PMC5574304_01
Basophil activation test with maltitol. The G2 quadrant of the dot plots represents the percentage of basophils that expresses CD63 in high intensity (activation of cells). (a) Negative control; (b) positive control; (c) maltitol at 1000 microg/ml; (d) maltitol at 100 microg/m.
CRIM2017-2127167.002.jpg
PMC5574304_01_CRIM2017-2127167.002.jpg
fig2
Double staining was carried out with CD203c-PE to detect basophils and CD63-FITC to detect basophil activation (Figure 2). Serum saline was added as negative control (Figure 2(a)), showing the basally activated basophils, before adding any stimulus. The peptide fMLP was used as positive control (Figure 2(b)), to asses an adequate cellular reactivity.