anchor.diff:  
   # RUN git clone https://github.com/alaindomissy/pycrispr.git
   # RUN pip install /root/pycrispr/
  +
  +# need this ?
  +#git config --global push.default simple
  
positive.code:   matplotlib==1.5.1 \
    pyqt==4.11.4
  RUN conda install -y -c https://conda.anaconda.org/bioconda pybedtools==0.7.4
  
  COPY files /
  RUN chmod 600 /root/.ssh/id_rsa
  RUN ln -s /RESTORE /BLASTDB && ln -s /RESTORE /PROTOSP
  
  
negative.code: # ubunutu distro baclup and bio cli tools
  RUN apt-get install -y \
    duply \
    libgomp1 \       # needed librery for blast
    ncbi-blast+ \
    bedtools
  
  