PMC5512277_01
Follicular conjunctivitis in the conjunctiva tarsi of the patient's left eye.
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PMC5512277_01_fmed-04-00105-g001.jpg
F1
A 29-year-old woman presented to our outpatient clinic with a history of a reddish, swollen left eye for 6 weeks (Figure 1). A follicular inflammation of the conjunctiva tarsi of the upper and lower lid of the left eye as well as a mild conjunctivitis on the right eye was found (Figure 1).
Amplification plots of real-time PCR assays. C. trachomatis PCR (A-D), pan-Chlamydia PCR (E-H), and new pan-Chlamydia/C. felis duplex PCR assay (I-L) on the conjunctival swab from the patient (A,E,I), conjunctival swab from the cat (B,F,J), and throat swab from the cat (C,G,K). (D,H) Positive controls. Results (+, positive; -, negative) are given within the panels for internal control (IC, black), C. trachomatis (Ct, green), pan-Chlamydia PCR (Cs, blue), and the pan-Chlamydia/C. felis duplex PCR (Cs/Cf) containing both the genus-specific (Cs, blue) and the C. felis-specific probe (Cf, red). (L) Specificity controls of the new pan-Chlamydia/C. felis duplex PCR showing positive results with the genus-specific probe (green, black, and blue), but no cross-reactivity of the C. felis-specific probe (red). Three reactions are combined in one panel: DNA from C. psittaci (Cps, cell culture, 105 infection forming units per PCR), C. pneumoniae (Cpn, cell culture, 2 x 104 genomic copies per PCR), and C. trachomatis (Ct, from urethral swab specimen, 4 x 103 infection forming units per PCR).
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PMC5512277_01_fmed-04-00105-g002.jpg
F2
The C. trachomatis-specific PCR was negative, but C. felis-DNA was detected using a pan-Chlamydia PCR/sequencing approach (Figure 2).