Aspergillus niger is one of more than 340 different species belonging to the filamentous fungal genus Aspergillus. Aspergillosis can present with a wide range of clinical syndromes, and it results in significant morbidity and mortality worldwide. Here we described a rare case of the left central tympanic membrane perforation secondary to Aspergillus niger otomycosis. The patient underwent tympanoplasty with good improvement.