The former Army helicopter pilot and two fellow crew members will launch to the International Space Station (ISS) on a landmark flight from Baikonur Cosmodrome, in Kazakhstan.
To mark the launch, #BritinSpace, #Principia - the name of the six-month mission - and #TimPeake are all trending on Twitter.
Here, we feature some of the best of those comments.
@Astro_Matt27 tweets his best wishes to the Chichester-born astronaut:
@mccartanfi tweets her excitement ahead of the launch, scheduled for 10:30 GMT:
@angegarrod and @joannajury are also looking forward to the launch and using #BritinSpace - the highest trending hashtag ahead of the historic spaceflight:
Notable names from the world of sport and TV are also anticipating lift-off, with cricket commentator @Aggerscricket and adventurer @Benfogle adding their best wishes to their fellow countryman:
And just in case he hasn't had time to check the forecast in Kazakhstan for Tuesday, @bbcweather posted this helpful update for the intrepid 43-year-old:
Compiled by Stephen Fottrell.
Maj Tim Peake will become the first British European Space Agency (Esa) astronaut to go into space, on Tuesday.