# Comparison of separation performances of amylose- and cellulose-based stationary phases in the high-performance liquid chromatographic enantioseparation of stereoisomers of β-lactams

Research output: Contribution to journalArticle

8 Citations (Scopus)

### Abstract

High-performance liquid chromatographic methods were developed for the separation of the enantiomers of 19 β-lactams. The direct separations were performed on chiral stationary phases containing either amylose-tris-3,5- dimethylphenyl carbamate, (Kromasil® AmyCoat™ column) or cellulose-tris-3,5-dimethylphenyl carbamate, (Kromasil® CelluCoat™ column) as chiral selector. The different methods were compared in systematic chromatographic examinations. The separations were carried out with good selectivity and resolution. The AmyCoat™ and CelluCoat™ columns appear to be highly complementary. The best separations of bi- and tricyclic b-lactam stereoisomers were obtained with the AmyCoat™ column, whereas the 4-aryl-substituted β-lactams were better separated on the CelluCoat™ column. The elution sequence was determined in all cases; no general rule could be established.

Original language English 120-128 9 Chirality 22 1 https://doi.org/10.1002/chir.20714 Published - Jan 2010

Lactams
Amylose
Stereoisomerism
Cellulose
Liquids
Carbamates
Enantiomers

### Keywords

• β-lactams
• AmyCoat™ column
• CelluCoat™ Column
• Column liquid chromatography

### ASJC Scopus subject areas

• Organic Chemistry
• Analytical Chemistry
• Drug Discovery
• Pharmacology
• Catalysis
• Spectroscopy

### Cite this

In: Chirality, Vol. 22, No. 1, 01.2010, p. 120-128.

Research output: Contribution to journalArticle

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AU - Pataj, Zoltán

AU - Ilisz, I.

AU - Berkecz, R.

AU - Forró, E.

AU - Fülöp, F.

AU - Péter, A.

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N2 - High-performance liquid chromatographic methods were developed for the separation of the enantiomers of 19 β-lactams. The direct separations were performed on chiral stationary phases containing either amylose-tris-3,5- dimethylphenyl carbamate, (Kromasil® AmyCoat™ column) or cellulose-tris-3,5-dimethylphenyl carbamate, (Kromasil® CelluCoat™ column) as chiral selector. The different methods were compared in systematic chromatographic examinations. The separations were carried out with good selectivity and resolution. The AmyCoat™ and CelluCoat™ columns appear to be highly complementary. The best separations of bi- and tricyclic b-lactam stereoisomers were obtained with the AmyCoat™ column, whereas the 4-aryl-substituted β-lactams were better separated on the CelluCoat™ column. The elution sequence was determined in all cases; no general rule could be established.

AB - High-performance liquid chromatographic methods were developed for the separation of the enantiomers of 19 β-lactams. The direct separations were performed on chiral stationary phases containing either amylose-tris-3,5- dimethylphenyl carbamate, (Kromasil® AmyCoat™ column) or cellulose-tris-3,5-dimethylphenyl carbamate, (Kromasil® CelluCoat™ column) as chiral selector. The different methods were compared in systematic chromatographic examinations. The separations were carried out with good selectivity and resolution. The AmyCoat™ and CelluCoat™ columns appear to be highly complementary. The best separations of bi- and tricyclic b-lactam stereoisomers were obtained with the AmyCoat™ column, whereas the 4-aryl-substituted β-lactams were better separated on the CelluCoat™ column. The elution sequence was determined in all cases; no general rule could be established.

KW - β-lactams

KW - AmyCoat™ column

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KW - Column liquid chromatography

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