAGAACGACGGTATGAGTTCTCACTCATTTTGTTC AGCTTTTCCAAGGAATGTTTTTATGGGGGACTCATCATCGATCCA for SNORD116-3.
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As shown in Figure 1D, patient 4876 had expression at SNRPN that was more than any of the 3 control brain samples and a very robust expression at SNORD116.
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Overview of the snoRNA expression region on chromosome 15. RNase protection analysis of 10 mug frontal cortex RNA from PWS patients and control patients.. Notes: As a probe, uniformly 32P-labeled antisense probes were used. The RNA marker is 150, 100, 90, 80, 70, 60, 50, 40 and 30 nt. The cartoon on the right shows the localization of the riboprobes and the outline of an RNase protection assay.
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Next, the expression of small nuclear ribonucleoprotein polypeptide N (SNRPN) that hosts SNORD115 and SNORD116 in their 3' UTR was analyzed (Fig. 2).
PMC3656643_05
Overview of the snoRNA expression region on chromosome 15. Summary of the Prader-Willi syndrome region on chromosome 15q11.2-q13. C/D box snoRNAs are indicated as SNORD and their gene number.. Notes: The SNORD116 cluster consists of 24 expression units and the SNORD115 cluster of 47 expression units and is indicated in yellow. IPW: imprinted in Prader-Willi. Microdeletion of snoRNA expressing units that cause PWS are indicated by arrows. Patient 1, Patient 2, Patient 3. The sequences for the snoRNA probes were gggtcaatgatgagaaccttatattgtcctgaagagaggtgatgacttaaaaatcatgctcaataggattacgctgaggccc for SNORD115-11 and GGACCTCAGTTCCGATGAGAACGACGGTATGAGTTCTCACTCATTTTGTTC AGCTTTTCCAAGGAATGTTTTTATGGGGGACTCATCATCGATCCA for SNORD116-3.
ccrep-6-2013-079f1a.jpg
PMC3656643_05_ccrep-6-2013-079f1a.jpg
f1a-ccrep-6-2013-079
As shown in Figure 1D, patient 4876 had expression at SNRPN that was more than any of the 3 control brain samples and a very robust expression at SNORD116.
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