PMC6839572_01
The radiological and pathological examinations of the patient. (A) Computed tomographic angiography (CTA) of the coronary artery revealed a low-density mass (white arrow) in the right atrium. (B) Microscopic examination showed diffuse infiltration by large lymphoid cells with round or oval nuclei and an altered nuclear-cytoplasmic ratio (original magnificationx200). (C-I) Immunohistochemical staining of the lymphoid cells (original magnificationx200). (C) Positive staining for CD20. (D) Negative staining for CD10. (E) Positive staining for Bcl-6. (F) Positive staining for Mum1. (G) Positive staining for Bcl-2. (H) Positive staining for MYC. (I) The Ki67 staining demonstrating a 80% proliferative rate.
OTT-12-9085-g0001.jpg
PMC6839572_01_OTT-12-9085-g0001.jpg
F0001
The computed tomographic angiography (CTA) of the coronary artery demonstrated a low-density bulk in the right atrium with dimensions of 70 mm X 52 mm infiltrating into both the superior vena cava (SVC) and inferior vena cava (IVC), oppressing the pulmonary vein (Figure 1). The Ki67 index was 80% (Figure 1). TP53/CSP17 Probe kit (17p13.1/17p11.1-q11) (Jinpujia medical technology Company Limited, Beijing, China), the deletion of 17p13.1 containing TP53 gene was confirmed by Fluorescence in situ hybridization (FISH) (Figure S1) which was in accordance with SNP array. The patient received 6 courses of chemotherapy (rituximab, cyclophosphamide, doxorubicin, vincristine, prednisone, R-CHOP regimen) after surgery, achieved complete remission as evaluated by PET/CT and showed normal cardiac function by echocardiography.
Copy number variations in the present case of primary cardiac diffuse large B cell lymphoma. Four cryptic chromosomal aberrations (<=5 Mb), all of which were chromosomal loss, were recognized at 12q24.31, 17p13.1, 19p13.3 and 19q13.42q13.43 and harbored interesting genes.
OTT-12-9085-g0002.jpg
PMC6839572_01_OTT-12-9085-g0002.jpg
F0002
Santa Clara, CA, USA) according to the manufacturer's protocols. Copy number analysis was estimated utilizing the Chromosome Analysis Suite 3.0 (ChAS 3.0) software, which enabled us to view and summarize chromosomal aberrations across the genome including copy number gain or loss, or loss of heterozygosity. In total, 19 CNVs involving 13 chromosomes (1, 3, 5, 6, 7, 11, 12, 15, 17, 18, 19, X, and Y), including 11 chromosomal gain and eight chromosomal loss CNVs, and four copy-neutral loss of heterozygosity (CN-LOH) lesions were identified by SNP array karyotyping (Figure 2). Sixteen large chromosomal abnormalities with sizes greater than 5 Mb were observed with multiple chromosome involvement, for example, 1q+, 3p+, 3q+ 5p+, 6p-, 6q-, 7q+, +11, 15q-, 17q+, +18, 19q+, Xq+ and -Y.