PMC5892108_01
Obtained germinal vesicle oocyte after denudation
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F1
After 36 h, one oocyte was collected which was found to be immature [Figure 1] (germinal vesicle stage) when denuded.
MII oocyte after in vitro maturation
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F2
The matured oocyte [Figure 2] (metaphase II) was injected with the husband sperm after processing the semen by swim-up method.
Two pronuclei stage
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F3
Two pronuclei [Figure 3] were seen 18 h post-ICSI and a grade 1 embryo [Figure 4] was generated on day 3.
Transferred day 3 embryo
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F4
Two pronuclei [Figure 3] were seen 18 h post-ICSI and a grade 1 embryo [Figure 4] was generated on day 3.