PMC4602630_01
(A) Frozen transverse section of the sural nerve of patient 1 stained with anti-CD20 antibody. Several vessels in the epineurium are surrounded by B-cell infiltrates. (B) Electron micrograph of tranverse section of the sural nerve of patient 1 showing the typical widening of the most external myelin lamellae. (C) Immunoelectron micrograph of patient 2. Anti-kappa light chain immunogold staining shows that the monoclonal protein of the patient has infiltrated the myelin sheath. (D) Frozen transverse section of the sural nerve of patient 3 stained with anti-CD20 antibody. There is a massive infiltrate of B-cells in the epineurium.
medi-94-e394-g003.jpg
PMC4602630_03_medi-94-e394-g003.jpg
F1
CD45+ T-cells) and some CD68+ macrophages (also in the endoneurial space) were observed (Figure 1D). Clonal B-cells were detected by conventional techniques (see above). Congo red-stained sections were normal.
PMC4602630_02
(A) Frozen transverse section of the sural nerve of patient 1 stained with anti-CD20 antibody. Several vessels in the epineurium are surrounded by B-cell infiltrates. (B) Electron micrograph of tranverse section of the sural nerve of patient 1 showing the typical widening of the most external myelin lamellae. (C) Immunoelectron micrograph of patient 2. Anti-kappa light chain immunogold staining shows that the monoclonal protein of the patient has infiltrated the myelin sheath. (D) Frozen transverse section of the sural nerve of patient 3 stained with anti-CD20 antibody. There is a massive infiltrate of B-cells in the epineurium.
medi-94-e394-g003.jpg
PMC4602630_03_medi-94-e394-g003.jpg
F1
CD45+ T-cells) and some CD68+ macrophages (also in the endoneurial space) were observed (Figure 1D). Clonal B-cells were detected by conventional techniques (see above). Congo red-stained sections were normal.
PMC4602630_03
(A) Frozen transverse section of the sural nerve of patient 1 stained with anti-CD20 antibody. Several vessels in the epineurium are surrounded by B-cell infiltrates. (B) Electron micrograph of tranverse section of the sural nerve of patient 1 showing the typical widening of the most external myelin lamellae. (C) Immunoelectron micrograph of patient 2. Anti-kappa light chain immunogold staining shows that the monoclonal protein of the patient has infiltrated the myelin sheath. (D) Frozen transverse section of the sural nerve of patient 3 stained with anti-CD20 antibody. There is a massive infiltrate of B-cells in the epineurium.
medi-94-e394-g003.jpg
PMC4602630_03_medi-94-e394-g003.jpg
F1
CD45+ T-cells) and some CD68+ macrophages (also in the endoneurial space) were observed (Figure 1D). Clonal B-cells were detected by conventional techniques (see above). Congo red-stained sections were normal.
PMC4602630_04
(A) Paraffin-embedded transverse section of the sural nerve of patient 4 stained with hematxylin-eosin, showing perivascular infiltrate of mononuclear cells in the epineurium. (B) Paraffin-embedded longitudinal section of the radial nerve of patient 5, showing massive infiltrates of mononuclear cells in the epineurium. (C) Frozen tranverse section of the sural nerve of patient 7 stained with anti-CD45 antibody, showing a perivascular epineurial T-cell infiltrate. (D) Paraffin-embedded transverse section of the sural nerve of patient 8, stained with hematoxylin-eosin. Two middle-size vessels of the epineurium display the typical aspect of necrotizing vasculitis.
medi-94-e394-g004.jpg
PMC4602630_08_medi-94-e394-g004.jpg
F2
Lesions of necrotizing vasculitis (with infiltration by numerous mononuclear cells and fibrinoid necrosis of the vessel walls of middle-sized epineurial arterioles) were observed (Figure 2D).
PMC4602630_05
(A) Paraffin-embedded transverse section of the sural nerve of patient 4 stained with hematxylin-eosin, showing perivascular infiltrate of mononuclear cells in the epineurium. (B) Paraffin-embedded longitudinal section of the radial nerve of patient 5, showing massive infiltrates of mononuclear cells in the epineurium. (C) Frozen tranverse section of the sural nerve of patient 7 stained with anti-CD45 antibody, showing a perivascular epineurial T-cell infiltrate. (D) Paraffin-embedded transverse section of the sural nerve of patient 8, stained with hematoxylin-eosin. Two middle-size vessels of the epineurium display the typical aspect of necrotizing vasculitis.
medi-94-e394-g004.jpg
PMC4602630_08_medi-94-e394-g004.jpg
F2
Lesions of necrotizing vasculitis (with infiltration by numerous mononuclear cells and fibrinoid necrosis of the vessel walls of middle-sized epineurial arterioles) were observed (Figure 2D).
PMC4602630_06
PMC4602630_07
(A) Paraffin-embedded transverse section of the sural nerve of patient 4 stained with hematxylin-eosin, showing perivascular infiltrate of mononuclear cells in the epineurium. (B) Paraffin-embedded longitudinal section of the radial nerve of patient 5, showing massive infiltrates of mononuclear cells in the epineurium. (C) Frozen tranverse section of the sural nerve of patient 7 stained with anti-CD45 antibody, showing a perivascular epineurial T-cell infiltrate. (D) Paraffin-embedded transverse section of the sural nerve of patient 8, stained with hematoxylin-eosin. Two middle-size vessels of the epineurium display the typical aspect of necrotizing vasculitis.
medi-94-e394-g004.jpg
PMC4602630_08_medi-94-e394-g004.jpg
F2
Lesions of necrotizing vasculitis (with infiltration by numerous mononuclear cells and fibrinoid necrosis of the vessel walls of middle-sized epineurial arterioles) were observed (Figure 2D).
PMC4602630_08
(A) Paraffin-embedded transverse section of the sural nerve of patient 4 stained with hematxylin-eosin, showing perivascular infiltrate of mononuclear cells in the epineurium. (B) Paraffin-embedded longitudinal section of the radial nerve of patient 5, showing massive infiltrates of mononuclear cells in the epineurium. (C) Frozen tranverse section of the sural nerve of patient 7 stained with anti-CD45 antibody, showing a perivascular epineurial T-cell infiltrate. (D) Paraffin-embedded transverse section of the sural nerve of patient 8, stained with hematoxylin-eosin. Two middle-size vessels of the epineurium display the typical aspect of necrotizing vasculitis.
medi-94-e394-g004.jpg
PMC4602630_08_medi-94-e394-g004.jpg
F2
Lesions of necrotizing vasculitis (with infiltration by numerous mononuclear cells and fibrinoid necrosis of the vessel walls of middle-sized epineurial arterioles) were observed (Figure 2D).