title: Basic Biochemical Laboratory Procedures and Computing: With Principles, Review Questions, Worked Examples, and Spreadsheet Solutions (Topics in Biochemistry)
author:  R. Cecil Jack 
description: <br />"Good review of basic principles and the computer spredsheets are very useful."--Mary Lou Caspers, <em>University of Detroit</em>
zlib_text: C H A P T E R
  10
  Knowledge-Based Simulation 
  of DNA Metabolism:
  Prediction of Action and 
  Envisionment of Pathways
  Adam R. Galper, Douglas L. Brutlag & David H. Millis
  1. Introduction
  Our understanding of any process can be measured by the extent to which
  a simulation we create mimics the real behavior of that process. Deviations
  of a simulation indicate either limitations or errors in our knowledge. In ad-
  dition, these observed differences often suggest veriﬁable experimental hy-
  potheses to extend our knowledge.
  The biochemical approach to understanding biological processes is essen-
  tially one of simulation. A biochemist typically prepares a cell-free extract
  that can mediate a well-described physiological process. The extract is then
  fractionated to purify the components that catalyze individual reactions. Fi-
  
  nally, the physiological process is reconstituted in vitro. The success of the
  biochemical approach is usually measured by how closely the reconstituted
  process matches phy