anchor.diff:  
   ### install vsearch 2.43
   RUN cd /root \
  -        && wget https://github.com/torognes/vsearch/archive/v2.4.3.tar.gz \
  +    && wget https://github.com/torognes/vsearch/archive/v2.4.3.tar.gz \
   	&& tar xzf v2*.tar.gz \
   	&& cd vsearch-2* \
   	&& sh ./autogen.sh \
  
positive.code: 	&& make install \
  	&& make clean \
  	&& cd /root ; rm -rf /root/vsearch-2* 
  ### install CWL runner
  RUN pip install cwlref-runner
  
negative.code: 	&& install -m755 scripts/* /usr/local/bin \
  	&& cd /root ; rm -rf swarm
  
  ### install fastqjoin from ea-utils
  RUN cd /root \
  	&& git clone https://github.com/ExpressionAnalysis/ea-utils.git  \
  	&& cd ea-utils/clipper \
  	&& make \
  	&& install -m755 -s fastq-join /usr/local/bin/ \
  	&& install -m755 -s fastq-multx /usr/local/bin/ \
  	&& cd /root ; rm -rf /root/ea-utils
  
  ### install sortmerna
  RUN cd /root \
  	&& wget https://github.com/biocore/sortmerna/archive/2.1b.tar.gz \
  	&& tar xvf 2.1b.tar.gz \
  