PMC4340331_01
Molecular detection of
Plasmodium ovale
in blood samples from patient by the amplification of 18S gene. Nested PCR with Plasmodium genus-conserved primers was followed by P. ovale specific primers. PCR products were separated by electrophoresis through a 2.0% agarose gel stained with ethidium bromide and flanked by a 50 bp DNA ladder (Novagen) as a size marker. Lane MM: Molecular marker 50 bp. Lane TBF: Patient's thin blood film. LaneTBF-2: Patient's thin blood film (duplicate). Lane WB: Patient's whole blood. Lane
Pf
:
Plasmodium falciparum control. Lane
Pv
:
Plasmodium vivax control. Lane
Pm
:
Plasmodium malariae control. Lane Po:
Plasmodium ovale positive control. Lane C-: Negative control.
40779_2015_30_Fig1_HTML.jpg
PMC4340331_01_40779_2015_30_Fig1_HTML.jpg
Fig1
A P. ovale mono-infection was identified on both the blood film and whole blood of the patient using a Plasmodium genus-specific nested PCR (Figure 1) and a SYBR Green-based quantitative real-time PCR.
Plasmodium ovale
parasites (initially diagnosed as
P. vivax
) shown in different stages in the blood smear of the patient at time of presentation (Giemsa Stain, X 1000). A and C: ring-form trophozoites. B and D: gametocytes.
40779_2015_30_Fig2_HTML.jpg
PMC4340331_01_40779_2015_30_Fig2_HTML.jpg
Fig2
The blood film was re-examined to identify the morphological features of this parasite (Figure 2).