anchor.diff:  	&& make install \
   	&& make clean \
   	&& rm -rf /root/vsearch-2.02
  +
  +### install qiime licensed uclust
  +RUN wget -O /usr/local/bin/uclust http://www.drive5.com/uclust/uclustq1.2.22_i86linux64 \
  +    && chmod +x /usr/local/bin/uclust
  
positive.code: 	wget \
  	&& rm -rf /usr/share/doc/ /usr/share/man/ /usr/share/X11/ /usr/share/i18n/ /usr/share/mime /usr/share/locale
  
  
  # ###########
  # copy files into image
  COPY awecmd/* bin/* /usr/local/bin/
  COPY lib/* /usr/local/lib/perl/perl5/
  COPY usearch superblat /usr/local/bin/
  RUN chmod 555 /usr/local/bin/* && strip /usr/local/bin/usearch && strip /usr/local/bin/superblat
  
  
  #### install superblat (from binary in local dir) and BLAT from src
  RUN cd /root \
  
negative.code: 	&& strip /usr/local/bin/blat \
  	&& cd .. \
  	&& rm -rf blatSrc blatSrc35.zip
  
  ### install FragGeneScan from our patched source in github
  RUN cd /root \
  