anchor.diff:  ### Setup the envirenment
   #########
   ENV DEBIAN_FRONTEND noninteractive
  -ENV LC_ALL en_GB.UTF-8
  -ENV LANG en_GB.UTF-8
  -ENV LANGUAGE en_GB:en
  +ENV LC_ALL en_US.UTF-8
  +ENV LANG en_US.UTF-8
  +ENV LANGUAGE en_US:en
   
   #########
   ### Aptitude packages
  
positive.code: RUN pip install --upgrade pip
  
  #########
  ### Create user training
  #########
  RUN useradd -r -s /bin/bash -U -m -d /home/training -p '' training
  
  #########
  ### Setup the user environment
  ########
  ENV HOME /home/training
  RUN usermod -aG sudo,audio,video training \
  && echo '%sudo ALL=(ALL) NOPASSWD:ALL' >> /etc/sudoers
  
  #########
  ### Oyster River Protocol (ORP)
  #########
  WORKDIR /home/training
  USER training
  RUN git clone https://github.com/macmanes-lab/Oyster_River_Protocol.git
  RUN cd Oyster_River_Protocol && sudo make
  RUN sed -i  "s_ubuntu_training_g" /home/training/Oyster_River_Protocol/software/config.ini
  
  #########
  ### Path
  #########
  RUN echo 'PATH=$PATH:/home/training/Oyster_River_Protocol/software/anaconda/install/bin' >> /home/training/.profile
  RUN echo 'PATH=$PATH:/home/training/Oyster_River_Protocol/software/OrthoFinder/orthofinder' >> /home/training/.profile
  RUN echo 'PATH=$PATH:/home/training/Oyster_River_Protocol/software/orp-transrate' >> /home/training/.profile
  RUN echo 'PATH=$PATH:/home/training/Oyster_River_Protocol/software/transabyss' >> /home/training/.profile
  RUN /bin/bash -c "source /home/training/.profile"
  ENV PATH="/home/training/Oyster_River_Protocol/software/transabyss:/home/training/Oyster_River_Protocol/software/orp-transrate:/home/training/Oyster_River_Protocol/software/OrthoFinder/orthofinder:/home/training/Oyster_River_Protocol/software/anaconda/install/bin:${PATH}"
  
  #########
  ### Clean up
  
negative.code: ### Setup init workspace
  #########
  WORKDIR $HOME
  USER orp
  RUN /bin/bash -c "source activate orp"
  