The Chancellor will meet finance minister Wolfgang Schaeuble to set out more details of the UK's bid to renegotiate its EU membership.
Securing German backing is important for the success of British plans for reform.
David Cameron has already courted support from Chancellor Angela Merkel.
Mr Osborne said: "The UK and German economies are the beating heart of Europe, the engine for growth and jobs.
"Together we make the world's third-largest economy, behind only America and China, and since the crisis ended, we have generated two-thirds of EU growth.
"But the future holds challenges for our economies. We must cut debt and boost productivity. To do this, we need a strong EU, fit for today's challenges and working for the benefit of all 28 member states.
"The UK's reform and renegotiation plans aim to achieve this, which is why these talks with key partners in Europe are so important."
Mr Osborne will also visit a Siemens factory in the German capital and address an audience of business leaders at the annual conference of the BDI, the Federation of German Industries.
In May Germany's Chambers of Commerce and Industry warned that a UK exit from the EU would be "disastrous" for the two countries.
Volker Treier, deputy chief executive of the organisation, told the BBC that German business was "astonished" that the UK was planning a referendum on its EU membership.
David Cameron's renegotiation plans will be formally debated for the first time by the EU's 28 leaders at a summit in Brussels in mid-December.
The British and German economies are the "beating heart" of Europe, George Osborne has said as he heads to Berlin for talks on reforming the EU.