PMC9416326_01
A 2D (top) and M-mode (bottom) imaging of parasternal long-axis echocardiogram showing pericardial tamponade. Bright yellow arrows show dark echo-free signal from pericardial fluid with left ventricular contraction in both end-diastolic and end-systolic.
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A cardiac ultrasound exam revealed a significantly enlarging pericardial effusion (Figure 1).
Representative image string test. Strings >5mm indicates the hypermucoviscous phenotype of the KP strain.
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All isolates demonstrated hypermucoviscosity as shown by string length >5 mm in the string test (Figure 2).
Serum survival assay of three isolates (pericardial, blood and ascites). (A) Colony-forming units (CFUs) recovery after co-incubation of the isolates with pooled normal serum on blood plates for 1, 2 and 3 hours. Images are representative of two parallel samples of each isolate. (B) Mean viable CFU counts of the replicates at each time point.
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As shown in Figure 3, the colony-forming unit (CFU) recovery of all isolates was inhibited by the human serum time-dependently. After three hours of incubation, barely any CFU colony was visible in two samples and no colony growth was observed by the next day (Figure 3A and B), indicating a susceptibility of the KP strain to normal human serum.