PMC9273825_01
Computerized Tomography scan of the chest revealed a large tumor on the right posterior upper chest wall. (A) coronal plane. (B) sagittal plane.
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The admission CT showed that there was a prominent mass on the right posterior upper chest wall invading the adjacent vertebral body but the boundary between the lesion and the surrounding tissues was relatively clear (Figures 1A,B).
Gross photograph of the sectioned specimens from revealed a gray-yellow gray-brown foliated nodular tumor.
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The cut surface was grayish-yellow-white, solid, and tough, with mucinous degeneration in some specific areas (Figure 2).
H&E staining results. (A-C) The tumor-rich areas, collagen fiber sclerosis areas and mucus areas were found to be staggered (40X). (D,E) The tumor cells were embedded in the fibrous matrix, and small and medium-sized epithelioid cells were arranged in cords and nests (100X). (F) The tumor invaded the bone of the spine (40X).
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Fatty changes could be seen locally in some specific areas (Figure 3).
IHC and FISH analysis results. (A) Positive control for MUC4 showing normal (colon glands), 100X. (B) The tumor cells were diffusely positive for MUC4 in the cytoplasm,100X. (C) FISH analysis using the EWSR1 dual-color break-apart probe showed one red/green fused signal and one or two red split signals per nucleus in several tumor cells (shown by arrows),1000X. (D) FISH analysis using FUS dual-color break-apart probe revealed more than two red/green fused signals per nucleus in most tumor cells (shown by arrow), 1000X.
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However, none of these were tested to determine their genetic partners or fusion breakpoints (Figures 4C,D).