PMC4835462_01
Cystoisospora belli oocyst in a direct wet mount smear after staining with modified Ziehl-Neelsen before concentration (Original)
IJPA-11-121-g001.jpg
PMC4835462_01_IJPA-11-121-g001.jpg
F1
Two days later, however, numerous oocysts of C. belli were clearly and frequently seen in a second sample using the Willis flotation method in direct smear and the modified Ziehl-Neelsen staining (Fig. 1).
Gel electrophoresis of PCR product from a Cystoisospora belli infected patient. L. 100-bp DNA ladder. Lane 1. Negative control. Lane 2. 450bp PCR product of Cystoisospora belli
IJPA-11-121-g002.jpg
PMC4835462_01_IJPA-11-121-g002.jpg
F2
For DNA sequencing, PCR products (450 bp) were purified from the agarose gel (Fig. 2), using PCR purification kit (Bioneer, Korea), and sequenced from both directions (Applied Biosystems, DNA Analyzers Sequencing, Bioneer, Korea, Sanger method), using the same primers as those used in primary PCR.