PMC3469179_01
Lane A, DNA size marker 50 bp; Lane B, Plasmodium vivax (positive control, 499 bp); Lanes C and E Plasmodium falciparum (positive control, 395 bp); Lane D, Plasmodium vivax obtained from the peripheral blood smear preparation of the patient and Lanes F, negative controls
IJPA-7-099-g001.jpg
PMC3469179_01_IJPA-7-099-g001.jpg
F0001
After 2 weeks, Semi-nested multiplex PCR based on the sequence of the small subunit ribosomal RNA (ssrRNA) gene was performed according to the method of Edoh et al. and Rubio et al., that confirmed the presence of P. vivax in blood smear of the patient (Fig. 1), also the amplicon was sequenced and recorded in the GenBank under the following Accession Number: JN084167.