anchor.diff:  RUN apt-get install -yq --no-install-recommends libbz2-dev
   RUN apt-get install -yq --no-install-recommends unzip
   RUN apt-get install -yq --no-install-recommends libpng-dev
  -RUN DEBIAN_FRONTEND="noninteractive" apt-get install -yq --no-install-recommends tzdata
  +RUN apt-get install -yq --no-install-recommends tzdata
   RUN apt-get install -yq --no-install-recommends r-base
   RUN apt-get install -yq --no-install-recommends libcurl4-openssl-dev
   RUN apt-get install -yq --no-install-recommends libxml2-dev
  
positive.code: RUN apt-key adv --keyserver keyserver.ubuntu.com --recv-keys E298A3A825C0D65DFD57CBB651716619E084DAB9
  RUN apt-get -yq update
  
  # no benefit of combined in builder stage
  RUN apt-get install -yq --no-install-recommends locales
  RUN apt-get install -yq --no-install-recommends g++
  
negative.code: 
  LABEL maintainer="cgphelp@sanger.ac.uk" \
        uk.ac.sanger.cgp="Cancer, Ageing and Somatic Mutation, Wellcome Trust Sanger Institute" \
        version="4.3.4" \
        description="Ascat NGS docker"
  
  RUN apt-get -yq update \
  