PMC3420651_01
PMC3420651_02
Light microscopy image (a) and TOF-SIMS image (b) of the tissue section from the PTU pretreated patient. The green in the TOF-SIMS image represents the signal from iodine and the red marks PO3
- (signal from cell membranes). Iodine signals were mainly found within the follicle lumina but also in some areas outside the follicles, presumably in the thyrocytes. The iodine concentration was inhomogeneously distributed within some follicle lumina.
CRIM.ENDOCRINOLOGY2012-842357.001.jpg
PMC3420651_02_CRIM.ENDOCRINOLOGY2012-842357.001.jpg
fig1
 The images in Figures 1, 2, and 3 show typical light micrographs and TOF-SIMS ion images for the samples from the two patients. TOF-SIMS images showed that iodine was present within the follicle lumina (Figures 1 and 3). There was variation in iodine concentration between (Figure 3(b)) and within (Figure 1(b)) some follicle lumina. The sample from Patient 1 also showed iodine signals outside the follicle lumina. The thyrocytes in the PTU-treated patient appeared more similar to the cell shape found in subjects with healthy thyroids (Figure 1(a)).
TOF-SIMS image of a second tissue section from the PTU pretreated patient. The green in the TOF-SIMS image represents the signal from iodine and the red marks PO3
- (signal from cell membranes). Iodine signals were located within the follicle lumina, but also within thyrocytes.
CRIM.ENDOCRINOLOGY2012-842357.002.jpg
PMC3420651_02_CRIM.ENDOCRINOLOGY2012-842357.002.jpg
fig2
 The images in Figures 1, 2, and 3 show typical light micrographs and TOF-SIMS ion images for the samples from the two patients. Magnification of such an area revealed the iodine signals were colocalized with the thyrocytes (Figure 2).
Light microscopy image (a) and TOF-SIMS image (b) of the tissue section from the patient pretreated with stable iodine. The green in the TOF-SIMS image represents the signal from iodine and the red marks PO3
- (signal from cell membranes). Iodine was located within the follicle lumina. The differences in iodine signal intensity indicate a difference in iodine concentration between the follicle lumina.
CRIM.ENDOCRINOLOGY2012-842357.003.jpg
PMC3420651_02_CRIM.ENDOCRINOLOGY2012-842357.003.jpg
fig3
 The images in Figures 1, 2, and 3 show typical light micrographs and TOF-SIMS ion images for the samples from the two patients. TOF-SIMS images showed that iodine was present within the follicle lumina (Figures 1 and 3). There was variation in iodine concentration between (Figure 3(b)) and within (Figure 1(b)) some follicle lumina. The sample from Patient 1 also showed iodine signals outside the follicle lumina. In tissue from Patient 2, the patient pretreated with stable iodine, the thyrocytes were enlarged, with a cubical shape corresponding to hyperfunctioning thyrocytes (Figure 3(a)).