PMC6882502_01
Magnetic resonance imaging results. T1-weighted images (A) showed multiple oval hypointense lesions in the white matter arround bilateral. These lesions were hyperintense on T2-weighted images (B) and fluid-attenuated inversion recovery images (C). Diffusion-weighted images (DWI) demonstrated several increased signal intensity lesions in the white matter arround bilateral ventricles (D).
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F1
MRI of the brain showed multiple lesions more marked in the white matter around bilateral ventriculus cerebri lateralis (Figures 1A-D).
Pathogen identification from cerebrospinal fluid sample using next-generation sequencing method. (A) The number of sequencing reads identified corresponding to Human polyomavirus 2 [also known as JC virus ] was 34; with genome coverage 35.1825%. (B) Reads distribution of total DNA sequence in the CSF sample without human host.
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F2
Thus, we noted 34 sequencing reads uniquely aligned to Human polyomavirus 2 genome (Figure 2B), and these reads enclosed a high coverage percentage (35.18%) of Human polyomavirus 2 genome (Figure 2A).
Maximum likelihood phylogenetic tree of Human polyomavirus. Human polyomavirus 2 AB103411 (in red) was the most similar strain in the cerebrospinal fluid of the patient.
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F3
The phylogenetic tree presenting our sequence similarities with other Human polyomavirus is shown in Figure 3.
Human polyomavirus 2 detection by PCR. Lane 1: 129-bp PCR product of the primer: F-GGTTTAGGCCAGTTGCTGACTT, R-GTCTCCCCATACCAACATTAGCTT; Lane M: DNA ladder. Lane 2: Negative control.
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F4
The PCR products (Figure 4) were sequenced and then mapped to the nucleotide database with the online NCBI blast (https://blast.ncbi.nlm.nih.gov/Blast.cgi).