def blastnprep(self):
        """Setup blastn analyses"""
        # Populate threads for each gene, genome combination
        for sample in self.metadata:
            if sample.general.bestassemblyfile != 'NA':
                #
                # sample[self.analysistype].alleleresults = GenObject()
                sample[self.analysistype].closealleles = dict()
                sample[self.analysistype].mismatches = dict()
                sample[self.analysistype].alignmentlength = dict()
                sample[self.analysistype].subjectlength = dict()
                sample[self.analysistype].queryid = dict()
                sample[self.analysistype].start = dict()
                sample[self.analysistype].end = dict()
                sample[self.analysistype].queryseq = dict()
                if type(sample[self.analysistype].allelenames) == list:
                    for allele in sample[self.analysistype].combinedalleles:
                        # Add each fasta/allele file combination to the threads
                        self.runblast(sample.general.bestassemblyfile, allele, sample)