anchor.diff:  # Install dependencies from Debian
   #
   RUN apt-get install build-essential hmmer lua5.1 blast2 snap \
  -                    liblua5.1-0 libcairo2 zlib1g libbz2-1.0 libexpat1 libpth20 \
  -                    libncurses5 libsqlite3-0 libpango-1.0-0 \
  -                    libpangocairo-1.0-0 libtre5 python-ctypes liblua5.1-0-dev \
  -                    lua-md5-dev lua-filesystem-dev lua-lpeg-dev libcairo2-dev \
  -                    zlib1g-dev libbz2-dev libexpat1-dev libncurses5-dev \
  -                    libsqlite3-dev libbam-dev libpango1.0-dev libtre-dev \
  -                    python unzip libstorable-perl cpanminus mummer \
  -                    infernal exonerate \
  +                    unzip cpanminus mummer infernal exonerate \
                       --yes --force-yes
   
   #
  
positive.code: # clean up dev stuff (not strictly necessary)
  #
  RUN apt-get purge build-essential --yes --force-yes
  RUN apt-get remove liblua5.1-0-dev lua-md5-dev lua-filesystem-dev lua-lpeg-dev \
                     libcairo2-dev zlib1g-dev libbz2-dev libexpat1-dev \
                     libncurses5-dev libsqlite3-dev libbam-dev libpango1.0-dev \
                     libtre-dev --yes --force-yes
  
  ENV AUGUSTUS_CONFIG_PATH /opt/augustus/config
  ENV RATT_HOME /opt/RATT
  
negative.code: #
  # Add Perl deps (needed for OrthoMCL)
  #
  RUN cpanm --force Carp Storable Bio::SearchIO List::Util Getopt::Long && \
      rm -rf /root/.cpanm/work/
  
  #
  