PMC5067888_01
FISH on the urine cytology sample with probes for the X chromosome centromere (red) and the Y chromosome heterochromatic region (green) (Abbott Molecular, Downers Grove, IL); large malignant urothelial carcinoma cell with four X chromosome signals and a normal male cells with one X and one Y chromosome signal
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Fig1
Urine cytology was positive for malignant cells that were shown to be of female origin by fluorescence in situ hybridization (FISH) with 2-8 copies of the X chromosome and no copy of the Y chromosome (Fig. 1).
FISH on the surgical specimen post resection with probes for X chromosome centromere (red) and the Y chromosome heterochromatic region (green) (Abbott Molecular, Downers Grove, IL) showing only X chromosome signals consistent with female donor origin of the urothelial cancer
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Fig2
FISH for the X and Y chromosomes performed on the urothelial carcinoma from the explanted transplant kidney confirmed that the malignant cells were of female donor origin (Fig. 2).