Trichoadenoma of Nikolowski is a rare, benign, well differentiated, slowly growing tumor of the hair follicle which was first described in 1958 by Nikolowski. It usually occurs as a solitary nodular lesion between 3 and 15 mm in diameter. It commonly occurs on the face or the buttocks. Herein we report a case of a slowly growing nodular plaque over the vulva of a 60-year-old female, histopathologically proven to be a trichoadenoma. The lesion was completely removed by ablative carbon dioxide laser (CO2).