anchor.diff:  	&& install -m755 -s fastq-mcf /usr/local/bin \
   	&& cd /root ; rm -rf ea-utils
   
  -### install sortmerna
  +### install sortmerna 2.1b
   RUN cd /root \
   	&& wget https://github.com/biocore/sortmerna/archive/2.1b.tar.gz \
   	&& tar xvf 2*.tar.gz \
  
positive.code: RUN echo 'DEBIAN_FRONTEND=noninteractive' >> /etc/environment
  
  RUN apt-get update && apt-get install -y \
  	bowtie2 	\
  	cdbfasta 	\
  	cd-hit		\
  	cmake       \
  	dh-autoreconf \
  	git 		\
  	jellyfish 	\
  	libcwd-guard-perl \
  	libberkeleydb-perl \
  	libdata-dump-streamer-perl \
  
negative.code:     && make clean \
      && cd /root ; rm -rf sortmerna-2* 2*.tar.gz
  
  ### install vsearch 2.7.1
  RUN cd /root \
      && wget https://github.com/torognes/vsearch/archive/v2.7.1.tar.gz \
  	&& tar xzf v2*.tar.gz \
  	&& cd vsearch-2* \
  	&& sh ./autogen.sh \
  