PMC2782172_01
A nodular submucosal tumor is noted on the left side of hard palate.
jkms-20-687-g001.jpg
PMC2782172_01_jkms-20-687-g001.jpg
F1
A 34-yr-old male patient was admitted our hospital with a painless swelling of the left hard palate (Fig. 1).
The paranasal CT scan showing a contrast-enhancing mass lesion in left side hard palate with focal bony destruction (*).
jkms-20-687-g002.jpg
PMC2782172_01_jkms-20-687-g002.jpg
F2
A paranasal sinus CT-scan revealed a contrast-enhancing mass lesion in left side hard palate with focal bony destruction (Fig. 2).
Light microscopy of the tumor shows closely packed small round cells with abundant thin-walled vessels (H&E, x40).
jkms-20-687-g003.jpg
PMC2782172_01_jkms-20-687-g003.jpg
F3
Histological examination of hematoxylin-eosin stained sections showed a homogeneous population of closely packed small neoplastic cells with abundant fibrovascular stroma (Fig. 3).
Acini of minor salivary gland are seen at the periphery of tumor (arrow) (H&E, x10).
jkms-20-687-g004.jpg
PMC2782172_01_jkms-20-687-g004.jpg
F4
Remnant salivary gland tissue was observed at the tumor's periphery (Fig. 4).
Most of the tumor cells have scanty cytoplasm and round or oval nuclei with fine powdery chromatin (H&E, x400).
jkms-20-687-g005.jpg
PMC2782172_01_jkms-20-687-g005.jpg
F5
Most of the individual cells had scanty cytoplasm, indistinct borders and round or oval nuclei with fine powdery chromatin (Fig. 5).
Strong membranous MIC2/CD99 immunoreactivity (LSAB, x200).
jkms-20-687-g006.jpg
PMC2782172_01_jkms-20-687-g006.jpg
F6
The CD99/MIC2 stain exhibited strong membranous staining (Fig. 6).
EWS/FLI1 chimeric gene of approximately 260 bp is identified by RT-PCR in this case (arrow) (M, size marker; lane 1, positive control; lane 2, negative control; lane 3, this case).
jkms-20-687-g007.jpg
PMC2782172_01_jkms-20-687-g007.jpg
F7
The RT-PCR showed the presence of the EWS-FLI-1 fusion transcript, which is of the t(11;22) translocation characteristic of ES and primitive neuroectodermal tumor (Fig. 7).