We report the case of a 73-year-old who presented with multiple, small, in-transit melanoma nodules located in her right lower extremity below her knee. Positron emission tomography detected two additional non-palpable subcutaneous lesions posterior and medial to the area of previous wide local excision. A novel approach of radioactive seed localization was employed to guide the surgeon to successful surgical resection of all metastatic disease. To our knowledge, the application of radioactive seed localization to melanoma has not been previously reported.