PMC10313112_01
Bone marrow sections of the four patients. Case 1: The red arrow showed erythroblast proliferation, supporting the diagnosis of polycythemia vera. Case 2: The blue arrow showed hyperlobulated megakaryocyte, supporting the diagnosis of essential thrombocythemia. Case 3 and Case 4: The green arrow showed megakaryocytes with atypical and bare nucleus, supporting the diagnosis of primary myelofibrosis.
fgene-14-1198834-g001.jpg
PMC10313112_04_fgene-14-1198834-g001.jpg
F1
Peripheral blood smears and bone marrow tissues revealed the diagnosis the MPN (Table 2; Figure 1).
Genomic landscape of myeloproliferative neoplasms. (A) Driver gene mutation frequencies. (B-D) MPL, JAK2 and CALR gene structures. The red dots represent hotspot mutations in COSMIC. The gray arrows represent rare/novel mutations of the cases (MPL p.W515_Q516del, JAK2 p.H538_K539delinsQL, CALR p.E380Rfs*51).
fgene-14-1198834-g002.jpg
PMC10313112_03_fgene-14-1198834-g002.jpg
F2
Targeted NGS was performed, and the MPL p.W515_Q516del (Figure 2) mutation with a VAF of 68.9% was identified.
PMC10313112_02
Bone marrow sections of the four patients. Case 1: The red arrow showed erythroblast proliferation, supporting the diagnosis of polycythemia vera. Case 2: The blue arrow showed hyperlobulated megakaryocyte, supporting the diagnosis of essential thrombocythemia. Case 3 and Case 4: The green arrow showed megakaryocytes with atypical and bare nucleus, supporting the diagnosis of primary myelofibrosis.
fgene-14-1198834-g001.jpg
PMC10313112_04_fgene-14-1198834-g001.jpg
F1
Peripheral blood smears and bone marrow tissues revealed the diagnosis the MPN (Table 2; Figure 1).
Genomic landscape of myeloproliferative neoplasms. (A) Driver gene mutation frequencies. (B-D) MPL, JAK2 and CALR gene structures. The red dots represent hotspot mutations in COSMIC. The gray arrows represent rare/novel mutations of the cases (MPL p.W515_Q516del, JAK2 p.H538_K539delinsQL, CALR p.E380Rfs*51).
fgene-14-1198834-g002.jpg
PMC10313112_03_fgene-14-1198834-g002.jpg
F2
Targeted NGS was performed, and the MPL p.W515_Q516del (Figure 2) mutation with a VAF of 68.9% was identified.
PMC10313112_03
Bone marrow sections of the four patients. Case 1: The red arrow showed erythroblast proliferation, supporting the diagnosis of polycythemia vera. Case 2: The blue arrow showed hyperlobulated megakaryocyte, supporting the diagnosis of essential thrombocythemia. Case 3 and Case 4: The green arrow showed megakaryocytes with atypical and bare nucleus, supporting the diagnosis of primary myelofibrosis.
fgene-14-1198834-g001.jpg
PMC10313112_04_fgene-14-1198834-g001.jpg
F1
Peripheral blood smears and bone marrow tissues revealed the diagnosis the MPN (Table 2; Figure 1).
Genomic landscape of myeloproliferative neoplasms. (A) Driver gene mutation frequencies. (B-D) MPL, JAK2 and CALR gene structures. The red dots represent hotspot mutations in COSMIC. The gray arrows represent rare/novel mutations of the cases (MPL p.W515_Q516del, JAK2 p.H538_K539delinsQL, CALR p.E380Rfs*51).
fgene-14-1198834-g002.jpg
PMC10313112_03_fgene-14-1198834-g002.jpg
F2
Targeted NGS was performed, and the MPL p.W515_Q516del (Figure 2) mutation with a VAF of 68.9% was identified.
PMC10313112_04
Bone marrow sections of the four patients. Case 1: The red arrow showed erythroblast proliferation, supporting the diagnosis of polycythemia vera. Case 2: The blue arrow showed hyperlobulated megakaryocyte, supporting the diagnosis of essential thrombocythemia. Case 3 and Case 4: The green arrow showed megakaryocytes with atypical and bare nucleus, supporting the diagnosis of primary myelofibrosis.
fgene-14-1198834-g001.jpg
PMC10313112_04_fgene-14-1198834-g001.jpg
F1
Peripheral blood smears and bone marrow tissues revealed the diagnosis the MPN (Table 2; Figure 1).