anchor.diff:  	| grep tarball_url | cut -f4 -d\" | wget -O download.tar.gz -qi - \
   	&& tar xzfp download.tar.gz \
   	&& rm -f download.tar.gz \
  -  && cd * \
  +  && cd *ea-utils*/clipper \
   	&& make fastq-multx \
   	&& make fastq-join \
   	&& make fastq-mcf \
   	&& install -m755 -s fastq-multx /usr/local/bin \
   	&& install -m755 -s fastq-join /usr/local/bin \
   	&& install -m755 -s fastq-mcf /usr/local/bin \
  -	&& cd /root ; rm -rf ea-utils
  +	&& cd /root \
  +	&& rm -rf *ea-utils*
   
   ### install FragGeneScan from our patched source in github
   RUN cd /root \
  
positive.code: 		| grep tarball_url | cut -f4 -d\" | wget -O download.tar.gz -qi - \
  		&& tar xzfp download.tar.gz \
  		&& rm -f download.tar.gz \
  		&& cd * \
      && make \
      && make install \
      && strip /usr/local/bin/prodigal \
      && make clean \
      && cd /root ; rm -rf Prodigal*
  
  ### install latest sortmerna 2.1b release
  RUN cd /root \
  	&& curl -s https://api.github.com/repos/biocore/sortmerna/releases/latest  \
  	| grep tarball_url | cut -f4 -d\" | wget -O download.tar.gz -qi - \
  	&& tar xzfp download.tar.gz \
  	&& rm -f download.tar.gz \
  	&& cd * \
  	&& sed -i 's/^\#define READLEN [0-9]*/#define READLEN 500000/' include/common.hpp \
  	&& ./configure \
    && make install \
    && make clean \
    && strip /usr/local/bin/sortmerna* \
    && cd /root ; rm -rf sortmerna-2*
  
  ### install skewer
  RUN cd /root \
  
negative.code:     && make \
      && make install \
      && make clean \
      && cd /root \
  		&& rm -rf skewer
  
  ### install latest vsearch release
  RUN cd /root \
  