PMC3715369_01
Radiologic findings on serial HRCTs performed at the time of admission (A, B) and 10 months later following 4 months of successful smoking cessation (C, D). Abnormalities suggestive of PLCH are a combination of multiple cysts and nodules predominantly in the middle and upper lung zones.
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High-resolution computed tomography (HRCT) scan of the chest showed abnormalities typical for PLCH (Figure 1A and 1B). This time the HRCT showed significant improvement (Figure 1C and 1D).
2-DE images of the BALF proteome in a healthy subject (A), a case of COPD (B), and a case of PLCH (C). 50 mug proteins were separated by isoelectric focusing (pI 3-10) in the first dimension and by SDS-PAGE (Mr 10-250 kDa) in the second dimension. The proteins were visualized with silver staining and the typical BALF proteins identified by pattern matching. 1 surfactant protein A, 2 apolipoprotein A1, 3 haptoglobin, 4 immunoglobulin G light chain, 5 haptoglobin fragment, 6 alpha2-HS-glycoprotein, 7 alpha1-antitrypsin, 8 albumin, 9 hemopexin, 10 gelsolin, 11 transferrin, 12 lactoferrin, 13 immunoglobulin G heavy chain, 14 CC10, 15 transthyretin, 16 cystatin S, 17 pro-apolipoprotein, 18 amyloid P, 19 calgranulin B, 20 calgranulin A, 21 orosomucoid, 22 immunoglobulin A.
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Figure 2 shows 2-DE images of the different BALF proteomes. About 340 protein spots were detected in the BALF from the healthy lung (Figure 2A); most of the proteins had a molecular weight of 30-70 kDa and an isoelectric point (pI) between 4.5 and 7.0. BALF from the COPD human lung (Figure 2B), about 250 spots were detected and the overall 2-DE image was rather similar to that from the healthy subject. By contrast, in the BALF from the PLCH case (Figure 2C) only 140 spots were detected and the overall 2-DE image was clearly different.