title: Experiments in Physiology (9th Edition)
author:  Gerald D. Tharp 
description: <P style="MARGIN: 0px"><B>Dr. Gerald D. Tharp</B> is a Professor Emeritus at the University of Nebraska-Lincoln (UNL). He received his Bachelor of Science in Pharmacy from UNL in 1958, his Master of Science in Physiology from UNL in 1961, and his Ph.D. from the University of Iowa in 1965. He has been presented with two Distinguished Teaching awards from UNL and has done extensive research in Exercise Physiology with numerous papers.&nbsp; Dr. Tharp is a fellow of the American College of Sports Medicine.</P> <P style="MARGIN: 0px"> <HR> <P style="MARGIN: 0px"></P> <P style="MARGIN: 0px"><B>Dr. David A. Woodman</B> is a Senior Lecturer in the School of Biological Sciences at the University of Nebraska-Lincoln.&nbsp; He received his Bachelor of Science in Zoology and Botany and his Master of Science in Marine Zoology from Bombay University and his Ph.D. from the University of Nebraska.&nbsp; He has taught General Biology, Ichthyology, Zoology, and Vertebrate Zoology at UNL but since 1990 has been teaching Anatomy and Physiology lectures and supervising the laboratories for these large courses.&nbsp; He is the recipient of a Distinguished Teaching Service Award from the College of Arts and Sciences, and several Outstanding Contributions to Students awards from the UNL Parents Association.</P>
zlib_text: ACKNOWLEDGEMENT
  The author wishes to express his appreciation to 
  Dr. Felix Haurowitz for suggesting this problem and for 
  his helpful guidance and encouragement during this in­
  vestigation.
  He also wishes to thank Raymond Sowinski for his 
  help with the experimental animals, and Margaret Bremer 
  Kennedy for her help in recording data.
  
  The undersigned members of the candidate’s Ph.D. 
  Committee have read and accepted this thesis before it 
  was submitted to the Dean of the Graduate School.
  Dr. Felix Haurowitz
  (ChairraanJ
  Dr. E. E. Campaigne
  Dr. Harry G. Da} 
  M  
  ft
  Dr. T. ¥. Torrey
  Dr. E. Weiss
  Uw.
  
  TABLE OF CONTENTS
  Page
  INTRODUCTION .........................................  1
  EXPERIMENTAL PROCEDURES 
  ............................  11
  I
  I. 
  Preparation of materials
  a. 
  Injection of rabbits......................11
  b. Separation of s e r u m ...................... 12
  1. Rabbit serum
  2. 
  Beef serum
  c. Titration of rabbit s e r u m ............. 14
  d. Preparation of trimethyl(jD-aminophen