PMC8100418_01
PMC8100418_02
PMC8100418_03
(a) Computed tomography scan of the abdomen showing multiple liver abscesses. (b) Universal container with turbid cerebrospinal fluid. (c) Blood agar plate with Klebsiella pneumoniae culture. (d) Genome distance tree comparing the clinical hypervirulent K. pneumoniae isolate with 10 reference K. pneumoniae isolates produced using Mash.
CRIID2021-6638780.001.jpg
PMC8100418_04_CRIID2021-6638780.001.jpg
fig1
The CSF was turbid and yellow (Figure 1(b)), with a very high opening pressure of >57 centimetres of CSF (above the maximum recordable level of the manometer). The blood culture isolate was hypermucoid (Figure 1(c)) and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as K. pneumoniae. Distance-based phylogenetic analysis showed the novel isolate clustered with the ST23 (sequence type) K1 hypervirulent clinical isolates SGH10 and NTUH-K2044 and was closest to SGH10 which is representative of the dominant globally distributed liver abscess-associated subclade CG23-I (Figure 1(d)).
PMC8100418_04
(a) Computed tomography scan of the abdomen showing multiple liver abscesses. (b) Universal container with turbid cerebrospinal fluid. (c) Blood agar plate with Klebsiella pneumoniae culture. (d) Genome distance tree comparing the clinical hypervirulent K. pneumoniae isolate with 10 reference K. pneumoniae isolates produced using Mash.
CRIID2021-6638780.001.jpg
PMC8100418_04_CRIID2021-6638780.001.jpg
fig1
The CSF was turbid and yellow (Figure 1(b)), with a very high opening pressure of >57 centimetres of CSF (above the maximum recordable level of the manometer). The blood culture isolate was hypermucoid (Figure 1(c)) and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as K. pneumoniae. Distance-based phylogenetic analysis showed the novel isolate clustered with the ST23 (sequence type) K1 hypervirulent clinical isolates SGH10 and NTUH-K2044 and was closest to SGH10 which is representative of the dominant globally distributed liver abscess-associated subclade CG23-I (Figure 1(d)).