title: A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques
author:  Eva Harris 
description: <br>"Makes the polymerase chain reaction (PCR) technique more accessible to researchers, physicians, and laboratory workers throughout the world. Describes the theoretical basis of the technique, practical details, and the philosophy behind the technology transfer program that the author has developed over the past 10 years. A low-cost approach is outlined for use in the study of infectious diseases, which can be applied to other technologies and applications. This approach is especially useful for laboratories in developing countries, and in high school undergraduate and continuing education programs in the US. Appendices give information on construction of equipment, and contain sample charts and worksheets. Includes b&w photos of equipment, and of men and women performing lab procedures. The author is on the faculty of the School of Public Health at UC-Berkeley."--<em>SciTech Book</em> <em>News</em>
zlib_text: Real-time PCR handbook
  
  The image on this cover is of an OpenArray® plate which 
  is primarily used for mid-density real-time PCR on the 
  QuantStudio™ 12K Flex system.  The figure above shows 
  the commonly used formats for real-time PCR.
  Single-tube assays
  96- and 384-well plates
  384-well TaqMan® Array cards
  OpenArray® plates
  
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  Basics of real-time PCR
  Digital PCR
  Experimental design
  Plate preparation
  Data analysis
  Troubleshooting
  Contents
  
  Basics of real-time PCR
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  lifetechnologies.com 
  Basics of real-time PCR
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   1.1  Introduction 
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   1.2  Overview of real-time PCR 
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   1.3  Overview of real-time PCR and  
  real-time PCR components 
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   1.4  Real-time PCR analysis terminology 
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   1.5  Real-time PCR fluorescence detection systems 
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   1.6  Melting curve analysis 
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   1.7  Use of passive reference dyes 
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   1.8  Contamination prevention 
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   1.9  Multiplex real-time PCR 
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   1.10  Internal controls and reference genes 
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   1.11  Real-time PCR instrument calibration 
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