PMC9476239_01
The morphology of the specimen from biopsy of retroperineum mass shows a picture of proliferation of neoplastic cells in sheet pattern with adjacent loose stromal tissue having inflammatory cells infiltration, including lymphocytes and eosinophils (a and b).
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Subsequent pathology interpretation of computed tomography (CT)-guided biopsy of retroperitoneum mass was spermatocytic seminoma according to morphologic pictures (Figure 1) and the expression of CD30 and CD117 immunohistochemically.
Morphologically, the tumor cells of ovary show diffuse infiltration of mast cells with spindle shapes (a and b). The tumor cells of retroperitoneum show diffuse infiltration of atypical round-shaped epithelioid tumor cells, with the majority showing prominent nucleoli and eosinophilic to amphophilic cytoplasm, while scattered cells have hyperchromatic nuclei without nucleoli located peripherally resembling mast cells (c and d).
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Mastocytosis in ovarian tissue was reported first (Figure 2a and b). Morphologically, there were diffuse infiltrations of atypical round-shaped epithelioid tumor cells, with the majority showing prominent nucleoli and eosinophilic to amphophilic cytoplasm, while scattered cells had hyperchromatic nuclei without nucleoli located peripherally resembling mast cells (Figure 2c and d).
Immunohistochemically, the tumor cells of retroperitoneal specimens show strong positivity for CD30 (a), CD117 (b), and strong and diffuse positivity for mast cell tryptase (c). The tumor cells of ovarian specimens show diffuse expression of mast cell tryptase (d).
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Immunohistochemistry staining (IHC) showed diffuse positivity for CD117, CD30, CD33, CD43, tryptase and focal positive for CD25, CD68 and negative for CD2, myeloperoxidase and lysozyme (Figure 3).