PMC9719925_01
PMC9719925_02
(A) Photos of the ovary during ovarian tissue biopsy; (B) Ovarian tissue was transported to ovarian tissue cryobank for ovarian tissue preparation; (C) An antral follicle can be seen during ovarian tissue preparation; (D) Detection of follicular activity in ovarian cortex.
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The whole right ovary was removed at Children's Hospital, Capital Institute of Pediatrics, on April 29th, 2022, and normal ovarian morphology could be observed ( Figure 1A ). The ovarian tissue was quickly placed in a low-temperature transfer solution in vitro and transported to our ovarian tissue cryobank within 1 hour ( Figure 1B ). During the preparation of ovarian tissue, the follicles reported by ultrasound were found ( Figure 1C ). The method of follicle counting was the same as the previously published paper, with an average of 1380 follicles in a diameter of 2 mm of the round cortical slice, and the follicle density was about 440/mm3 ( Figure 1D ).
(A, C) The typical pictures of HE staining of the ovarian cortex of this TS girl can see the different stages of follicles, most of which are primordial follicles; (B, D) The typical pictures of HE staining of non-TS girl. Bars: A, B = 50 mum, C, D = 20 mum.
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TS girl can see the different stages of follicles, most of which are primordial ( Figures 2A, C ). The typical HE staining pictures of non-TS girls are shown in  Figures 2B, D .