Police said the woman was the only person in a northbound vehicle that collided with a car occupied by two adults and two children at about 10:50.
The occupants of the car received treatment for non life-threatening injuries at the scene.
The road remains closed with local diversions in place via Dalwhinnie village.
Police said the identity of the woman would not be released until next of kin had been fully informed.
A woman has died following a crash involving two cars on the A9 at Dalwhinnie in the Highlands.