• 安全检测 •

### HPLC法测定渝东南地区传统土家特色酸鲊肉中的组胺含量

1. 1.重庆三峡医药高等专科学校医学技术系，重庆 404120；2.重庆市抗肿瘤天然药物工程技术研究中心，重庆 404120；3.西南大学食品科学学院，重庆 400715；4.重庆市万州区农产品质量安全监督检测中心，重庆 404120
• 出版日期:2017-06-25 发布日期:2017-06-26
• 基金资助:
重庆市教委科学技术研究项目（KJ1502608）

### Determination of Histamine Content in Suanzharou, a Unique and Traditional Fermented Meat Product of the Tujia Nationality in Southeastern Chongqing by High Performance Liquid Chromatography

RAN Chunxia, CHEN Guangjing, HU Jiang

1. 1. Department of Medical Technology, Chongqing Three Gorges Medical College, Chongqing 404120, China; 2. Chongqing Engineering Research Center of Antitumor Natural Drugs, Chongqing 404120, China; 3. School of Food Science, Univerisity of Southwest, Chongqing 400715, China; 4. Chongqing District of Wanzhou City Agricultural Products Quality Safety Supervision and Inspection Center, Chongqing 404120, China
• Online:2017-06-25 Published:2017-06-26

Abstract: The purpose of this study was to establish a high performance liquid chromatography (HPLC) method for the qualitative and quantitative detection of histamine in Suanzharou, a unique and traditional fermented meat product of the Tujia nationality in southeastern Chongqing. 1,7-Heptanediamine was used as an internal standard substance. Dansyl chloride was used as a derivatization agent. The chromatographic separation was accomplished using an Agilent C18 column as stationary phase and a mixture of 70% methanol and 30% ultra pure water as mobile phase, and the UV detector was set at 254 nm. The analyte was extracted with 0.4 mol/L HClO4 solution and analyzed by HPLC. Results showed that the peaks for 1,7-heptanediamine and histamine dihydrochloride appeared at 4.335 min and 7.723 min, respectively, and an acceptable separation was achieved. The proposed method showed a good linearity between the ratio of peak area (histamine dihydrochloride and 1,7-heptanediamine) and the mass concentration of histamine. The recovery of spiked samples was between 98.5%–102.2%, the instrumental limit of detection (LOD) was 0.50 mg/L and the limit of quantitation (LOQ) was 1.00 mg/L. The precision expressed as relative standard deviation (RSD) was 1.0%. The histamine content of 10 real samples was detected to be in the range of 19.68–44.15 mg/kg, with significant differences existing among these samples (P < 0.05). In conclusion, this method is reliable for the detection of histamine in Suanzharou with high sensitivity and precision.
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