PMC3079022_01
A, gating strategy for FOXP3; CD3+CD4+viable lymphocytes were defined by excluding dead cells using fixable live/dead violet (LDViolet) and a lymphocyte region based on forward and side scatter after backgating on CD3+ cells;. B, FOXP3 versus CD127 (left histograms) and CD25 versus CD127 (right histograms) were used to define the number of regulatory T-cells shown here as blue dots in both data sets. Percentages represent CD3+CD4+CD25+FOXP3+ of CD3+.
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F1
The patient had a significant decrease in his CD4+FOXp3+ T-cells from 13.4% to 3.3% following nine courses of 5-azacitidine (Figure 1).