anchor.diff:  RUN pip install --upgrade pip
   
   #########
  -### Create user training
  +### Create user orp
   #########
  -RUN useradd -r -s /bin/bash -U -m -d /home/training -p '' training
  +RUN useradd -r -s /bin/bash -U -m -d /home/orp -p '' orp
   
   #########
   ### Setup the user environment
   ########
  -ENV HOME /home/training
  -RUN usermod -aG sudo,audio,video training \
  +ENV HOME /home/orp
  +RUN usermod -aG sudo,audio,video orp \
   && echo '%sudo ALL=(ALL) NOPASSWD:ALL' >> /etc/sudoers
   
   #########
   ### Oyster River Protocol (ORP)
   #########
  -WORKDIR /home/training
  -USER training
  +WORKDIR /home/orp
  +USER orp
   RUN git clone https://github.com/macmanes-lab/Oyster_River_Protocol.git
   RUN cd Oyster_River_Protocol && sudo make
  -RUN sed -i  "s_ubuntu_training_g" /home/training/Oyster_River_Protocol/software/config.ini
  +RUN sed -i  "s_ubuntu_orp_g" /home/orp/Oyster_River_Protocol/software/config.ini
   
   #########
   ### Path
   #########
  -RUN echo 'PATH=$PATH:/home/training/Oyster_River_Protocol/software/anaconda/install/bin' >> /home/training/.profile
  -RUN echo 'PATH=$PATH:/home/training/Oyster_River_Protocol/software/OrthoFinder/orthofinder' >> /home/training/.profile
  -RUN echo 'PATH=$PATH:/home/training/Oyster_River_Protocol/software/orp-transrate' >> /home/training/.profile
  -RUN echo 'PATH=$PATH:/home/training/Oyster_River_Protocol/software/transabyss' >> /home/training/.profile
  -RUN /bin/bash -c "source /home/training/.profile"
  -ENV PATH="/home/training/Oyster_River_Protocol/software/transabyss:/home/training/Oyster_River_Protocol/software/orp-transrate:/home/training/Oyster_River_Protocol/software/OrthoFinder/orthofinder:/home/training/Oyster_River_Protocol/software/anaconda/install/bin:${PATH}"
  +RUN echo 'PATH=$PATH:/home/orp/Oyster_River_Protocol/software/anaconda/install/bin' >> /home/orp/.profile
  +RUN echo 'PATH=$PATH:/home/orp/Oyster_River_Protocol/software/OrthoFinder/orthofinder' >> /home/orp/.profile
  +RUN echo 'PATH=$PATH:/home/orp/Oyster_River_Protocol/software/orp-transrate' >> /home/orp/.profile
  +RUN echo 'PATH=$PATH:/home/orp/Oyster_River_Protocol/software/transabyss' >> /home/orp/.profile
  +RUN /bin/bash -c "source /home/orp/.profile"
  +ENV PATH="/home/orp/Oyster_River_Protocol/software/transabyss:/home/orp/Oyster_River_Protocol/software/orp-transrate:/home/orp/Oyster_River_Protocol/software/OrthoFinder/orthofinder:/home/orp/Oyster_River_Protocol/software/anaconda/install/bin:${PATH}"
   
   #########
   ### Clean up
  
positive.code: RUN apt update \
      && apt -y upgrade \
      && apt install -y --reinstall language-pack-en \
      && locale-gen en_GB.UTF-8
  RUN apt update && apt install -y build-essential git python python-pip libxml2-dev libz-dev curl wget sudo nano
  
  
  
negative.code: ### Setup the envirenment
  #########
  ENV DEBIAN_FRONTEND noninteractive
  ENV LC_ALL en_US.UTF-8
  ENV LANG en_US.UTF-8
  ENV LANGUAGE en_US:en
  
  #########
  ### Aptitude packages
  