PMC5370587_01
PMC5370587_02
PMC5370587_03
RJ Histopathologic features: Formalin-fixed, paraffin-embedded 5-micron sections of the globus pallidus were prepared by standard methods and stained with hematoxalin and eosin (H&E, Panel A) or with H&E with luxol fast blue (LFB) myelin stain (Panel B). Myelin pallor, neuronal loss and gliosis were widespread throughout affected brain regions. Panel C demonstrates an H&E/LFB-stained section of control globus pallidus for comparison. Scale bars in Panels A-C are all 100 mum. Panel D depicts a lower-magnification view of the medulla, including the inferior olivary nucleus (ION) and the dorsal accessory olivary nucleus (DAO); dorsal to this, in the left half of the image, lie the ventral respiratory group and nucleus ambiguus, which also demonstrate architectural effacement due to degenerative changes similar to those seen in the globus pallidus. Scale for Panel D is 500 mum.
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F1
Figure 1 shows the loss of neurons and demyelination associated with axonal degeneration in the globus pallidus and medulla.
RJ Taupathy: Immunohistochemical stains for tau were performed on paraffin sections with the use of PHF-1 antibody after antigen retrieval and show characteristic tau-positive astrocytes and dystrophic neurites in the globus pallidus. Scale bar is 100 mum.
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F2
Cytoplasmic tau-positive lesions were seen in astrocytes and neurons in affected brain regions, in a background of many tau-positive neurites (Figure 2).
PHF-1 immunohistochemistry of the VRG of representative PSP case including RJ (3E). All cases but A show striking tauopathy. Scale bar is 40 mum for all panels.
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F3
RJ showed extensive tauopathy in this area, more striking than in the globus pallidus (Figure 3).
PMC5370587_04