PMC10410623_01
Histological findings. A) Hematoxylin and eosin staining shows infiltration of neoplastic cells into the paracortex and sinus area of a lymph node and perinodal fat. B) Neoplastic cells are small to medium in size, with round or cleaved nuclei, inconspicuous nucleoli, and scant eosinophilic cytoplasm.
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These cells were distributed mainly in the lymph node sinus and paracortex with perinodal fat infiltration (Figure 1A, B).
Cytochemical and representative immunohistochemical findings. A) Naphthol AS-D chloroacetate esterase (NASDCAE) is focally positive. B-G) Positive results are obtained for CD33 (B), CD68 (KP1) (C), Iba-1 (D), lysozyme (E), myeloperoxidase (F), and PU.1 (G). H, I) Focal positivity is seen for CD68 (PGM1) (H) and CD163 (I). J, K) CD3 (J) and CD20 (K) are negative. L) Ki-67 labeling index was about 70% in the highest area.
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Cytochemical staining for naphthol AS-D chloroacetate esterase (NASDCAE) yielded focally positive results (Figure 2A). K). Ki-67 labeling index was 40-70% (Figure 2L).
Confocal fluorescence microscopy images. Neoplastic cells coexpress Iba-1 or PU.1 and Ki-67. A) Ki-67 (green), Iba-1 (red), and DAPI (blue). B) Ki-67 (green), PU.1 (red), and DAPI (blue).
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Confocal fluorescence microscopy using Ki-67 and Iba-1 or PU.1 antibodies revealed coexpression of Iba-1 or PU.1 and Ki-67 (Figure 3).