PMC4697348_01
Pulsed field gel electrophoresis (PFGE) was performed by using the CHEF-DRII System (Bio-Rad, Hercules, CA, USA) at 6 V/cm for 20 hr at 10C, with initial and final pulse times of 0.5 sec and 30 sec, respectively. XbaI was the restriction enzyme used. All three clinical isolates from our hospital (hospital C) exhibited 100% identical PFGE banding patterns. This finding suggests that sequentially identified B. petrii were the same strains. Lane C1-3, Bordetella petrii isolated from patient's wound; ATCC, ATCC DSM 12804.
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F1
All three clinical isolates exhibited identical PFGE banding patterns (Fig. 1).