anchor.diff:  RUN prefetch ERR486835
   RUN prefetch ERR486836
   
  -# Change to directory /root/fastq
  -WORKDIR /root/fastq
  +# Change to directory /data/fastq
  +WORKDIR /data/fastq
   # from within this directory, you can issue command
  -# 'fastq-dump --split-files ../ncbi/public/sra/ERR486835.sra'
  +# 'fastq-dump --split-files /root/ncbi/public/sra/ERR486835.sra'
   # to extract fastq files of accession ERR486835.
  
positive.code: # Download required software
  WORKDIR /root/download
  RUN wget http://spades.bioinf.spbau.ru/release3.10.1/SPAdes-3.10.1-Linux.tar.gz
  # RUN wget http://download.asperasoft.com/download/sw/ascp-client/3.5.4/ascp-install-3.5.4.102989-linux-64.sh
  RUN wget https://ftp-trace.ncbi.nlm.nih.gov/sra/sdk/2.8.2/sratoolkit.2.8.2-ubuntu64.tar.gz
  
  # Install ascp (this is optional for sratoolkit below)
  # RUN chmod u+x ascp-install-3.5.4.102989-linux-64.sh
  # RUN ./ascp-install-3.5.4.102989-linux-64.sh
  
  # Install SPAdes
  WORKDIR /opt
  
negative.code: # Choose OS
  FROM ubuntu:16.10
  
  MAINTAINER Johannes Elias <joheli@gmx.net>
  VOLUME /data
  
  # Install packages
  RUN apt-get -y update && apt-get install -y \
  