PMC4323713_01
Indurated erythematous-ferruginous plaque with central atrophic scars (from
previous biopsies) on the lateral side of the right leg
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A 63-year-old female patient had an indurated, infiltrated erythematous-ferruginous plaque with central atrophic scars (from previous biopsies) on her right leg (Figure 1).
Ulcer with irregular borders and center with areas of necrosis and fibrin on the
lateral side of the right leg
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We repeated the biopsy and the lesion progressed to a 6-cm ulcer with irregular borders and fibrino-necrotic areas (Figure 2).
H.E. Areas of granulation tissue with foci of lymphocytic inflammatory infiltrate
and associated fibrosis are seen in the dermis
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The anatomicopathological study showed histiocytes and multinucleated giant cells (Figure 3).
Electrophoresis in agarose gel of the PCR products. Lane M, molecular weight
marker (100 bp ladder); lane 1, PCR product obtained from the patient s sample
(positive for M. tuberculosis). Lane 2, positive control (M. tuberculosis); lane
3, negative control
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TB1 (primers: TB1 (5'-CCTGCGAGCGTAGGCGTCGG-3') and TB2 (5'-CTCGTCCAGCGCCGCTTCGG-3')), which amplify a fragment of 123 base-pairs (bp) of the IS6110 sequence (Figure 4). The treatment was performed with pyrazinamide, rifampicin, isoniazid and ethambutol.