PMC2633208_01
Gram-stained smear of peritoneal dialysate showing scattered gram-negative coccobacilli (arrows) among neutrophils.
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F1
The peritoneal dialysate was turbid, and contained 5,100/microL leucocytes with 99% of neutrophils and gram-negative coccobacilli (Fig. 1).
Sheep blood agar plate showing 1-2 mm sized, raised, grayish-white colonies after 48 hr incubation at 37CC, 5%-CO2.
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F2
Culture of the peritoneal dialysate on sheep blood agar grew 0.5-1.0 mm sized flat, grey-white, non-hemolytic colonies after 24 hr of incubation at 35C and 5-10% CO2 (Fig. 2).
Gram-stained smear of colony on BAP after 48 hr incubation at 37CC showing gram negative coccobacili.
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PMC2633208_01_jkms-24-S215-g003.jpg
F3
The organism was a gram negative coccobacillus (Fig. 3), and also grew readily on MacConkey and salmonella-shigella agar.