PMC3420446_01
Microphotographs of the lesion in the occipital lobe showing a sharply demarcated area of demyelination ((a) and (b) Luxol-Fast-Blue, magnification 4X), reactive astrogliosis ((c), (h), and (e) stain, magnification 100X), and unaffected nerve fibers ((d) Bodian stain, magnification 200X). Note the perivascular lymphocytic infiltrates ((e) Luxol-Fast-Blue stain, magnification 100X), perivascular T-lymphocytic infiltrates and lymphocytes in the surrounding parenchyma ((f) UHCL antibody, magnification 200X), and activated macrophages/microglia within the demyelinated area (CR3/43 antibody; (g) magnification 25X, (h) magnification 400X).
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fig1
In the white matter of the occipital lobe, two sharply demarcated areas of demyelination were evident (Figures 1(a) and 1(b)) associated with reactive astrogliosis (Figure 1(c)). The axons were well preserved on Bodian silver impregnation (Figure 1(d)). T-lymphocytes as seen on immunostaining with UCHL (CD45RO) antibody (Figure 1(e)) as well as numerous CD68- and CR3/43-immunopositive macrophages/microglial cells (Figures 1(g) and 1(h)). The cerebellum and brain stem were unremarkable.
Gas chromatography-mass spectrometry (GCMS) of the brain tissue: single-ion monitoring for amphetamine (target ion 86 dalton, retention time 5.33 mins). Calibration was performed with spiked serum samples up to 0.1 mg/L and led to a concentration of 0.017 mug/g brain tissue.
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PMC3420446_01_CRIM.PATHOLOGY2011-514613.002.jpg
fig2
Analysis of brain samples of the occipital region including the demyelinated area revealed an amphetamine content of 0.017 mug/g determined by GC/MS (Figure 2).