anchor.diff:  	&& make install \
   	&& make clean \
   	&& cd /root ; rm -rf vsearch-2* v2*.tar.gz
  +
  +### install bowtie2 2.3.4.1
  +RUN cd /root \
  +    && wget -O bowtie2-2.3.4.1-linux-x86_64.zip 'https://sourceforge.net/projects/bowtie-bio/files/bowtie2/2.3.4.1/bowtie2-2.3.4.1-linux-x86_64.zip/download' \
  +    && unzip bowtie2-*.zip \
  +    && rm -f bowtie2-*.zip \
  +    && cd bowtie2-* \
  +    && cp bowtie2* /usr/local/bin/.
   
   ### install skewer
   RUN cd /root \
  
positive.code: RUN echo 'DEBIAN_FRONTEND=noninteractive' >> /etc/environment
  
  RUN apt-get update && apt-get install -y \
  	bowtie2 	\
  	cdbfasta 	\
  	cd-hit		\
  	cmake       \
  	dh-autoreconf \
  	git 		\
  	jellyfish 	\
  	libcwd-guard-perl \
  	libberkeleydb-perl \
  	libdata-dump-streamer-perl \
  
negative.code:     && make clean \
      && cd /root ; rm -rf sortmerna-2* 2*.tar.gz
  
  ### install vsearch 2.7.1
  RUN cd /root \
      && wget https://github.com/torognes/vsearch/archive/v2.7.1.tar.gz \
  	&& tar xzf v2*.tar.gz \
  	&& cd vsearch-2* \
  	&& sh ./autogen.sh \
  