We first describe the application of natural orifice specimen extraction surgery in the treatment of a rectal implantation metastasis tumor from ovarian cancer. One patient diagnosed with recurrent rectal implantation metastasis 1 year after the removal of ovarian cancer successfully underwent transanal specimen extraction via laparoscopic rectectomy without an abdominal incision at the National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College in March 2017. The operation time was 118 minutes, and the intraoperative blood loss was 5 mL. The specimen was extracted via the anus during the operation, and the resection margin was negative. The patient recovered well without complications. Anal function was normal, and the stoma and abdominal incision were well healed after 1 month of follow-up. This study supports the idea that the application of natural orifice specimen extraction surgery for rectal implantation metastasis from ovarian cancer is safe and feasible and can achieve satisfactory outcomes.