PMC5286497_01
(a) Both components of the histologic picture are seen in this section. Small-to-medium-sized lymphoid cells with clear cytoplasm and eosinophils are segregated by intervening whorls of spindly follicular dendritic cells (H&E x320). (b) Numerous PD1+ cells are found in the cellular area of the lesion consistent with AITL (Immunohistochemistry with DAB x320). (c) Several whorls of follicular dendritic cells are prominent in this section (H&E x320). (d) The spindle follicular dendritic cells show very strong CD21 immunostaining. Note the negative staining related to the high endothelial venules (Immunohistochemistry with DAB x320).
CRIHEM2017-9601094.001.jpg
PMC5286497_01_CRIHEM2017-9601094.001.jpg
fig1
On low power microscopy, the lymph node structure was effaced and showed two distinct types of histological features (Figure 1(a)). The first consisted of highly cellular areas, composed of small-to-medium-sized cells, often with a clear cytoplasm; these were predominantly T-cells: CD3+++; CD2+++; CD4+++; CD8++; CD5+++; CD7+/(-); PD1++ (Figure 1(b)), in a polymorphous cellular background, including eosinophils and isolated large CD20+ cells. The other aspect of the histology was composed of spindle cells with small uniform nuclei, arranged in whorls formations (Figure 1(c)). These cells were CD21+++ (Figure 1(d)); CD23+++; fascin ++; HLA-DR++.