PMC10115452_01
Clinical presentation: (a) N. brasiliensis, (b)
M. marinum.
1678-9946-rimtsp-65-S1678-9946202365029-gf01.jpg
PMC10115452_02_1678-9946-rimtsp-65-S1678-9946202365029-gf01.jpg
f01
A 63-year-old Caucasian female, with a medical history of ankylosing spondylitis under immunotherapy, was referred to our hospital for having a 3-month history of a rapidly enlarging painful mass with malodorous discharge and surrounding edema on her right hand (Figure 1B).
Gram stain of colonies grown on blood agar (a, c) and
Ziehl-Neelsen stain of colonies grown on Lowenstein-Jensen medium
(b, d), N. brasiliensis (a, b) and M.
marinum (c, d).
1678-9946-rimtsp-65-S1678-9946202365029-gf02.jpg
PMC10115452_02_1678-9946-rimtsp-65-S1678-9946202365029-gf02.jpg
f02
The Gram stain showed short beaded Gram-positive bacilli (Figure 2C), whereas ZN revealed acid-fast bacilli (Figure 2D).
Colonies of N. brasiliensis grown on blood (a),
Sabouraud dextrose agar plates (b) and Lowenstein-Jensen medium (c).
Colonies of M. marinum grown on blood (d),
Sabouraud dextrose agar plates (e) and Lowenstein-Jensen medium
(f).
1678-9946-rimtsp-65-S1678-9946202365029-gf03.jpg
PMC10115452_02_1678-9946-rimtsp-65-S1678-9946202365029-gf03.jpg
f03
Initial cultures on blood and Sabouraud agar plates were negative, however, growth was observed on LJ medium (Figure 3F) after 18 days of incubation. Subsequent subcultures were performed on both blood (Figure 3D) and Sabouraud dextrose agar plates (Figure 3E), resulting in the rapid growth of moist, smooth colonies that acquired a yellowish color upon prolonged culture and light exposure.
PMC10115452_02
Clinical presentation: (a) N. brasiliensis, (b)
M. marinum.
1678-9946-rimtsp-65-S1678-9946202365029-gf01.jpg
PMC10115452_02_1678-9946-rimtsp-65-S1678-9946202365029-gf01.jpg
f01
A 63-year-old Caucasian female, with a medical history of ankylosing spondylitis under immunotherapy, was referred to our hospital for having a 3-month history of a rapidly enlarging painful mass with malodorous discharge and surrounding edema on her right hand (Figure 1B).
Gram stain of colonies grown on blood agar (a, c) and
Ziehl-Neelsen stain of colonies grown on Lowenstein-Jensen medium
(b, d), N. brasiliensis (a, b) and M.
marinum (c, d).
1678-9946-rimtsp-65-S1678-9946202365029-gf02.jpg
PMC10115452_02_1678-9946-rimtsp-65-S1678-9946202365029-gf02.jpg
f02
The Gram stain showed short beaded Gram-positive bacilli (Figure 2C), whereas ZN revealed acid-fast bacilli (Figure 2D).
Colonies of N. brasiliensis grown on blood (a),
Sabouraud dextrose agar plates (b) and Lowenstein-Jensen medium (c).
Colonies of M. marinum grown on blood (d),
Sabouraud dextrose agar plates (e) and Lowenstein-Jensen medium
(f).
1678-9946-rimtsp-65-S1678-9946202365029-gf03.jpg
PMC10115452_02_1678-9946-rimtsp-65-S1678-9946202365029-gf03.jpg
f03
Initial cultures on blood and Sabouraud agar plates were negative, however, growth was observed on LJ medium (Figure 3F) after 18 days of incubation. Subsequent subcultures were performed on both blood (Figure 3D) and Sabouraud dextrose agar plates (Figure 3E), resulting in the rapid growth of moist, smooth colonies that acquired a yellowish color upon prolonged culture and light exposure.