PMC9770126_01
Pathological study of vaginal tumor. Histologic examination shows (A) a bluish submucosal proliferation growing in a solid fashion close to the resection margins. Molding and smudging phenomena, especially in the periphery of the lesion and around the vessels, are a distinctive feature (hematoxylin-eosin [H&E], 2x). Diffuse areas and (B) well-defined nests are observed (H&E, 20x). C, A relatively monotonous population of small round cells with finely stippled "salt and pepper" nuclear chromatin, inconspicuous nucleoli, and scant cytoplasm constitutes the lesion. A thin delicate fibrovascular stroma is also noted (H&E, 40x). D, Immunohistochemistry reveals the neuroendocrine nature of the tumor cells with a diffuse membrane expression of synaptophysin. E, The neoplasm is also positive for chromogranin A with a perinuclear dot-like pattern. F, An intense nuclear p16 expression is identified. Cytokeratin positivity was demonstrated with (G) CAM.5, CK7, and CK20 stains.
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F1
Type 18 human papillomavirus (HPV) was detected in biopsy specimen, and an intense nuclear p16 expression was observed (see Figure 1).