Team:Toulouse/Safety

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How to reduce these Future risks?

How to reduce these Future risks?

We have thought about several ways to minimize risks:

We have thought about several ways to minimize risks:
- - we choose an auxotrophic strain of B. subtilis. The auxotrophy is for the glutamic acid, which is very present in plant's sap.
+ - we choose an auxotrophic strain of B. subtilis. The auxotrophy is for the glutamic acid, which is very present in plant's sap.
- - we choose a non-spore-forming strain of B. subtilis. The sporulation is the endophytic bacteria mechanism used to survive through winter. Thus, our bacterial system will be time limited.
+ - we choose a non-spore-forming strain ofB. subtilis. The sporulation is the endophyte bacteria mechanism used to survive through winter. Thus, our bacterial system will be time limited.
- we want to use a toxin/antitoxin system to avoid gene transfer between our optimized bacterium and another wild-type bacteria.

- we want to use a toxin/antitoxin system to avoid gene transfer between our optimized bacterium and another wild-type bacteria.
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