PMC4772463_01
Maximum Likelihood tree illustrating the phylogenetic position of K. capensis and K. proteae in the Gondwanamycetaceae (grey block). The Protea-associated species are shaded red and the two isolates for which genome sequences were determined are indicated with a box. The sequences of the Internal Transcribed Spacer (ITS) region (available from GenBank , accession numbers in brackets following isolate numbers) were aligned in MAFFT 7. The phylogeny was calculated in MEGA6 using the Tamura-Nei substitution model, 1000 bootstrap replicates and Ceratocystis fimbriata (Ceratocystidaceae) as an outgroup
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PMC4772463_01_40793_2016_139_Fig1_HTML.jpg
Fig1
The new isolates (Figs. 1 & 2) were collected from the same localities and hosts as the holotype specimens: K. capensis (CMW40890 = CBS139037) from the infructescences of P. longifolia Andrews in Hermanus, and K. proteae (CMW40880 = CBS140089) from P. repens infructescences in Stellenbosch, both locations in the Western Cape Province of South Africa.
Sexual sporing structures of the two Knoxdaviesia species sequenced in this study. K. capensis (a) and K. proteae (b) were sampled from Protea longifolia and P. repens flowers, respectively. Scale bars = 1 mm
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PMC4772463_01_40793_2016_139_Fig2_HTML.jpg
Fig2
The new isolates (Figs. 1 & 2) were collected from the same localities and hosts as the holotype specimens: K. capensis (CMW40890 = CBS139037) from the infructescences of P. longifolia Andrews in Hermanus, and K. proteae (CMW40880 = CBS140089) from P. repens infructescences in Stellenbosch, both locations in the Western Cape Province of South Africa.