anchor.diff:      rm /opt/bwa-${BWA_VERSION}.tar.bz2
   
   # Install SAMTools
  -ENV SAMTOOLS_VERSON="1.4"
  +ENV SAMTOOLS_VERSON="1.6"
   RUN curl -fsSL https://github.com/samtools/samtools/releases/download/${SAMTOOLS_VERSON}/samtools-${SAMTOOLS_VERSON}.tar.bz2 -o /opt/samtools-${SAMTOOLS_VERSON}.tar.bz2 && \
       tar xvjf /opt/samtools-${SAMTOOLS_VERSON}.tar.bz2 -C /opt/ && \
       cd /opt/samtools-${SAMTOOLS_VERSON};make;make install && \
  
positive.code: RUN pip install cutadapt
  
  # Install TrimGalore
  ENV TRIMGALORE_BIN="trim_galore_v0.4.2.zip"
  RUN mkdir /opt/TrimGalore && \
      curl -fsSL http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/$TRIMGALORE_BIN -o /opt/TrimGalore/$TRIMGALORE_BIN && \
      unzip /opt/TrimGalore/$TRIMGALORE_BIN -d /opt/TrimGalore && \
      ln -s /opt/TrimGalore/trim_galore /usr/local/bin/trim_galore && \
      rm /opt/TrimGalore/$TRIMGALORE_BIN
  
  # Install BWA
  ENV BWA_VERSION="0.7.15"
  
negative.code:     rm /opt/${BEDTOOLS_VERSION}.tar.gz
  
  # Install R
  ENV R_VERSION="R-3.4.2"
  RUN curl -fsSL https://cran.r-project.org/src/base/R-3/${R_VERSION}.tar.gz -o /opt/${R_VERSION}.tar.gz && \
      tar xvzf /opt/${R_VERSION}.tar.gz -C /opt/ && \
      cd /opt/${R_VERSION};./configure;make;make install && \
  