PMC10337682_01
Images of abdomen computed tomography (CT). Free air was observed around the right retroperitoneal area (indicated by red arrows).
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Abdominal computed tomography (CT) revealed free air around the right retroperitoneal area (Figure 1), which was diagnosed as intestinal perforation.
(A) Weakly gram-positive bacilli were stained by C. ramosum anaerobic bottle culture for 24 hours. (B) The staining of C. ramosum in blood agar for 24 hours was gran-variable/negative. (C) The staining of C. ramosum in blood agar for 48 hours was gram-positive. (D) A pure culture of small, grayish, smooth, round, non-haemolytic colonies with whole edges after 24 hours in blood agar. (E) The colonies of C. ramosum in blood agar for 48 hours were turns to fried egg-shaped, and the color turns to light brown.
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Gram staining revealed a weakly gram-positive rod (Figure 2A). A pure culture of tiny, grayish, smooth, spherical, and non-hemolytic colonies with intact edges was obtained after 24 h (Figure 2D). The colonies of C. ramosum grown in blood agar for 48 h turned egg-shaped, and the color turned light brown (Figure 2E). Gram staining showed gram-variable/negative rods after 24 h (Figure 2B), which became more strongly gram-positive later in culture (Figure 2C).
Recombination-filtered core genome phylogeny for a total of 98 C. ramosum isolates worldwide deposited in the NCBI GenBank database. The isolation date, host, source, and country are represented by squares of different colors. The isolate recovered by this study was highlighted in the red color.
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The phylogenetic relationship between C. ramosum WD-I2 and 97 C. ramosum strains currently deposited in the NCBI GenBank database was investigated to evaluate the genomic epidemiological features of C. ramosum strains in a global context (Figure 3).