PMC3564823_01
The cut surface was solid gray in fish appearance with haemorrhage and necrosis in the central area.
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PMC3564823_01_1746-1596-8-14-1.jpg
F1
The cut surface was solid gray in fish appearance with haemorrhage and necrosis in the central area (Figure 1).
A. The tumour was mainly composed of abundant spindle cells. The tumour was absent of glands except that one elongated and narrow gland was displayed here after widely sectioned. (HE x 50) B. The tumour cells were gathered and abundant, whereas collagen was relatively rare. (HE x 100) C. Collagen bundles among the tumour cells were rather ample. (HE x 100) D. The tumour cells displayed inconspicuous eosinophilic or amphophilic cytoplasm, and striking and vesicular nuclei with coarse chromatin or obvious nucleoli. Mitoses were easily found. (HE x 400) E. CD10 was strongly and diffusely positive. (MaxVision method x200) F. EGFR was strongly and diffusely positive. (MaxVision method x 200).
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PMC3564823_01_1746-1596-8-14-2.jpg
F2
The spindle cells were arranged in fascicles, occasionally in storiform pattern (Figure 2A). The collagen in most areas was scarce and inconspicuous (Figure 2B), whereas in some focal regions was rather ample (Figure 2C). The nuclei were striking, polymorphic and vesicular with coarse chromatin or obvious nucleoli (Figure 2D). After sectioned widely, only one elongated and narrow gland was found (Figure 2A). The immunostaining investigation showed that CD10 (56C6, Novacastra) and vimentin (V9, Invitrogen) were positive strongly and diffusely (more than 90% of tumour cells) (Figure 2E). ER (1D5, Invitrogen), PR (1A6, Invitrogen) and AR (H7507, Invitrogen). EGFR (H11, Dako) was membrane positive strongly and diffusely (more than 90%) (Figure 2F).
The tumour cell was spindle and had the striking nucleus with irregular and distorted envelope and one significant nucleolus. Apparatuses in cytoplasm were rare and lysosomes were relatively abundant.
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PMC3564823_01_1746-1596-8-14-3.jpg
F3
The nucleus was striking with irregular and distorted envelope and one significant nucleolus (Figure 3).