PMC7732509_01
Timeline of the procedures and observations realized on the treated stallion and the Przewalski's horseband.
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F1
On day 0 (May 26th 2016), day 32, and day 177, the vaccine was subcutaneously administrated in the chest, during the procedures performed under general anesthesia to assess the effects of immuno-neutering on semen quality and testicular endocrine activity with semen and blood samples (Figure 1). The treated stallion's paternity was genetically excluded in foals born during these 2 years (Figure 1).