PMC6719345_01
Light microscopy: presence of PAS-positive coagula of cryoglobulin in the glomerular capillaries and arterioles (arrowheads).
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Many glomeruli contained PAS-positive intracapillary coagula of cryoglobulin (Figure 1), and small double contours were observed on silver stain.
Immunofluorescence microscopy: Kappa light chain deposits within the glomerulus. Lambda light chain is negative.
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IF and revealed the following findings: IgM, Kappa, and C3 (3+) granular staining along peripheral capillary loops (Figure 2); IgG and Lambda (negative to trace) staining in the glomeruli. IgA, C1q, and Albumin were negative.
Electron microscopy: subendothelial and subepithelial electron dense deposits of cryoglobulins (arrowheads).
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Electron microscopy (EM): endocapillary proliferation, mesangial interposition, and presence of subendothelial and subepithelial electron dense deposits were observed in the single glomerulus present for electron microscopy (Figure 3).