Emphysematous cystitis is an extremely rare, complicated urinary tract infection with the presence of gas in the bladder wall and lumen caused by gas-producing bacterial infections. A 7-year-old spayed female pomeranian dog was presented with a 3-day history of hematuria and pollakiuria (case 1), and a 9-year-old spayed female jindo dog was presented with a 4-day history of intermittent hematuria (case 2). Imaging modalities, including radiography, ultrasonography, and computed tomography, and bacterial culture tests were used for the diagnosis. Emphysematous cystitis due to Escherichia coli infection with the extension of gas into multiple locations was identified in both cases. Based on the results of antibiotic susceptibility testing, systemic antibiotics were initiated. Both animals had an excellent response to antibiotic treatment, and the clinical signs of the gas collection were completely resolved within ~1 month after treatment initiation. This response was sustained without recurrence in the follow-up period. This case report describes clinical details of extremely rare canine cases of emphysematous cystitis with the extension of gas into multiple locations and evaluates the clinical efficacy of antibiotic therapy.