An eight-year-old, female intact Golden Retriever underwent magnetic resonance imaging (MRI) for investigation of urinary and faecal incontinence. Soon after induction of general anesthesia, tracheal intubation, and isoflurane administration, hiccup-like movements were evident. These hiccup-like movements did not respond to hyperventilation and increase of anesthetic. After having ruled out pulmonary disease, the animal was reanesthetized with a similar technique; hiccup-like movements reoccurred and did not stop after discontinuation of isoflurane and commencement of a propofol infusion. Eventually, a nondepolarizing neuromuscular blocking agent was administered to stop the hiccup-like response and allow MRI to be performed. This case report describes the pathophysiology of hiccup-like response and its management in a dog.