PMC4963584_01
Representative pictures of histopathological analysis performed on formalin-fixed and paraffin embedded kidney tissue section obtained from a first (a-b) and a second kidney punction-biopsy (c-d) performed in patient with membranous nephropathy. (a) Haematoxylin eosin, Jone's silver (b), and Masson Trichrome (c-d) standard stainings. (a) Absence of tubulointerstitial involvement and (b) marked thickening of glomerular basement membrane with stubby spikes-like projections corresponding to stage 2 of membranous nephropathy. (c) Tubular atrophy (tubular dilatation and flattening of epithelium), sparse interstitial inflammation, and fibrosis. (d) Marked thickening of glomerular basement membrane with double contours. Original magnifications: (a) x40, (b) x1000 (kindly provided by Dr. Selda Aydin, Pathology Department, St. Luc Hospital, UCL, Brussels, Belgium), (c) x40, and (d) x400 (kindly provided by Dr. Michel Depierreux, Pathology Department, Erasme Hospital, CUB, ULB, Brussels, Belgium).
MI2016-7651024.001.jpg
PMC4963584_01_MI2016-7651024.001.jpg
fig1
Optical and electron microscopy analyses of kidney tissue biopsy were performed in another hospital at that time and showed glomerular lesions typical for membranous nephropathy (Figures 1(a) and 1(b)).