PMC8749139_01
T. asahii morphology on culture and microscopy, subcultures from the positive blood culture bottle: (a) On candida medium biplate after 4 days of incubation. Left side with dry blue/petrol-coloured colonies on chromogenic sabouraud agar, right side with white to cream-coloured colonies with raised surfaces on sabouraud agar. (b) Dermasel agar plate after 4 days of incubation showing brilliant-white fluffy growth. (c) Microscopic morphology of T. asahii: septate hyphae and arthroconidia (lactophenol cotton blue wet mount). (d) Gram-staining. (e) differential interference contrast microscopy.
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F1
Gram-staining (Fig. 1d) revealed yeast-like structures and sub-culture on Sabouraud agar was performed (Fig. 1a, b). A lactophenol cotton blue wet mount (LPCB) was performed with the material from the agar plates showing septate hyphae and arthroconidia (Fig. 1c).
PMC8749139_02
Binding regions of primers and the probe of the fungal PCR array. ITS: nuclear ribosomal internal transcribed spacer region. The binding region of the probe is marked in orange.
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F2
With reaction F (Fig. 2), Trichosporon spp. Two further branch-specific reactions (B and C, Fig. 2) showed cross-positivity.
ITS-Sequence alignments.
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F3
Sequencing of the PCR product with subsequent homology search via blast and CBS-database allowed species identification of T. asahii in all samples (Fig. 3).