PMC4448044_01
a Multifocal loss of subcutaneous fat on the right upper limb and shoulder. In the subcutaneous area, there was a decrease in adipose tissue (b), a prominent increase in collagen fibers (c) and dense infiltration of lymphocytes around the vesicles (d). Original magnification: b x50, c x200, d x400.
cde-0007-0070-g01.jpg
PMC4448044_01_cde-0007-0070-g01.jpg
F1
On her initial visit, physical examination revealed multifocal loss of subcutaneous fat on her bilateral upper limbs, shoulders, back, abdomen and left lower limb (fig. 1a). A biopsy specimen from the right shoulder revealed a decrease in adipose tissue (fig. 1b), a prominent increase in collagen fibers (fig. 1c) and dense infiltration of lymphocytes around the vesicles (fig. 1d).
Paraffin-embedded tissue samples from the right shoulder were deparaffinized and stained with anti-CD205 Ab (a), anti-CD163 Ab (b), anti-MMP-7 Ab (c), anti-MMP-28 Ab (d), anti-IL-17 Ab (e) or anti-IL-27 Ab (f). The sections were developed with liquid permanent red. Original magnification: x200.
cde-0007-0070-g02.jpg
PMC4448044_01_cde-0007-0070-g02.jpg
F2
CD205+ matured dendritic cells (fig. 2a), and these cells produced MMP-7 (fig. 2c), MMP-28 (fig. 2d) and IL-27 (fig. 2f). CD163+ macrophages (fig. 2b) and IL-17-producing cells (fig. 2e) were scattered in the same area.