Hi,
I'm working on plants (Tomato, Arabidopsis) response to salt stress, and i recommand to use 100 mM NaCl for Arabidopsis or it equivalent in PEG. If it is Colombia ecotype, you can get enough shoot fresh matter, while root growth will be very affected. So, you can grow up a high number of plants per treatment to get enough root matter.
Good luck,
---------------------------------------------------------------
Dr. Mohamed Debouba
E-mail : mohamed.debouba from gmail.com
Institut Supérieur de Biologie Appliquée de Médenine
Route El Jorf - Km 22.5 - 4119 Medenine, Tunisie.
Phone : 75 633 919 - Fax : 75 633 918
--- En date de : Mar 26.5.09, arab-gen-request from oat.bio.indiana.edu <arab-gen-request from oat.bio.indiana.edu> a écrit :
De: arab-gen-request from oat.bio.indiana.edu <arab-gen-request from oat.bio.indiana.edu>
Objet: Arab-gen Digest, Vol 49, Issue 9
À: arab-gen from magpie.bio.indiana.edu
Date: Mardi 26 Mai 2009, 19h03
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Today's Topics:
1. PEG8000 use for water deficit (hnbjed)
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Message: 1
Date: Mon, 25 May 2009 00:04:37 -0700 (PDT)
From: hnbjed <hnbjed from yahoo.com>
Subject: [Arabidopsis] PEG8000 use for water deficit
To: arab-gen from magpie.bio.indiana.edu
Message-ID: <164934.51734.qm from web110502.mail.gq1.yahoo.com>
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Hi colleagues,
I want to use PEG8000 to introduce water deficit stress to Arabidopsis roots. What is the suitable concentration of it to induce mild dehydration stress, eg. in 1/2x MS medium? I do not like high concentration as it may cause plasmolysis (I personally do not like 200 or 300 mM NaCl used by so many Arabidopsis researchers in salt stress study). However, a too low concentration of PEG8000 may have slow effect in a short time. Is there anyone knowing a reasonable concentration of it in hydroponics system of Arabidopsis? I want to harvest the tissue in a few hours (less then half a day). How about 10% or 15%? Low concentration stimulates root elongation while high concentration won't, according to some references.
Thanks for your input.
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