PMC8217697_01
A: Growth of M. farcinogenes from direct culture on blood agar after 4 days of incubation. B: Subculture on blood agar after 3 days of incubation. C: Acid fast stain from Middlebrook 7H9 Broth. D: Subculture on LJ media after 7 days of incubation.
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After 4 days of incubation, scanty growth of white colonies was observed on routine culture media in three out of four samples (Fig. 1-A), which was identified as Mycobacterium farcinogenes using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (Bruker) with a score of 1.99.
Phylogenetic tree for tested strain; CPHL1437 Mycobacterium spp. (Sequences Alignment and construction of tree were carried out using Phylogeny.fr).
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The phylogenetic relationship of 9 strains which showed more close identity with the resulted sequence (as per NCBI BLAST) is shown in Fig. 2.