PMC8511908_01
Amplification plot showed RT-PCR detection of SARS-CoV-2 E gene (green curve, Ct 37.73)
and RdRp gene (green curve, CT 38.17), together with the human RNA internal standards
(purple lines) (Bio-Rad CFX96 instrument, Bio-Rad CFX Maestro 1.1 software).
10.1177_20499361211048572-fig1.jpg
PMC8511908_01_10.1177_20499361211048572-fig1.jpg
fig1-20499361211048572
The CSF was tested in three replicates for each gene and was considered clearly positive at cycle threshold (Ct) of 38.94, 37.22, and 38.17, respectively, for the RdRp gene (Figure 1). The time between the first positive nasopharyngeal SARS-CoV-2 RT-PCR and the positive CSF was 114 days (from the positivity in October) and was 62 days from the separate onset of symptoms on 23 November (since we do not have genomic analysis to distinguish a relapse from a reinfection) - (ZymoResearch: CA, USA), (Bio-Rad Laboratories: CA, USA).