PMC5142052_01
Bark and leaf characters assessed. Different bark types: (A) 'smooth', typical for R. irroratum, (B) 'intermediate', (C) deeply ridged = 'deep', typical for R. delavayi. (D) Different indumentum types (from left to right): 'glabrous'/R. irroratum, 'intermediate', 'thick'/R. delavayi; measured leaf characters.
plw070f1p.jpg
PMC5142052_01_plw070f1p.jpg
plw070-F1
The characters measured are illustrated in Figs 1-3. The means of the two parental species were chosen as end points of this index; because the two means were not exactly mirrored with respect to 0 [see SUPPORTING INFORMATION:Figure S1], the average of the two means were used as an adjustment to retain symmetry.
Corolla characters assessed. Nectar pouches and corolla colour: (A) 'deep-red' corolla, one 'dominant' nectar pouch:the main nectar pouch in the middle is distinctly larger, and has a different colour; (B) 'deep-red' corolla, 'different' nectar pouches:the middle nectar pouch is perceptibly larger, and the pouches next to the main one are larger then the others, and all are distinctly coloured; (C) 'pink' corolla, 'different' nectar pouches; (D) 'cream' corolla, 'equal' nectar pouches:no distinct colouring, and all are the same size. 'pink-red' corolla colour is not shown, as it was indistinguishable from 'deep-red' in photographs. (E) Typical R. irroratum corolla; (F) typical R. delavayi corolla, shown are measurements of 'corolla length':measured as the length from the calyx to the tip of the main petal (compare A, middle petal), and 'corolla width':measured at the top of the corolla tube (where the petals are just still all fused). Maculation: (G) 'none':absolutely no markings visible; (H, I) 'scarce':some light spots visible on fewer than three petals; (J) 'few':spots clearly visible on three petals; (K, L) 'many':spots clearly visible on all petals.
plw070f2p.jpg
PMC5142052_01_plw070f2p.jpg
plw070-F2
The characters measured are illustrated in Figs 1-3.
Stamen, style and ovary characters. (A) Hairs on the base of the stamens 'present'; Style and ovary: (B) 'red' style, with no hairs on style:style hairs 'NA', ovary hairs are 'dense':ovary is not visible through the hairs:and glands are 'absent'; (C) 'red-green' style, that is 'hairy', ovary hairs are 'dense', and glands are 'absent'; (D) 'green' style, with 'glandular' hairs nearly to the top, ovary is 'hairy':green ovary visible, and ovary glands are 'present'. (E) Close-up of the ovary in D, glandular hairs with red heads are clearly visible. (F) Measurements of 'style length':from the top of the ovary to the top of the stigma, and 'distance anther stigma':the distance from the top of the stigma to the closest anther, when projected onto the style. (G, H) Stigmas; the measurement 'stigma width' was always the widest point, as the shape is oval. (B, G) Typical of R. delavayi; (D, E, H) typical of R. irroratum.
plw070f3p.jpg
PMC5142052_01_plw070f3p.jpg
plw070-F3
The characters measured are illustrated in Figs 1-3.
Map of the sampling area in Baili. Indicated are the positions of all individuals that were measured, based on recorded GPS coordinates.
plw070f4p.jpg
PMC5142052_01_plw070f4p.jpg
plw070-F4
We aimed to cover the whole population, and plants of the same species were sampled at least 10 m apart to avoid kinship, although in some cases intermediate individuals were sampled closer to one individual belonging to one of the two species (Fig. 4).