PMC3919847_01
Non-polarized dermatoscopic image of a lesion on the anterior left shoulder of a 27-year-old woman. There is a pattern of apparently exophytic brown clods centrally, surrounded by a zone of structureless brown with a pattern of small brown clods peripherally. There are some eccentric foci of darker brown pigment on the right side of and to the right of the larger clods. [Copyright:  2014 Cohen et al.]
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Prior to excision biopsy, dermatoscopic images were obtained in both polarizing and non-polarizing mode (Figures 1-3) with a Canfield DermScope (Canfield Scientific Inc.
Polarized dermatoscopic image of the same lesion as displayed in Figure 1. In this image a millimeter scale reveals that the dimensions of the dark brown lesion are 5 x 4 mm. White lines that are clearly whiter than the patient's skin color are present over the center of the lesion and demarcate the clods seen with non-polarizing dermatoscopy (Figure 1). A pattern of small brown clods surrounds the lesion in some parts, extending to the borders of the image. Hairs are seen on the lesion peripheral to, but not over, the pattern of white lines. [Copyright:  2014 Cohen et al.]
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Prior to excision biopsy, dermatoscopic images were obtained in both polarizing and non-polarizing mode (Figures 1-3) with a Canfield DermScope (Canfield Scientific Inc.
A couplet of (A) non-polarizing dermatoscopy and (B) polarizing dermatoscopy of the lesion displayed in Figures 1 and 2 revealing the correlation between the polarizing-specific white lines in B and the large brown clods in A. The smaller brown clods surrounding the lesion are not separated by white lines, but by skin-colored lines. Hairs are seen in image B, but they are not present on the central part of the lesion where the polarizing-specific white lines are seen. [Copyright:  2014 Cohen et al.]
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Prior to excision biopsy, dermatoscopic images were obtained in both polarizing and non-polarizing mode (Figures 1-3) with a Canfield DermScope (Canfield Scientific Inc.
Low power hematoxylin and eosin image (40x) of the central exophytic portion of the lesion shown on Figures 1-3. A neoplastic proliferation of melanocytes is present in the epidermis as single cells and discohesive nests with clefting and in the dermis predominantly as nests. The boxed area is shown at higher power in Figure 5. Scale bar, 100 muM. [Copyright:  2014 Cohen et al.]
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Dermatopathologically (Figures 4-7) this lesion displayed both architectural and cytological criteria consistent with melanoma arising in conjunction with a compound melanocytic nevus with congenital nevus features, and benign nevus cells were reported as being present in the dermis at a deeper level than the deepest observed melanoma cell.
Hematoxylin and eosin image (100x) of the boxed area in Figure 4 showing severely atypical and epithelioid melanocytes distributed both in the epidermis as markedly discohesive and irregular junctional nests, as a near confluent proliferation of single cells along the dermoepidermal junction lining a hair follicle, and in the subjacent papillary dermis (black arrows). Fibrotic bands of collagen, many orientated vertically, are seen separating nests of neoplastic melanocytes. Scale bar, 100 muM. [Copyright:  2014 Cohen et al.]
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Dermatopathologically (Figures 4-7) this lesion displayed both architectural and cytological criteria consistent with melanoma arising in conjunction with a compound melanocytic nevus with congenital nevus features, and benign nevus cells were reported as being present in the dermis at a deeper level than the deepest observed melanoma cell.
Hematoxylin and eosin image (40x) of part of the lesion shown in Figures 1-3 adjacent to the area shown in Figure 4. In addition to a neoplastic epidermal proliferation of melanocytes (boxed area upper left) there is a broad junctional and deep dermal melanocytic proliferation present with congenital nevus architecture. Arrowheads point to the dermal component. The boxed area is shown at higher power in figure 7. Scale bar, 100 muM. [Copyright:  2014 Cohen et al.]
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Dermatopathologically (Figures 4-7) this lesion displayed both architectural and cytological criteria consistent with melanoma arising in conjunction with a compound melanocytic nevus with congenital nevus features, and benign nevus cells were reported as being present in the dermis at a deeper level than the deepest observed melanoma cell.
Hematoxylin and eosin image (200x) of the boxed area in Figure 6. Irregular junctional nests (black arrows) comprised of severely atypical epithelioid melanocytes overlie dermal nests of banal and nevic appearing melanocytes (asterisks). Scale bar, 100 muM. [Copyright:  2014 Cohen et al.]
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Dermatopathologically (Figures 4-7) this lesion displayed both architectural and cytological criteria consistent with melanoma arising in conjunction with a compound melanocytic nevus with congenital nevus features, and benign nevus cells were reported as being present in the dermis at a deeper level than the deepest observed melanoma cell.