PMC5471746_01
Presence of chorioretinal lesions in the tapetal fundus OD noted on initial presentation. Images represent central view (A) and a view angled to highlight the largest chorioretinal lesion (B). Multifocal pink lesions are noted throughout the tapetal fundus arising from the termination of retinal vessels representing metastatic spread of the hemangiopericytoma. Images obtained with the RetCam Shuttle.
OpenVetJ-7-132-g001.jpg
PMC5471746_01_OpenVetJ-7-132-g001.jpg
F1
Upon indirect ophthalmoscopic examination (Vantage Plus Wireless, Keeler Instrument Inc, Bromall, PA) of the OD, multifocal lesions in the tapetal fundus were noted and photodocumented (RetCam Shuttle; Clarity Medical Systems, Pleasanton, CA) (Fig. 1A and 1B).
(A): Metastatic spindle cell sarcoma. Subgross photograph. Note the carpet of neoplastic cells in the choroid (arrow) and in the ciliary body (*). Hematoxylin and eosin (H&E). (B): Tumor in the choroid. Higher magnification of the neoplastic cells near the arrow in Fig. 2A. Cells infiltrate the tapetum (*) and surround choroidal vessels (arrow). (H&E) (Bar = 100 mum). (C): Tumor in the scleral vessels. Higher magnification of the square in Fig. 2A. Cells surround and infiltrate scleral vessels (arrow). (H&E). (Bar = 100 mum).
OpenVetJ-7-132-g002.jpg
PMC5471746_01_OpenVetJ-7-132-g002.jpg
F2
Histopathology revealed a population of neoplastic spindle cells arranged in streams and bundles carpeting the surfaces of the iris and ciliary body, infiltrating and expanding the tapetal choroid and multifocally invading the choroidal vessels (Fig. 2A and 2B). Neoplastic cells were also found to extend within scleral blood vessels (Fig. 2C).
Immunohistochemical staining of both the original right flank/limb hemangiopericytoma and the left eye metastasis. Both tissues show positive staining for vimentin and alpha-smooth muscle actin (SMA).
OpenVetJ-7-132-g003.jpg
PMC5471746_01_OpenVetJ-7-132-g003.jpg
F3
The results of the immunohistochemical staining showed perfect alignment in staining patterns in that the two separate masses were vimentin, alpha-smooth muscle actin and CD34 positive, while negative for skeletal muscle actin, S-100 and CD31 (Fig. 3).
Image of right tapetal fundus two weeks after initial presentation. The presence of numerous large pink/red colored lesions throughout tapetal fundus present near retinal vessel terminations showing significant progression of chorioretinal lesions. Perilesional retinal elevations are seen around each foci of metastasis and appear as halos of reduced reflectivity. Image obtained with the RetCam Shuttle.
OpenVetJ-7-132-g004.jpg
PMC5471746_01_OpenVetJ-7-132-g004.jpg
F4
No changes in the lenticular opacities were observed, however, fundic examination revealed that the chorioretinal lesions had progressed significantly in size and number throughout the tapetal fundus (Fig. 4).