PMC8677407_01
Flow cytograms of a liver aspirate sample. (a) Two scatter gates are drawn around granulocytes (larger, more complex cells) and lymphocytes (smaller, less complex cells). (b) Approximately 71% of the cells in the lymphocyte gate are positive for CD5 (T-cell marker) and negative for CD21 (B-cell marker). (c) The vast majority of these cells (~96%) are negative for the T-cell subset antigens CD4 and CD8.
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fig1
T-cell marker) but no lineage-specific subset markers (CD4- and CD8-). This finding was considered diagnostic for lymphoma based on aberrant T-cell marker expression, in conjunction with the cytology findings (Figure 1).
Necropsy images illustrating diffuse icterus, mottled liver, and severely dilated biliary system. Asterisk (*) denotes the gallbladder in all images. (a) The arrow shows the full thickness duodenal ulceration. (b) The duodenum was opened, and a red rubber was passed through the major duodenal papilla (arrow) into the severely dilated common bile duct. (c) The arrow denotes the severely dilated hepatic ducts joining the cystic duct into the common bile duct. (d) The common bile duct was incised showing purulent discharge and a large stone lodged at the major duodenal papilla (arrow).
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fig2
The gross pathology examination showed marked dilation of the gallbladder and common bile duct (Figure 2).