PMC10085002_01
PCR-RFLP assay for analyzing the TLR4-rs4986790 A/G polymorphisms in Sudanese pulmonary Tuberculosis patients.
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Genomic variants of TLR1 (rs5743604, rs5743611, rs5743618, rs76600635, rs5743551, rs5743557, rs5743565, rs5743566, rs5743580, rs5743594 rs4833095, rs5743595, rs5743596); TLR2 (rs1816702, rs5743704 rs5743708, rs7656411, rs11938228, rs893629, rs1898830, rs121917864, rs4696480, rs3804099, rs5743699 rs3804100); TLR4 (rs7869402, rs1927907, rs1927911, rs1927914, rs6478317, rs55912718, rs5030719, rs10759931, rs10759933, rs2770150, rs1554973, rs11536878, rs11536879, rs7873784, rs11536889, rs4986790, rs4986791, rs11536897, rs11536898); TLR6 (rs3796508, rs5743810, rs5743831, rs1039559, rs6531670, rs5743788, rs5743794); TLR8 (rs4830805, rs4830808, rs3747414, rs3761624, rs1548731, rs2109134, rs3788935, rs1013150, rs5744068, rs3764879, rs5744080, rs3764880, rs17256081, rs5741883, rs2407992, rs178998); TLR9 (rs5743836, rs164637, rs352139, rs352140, rs352143, rs352162, rs352165, rs352167, rs187084); and TLR10 (rs4129009, rs7694115, rs10856839, rs11466645, rs4274855, rs11096955, rs11096956, rs11096957, rs7698870, and rs10776483) genes were detected by PCR followed by restriction enzyme fragment analysis (PCR-RFLP) (Figures 1 and 2).
PCR-RFLP assay for analyzing theTLR8-rs3764879 G/C polymorphisms in Sudanese pulmonary  Tuberculosis patients.
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Genomic variants of TLR1 (rs5743604, rs5743611, rs5743618, rs76600635, rs5743551, rs5743557, rs5743565, rs5743566, rs5743580, rs5743594 rs4833095, rs5743595, rs5743596); TLR2 (rs1816702, rs5743704 rs5743708, rs7656411, rs11938228, rs893629, rs1898830, rs121917864, rs4696480, rs3804099, rs5743699 rs3804100); TLR4 (rs7869402, rs1927907, rs1927911, rs1927914, rs6478317, rs55912718, rs5030719, rs10759931, rs10759933, rs2770150, rs1554973, rs11536878, rs11536879, rs7873784, rs11536889, rs4986790, rs4986791, rs11536897, rs11536898); TLR6 (rs3796508, rs5743810, rs5743831, rs1039559, rs6531670, rs5743788, rs5743794); TLR8 (rs4830805, rs4830808, rs3747414, rs3761624, rs1548731, rs2109134, rs3788935, rs1013150, rs5744068, rs3764879, rs5744080, rs3764880, rs17256081, rs5741883, rs2407992, rs178998); TLR9 (rs5743836, rs164637, rs352139, rs352140, rs352143, rs352162, rs352165, rs352167, rs187084); and TLR10 (rs4129009, rs7694115, rs10856839, rs11466645, rs4274855, rs11096955, rs11096956, rs11096957, rs7698870, and rs10776483) genes were detected by PCR followed by restriction enzyme fragment analysis (PCR-RFLP) (Figures 1 and 2).