PMC4533452_01
Intraoperative photographs and fluorescence angiograms of the illustrative case. Regular microscopic view (A), intra-arterial angiography via the superficial temporal artery at arterial phase (B), capillary phase (C), and venous phase (D) and intra-venous angiography at arterial phase (E), capillary phase (F), and venous phase (G) are shown. Bright fluorescence emission with quick clearance was observed in intra-arterial angiography.
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F4
Fluorescence was washed out from the arteries and capillaries without delay, and during the venous phase fluorescence had already disappeared from the arteries and capillaries (Fig. 4).
Changes in fluorescence intensity at various ROIs by intra-arterial injection via the superficial temporal artery (A) and intra-venous injection (B) and regular microscopic view (C) in the illustrative case. ROIs were set at the M1 segment of the middle cerebral artery (red open square), the M2 segment (blue open square), the frontal lobe (green open square), and the vein (orange open square), and changes in fluorescence intensity at each ROI were plotted. Blood flow could be independently evaluated during the arterial, capillary, and venous phase because of the quick clearance of fluorescence by intra-arterial injection (A). It took only a minute and more than 5 minutes to decrease fluorescence intensity at the base line before administration in intra-arterial angiography and intra-venous fluorescence angiography, respectively (A, B). ROI: region of interest.
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F5
When the ROIs were set at M1 and M2, the fluorescence intensity graph showed M2 had almost the same peak as M1 (Fig. 5).