PMC7185820_01
Photomicrograph of left ventricular apical specimen showing lymphocytic infiltrate within the myocardium, associated with myocardial fibre damage and necrosis. H and E staining at magnification of 200x.
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Histological examination of the left ventricular apical tissue revealed features in keeping with lymphocytic viral myocarditis (Fig. 1 ), as well as microscopic foci consistent with myocardial ischaemia.
Serial serum PCRs for HPIV-1-3 and their response to ribavirin therapy. Oral ribavirin was given on days 1 and 2 while IV ribavirin was started from day 3.
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UltraTM polymerase. Utilizing these primer sets, the patient's serum also tested positive for HPIV-2 by PCR indicating an ongoing HPIV-2 viraemia (Fig. 2). Following IV ribavirin initiation, viraemia clearance was monitored using the PEID RT-PCR assay designed to detect 1062 bp of the HPIV-2 genome and showed a serial decline in HPIV viraemia with clearance on day 4 of ribavirin therapy (Fig. 2 ) along with improvement in myocardial inflammation as reflected by the normalization of the cardiac enzymes (Fig. 3 ).