Two men were attacked in Ivory Blacks in Oswald Street, Glasgow, at about 23:00 on Friday 14 October.
Last month, officers released images of two men they want to speak to in connection with their investigation.
Both men are described as white and aged 25 to 35. The first is 5ft 8in tall with tattoo sleeves, and the other is 6ft 2in with shaved, reddish hair.
Police Scotland said the women may be able to assist them with their inquiries.
Anyone with any information about the incident or who recognises those shown in the images has been urged to contact the police.
A CCTV image of two women who may have witnessed a serious assault at a music venue has been released by police.