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A, B. Morphology analysis of the BM cells reveals a population of 27% medium sized blast cells that present irregular nuclear outlines, fine chromatin and reduced agranular basophilic cytoplasm.
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U/L; reference range 120 - 246 U/L), a marker of tumor cell lysis. Under these circumstances, BM aspiration was performed and morphology analysis identified 27% medium sized blast cells with irregular nuclear outline, lax chromatin and reduced basophilic cytoplasm (Figures 1A and 1B).
Flow cytometry was performed on the BM cells. The blastic plasmacytoid dendritic cell population is highlighted in orange. The blasts were intermediate positive for CD45 (a); partially positive for CD117 and CD7 (b, c); positive for CD4, CD36, CD56, CD123, HLA-DR and NG2 (d, e, f, g, h, i) and negative for CD94 (f), CD34 (j), cyCD3 and cyCD79a (k) and cyMPO (l).
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The precursor cell marker CD34 was absent and lineage markers cyMPO, cyCD79a, cyCD3 and CD94 were also negative (Figure 2).