PMC7845664_01
Key histological images of preneoplastic lesions and invasive cervical cancer. (a) CIN I associated to HPV infection (koilocytes). (b) CIN II-III associated to HPV infection (koilocytes). (c) Invasive squamous cell carcinoma.
CRIOG2020-6806857.001.jpg
PMC7845664_01_CRIOG2020-6806857.001.jpg
fig1
II-III:with glandular extension (Figure 1(b)) and low-grade squamous intraepithelial lesion:CIN I, associated with HPV infection (Figure 1(a)). In the 2002 slides, invasive squamous cell carcinoma was confirmed as invasive carcinoma type large squamous cell carcinoma, nonkeratinizing, infiltrating, and moderately differentiated of the cervix (Figure 1(c)).
Protein expressions in preneoplastic lesions, invasive cancer, and controls.
CRIOG2020-6806857.002.jpg
PMC7845664_01_CRIOG2020-6806857.002.jpg
fig2
Strong expressions of CAIX, GLUT1, and hTERT in preneoplastic lesions were observed while a weak expression of GLUT1 and Survivin was observed in cancer samples (Figure 2).
Allele-specific PCR-Arg72Pro. Lanes: 1: molecular weight marker; 2-4: beta-globina amplicon 209 bp: S1 (paraffin block sample:1986), S2 (paraffin block sample:2002), H (HeLa); 5-7: Arg72Pro P53 amplicons (178 bp Pro, 136 bp Arg): S1, S2, and H (HeLa: heterozygous genotype Arg72Pro).
CRIOG2020-6806857.003.jpg
PMC7845664_01_CRIOG2020-6806857.003.jpg
fig3
The study of the TP53 polymorphism revealed an arginine/proline genotype in both samples (from the preinvasive in 1986 and the invasive cancer in 2002). The amplification signal by PCR was more intense for proline than for arginine (Figure 3).