anchor.diff:                      align = ANCHOR.CENTERLEFT;
                       strand = STRAND.TOP;
                       offset = 0;
  -                    targetTemp = dp.seamlessTm;
  +                    targetTemp = dp.targetTm; // was historically seamlessTm incorrectly till ~ 10/2017
                       sequencePenalties = None}
   
  -                // primercore.oligoDesign false pen task
                   pdWrap false pen task
   
               else
  
positive.code:                      align = ANCHOR.LEFT;
                       strand = STRAND.TOP;
                       offset = 0;
                       targetTemp = dp.seamlessTm;
                       sequencePenalties = None}
                  //primercore.oligoDesign false pen task
                  pdWrap false pen task
  
          /// Called on success at finding fwd and reverse primers that can amplify the adjacent regions
  
negative.code: /// Support routines for primer design scenarios and primer generation for stitches
  open commonTypes
  open System
  open constants // primer parameters
  open Amyris.Bio.primercore
  open Amyris.Bio
  