PMC5094157_01
Case 1: H & E section of skin of left forearm after wide excision of melanoma 40x. Central area of organizing inflamed re-epithalized scar. The biopsy site at the edge where this is focal residual malignant melanoma invasive to a depth of 0.74 mm. The surrounding surface shows areas of residual radial growth phase/in situ melanoma.
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Pathology of the re-excisional biopsy showed focal residual malignant melanoma invasive to 0.74 mm with atypia and overlying epidermis with residual in situ melanoma (Fig. 1).
Case 1: H & E view of the sentinel lymph node shows portions of capsule to be cauterized with central hilar fatty replacement. Sinus histiocytes are scattered in moderate numbers in the sinusoids. Indicative of capsular nevus.
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Morphological observations of sentinel lymph nodes were consistent with capsular nevus (Fig. 2).
Case 1: S-100 immunostain shows 0.4 mm focus of small bland appearing cells within the capsule of sentinel lymph node that are not discretely identifiable on H & E stained section levels but appear to have small round nuclei.
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Sentinel lymph node was positive for Melan-A, S100 (Fig. 3), and MART-1 (Fig. 4).
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Case 2: H & E of sentinel lymph node shows bland melanocytes within the capsule with uncertain significance.
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One sentinel node demonstrated bland melanocytes within the capsule of uncertain significance and could represent benign nevus cells but identification was not certain (Fig. 5).
Case 2: S100 immunostain of sentinel lymph node capsule showed to be positive.
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These aggregates were of a lesser degree of atypia compared to primary tumor but were S100 (Fig. 6) and MART-1 (Fig. 7) positive.