PMC5664901_01
a, b Paraffin section of Anisakis larva in a ventral subcutaneous lesion. m muscle, L lateral chord, R rennet cell, T transverse striation
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Fig1
Cross sections of the larva of the nematode Anisakis were detected in ventral subcutaneous tissue (Fig. 1a, b).
Phylogenetic analysis of Anisakis species based on COX2 sequences (532 bp). Nucleotide sequences were aligned and phylogenetic tree analysis was conducted using the Genetyx ver 11 software
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Fig2
The phylogenetic tree based on COX2 sequences analysed by Genetyx ver 11 software revealed that this specimen belongs to A. simplex s.s. (Fig. 2).  
Paraffin section of Dirofilaria species in a dorsal subcutaneous lesion
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Fig3
Cross sections of the nematode Dirofilaria species were detected in dorsal subcutaneous tissue (Fig. 3).
Phylogenetic analysis of Dirofilaria species based on 5S rRNA sequences (90 bp). Nucleotide sequences were aligned using the Genetyx ver 11 and CLC Sequence Viewer 6 software. Phylogenetic analyses were conducted using the Mega 6 software. Genetic relationships were inferred by the neighbor-joining (NJ) method. D1 and D2 means Dirofilaria ursi worms collected from Japanese bears in Gifu Prefecture in the middle part of Japan
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Fig4
The phylogenetic tree of this D. ursi containing worms collected from Japanese black bears in Gifu (the middle part of Japan) based on 5S rRNA sequences is shown in Fig. 4.