PMC3964430_01
Part of CSGE Gel Showing the Migration Pattern of Exon 14F Amplified Fragments. Fragments With Abnormal Migration Patterns are Marked by Arrow. N: Normal Control, L: Ladder.
ircmj-16-6727-g001.jpg
PMC3964430_01_ircmj-16-6727-g001.jpg
fig8433
Among all amplified fragments, only PCR product of exon 14F with 686bp long, displayed unmatched shift in CSGE (Figure 1).
DNA Sequencing Profile of the FVIII Gene (Exon 14F) from the Patient and Normal Controls. This Figure Indicates Transition Point Mutation (GAA GGA) in the Patient DNA Sequence that Replaces a Glutamic acid With a Glycine Residue. Underlining Indicates the Position of Point Mutation.
ircmj-16-6727-i001.jpg
PMC3964430_01_ircmj-16-6727-i001.jpg
fig8434
PCR product (Figure 2). PCR reaction mixture for exon 14 F containing 1 x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP, 10 pmoles of each primer (Forward: 5'-TCCCTACGGAAACTAGCAATG-3' and Reverse: 5'-TCACAAGAGCAGAGCAAAGG-3'), 0.5U of