PMC9492991_01
Fluorescence in situ hybridization (FISH) analysis of Case 1 on interphase with an LSI ETV6/RUNX1 ES dual color probe. RUNX1 signals are in red, and ETV6 signals are green. (A) Cells at disease onset. (B) Cell at relapse.
fped-10-960126-g001.jpg
PMC9492991_01_fped-10-960126-g001.jpg
F1
However, FISH revealed 5-12 signals of RUNX1 in 68% of interphase cells (Figure 1A). FISH analysis revealed no ETV/RUNX1 fusion signals and five signals for the RUNX1 probe in 96% of the analyzed nuclei (Figure 1B).
PMC9492991_02
FISH analysis of Case 2 on interphase and metaphase with an LSI ETV6/RUNX1 ES dual color probe. RUNX1 signals are in red, and ETV6 signals are green. (A) An interphase cell at relapse. (B) A metaphase cell at relapse.
fped-10-960126-g002.jpg
PMC9492991_02_fped-10-960126-g002.jpg
F2
G-banded chromosome analysis of the BM sample revealed a female karyotype 46, XX, and FISH analysis revealed no ETV/RUNX1 fusion signals and 5-10 signals for the RUNX1 probe in 83% of the analyzed nuclei (Figure 2).