PMC8143047_01
Positron emission tomography-computed tomography (PET-CT). (A, B) Disseminated lymphadenopathy due to non-tuberculous mycobacterial (NTM) infection (red arrows) and bone-marrow activation and hepatosplenomegaly due to hemophagocytic lymphohistiocytosis (HLH) at diagnosis. (C, D) Control PET-CT following allogeneic hematopoietic transplantation (day +240) and after 12 months of tuberculostatic therapy demonstrating complete remission of NTM-related lymphadenopathy and remission of HLH-associated hematopoietic activation.
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These findings were confirmed in positron emission computer tomography (PET-CT) scanning ( Figures 1A, B ). PET-CT scanning at that time demonstrated complete remission of all inflammatory manifestations ( Figures 1C, D ).
CD107a degranulation assay. Degranulation of CD56+ NK-cells (A) and CD8+ CTL (B) was examined as previously published7. In brief, CD107a-expression on cell surfaces was analyzed by flow-cytometry in resting cells (A, B;
left dot plot diagrams) and subsequent to 48 h stimulation with interleukin 2 (IL-2) of NK-cells (A, right dot plot diagrams), and phytohemagglutinin (PHA)/IL-2 of CD8+ T-cells (B, right dot plot diagrams), respectively.
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These indicated significant degranulation impairments in both effector cell types ( Figure 2 ).