PMC5996049_01
Histopathology of a Sweet's syndrome lesion. Closer views (A) of Sweet's syndrome lesions located on the upper arms are shown. The biopsy specimen (B) shows a confluent neutrophilic infiltrate in the reticular dermis and edema in the papillary dermis (hematoxylin and eosin staining).
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F1
Pathohistological work up of skin lesions revealed papillary dermal edema, swollen endothelial cells, and a diffuse infiltrate of predominantly neutrophils with leukocytoclasia (Figure 1).
Bone marrow aspiration at diagnosis (A,B). Hypocellular bone marrow with 14% blasts and dysplasia in all cell lineages (May-Giemsa).
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F2
Due to persistent cytopenia, bone marrow aspiration was also performed, showing 14.2% blasts with dysplasia in all cell lineages (Figure 2).
Frequency of regulatory T cells within CD4-positive T cell compartment in peripheral blood (PB). Flow cytometry of PB gated on CD3-positive in upper panel and CD3 CD4-double positive T cells in lower panel. Numbers indicate percentage in each quadrant. I CD4+CD45RA+forkhead box p3 (FoxP3) low naive Treg, II CD45RA-FoxP3 high effector Treg, III CD45RA-FoxP3 low non-Treg.
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F3
T cells (Treg cells) staining we used the anti-human forkhead box p3 (FOXP3) BV421 conjugate after fixation and permeabilization according to manufacturer's instructions (Becton-Dickinson). Therapy with 5-Aza led to no significant increase in the absolute lymphocyte count with changes in the distribution of the CD4-positive T cells within the entire compartment (Figure 3). Especially, 5-Aza treatment led to an increase in the frequency of naive and effector Treg cells (Fraction I and II, respectively, Figure 3).