PMC2996005_01
Papillary luminal proliferation with intraepithelial crypts filled with mucin
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Microscopically, the specimen showed a nonkeratinized epithelial lining with areas of papillary growth enclosed with intraepithelial crypts that were either empty or filled with mucin [Figure 1].
Epithelial plaque showing clear cells and epidermoid-like cells
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Many mucous cells, clear cells and few cells resembling that of epidermoid cells were also observed [Figure 2].
Epithelial sphere or epithelial plaque showing mucous cells
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Focal areas depict epithelial thickening or plaque formation [Figure 3].
Cystic spaces in capsule filled with mucin
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Many cystic spaces filled with mucin were evident in the fibrous connective tissue capsule [Figure 4].
Mucicarmine stain: Mucous cells and mucous pooling showing positivity
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The presence of mucin was confirmed by mucicarmine staining [Figure 5].
Alcian blue stain (acetate buffer, pH 2.5) showing positivity for mucous cells and mucous pooling
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Mucous cells and mucous pooling showed positivity to alcian blue and PAS, suggestive of acidic mucin [Figures 6 and 7].
PAS stain: Clear cells showing positivity (without diastase)
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Mucous cells and mucous pooling showed positivity to alcian blue and PAS, suggestive of acidic mucin [Figures 6 and 7]. Clear cells were positive for PAS without diastase and negative for PAS with diastase, indicating their content of glycogen [Figures 7 and 8].
PAS stain: Clear cells becoming empty again after using diastase (indicating the presence of glycogen)
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Clear cells were positive for PAS without diastase and negative for PAS with diastase, indicating their content of glycogen [Figures 7 and 8].