PMC3505916_01
(a) G-band karyotype analysis performed at diagnosis revealed the following karyotype: 46,XY,t(9;22;16)(q34;q11;q24). (b) Dual-color FISH analysis performed using a BCR/ABL-specific probe (LSI BCR/ABL Dual-Color single translocation probe, Vysis, IL, US) demonstrated the presence of fusion signals (white arrow) in CML cells.
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fig1
Bone marrow aspiration revealed less than 5% blasts, and karyotype analysis of his bone marrow cells showed the following karyotype: 46,XY,t(9;22;16)(q34;q11;q24) [20] (Figure 1(a)). In addition, fluorescent in situ hybridization showed BCR/ABL fusion signals in 92.2% cells, as shown in Figure 1(b).
Multicolor FISH image (pseudocolor labeled) of metaphase spreads after spectrum-based classification.
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fig2
As shown in Figure 2, the complex translocation found by multicolor-FISH was consistent with the results of the G-banding analysis.