Platelet-rich fibrin (PRF) has been used for several treatments in dentistry. The present study reports the clinical and radiographic outcomes of a root canal treatment of a necrotic immature maxillary central incisor using PRF. A 15-year-old female patient presented with a diagnosis of maxillary left central incisor pulp necrosis with open apex and periapical radiolucency and extraoral sinus tract. Two months after a two-visit root canal treatment using calcium hydroxide as a root canal dressing, no clinical symptoms were observed, and the previous sinus tract at the patient's nostril had completely disappeared. In the subsequent visit, the PRF was prepared and delivered into the root canal. The PRF layer was covered with collagen membrane and then sealed with white mineral trioxide aggregate. One year later, the patient remained asymptomatic. Radiological examination using cone beam computed tomography (CBCT) showed that the destructive buccal alveolar bone was completely repaired.