#### Sample records for 3p cu 3s

1. A study of angle-resolved photoemission extended fine structure as applied to the Ni 3p, Cu 3s, and Cu 3p core levels of the respective clean (111) surfaces

Energy Technology Data Exchange (ETDEWEB)

Huff, W.R.A.; Moler, E.J.; Kellar, S.A. [Lawrence Berkeley National Lab., CA (United States)] [and others

1997-04-01

The first non-s initial state angle-resolved photoemission extended fine structure (ARPEFS) study of clean surfaces for the purpose of further understanding the technique is reported. The surface structure sensitivity of ARPEFS applied to clean surfaces and to arbitrary initial states is studied using normal photoemission data taken from the Ni 3p core levels of a Ni(111) single crystal and the Cu 3s and the Cu 3p core-levels of a Cu(111) single crystal. The Fourier transforms of these clean surface data are dominated by backscattering. Unlike the s initial state data, the p initial state data show a peak in the Fourier transform corresponding to in-plane scattering from the six nearest-neighbors to the emitter. Evidence was seen for single-scattering events from in the same plane as the emitters and double-scattering events. Using a newly developed, multiple-scattering calculation program, ARPEFS data from clean surfaces and from p initial states can be modeled to high precision. Although there are many layers of emitters when measuring photoemission from a clean surface, test calculations show that the ARPEFS signal is dominated by photoemission from atoms in the first two crystal layers. Thus, ARPEFS applied to clean surfaces is sensitive to surface reconstruction. The known contraction of the first two Cu(111) layers is confirmed. The best-fit calculation for clean Ni(111) indicates an expansion of the first two layers. To better understand the ARPEFS technique, the authors studied s and non-s initial state photoemission from clean metal surfaces.

2. Oscillator strength for 3s{sup 2}3p{sup 5}-3s3p{sup 6} in Cl-like ions

Energy Technology Data Exchange (ETDEWEB)

Berrington, K.A.; Waldock, J.A. [School of Science and Mathematics, Sheffield Hallam University, Sheffield (United Kingdom); Pelan, J.C [Gatsby Computational Neuroscience Unit, University College, London (United Kingdom)

2001-07-14

Recent experiments have revealed discrepancies between calculated and experimental lifetimes in the lowest excited level of some chlorine-like ions. New ab initio oscillator strengths using the Breit-Pauli R-matrix method are provided for the 3s{sup 2}3p{sup 5} {sup 2}P{sup o}{sub 3/2,1/2}-3s3p{sup 6} {sup 2}S{sup e}{sub 1/2} transition in Cl-like Ar II through to Cu XIII. Our calculated lifetimes for these twelve ions are respectively 5.507, 2.077, 1.177, 0.784, 0.569, 0.438, 0.350, 0.287, 0.241, 0.206, 0.178 and 0.156 ns, and are more consistent with experiments than other calculations. (author). Letter-to-the-editor.

3. Isotope shifts of the (3s3p) 3P0,1,2-(3s4s) 3S1 Mg I transitions

DEFF Research Database (Denmark)

He, Ming; Therkildsen, Kasper T.; Jensen, Brian B.;

2009-01-01

We report measurements of the isotope shifts of the (3s3p)P30,1,2-(3s4s)S31 Mg I transitions for the stable isotopes M24g (I=0) , M25g (I=5/2) , and M26g (I=0) . Furthermore, the M25g S31 hyperfine coefficient A(S31)=(-321.6±1.5)MHz is extracted and found to be in excellent agreement with state...

4. Measurement of the spin-forbidden decay rate (3s3d)1D2¿(3s3p)3 P2,1 in 24Mg

DEFF Research Database (Denmark)

Therkildsen, K. T.; Jensen, Brian Bak; Ryder, C. P.;

2009-01-01

We have measured the spin-forbidden decay rate from (3s3d)D12¿(3s3p)P32,1 in M24g atoms trapped in a magneto-optical trap. The total decay rate, summing up both exit channels (3s3p)P31 and (3s3p)P32 , yields 196±10s-1 in excellent agreement with resent relativistic many-body calculations of Porsev...

5. Photoionization cross sections of the excited 3s3p 3Po state for atomic Mg

Science.gov (United States)

Wang, Guoli; Wan, Jianjie; Zhou, Xiaoxin

2017-01-01

The photoionization cross sections of the excited levels (3s3p 0,1,2,o 3P) of atomic Mg have been studied theoretically using both the nonrelativistic and fully relativistic R-matrix method. For the threshold cross sections, as previous nonrelativistic studies, present calculations show significant differences (a factor of 3) from former experimental values. Large discrepancies with experiment calls for additional measurements of the photoionization cross sections from the excited states of Mg.

6. Relativistic calculations of 3s2 1S0-3s3p 1P1 and 3s2 1S0-3s3p 3P1,2 transition probabilities in the Mg isoelectronic sequence

Institute of Scientific and Technical Information of China (English)

Cheng Cheng; Gao Xiang; Qing Bo; Zhang Xiao-Le; Li Jia-Ming

2011-01-01

Using the multi-configuration Dirac-Fock self-consistent field method and the relativistic configuration-interaction method, calculations of transition energies, oscillator strengths and rates are performed for the 3s2 1S0-3s3p 1P1 spinallowed transition, 3s2 1S0-3s3p 3P1,2 intercombination and magnetic quadrupole transition in the Mg isoelectronic sequence (Mg Ⅰ, Al Ⅱ, Si ⅢⅢ, P Ⅳ and S Ⅴ). Electron correlations are treated adequately, including intravalence electron correlations. The influence of the Breit interaction on oscillator strengths and transition energies are investigated. Quantum electrodynamics corrections are added as corrections. The calculation results are found to be in good agreement with the experimental data and other theoretical calculations.

7. Measurement of the Hyperfine Structure and Isotope Shifts of the 3s23p2 3P2 to 3s3p3 3Do3 Transition in Silicon

CERN Document Server

Lee, Siu Au

2010-01-01

The hyperfine structure and isotope shifts of the 3s23p2 3P2 to 3s3p3 3Do3 transition in silicon have been measured. The transition at 221.7 nm was studied by laser induced fluorescence in an atomic Si beam. For 29Si, the hyperfine A constant for the 3s23p2 3P2 level was determined to be -160.1+-1.3 MHz (1 sigma error), and the A constant for the 3s3p3 3Do3 level is -532.9+-0.6 MHz. This is the first time that these constants were measured. The isotope shifts (relative to the abundant isotope 28Si) of the transition were determined to be 1753.3+-1.1 MHz for 29Si and 3359.9+-0.6 MHz for 30Si. This is an improvement by about two orders of magnitude over a previous measurement. From these results we are able to predict the hyperfine structure and isotope shift of the radioactive 31Si atom, which is of interest in building a scalable quantum computer.

8. Radiative rates for E1, E2, M1, and M2 transitions among the 3s$^2$3p$^5$, 3s3p$^6$, and 3s$^2$3p$^4$3d configurations of Cl-like W LVIII

CERN Document Server

Aggarwal, K M

2014-01-01

We report calculations of energy levels, radiative decay rates, and lifetimes for transitions among the 3s$^2$3p$^5$, 3s3p$^6$, and 3s$^2$3p$^4$3d configurations of Cl-like W LVIII. The general-purpose relativistic atomic structure package (GRASP) has been adopted for our calculations. Comparisons are made with the most recent results of Mohan et al. [Can. J. Phys. {\\bf 92} (2014) xxx] and discrepancies in lifetimes are noted, up to four orders of magnitude in some instances. Our energy levels are estimated to be accurate to better than 0.5\\%, whereas results for radiative rates and lifetimes should be accurate to better than 20\\%.

9. The temperature dependence of the cross section for the energy pooling process Na(3P)+Na(3P) to Na(4D)+Na(3S)

Science.gov (United States)

Horvatic, V.; Movre, M.; Vadla, C.

1999-10-01

We report the measurements of the temperature dependence of the cross section σ4D for the energy pooling process Na(3P)+Na(3P) to Na(4D)+Na(3S). The latest two, as yet undisputed, results for σ4D obtained by different authors at T = 597 K and T = 483 K suggest that this cross section decreases with increasing T, which contradicts the theory and other experiments on similar processes. To resolve this controversy and to examine the temperature trend of the cross section, we have measured the σ4D in the temperature range 567-705 K, covering the high-temperature region that has not yet been investigated experimentally. To determine σ4D we have excited sodium atoms in the quasistatic wing of the D1 line using a cw dye laser and measured the fluorescence intensity for the 4D to 3P3/2 transition, relative to the intensity of the optically thin quasistatic wing of the D2 line. The spatial distribution of the number density of the sodium atoms in the 3P3/2 state and the sodium ground-state number density were measured too. The method used for the determination of the cross section is advantageous since it entirely circumvents the need to account for the radiation trapping of 3P level radiation, which was substantial under experimental conditions of the ground-state densities being 1014-1016 cm-3. The measurements of the cross section σ4D in the investigated temperature range have shown that it increases as ~exp(-Δ E/kT). From the experiment we obtained Δ E = (608±95) cm-1, which is in excellent agreement with the energy defect (613 cm-1) for the considered process, and in fair agreement with the values which follow from recent theoretical calculations.

10. Atomic scattering in the diffraction limit: electron transfer in keV Li+-Na(3s, 3p) collisions

DEFF Research Database (Denmark)

Poel, Mike van der; Nielsen, C.V.; Rybaltover, M.;

2002-01-01

. This setup yields a momentum resolution of 0.12 an, an order of magnitude better angular resolution than previous measurements on this system. This enables us to clearly resolve Fraunhofer-type diffraction patterns in the angle DCS. In particular, the angular width of the ring structure is given by the ratio...... of the de Broglie wavelength lambda(dB) = 150 fm at a velocity v = 0.20 au and the effective atomic diameter for electron capture 2R = 20 au. Parallel AO and MO semiclassical coupled-channel calculations of the Na(3s, 3p) --> Li(2s, 2p) state-to-state collision amplitudes have been performed, and quantum...

11. Cu3P/RGO Nanocomposite as a New Anode for Lithium-Ion Batteries

Science.gov (United States)

Liu, Shuling; He, Xiaodong; Zhu, Jianping; Xu, Liqiang; Tong, Jianbo

2016-10-01

Cu3P/reduced graphene oxide (Cu3P/RGO) nanocomposite was successfully synthesized by a facile one-pot method as an advanced anode material for high-performance lithium-ion batteries. Cu3P nanostructures with a polyhedral shape with the mean diameter (80–100 nm) were homogeneously anchored on the surface of RGO. The flexible RGO sheets acted as elastic buffering layer which not only reduced the volume change, but also prevented the aggregation of Cu3P nanostructures, the cracking and crumbing of electrodes. On the other hand, the presence of Cu3P nanostructures could also avoid the agglomeration of RGO sheets and retain their highly active surface area. Therefore, as an advanced anode material for high-performance lithium-ion batteries, the as-prepared Cu3P/RGO exhibited high capacity of 756.15 mAhg‑1 at the current density 500 mAg‑1 after 80 cycles, superior cyclic stability and good rate capability.

12. Photoionisation of Cl+ from the 3s23p4 3P2, 1, 0 and the 3s23p4 1D2, 1S0 states in the energy range 19 - 28 eV

Science.gov (United States)

McLaughlin, Brendan M.

2016-10-01

Absolute photoionisation cross sections for the Cl+ ion in its ground and the metastable states; 3s23p4 3P2, 1, 0, and 3s23p4 1D2, 1S0, were measured recently at the Advanced Light Source (ALS) at Lawrence Berkeley National Laboratory using the merged beams photon-ion technique at an photon energy resolution of 15 meV in the energy range 19 - 28 eV. These measurements are compared with large-scale Dirac Coulomb R-matrix calculations in the same energy range. Photoionisation of this sulphur-like chlorine ion is characterized by multiple Rydberg series of autoionizing resonances superimposed on a direct photoionisation continuum. A wealth of resonance features observed in the experimental spectra are spectroscopically assigned and their resonance parameters tabulated and compared with the recent measurements. Metastable fractions in the parent ion beam are determined from the present study. Theoretical resonance energies and quantum defects of the prominent Rydberg series 3s23p3nd, identified in the spectra as 3p → nd transitions are compared with the available measurements made on this element. Weaker Rydberg series 3s23p3ns, identified as 3p → ns transitions and window resonances 3s3p4(4P)np features, due to 3s → np transitions are also found in the spectra.

13. Experimental Determination of the 24Mg I (3s3p)3P2 Lifetime

DEFF Research Database (Denmark)

Jensen, Brian Bak; He, Ming; Westergaard, Philip Grabow;

2011-01-01

We present the first experimental determination of the electric-dipole forbidden (3s3p)3P2¿(3s2)1S0 (M2) transition rate in 24Mg and compare to state-of-the-art theoretical predictions. Our measurement exploits a magnetic trap isolating the sample from perturbations and a magneto-optical trap...... as an amplifier converting each 3P2¿1S0 decay event into millions of photons readily detected. The transition rate is determined to be (4.87±0.3)×10-4¿¿s-1 corresponding to a 3P2 lifetime of 2050-110+140 sec. This value is in agreement with recent theoretical predictions, and to our knowledge the longest lifetime...

14. Auger Spectra and Different Ionic Charges Following 3s, 3p and 3d Sub-Shells Photoionization of Kr Atoms

Directory of Open Access Journals (Sweden)

Yehia A. Lotfy

2006-01-01

Full Text Available The decay of inner-shell vacancy in an atom through radiative and non-radiative transitions leads to final charged ions. The de-excitation decay of 3s, 3p and 3d vacancies in Kr atoms are calculated using Monte-Carlo simulation method. The vacancy cascade pathway resulted from the de-excitation decay of deep core hole in 3s subshell in Kr atoms is discussed. The generation of spectator vacancies during the vacancy cascade development gives rise to Auger satellite spectra. The last transitions of the de-excitation decay of 3s, 3p and 3d holes lead to specific charged ions. Dirac-Fock-Slater wave functions are adapted to calculate radiative and non-radiative transition probabilities. The intensity of Kr^{4+} ions are high for 3s hole state, whereas Kr^{3+} and Kr^{2+} ions have highest intensities for 3p and 3d hole states, respectively. The present results of ion charge state distributions agree well with the experimental data.

15. Measurement of photoionization cross section from the 3s3p {sup 1}P{sub 1} excited state of magnesium

Energy Technology Data Exchange (ETDEWEB)

Rafiq, M; Hussain, Shahid; Saleem, M; Kalyar, M A; Baig, M A [Department of Physics, Atomic and Molecular Physics Laboratory, Quaid-i-Azam University, Islamabad (Pakistan)

2007-06-28

The photoionization cross section from the 3s3p {sup 1}P{sub 1} excited state has been measured in the energy region from the first ionization threshold up to 1.4 eV excess energy using a two-step photoionization and saturated ionization technique in conjunction with an atomic beam source and a time-of-flight mass spectrometer that enables the separation of the three stable isotopes of magnesium on the time axis. The absolute value of the photoionization cross sections from the 3s3p {sup 1}P{sub 1} excited state near the 3s ionization threshold has been measured as 90 {+-} 16 Mb (at 354.5 nm ionizing wavelength) for the dominating isotope ({sup 24}Mg) whereas the value at the peak of the 3p{sup 21}S{sub 0} auto-ionizing resonance has been determined as 785 {+-} 141 Mb. The present experimentally measured photoionization cross sections are compared with the existing experimental and theoretical work, showing excellent agreement.

16. Characterization of Cu3P phase in Sn3.0Ag0.5Cu0.5P/Cu solder joints

Institute of Scientific and Technical Information of China (English)

Jian-xun Chen; Xing-ke Zhao; Xu-chen Zou; Ji-hua Huang; Hai-chun Hu; Hai-lian Luo

2014-01-01

This article reports the effects of phosphorus addition on the melting behavior, microstructure, and mechanical properties of Sn3.0Ag0.5Cu solder. The melting behavior of the solder alloys was determined by differential scanning calorimetry. The interfacial micro-structure and phase composition of solder/Cu joints were studied by scanning electron microscopy and energy dispersive spectrometry. Thermodynamics of Cu-P phase formation at the interface between Sn3.0Ag0.5Cu0.5P solder and the Cu substrate was characterized. The results indicate that P addition into Sn3.0Ag0.5Cu solder can change the microstructure and cause the appearance of rod-like Cu3P phase which is distributed randomly in the solder bulk. The Sn3.0Ag0.5Cu0.5P joint shows a mixture of ductile and brittle fracture after shear test-ing. Meanwhile, the solidus temperature of Sn3.0Ag0.5Cu solder is slightly enhanced with P addition.

17. The Cu{sub 2}FeTi{sub 3}S{sub 8} and Cu{sub 2}FeZr{sub 3}S{sub 8} compounds: Crystal structure and electroanalytical application

Energy Technology Data Exchange (ETDEWEB)

Kormosh, Zh., E-mail: kormosh@univer.lutsk.ua [Department of Analytical Chemistry and Eco-technology, Volyn National University, 13 Voli Ave, 43025 Lutsk (Ukraine); Fedorchuk, A. [Department of Inorganic and Organic Chemistry, Lviv National University of Veterinary Medicine and Biotechnologies, 50 Pekarska St., 79010 Lviv (Ukraine); Wojciechowski, K. [Department of Inorganic Chemistry, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Cracow (Poland); Tataryn, N. [Department of Analytical Chemistry and Eco-technology, Volyn National University, 13 Voli Ave, 43025 Lutsk (Ukraine); Parasyuk, O. [Department of Inorganic and Physical Chemistry, Volyn National University, 13 Voli Ave, 43025 Lutsk (Ukraine)

2011-04-08

The Cu{sub 2}FeTi{sub 3}S{sub 8} and Cu{sub 2}FeZr{sub 3}S{sub 8} compounds were produced by solid-state synthesis. The crystal structure of the quaternary phases was investigated by X-ray powder method. The compounds are described in the thiospinel structure (space group Fd3-bar m) with the unit cell parameters a = 1.00099(1) nm (Cu{sub 2}FeTi{sub 3}S{sub 8}) and a = 1.03837(2) nm (Cu{sub 2}FeZr{sub 3}S{sub 8}). The atomic parameters were calculated in the isotropic approximation (R{sub I} = 0.0496 and R{sub I} = 0.0422 for Cu{sub 2}FeTi{sub 3}S{sub 8} and Cu{sub 2}FeZr{sub 3}S{sub 8}, respectively). Iron(III)-selective electrodes were prepared using the chalcogenide compounds Cu{sub 2}FeTi{sub 3}S{sub 8} and Cu{sub 2}FeZr{sub 3}S{sub 8}. The electrode function slopes are 52.7 mV/pC for Cu{sub 2}FeTi{sub 3}S{sub 8} and 66.2 mV/pC for Cu{sub 2}FeZr{sub 3}S{sub 8}, the detection limits are 1 x 10{sup -5} M and 2 x 10{sup -5} M respectively. The prepared electrochemical sensors are not sensitive to Cd{sup 2+}, Zn{sup 2+}, Mn{sup 2+}, Ni{sup 2+}, Co{sup 2+}, Mg{sup 2+}, Cr{sup 3+}, and Fe{sup 2+} ions, and were tested in the potentiometric titration of Fe(III) with EDTA solution.

18. Electronic, Magnetic, and Redox Properties of [MFe(3)S(4)] Clusters (M = Cd, Cu, Cr) in Pyrococcus furiosus Ferredoxin.

Science.gov (United States)

Staples, Christopher R.; Dhawan, Ish K.; Finnegan, Michael G.; Dwinell, Derek A.; Zhou, Zhi Hao; Huang, Heshu; Verhagen, Marc F. J. M.; Adams, Michael W. W.; Johnson, Michael K.

1997-12-01

The ground- and excited-state properties of heterometallic [CuFe(3)S(4)](2+,+), [CdFe(3)S(4)](2+,+), and [CrFe(3)S(4)](2+,+) cubane clusters assembled in Pyrococcus furiosus ferredoxin have been investigated by the combination of EPR and variable-temperature/variable-field magnetic circular dichroism (MCD) studies. The results indicate Cd(2+) incorporation into [Fe(3)S(4)](0,-) cluster fragments to yield S = 2 [CdFe(3)S(4)](2+) and S = (5)/(2) [CdFe(3)S(4)](+) clusters and Cu(+) incorporation into [Fe(3)S(4)](+,0) cluster fragments to yield S = (1)/(2) [CuFe(3)S(4)](2+) and S = 2 [CuFe(3)S(4)](+) clusters. This is the first report of the preparation of cubane type [CrFe(3)S(4)](2+,+) clusters, and the combination of EPR and MCD results indicates S = 0 and S = (3)/(2) ground states for the oxidized and reduced forms, respectively. Midpoint potentials for the [CdFe(3)S(4)](2+,+), [CrFe(3)S(4)](2+,+), and [CuFe(3)S(4)](2+,+) couples, E(m) = -470 +/- 15, -440 +/- 10, and +190 +/- 10 mV (vs NHE), respectively, were determined by EPR-monitored redox titrations or direct electrochemistry at a glassy carbon electrode. The trends in redox potential, ground-state spin, and electron delocalization of [MFe(3)S(4)](2+,+) clusters in P. furiosus ferredoxin are discussed as a function of heterometal (M = Cr, Mn, Fe, Co, Ni, Cu, Zn, Cd, and Tl).

19. 类镁离子3s2 1S0-3s3p 3P1(Z=14～103)跃迁的相对论多组态研究%Spin-forbidden Transitions for Mg-like Ions(Z＝14～103)

Institute of Scientific and Technical Information of China (English)

易有根; 郑志坚; 唐永建; 朱正和

2003-01-01

利用全相对论性多组态Dirac-Fock平均能级(MCDF-AL)方法系统计算了高剥离类镁离子互组合线3s2 1S0-3s3p 3P1(Z=14～103)光谱跃迁的能级间隔和跃迁几率.计算中考虑了重要核的有限体积效应及Breit修正和QED修正,所得结果和最近的实验数据及理论计算值进行了比较.结果表明,高原子序数的高荷电离子跃迁的互组合线跃迁几率和中性原子的电偶极E1的相当.在ICF和MCF高温高密度激光等离子体中,互组合线跃迁几率过程不容被忽视.

20. Determination and analysis of dispersive optical constants of CuIn3S5 thin films

Science.gov (United States)

Khemiri, N.; Sinaoui, A.; Kanzari, M.

2011-04-01

CuIn3S5 thin films were prepared from powder by thermal evaporation under vacuum (10-6 mbar) onto glass substrates. The glass substrates were heated from 30 to 200 °C. The films were characterized for their optical properties using optical measurement techniques (transmittance and reflectance). We have determined the energy and nature of the optical transitions of films. The optical constants of the deposited films were determined in the spectral range 300-1800 nm from the analysis of transmission and reflection data. The Swanepoel envelope method was employed on the interference fringes of transmittance patterns for the determination of variation of refractive index with wavelength. Wemple-Di Domenico single oscillator model was applied to determine the optical constants such as oscillator energy E0 and dispersion energy Ed of the films deposited at different substrate temperatures. The electric free carrier susceptibility and the ratio of the carrier concentration to the effective mass were estimated according to the model of Spitzer and Fan.

1. Photoionization of Cl+ from the 3s23p4 3P2,1,0 and the 3s23p4 1D2,1S0 states in the energy range 19-28 eV

Science.gov (United States)

McLaughlin, Brendan M.

2017-01-01

Absolute photoionization cross-sections for the Cl+ ion in its ground and the metastable states, 3s23p4 3P2,1,0 and 3s23p4 1D2,1S0, were measured recently at the Advanced Light Source at Lawrence Berkeley National Laboratory using the merged beams photon-ion technique at a photon energy resolution of 15 meV in the energy range 19-28 eV. These measurements are compared with large-scale Dirac-Coulomb R-matrix calculations in the same energy range. Photoionization of this sulphur-like chlorine ion is characterized by multiple Rydberg series of auto-ionizing resonances superimposed on a direct photoionization continuum. A wealth of resonance features observed in the experimental spectra is spectroscopically assigned, and their resonance parameters are tabulated and compared with the recent measurements. Metastable fractions in the parent ion beam are determined from this study. Theoretical resonance energies and quantum defects of the prominent Rydberg series 3s23p3nd, identified in the spectra as 3p → nd transitions, are compared with the available measurements made on this element. Weaker Rydberg series 3s23p3ns, identified as 3p → ns transitions and window resonances 3s3p4(4P)np features, due to 3s → np transitions, are also found in the spectra.

2. Comparative study of structural and morphological properties of CuIn{sub 3}S{sub 5} and CuIn{sub 7}S{sub 11} materials

Energy Technology Data Exchange (ETDEWEB)

Khemiri, N., E-mail: naoufel_khemiri@yahoo.f [Laboratoire de Photovoltaique et Materiaux Semi-conducteurs, ENIT BP 37, Le belvedere 1002-Tunis (Tunisia); Kanzari, M. [Laboratoire de Photovoltaique et Materiaux Semi-conducteurs, ENIT BP 37, Le belvedere 1002-Tunis (Tunisia)

2010-02-15

CuIn{sub 3}S{sub 5} and CuIn{sub 7}S{sub 11} powders were prepared by solid-state reaction method using high-purity elemental copper, indium and sulphur. The films prepared from CuIn{sub 3}S{sub 5} and CuIn{sub 7}S{sub 11} powders were grown by thermal evaporation under vacuum (10{sup -6} Torr) on glass substrates at different substrate temperature Ts varying from room temperature to 200 deg. C. The powders and thin films were characterized for their structural properties by using X-ray diffraction (XRD) and energy dispersive X-ray (EDX). Both powders were polycrystalline with chalcopyrite and spinel structure, respectively. From the XRD data, we calculated the lattice parameters of the structure for the compounds. For CuIn{sub 3}S{sub 5} powder, we also calculated the cation-anion bond lengths. The effect of substrate temperature Ts on the structural properties of the films, such as crystal phase, preferred orientation and crystallinity was investigated. Indeed, X-ray diffraction analysis revealed that the films deposited at a room temperature (30 deg. C) are amorphous in nature while those deposited on heated were polycrystalline with a preferred orientation along (1 1 2) of the chalcopyrite phase and (3 1 1) of the spinel phase for CuIn{sub 3}S{sub 5} and CuIn{sub 7}S{sub 11} films prepared from powders, respectively. The morphology of the films was determined by atomic force microscopy AFM. The surface roughness and the grain size of the films increase on increasing the substrate temperature.

3. A vibrational and TDS study of sulfur adsorbates on Cu(100): Evidence for CH 3S species

Science.gov (United States)

Sexton, B. A.; Nyberg, G. L.

1986-01-01

Vibrational (EELS) and TDS data for methyl mercaptan (CH 3SH), dimethyl sulfide (CH 3) 2S and dimethyl disulfide (CH 3S) 2 are analyzed to determine the nature of the adsorption states on Cu(100). Dimethyl sulfide is reversibly adsorbed on Cu(100); no dissociation (CS bond breaking) was found. By contrast, methyl mercaptan and dimethyl disulfide dissociate below 300 K to form adsorbed CH 3S (methyl mercaptide) species. Depending on the coverage, two orientations of methyl mercaptide are found: linear and bent. The two different orientations can be distinguished via the surface dipole selection rule by different intensities of the methyl rocking and deformation vibrations. By contrast with the methoxy species, which on Cu(100) decomposes to formaldehyde, no H 2C=S is liberated during decomposition of CH 3S. The mercaptide is stable to ˜ 350 K, but decomposes at higher temperatures to form adsorbed sulfur and recombinant methane, hydrogen and ethane. The methane appears to be formed by methyl-hydrogen recombination when the C-S bond scission occurs. TDS results show that sulfur released from the decomposition poisons the surface toward further adsorption. In addition, the selectivity toward methane versus ethane can be altered by pre-titrating the adsorbed hydrogen with oxygen, thereby changing the relative methyl-hydrogen and methyl-methyl recombination probabilities.

4. Nano-sized quaternary CuGa2In3S8 as an efficient photocatalyst for solar hydrogen production

KAUST Repository

Kandiel, Tarek

2014-09-03

The synthesis of quaternary metal sulfide (QMS) nanocrystals is challenging because of the difficulty to control their stoichiometry and phase structure. Herein, quaternary CuGa2In3S8 photocatalysts with a primary particle size of ≈4nm are synthesized using a facile hot-injection method by fine-tuning the sulfur source injection temperature and aging time. Characterization of the samples reveals that quaternary CuGa2In3S8 nanocrystals exhibit n-type semiconductor characteristics with a transition band gap of ≈1.8eV. Their flatband potential is located at -0.56V versus the standard hydrogen electrode at pH6.0 and is shifted cathodically by 0.75V in solutions with pH values greater than 12.0. Under optimized conditions, the 1.0wt% Ru-loaded CuGa2In3S8 photocatalyst exhibits a photocatalytic H2 evolution response up to 700nm and an apparent quantum efficiency of (6.9±0.5)% at 560nm. These results indicate clearly that QMS nanocrystals have great potential as nano-photocatalysts for solar H2 production. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

5. Photoionisation of Cl$^+$ from the $3s^23p^4\\;^3P_{2,1,0}$ and the$3s^23p^4\\;^1D_2, ^1S_0$ states in the energy range 19 - 28 eV

CERN Document Server

McLaughlin, Brendan M

2016-01-01

Absolute photoionisation cross sections for the Cl$^+$ ion in its ground and the metastable states; $3s^2 3p^4\\; ^3P_{2,1,0}$, and $3s^2 3p^4\\; ^1D_2,\\; ^1S_0$, were measured recently at the Advanced Light Source ALS) at Lawrence Berkeley National Laboratory using the merged beams photon-ion technique at an photon energy resolution of 15 meV in the energy range 19 -- 28 eV. These measurements are compared with large-scale Dirac Coulomb {\\it R}-matrix calculations in the same energy range. Photoionisation of this sulphur-like chlorine ion is characterized by multiple Rydberg series of autoionizing resonances superimposed on a direct photoionisation continuum. A wealth of resonance features observed in the experimental spectra are spectroscopically assigned and their resonance parameters tabulated and compared with the recent measurements. Metastable fractions in the parent ion beam are determined from the present study. Theoretical resonance energies and quantum defects of the prominent Rydberg series $3s^2 3p... 6. Diffusion Brazing of Al6061/15 Vol. Pct Al2O3p Using a Cu-Sn Interlayer Science.gov (United States) Cooke, Kavian O.; Khan, Tahir I.; Oliver, Gossett D. 2013-06-01 Diffusion brazing of Al-6061 alloy containing 15 vol. pct Al2O3 particles was attempted using Cu-Sn interlayer. Joint formation was attributed to the solid-state interdiffusion of Cu and Sn followed by eutectic formation and subsequent isothermal solidification. Examination of the joint region using scanning electron microprobe analyzer (EPMA), wavelength dispersive spectroscopy (WDS) and X-ray diffraction (XRD) showed the formation of intermetallic phases such as Al7Cu3Mg3, Mg2Cu6Al5, Cu3Sn, and Mg2Sn. The results indicated an increase in joint strength with increasing bonding time giving the highest joint shear strength of 94 MPa at a bonding duration of 3 hours. 7. Characteristics of CuO-MoO3-P2O5 catalyst and its catalytic wet oxidation (CWO) of dye wastewater under extremely mild conditions. Science.gov (United States) Ma, Hongzhu; Zhuo, Qiongfang; Wang, Bo 2007-11-01 In order to develop a catalyst with high activity for catalytic wet oxidation (CWO) processing at lower temperatures (35 degrees C) and atmospheric pressure, a new CuO-MoO3-P2O5 catalystwas synthesized by a solid-state reaction method and was characterized by X-ray diffraction (XRD), Fourier transformation infrared spectrometer (FTIR), X-ray photoelectron spectroscopy (XPS), selected area electronic diffraction (SAED), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDXS) for elemental mapping. Methylene blue (MB) was adopted to investigate the catalytic activity of CuO-MoO3-P2O5 in CWO processing. The results show that this new catalyst has a high catalytic activity to decolorize MB under mild condition. The color removal of MB (the initial concentration was 0.3 g L(-1) and initial pH was 5) can reach to 99.26% within 10 min at 35 degrees C and atmospheric pressure. Catalyst lifespan and selectivity were also tested, and the results show that after the catalyst was used three times, catalyst activity still remains. Selectivity testing shows that CuO-MoO3-P2O5 has high catalytic activity on degradation of MB, whereas this catalyst has less impact on methyl orange (the color removal was 99.65% for MB and 55% for methyl orange under the same conditions). According to the experimental results, a possible mechanism of catalytic degradation of MB was proposed. 8. Study of χ{sub b} meson production in pp collisions at √(s) = 7 and 8 TeV and observation of the decay χ{sub b} (3P) → Υ (3S Energy Technology Data Exchange (ETDEWEB) Aaij, R. [Nikhef National Institute for Subatomic Physics, Amsterdam (Netherlands); Adeva, B. [Universidad de Santiago de Compostela (Spain); Adinolfi, M. [Bristol Univ. (United Kingdom). H.H. Wills Physics Lab.; and others 2014-10-15 A study of χ{sub b} meson production at LHCb is performed on proton-proton collision data, corresponding to 3.0 fb{sup -1} of integrated luminosity collected at centre-of-mass energies √(s) = 7 and 8 TeV. The fraction of Υ(nS) mesons originating from χ{sub b} decays is measured as a function of the Υ transverse momentum in the rapidity range 2.0 < y{sup Υ} < 4.5. The radiative transition of the χ{sub b} (3P) meson to Υ(3S) is observed for the first time. The χ{sub b1} (3P) mass is determined to be m{sub χ{sub b{sub 1{sub ({sub 3{sub P{sub )}}}}}}} = 10 511.3 ± 1.7 ± 2.5MeV/c{sup 2}, where the first uncertainty is statistical and the second is systematic. (orig.) 9. Heterospin systems constructed from [Cu2Ln]3+ and [Ni(mnt)2]1-,2- Tectons: First 3p-3d-4f complexes (mnt = maleonitriledithiolato). Science.gov (United States) Madalan, Augustin M; Avarvari, Narcis; Fourmigué, Marc; Clérac, Rodolphe; Chibotaru, Liviu F; Clima, Sergiu; Andruh, Marius 2008-02-04 Cu ion from the other unit. The magnetic properties of the gadolinium-containing complexes have been determined. Ferromagnetic exchange interactions within the trinuclear [Cu(2)Gd] motifs occur. In the compounds 4 and 7, the [Ni(mnt)(2)](*-) radical anions contribution to the magnetization is clearly observed in the high-temperature regime, and most of it vanishes upon temperature decrease, very likely because of the rather strong antiferromagnetic exchange interactions between the open-shell species. The extent of the exchange interaction in the compound 7, which was found to be antiferromagnetic, between the coordinated Cu center and the corresponding [Ni(mnt)(2)](*-) radical anion, bearing mostly a 3p spin type, was estimated through CASSCF/CASPT2 calculations. Compound 6 exhibits a slow relaxation of the magnetization. 10. Effects of Mg content on aging behavior of sub-micron Al2O3p/Al-Cu-Mg composites Institute of Scientific and Technical Information of China (English) JIANG Long-tao; ZHU De-zhi; CHEN Guo-qin; XIU Zi-yang; WU Gao-hui 2006-01-01 30%Al2O3p/Al-Cu-2.0Mg composite and Al2O3p/Al-Cu-2.5Mg composite with 0.3 μm-Al2O3 particles were fabricated.Age-hardening behaviors of two composites and the related matrix alloys were studied by means of Brinell-hardness measurement,DSC and TEM. The results show that the hardness of the composite is improved obviously because of the addition of sub-micron Al2O3 particles. But the hardness increment of Al2O3p/Al composite after aging is lower than that of the related matrix alloy.Moreover, the formation of GP region is suppressed by the addition of sub-micron Al2O3 particles, which broadens the exothermic peak of S' phase. The increment of Mg content has a different influence on accelerating the aging processes of aluminum alloys and the composites, and the hardness also increases. 11. Determination and analysis of dispersive optical constants of CuIn{sub 3}S{sub 5} thin films Energy Technology Data Exchange (ETDEWEB) Khemiri, N., E-mail: naoufel_khemiri@yahoo.f [Laboratoire de Photovoltaique et Materiaux Semiconducteurs-ENIT, Universite Tunis, ElManar BP 37, Le belvedere, 1002 Tunis (Tunisia); Sinaoui, A.; Kanzari, M. [Laboratoire de Photovoltaique et Materiaux Semiconducteurs-ENIT, Universite Tunis, ElManar BP 37, Le belvedere, 1002 Tunis (Tunisia) 2011-04-15 CuIn{sub 3}S{sub 5} thin films were prepared from powder by thermal evaporation under vacuum (10{sup -6} mbar) onto glass substrates. The glass substrates were heated from 30 to 200 {sup o}C. The films were characterized for their optical properties using optical measurement techniques (transmittance and reflectance). We have determined the energy and nature of the optical transitions of films. The optical constants of the deposited films were determined in the spectral range 300-1800 nm from the analysis of transmission and reflection data. The Swanepoel envelope method was employed on the interference fringes of transmittance patterns for the determination of variation of refractive index with wavelength. Wemple-Di Domenico single oscillator model was applied to determine the optical constants such as oscillator energy E{sub 0} and dispersion energy E{sub d} of the films deposited at different substrate temperatures. The electric free carrier susceptibility and the ratio of the carrier concentration to the effective mass were estimated according to the model of Spitzer and Fan. 12. A Novel Sr2CuInO3S p-type semiconductor photocatalyst for hydrogen production under visible light irradiation Institute of Scientific and Technical Information of China (English) Yushuai Jia; Jingxiu Yang; Dan Zhao; Hongxian Han; Can Li 2014-01-01 A novel Sr2CuInO3S oxysulfide p-type semiconductor photocatalyst has been prepared by solid state reaction method and it exhibits intriguing visible light absorption properties with a bandgap of 2.3 eV. The p-type semiconductor character of the synthesized Sr2CuInO3S was confirmed by Hall efficient measurement and Mott-Schottky plot analysis. First-principles density functional theory calculations (DFT) and electrochem-ical measurements were performed to elucidate the electronic structure and the energy band locations. It was found that the as-synthesized Sr2CuInO3S photocatalyst has appreciate conduction and valence band positions for hydrogen and oxygen evolution, respectively. Photocat-alytic hydrogen production experiments under a visible light irradiation (λ>420 nm) were carried out by loading different metal and metal-like cocatalysts on Sr2CuInO3S and Rh was found to be the best one among the tested ones. 13. Preparation of Cu2Sn3S7 Thin-Film Using a Three-Step Bake-Sulfurization-Sintering Process and Film Characterization Directory of Open Access Journals (Sweden) Tai-Hsiang Lui 2015-01-01 Full Text Available Cu2Sn3S7 (CTS can be used as the light absorbing layer for thin-film solar cells due to its good optical properties. In this research, the powder, baking, sulfur, and sintering (PBSS process was used instead of vacuum sputtering or electrochemical preparation to form CTS. During sintering, Cu and Sn powders mixed in stoichiometric ratio were coated to form the thin-film precursor. It was sulfurized in a sulfur atmosphere to form CTS. The CTS film metallurgy mechanism was investigated. After sintering at 500°C, the thin film formed the Cu2Sn3S7 phase and no impurity phase, improving its energy band gap. The interface of CTS film is continuous and the formation of intermetallic compound layer can increase the carrier concentration and mobility. Therefore, PBSS process prepared CTS can potentially be used as a solar cell absorption layer. 14. The crystal structure of Gabrielite, Tl2AgCu2As3S7, a new species of Thallium Sulfosalt from Lengenbach, Switzerland DEFF Research Database (Denmark) Balic-Zunic, Tonci; Makovicky, Emil; Karanovic, Ljiljana 2006-01-01 Gabrielite, Tl2AgCu2As3S7, is a new species of sulfosalt mineral occurring in the famous Lengenbach locality, at Binntal, Canton Valais, Switzerland. It was discovered in association with numerous other As sulfosalts, such as hutchinsonite, hatchite, edenharterite, trechmannite, tennantite...... formula Tl6(Ag,Cu)3IV(Cu,Ag)6III[(As,Sb)S3]3[(As,Sb)2S4]3; the empirical formula obtained from the microprobe data is Tl5.95(Ag2.56Cu6.46) 9.02(As8.23Sb0.48) 8.71S21. The name of the mineral honors Walter Gabriel, of Basel, Switzerland, well-known mineral photographer and expert of Lengenbach minerals.... 15. Development of a tunable Fabry-Perot etalon-based near-infrared interference spectrometer for measurement of the HeI 2{sup 3}S-2{sup 3}P spectral line shape in magnetically confined torus plasmas Energy Technology Data Exchange (ETDEWEB) Ogane, S.; Shikama, T., E-mail: shikama@me.kyoto-u.ac.jp; Hasuo, M. [Department of Mechanical Engineering and Science, Graduate School of Engineering, Kyoto University, Kyoto 615-8540 (Japan); Zushi, H. [Research Institute for Applied Mechanics, Kyushu University, Fukuoka 816-8580 (Japan) 2015-10-15 In magnetically confined torus plasmas, the local emission intensity, temperature, and flow velocity of atoms in the inboard and outboard scrape-off layers can be separately measured by a passive emission spectroscopy assisted by observation of the Zeeman splitting in their spectral line shape. To utilize this technique, a near-infrared interference spectrometer optimized for the observation of the helium 2{sup 3}S–2{sup 3}P transition spectral line (wavelength 1083 nm) has been developed. The applicability of the technique to actual torus devices is elucidated by calculating the spectral line shapes expected to be observed in LHD and QUEST (Q-shu University Experiment with Steady State Spherical Tokamak). In addition, the Zeeman effect on the spectral line shape is measured using a glow-discharge tube installed in a superconducting magnet. 16. A facile synthesis of MInSe2 (M = Cu, Ag) via low temperature pyrolysis of single source molecular precursors, [(R3P)2MIn(SeCOAr)4 Indian Academy of Sciences (India) Shamik Ghoshal; Liladhar B Kumbhare; Vimal K Jain; Gautam K Dey 2007-04-01 The reaction of KSeCOAr with InCl3 and [MCl(PR3)2] in benzene afforded bimetallic complexes, [(R3P)2MIn(SeCOAr)4] (PR3 = PEt3 or PPh3; M = Cu or Ag; Ar = –C6H5 (phenyl) or 4-MeC6H4 (tolyl)). The triethylphosphine complexes decomposed rapidly when M = Ag while slowly when M = Cu. All these complexes were characterized by elemental analysis, IR, UV-VIS, NMR (1H, 31P) spectral data. Pyrolysis in a furnace at 300°C gave tetragonal MInSe2 (M = Cu, Ag) structure. Solvothermal decomposition of [(PPh3)2CuIn(SeCOAr)4] in boiling ethylene glycol gave nanorods of CuInSe2 which were characterized by XRD, EDAX, SEM and TEM. 17. Calculation of the total electron excitation cross section in the Born approximation using Slater wave functions for the Li (2s yields 2p), Li (2s yields 3p), Na (3s yields 4p), Mg (3p yields 4s), Ca (4s yields 4p) and K (4s yields 4p) excitations. M.S. Thesis Science.gov (United States) Simsic, P. L. 1974-01-01 Excitation of neutral atoms by inelastic scattering of incident electrons in gaseous nebulae were investigated using Slater Wave functions to describe the initial and final states of the atom. Total cross sections using the Born Approximation are calculated for: Li(2s yields 2p), Na(3s yields 4p), k(4s yields 4p). The intensity of emitted radiation from gaseous nebulae is also calculated, and Maxwell distribution is employed to average the kinetic energy of electrons. 18. Effect of CuO on Crystal Grain Size and Crystal Structure of C3S%CuO对C3S亚晶尺寸及晶型的影响 Institute of Scientific and Technical Information of China (English) 石立安; 柯凯 2012-01-01 Polymorphism of tricalcium silicate were prepared using Ca( OH)2 and SiO2 as crude materials and CuO as doping agent by calcination method. The influences of CuO agent on tricalcium silicate phase polymorphism transformation proeess and grains growth were studied wilh phase diagram analysis, XRD, IR and MDI jade 5. 0 sofeware. The results showed that; according to phase analysis of CaO-CuO and CuO-SiO2, CuO accelerated solid reaction of CaO-SiO2; when added CuO addition, the crystal form and partieles growth were both effected. When CuO addition was below 1. 5% , the reducing tendency of C3S grain growth was prominent; if the addtion exceed 1.5%, the reducing tendency become slow, and crystal form changed from T to R when adding,but when the addition exceed 2.0% , R form change hack to M1. From IR analysis, the symmetrical eharateristic of C3S crystal increased through observation of the spectral at 818 cm-1 and 999 cm-1 fade and appear, and the advection increment and decrease of dilation-libration at 497 cm-1 .%以Ca(OH)2、SiO2化学纯为原料,分别掺人不同质量分数的CuO制备不同晶型组成的C3S,运用相图分析、XRD、IR测试方法及MDI jade5.0软件对C3S亚晶生长、晶型转变过程进行研究.结果表明:由CaO-CuO、CuO-SiO2二元相图分析,CuO促进CaO-SiO2固相反应效应明显；C3S经CuO掺杂后,其晶型转变和亚晶成长均受到影响.当CuO掺量小于1.5％时,C3S亚晶尺寸减小趋势明显,大于1.5％时,减小趋势减缓.且C3S晶型随掺量变化先由T型向R型转换,但掺量大于2.0％时,R型又逐步转换为M1型.同时IR分析表明,818cm-1、999cm-1谱带处的先减弱后增强及497 cm-1伸缩振动平移先增大后减小,均说明C3S晶体对称性先增大后减小的变化趋势. 19. Oxygen atom transfer reactions from dioxygen to phosphines via a bridging sulfur dioxide in a trinuclear cluster complex of rhenium, [(Ph(3)P)(2)N][Re(3)(mu(3)-S)(mu-S)(2)(mu-SO(2))Cl(6)(PMe(2)Ph)(3)]. Science.gov (United States) Saito, Taro; Sunaga, Tomoaki; Sakai, Nobuaki; Nakamura, Yoichi; Yamamoto, Saori; Iriuchijima, Daisuke; Yoza, Kenji 2005-06-13 A trinuclear rhenium sulfide cluster complex, [(Ph(3)P)(2)N][Re(3)(mu(3)-S)(mu-S)(3)Cl(6)(PMe(2)Ph)(3)], synthesized from Re(3)S(7)Cl(7), dimethylphenylphosphine, and [(Ph(3)P)(2)N]Cl is readily converted to a bridging SO(2) complex, [(Ph(3)P)(2)N][Re(3)(mu(3)-S)(mu-S)(2)(mu-SO(2))Cl(6)(PMe(2)Ph)(3)], by reaction with O(2). The oxygen atoms on the SO(2) ligand react with phosphines or phosphites to form phosphine oxides or phosphates, and the original cluster complex is recovered. The reaction course has been monitored by (31)P NMR as well as by UV-vis spectroscopy. The catalytic oxygenation of PMePh(2) in the presence of the SO(2) complex shows that turnovers are 8 per hour at 23 degrees C in CDCl(3). The X-ray structures of the cluster complexes are described. 20. Syntheses, crystal Structures and electronic Structures of new metal chalcoiodides Bi{sub 2}CuSe{sub 3}I and Bi{sub 6}Cu{sub 3}S{sub 10}I Energy Technology Data Exchange (ETDEWEB) Liang, I-Chu [Department of Chemistry, and Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan (China); Bilc, Daniel I. [Department of Molecular & Biomolecular Physics, National Institute for Research & Development of Isotopic & Molecular Technologies, Cluj-Napoca 400293 (Romania); Manoli, Maria [Department of Mechanical and Manufacturing Engineering, University of Cyprus, 1678 Nicosia (Cyprus); Chang, Wei-Yun; Lin, Wen-Fu [Department of Chemistry, and Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan (China); Kyratsi, Theodora [Department of Mechanical and Manufacturing Engineering, University of Cyprus, 1678 Nicosia (Cyprus); Hsu, Kuei-Fang [Department of Chemistry, and Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan (China) 2016-02-15 Two new metal chalcoiodides were synthesized by solid-state reactions at 400 °C. Crystal Data: Bi{sub 2}CuSe{sub 3}I, 1, monoclinic, C2/m, a=14.243(2) Å, b=4.1937(7) Å, c=14.647(2) Å, β=116.095(2)°, V=785.7(2) Å{sup 3}, and Z=4; Bi{sub 6}Cu{sub 3}S{sub 10}I, 2, orthorhombic, Pnma, a=17.476(2) Å, b=4.0078(4) Å, c=27.391(2) Å, V=1918.5(3) Å{sup 3}, and Z=4. Compound 1 adopts a three-dimensional structure formed by two alternative layers, which consist of BiSe{sub 5} square pyramids, BiSe{sub 4}I{sub 2} octahedra, CuSe{sub 4} tetrahedra, and CuSe{sub 2}I{sub 2} tetrahedra. Compound 2 possesses a new open framework built up of BiS{sub 5} square pyramides, BiS{sub 6} octahedra, BiS{sub 8} polyhedra, and CuS{sub 4} tetrahedra where I{sup −} anions are uniquely trapped within the tunnels. Both electronic structures reveal that bismuth and chalcogenide orbitals dominate the bandgaps. The Cu d and I p states contribute to the top of valence bands, in which the distribution of I orbitals may correspond to the relative bonding interactions in 1 and 2. The optical bandgaps determined by the diffuse reflectance spectra are 0.68 eV and 0.72 eV for 1 and 2, respectively. 1 is a p-type semiconductor with high Seebeck coefficients of 460–575 μV/K in the temperature range of 300–425 K. The electrical conductivity is 0.02 S/cm at 425 K for the undoped sample. The thermal conductivity is 0.22 W/mK at 425 K. - Graphical abstract: The hybridization of chalcogenides and iodides produces two new solids Bi2CuSe3I and Bi6Cu3S10I. The I{sup −} anions participate in distinct bonding interactions within the two structures and that is consistent with the analyses of density of states. 1 is a p-type semiconductor with an optical bandgap of 0.68 eV, which possesses high Seebeck coefficient and low lattice thermal conductivity in 300–425 K. 1. 糖精铜水合物[Cu(C7H4NO3S)2(H2O)4]·2H2O的合成及晶体结构%Synthesis and Crystal Structure of Tetraaquabis(saccharinato-N)Copper( Ⅱ ) Dihydrate Compound [Cu(C7H4NO3S)2 (H2O)4]· 2H2O Institute of Scientific and Technical Information of China (English) 曹云丽; 李青彬; 张爽爽 2012-01-01 首次以糖精钠、丙氨酸和硫酸铜为原料合成得到糖精铜蓝色块状晶体,采用红外光谱、X-射线单晶衍射对配合物的晶体结构进行了表征.证明该晶体属单斜晶系,空间群为P21/c,晶胞参数为:a=8,4012(19)A,b=16.3605(18)(A),c=7.3479(16)(A),a=90°,β=100.84(2)°,V=991.49(15)(A)3,Z=2,Dc=1.794 g·cm-3,F(000)=550,R=0.0309.结构分析表明,中心Cu2+与4个O原子和2个N原子配位,处于四角双锥八面体配位环境中,4个水分子在赤道平面上与Cu2+配位,晶胞中还包含有2个游离的结晶水,形成了含6个水分子的结晶物.%Tetraaquabis(saccharinato-N) copper(Ⅱ) dihydrate compound [Cu(C7 H4 NO3 S)2 ( H2O)4] ·2H2O is synthesized by using saccharin sodium, alanine, and copper sulfate as raw materials. And its crystal structure is determined by infrared spectrometry and X-ray diffraction single crystal analysis. The crystal is of monoclinic,space group P21/c,a = 8. 4012(19)A,b=16. 3605(18)A,c=7. 3479(16) A,α = 90°,β=100. 84(2)°,V = 991. 49(15)A3,Z=2,Dc= 1.794 g· cm-3,F(000) = 550,R = 0, 0309. Center Cu2+ is located in the four-cor-ner bipyramid coordination environment with coordination of four atoms 0 and two atoms N. Four molecules H2O coordinate to Cu2+ above the equatorial plane,and the crystal cell contains two free crystal water molecules, so it forms the six-water crystal compound. 2. On the kinetic and thermodynamic fragility of the Pt{sub 60}Cu{sub 16}Co{sub 2}P{sub 22} and Pt{sub 57.3}Cu{sub 14.6}Ni{sub 5.3}P{sub 22.8} bulk metallic glasses Energy Technology Data Exchange (ETDEWEB) Gallino, Isabella, E-mail: i.gallino@mx.uni-saarland.de [Saarland University, Department of Materials Science and Engineering, Campus C6.3, 66123 Saarbruecken (Germany); Gross, Oliver [Saarland University, Department of Materials Science and Engineering, Campus C6.3, 66123 Saarbruecken (Germany); Dalla Fontana, Giulia [Department of Chemistry IFM and NIS, University of Torino, V. Giuria 7, 10125 Torino (Italy); Evenson, Zach; Busch, Ralf [Saarland University, Department of Materials Science and Engineering, Campus C6.3, 66123 Saarbruecken (Germany) 2014-12-05 Highlights: • The studied Pt–Cu–(Ni,Co)–P glasses are more fragile than Zr-based alloys. • They show large increases in the C{sub p} at T{sub g} and small barriers for cooperative rearrangements of atoms. • They have fragility parameters among the lowest reported for BMG systems (D{sup *} = 10–12). • They crystallize into a state that melts with distinctly high entropy of fusion. • The microscopic origin of their fragility seems different than that for Zr- and Pd-based BMGs. - Abstract: The investigations in this study focus on bulk metallic glass (BMG) alloy families based on noble metals like Pt, which are more kinetically fragile than Zr-based BMG systems. Thermophysical properties have been investigated by calorimetry and thermal mechanical analyses for the determination of the specific heat capacity and viscosity, respectively. For the Pt{sub 60}Cu{sub 16}Co{sub 2}P{sub 22} and Pt{sub 57.3}Cu{sub 14.6}Ni{sub 5.3}P{sub 22.8} BMG compositions consistent Vogel–Fulcher–Tammann (VFT) fits of the viscosity measurements are established, and the temperature dependence of the configurational entropy is calculated from thermodynamic data. Fits to the Adam–Gibbs equation are performed using this configurational entropy change. Their fragile nature is compared to that of Zr-based alloys in terms of structural considerations. 3. Interface reactions in Mg/Zn3P2 solar cells Science.gov (United States) Kazmerski, L. L.; Ireland, P. J.; Catalano, A. 1981-05-01 The composition and chemistry of the Mg/Zn3P2 interface was studied using surface analysis techniques. For higher carrier concentrations (p 10 to the 17th power/cu cm), a heterojunction occurs by the chemical interaction of the Mg with Zn3P2 to form Mg3P2. For lower carrier concentrations this reaction does not take place, and Mg diffuses into the Zn3P2. Diffusion coefficients of Mg in Zn3P2 are determined. Grain (crystalline) and grain boundary components of the diffusion coefficient are evaluated. These data compare very well with that reported using electron beam induced currents and spectral response measurements. 4. O(^3 p) Doped Helium Droplets Science.gov (United States) Brice, Joseph T.; Douberly, Gary E. 2017-06-01 Atomic oxygen (^3 P) is generated via thermolysis in a commerical thermal gas cracker (Mantis Ltd. MGC-75). Complexes with HCN were investigated to qualitatively assess the doping efficiency of O(^3 P) into a helium droplet. Theoretical calculations of a linear O \\cdot\\cdot\\cdot HCN (^3 Σ) complex at the CCSD(T)/aug-cc-pVTZ level are consistent with the rotational constants extracted from the rotational substructure in the experimental spectra, and with dipole moments approximated from Stark spectra. The thermal source will be used to study reactions between O(^3 P) and hydrocarbons in helium droplets, and preliminary data on this topic will be presented. 5. Copper(I) arenethiolates with intramolecular coordination and the formation of mixed organo(arenethiolate) copper(I) aggregates. X-ray structures of trimeric [Cu(S-1-C10H6NMe2-8)]9, and hexanuclear [Cu3(S-1-C10H6NMe2-8)2(C_CtBu)]2 NARCIS (Netherlands) Koten, G. van; Janssen, M.D.; Donkervoort, J.G.; Berlekom, S.B. van; Spek, A.L.; Grove, D.M. 1996-01-01 Two new copper(I) arenethiolates with ortho-chelating nitrogen donor atoms [Cu(SC6H4NMe2-2)]3 (5) and [Cu(S-1-C10H6NMe2-8)]9 (6) have been synthesized by reacting Me3SiSAr (Ar = C6H4NMe2-2 or 1-C10H6NMe2-8) with copper(I) chloride in a 1:1 molar ratio. Copper(I) arenethiolate 5 is a white to pale ye 6. Coinage metal complexes supported by a "PN(3)P" scaffold. Science.gov (United States) Rao, Gyandshwar Kumar; Gorelsky, Serge I; Korobkov, Ilia; Richeson, Darrin 2015-11-28 A series of monovalent group 11 complexes, [2,6-{Ph2PNMe}2(NC5H3)]CuBr 1, [2,6-{Ph2PNMe}2(NC5H3)]CuOTf 2, [2,6-{Ph2PNMe}2(NC5H3)]AgOTf 3, and [2,6-{Ph2PNMe}2(NC5H3)](AuCl)24, supported by a neutral PN(3)P ligand have been synthesized and characterized by multinuclear NMR and single crystal X-ray diffraction studies. The variation of the coordination properties were analyzed and electronic structure calculations have been carried out to provide insight on the bonding details in these complexes. The Cu(I) complexes displayed an unusual coordination geometry with a tridentate pincer ligand and an overall four coordinate trigonal pyramidal geometry. In contrast the Ag(I) analogue displayed a bidentate κ(2)-P,P' ligation leaving the pyridyl-N atom uncoordinated and yielding a pyramidalized trigonal planar geometry around Ag. The bimetallic Au(I) complex completed the series and displayed a monodentate P-bonded ligand and a linear coordination geometry. 7. Thermoelastic properties of Zn3P2 DEFF Research Database (Denmark) Gerward, Leif; Olsen, J. Staun; Waśkowska, A. 2011-01-01 The bulk modulus and thermal expansion of Zn3P2 has been investigated at pressures up to 21GPa and temperatures down to 100K. The experimental zero-pressure bulk modulus is 80.7 ± 1.8GPa, in accordance with the bulk modulus scaling and lattice properties of the related compound Cd3P2. A tetragonal...... to orthorhombic phase transformation occurs above 11GPa with a relative volume change of-7.1%. Values for the thermal expansion coefficient are reported at 293, 200 and 100K.... 8. Atomic scattering in the diffraction limit: electron transfer in keV Li+-Na(3s, 3p) collisions DEFF Research Database (Denmark) Poel, Mike van der; Nielsen, C.V.; Rybaltover, M. 2002-01-01 We measure angle differential cross sections (DCS) in Li+ + Na --> Li + Na+ electron transfer collisions in the 2.7-24 keV energy range. We do this with a newly constructed apparatus which combines the experimental technique of cold target recoil ion momentum spectroscopy with a laser-cooled target... 9. Electrical Conduction of Some Fe3S3 Ring Compounds Institute of Scientific and Technical Information of China (English) WANG Jian; LIU Ping; YOU Gang 2003-01-01 @@ M3S3 cluster six-membered ring compounds are very interesting compounds liking aromatic benzene, and Cu, Pd, B and so on can also form M3S3 cluster besides Fe. [1,2] In recent years electrical conduction of some metal com plexes has been studied. [3,4] The study of electrical conduction through DNA molecules has illustrated that DNA is a semiconductor, but its principle of electrical conduction has not been revealed. [5,6] Electrical conduction of artificial synthesized Fe-S cluster compounds has not been reported. We hope to infer the function on electrical conduction of metal active centers of iron-sulfur proteins through study of electrical conduction of Fe3S3 ring compounds, paving the way for the discovery of new advanced biological functional materials. 10.$^3P_2$Superfluids Are Topological CERN Document Server Mizushima, Takeshi 2016-01-01 We clarify the topology of the$^3P_2$superfluidity which is expected to be realized in the cores of neutron stars and cubic odd-parity superconductors. The phase diagram includes the unitary uniaxial/biaxial nematic phases and nonunitary ferromagnetic and cyclic phases. We here show that the low-energy structures of all the phases are governed by different types of topologically protected gapless fermionic excitations: Surface Majorana fermions in nematic phases, single itinerant Majorana fermion in the ferromagnetic phase, and a quartet of itinerant Majorana fermions in the cyclic phase. Using the superfluid Fermi liquid theory, we also demonstrate that dihedral-two and -four biaxial nematic phases are thermodynamically favored in the weak coupling limit under a magnetic field. The mass acquisition of surface Majorana fermions in nematic phases is subject to symmetry. 11. BOREAS Level-3p Landsat TM Imagery: Geocoded and Scaled At-sensor Radiance Science.gov (United States) Nickeson, Jaime; Knapp, David; Newcomer, Jeffrey A.; Hall, Forrest G. (Editor); Cihlar, Josef 2000-01-01 For BOReal Ecosystem-Atmosphere Study (BOREAS), the level-3p Landsat Thematic Mapper (TM) data were used to supplement the level-3s Landsat TM products. Along with the other remotely sensed images, the Landsat TM images were collected in order to provide spatially extensive information over the primary study areas. This information includes radiant energy, detailed land cover, and biophysical parameter maps such as Fraction of Photosynthetically Active Radiation (FPAR) and Leaf Area Index (LAI). Although very similar to the level-3s Landsat TM products, the level-3p images were processed with ground control information, which improved the accuracy of the geographic coordinates provided. Geographically, the level-3p images cover the BOREAS Northern Study Area (NSA) and Southern Study Area (SSA). Temporally, the four images cover the period of 20-Aug-1988 to 07-Jun-1994. Except for the 07-Jun-1994 image, which contains seven bands, the other three contain only three bands. 12. Spectra and oscillator strengths of 3p63d9-3p53d10 and 3p63d9-3p63d84p transitions for cobalt-like Sn23+ ion Institute of Scientific and Technical Information of China (English) Chen Ming-Lun; Yu Xiao-Guang 2009-01-01 This paper calculates the spectra and oscillator strengths for highly ionized cobalt-like Sn23+ ions 3p63d9-3p53d10,3p63d9 - 3p63d84p transitions by using a multi-configuration self-consistent field method program together with the proposed fitting formula. The calculations have a good agreement with observations. 13. Photoemission time-delay measurements and calculations close to the 3s ionization minimum in Ar CERN Document Server Guenot, D; Arnold, C L; Kroon, D; Dahlstrom, J M; Miranda, M; Fordell, T; Gisselbrecht, M; Johnsson, P; Mauritsson, J; Lindroth, E; Maquet, A; Taieb, R; L'Huillier, A; Kheifets, A S 2012-01-01 We present experimental measurements and theoretical calculations of photoionization time delays from the$3s$and$3pshells in Ar in the photon energy range of 32-42 eV. The experimental measurements are performed by interferometry using attosecond pulse trains and the infrared laser used for their generation. The theoretical approach includes intershell correlation effects between the 3s and 3p shells within the framework of the random phase approximation with exchange (RPAE). The connection between single-photon ionization and the two-color two-photon ionization process used in the measurement is established using the recently developed asymptotic approximation for the complex transition amplitudes of laser-assisted photoionization. We compare and discuss the theoretical and experimental results especially in the region where strong intershell correlations in the 3s to kp channel lead to an induced "Cooper" minimum in the 3s ionization cross-section. 14. Determination of diffusion, reflection and deexcitation coefficients of metastable excited Ne(3P2) atom Science.gov (United States) Suzuki, S.; Itoh, H. 2016-05-01 The diffusion coefficient of the metastable excited Ne(3P2) atom in neon, the reflection coefficient of Ne(3P2) at the surface of an electrode and the rate coefficient of Ne(3P2) for collisional quenching by Ne(1S0) were determined from the gas pressure dependence of the effective lifetime of Ne(3P2). The effective lifetime of Ne(3P2) was measured from the transient current after turning off the Ultraviolet (UV) light in a Townsend discharge. The observed transient current waveform was analysed by solving the diffusion equation for the metastable excited Ne(3P2) atom using the third kind of boundary condition. The rate coefficient of Ne(3P2) for collisional quenching by Ne(1S0) and the reflection coefficient were determined by a nonspectroscopic method for the first time in neon to the best of our knowledge and were (3.2 ± 0.4) × 10-16 cm3 s-1 and 0.10 ± 0.04, respectively. The obtained diffusion coefficient at 1 Torr was 177 ± 17 cm2 s-1, which is consistent with the value reported by Dixon and Grant. Moreover, the present results are compared with the results of Phelps and were found to be in good agreement. We also discuss the deexcitation rate of Ne(3P2) at pressures of up to 60 Torr in comparison with previously reported values. 15. Assessment of combined toxicity of heavy metals from industrial wastewaters on Photobacterium phosphoreum T3S Science.gov (United States) Zeb, BibiSaima; Ping, Zheng; Mahmood, Qaisar; Lin, Qiu; Pervez, Arshid; Irshad, Muhammad; Bilal, Muhammad; Bhatti, Zulfiqar Ahmad; Shaheen, Shahida 2017-07-01 This research work is focusing on the toxicities of heavy metals of industrial origin to anaerobic digestion of the industrial wastewater. Photobacterium phosphoreum T3S was used as an indicator organism. The acute toxicities of heavy metals on P. phosphoreum T3S were assessed during 15-min half inhibitory concentration (IC50) as indicator at pH 5.5-6. Toxicity assays involved the assessment of multicomponent mixtures using TU and MTI approaches. The results of individual toxicity indicated that the toxicity of Cd, Cu and Pb on P. phosphoreum increased with increasing concentrations and there was a linear correlation. The 15-min IC50 values of Cd, Cu and Pb were 0.537, 1.905 and 1.231 mg/L, respectively, and their toxic order was Cd > Pb > Cu. The combined effects of Cd, Cu and Pb were assayed by equivalent concentration mixing method. The results showed that the combined effects of Cd + Cu, Cd + Pb, Cu + Pb, Cd + Cu + Pb were antagonistic, antagonistic and partly additive. The combined effect of three heavy metals was partly additive. 16. Measurement of Integrated Stokes Parameters for He 3 3p State Excited by Spin-Polarized Electrons Institute of Scientific and Technical Information of China (English) DING Hai-Bing; PANG Wen-Ning; LIU Yi-Bao; SHANG Ren-Cheng 2005-01-01 @@ Integrated Stokes parameters Pi (i = 1, 2, 3) for the He 3 3p → 2 3S1 (388.9nm) transition after excitation from the ground state to the 3 3 P state by a transversely spin-polarized electron beam are measured in near threshold energy region. The experimental results are presented. The linear-polarization P2 are consistent with zero over the incident energy range, providing evidence for the LS coupling mechanism of the 3 3P state. The measured circular polarization P3 are non-zero, indicating strong electron-electron exchange effects in the spin-polarized electron-atom collision process. 17. Orbital alignment dependence of electron transfer cross sections. I : H+-Na(3p) near the matching velocity NARCIS (Netherlands) Müller, U.; Meijer, H.A.J.; Holme, N.C.R.; Kmit, M.; Lauritsen, J.H.V.; Pedersen, J.O.P.; Richter, C.; Thomsen, J.W.; Andersen, N.; Nielsen, S.E. 1995-01-01 This paper reports experimental and theoretical results for the influence of target excitation and orbital alignment on the charge exchange process for the system H+-Na(3s,3p). The experimental velocity range covers for the first time the region around and beyond the "matching velocity" of 0.47 a.u. 18. Mechanism and kinetics for the reaction of O(3P) with DMSO: A theoretical study Science.gov (United States) Mandal, Debasish; Bagchi, Sabyasachi; Das, Abhijit K. 2012-11-01 Mechanism and kinetics for the reaction of DMSO with O(3P) have been investigated by M06-2X/MG3S, CBS-QB3 and G4MP2 methods. Four possible reaction pathways are identified. Among them, the O(3P) addition to S-atom followed by CH3 elimination is almost exclusive. Four pre-reactive complexes have been located. AIM theory is used to determine the nature of interactions in these complexes. Considering the formation of pre-reactive complex, the rate constant for major pathway is calculated using transition state theory applied to a two-step mechanism. Enthalpies of formation at 298.15 K (ΔfH°298.15) have been calculated using the composite CBS-QB3, G4MP2 and G3B3 methods. 19. Safety assessment of [3S, 3'S]-astaxanthin--Subchronic toxicity study in rats. Science.gov (United States) Buesen, R; Schulte, S; Strauss, V; Treumann, S; Becker, M; Gröters, S; Carvalho, S; van Ravenzwaay, B 2015-07-01 Astaxanthin, a naturally occurring xanthophyll, is commercially used as a coloring agent in salmon feed, but also marketed as a dietary supplement. The objective of this study was to investigate the subchronic toxicity of synthetic [3S, 3'S]-Astaxanthin in rats. A powder formulation containing approximately 20% [3S, 3'S]-Astaxanthin was administered via the diet to groups of 10 male and 10 female Wistar rats at concentrations of 5000, 15,000 and 50,000 ppm for a period of 13 weeks. A formulation of comparable composition but without [3S, 3'S]-Astaxanthin served as a placebo control. There were no effects observed on survival, clinical examinations, clinical pathology, estrous cycle as well as on sperm parameters. At terminal necropsy, a macroscopically visible brown-blue discoloration of the gastrointestinal contents was noted which was considered to be secondary to the violet-brown color of the test material. No other significant or dose-related abnormalities were found in the tissues collected at termination. Our observations support that ingestion of [3S, 3'S]-Astaxanthin of up to 700-920 mg/kg bw/day in rats in a gelatin/carbohydrate formulation is without adverse effects. 20. HECT E3s and human disease Directory of Open Access Journals (Sweden) Staub Olivier 2007-11-01 Full Text Available Abstract In a simplified view, members of the HECT E3 family have a modular structure consisting of the C-terminal HECT domain, which is catalytically involved in the attachment of ubiquitin to substrate proteins, and N-terminal extensions of variable length and sequence that mediate the substrate specificity of the respective HECT E3. Although the physiologically relevant substrates of most HECT E3s have remained elusive, it is becoming increasingly clear that HECT E3s play an important role in sporadic and hereditary human diseases including cancer, cardiovascular (Liddle's syndrome and neurological (Angelman syndrome disorders, and/or in disease-relevant processes including bone homeostasis, immune response and retroviral budding. Thus, molecular approaches to target the activity of distinct HECT E3s, regulators thereof, and/or of HECT E3 substrates could prove valuable in the treatment of the respective diseases. Publication history: Republished from Current BioData's Targeted Proteins database (TPdb; http://www.targetedproteinsdb.com. 1. Where are\\chi_{cJ}(3P)$? CERN Document Server Chen, Dian-Yong 2016-01-01 In the present work, we propose the$Y(4140)$as the$\\chi_{c1}(3P)$state by studying the$\\chi_{c1} \\pi^+ \\pi^-$invariant mass spectrum of the$B\\to K \\chi_{c1} \\pi^+ \\pi^-$process. In the$D\\bar{D}$invariant mass spectrum of the$B\\to K D\\bar{D}$process, we find a new resonance with the mass and width to be$ (4083.0 \\pm 5.0) $and$ (24.1 \\pm 15.4) $MeV, respectively, which could be a good candidate of the$\\chi_{c0}(3P)$state. The theoretical investigations on the decay behaviors of the$\\chi_{cJ}(3P)$in the present work support the assignments of the$Y(4140)$and$Y(4080)$as the$\\chi_{c1}(3P)$and$\\chi_{c0}(3P)$states, respectively. In addition, the$\\chi_{c2}(3P)state is predicted to be a very narrow state. The results in the present work could be tested by further experiments in the LHCb and forthcoming Belle II. 2. On the isotope effect in compressed superconducting H3S and D3S Science.gov (United States) Harshman, Dale R.; Fiory, Anthony T. 2017-04-01 A maximum superconductive transition temperature T C = 203.5 K has recently been reported for a sample of the binary compound tri-hydrogen sulfide (H3S) prepared at high pressure and with room temperature annealing. Measurements of T C for H3S and its deuterium counterpart D3S have suggested a mass isotope effect exponent α with anomalous enhancements for reduced applied pressures. While widely cited for evidence of phonon-based superconductivity, the measured T C is shown to exhibit important dependences on the quality and character of the H3S and D3S materials under study; examination of resistance versus temperature data shows that variations in T C and apparent α are strongly correlated with residual resistance ratio, indicative of sensitivity to metallic order. Correlations also extend to the fractional widths of the superconducting transitions. Using resistance data to quantify and compensate for the evident materials differences between H3S and D3S samples, a value of α = 0.043 ± 0.140 is obtained. Thus, when corrected for the varying levels of disorder, the experimental upper limit (≤0.183) lies well below α derived in phonon-based theories. 3. A developmental toxicity study of 3S, 3'S-Astaxanthin in New Zealand white rabbits. Science.gov (United States) Schneider, Steffen; Mellert, Werner; Schulte, Stefan; van Ravenzwaay, Bennard 2016-04-01 Astaxanthin, a naturally occurring pigment used to give the characteristic orange-pink colour to salmonid fish reared in aquaculture, is also marketed as a dietary supplement. Synthetic 3S, 3'S-Astaxanthin was tested for potential harmful effects on the in utero development of New Zealand white rabbits in a study according to international regulatory guidelines. There were two control groups, one being a placebo administration and three dose levels corresponding to 100, 200, and 400 mg of 3S, 3'S-Astaxanthin per kg body weight/day. The group sizes varied from 23 to 27 litters, providing approximately 200 fetuses per group for evaluation of developmental toxicity. There were no significant effects on the health of the does, nor on the size and viability of the litters. Malformations, both external and internal, were rare and occurred in all groups, including controls with no indication of a treatment relationship. Variations were much more common, being found in all litters. However, when examined by type and frequency, no pattern emerged indicating a relationship to administration of the test substance. It is concluded that administration of 3S, 3'S-Astaxanthin in a gelatin/carbohydrate powder formulation throughout pregnancy up to 400 mg/kg body weight/day is without harmful effects on reproduction or fetal development. 4. Ionic rotational branching ratios in resonant enhanced multiphoton ionization of NO via the A2Sigma(+)(3s sigma) and D2Sigma(+)(3p sigma) states Science.gov (United States) Rudolph, H.; McKoy, V.; Dixit, S. N.; Huo, W. M. 1988-01-01 Results are presented for the rotationally resolved photoelectron spectra resulting from a (2 + 1) one-color resonant enhanced multiphoton ionization (REMPI) of NO via the rotationally clean S21(11.5) and mixed S11(15.5) + R21(15.5) branches of the 0-0 transition in the D-X band. The calculations were done in the fixed-nuclei frozen core approximation. The resulting photoionization spectra, convoluted with a Lorentzian detection function, agree qualitatively with experimental results of Viswanathan et al. (1986) and support their conclusion that the nonspherical nature of the molecular potential creates a substantial l-mixing in the continuum, which in turn leads to the intense Delta N = 0 peak. The rather strong photoelectron energy dependence of the rotational branching ratios of the D 2Sigma(+) S21(11.5) line was investigated and compared to the weak energy dependence of the A 2Sigma(+) R22(21.5) line. 5. Some recent applications of XTRAN3S Science.gov (United States) Seidel, D. A.; Bennett, R. M.; Ricketts, R. H. 1983-01-01 A time marching finite difference code, XTRAN3S that solves the three dimensional transonic small perturbation equation for flow over isolated wings was developed. During initial applications of the program, problems were encountered in the prediction of unsteady forces. The use of a revised grid and force calculation scheme improved those predictions. Comparisons are made between predicted and experimental pressure data for a rectangular supercritical wing. Comparisons of steady and unsteady data at M sub infinity = 0.700 show good agreement between calculated and experimental values. A comparison of steady data at M sub infinity 0.825 shows poor agreement between calculations and experiment. Program difficulties were encountered with swept and tapered configurations. 6. Structural, electronic, and elastic properties of CuFeS2: first-principles study Science.gov (United States) Zhou, Meng; Gao, Xiang; Cheng, Yan; Chen, Xiangrong; Cai, Lingcang 2015-03-01 The structural, electronic, and elastic properties of CuFeS2 have been investigated by using the generalized gradient approximation (GGA), GGA + U (on-site Coulomb repulsion energy), the local density approximation (LDA), and the LDA + U approach in the frame of density functional theory. It is shown that when the GGA + U formalism is selected with a U value of 3 eV for the 3d state of Fe, the calculated lattice constants agree well with the available experimental and other theoretical data. Our GGA + U calculations indicate that CuFeS2 is a semiconductor with a band gap of 0.552 eV and with a magnetic moment of 3.64 µB per Fe atom, which are well consistent with the experimental results. Combined with the density of states, the band structure characteristics of CuFeS2 have been analyzed and their origins have been specified, which reveals a hybridization existing between Fe-3d, Cu-3s, and S-3p, respectively. The charge and Mulliken population analyses indicate that CuFeS2 is a covalent crystal. Moreover, the calculated elastic constants prove that CuFeS2 is mechanically stable but anisotropic. The bulk modulus obtained from elastic constants is 87.1 GPa, which agrees well with the experimental value of 91 ± 15 GPa and better than the theoretical bulk modulus 74 GPa obtained from GGA method by Lazewski et al. The obtained shear modulus and Debye temperature are 21.0 GPa and 287 K, respectively, and the latter accords well with the available experimental value. It is expected that our work can provide useful information to further investigate CuFeS2 from both the experimental and theoretical sides. 7. Superplasticity in an Aluminum Alloy 6061/A12O3p Composite Institute of Scientific and Technical Information of China (English) 2001-01-01 The superplasticity of an Al2O3p/6061Al composite, fabricated by powder metallurgy techniques,has been investigated. Instead of any special thermomechanical processing or hot rolling, simple hot extrusion has been employed to obtain a fine grained structure before superplastic testing.Superplastic tensile tests were performed at strain rates ranging from 10-2 to 10-4 s-1 and at temperatures from 833 to 893 K. A maximum elongation of 200% was achieved at a temperature of 853 K and an initial strain rate of 1.67×10-3 s-1. The highest value obtained for the strain rate sensitivity index (m) was 0.32. Differential scanning calorimeter was used to ascertain the possibility of any partial melting in the vicinity of optimum superplastic temperature. These results suggested that no liquid phase existed where maximum elongation was achieved and deformation took place entirely in the solid state. 8. Gluon fragmentation into {sup 3} P{sub J} quarkonium Energy Technology Data Exchange (ETDEWEB) Ma, J.P. 1995-10-01 The functions of the gluon fragmentation into {sup 3}P{sub j} quarkonium are calculated to order {alpha}{sup 2}{sub s}. With the recent progress in analysing quarkonium systems in QCD it is possible show how the so called divergence in the limit of the zero-binding energy, which is related to P-wave quarkonia, is treated correctly in the case of fragmentation functions. The obtained fragmentation functions satisfy explicitly at the order of {alpha} {sup 2}{sub s} the Altarelli-Parisi equation and when z {yields} 0 they behave as z{sup -1} as expected. 19 refs., 7 figs. 9. Products of the Benzene + O(3P) Reaction Energy Technology Data Exchange (ETDEWEB) Taatjes, Craig A.; Osborn, David L.; Selby, Talitha M.; Meloni, Giovanni; Trevitt, Adam J.; Epifanovsky, Evgeny; Krylov, Anna I.; Sirjean, Baptiste; Dames, Enoch; Wang, Hai 2009-12-21 The gas-phase reaction of benzene with O(3P) is of considerable interest for modeling of aromatic oxidation, and also because there exist fundamental questions concerning the prominence of intersystem crossing in the reaction. While its overall rate constant has been studied extensively, there are still significant uncertainties in the product distribution. The reaction proceeds mainly through the addition of the O atom to benzene, forming an initial triplet diradical adduct, which can either dissociate to form the phenoxy radical and H atom, or undergo intersystem crossing onto a singlet surface, followed by a multiplicity of internal isomerizations, leading to several possible reaction products. In this work, we examined the product branching ratios of the reaction between benzene and O(3P) over the temperature range of 300 to 1000 K and pressure range of 1 to 10 Torr. The reactions were initiated by pulsed-laser photolysis of NO2 in the presence of benzene and helium buffer in a slow-flow reactor, and reaction products were identified by using the multiplexed chemical kinetics photoionization mass spectrometer operating at the Advanced Light Source (ALS) of Lawrence Berkeley National Laboratory. Phenol and phenoxy radical were detected and quantified. Cyclopentadiene and cyclopentadienyl radical were directly identified for the first time. Finally, ab initio calculations and master equation/RRKM modeling were used to reproduce the experimental branching ratios, yielding pressure-dependent rate expressions for the reaction channels, including phenoxy + H, phenol, cyclopentadiene + CO, which are proposed for kinetic modeling of benzene oxidation. 10. Thermoelectric properties of polycrystalline NiSi3P4 Science.gov (United States) May, Andrew F.; McGuire, Michael A.; Wang, Hsin 2013-03-01 The Hall and Seebeck coefficients, electrical resistivity, and thermal conductivity of polycrystalline NiSi3P4 were characterized from 2 to 775 K. Undoped NiSi3P4 behaves like a narrow gap semiconductor, with activated electrical resistivity ρ below room temperature and a large Seebeck coefficient of ˜400 μV/K at 300 K. Attempts to substitute boron for silicon resulted in the production of extrinsic holes, yielding moderately doped semiconductor behavior with ρ increasing with increasing temperature above ˜150 K. Hall carrier densities are limited to approximately 5 × 1019 cm-3 at 200 K, which would suggest the solubility limit of boron is reached if boron is indeed incorporated into the lattice. These extrinsic samples have a Hall mobility of ˜12 cm2/V/s at 300 K, and a parabolic band equivalent effective mass of ˜3.5 times the free electron mass. At 700 K, the thermoelectric figure of merit zT reaches ˜0.1. Further improvements in thermoelectric performance would require reaching higher carrier densities, as well as a mechanism to further reduce the lattice thermal conductivity, which is ˜5 W/m/K at 700 K. Alloying in Ge results in a slight reduction of the thermal conductivity at low temperatures, with little influence observed at higher temperatures. 11. Microstructure and Strength of Brazed Joints of Ti3Al Base Alloy with Cu-P Filler Metal Institute of Scientific and Technical Information of China (English) Peng HE; Jicai FENG; Heng ZHOU 2005-01-01 Brazing of Ti3Al alloys with the filler metal Cu-P was carried out at 1173～1273 K for 60～1800 s. When products are brazed, the optimum brazing parameters are as follows: brazing temperature is 1215～1225 K; brazing time is 250～300 s. Four kinds of reaction products were observed during the brazing of Ti3Al alloys with the filler metal Cu-P, i.e., Ti3Al phase with a small quantity of Cu (Ti3Al(Cu)) formed close to the Ti3Al alloy; the TiCu intermetallic compounds layer and the Cu3P intermetallic compounds layer formed between Ti3Al(Cu) and the filler metal, and a Cu-base solid solution formed with the dispersed Cu3P in the middle of the joint. The interfacial structure of brazed Ti3Al alloys joints with the filler metal Cu-P is Ti3Al/Ti3Al(Cu)/TiCu/Cu3P/Cu solid solution (Cu3P)/Cu3P/TiCu/Ti3Al(Cu)/Ti3Al, and this structure will not change with brazing time once it forms. The thickness of TiCu+Cu3P intermetallic compounds increases with brazing time according to a parabolic law. The activation energy Q and the growth velocity K0 of reaction layer TiCu+Cu3P in the brazed joints of Ti3Al alloys with the filler metal Cu-P are 286 k J/mol and 0.0821 m2/s, respectively, and growth formula was y2=0.0821exp(-34421.59/T)t.Careful control of the growth for the reaction layer TiCu+Cu3P can influence the final joint strength. The formation of the intermetallic compounds TiCu+Cu3P results in embrittlement of the joint and poor joint properties. The Cu-P filler metal is not fit for obtaining a high-quality joint of Ti3Al brazed. 12. Kinetics of the multichannel reaction of methanethiyl radical (CH3S*) with 3O2. Science.gov (United States) Zhu, Li; Bozzelli, Joseph W 2006-06-01 The CH3S* + O2 reaction system is considered an important process in atmospheric chemistry and in combustion as a pathway for the exothermic conversion of methane-thiyl radical, CH3S*. Several density functional and ab initio computational methods are used in this study to determine thermochemical parameters, reaction paths, and kinetic barriers in the CH3S* + O2 reaction system. The data are also used to evaluate feasibility of the DFT methods for higher molecular weight oxy-sulfur hydrocarbons, where sulfur presents added complexity from its many valence states. The methods include: B3LYP/6-311++G(d,p), B3LYP/6-311++G(3df,2p), CCSD(T)/6-311G(d,p)//MP2/6-31G(d,p), B3P86/6-311G(2d,2p)//B3P86/6-31G(d), B3PW91/6-311++G(3df,2p), G3MP2, and CBS-QB3. The well depth for the CH3S* + 3O2 reaction to the syn-CH3SOO* adduct is found to be 9.7 kcal/mol. Low barrier exit channels from the syn-CH3SOO* adduct include: CH2S + HO2, (TS6, E(a) is 12.5 kcal/mol), CH3 + SO2 via CH3SO2 (TS2', E(a) is 17.8) and CH3SO + O (TS17, E(a) is 24.7) where the activation energy is relative to the syn-CH3SOO* stabilized adduct. The transition state (TS5) for formation of the CH3SOO adduct from CH3S* + O2 and the reverse dissociation of CH3SOO to CH3S* + O2 is relatively tight compared to typical association and simple bond dissociation reactions; this is a result of the very weak interaction. Reverse reaction is the dominant dissociation path due to enthalpy and entropy considerations. The rate constants from the chemical activation reaction and from the stabilized adduct to these products are estimated as functions of temperature and pressure. Our forward rate constant and CH3S loss profile are in agreement with the experiments under similar conditions. Of the methods above, the G3MP2 and CBS-QB3 composite methods are recommended for thermochemical determinations on these carbon-sulfur-oxygen systems, when they are feasible. 13. Histone 3 s10 phosphorylation: "caught in the R loop!". Science.gov (United States) Skourti-Stathaki, Konstantina; Proudfoot, Nicholas J 2013-11-21 In this issue of Molecular Cell, Castellano-Pozo et al. (2013) describe a connection between R loop structures and histone 3 S10 phosphorylation (H3S10P), a mark of chromatin compaction. Their results constitute a significant advance in our understanding of the role of R loops in genomic instability. 14. Superconductivity in noncentrosymmetric A g2P d3S Science.gov (United States) Yoshida, H.; Okabe, H.; Matsushita, Y.; Isobe, M.; Takayama-Muromachi, E. 2017-05-01 We have successfully synthesized the single crystal of A g2P d3S , which exhibits superconductivity with the transition temperature of Tc=2.25 K . A g2P d3S crystallizes in the space group P 4132 with the filled β -Mn structure, which has no inversion symmetry. The value of the Ginzburg-Landau parameter κGL indicates that A g2P d3S is a type-II superconductor. Δ C (Tc) /γnTc=1.50 and 2 Δ /kBTc=3.48 from the heat-capacity analyses indicate that A g2P d3S is a weak-coupling Bardeen-Cooper-Schrieffer (BCS) superconductor with an isotropic superconducting gap. On the other hand, the violation of the Werthamer-Helfand-Hohenberg curve in the H -T phase diagram implies A g2P d3S is not a typical BCS superconductor. 15. Association of Serum MiR-142-3p and MiR-101-3p Levels with Acute Cellular Rejection after Heart Transplantation Science.gov (United States) Sukma Dewi, Ihdina; Hollander, Zsuzsanna; Lam, Karen K.; McManus, Janet-Wilson; Tebbutt, Scott J.; Ng, Raymond T.; Keown, Paul A.; McMaster, Robert W.; McManus, Bruce M.; Gidlöf, Olof; Öhman, Jenny 2017-01-01 Background Identifying non-invasive and reliable blood-derived biomarkers for early detection of acute cellular rejection in heart transplant recipients is of great importance in clinical practice. MicroRNAs are small molecules found to be stable in serum and their expression patterns reflect both physiological and underlying pathological conditions in human. Methods We compared a group of heart transplant recipients with histologically-verified acute cellular rejection (ACR, n = 26) with a control group of heart transplant recipients without allograft rejection (NR, n = 37) by assessing the levels of a select set of microRNAs in serum specimens. Results The levels of seven microRNAs, miR-142-3p, miR-101-3p, miR-424-5p, miR-27a-3p, miR-144-3p, miR-339-3p and miR-326 were significantly higher in ACR group compared to the control group and could discriminate between patients with and without allograft rejection. MiR-142-3p and miR-101-3p had the best diagnostic test performance among the microRNAs tested. Serum levels of miR-142-3p and miR-101-3p were independent of calcineurin inhibitor levels, as measured by tacrolimus and cyclosporin; kidney function, as measured by creatinine level, and general inflammation state, as measured by CRP level. Conclusion This study demonstrated two microRNAs, miR-142-3p and miR-101-3p, that could be relevant as non-invasive diagnostic tools for identifying heart transplant patients with acute cellular rejection. PMID:28125729 16. Composition Design for High C3S Cement Clinker and Its Mineral Formation Institute of Scientific and Technical Information of China (English) HOU Guihua; SHEN Xiaodong; XU Zhongzi 2007-01-01 A new composition of Portland cement clinker was studied, in which KH, SM and IM was 0.98,2.4 and 2.4 respectively as well as its meal added 1%CuO (in mass). Fired at 1 200 ℃,1 350 ℃,1 400℃ and 1 450 ℃ for 30 min, the resultant mineral phases component and mineral morphology were analyzed.The performances of the cement which was made of clinker burned at 1 450 ℃ and fly ash were determined. By means of QXRD, XRD and optical microscopy, it is shown that the clinker burnt at 1 450 ℃ has the larger size crystals and distinct crystal interface, in which the C3S content is 73.37% and the mineral phases is dominantly C3S, following by minor C2S, C3A and tetracalcium aluminoferrite. The results reveal that a new type of high C3S content clinker can completely be made by traditional temperature-time schedule. The performances of the cement produced from this clinker with addtion of 50% fly ash and 5% gupsum were in agreement with the 32.5 strength grade of Portland fly-ash cement. The results also show that the clinker has a significant effect of saving energy and utilizing waste slag. 17. Computational studies of modified [Fe3S4] clusters: Why iron is optimal DEFF Research Database (Denmark) Kepp, Kasper Planeta 2008-01-01 This work reports density functional computations of metal-substituted models of biological [Fe3S4] clusters in oxidation states [MFe2S4]+/0/−1 (M = Mn, Fe, Co, Ni, Cu, Zn, and Mo). Geometry optimization with a dielectric screening model is shown to provide a substantial improvement in structure......, compared to earlier used standard procedures. The error for average Fe–S bonds decreased from 0.038 Å to 0.016 Å with this procedure. Four density functionals were compared, B3LYP, BP86, TPSS, and TPSSh. B3LYP and to a lesser extent TPSSh energies were inconsistent with experiment for the oxidized [Fe3S4......]+ cluster. BP86 (and to a slightly lesser extent TPSS) was within expected theoretical and experimental uncertainties for all oxidation states, the only qualitative error being 5 kJ/mol in favor of the MS = 3/2 configuration for the [Fe3S4]+ cluster, so BP86 was used for quantitative results. Computed... 18. Sample (S): SE3_S01 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S01 Solanum lycopersicum Lovely ai fruit Solanum lycopersicum NCBI taxonomy:408...1 Solanum lycopersicum Lovely ai (ref: http://www.mikadokyowa.com/Seed/034_tomato/034_lovely_ai/034_lovely_a 19. Crystal structure of bis{S-hexyl 3-[4-(dimethylaminobenzylidene]dithiocarbazato-κ2N3,S}copper(II Directory of Open Access Journals (Sweden) E. Zangrando 2015-06-01 Full Text Available In the title complex, [Cu(C16H24N3S22], the CuII atom is coordinated by two azomethine N and two thiolate S atoms of the chelating Schiff base ligands, resulting in a distorted square-planar coordination environment. The S—Cu—N chelating angle is of 84.41 (5°. The CuII atom is located on a crystallographic inversion centre, leading to a trans configuration of the N,S-chelating ligands. 20. ICL-induced miR139-3p and miR199a-3p have opposite roles in hematopoietic cell expansion and leukemic transformation. Science.gov (United States) Alemdehy, Mir Farshid; Haanstra, Jurgen R; de Looper, Hans W J; van Strien, Paulina M H; Verhagen-Oldenampsen, Judith; Caljouw, Yvette; Sanders, Mathijs A; Hoogenboezem, Remco; de Ru, Arnoud H; Janssen, George M C; Smetsers, Stephanie E; Bierings, Marc B; van Veelen, Peter A; von Lindern, Marieke; Touw, Ivo P; Erkeland, Stefan J 2015-06-18 Interstrand crosslinks (ICLs) are toxic DNA lesions that cause severe genomic damage during replication, especially in Fanconi anemia pathway-deficient cells. This results in progressive bone marrow failure and predisposes to acute myeloid leukemia (AML). The molecular mechanisms responsible for these defects are largely unknown. Using Ercc1-deficient mice, we show that Trp53 is responsible for ICL-induced bone marrow failure and that loss of Trp53 is leukemogenic in this model. In addition, Ercc1-deficient myeloid progenitors gain elevated levels of miR-139-3p and miR-199a-3p with age. These microRNAs exert opposite effects on hematopoiesis. Ectopic expression of miR-139-3p strongly inhibited proliferation of myeloid progenitors, whereas inhibition of miR-139-3p activity restored defective proliferation of Ercc1-deficient progenitors. Conversely, the inhibition of miR-199a-3p functions aggravated the myeloid proliferation defect in the Ercc1-deficient model, whereas its enforced expression enhanced proliferation of progenitors. Importantly, miR-199a-3p caused AML in a pre-leukemic mouse model, supporting its role as an onco-microRNA. Target genes include HuR for miR-139-3p and Prdx6, Runx1, and Suz12 for miR-199a-3p. The latter genes have previously been implicated as tumor suppressors in de novo and secondary AML. These findings show that, in addition to TRP53-controlled mechanisms, miR-139-3p and miR-199a-3p are involved in the defective hematopoietic function of ICL-repair deficient myeloid progenitors. 1. Thermodynamic studies on SrThO 3(s) Science.gov (United States) Prasad, R.; Dash, Smruti; Parida, S. C.; Singh, Ziley; Venugopal, V. 2003-01-01 The Gibbs energy of formation of SrThO 3(s) has been determined using e.m.f. and manometric techniques. In the e.m.f. method, two fluoride cells have been constructed to determine Δ fG0m(SrThO 3,s, T) using CaF 2(s) as a solid electrolyte. The cells used are: (-) O2( g), Pt/ SrO( s)+ SrF2( s)// CaF2//( SrThO3( s)+ ThO2( s)+ SrF2( s)/ Pt, O2( g)(+), ( I) (-) O2( g), Pt/ SrThO3( s)+ SrF2( s)+ ThO2( s)// CaF2( s)// CaO( s)+ CaF2( s)/ Pt, O2( g)(+). ( II) The observed e.m.f. values are represented by following respective expressions: E ( V)±0.0001=0.0998+3.254×10 -5T ( K), ( Cell I) E ( V)±0.0001=0.0285-6.37×10 -5T ( K). ( Cell II) From the measured e.m.f. values of the cells and the Δ fG0m( T) values from the literature, Δ fG0m(SrThO 3,s, T) have been calculated and are respectively given as ΔfG 0m( SrThO3, s,T)±10 kJ mol-1=-1829.2+0.2735T ( K) (978⩽T ( K)⩽1154), ( Cell I) ΔfG 0( SrThO3, s,T)±20 kJ mol-1=-1853.5+0.2867T ( K) (1008⩽T ( K)⩽1168). ( Cell II) In the manometric technique, equilibrium CO 2(g) pressures are measured over the three phase mixture: {SrThO 3(s)+SrCO 3(s)+ThO 2(s)} using a mercury manometer from 1075 to 1197 K. The corresponding Gibbs energy as a function of temperature is given by ΔfG 0m( SrThO3, s,T)( kJ mol-1)±14=-1865.4+0.3086T ( K). 2. Thermodynamic studies on SrThO{sub 3}(s) Energy Technology Data Exchange (ETDEWEB) Prasad, R. E-mail: fuelchem@magnum.barc.ernet.in; Dash, Smruti; Parida, S.C.; Singh, Ziley; Venugopal, V 2003-01-01 The Gibbs energy of formation of SrThO{sub 3}(s) has been determined using e.m.f. and manometric techniques. In the e.m.f. method, two fluoride cells have been constructed to determine {delta}{sub f}G{sup 0}{sub m}(SrThO{sub 3},s,T) using CaF{sub 2}(s) as a solid electrolyte. The cells used are: (-)O{sub 2}(g),Pt/SrO(s)+SrF{sub 2}(s)//CaF{sub 2}//SrThO{sub 3}(s)+ThO{sub 2}(s)+SrF{sub 2}(s)/Pt, O{sub 2}(g)(+), (I) (-)O{sub 2}(g),Pt/SrThO{sub 3}(s)+SrF{sub 2}(s)+ThO{sub 2}(s)//CaF{sub 2}(s)//CaO(s)+CaF{sub 2}(s)/Pt,O{sub 2}(g)= (+). (II)) The observed e.m.f. values are represented by following respective expressions: E (V){+-}0.0001=0.0998+3.254x10{sup -5}T (K), (Cell I) E (V){+-}0.0001=0.0285-6.37x10{sup -5}T (K). (Cell II) From the measured e.m.f. values of the cells and the {delta}{sub f}G{sup 0}{sub m}(T) values from the literature, {delta}{sub f}G{sup 0}{sub m}(SrThO{sub 3},s,T) have been calculated and are respectively given as {delta}{sub f}G{sup 0}{sub m}(SrThO{sub 3},s,T){+-}10 kJ mol{sup -1}=-1829.2+0.2735T (K) (978{<=}T (K){<=}1154), (Cell I)) {delta}{sub f}G{sup 0}(SrThO{sub 3},s,T){+-}20 kJ mol{sup -1}=-1853.5+0.2867T (K) (1008{<=}T (K){<=}1168). (Cell II) In the manometric technique, equilibrium CO{sub 2}(g) pressures are measured over the three phase mixture: SrThO{sub 3}(s)+SrCO{sub 3}(s)+ThO{sub 2}(s) using a mercury manometer from 1075 to 1197 K. The corresponding Gibbs energy as a function of temperature is given by {delta}{sub f}G{sup 0}{sub m}(SrThO{sub 3},s,T)(kJ mol{sup -1}){+-}14=-1865.4+0.3086T (K).)) 3. Synthesis of (3S,4R)-bengamide E Institute of Scientific and Technical Information of China (English) Qi Jun Liu; Hong Li; Shao Peng Chen; Guo Chun Zhou 2011-01-01 (3S,4R)-Bengamide E (2) was synthesized starting from D-glucono-δ-lactone (3) and the key deoxygenation step from 13 to 15 was achieved by the application of NaBH3CN and ZnI2. Compared with natural bengamide E (1), the synthetic compound (3S,4R)-bengamide E (2) was inactive against the cell growth of HUVEC and cancer cells. These data represent the significance of the stereochemistry at C-3 and C-4 of bengamides for structural recognition and binding with the target(s). 4. Sample (S): SE3_S02 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S02 Solanum lycopersicum House Momotaro fruit Solanum lycopersicum NCBI taxonom...y:4081 Solanum lycopersicum House Momotaro (ref: http://www.e-taneya.com/item/914.html) are grown at agricultural field under normal film in natural conditions. ... 5. Bond strength of W-Cu/CuCr integrated material Institute of Scientific and Technical Information of China (English) 范志康; 梁淑华; 薛旭 2001-01-01 The bond strength of W-Cu/CuCr integrated material was investigated. The results show that the fracture of W-Cu/CuCr integrated material often takes place at W-Cu/CuCr interface. Some alloying elements enhance the bond of W and CuCr alloy, which results in the increase of the strength of the W-Cu/CuCr interface. And the fracture of the WCu/CuCr integrated material occurs in the CuCr alloy part, not at the W-Cu/CuCr interface. Chromium in CuCr alloy part of the integrated material can improve Cr diffusing from the CuCr alloy to W-Cu composite and can be alloyed (near the W-Cu/CuCr interface) in the W-Cu composite. Thus the strength of W-Cu/CuCr interface is also increased. 6. Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Saria Science.gov (United States) 2009-09-15 a SN2 -like transition geometry, Figure 3c, the axial O-C-H bond angle is slightly bent more for reaction 4, Figure 3d. The products of reaction 4 are...Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Sarin Patrick F. Conforti and Matthew Braunstein* Spectral...calculations were performed on the reaction systems O(3P) + sarin and O(3P) + dimethyl methylphosphonate (DMMP), a sarin simulant. Transition state 7. Surface topography characterization of brass alloys: lead brass (CuZn39Pb3) and lead free brass (CuZn21Si3P) Science.gov (United States) Reddy, Vijeth V.; Vedantha Krishna, Amogh; Schultheiss, Fredrik; Rosén, B.-G. 2017-06-01 Manufactured surfaces usually consist of topographical features which include both those put forth by the manufacturing process, and micro-features caused by disturbances during this process. Surface characterization basically involves study of these features which influence the functionality of the surface. This article focuses on characterization of the surface topography of machined lead brass and lead free brass. The adverse effect of lead on human health and the environment has led the manufacturing sector to focus on sustainable manufacturing of lead free brass, as well as how to maintain control of the surface integrity when substituting the lead content in the brass with silicon. The investigation includes defined areal surface parameters measured on the turned samples of lead- and lead free brass using an optical coherence scanning interferometer, CSI. This paper deals with the study of surface topography of turned samples of lead- and lead free brass. It is important to study the topographical characteristics of the brass samples which are the intermediate link between the manufacturing process variables and the functional behaviour of the surface. To numerically evaluate the sample’s surface topography and to validate the measurements for a significant study, a general statistical methodology is implemented. The results indicate higher surface roughness in turned samples of lead brass compared to lead free brass. 8. Stabilization of the triphosphallyl ligand η3-P3{P(O)H} at iridium via alkaline activation of P4. Science.gov (United States) Mirabello, Vincenzo; Caporali, Maria; Gonsalvi, Luca; Manca, Gabriele; Ienco, Andrea; Peruzzini, Maurizio 2013-12-01 The selective functionalization of the polyphosphorus moiety Ph2PCH2PPh2PPPP present as a tetrahapto-ligand in complex [Ir(dppm)(Ph2PCH2PPh2PPPP)](+) (1, dppm=Ph2PCH2PPh2) was obtained by reaction of 1 with water under basic conditions at room temperature. The formation of the new triphosphaallyl moiety η(3)-P3{P(O)H} was determined in solution by NMR spectroscopy, and confirmed in the solid state by a single-crystal X-ray structure of the stable product [Ir(κ(2)-dppm)(κ(1)-dppm)(η(3)-P3{P(O)H})] (2). In solution, 2 has a fluxional behavior attributable to the four P atoms belonging to the tetraphosphorus moiety in 1 and exhibits a chemical exchange process involving the two PPh2 moieties of the same bidentate ligand, as determined by 1D and 2D NMR spectroscopy experiments carried out at variable temperature. The mechanism of the reaction was investigated at the DFT level, which suggested a selective attack of an in-situ generated OH(-) anion on one of the non-coordinated phosphorus atoms of the P4 moiety. The reaction then evolves through an acid-assisted tautomerization, which leads to the final compound 2. Bonding analysis pointed out that the new unsubstituted P3-unit in the η(3)-P3{P(O)H} moiety behaves as a triphosphallyl ligand. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. 9. Safeguards-by-Design: An Element of 3S Integration Energy Technology Data Exchange (ETDEWEB) R. S. Bean; T. A. Bjornard; D. J. Hebdich 2009-04-01 In 2008, the “20/20 Vision for the Future” background report by the IAEA Director General identified the possibility of integrating certain activities related to safeguards, safety, and security. Later in the year, the independent Commission report prepared at the request of the IAEA Director General noted that the Agency’s roles in nuclear safeguards, safety, and security (3S) complement and can mutually reinforce each other. Safeguards-by-design (SBD) is a practical measure that strengthens 3S integration, especially for the stage of nuclear facility design and construction, but also with ramifications for other stages of the facility life-cycle. This paper describes the SBD concept, with examples for diverse regulatory environments, being developed in the U.S under the U.S. Department of Energy (DOE) Next Generation Safeguards Initiative and the Advanced Fuel Cycle Initiative. This is compared with related international SBD work performed in the recent IAEA workshop on “Facility Design and Plant Operation Features that Facilitate the Implementation of IAEA Safeguards”. Potential future directions for further development of SBD and its integration within 3S are identified. 10. Circulating serum miRNA (miR-367-3p, miR-371a-3p, miR-372-3p and miR-373-3p) as biomarkers in patients with testicular germ cell cancer. Science.gov (United States) Syring, Isabella; Bartels, Joanna; Holdenrieder, Stefan; Kristiansen, Glen; Müller, Stefan C; Ellinger, Jörg 2015-01-01 Classic serum tumor markers (human chorionic gonadotropin, α1-fetoprotein and lactate dehydrogenase) have an important role in managing testicular germ cell tumor. Since only 60% of all patients with testicular germ cell tumor have elevations of these markers, there is a need for new biomarkers with greater sensitivity/specificity. miRNAs are deregulated in cancer and could serve as noninvasive serum biomarkers. We explored the role of serum miRNAs as a novel biomarker in patients with testicular germ cell tumor. Total RNA was isolated from serum. miRNA levels were quantified by quantitative real-time polymerase chain reaction. We assessed the miRNAs miR-302a-3p, 302b-3p, 302c-3p, 367-3p, 371a-3p, 372-3p and 373-3p in a subcohort of 30 patients with testicular germ cell tumor and 18 healthy subjects. Validation was performed in 76 patients treated with inguinal exploration due to suspicion of testicular germ cell tumor, of whom 59 had cancer and 17 had benign disease, and in 84 healthy male subjects. Serum miR-367-3p, 371a-3p, 372-3p and 373-3p levels were significantly increased in patients with testicular germ cell tumor compared to healthy individuals and patients with nonmalignant testicular disease. In particular miR-371a-3p allowed for sensitive (84.7%) and specific (99%) identification of patients with testicular germ cell tumor, thus, outperforming human chorionic gonadotropin or α1-fetoprotein testing. Furthermore, miR-367-3p was increased in nonseminoma compared to seminoma cases. Serum miRNA levels were increased in patients with advanced local stage and metastasis. In 9 patients with localized (clinical stage 1A) testicular germ cell tumor serum miR-371a-3p levels decreased postoperatively, indicating tumor specific release. miR-371a-3p allows for better identification of testicular germ cell tumor than α1-fetoprotein and human chorionic gonadotropin. It could be helpful for clinically managing testicular germ cell tumor, especially for monitoring 11. miR-410-3p suppresses breast cancer progression by targeting Snail. Science.gov (United States) Zhang, Ya-Feng; Yu, Yue; Song, Wang-Zhao; Zhang, Rui-Ming; Jin, Shan; Bai, Jun-Wen; Kang, Hong-Bin; Wang, Xin; Cao, Xu-Chen 2016-07-01 miR-410-3p acts as an oncogene or tumor-suppressor gene in various types of cancer. However, its role in breast cancer remains unknown. In the present study, expression of miR-410-3p in 30 breast cancer and paired adjacent normal tissues was detected by RT-qPCR. The expression of miR-410-3p was downregulated in 76.7% of the breast cancer samples. To further validate the expression of miR-410-3p in breast cancer, we analyzed miR-410-3p expression profiling data set from The Cancer Genome Atlas (TCGA) including 683 breast cancer and 87 normal breast tissues. We observed that the expression of miR-410-3p was downregulated in breast cancer tissues. Next, we investigated the influence of miR-410-3p on cell proliferation by transiently transfecting the miR-410-3p mimic or inhibitor, as well as their corresponding controls in the MDA-MB-231 and MCF7 cell lines. miR-410-3p overexpression reduced cell growth, colony formation and the number of EdU-positive cells in the MDA-MB-231 cells. In contrast, inhibition of miR-410-3p in the MCF7 cells resulted in a higher proliferation rate as assessed by MTT assay, plate colony formation and EdU assays. Furthermore, miR-410-3p inhibited epithelial-mesenchymal transition. In addition, Snail was found to be a direct target of miR-410-3p based on a luciferase assay. Overexpression of Snail was able to rescue the effect of miR-410-3p in breast cancer cells. Moreover, miR‑410-3p was inversely expressed with Snail in breast cancer samples. Our data provide new knowledge regarding the role of miR-410-3p in breast cancer progression. 12. Multiscale Hy3S: Hybrid stochastic simulation for supercomputers Directory of Open Access Journals (Sweden) Kaznessis Yiannis N 2006-02-01 Full Text Available Abstract Background Stochastic simulation has become a useful tool to both study natural biological systems and design new synthetic ones. By capturing the intrinsic molecular fluctuations of "small" systems, these simulations produce a more accurate picture of single cell dynamics, including interesting phenomena missed by deterministic methods, such as noise-induced oscillations and transitions between stable states. However, the computational cost of the original stochastic simulation algorithm can be high, motivating the use of hybrid stochastic methods. Hybrid stochastic methods partition the system into multiple subsets and describe each subset as a different representation, such as a jump Markov, Poisson, continuous Markov, or deterministic process. By applying valid approximations and self-consistently merging disparate descriptions, a method can be considerably faster, while retaining accuracy. In this paper, we describe Hy3S, a collection of multiscale simulation programs. Results Building on our previous work on developing novel hybrid stochastic algorithms, we have created the Hy3S software package to enable scientists and engineers to both study and design extremely large well-mixed biological systems with many thousands of reactions and chemical species. We have added adaptive stochastic numerical integrators to permit the robust simulation of dynamically stiff biological systems. In addition, Hy3S has many useful features, including embarrassingly parallelized simulations with MPI; special discrete events, such as transcriptional and translation elongation and cell division; mid-simulation perturbations in both the number of molecules of species and reaction kinetic parameters; combinatorial variation of both initial conditions and kinetic parameters to enable sensitivity analysis; use of NetCDF optimized binary format to quickly read and write large datasets; and a simple graphical user interface, written in Matlab, to help users 13. Sizeable beta-strength in 31Ar (beta 3p) decay DEFF Research Database (Denmark) T. Koldste, G.; Blank, B.; J. G. Borge, M. 2014-01-01 We present for the first time precise spectroscopic information on the recently discovered decay mode beta-delayed 3p-emission. The detection of the 3p events gives an increased sensitivity to the high energy part of the Gamow-Teller strength distribution from the decay of 31Ar revealing that as ... 14. Cross sections and coherence terms for associative ionization of two differently excited Na(3p) atoms NARCIS (Netherlands) Meijer, H.A.J.; Pelgrim, T.J.C.; Heideman, H.G.M.; Morgenstern, R.; Andersen, N. 1989-01-01 The associative ionization process in thermal Na(3p)-Na(3p) encounters has been studied in a series of crossed-beam experiments where the light polarization of the two laser beams preparing the excited atoms before collision was varied independently. It is shown how in this way maximum possible info 15. Terminal 3p deletions in two families--correlation between molecular karyotype and phenotype. NARCIS (Netherlands) Pohjola, P.; Leeuw, N. de; Penttinen, M.; Kaariainen, H. 2010-01-01 The 3p deletion syndrome is a rare disorder caused by deletions of different sizes in the 3p25-pter region. It is characterized by growth retardation, developmental delay, mental retardation, dysmorphism, microcephaly, and ptosis. The phenotype of individuals with deletions varies from normal to sev 16. Normal peroxisome development from vesicles induced by truncated Hansenula polymorpha Pex3p NARCIS (Netherlands) Faber, KN; Haan, GJ; Baerends, RJS; Kram, AM; Veenhuis, M 2002-01-01 We show that the synthesis of the N-terminal 50 amino acids of Pex3p (Pex3p(1-50)) in Hansenula polymorpha pex3 cells is associated with the formation of vesicular membrane structures. Biochemical and ultrastructural findings suggest that the nuclear membrane is the donor membrane compartment of 17. Integrated 3S Technology Used in Urban Grid Management Science.gov (United States) Zhao, H.; Wang, H.; Wu, W.; Wang, C. 2014-11-01 Sustainable development requires monitoring the state and changes of the city and providing the appropriate information to users anytime and anywhere. This paper takes Ningbo City as the research area, by utilizing two temporal (March 25, 2012 and November 25, 2013) ZY-3 satellite remote sensing data. 3S technology is used for urban grid management. The remote sensing information extraction of Ningbo City includes: extraction of building change information, extraction of green space change information, extraction of water information and analysis of eutrophication correspondingly. When extracting change information, we take "change information" as a special kind of "geographic information" to study the characteristics of different bands in multi-temporal data, and follow the first law of geography, namely adjacent similar principle. The extracted raster information is further converted into GIS vector format data as a basis for dynamic monitoring of Ningbo Urban Management systems: on the one hand, it can meet the demands of multi-source spatial data analysis (such as: overlay analysis, buffer analysis, etc.); on the other hand, it could meet the requirements of daily urban management. Dynamic monitoring system of Ningbo city management adopts the urban grid management mode. Based on GIS and GPS, grid management can satisfy the urban management mode -someone bear responsibility within the grid, somebody do the task under the supervision of the lattice, and at the same time play the role of remote sensing field surveying. To some extent, integrated 3S technology and urban grid management is a practical alternative of minimizing the uncertainty of remote sensing data and information extraction. With multi-scale and multi-dimensional remote sensing data, 3S integration and the urban grid management can monitor the urban state and its spatial-temporal changes. It's helpful for discovery and analysis of urban problems about resources, environment, ecology and disaster 18. The direct measurement of the 3 3P0-3 3P1 fine-structure interval and the gJ-factor of atomic silicon by laser magnetic resonance Science.gov (United States) Evenson, K. M.; Beltran-Lopez, V.; Ley-Koo, E.; Inguscio, M. 1984-01-01 The J - 1 fine structure interval and the g-factor of the 3P1 state have been determined with high precision in the present laser magnetic resonance measurements of the ground 3p2 3P multiplet of atomic Si. Delta-E(3P1-3P0) = 2,311,755.6(7) MHz, and gJ(3P1) = 1.500830(70). Single-configuration calculations of gJ for 3P1 and 3P2 yield a value for the latter which, at 1.501095, is noted to differ by an unexpectedly large margin from the experimental value. 19. Lifetime of the metastable 2/sup 3/S/sub 1/ state in stored Li/sup +/ ions Energy Technology Data Exchange (ETDEWEB) Knight, R.D. 1979-04-01 A laser-induced fluorescence technique combined with the observation of spontaneous magnetic dipole photons from the highly metastable 2/sup 3/S/sub 1/ state of Li/sup +/ was used to measure the radiative lifetime of this state. The ions are created by electron impact on a lithium atomic beam and are subsequently stored for periods of many seconds in an RF-quadrupole ion trap. A tunable dye laser excites the 2/sup 3/S--2/sup 3/P, transition at 5485A, and the intercombination electric dipole transition 2/sup 3/P/sub 1/--1 /sup 1/S/sub 0/ at 202A is observed. This process depletes the metastable population in a time tau/sub d/ < 1 sec < < tau/sub /sup 3/S/sub 1// and provides a measure of the total number of metastables. Comparison with the rate of 210A spontaneously emitted photons yields a measured value for the 2/sup 3/S/sub 1/ radiative lifetime of tau/sub rad/ = 58.6 +- 12.9 sec, where the quoted error represents 95% confidence levels. The theoretical lifetime is tau/sub theory/ = 49.0 sec. The measured value includes data taken with both /sup 6/Li/sup +/ and /sup 7/Li/sup +/ isotopes and was corrected for the slightly different detector efficiencies at 202A and 210A. A careful study of nonradiative quenching of the metastable state was necessary to understand observed differences between tau/sub rad/ and tau/sub /sup 3/S/sub 1//, the total metastable lifetime. Spatial density profiles of the ions within the trap, useful for determining the ion temperature, were obtained by scanning the laser beam horizontally across the ion trap while storing 2/sup 3/P/sub 1/--/sup 1/S/sub 0/ photon counts as a function of the laser beam's position. Agreement with a simple equilibrium model, including space charge effects, is satisfactory. A study of the optical pumping process is necessary to understand the laser-ion interaction, and observational and theoretical data are presented. 47 references. 20. The isotope effect in H3S superconductor Science.gov (United States) Szczęśniak, R.; Durajski, A. P. 2017-01-01 The experimental value of H3S a isotope coefficient decreases from 2.37 to 0.31 in the pressure range from 130 GPa to 200 GPa. We have shown that the value of 0.31 is correctly reproduced in the framework of the classical Eliashberg approach in the harmonic approximation. On the other hand, the anomalously large value of the isotope coefficient (2.37) may be associated with the strong renormalization of the normal state by the electron density of states. 1. Orientation of Zn3P2 films via phosphidation of Zn precursors Science.gov (United States) Katsube, Ryoji; Nose, Yoshitaro 2017-02-01 Orientation of solar absorber is an important factor to achieve high efficiency of thin film solar cells. In the case of Zn3P2 which is a promising absorber of low-cost and high-efficiency solar cells, (110)/(001) orientation was only reported in previous studies. We have successfully prepared (101)-oriented Zn3P2 films by phosphidation of (0001)-oriented Zn films at 350 °C. The phosphidation mechanism of Zn is discussed through STEM observations on the partially-reacted sample and the consideration of the relationship between the crystal structures of Zn and Zn3P2 . We revealed that (0001)-oriented Zn led to nucleation of (101)-oriented Zn3P2 due to the similarity in atomic arrangement between Zn and Zn3P2 . The electrical resistivity of the (101)-oriented Zn3P2 film was lower than those of (110)/(001)-oriented films, which is an advantage of the phosphidation technique to the growth processes in previous works. The results in this study demonstrated that well-conductive Zn3P2 films could be obtained by controlling orientations of crystal grains, and provide a guiding principle for microstructure control in absorber materials. 2. Electronic and Rovibrational Quantum Chemical Analysis of C_3$P$^-$: The Next Interstellar Anion? CERN Document Server Fortenberry, Ryan C 2015-01-01 C$_3$P$^-$is analogous to the known interstellar anion C$_3$N$^-$with phosphorus replacing the nitrogen in a simple step down the periodic table. In this work, it is shown that C$_3$P$^-$is likely to possess a dipole-bound excited state. It has been hypothesized and observationally supported that dipole-bound excited states are an avenue through which anions could be formed in the interstellar medium. Additionally, C$_3$P$^-$has a valence excited state that may lead to further stabilization of this molecule, and C$_3$P$^-$has a larger dipole moment than neutral C$_3$P ($\\sim 6$D vs.$\\sim 4$D). As such, C$_3$P$^-$is probably a more detectable astromolecule than even its corresponding neutral radical. Highly-accurate quantum chemical quartic force fields are also applied to C$_3$P$^-$and its singly$^{13}C substituted isotopologues in order to provide structures, vibrational frequencies, and spectroscopic constants that may aid in its detection. 3. Planarian GSK3s are involved in neural regeneration. Science.gov (United States) Adell, Teresa; Marsal, Maria; Saló, Emili 2008-02-01 Glycogen synthase kinase-3 (GSK3) is a key element in several signaling cascades that is known to be involved in both patterning and neuronal organization. It is, therefore, a good candidate to play a role in neural regeneration in planarians. We report the characterization of three GSK3 genes in Schmidtea mediterranea. Phylogenetic analysis shows that Smed-GSK3.1 is highly conserved compared to GSK3 sequences from other species, whereas Smed-GSK3.2 and Smed-GSK3.3 are more divergent. Treatment of regenerating planarians with 1-azakenpaullone, a synthetic GSK3 inhibitor, suggests that planarian GSK3s are essential for normal differentiation and morphogenesis of the nervous system. Cephalic ganglia appear smaller and disconnected in 1-azakenpaullone-treated animals, whereas visual axons are ectopically projected, and the pharynx does not regenerate properly. This phenotype is consistent with a role for Smed-GSK3s in neuronal polarization and axonal growth. 4. Crystal Structure of 3S-hydroxy-7 Melleine Science.gov (United States) Liu, Xiao-hong; Xu, Fang; Zhang, Yi; Liu, Li-hong; Huang, Hua-rong; She, Zhi-gang; Lin, Yong-cheng; Chan, Winglai 2006-10-01 A new compound, 3S-hydroxy-7 melleine was isolated from the endophytic fungus Xylariasp No.2508 from the mangrove tree on the South China Sea coast. It was the first time that this kind of compound was isolated from marine fungus. The structure was elucidated by NMR data, infrared spectrum (IR) and mass spectrometry (MS). In addition, its structure was determined by the single-crystal X-ray diffraction analysis. It crystallized in monoclinic, space group P21 with a=10.8884(19) Å, b=7.2284(13) Å, c=13.398(2) Å, β=104.217(3)°, C10H10O4·H2O, Mr=212.20, V=1022.2(3) Å3, Z=4, Dc=1.379 mg/m3, F(000)=448, μ=0.112 mm-1, the final R=0.0498, ωR=0.101 for 2407 observed reflections (I>2σ(I)). The molecular backbone of the compound includes a benzopyran ring. By comparing with the melting point and the optical rotation of the known 3R-hydroxy-7 melleine in literature, the absolute configuration of the compound was determined as 3S. It didn't exhibit antibacterial activity against Gram-positive bacterium Staphylococcus aureus at 200 μg/disk in the preliminary test. 5. Evolution of a system sensitive to stochastic noise: P3.p cell fate in Caenorhabditis. Science.gov (United States) Pénigault, Jean-Baptiste; Félix, Marie-Anne 2011-09-15 The C. elegans cell lineage is overall invariant. One rare instance of variability concerns P3.p, the most anterior vulva precursor cell, which may either fuse with the epidermis without dividing, or remain competent to form vulval tissue and divide. Here we examine the evolutionary properties of this stochastic variation in P3.p fate. In the Caenorhabditis genus, high P3.p competence is ancestral and reduction in P3.p competence and division frequency occurred in C. sp. 14 and in a clade of nine species. Within this clade, the frequency of P3.p division further varies within and among species, being intermediate in C. elegans and low in C. briggsae. P3.p fate frequency is sensitive to random mutation accumulation, suggesting that this trait may evolve rapidly because of its sensitivity to mutational impact. P3.p fate depends on LIN-39/Hox5 expression and we find that the peak of LIN-39/Hox5 protein level is displaced posteriorly in C. briggsae compared to C. elegans. However, P3.p fate specification is most sensitive to the dose of EGL-20 and CWN-1, two Wnts that are secreted in a long-range gradient from the posterior end of C. elegans larvae (accompanying article). A half-dose of either of these Wnts is sufficient to affect division frequency in C. elegans N2 to levels similar to those in C. briggsae. Symmetrically, we show that an increase in Wnt dose rescues anterior competence in C. briggsae. We propose that evolutionary variation in the concentration or interpretation of the long-range Wnt gradient may be involved in the rapid evolution of P3.p fate in Caenorhabditis. 6. Progress on the Multiphysics Capabilities of the Parallel Electromagnetic ACE3P Simulation Suite Energy Technology Data Exchange (ETDEWEB) Kononenko, Oleksiy [SLAC National Accelerator Lab., Menlo Park, CA (United States) 2015-03-26 ACE3P is a 3D parallel simulation suite that is being developed at SLAC National Accelerator Laboratory. Effectively utilizing supercomputer resources, ACE3P has become a key tool for the coupled electromagnetic, thermal and mechanical research and design of particle accelerators. Based on the existing finite-element infrastructure, a massively parallel eigensolver is developed for modal analysis of mechanical structures. It complements a set of the multiphysics tools in ACE3P and, in particular, can be used for the comprehensive study of microphonics in accelerating cavities ensuring the operational reliability of a particle accelerator. 7. Elevation of circulating miR-210-3p in high-altitude hypoxic environment Directory of Open Access Journals (Sweden) Yan eYan 2016-03-01 Full Text Available Background: The induction of miR-210-3p, a master hypoxamir, is a consistent feature of the hypoxic response in both normal and malignant cells. However, whether miR-210-3p acts as a circulating factor in response to a hypoxic environment remains unknown. The current study aimed to examine the effect of a high-altitude hypoxic environment on circulating miR-210-3p.Methods: We examined and compared the levels of miR-210-3p using TaqMan-based qRT-PCR in both peripheral blood cells and plasma from 84 ethnic Chinese Tibetans residing at 3560 m, 46 newly arrived migrant Han Chinese (Tibet Han and 82 Han Chinese residing at 8.9 m (Nanjing Han. Furthermore, we analyzed the correlations of miR-210-3p with hematological indices. Results: The relative concentrations of miR-210-3p to internal reference U6 in blood cells were significantly higher in the Tibet Han group (1.01±0.11, P<0.001 and in the Tibetan group (1.17±0.09, P<0.001 than in the Nanjing Han group (0.51±0.04. The absolute concentrations of plasma miR-210-3p were also markedly elevated in the Tibet Han group (503.54±42.95 fmol/L, P=0.004 and in the Tibetan group (557.78±39.84 fmol/L, P<0.001 compared to the Nanjing Han group (358.39±16.16 fmol/L. However, in both blood cells and plasma, miR-210-3p levels were not significantly different between the Tibet Han group and the Tibetan group (P=0.280, P=0.620, respectively. Plasma miR-210-3p concentrations were positively correlated with miR-210-3p levels in blood cells (r=0.192, P=0.005. Furthermore, miR-210-3p levels in both blood cells and plasma showed strong positive correlations with red blood cell counts and hemoglobin and hematocrit values. Conclusion: These data demonstrated, for the first time, that miR-210-3p might act as a circulating factor in response to hypoxic environments and could be associated with human adaptation to life at high altitudes. 8. Sizeable beta-strength in {sup 31}Ar (β3p) decay Energy Technology Data Exchange (ETDEWEB) Koldste, G.T. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Blank, B. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Borge, M.J.G.; Briz, J.A.; Carmona-Gallardo, M. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Fraile, L.M. [Grupo de Física Nuclear, Universidad Complutense, CEI Moncloa, E-28040 Madrid (Spain); Fynbo, H.O.U. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Giovinazzo, J. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Johansen, J.G. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Jokinen, A. [Department of Physics, University of Jyväskylä, FIN-40351 Jyväskylä (Finland); Jonson, B. [Fundamental Fysik, Chalmers Tekniska Högskola, S-41296 Göteborg (Sweden); Kurturkian-Nieto, T. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Nilsson, T. [Fundamental Fysik, Chalmers Tekniska Högskola, S-41296 Göteborg (Sweden); Perea, A.; Pesudo, V. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); and others 2014-10-07 We present for the first time precise spectroscopic information on the recently discovered decay mode β-delayed 3p-emission. The detection of the 3p events gives an increased sensitivity to the high energy part of the Gamow–Teller strength distribution from the decay of {sup 31}Ar revealing that as much as 30% of the strength resides in the β3p-decay mode. A simplified description of how the main decay modes evolve as the excitation energy increases in {sup 31}Cl is provided. 9. SOLVING THE STUDENTS’ SPEAKING PROBLEM IN DELIVERING ENGLISH PRESENTATION THROUGH 3-P TECHNIQUE Directory of Open Access Journals (Sweden) Nadif Ulfia 2017-01-01 Full Text Available Abstract: Solving the Students’ Speaking Problem in Delivering English Presentation Through 3-P Technique. The aims of this study were to (1 identify whether and to what extent 3-P Technique can improve the students’ speaking competence in delivering presentation;(2 describe the teaching and learning when 3-P technique is implemented in English class. This research was conducted at the eleventh grades of MAN 1 Bojonegoro. The subjects of the research were the teacher and the students in the academic year 2016/2017. This study applied 3 cycle action research. In collecting the data, the researcher used observations through field notes, video recording, questionnaire and interview. To analyze the quantitative data, the researcher applied descriptive statistics. Besides, to analyze qualitative data, the researcher applied constant comparative method as suggested by Garner. The findings shows that: (1 3-P Technique can improve students’ speaking competence in delivering presentation in terms of students achievement, fluency, pronunciation, ability to express their opinions, arguments, and ideas using appropriate vocabulary and grammatical form, using appropriate body language, voice control, gesture, and eye contact; and (2 3-P technique can improve classroom situation in terms of increasing students’ motivation and participation, creating live teaching atmosphere, and increasing teacher motivation. Key words: Speaking, 3 P technique, Action research Abstrak: Memecahkan Masalah Keterampilan Berbicara Siswa dalam Menyampaikan Presentasi Berbahasa Inggris Melalui Teknik 3-P. Tujuan dari penelitian ini adalah untuk (1 mengidentifikasi dan mengetahui sejauh mana teknik 3-P dapat meningkatkan kemampuan berbicara para siswa dalam menyampaikan sebuah presentasi; (2 mendeskripsikan kegiatan pembelajaran dalam penerapan teknik 3-P dalam pembelajaran Bahasa Inggris. Penelitian ini dilakukan pada kelas sebelas MAN 1 Bojonegoro. Subyek penelitian ini 10. Towards a Mg lattice clock: Observation of the^1S_{0}-^3P_{0} transition and determination of the magic wavelength CERN Document Server Kulosa, A P; Zipfel, K H; Rühmann, S; Sauer, S; Jha, N; Gibble, K; Ertmer, W; Rasel, E M; Safronova, M S; Safronova, U I; Porsev, S G 2015-01-01 We optically excite the electronic state 3s3p~^3P_{0} in ^{24}Mg atoms, laser-cooled and trapped in a magic-wavelength lattice. An applied magnetic field enhances the coupling of the light to the otherwise strictly forbidden transition. We determine the magic wavelength, the quadratic magnetic Zeeman shift and the transition frequency to be 468.463(207)\\,nm, -206.6(2.0)\\,MHz/T^2 and 655 058 646 691(101)\\,kHz, respectively. These are compared with theoretical predictions and results from complementary experiments. We also developed a high-precision relativistic structure model for magnesium, give an improved theoretical value for the blackbody radiation shift and discuss a clock based on bosonic magnesium. 11. The Application of 3P+MYSQL Technology of Dynamic Website Design%3P+MYSQL技术在动态网站设计中的运用 Institute of Scientific and Technical Information of China (English) 周悦; 朱小社 2004-01-01 在Windows2000 Server平台下分别利用ASP、PHP、JSP这三个动态网站技术,使用可以跨Windows和Linux平台的MYSQL数据库,采用三种连接数据库的方法,在三种不同特点的WEB服务器环境下,给出了动态网站设计中3P+MYSQL技术的运用. 12. Pure partial monosomy 3p (3p25.3 → pter: Prenatal diagnosis and array comparative genomic hybridization characterization Directory of Open Access Journals (Sweden) Chih-Ping Chen 2012-09-01 Conclusion: In this case, aCGH has characterized a 3p deleted region with haploinsufficiency of the neurodevelopmental genes associated with cognitive deficit and mental retardation but without involvement of the congenital heart disease susceptibility locus, and QF-PCR has determined a paternal origin of the deletion. aCGH and QF-PCR help to delineate the genomic imbalance in prenatally detected de novo chromosome aberration, and the information acquired is useful for genetic counseling. 13. Imaging performance of the hybrid pixel detectors XPAD3-S. Science.gov (United States) Brunner, F Cassol; Clemens, J C; Hemmer, C; Morel, C 2009-03-21 Hybrid pixel detectors, originally developed for tracking particles in high-energy physics experiments, have recently been used in material sciences and macromolecular crystallography. Their capability to count single photons and to apply a threshold on the photon energy suggests that they could be optimal digital x-ray detectors in low energy beams such as for small animal computed tomography (CT). To investigate this issue, we have studied the imaging performance of photon counting hybrid pixel detectors based on the XPAD3-S chip. Two detectors are considered, connected either to a Si or to a CdTe sensor, the latter being of interest for its higher efficiency. Both a standard 'International Electrotechnical Commission' (IEC) mammography beam and a beam used for mouse CT results published in the literature are employed. The detector stability, linearity and noise are investigated as a function of the dose for several imaging exposures ( approximately 0.1-400 microGy). The perfect linearity of both detectors is confirmed, but an increase in internal noise for counting statistics higher than approximately 5000 photons has been found, corresponding to exposures above approximately 110 microGy and approximately 50 microGy for the Si and CdTe sensors, respectively. The noise power spectrum (NPS), the modulation transfer function (MTF) and the detective quantum efficiency (DQE) are then measured for two energy threshold configurations (5 keV and 18 keV) and three doses ( approximately 3, 30 and 300 microGy), in order to obtain a complete estimation of the detector performances. In general, the CdTe sensor shows a clear superiority with a maximal DQE(0) of approximately 1, thanks to its high efficiency ( approximately 100%). The DQE of the Si sensor is more dependent on the radiation quality, due to the energy dependence of its efficiency its maximum is approximately 0.4 with respect to the softer radiation. Finally, we compare the XPAD3-S DQE with published curves of 14. Density functional theory of the CuA -like Cu2 S2 diamond core in Cu 2II(NGuaS)2 Cl2. Science.gov (United States) Witte, M; Gerstmann, U; Neuba, A; Henkel, G; Schmidt, W G 2016-04-30 Density functional theory (DFT) calculations with localized as well as plane-wave basis functions are performed for the recently reported dicopper thiolate species Cu2 (NGuaS)2 Cl2 [NGuaS = 2-(1,1,3,3-tetramethylguanidino) benzenethiolate, C11 H16 N3 S] and its bromo derivative [Neuba et al., Angew. Chem. Int. Ed. 2012, 51, 1714.]. For both hybrid and semilocal functionals, the neutral complexes are found to have broken symmetry (BS) character, with electron paramagnetic resonance silent, antiferromagnetically coupled [Cu(2+) …Cu(2+) ] site in which the coupling is driven by super exchange interaction within the Cu2 S2 diamond core. The accurate theoretical description of the geometric structure, however, provides a major challenge for DFT: (i) the multideterminant character of the ground state wave function has to be covered by the BS approach. It requires (ii) metageneralized gradient approximations, that is hybrid functionals with an explicit dependence on the kinetic energy of the individual orbitals: In combination with a dispersion correction, the metafunctional TPSSh results in a CuCu distance close to the experimentally observed value of 2.7 Å. For the negative charge state of the complex, a mixed-valent [Cu(1.5+) …Cu(1.5+) ] electronic structure with a smaller CuCu distance of 2.6 Å is predicted, similar to the value of the CuA site of cytochrome c oxidase. 15. Surface and interface properties of Zn3P2 solar cells Science.gov (United States) Kazmerski, L. L.; Ireland, P. J.; Catalano, A. 1981-03-01 The compositional properties of Zn3P2 thin films are studied using surface analysis techniques. Relative sensitivity factors of S(Zn sub LMM) = 0.29 and S(P sub LMM) = 0.34 are determined for this semiconductor with the aid of Auger electron spectroscopy standard spectra. The interfacial properties of the Mg/Zn3P2 thin-film solar cell are studied using Auger electron spectroscopy depth-compositional profiling in conjunction with complementary secondary ion mass spectroscopy and X-ray photoelectron spectroscopy data. Evidence is adduced for the formation of Mg3P2 at the metal-semiconductor interface. It is noted that this compound has a bandgap near that of Zn3P2. Attention is given to the possible formation of a heterojunction as a result of the interfacial reaction. 16. Newly discovered breast cancer susceptibility loci on 3p24 and 17q23.2 DEFF Research Database (Denmark) Ahmed, Shahana; Thomas, Gilles; Ghoussaini, Maya 2009-01-01 strong evidence for additional susceptibility loci on 3p (rs4973768: per-allele OR = 1.11, 95% CI = 1.08-1.13, P = 4.1 x 10(-23)) and 17q (rs6504950: per-allele OR = 0.95, 95% CI = 0.92-0.97, P = 1.4 x 10(-8)). Potential causative genes include SLC4A7 and NEK10 on 3p and COX11 on 17q.... 17. Misregulation of Scm3p/HJURP Causes Chromosome Instability in Saccharomyces cerevisiae and Human Cells OpenAIRE Prashant K. Mishra; Wei-Chun Au; John S. Choy; P Henning Kuich; Baker, Richard E.; Foltz, Daniel R.; Basrai, Munira A. 2011-01-01 The kinetochore (centromeric DNA and associated proteins) is a key determinant for high fidelity chromosome transmission. Evolutionarily conserved Scm3p is an essential component of centromeric chromatin and is required for assembly and function of kinetochores in humans, fission yeast, and budding yeast. Overexpression of HJURP, the mammalian homolog of budding yeast Scm3p, has been observed in lung and breast cancers and is associated with poor prognosis; however, the physiological relevanc... 18. Organizational Culture, 3S, and Safeguards by Design Energy Technology Data Exchange (ETDEWEB) Mladineo, Stephen V.; Frazar, Sarah L. 2012-01-31 While Safety and Security Culture are well socialized among nuclear facility designers, the concept of safeguards culture is less well defined. One area where safeguards culture may play a helpful role is in the area of Safeguards by Design. This paper will include a theoretical discussion of organizational culture, leading with safety culture and security culture that are well known, and positing that there may be room to think about safeguards culture along with the others. It will also examine the utility of the 3S concept and how this concept has been used in training for newcomer states. These will lead into a discussion of how the addition of safeguards to the mix of safety by design and security by design can be valuable, particularly as it is socialized to newcomer states. 19. Structure and Stability of Interstellar Molecule C3S Institute of Scientific and Technical Information of China (English) YU,Hai-Tao(于海涛); FU,Hong-Gang(傅宏刚); CHI,Yu-Juan(池玉娟); HUANG,Xu-Ri(黄旭日); LI,Ze-Sheng(李泽生); SUN,Jia-Zhong(孙家钟) 2002-01-01 The singlet and triplet potential energy surfaces of interstellar molecule C3S are predicted at the UB3LYP/6-311 (d) and UCCSD(T)/6-311 + G(2df) (single-point) levels. The linear singlet isomer CCCS with 1 ∑ + electronic state is found to be thermodynamically and kinetically the most stable species on the singlet surface followed by other four singiet isomers, which are unstable on the basis of calculated results. On the triplet sur face, the lowest-lying species, which lies 248.79 kJ/mol above linear singlet species CCCS, is chain CCCS connectivity with 3A' electronic state. Other four triplet isomers can be considered as unstable species by means of transition state and potential energy surface scan technologies. The structures, vibrational frequencies, dipole moments and rotational constants of all optimized species are also calculated. 20. Clock-controlled mir-142-3p can target its activator, Bmal1 Directory of Open Access Journals (Sweden) Tan Xiaochao 2012-09-01 Full Text Available Abstract Background microRNAs (miRNAs are shown to be involved in the regulation of circadian clock. However, it remains largely unknown whether miRNAs can regulate the core clock genes (Clock and Bmal1. Results In this study, we found that mir-142-3p directly targeted the 3’UTR of human BMAL1 and mouse Bmal1. The over-expression (in 293ET and NIH3T3 cells and knockdown (in U87MG cells of mir-142-3p reduced and up-regulated the Bmal1/BMAL1 mRNA and protein levels, respectively. Moreover, the expression level of mir-142-3p oscillated in serum-shocked NIH3T3 cells and the results of ChIP and luciferase reporter assays suggested that the expression of mir-142-3p was directly controlled by CLOCK/BMAL1 heterodimers in NIH3T3 cells. Conclusions Our study demonstrates that mir-142-3p can directly target the 3’UTR of Bmal1. In addition, the expression of mir-142-3p is controlled by CLOCK/BMAL1 heterodimers, suggesting a potential negative feedback loop consisting of the miRNAs and the core clock genes. These findings open new perspective for studying the molecular mechanism of circadian clock. 1. MicroRNA-139-3p indicates a poor prognosis of colon cancer. Science.gov (United States) Liu, Xiaojing; Duan, Bensong; Dong, Yuanyuan; He, Chengzhi; Zhou, Hongmei; Sheng, Haihui; Gao, Hengjun; Zhang, Xizhi 2014-01-01 MicroRNAs (miRNAs) play an important role in the regulation of gene expression and are involved in almost biological procession. Recently, miR-139-5p has been reported to be downregulated in some types of cancer, and inhibits cancer cell invasion and metastasis. However, there are few reports on the role of miR-139-3p in cancer. In this study, we examined the expression level of miR-139-3p in 63 pairs of colon cancer and adjacent paracancerous tissues using quantitative reverse transcription PCR. The levels of miR-139-3p in colon cancer tissues were significantly lower than those in adjacent noncancerous tissues. There was an inverse correlation between the level of miR-139-3p and patient's age. Lower level of miR-139-3p was significantly associated with poor overall survival, especially in patients with TNM stages I and II. In conclusion, miR-139-3p has potential as a prognostic biomarker for colon cancer. Further prospective studies are required to validate this result. 2. miR-579-3p controls melanoma progression and resistance to target therapy Science.gov (United States) Fattore, Luigi; Mancini, Rita; Acunzo, Mario; Romano, Giulia; Laganà, Alessandro; Pisanu, Maria Elena; Malpicci, Debora; Madonna, Gabriele; Mallardo, Domenico; Capone, Marilena; Fulciniti, Franco; Mazzucchelli, Luca; Botti, Gerardo; Croce, Carlo M.; Ascierto, Paolo Antonio; Ciliberto, Gennaro 2016-01-01 Therapy of melanoma patients harboring activating mutations in the BRAF (V-raf murine sarcoma viral oncogene homolog B1) oncogene with a combination of BRAF and MEK inhibitors is plagued by the development of drug resistance. Mutational events, as well as adaptive mechanisms, contribute to the development of drug resistance. In this context we uncover here the role of a miRNA, miR-579-3p. We first show that low expression of miR-579-3p is a negative prognostic factor correlating with poor survival. Expression levels of miR-579-3p decrease from nevi to stage III/IV melanoma samples and even further in cell lines resistant to BRAF/MEK inhibitors. Mechanistically, we demonstrate that miR-579-3p acts as an oncosuppressor by targeting the 3′UTR of two oncoproteins: BRAF and an E3 ubiquitin protein ligase, MDM2. Moreover miR-579-3p ectopic expression impairs the establishment of drug resistance in human melanoma cells. Finally, miR-579-3p is strongly down-regulated in matched tumor samples from patients before and after the development of resistance to targeted therapies. PMID:27503895 3. Microduplications of 3p26.3p26.2 containing CRBN gene in patients with intellectual disability and behavior abnormalities. Science.gov (United States) Papuc, Sorina M; Hackmann, Karl; Andrieux, Joris; Vincent-Delorme, Catherine; Budişteanu, Magdalena; Arghir, Aurora; Schrock, Evelin; Ţuţulan-Cuniţă, Andreea C; Di Donato, Nataliya 2015-05-01 We report on the clinical data and molecular cytogenetic findings in three unrelated patients presenting with intellectual disability and behavior abnormalities. An overlapping microduplication involving 3p26.2-26.3 was identified in these patients. All three aberrations were confirmed and proven to be parentally inherited. The sizes of the duplications were different, with a common minimal region of 423,754 bp containing two genes - TRNT1 and CRBN. Here, we hypothesize that the copy number gain of CRBN gene might be responsible for developmental delay/intellectual disability. 4. Estudio del ensanchamiento Stark en la transición 3s-3p en la serie isoelectrónica del berilio: simulaciones de dinámica molecular OpenAIRE González Herrero, Diego 2016-01-01 A pesar de los avances de los últimos años en el cálculo de espectros de ensanchamiento Stark, todavía hay ciertos asuntos que siguen sin resolverse. Uno de estos es el escalado de las líneas espectrales con la carga del emisor en las series isoelectrónicas. Incluso a día de hoy, que se conocen numerosos métodos para calcular cualquier línea espectral, este tipo de asuntos resultan muy interesantes. La estructura atómica de los emisores de una serie isoelectrónica tienen la mis... 5. Genistein up-regulates tumor suppressor microRNA-574-3p in prostate cancer. Directory of Open Access Journals (Sweden) Takeshi Chiyomaru Full Text Available Genistein has been shown to inhibit cancers both in vitro and in vivo, by altering the expression of several microRNAs (miRNAs. In this study, we focused on tumor suppressor miRNAs regulated by genistein and investigated their function in prostate cancer (PCa and target pathways. Using miRNA microarray analysis and real-time RT-PCR we observed that miR-574-3p was significantly up-regulated in PCa cells treated with genistein compared with vehicle control. The expression of miR-574-3p was significantly lower in PCa cell lines and clinical PCa tissues compared with normal prostate cells (RWPE-1 and adjacent normal tissues. Low expression level of miR-574-3p was correlated with advanced tumor stage and higher Gleason score in PCa specimens. Re-expression of miR-574-3p in PCa cells significantly inhibited cell proliferation, migration and invasion in vitro and in vivo. miR-574-3p restoration induced apoptosis through reducing Bcl-xL and activating caspase-9 and caspase-3. Using GeneCodis software analysis, several pathways affected by miR-574-3p were identified, such as 'Pathways in cancer', 'Jak-STAT signaling pathway', and 'Wnt signaling pathway'. Luciferase reporter assays demonstrated that miR-574-3p directly binds to the 3' UTR of several target genes (such as RAC1, EGFR and EP300 that are components of 'Pathways in cancer'. Quantitative real-time PCR and Western analysis showed that the mRNA and protein expression levels of the three target genes in PCa cells were markedly down-regulated with miR-574-3p. Loss-of-function studies demonstrated that the three target genes significantly affect cell proliferation, migration and invasion in PCa cell lines. Our results show that genistein up-regulates tumor suppressor miR-574-3p expression targeting several cell signaling pathways. These findings enhance understanding of how genistein regulates with miRNA in PCa. 6. Quark structure of the X(3872) and χb(3P) resonances Science.gov (United States) Ferretti, J.; Galatà, G.; Santopinto, E. 2014-09-01 We discuss the nature of the χb(3P) and X(3872) mesons. Are the χb(3P)'s standard bb¯ mesons or bb¯ states with a significative continuum component? Is the X(3872) a cc¯ state with continuum coupling effects or a meson-meson molecule? To do that, we compare quark model and unquenched quark model results for the mass barycenter and splittings of the χb(3P) multiplet. Future and more precise experimental results will discriminate between the two interpretations. In the case of the X(3872), we interpret it as a cc¯ core plus higher Fock components due to the coupling to the meson-meson continuum, and thus we think that it is compatible with the meson χc1(2P), with JPC=1++. The JPC=1++ quantum numbers are in agreement with the experimental results found by the LHCb collaboration. In our view, the X(3872)'s mass is lower than the quark model's predictions because of self-energy shifts. We also provide an estimation of the open charm/bottom strong decay modes of the X(3872) and χb(3P) mesons, such as X(3872)→DD¯* and χb2(3P)→BB¯, and radiative transitions. 7. Microstructure of interaction interface between Al-Si, Zn-Al alloys and Al2O3p/6061Al composite Institute of Scientific and Technical Information of China (English) 许志武; 闫久春; 吕世雄; 杨士勤 2004-01-01 Interaction behaviors between Al-Si, Zn-AI alloys and Al2O3p/6061AI composite at different heating temperatures were investigated. It is found that Al2O3p/6061Al composite can be wetted well by AlSi-1, AlSi-4 and Zn-Al alloys and an interaction layer forms between the alloy and composite during interaction. Little Al-Si alloys remain on the surface when they fully wet the composite and Si element in Al-Si alloy diffuses into composite entirely and assembles in the composite near the interface of Al-Si alloy/composite to form a Si-rich zone. The microstructure in interaction layer with Si penetration is still dense. Much more residual Zn-Al alloy exists on the surface of composite when it wets the composite, and porosities appear at the interface of Zn-Al alloy/composite. The penetration of elements Zn, Cu of Zn-Al alloy into composite leads to the generation of shrinkage cavities in the interaction layer and makes the microstructure of Al2 O3p/6061A1 composite loose. 8. Structural, spectroscopic, and electrochemical properties of tri- and tetradentate N3 and N3S copper complexes with mixed benzimidazole/thioether donors. Science.gov (United States) Castillo, Ivan; Ugalde-Saldívar, Víctor M; Rodríguez Solano, Laura A; Sánchez Eguía, Brenda N; Zeglio, Erica; Nordlander, Ebbe 2012-08-21 Cupric and cuprous complexes of bis(2-methylbenzimidazolyl)(2-methylthiophene)amine (L(1)), bis(2-methylbenzimidazolyl)benzylamine (L(2)), bis(2-methylbenzimidazolyl)(2,4-dimethylphenylthioethyl)amine (L(3)), bis(1-methyl-2-methylbenzimidazolyl)benzylamine (Me(2)L(2)), and bis(1-methyl-2-methylbenzimidazolyl)(2,4-dimethylphenylthioethyl)amine (Me(2)L(3)) have been spectroscopically, structurally, and electrochemically characterised. The thioether-containing ligands L(3) and Me(2)L(3) give rise to complexes with Cu-S bonds in solution and in the solid state, as evidenced by UV-vis spectroscopy and X-ray crystallography. The Cu(2+) complexes [L(1)CuCl(2)] (1), [L(2)CuCl(2)] (2) and [Me(2)L(3)CuCl]ClO(4) (3(Me,ClO4)) are monomeric in solution according to ESI mass spectrometry data, as well as in the solid state. Their Cu(+) analogues [L(1)Cu]ClO(4), [L(2)Cu]ClO(4), [L(3)Cu]ClO(4) (4-6), [BOC(2)L(1)Cu(NCCH(3))]ClO(4) (4(BOC)), [Me(2)L(2)Cu(NCCH(3))(2)]PF(6) (5(Me)) and [Me(2)L(3)Cu](2)(ClO(4))(2) (6(Me)) are also monomeric in acetonitrile solution, as confirmed crystallographically for 4(BOC) and 5(Me). In contrast, 6(Me) is dimeric in the solid state, with the thioether group of one of the ligands bound to a symmetry-related Cu(+) ion. Cyclic voltammetry studies revealed that the bis(2-methylbenzimidazolyl)amine-Cu(2+)/Cu(+) systems possess half-wave potentials in the range -0.16 to -0.08 V (referenced to the ferrocenium-ferrocene couple); these values are nearly 0.23 V less negative than those reported for related bis(picolyl)amine-derived ligands. Based on these observations, the N(3) or N(3)S donor set of the benzimidazole-derived ligands is analogous to previously reported chelating systems, but the electronic environment they provide is unique, and may have relevance to histidine and methionine-containing metalloenzymes. This is also reflected in the reactivity of [Me(2)L(2)Cu(NCCH(3))(2)](+) (5(Me)) and [Me(2)L(3)Cu](+) (6(Me)) towards dioxygen, which results 9. Doubly excited 2s2p 1,3p1 resonances in photoionization of helium Institute of Scientific and Technical Information of China (English) Wan Jian-Jie; Dong Chen-Zhong 2009-01-01 The multi-configuration Dirac-Fock (MCDF) method is implemented to study doubly excited 2s2p 1,3P1 resonances of the helium atom and the interference between photoionization and photoexcitation autoionization processes.In order to reproduce the total photoionization sprectra,the excited energies from the ground ls2 1 S0 state to the doubly excited 2s2p 1'3P1 states and the relevant Auger decay rates and widths are calculated in detail. Furthermore,the interference profile determined by the so-called Fano parameters q and p2 is also reproduced. Good agreement is found between the present results and other available theoretical and experimental results. This indeed shows a promising way to investigate the Fano resonances in photoionization of atoms within the MCDF scheme,although there are some discrepancies in the present calculations of the 2s2p 3P1 state. 10. Magnetic Properties of Quantized Vortices in Neutron ^3P_2 Superfluids in Neutron Stars CERN Document Server Masuda, Kota 2015-01-01 We discuss quantized vortices in neutron ^3P_2 superfluids, which are believed to realize in high density neutron matter such as neutron stars. By using the Ginzburg-Landau free energy for ^3P_2 superfluids, we determine the ground state in the absence and presence of the external magnetic field, and numerically construct ^3P_2 quantized vortices in the absence and presence of the external magnetic field along the vortex axis (poloidal) or angular direction (toroidal). We find in certain situations the spontaneous magnetization of the vortex core, whose typical magnitude is about 10^{7-8} Gauss, but the net magnetic field in a neutron star is negligible because of the ratio of the vortex core size \\sim 10fm and the intervortex distance \\sim 10^{-6}m in a vortex lattice. 11. A new form of Ca3P2 with a ring of Dirac nodes Directory of Open Access Journals (Sweden) Lilia S. Xie 2015-08-01 Full Text Available We report the synthesis and crystal structure of a new high-temperature form of Ca3P2. The crystal structure was determined through Rietveld refinements of synchrotron powder x-ray diffraction data. This form of Ca3P2 has a crystal structure of the hexagonal Mn5Si3 type, with a Ca ion deficiency compared to the ideal 5:3 stoichiometry. This yields a stable, charge-balanced compound of Ca2+ and P3−. We also report the observation of a secondary hydride phase, Ca5P3H, which again is a charge-balanced compound. The calculated band structure of Ca3P2 indicates that it is a three-dimensional Dirac semimetal with a highly unusual ring of Dirac nodes at the Fermi level. The Dirac states are protected against gap opening by a mirror plane in a manner analogous to what is seen for graphene. 12. Experimental and theoretical study of 3p photoionization and subsequent Auger decay in atomic chromium Science.gov (United States) Keskinen, J.; Huttula, S.-M.; Mäkinen, A.; Patanen, M.; Huttula, M. 2015-12-01 3p photoionization and subsequent low kinetic energy Coster-Kronig and super Coster-Kronig Auger decay have been studied in atomic chromium. The binding energies, line widths, and relative intensities for the transitions seen in the synchrotron radiation excited 3p photoelectron spectrum are determined. The high resolution M2,3 M4,5 M4,5 and M2,3 M4,5 N1 Auger electron spectra following the electron impact excited 3p ionization are presented and the kinetic energies, relative intensities, and identifications are given for the main lines. The experimental findings are compared with the theoretical predictions obtained from Hartree-Fock and multiconfiguration Dirac-Fock approaches. 13. Domain architectures of the Scm3p protein provide insights into centromere function and evolution OpenAIRE Aravind, L.; Lakshminarayan, M. Iyer; Wu, Carl 2007-01-01 Recently, Scm3p has been shown to be a nonhistone component of centromeric chromatin that binds stoichiometrically to CenH3–H4 histones, and to be required for the assembly of kinetochores in S. cerevisiae. Scm3p is conserved across fungi, and displays a remarkable variation in protein size, ranging from ~200 amino acids in Saccharomyces cerevisiae to ~1300 amino acids in Neurospora crassa. This is primarily due a variable C-terminal segment that is linked to a conserved N-terminal, CenH3-int... 14. Autism spectrum disorder, Klinefelter syndrome, and chromosome 3p21.31 duplication: a case report. Science.gov (United States) Stuart, Scott W; King, Casey H; Pai, G Shashidar 2007-12-18 Autism spectrum disorders are heterogeneous in nature with idiopathic and genetic origins. We present a 7-year-old boy with a long history of multiple behavioral concerns, poor school performance, repetitive/compulsive tendencies, poor social skills, and language delays. A multidisciplinary evaluation concluded that the patient met full criteria for autism. A genetic evaluation demonstrated Klinefelter syndrome 47, XXY karyotype with concurrent duplication of 3p21.31 by microarray analysis. Maternal genetic analysis demonstrated the same 3p21.31 duplication. The potential implication with regard to autism spectrum disorders has not been previously discussed in the literature. 15. Tetra-μ-acetato-κ8O:O′-bis{[2-methylsulfanyl-4-(pyridin-4-yl-κNpyrimidine]copper(II}(Cu—Cu Directory of Open Access Journals (Sweden) Hai-Bin Zhu 2011-08-01 Full Text Available The binuclear title compound, [Cu2(CH3CO24(C10H9N3S2], comprises a Cu2(CH3CO24 paddle-wheel core axially bound by two 2-methylsulfanyl-4-(pyridin-4-ylpyrimidine ligands. The complex molecule has an inversion center lying at the mid-point of the Cu—Cu bond. 16. The infinite order point on Y~2=X~3-p~3 Institute of Scientific and Technical Information of China (English) 张绍伟 1995-01-01 The parameterization of the elliptic curve Y2=X2+1 is given by using the modular forms of X(12) Then using class field theory over imaginary quadratic field and Shimura reciprocity, an infinite order point on the curve y2- = x3-p3 is constructed, for prime p=7 (mod 24). 17. Using P3P in a web services-based context-aware application platform NARCIS (Netherlands) Zuidweg, M.; Halasz, E.; Goncalves Filho, J.; Lukovszki, C.; Marosits, T.; van Sinderen, Marten J. This paper describes a proposal for a privacy control architecture to be applied in the WASP project. The WASP project aims to develop a context-aware service platform on top of 3G networks, using web services technology. The proposed privacy control architecture is based on the P3P privacy policy 18. MicroRNA-142-3p Negatively Regulates Canonical Wnt Signaling Pathway. Directory of Open Access Journals (Sweden) Tanyu Hu Full Text Available Wnt/β-catenin signaling pathway plays essential roles in mammalian development and tissue homeostasis. MicroRNAs (miRNAs are a class of regulators involved in modulating this pathway. In this study, we screened miRNAs regulating Wnt/β-catenin signaling by using a TopFlash based luciferase reporter. Surprisingly, we found that miR-142 inhibited Wnt/β-catenin signaling, which was inconsistent with a recent study showing that miR-142-3p targeted Adenomatous Polyposis Coli (APC to upregulate Wnt/β-catenin signaling. Due to the discordance, we elaborated experiments by using extensive mutagenesis, which demonstrated that the stem-loop structure was important for miR-142 to efficiently suppress Wnt/β-catenin signaling. Moreover, the inhibitory effect of miR-142 relies on miR-142-3p rather than miR-142-5p. Further, we found that miR-142-3p directly modulated translation of Ctnnb1 mRNA (encoding β-catenin through binding to its 3' untranslated region (3' UTR. Finally, miR-142 was able to repress cell cycle progression by inhibiting active Wnt/β-catenin signaling. Thus, our findings highlight the inhibitory role of miR-142-3p in Wnt/β-catenin signaling, which help to understand the complex regulation of Wnt/β-catenin signaling. 19. Linkage of atopic dermatitis to chromosomes 4q22, 3p24 and 3q21 DEFF Research Database (Denmark) Christensen, Ulla; Møller-Larsen, Steffen; Nyegaard, Mette 2009-01-01 Atopic dermatitis (AD) is a common, itchy skin disease of complex inheritance characterized by dermal and epidermal inflammation. The heritability is considerable and well documented. To date, four genome scans have examined the AD phenotype, showing replicated linkage at 3p26-22, 3q13-21 and 18q11... 20. Baryonic {sup 3}P{sub 2} superfluidity under charged-pion condensation with {delta} isobar Energy Technology Data Exchange (ETDEWEB) Takatsuka, T.; Tamagaki, R. [Iwate Univ., Morioka, Iwate (Japan) 1999-08-01 We study the baryonic {sup 3}P{sup 2} superfluidity under charged-pion condensation with isobar ({delta}) degrees of freedom. After a remark on motivations of the present study, the outline of theoretical framework is briefly described, typical results of the superfluid critical temperature are shown, and the possibility of coexistence of the superfluid with charged-pion condensation is discussed. (author) 1. Construction of a consistent YAC contig for human chromosome region 3p14.1 NARCIS (Netherlands) Bardenheuer, W; Michaelis, S; Lux, A; Vieten, L; Brocker, F; Julicher, K; Willers, C; Siebert, R; Smith, DI; vanderHout, AH; Buys, C; Schutte, J; Opalka, B 1996-01-01 Chromosomal deletions and translocations of human chromosome region 3p14 are observed in various human malignancies and suggest the existence of a tumor suppressor gene locus within this region. Tumors most frequently affected by these aberrations are small-cell lung cancer and renal-cell carcinoma. 2. Using P3P in a web services-based context-aware application platform NARCIS (Netherlands) Zuidweg, M.; Goncalves Filho, J.; Sinderen, van M.J.; Halasz, E.; Lukovszki, C.; Marosits, T. 2003-01-01 This paper describes a proposal for a privacy control architecture to be applied in the WASP project. The WASP project aims to develop a context-aware service platform on top of 3G networks, using web services technology. The proposed privacy control architecture is based on the P3P privacy policy d 3. Repumping of ultracold strontium atoms using the ^3P2 - ^3D2 transition Science.gov (United States) Mickelson, P. G.; Martinez de Escobar, Y. N.; Traverso, A. J.; Killian, T. C. 2008-05-01 We discuss recent experiments involving ultracold strontium. Using a commercially-available 3 micron laser, we repump atoms out of the ^3P2 level via the ^3D2 state and gain almost a factor of 10 in the number of atoms in our system. This increase in the signal-to-noise ratio enables improved spectroscopy of strontium in our optical trap. 4. miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation DEFF Research Database (Denmark) Qiu, Weimin; Kassem, Moustapha 2014-01-01 Wnt signaling determines human stromal (mesenchymal) stem cell (hMSC) differentiation fate into the osteoblast or adipocyte lineage. microRNAs (miRNAs) are small RNA molecules of 21-25 nucleotides that regulate many aspects of osteoblast biology. Thus, we examined miRNAs regulated by Wnt signaling...... in hMSC. We identified miRNA (miR)-141-3p as a Wnt target which in turn inhibited Wnt signaling. Moreover, miR-141-3p inhibited hMSC proliferation by arresting cells at the G1 phase of the cell cycle. miR-141-3p inhibited osteoblast differentiation of hMSC as evidenced by reduced alkaline phosphatase...... activity, gene expression and in vitro mineralized matrix formation. Bioinformatic studies, Western blot analysis and 3'UTR reporter assay demonstrated that cell division cycle 25A (CDC25A) is a direct target of miR-141-3p. siRNA-mediated knock-down of CDC25A inhibited hMSC proliferation and osteoblast... 5. Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Sarin Science.gov (United States) Conforti, Patrick F.; Braunstein, Matthew; Dodd, James A. 2009-10-01 Electronic structure and molecular dynamics calculations were performed on the reaction systems O(3P) + sarin and O(3P) + dimethyl methylphosphonate (DMMP), a sarin simulant. Transition state geometries, energies, and heats of reaction for the major reaction pathways were determined at several levels of theory, including AM1, B3LYP/6-311+G(d,p), and CBS-QB3. The major reaction pathways for both systems are similar and include H-atom abstraction, H-atom elimination, and methyl elimination, in rough order from low to high energy. The H-atom abstraction channels have fairly low barriers (˜10 kcal mol-1) and are close to thermoneutral, while the other channels have relatively high energy barriers (>40 kcal mol-1) and a wide range of reaction enthalpies. We have also found a two-step pathway leading to methyl elimination through O-atom attack on the phosphorus atom for DMMP and sarin. For sarin, the two-step methyl elimination pathway is significantly lower in energy than the single-step pathway. We also present results of O(3P) + sarin and O(3P) + DMMP reaction cross sections over a broad range of collision energies (2-10 km s-1 collision velocities) obtained using the direct dynamics method with an AM1 semiempirical potential. These excitation functions are intended as an approximate guide to future hyperthermal measurements, which to our knowledge have not yet examined either of these systems. The reaction barriers, reaction enthalpies, transition state structures, and excitation functions are generally similar for DMMP and sarin, with some moderate differences for methyl elimination energetics, which indicates DMMP will likely be a good substitute for sarin in many O(3P) chemical investigations. 6. Photoelectric characteristics of CH3NH3PbI3/p-Si heterojunction Science.gov (United States) Yamei, Wu; Ruixia, Yang; Hanmin, Tian; Shuai, Chen 2016-05-01 Organic-inorganic hybrid perovskite CH3NH3PbI3 film is prepared on p-type silicon substrate using the one-step solution method to form a CH3NH3PbI3/p-Si heterojunction. The film morphology and structure are characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The photoelectric properties of the CH3NH3PbI3/p-Si heterojunction are studied by testing the current-voltage (I-V) with and without illumination and capacitance-voltage (C-V) characteristics. It turns out from the I-V curve without illumination that the CH3NH3PbI3/p-Si heterojunction has a rectifier feature with the rectification ratio over 70 at the bias of ±5 V. Also, there appears a photoelectric conversion phenomenon on this heterojunction with a short circuit current (Isc) of 0.16 μA and an open circuit voltage (Voc) of about 10 mV The high frequency C-V characteristic of the Ag/CH3NH3PbI3/p-Si heterojunction turns out to be similar to that of the metal-insulator-semiconductor (MIS) structure, and a parallel translation of the C-V curve along the forward voltage axis is found. This parallel translation means the existence of defects at the CH3NH3PbI3/p-Si interface and positive fixed charges in the CH3NH3PbI3 layer. The defects at the interface of the CH3NH3PbI3/p-Si heterojunction result in the dramatic decline of the Voc. Besides, the C-V test of CH3NH3PbI3 film shows a non-linear dielectric property and the dielectric value is about 4.64 as calculated. Project supported by the Hebei Province Natural Science Foundation of China (No. F2014202184) and the Tianjin Natural Science Foundation of China (No. 15JCZDJC37800). 7. Photoexcitation and photoionization from the 2p{sup 5}3p[5/2]{sub 2,3} levels in neon Energy Technology Data Exchange (ETDEWEB) Baig, M. A.; Bokhari, I. A.; Rafiq, M.; Kalyar, M. A.; Hussian, T.; Ali, Raheel; Piracha, N. K. [Atomic and Molecular Physics Laboratory, Department of Physics, Quaid-i-Azam University, 45320 Islamabad (Pakistan); Department of Physics, John Carroll University, 20700 N. Park Boulevard, University Heights, Ohio 44118 (United States) 2011-07-15 We present measurements of the excitation spectra from the 2p{sup 5}3p[5/2]{sub 3,2} levels in neon using two-step laser excitation and ionization in conjunction with an optogalvanic detection in dc and rf discharge cells. The 2p{sup 5}3p[5/2]{sub 3,2} intermediate levels have been approached via the collisionally populated 2p{sup 5}3s[3/2]{sub 2} metastable level. The Rydberg series 2p{sup 5}({sup 2}P{sub 3/2})nd[7/2]{sub 4} (12 {<=}n{<=} 44), 2p{sup 5}({sup 2}P{sub 3/2})ns[3/2]{sub 2} (13 {<=}n{<=} 35) and the parity forbidden transitions 2p{sup 5}({sup 2}P{sub 3/2})np[5/2]{sub 3} (13 {<=}n{<=} 19) have been observed from the 2p{sup 5}3p[5/2]{sub 3} level, whereas the 2p{sup 5}({sup 2}P{sub 3/2})nd[7/2]{sub 3} (12 {<=}n{<=} 44), 2p{sup 5}({sup 2}P{sub 3/2})ns[3/2]{sub 2} (13 {<=}n{<=} 35), and 2p{sup 5}({sup 2}P{sub 1/2})nd'[5/2]{sub 3} (9 {<=}n{<=} 12) Rydberg series have been observed from the 2p{sup 5}3p[5/2]{sub 2} level in accordance with the {Delta}J ={Delta}K = {+-} 1 selection rules. The photoionization cross sections from the 2p{sup 5}3p[5/2]{sub 3} intermediate level have been measured at eight ionizing laser wavelengths (399, 395, 390, 385, 380, 370, 364, and 355 nm) and that from the 2p{sup 5}3p[5/2]{sub 2} level at 401.8 nm. These measurements are in excellent agreement with the experimental values reported in the literature, while the experimental data lie much below the theoretically calculated photoionization cross sections curve. 8. Cu-67 Photonuclear Production Science.gov (United States) Starovoitova, Valeriia; Foote, Davy; Harris, Jason; Makarashvili, Vakhtang; Segebade, Christian R.; Sinha, Vaibhav; Wells, Douglas P. 2011-06-01 Cu-67 is considered as one of the most promising radioisotopes for cancer therapy with monoclonal antibodies. Current production schemes using high-flux reactors and cyclotrons do not meet potential market need. In this paper we discuss Cu-67 photonuclear production through the reaction Zn-68(γ,p)Cu-67. Computer simulations were done together with experiments to study and optimize Cu-67 yield in natural Zn target. The data confirms that the photonuclear method has potential to produce large quantities of the isotope with sufficient purity to be used in medical field. 9. Verifier-based Password Authenticated 3P-EKE Protocol using PCLA keys Directory of Open Access Journals (Sweden) Archana Raghuvamshi 2016-06-01 Full Text Available This paper endeavors to present a novel framework for the generic structure of a verifier-based password authenticated Three-Party Encrypted Key Exchange (3P-EKE protocol which yields more efficient protocol than the ones knew before. A previous framework presented by Archana and Premchand is more secured against all types of attacks like password guessing, replay, pre-play, man-in-the-middle attack etc. But unfortunately, this protocol does not solve the problem of a server compromise. These proofs help as inspiration to search for another framework. The framework we offer produces more efficient 3P-EKE protocol, and, in addition, delivers perceptive clarification about the existing attacks that do not solve in the previous framework. Moreover, it allows direct change from a class of verge private-key encryption to a hybrid (symmetric & Asymmetric one without significant overhead. 10. Multi-reassortant G3P[3] group A rotavirus in a horseshoe bat in Zambia. Science.gov (United States) Sasaki, Michihito; Orba, Yasuko; Sasaki, Satoko; Gonzalez, Gabriel; Ishii, Akihiro; Hang'ombe, Bernard M; Mweene, Aaron S; Ito, Kimihito; Sawa, Hirofumi 2016-10-01 Group A rotavirus is a major cause of diarrhoea in humans, especially in young children. Bats also harbour group A rotaviruses, but the genetic backgrounds of bat rotavirus strains are usually distinct from those of human rotavirus strains. We identified a new strain of group A rotavirus in the intestinal contents of a horseshoe bat in Zambia. Whole genome sequencing revealed that the identified virus, named RVA/Bat-wt/ZMB/LUS12-14/2012/G3P[3], possessed the genotype constellation G3-P[3]-I3-R2-C2-M3-A9-N2-T3-E2-H3. Several genome segments of LUS12-14 were highly similar to those of group A rotaviruses identified from humans, cows and antelopes, indicating interspecies transmission of rotaviruses between bats and other mammals with possible multiple genomic reassortment events. 11. The Participative Design of an Endoscopy Facility using Lean 3P. Science.gov (United States) Smith, Iain 2016-01-01 In the UK, bowel cancer is the second largest cancer killer. Diagnosing people earlier can save lives but demand for endoscopies is increasing and this can put pressure on waiting times. To address this challenge, an endoscopy unit in North East England decided to improve their facilities to increase capacity and create environments that improve the experience of users. This presented a significant opportunity for step change improvement but also a problem in terms of creating designs that meet user requirements whilst addressing structural or space constraints. The Lean design process known as '3P' (standing for the production preparation process) was utilised as a participative design strategy to engage stakeholders in the design of the new department. This involved a time-out workshop (or 3P event) in which Lean and participative design tools were utilised to create an innovative design based on 'point of delivery' (POD) principles. The team created a design that demonstrated an increase in treatment room capacity by 25% and bed capacity by 70% whilst reducing travel distance for patients by 25.8% and staff by 27.1%. This was achieved with an increase in available space of only 13%. The Lean 3P method provided a structured approach for corporate and clinical staff to work together with patient representatives as cross-functional teams. This participative approach facilitated communication and learning between stakeholders about care processes and personal preferences. Lean 3P therefore appears to be a promising approach to improving the healthcare facilities design process to meet user requirements. 12. Temperature dependence and mechanism of the reaction between O(3P) and chlorine dioxide Science.gov (United States) Colussi, A. J.; Sander, S. P.; Fiedl, R. R. 1992-01-01 Second-order rate constants for the decay of O(3P) in excess chlorine dioxide, k(II), were measured as a function of total pressure (20-600 Torr argon) and temperature (248-312 K), using flash photolysis-atomic resonance fluorescence. Results indicate that k(II) is pressure dependent with a value, K(b), that is nonzero at zero pressure, and both the third-order rate constant and k(b) have negative temperature dependences. 13. LiNbO3/p+n diode surface acoustic wave memory correlator Institute of Scientific and Technical Information of China (English) 张朝; 水永安; 印建华 1997-01-01 A detailed theoretical analysis of strip-coupled LiNbO3/p+ n diode surface acoustic wave (SAW) memory correlator in the parametric mode is presented. The influence of some important factors on correlation output is analyzed and calculated, including the amplitudes of reference, read and write signal, duration of write signal and doping density of the diode array. The conclusions can be employed for the design of improved strip-coupled SAW memorycorrelators. 14. High-energy single diffractive dissociation of nucleons and the 3P-model applicability range Energy Technology Data Exchange (ETDEWEB) Godizov, A.A., E-mail: anton.godizov@gmail.com 2016-11-15 The adequacy of the triple-Pomeron interaction approximation (the 3P-model) for description of the high-energy single diffractive dissociation of nucleons is analyzed via application to the available experimental data on nucleon–nucleon scattering, including the recent results produced by CMS Collaboration which allow to estimate reliably the triple-Pomeron coupling value. It is argued that the total contribution of secondary Reggeon exchanges is not negligible up to the Tevatron energy. 15. Operationalising the Lean principles in maternity service design using 3P methodology Science.gov (United States) Smith, Iain 2016-01-01 The last half century has seen significant changes to Maternity services in England. Though rates of maternal and infant mortality have fallen to very low levels, this has been achieved largely through hospital admission. It has been argued that maternity services may have become over-medicalised and service users have expressed a preference for more personalised care. NHS England's national strategy sets out a vision for a modern maternity service that continues to deliver safe care whilst also adopting the principles of personalisation. Therefore, there is a need to develop maternity services that balance safety with personal choice. To address this challenge, a maternity unit in North East England considered improving their service through refurbishment or building new facilities. Using a design process known as the production preparation process (or 3P), the Lean principles of understanding user value, mapping value-streams, creating flow, developing pull processes and continuous improvement were applied to the design of a new maternity department. Multiple stakeholders were engaged in the design through participation in a time-out (3P) workshop in which an innovative pathway and facility for maternity services were co-designed. The team created a hybrid model that they described as “wrap around care” in which the Lean concept of pull was applied to create a service and facility design in which expectant mothers were put at the centre of care with clinicians, skills, equipment and supplies drawn towards them in line with acuity changes as needed. Applying the Lean principles using the 3P method helped stakeholders to create an innovative design in line with the aspirations and objectives of the National Maternity Review. The case provides a practical example of stakeholders applying the Lean principles to maternity services and demonstrates the potential applicability of the Lean 3P approach to design healthcare services in line with policy requirements 16. Fracture toughness of Al-4%Mg/Al{sub 2}O{sub 3}p composites Energy Technology Data Exchange (ETDEWEB) Pestes, R.H. [Hewlett-Packard, Camus, WA 98607 (United States); Kamat, S.V. [Mechanical and Materials Engineering Department, Washington State University, Pullman, WA 99164-2920 (United States); Hirth, J.P. [Mechanical and Materials Engineering Department, Washington State University, Pullman, WA 99164-2920 (United States) 1994-12-20 Fracture toughness tests were carried out on Al-4%Mg/Al{sub 2}O{sub 3}p composites containing different sizes and volume fractions of alumina particulates. The results indicated that the fracture toughness was dependent on the interparticle spacing provided the particulate size was below a critical size. The critical particle size corresponds to the situation wherein the energy release rate on particulate cracking is sufficient to trigger dynamic unstable crack extension. ((orig.)) 17. Operationalising the Lean principles in maternity service design using 3P methodology. Science.gov (United States) Smith, Iain 2016-01-01 The last half century has seen significant changes to Maternity services in England. Though rates of maternal and infant mortality have fallen to very low levels, this has been achieved largely through hospital admission. It has been argued that maternity services may have become over-medicalised and service users have expressed a preference for more personalised care. NHS England's national strategy sets out a vision for a modern maternity service that continues to deliver safe care whilst also adopting the principles of personalisation. Therefore, there is a need to develop maternity services that balance safety with personal choice. To address this challenge, a maternity unit in North East England considered improving their service through refurbishment or building new facilities. Using a design process known as the production preparation process (or 3P), the Lean principles of understanding user value, mapping value-streams, creating flow, developing pull processes and continuous improvement were applied to the design of a new maternity department. Multiple stakeholders were engaged in the design through participation in a time-out (3P) workshop in which an innovative pathway and facility for maternity services were co-designed. The team created a hybrid model that they described as "wrap around care" in which the Lean concept of pull was applied to create a service and facility design in which expectant mothers were put at the centre of care with clinicians, skills, equipment and supplies drawn towards them in line with acuity changes as needed. Applying the Lean principles using the 3P method helped stakeholders to create an innovative design in line with the aspirations and objectives of the National Maternity Review. The case provides a practical example of stakeholders applying the Lean principles to maternity services and demonstrates the potential applicability of the Lean 3P approach to design healthcare services in line with policy requirements. 18. Configuration interaction calculations of positron binding to Be({sup 3}P ) Energy Technology Data Exchange (ETDEWEB) Bromley, M.W.J. [Department of Physics, San Diego State University, San Diego, CA 92182 (United States)]. E-mail: mbromley@physics.sdsu.edu; Mitroy, J. [Faculty of Technology, Charles Darwin University, Darwin, NT 0909 (Australia)]. E-mail: jxm107@rsphysse.anu.edu.au 2006-06-15 The configuration interaction method is applied to investigate the possibility of positron binding to the metastable beryllium (1s{sup 2}2s2p {sup 3}P ) state. The largest calculation obtained an estimated energy that was unstable by 0.00014 Hartree with respect to the Ps + Be{sup +}(2s) lowest dissociation channel. It is likely that positron binding to parent states with non-zero angular momentum is inhibited by centrifugal barriers. 19. ^3P_2-^3F_2 Pairing in Dense Neutron Matter The Spectrum of Solutions CERN Document Server Zverev, M V; Khodel, V A 2003-01-01 The ^3P_2-^3F_2 pairing model is generally considered to provide an adequate description of the superfluid states of neutron matter at densities some 2-3 times that of saturated symmetrical nuclear matter. The problem of solving the system of BCS gap equations expressing the ^3P_2-^3F_2 model is attacked with the aid of the separation approach. This method, developed originally for quantitative study of S-wave pairing in the presence of strong short-range repulsions, serves effectively to reduce the coupled, singular, nonlinear BCS integral equations to a set of coupled algebraic equations. For the first time, sufficient precision becomes accessible to resolve small energy splittings between the different pairing states. Adopting a perturbative strategy, we are able to identify and characterize the full repertoire of real solutions of the ^3P_2-^3F_2 pairing model, in the limiting regime of small tensor-coupling strength. The P-F channel coupling is seen to lift the striking parametric degeneracie... 20. Ypq3p-dependent histidine uptake by the vacuolar membrane vesicles of Saccharomyces cerevisiae. Science.gov (United States) Manabe, Kunio; Kawano-Kawada, Miyuki; Ikeda, Koichi; Sekito, Takayuki; Kakinuma, Yoshimi 2016-06-01 The vacuolar membrane proteins Ypq1p, Ypq2p, and Ypq3p of Saccharomyces cerevisiae are known as the members of the PQ-loop protein family. We found that the ATP-dependent uptake activities of arginine and histidine by the vacuolar membrane vesicles were decreased by ypq2Δ and ypq3Δ mutations, respectively. YPQ1 and AVT1, which are involved in the vacuolar uptake of lysine/arginine and histidine, respectively, were deleted in addition to ypq2Δ and ypq3Δ. The vacuolar membrane vesicles isolated from the resulting quadruple deletion mutant ypq1Δypq2Δypq3Δavt1Δ completely lost the uptake activity of basic amino acids, and that of histidine, but not lysine and arginine, was evidently enhanced by overexpressing YPQ3 in the mutant. These results suggest that Ypq3p is specifically involved in the vacuolar uptake of histidine in S. cerevisiae. The cellular level of Ypq3p-HA(3) was enhanced by depletion of histidine from culture medium, suggesting that it is regulated by the substrate. 1. miR-142-3p prevents macrophage differentiation during cancer-induced myelopoiesis. Science.gov (United States) Sonda, Nada; Simonato, Francesca; Peranzoni, Elisa; Calì, Bianca; Bortoluzzi, Stefania; Bisognin, Andrea; Wang, Ena; Marincola, Francesco M; Naldini, Luigi; Gentner, Bernhard; Trautwein, Christian; Sackett, Sara Dutton; Zanovello, Paola; Molon, Barbara; Bronte, Vincenzo 2013-06-27 Tumor progression is accompanied by an altered myelopoiesis causing the accumulation of immunosuppressive cells. Here, we showed that miR-142-3p downregulation promoted macrophage differentiation and determined the acquisition of their immunosuppressive function in tumor. Tumor-released cytokines signaling through gp130, the common subunit of the interleukin-6 cytokine receptor family, induced the LAP∗ isoform of C/EBPβ transcription factor, promoting macrophage generation. miR-142-3p downregulated gp130 by canonical binding to its messenger RNA (mRNA) 3' UTR and repressed C/EBPβ LAP∗ by noncanonical binding to its 5' mRNA coding sequence. Enforced miR expression impaired macrophage differentiation both in vitro and in vivo. Mice constitutively expressing miR-142-3p in the bone marrow showed a marked increase in survival following immunotherapy with tumor-specific T lymphocytes. By modulating a specific miR in bone marrow precursors, we thus demonstrated the feasibility of altering tumor-induced macrophage differentiation as a potent tool to improve the efficacy of cancer immunotherapy. 2. Study of the Ne(^3P_2) + CH_3F Electron Transfer Reaction below 1 Kelvin CERN Document Server Jankunas, Justin; Osterwalder, Andreas 2014-01-01 Relatively little is known about the dynamics of electron transfer reactions at low collision energy. We present a study of Penning ionization of ground state methyl fluoride molecules by electronically excited neon atoms in the 13 \\mueV--4.8 meV (150 mK--56 K) collision energy range, using a neutral-neutral merged beam setup. Relative cross sections have been measured for three Ne(^3P_2)+ CH_3F reaction channels by counting the number of CH_3F^+, CH_2F^+, and CH_3^+ product ions, as a function of relative velocity between the neon and methyl fluoride molecular beams. Experimental cross sections markedly deviate from the Langevin capture model at collision energies above 20 K. The branching ratios are constant. In other words, the chemical shape of the CH_3F molecule, as seen by Ne(^3P_2) atom, appears not to change as the collision energy is varied, in contrast to related Ne(^3P_J) + CH_3X (X=Cl and Br) reactions at higher collision energies. 3. Cu(I)/Cu(II) templated functional pseudorotaxanes and rotaxanes Indian Academy of Sciences (India) Subrata Saha; Pradyut Ghosh 2012-11-01 Threaded complexes like pseudorotaxanes, rotaxanes based on Cu(I)/Cu(II) ions have shown to be promising for the construction of mechanically interlocked molecular-level architectures. In this short review, we focus on the synthetic strategies developed to construct pseudorotaxanes and rotaxanes using Cu(I)/Cu(II) ions as template. Further, brief discussions on chemical and mechanical properties associated with some of the selected to Cu(I)/Cu(II) based pseudorotaxanes and rotaxanes are presented. 4. miR-409-3p suppresses breast cancer cell growth and invasion by targeting Akt1 Energy Technology Data Exchange (ETDEWEB) Zhang, Guoqiang [Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan 250012 (China); Department of Thyroid and Breast Surgery, Hospital Affiliated to Binzhou Medical University, 661 Second Huanghe Street, Binzhou 256603 (China); Liu, Zengyan [Department of Hematology, Hospital Affiliated to Binzhou Medical University, 661 Second Huanghe Street, Binzhou 256603 (China); Xu, Hao [Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029 (China); Yang, Qifeng, E-mail: qifengy_sdu1@163.com [Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan 250012 (China) 2016-01-08 Altered levels and functions of microRNAs (miRNAs) are correlated with carcinogenesis. While miR-409-3p has been shown to play important roles in several cancer types, its function in the context of breast cancer (BC) remains unknown. In this study, miR-409-3p was significantly downregulated in BC tissues and cell lines, compared with the corresponding control counterparts. Overexpression of miR-409-3p inhibited BC cell proliferation, migration and invasion in vitro and suppressed tumor growth in vivo. Notably, miR-409-3p induced downregulation of Akt1 protein through binding to its 3′ untranslated region (UTR). Conversely, restoring Akt1 expression rescued the suppressive effects of miR-409-3p. Our data collectively indicate that miR-409-3p functions as a tumor suppressor in BC through downregulating Akt1, supporting the targeting of the novel miR-409-3p/Akt1 axis as a potentially effective therapeutic approach for BC. - Highlights: • miR-409-3p inhibits proliferation, migration and invasion of BC cells. • miR-409-3p suppresses tumor growth in nude mice. • Akt1 is a direct downstream target of miR-409-3p. • Ectopic expression of Akt1 reverses the effects of miR-409-3p on cell proliferation, migration and invasion. 5. Experimental Conditions: SE3_S01_M03_D03 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S01_M03_D03 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...1 Solanum lycopersicum Lovely ai fruit SE3_S01_M03 6.7mg [MassBase ID] MDLC1_25528 SE3_MS1 LC-FT-ICR-MS ESI 6. Experimental Conditions: SE3_S01_M03_D01 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S01_M03_D01 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...1 Solanum lycopersicum Lovely ai fruit SE3_S01_M03 6.7mg [MassBase ID] MDLC1_25528 SE3_MS1 LC-FT-ICR-MS ESI 7. Experimental Conditions: SE3_S01_M03_D02 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S01_M03_D02 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...1 Solanum lycopersicum Lovely ai fruit SE3_S01_M03 6.7mg [MassBase ID] MDLC1_25528 SE3_MS1 LC-FT-ICR-MS ESI 8. Experimental Conditions: SE3_S01_M02_D01 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S01_M02_D01 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...1 Solanum lycopersicum Lovely ai fruit SE3_S01_M02 6.7 mg [MassBase ID] MDLC1_25527 SE3_MS1 LC-FT-ICR-MS ESI 9. Experimental Conditions: SE3_S01_M01_D03 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S01_M01_D03 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...1 Solanum lycopersicum Lovely ai fruit SE3_S01_M01 6.7 mg [MassBase ID] MDLC1_25539 SE3_MS1 LC-FT-ICR-MS ESI 10. Experimental Conditions: SE3_S01_M01_D01 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S01_M01_D01 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...1 Solanum lycopersicum Lovely ai fruit SE3_S01_M01 6.7 mg [MassBase ID] MDLC1_25539 SE3_MS1 LC-FT-ICR-MS ESI 11. Experimental Conditions: SE3_S01_M02_D02 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S01_M02_D02 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...1 Solanum lycopersicum Lovely ai fruit SE3_S01_M02 6.7 mg [MassBase ID] MDLC1_25527 SE3_MS1 LC-FT-ICR-MS ESI 12. Experimental Conditions: SE3_S01_M02_D03 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S01_M02_D03 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...1 Solanum lycopersicum Lovely ai fruit SE3_S01_M02 6.7 mg [MassBase ID] MDLC1_25527 SE3_MS1 LC-FT-ICR-MS ESI 13. Experimental Conditions: SE3_S01_M01_D02 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S01_M01_D02 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...1 Solanum lycopersicum Lovely ai fruit SE3_S01_M01 6.7 mg [MassBase ID] MDLC1_25539 SE3_MS1 LC-FT-ICR-MS ESI 14. Experimental Conditions: SE3_S02_M03_D02 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S02_M03_D02 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...2 Solanum lycopersicum House Momotaro fruit SE3_S02_M03 6.7 mg [MassBase ID] MDLC1_25531 SE3_MS1 LC-FT-ICR-M 15. Experimental Conditions: SE3_S02_M03_D03 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S02_M03_D03 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...2 Solanum lycopersicum House Momotaro fruit SE3_S02_M03 6.7 mg [MassBase ID] MDLC1_25531 SE3_MS1 LC-FT-ICR-M 16. Experimental Conditions: SE3_S02_M02_D03 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S02_M02_D03 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...2 Solanum lycopersicum House Momotaro fruit SE3_S02_M02 6.7 mg [MassBase ID] MDLC1_25530 SE3_MS1 LC-FT-ICR-M 17. Experimental Conditions: SE3_S02_M03_D01 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S02_M03_D01 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...2 Solanum lycopersicum House Momotaro fruit SE3_S02_M03 6.7 mg [MassBase ID] MDLC1_25531 SE3_MS1 LC-FT-ICR-M 18. Experimental Conditions: SE3_S02_M01_D02 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S02_M01_D02 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...2 Solanum lycopersicum House Momotaro fruit SE3_S02_M01 6.7mg [MassBase ID] MDLC1_25529 SE3_MS1 LC-FT-ICR-MS 19. Experimental Conditions: SE3_S02_M02_D01 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S02_M02_D01 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...2 Solanum lycopersicum House Momotaro fruit SE3_S02_M02 6.7 mg [MassBase ID] MDLC1_25530 SE3_MS1 LC-FT-ICR-M 20. Experimental Conditions: SE3_S02_M01_D03 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S02_M01_D03 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...2 Solanum lycopersicum House Momotaro fruit SE3_S02_M01 6.7mg [MassBase ID] MDLC1_25529 SE3_MS1 LC-FT-ICR-MS 1. Experimental Conditions: SE3_S02_M02_D02 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S02_M02_D02 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...2 Solanum lycopersicum House Momotaro fruit SE3_S02_M02 6.7 mg [MassBase ID] MDLC1_25530 SE3_MS1 LC-FT-ICR-M 2. Experimental Conditions: SE3_S02_M01_D01 [Metabolonote[Archive Lifescience Database Archive (English) Full Text Available SE3_S02_M01_D01 SE3 Comparison of fruit metabolites among tomato varieties 1 SE3_S0...2 Solanum lycopersicum House Momotaro fruit SE3_S02_M01 6.7mg [MassBase ID] MDLC1_25529 SE3_MS1 LC-FT-ICR-MS 3. miR-342-3p affects hepatocellular carcinoma cell proliferation via regulating NF-κB pathway Energy Technology Data Exchange (ETDEWEB) Zhao, Liang; Zhang, Yubao, E-mail: zhyb880077@sina.com 2015-02-13 Recent research indicates that non-coding microRNAs (miRNAs) help regulate basic cellular processes in many types of cancer cells. We hypothesized that overexpression of miR-342-3p might affect proliferation of hepatocellular carcinoma (HCC) cells. After confirming overexpression of miR-342-3p with qRT-PCR, MTT assay showed that HCC cell proliferation was significantly inhibited by miR-342-3p, and that it significantly decreased BrdU-positive cell proliferation by nearly sixfold. Searching for targets using three algorithms we found that miR-342-3p is related to the NF-κB pathway and luciferase assay found that IKK-γ, TAB2 and TAB3 are miR-342-3p target genes. Results of western blot on extracted nuclear proteins of HepG2 and HCT-116 cells showed that miR-342-3p reduced and miR-342-3p-in increased p65 nuclear levels and qRT-PCR found that NF-κB pathway downstream genes were downregulated by miR-342-3p and upregulated by miR-342-3p-in, confirming that miR-342 targets NF-κB pathway. Overexpression of Ikk-γ, TAB2 and TAB3 partially rescued HCC cells proliferation inhibited by miR-342-3p. Using the GSE54751 database we evaluated expression from 10 HCC samples, which strongly suggested downregulation of miR-342-3p and we also found inverse expression between miR-342-3p and its targets IKK-γ, TAB2 and TAB3 from 71 HCC samples. Our results show that miR-342-3p has a significant role in HCC cell proliferation and is suitable for investigation of therapeutic targets. - Highlights: • MiR-342-3p suppresses hepatocellular carcinoma cell proliferation. • MiR-342-3p targets IKK-γ, TAB2 and TAB3 genes. • MiR-342-3p downregulates NF-kB signaling pathway. • MiR-342-3p is downregulated in clinical hepatocellular carcinoma samples. • The expression of miR-342-3p and its target gene is inversely related. 4. MiR-490-3p sensitizes ovarian cancer cells to cisplatin by directly targeting ABCC2 Science.gov (United States) Tian, Jing; Xu, Yan-Ying; Li, Lian; Hao, Quan 2017-01-01 Cisplatin (CDDP) resistance becomes a large obstacle of the beneficial therapy for patients with ovarian cancer. MicroRNAs (miRNAs) act as post-transcriptional regulators of multiple genes’ expression and have been reported to be involved in multi-drug resistance. The purpose of this study was to determine the roles and molecular mechanism of miR-490-3p in the CDDP resistance in ovarian cancer. We found that miR-490-3p was downregulated in CDDP-resistant OVCAR3/CDDP and SKOV3/CDDP cells, which was due to the hypermethylation of miR-490-3p promoter. Functional studies demonstrated that miR-490-3p increased the cell response to CDDP in OVCAR3, SKOV3 and CDDP-resistant cells, while miR-490-3p inhibition resulted in opposite effects. Luciferase assay, real-time PCR and Western blot as well as immunohistochemistry validated that ABCC2 was a direct target of miR-490-3p and miR-490-3p downregulated ABCC2 expression via binding to its 3’UTR. Importantly, silencing of ABCC2 alleviated CDDP resistance induced by miR-490-3p inhibition, while ABCC2 overexpression restored CDDP resistance inhibited by miR-490-3p. In vivo study showed that miR-490-3p enhanced the cytotoxicity of CDDP. Finally, we found that miR-490-3p was downregulated in CDDP-resistant ovarian cancer tissues, while ABCC2 was upregulated. In conclusion, our data indicate that miR-490-3p enhances CDDP sensitivity of ovarian cancer cells through downregulating ABCC2 expression, and suggest that delivery of miR-490-3p might be a potential therapeutic strategy for patients with CDP-resistant ovarian cancer. 5. MicroRNA-127-3p inhibits proliferation and invasion by targeting SETD8 in human osteosarcoma cells Energy Technology Data Exchange (ETDEWEB) Zhang, Jun; Hou, Wengen; Chai, Mingxiang; Zhao, Hongxing; Jia, Jinling; Sun, Xiaohui; Zhao, Bin [Second Department of Orthopaedics, The First Affiliated Hospital of XinXiang Medical College, XinXiang 453100 (China); Wang, Ran, E-mail: wangranfire@sohu.com [Department of Pediatrics, The First Affiliated Hospital, ZhengZhou University, ZhengZhou 450000 (China) 2016-01-22 MicroRNAs (miRNAs) play an essential role in cancer development. Several studies have indicated that miRNAs mediate tumorigenesis processes, such as, inflammation, proliferation, apoptosis and invasion. In the present study, we focused on the influence of the miR-127-3p on the proliferation, migration and invasion of osteosarcoma (OS). MiR-127-3p was found at reduced levels in OS tissues and cell lines. Overexpression of miR-127-3p in the OS cell lines significantly inhibited the cell proliferation, migration and invasion; however, inhibition of miR-127-3p increased the proliferation, migration and invasion of OS in vitro. SETD8 was identified as a direct target of miR-127-3p, and SETD8 expression decreased post miR-127-3p overexpression, while SETD8 overexpression could reverse the potential influence of miR-127-3p on the migration and invasion of OS cells. MiR-127-3p is suggested to act mainly via the suppression of SETD8 expression. Overall, the results revealed that miR-127-3p acts as a tumor suppressor and that its down-regulation in cancer may contribute to OS progression and metastasis, suggesting that miR-127-3p could be a potential therapeutic target in the treatment of OS. - Highlights: • MiR-127-3p is decreased in osteosarcoma tissues and cell lines. • MiR-127-3p overexpression suppresses cell migration and invasion in MG63 and U2OS. • SETD8 overexpression abolishes the roles of miR-127-3p in osteosarcoma. 6. Laser Flash Photolysis Studies of Radical-Radical Reaction Kinetics: The O((sup 3)P(sub J)) + BrO Reaction Science.gov (United States) Thorn, R. P.; Cronkhite, J. M.; Nicovich, J. M.; Wine, P. H. 1997-01-01 A novel dual laser flash photolysis-long path absorption-resonance fluorescence technique has been employed to study the kinetics of the important stratospheric reaction 0((sup 3)P(sub j)) + Br yields(k1) BrO((sup 2)P(sub J)) + O2 as a function of temperature (231-328 K) and pressure (25-150 Torr) in N2 buffer gas. The experimental approach preserves the principal advantages of the flash photolysis method, i.e., complete absence of surface reactions and a wide range of accessible pressures, but also employs techniques which are characteristic of the discharge flow method, namely chemical titration as a means for deducing the absolute concentration of a radical reactant and use of multiple detection axes. We find that k1 is independent of pressure, and that the temperature dependence of k1 is adequately described by the Arrhenius expression k1(T) = 1.91 x 10(exp -11)(230/J) cu cm/ molecule.s; the absolute accuracy of measured values for k1 is estimated to vary from +/- 20 percent at at T approximately 230 K to +/- 30 percent at T approximately 330 K. Our results demonstrate that the O((sup 3)P(sub j)) + BrO rate coefficient is significantly faster than previously 'guesstimated,' and suggest that the catalytic cycle with the O((sup 3)P(sub j)) + BrO reaction as its rate-limiting step is the dominant stratospheric BrO(x), odd-oxygen destruction cycle at altitudes above 24 km. 7. Absolute frequency measurement of the 1S0 - 3P0 transition of 171Yb CERN Document Server Pizzocaro, Marco; Rauf, Benjamin; Bregolin, Filippo; Milani, Gianmaria; Clivati, Cecilia; Costanzo, Giovanni A; Levi, Filippo; Calonico, Davide 2016-01-01 We report the absolute frequency measurement of the unperturbed transition 1S0 - 3P0 at 578 nm in 171Yb realized in an optical lattice frequency standard. The absolute frequency is measured 518 295 836 590 863.55(28) Hz relative to a cryogenic caesium fountain with a fractional uncertainty of 5.4x10-16 . This value is in agreement with the ytterbium frequency recommended as a secondary representation of the second in the International System of Units. 8. Pulsar Spin-Down by 3P2 Superfluid Neutron with Field Decay Institute of Scientific and Technical Information of China (English) LUO Xin-Lian; PENG Qiu-He; CHOU Chih-Kang 2003-01-01 To describe pulsar spin-down, we present a simple combined torque model that takes into account both the standard magnetic dipole radiation and the electromagnetic radiation from the 3P2 superSuid vortex neutrons inside neutron star. Using an ordinary exponential model for the magnetic field decay, we derive an analytical formulae for pulsar evolution tracks. The pulsar evolution on the P-P diagram is quite different from that of the standard magnetic dipole radiation model, especially when the supernuid torque or Geld decay becomes dominant. 9. The dielectronic satellites to the 2s-3p Ne-like krypton resonance lines Energy Technology Data Exchange (ETDEWEB) Khakhalin, S.Ya. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Dyakin, V.M. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Faenov, A.Ya. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Fiedorowicz, H. (Inst. of Optoelectronics, Warsaw (Poland)); Bartnik, A. (Inst. of Optoelectronics, Warsaw (Poland)); Parys, P. (Inst. of Plasma Physics and Laser Microfusion, Warsaw (Poland)); Nilsen, J. (Lawrence Livermore National Lab., Livermore, CA (United States)); Osterheld, A. (Lawrence Livermore National Lab., Livermore, CA (United States)) 1994-08-01 We present an analysis of dielectronic satellite spectra of 2p[sup 6]-2s2p[sup 6]3p Ne-like krypton resonance lines. The satellite structure was registered with high (better than [lambda]/[Delta][lambda] > 3500) spectral resolution in the emission of a laser irradiated gas puff target. We perform an unambiguous identification of satellite lines caused by radiative transitions from autoionizing states of sodium-like krypton ions. A total of about 20 spectral features are identified, most of them for the first time. Very good agreement between the satellite structure calculations and experimental emission spectra is obtained. (orig.). 10. Phase transitions in Cd3P2 at high pressures and high temperatures DEFF Research Database (Denmark) Yel'kin, F.S.; Sidorov, V.A.; Waskowska, A.; 2008-01-01 The high-pressure, high-temperature structural behaviour of Cd3P2 has been studied using electrical resistance measurements, differential thermal analysis, thermo baric analysis and X-ray diffraction. At room temperature, a phase transformation is observed at 4.0 GPa in compression....... The experimental zero-pressure bulk modulus of the low-pressure phase is 64.7(7) GPa, which agrees quite well with the calculated value of 66.3 GPa using the tight-binding linear muffin-tin orbital method within the local density approximation. Tentatively, the high-pressure phase has an orthorhombic crystal... 11. 75 FR 23574 - Airworthiness Directives; CFM International, S.A. CFM56-5B1/P, -5B2/P, -5B3/P, -5B3/P1, -5B4/P... Science.gov (United States) 2010-05-04 ..., S.A. CFM56-5B1/P, - 5B2/P, -5B3/P, -5B3/P1, -5B4/P, -5B5/P, -5B6/P, -5B7/P, -5B8/P, -5B9/P, -5B1/2P... Docket Operations office between 9 a.m. and 5 p.m., Monday through Friday, except Federal holidays. The... CFM International, S.A. CFM56-5B1/P, - 5B2/P, -5B3/P, -5B3/P1, -5B4/P, -5B5/P,...... 12. Search For the Lepton-Flavor Violating Decays Y(3S)->e tau and Y(3S)->mu tau Energy Technology Data Exchange (ETDEWEB) Aubert, B. 2008-12-11 Charged lepton-flavor violating processes are extremely rare in the Standard Model, but they are predicted to occur in several beyond-the-Standard Model theories, including Supersymmetry or models with leptoquarks or compositeness. We present a search for such processes in a sample of 117 x 10{sup 6} {Upsilon}(3S) decays recorded with the BABAR detector. We place upper limits on the branching fractions BF({Upsilon}(3S) {yields} e{sup {+-}}{tau}{sup {-+}}) < 5.0 x 10{sup -6} and BF({Upsilon}(3S) {yields} {mu}{sup {+-}}{tau}{sup {-+}}) < 4.1 x 10{sup -6} at 90% confidence level. These results are used to place lower limits on the mass scale of beyond-the-Standard Model physics contributing to lepton-flavor violating decays of the {Upsilon}(3S). 13. Study of Y(3S, 2S)-> eta Y(1S) and Y(3S, 2S) -> pi pi- Y(1S) Hadronic Transitions Energy Technology Data Exchange (ETDEWEB) Lees, J.P.; Poireau, V.; Tisserand, V.; /Annecy, LAPP; Garra Tico, J.; Grauges, E.; /Barcelona U., ECM; Martinelli, M.; /INFN, Bari /Bari U.; Milanes, D.A.; /INFN, Bari; Palano, A.; Pappagallo, M.; /INFN, Bari /Bari U.; Eigen, G.; Stugu, B.; /Bergen U.; Brown, D.N.; Kerth, L.T.; Kolomensky, Yu.G.; Lynch, G.; /UC, Berkeley; Koch, H.; Schroeder, T.; /Ruhr U., Bochum; Asgeirsson, D.J.; Hearty, C.; Mattison, T.S.; McKenna, J.A.; /British Columbia U. /Brunel U. /Novosibirsk, IYF /UC, Irvine /UC, Riverside /UC, Santa Barbara /UC, Santa Cruz /Caltech /Cincinnati U. /Colorado U. /Colorado State U. /Dortmund U. /Dresden, Tech. U. /Ecole Polytechnique /Edinburgh U. /INFN, Ferrara /INFN, Ferrara /Ferrara U. /INFN, Ferrara /Frascati /INFN, Genoa /Genoa U. /INFN, Genoa /INFN, Genoa /Genoa U. /INFN, Genoa /Indian Inst. Tech., Guwahati /Harvard U. /Harvey Mudd Coll. /Heidelberg U. /Humboldt U., Berlin /Imperial Coll., London /Iowa State U. /Iowa State U. /Johns Hopkins U. /Paris U., VI-VII /LLNL, Livermore /Liverpool U. /Queen Mary, U. of London /Royal Holloway, U. of London /Royal Holloway, U. of London /Louisville U. /Mainz U., Inst. Kernphys. /Manchester U. /Maryland U. /Massachusetts U., Amherst /MIT /McGill U. /INFN, Milan /Milan U. /INFN, Milan /INFN, Milan /Milan U. /Mississippi U. /Montreal U. /INFN, Naples /Naples U. /NIKHEF, Amsterdam /NIKHEF, Amsterdam /Notre Dame U. /Ohio State U. /Oregon U. /INFN, Padua /Padua U. /INFN, Padua /INFN, Padua /Padua U. /Paris U., VI-VII /INFN, Perugia /Perugia U. /INFN, Pisa /Pisa U. /INFN, Pisa /Pisa, Scuola Normale Superiore /INFN, Pisa /Pisa U. /INFN, Pisa /INFN, Pisa /Pisa U. /INFN, Pisa /Princeton U. /INFN, Rome /INFN, Rome /Rome U. /INFN, Rome /INFN, Rome /Rome U. /INFN, Rome /Rostock U. /Rutherford /DAPNIA, Saclay /SLAC /South Carolina U. /Southern Methodist U. /Stanford U., Phys. Dept. /SUNY, Albany /Tel Aviv U. /Tennessee U. /Texas Nuclear Corp., Austin /Texas U., Dallas /INFN, Turin /Turin U. /INFN, Trieste /Trieste U. /Valencia U. /Victoria U. /Warwick U. /Wisconsin U., Madison 2012-03-27 We study the {Upsilon}(3S, 2S) {yields} {eta}{Upsilon}(1S) and {Upsilon}(3S, 2S) {yields} {pi}{sup +}{pi}{sup -}{Upsilon}(1S) transitions with 122 x 10{sup 6} {Upsilon}(3S) and 100 x 10{sup 6} {Upsilon}(2S) mesons collected by the BABAR detector at the PEP-II asymmetric-energy e{sup +}e{sup -} collider. We measure {Beta}[{Upsilon}(2S) {yields} {eta}{Upsilon}(1S)] = (2.39 {+-} 0.31(stat.) {+-} 0.14(syst.)) x 10{sup -4} and {Lambda}[{Upsilon}(2S) {yields} {eta}{Upsilon}(1S)]/{Lambda}[{Upsilon}(2S) {yields} {pi}{sup +}{pi}{sup -}{Upsilon}(1S)] = (1.35 {+-} 0.17(stat.) {+-} 0.08(syst.)) x 10{sup -3}. We find no evidence for {Upsilon}(3S) {yields} {eta}{Upsilon}(1S) and obtain {Beta}[{Upsilon}(3S) {yields} {eta}{Upsilon}(1S)] < 1.0 x 10{sup -4} and {Lambda}[{Upsilon}(3S) {yields} {eta}{Upsilon}(1S)]/{Lambda}[{Upsilon}(3S) {yields} {pi}{sup +}{pi}{sup -}{Upsilon}(1S)] < 2.3 x 10{sup -3} as upper limits at the 90% confidence level. We also provide improved measurements of the {Upsilon}(2S)-{Upsilon}(1S) and {Upsilon}(3S)-{Upsilon}(1S) mass differences, 562.170 {+-} 0.007(stat.) {+-} 0.088(syst.)MeV/c{sup 2} and 893.813 {+-} 0.015(stat.) {+-} 0.107(syst.)MeV/c{sup 2}, respectively. 14. Study of Y(3S, 2S)-> eta Y(1S) and Y(3S, 2S) -> pi pi- Y(1S) Hadronic Transitions Energy Technology Data Exchange (ETDEWEB) Lees, J.P.; Poireau, V.; Tisserand, V.; /Annecy, LAPP; Garra Tico, J.; Grauges, E.; /Barcelona U., ECM; Martinelli, M.; /INFN, Bari /Bari U.; Milanes, D.A.; /INFN, Bari; Palano, A.; Pappagallo, M.; /INFN, Bari /Bari U.; Eigen, G.; Stugu, B.; /Bergen U.; Brown, D.N.; Kerth, L.T.; Kolomensky, Yu.G.; Lynch, G.; /UC, Berkeley; Koch, H.; Schroeder, T.; /Ruhr U., Bochum; Asgeirsson, D.J.; Hearty, C.; Mattison, T.S.; McKenna, J.A.; /British Columbia U. /Brunel U. /Novosibirsk, IYF /UC, Irvine /UC, Riverside /UC, Santa Barbara /UC, Santa Cruz /Caltech /Cincinnati U. /Colorado U. /Colorado State U. /Dortmund U. /Dresden, Tech. U. /Ecole Polytechnique /Edinburgh U. /INFN, Ferrara /INFN, Ferrara /Ferrara U. /INFN, Ferrara /Frascati /INFN, Genoa /Genoa U. /INFN, Genoa /INFN, Genoa /Genoa U. /INFN, Genoa /Indian Inst. Tech., Guwahati /Harvard U. /Harvey Mudd Coll. /Heidelberg U. /Humboldt U., Berlin /Imperial Coll., London /Iowa State U. /Iowa State U. /Johns Hopkins U. /Paris U., VI-VII /LLNL, Livermore /Liverpool U. /Queen Mary, U. of London /Royal Holloway, U. of London /Royal Holloway, U. of London /Louisville U. /Mainz U., Inst. Kernphys. /Manchester U. /Maryland U. /Massachusetts U., Amherst /MIT /McGill U. /INFN, Milan /Milan U. /INFN, Milan /INFN, Milan /Milan U. /Mississippi U. /Montreal U. /INFN, Naples /Naples U. /NIKHEF, Amsterdam /NIKHEF, Amsterdam /Notre Dame U. /Ohio State U. /Oregon U. /INFN, Padua /Padua U. /INFN, Padua /INFN, Padua /Padua U. /Paris U., VI-VII /INFN, Perugia /Perugia U. /INFN, Pisa /Pisa U. /INFN, Pisa /Pisa, Scuola Normale Superiore /INFN, Pisa /Pisa U. /INFN, Pisa /INFN, Pisa /Pisa U. /INFN, Pisa /Princeton U. /INFN, Rome /INFN, Rome /Rome U. /INFN, Rome /INFN, Rome /Rome U. /INFN, Rome /Rostock U. /Rutherford /DAPNIA, Saclay /SLAC /South Carolina U. /Southern Methodist U. /Stanford U., Phys. Dept. /SUNY, Albany /Tel Aviv U. /Tennessee U. /Texas Nuclear Corp., Austin /Texas U., Dallas /INFN, Turin /Turin U. /INFN, Trieste /Trieste U. /Valencia U. /Victoria U. /Warwick U. /Wisconsin U., Madison 2012-03-27 We study the {Upsilon}(3S, 2S) {yields} {eta}{Upsilon}(1S) and {Upsilon}(3S, 2S) {yields} {pi}{sup +}{pi}{sup -}{Upsilon}(1S) transitions with 122 x 10{sup 6} {Upsilon}(3S) and 100 x 10{sup 6} {Upsilon}(2S) mesons collected by the BABAR detector at the PEP-II asymmetric-energy e{sup +}e{sup -} collider. We measure {Beta}[{Upsilon}(2S) {yields} {eta}{Upsilon}(1S)] = (2.39 {+-} 0.31(stat.) {+-} 0.14(syst.)) x 10{sup -4} and {Lambda}[{Upsilon}(2S) {yields} {eta}{Upsilon}(1S)]/{Lambda}[{Upsilon}(2S) {yields} {pi}{sup +}{pi}{sup -}{Upsilon}(1S)] = (1.35 {+-} 0.17(stat.) {+-} 0.08(syst.)) x 10{sup -3}. We find no evidence for {Upsilon}(3S) {yields} {eta}{Upsilon}(1S) and obtain {Beta}[{Upsilon}(3S) {yields} {eta}{Upsilon}(1S)] < 1.0 x 10{sup -4} and {Lambda}[{Upsilon}(3S) {yields} {eta}{Upsilon}(1S)]/{Lambda}[{Upsilon}(3S) {yields} {pi}{sup +}{pi}{sup -}{Upsilon}(1S)] < 2.3 x 10{sup -3} as upper limits at the 90% confidence level. We also provide improved measurements of the {Upsilon}(2S)-{Upsilon}(1S) and {Upsilon}(3S)-{Upsilon}(1S) mass differences, 562.170 {+-} 0.007(stat.) {+-} 0.088(syst.)MeV/c{sup 2} and 893.813 {+-} 0.015(stat.) {+-} 0.107(syst.)MeV/c{sup 2}, respectively. 15. The reactions of O({sup 3}P) with alkenes: The formyl radical channel Energy Technology Data Exchange (ETDEWEB) Min, Z.; Wong, T.H.; Quandt, R.; Bersohn, R. 1999-12-09 The HCO product of the reaction of O({sup 3}P) with ethene has been detected by cavity ring-down spectroscopy using its A-X transition. For propene a somewhat smaller yield of HCO was obtained but the overall rate constant is much larger. The yield of HCO in this reaction is quite small ({approximately}0.05). Moreover, a large number of other alkenes were tried with negative results. The failure of the 1,2 H atom shift followed by breaking the 1,2 bond implies that the unimolecular decomposition has found a more favorable channel. The proposed mechanism is as follows. For an alkene of the form RCH{sub 2}{double{underscore}bond}CH{sub 2} the first step is attachment of the O({sup 3}P) to the terminal carbon atom, C{sub 1}. Then, intersystem crossing occurs and finally a H atom shifts from C{sub 3} to C{sub 2} and not from C{sub 1} to C{sub 2}. In this way a molecule of formaldehyde and an alkene shorter by one carbon atom are formed. 16. Metalloid tolerance based on phytochelatins is not functionally equivalent to the arsenite transporter Acr3p. Science.gov (United States) Wysocki, Robert; Clemens, Stephan; Augustyniak, Daria; Golik, Pawel; Maciaszczyk, Ewa; Tamás, Markus J; Dziadkowiec, Dorota 2003-05-01 Active transport of metalloids by Acr3p and Ycf1p in Saccharomyces cerevisiae and chelation by phytochelatins in Schizosaccharomyces pombe, nematodes, and plants represent distinct strategies of metalloid detoxification. In this report, we present results of functional comparison of both resistance mechanisms. The S. pombe and wheat phytochelatin synthase (PCS) genes, when expressed in S. cerevisiae, mediate only modest resistance to arsenite and thus cannot functionally compensate for Acr3p. On the other hand, we show for the first time that phytochelatins also contribute to antimony tolerance as PCS fully complement antimonite sensitivity of ycf1Delta mutant. Remarkably, heterologous expression of PCS sensitizes S. cerevisiae to arsenate, while ACR3 confers much higher arsenic resistance in pcsDelta than in wild-type S. pombe. The analysis of PCS and ACR3 homologues distribution in various organisms and our experimental data suggest that separation of ACR3 and PCS genes may lead to the optimal tolerance status of the cell. 17. The strength and microstructure of Cu joints brazed with Cu-P based amorphous brazing filler metal contained B%含硼Cu-P基非晶钎料钎焊紫铜接头的连接强度及微观组织 Institute of Scientific and Technical Information of China (English) 邹家生; 李华其; 王超 2012-01-01 The copper joints were brazed with CuP7. 7Sn5.4Ni14Si0. 2B0. 03amorphous filler metal and conventional filler metal, the microstructure of filler metal and brazing joint were analyzed and the effect of brazing procedure on joints strength was studied. The results show that the brazing procedure has great effects on the brazing joints of both of the CuP7. 7Sn5.4Ni14Si0. 2B0. 03 normal and amorphous filler metal. In the same experimental condition, the temperature has more effects on the shear strength. And the shear strength of amorphous metal joints are always higher than the normal. The microstructure of diffusion and interface zone in joint brazed with CuP7. 7Sn5. 4Ni14Si0. 2B0. 03 amorphous brazing filler metal are mainly composed of a - Cu solid solution, and the center in the brazing seam is mainly composed of a - Cu and ( Cu, Ni) 3 P and (α - Cu + Cu3P) eutectic and (a - Cu + Cu3P + 8) ternary eutectic. A large number of Cu -P, Ni -P, Cu -Sn and other brittle phases exist on the fracture interface of amorphous CuF7. 7Sn5. 4Ni14Si0. 2B0. 03 filler metal joint, therefore, reducing the number of fragile compounds will help to increase the shear strength of the joints.%采用CuP7.7Sn5.4Ni14Si0.2B0.03非晶和常规钎料钎焊紫铜接头,研究了钎焊工艺对连接强度的影响,分析了钎焊接头的微观组织.研究结果表明:钎焊工艺对CuP7.7Sn5.4Ni14Si0.2B0.03常规与非晶钎料钎焊接头的强度均有明显影响,同样条件下,钎焊温度的影响更大；非晶钎料钎焊接头的剪切强度始终高于常规钎料；CuP7.7Sn5.4Ni14Si0.2B0.03非晶钎料钎焊接头扩散区和界面区主要是α - Cu固溶体组织,钎缝中心区主要为α-Cu+ (Cu,Ni)3P+(α- Cu+ Cu3P)共晶+(α-Cu+Cu3P+δ)共晶的混合组织；CuP7.7Sn5.4Ni14Si0.2B0.03钎焊接头断裂界面处存在大量的Cu -P,Ni -P,Cu - Sn等脆性相,因此,减少脆性化合物的数量有助于提高钎焊接头强度. 18. MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1. Science.gov (United States) Wu, Shifeng; Du, Xinjie; Wu, Manwu; Du, Hechun; Shi, Xiaoliang; Zhang, Tao 2016-06-10 Osteosarcoma is the most common primary bone cancer which is associated with early metastatic potential and poor prognosis. However, the molecular mechanisms underlying osteosarcoma progression are not well characterized. Here, we investigated the role of miR-409-3p in osteosarcoma metastasis. Osteosarcoma tissue showed decreased expression of miR-409-3p compared to adjacent non-tumorous tissue. The expression level of miR-409-3p was negatively correlated with osteosarcoma metastasis. Overexpression of miR-409-3p in osteosarcoma cells (U2OS) inhibited cell migration and invasion. Bioinformatics analysis showed that catenin-δ1 (CTNND1, p120-catenin) is a direct target of miR-409-3p. Overexpression of miR-409-3p repressed the expression of catenin-δ1 in U2OS cells at both mRNA and protein levels. Meanwhile, miR-409-3p repressed the activity of luciferase reporter containing the 3'-untranslated region (3'UTR) of CTNND1 gene. Furthermore, expression of catenin-δ1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion. Altogether, these results indicated that miR-409-3p targets catenin-δ1 to repress osteosarcoma metastasis. 19. Phase equilibria in the Mo-Fe-P system at 800 °C and structure of ternary phosphide (Mo(1-x)Fe(x))3P (0.10 ≤ x ≤ 0.15). Science.gov (United States) Oliynyk, Anton O; Lomnytska, Yaroslava F; Dzevenko, Mariya V; Stoyko, Stanislav S; Mar, Arthur 2013-01-18 Construction of the isothermal section in the metal-rich portion (<67 atom % P) of the Mo-Fe-P phase diagram at 800 °C has led to the identification of two new ternary phases: (Mo(1-x)Fe(x))(2)P (x = 0.30-0.82) and (Mo(1-x)Fe(x))(3)P (x = 0.10-0.15). The occurrence of a Co(2)Si-type ternary phase (Mo(1-x)Fe(x))(2)P, which straddles the equiatomic composition MoFeP, is common to other ternary transition-metal phosphide systems. However, the ternary phase (Mo(1-x)Fe(x))(3)P is unusual because it is distinct from the binary phase Mo(3)P, notwithstanding their similar compositions and structures. The relationship has been clarified through single-crystal X-ray diffraction studies on Mo(3)P (α-V(3)S-type, space group I42m, a = 9.7925(11) Å, c = 4.8246(6) Å) and (Mo(0.85)Fe(0.15))(3)P (Ni(3)P-type, space group I4, a = 9.6982(8) Å, c = 4.7590(4) Å) at -100 °C. Representation in terms of nets containing fused triangles provides a pathway to transform these closely related structures through twisting. Band structure calculations support the adoption of these structure types and the site preference of Fe atoms. Electrical resistivity measurements on (Mo(0.85)Fe(0.15))(3)P reveal metallic behavior but no superconducting transition. 20. Unified interpretation of Hund's first and second rules for 2p and 3p atoms. Science.gov (United States) Oyamada, Takayuki; Hongo, Kenta; Kawazoe, Yoshiyuki; Yasuhara, Hiroshi 2010-10-28 A unified interpretation of Hund's first and second rules for 2p (C, N, O) and 3p (Si, P, S) atoms is given by Hartree-Fock (HF) and multiconfiguration Hartree-Fock (MCHF) methods. Both methods exactly satisfy the virial theorem, in principle, which enables one to analyze individual components of the total energy E(=T+V(en)+V(ee)), where T, V(en), and V(ee) are the kinetic, the electron-nucleus attraction, and the electron-electron repulsion energies, respectively. The correct interpretation for each of the two rules can only be achieved under the condition of the virial theorem 2T+V=0 by investigating how V(en) and V(ee) interplay to attain the lower total potential energy V(=V(en)+V(ee)). The stabilization of the more stable states for all the 2p and 3p atoms is ascribed to a greater V(en) that is caused by contraction of the valence orbitals accompanied with slight expansion of the core orbitals. The contraction of the valence orbitals for the two rules is a consequence of reducing the Hartree screening of the nucleus at short interelectronic distances. The reduced screening in the first rule is due to a greater amount of Fermi hole contributions in the state with the highest total spin-angular momentum S. The reduced screening in the second rule is due to the fact that two valence electrons are more likely to be on opposite sides of the nucleus in the state with the highest total orbital-angular momentum L. For each of the two rules, the inclusion of correlation does not qualitatively change the HF interpretation, but HF overestimates the energy difference ∣ΔE∣ between two levels being compared. The magnitude of the correlation energy is significantly larger for the lower L states than for the higher L states since two valence electrons in the lower L states are less likely to be on opposite sides of the nucleus. The MCHF evaluation of ∣ΔE∣ is in excellent agreement with experiment. The present HF and MCHF calculations demonstrate the above statements 1. Influence of Nucleating Agent on the Formation of C4A3S Institute of Scientific and Technical Information of China (English) GUO Huiling; XIE Junlin; ZHANG Shu; XU Qi 2012-01-01 The reference test methods are carried out parallelly,by means of chemical analysis,X-ray diffraction,differential scanning calorimetry-thermogravimetry,scanning electron microscopy and polarized optical microscope to study the formation of C4A3S in the presence and absence of nucleating agent.The results show that nucleating agent with high calcium and low heat consumption as tricalcium silicate (C3S) promotes the formation of C4A3S and increases desulfurization degree obviously.During calcining raw meals doped with C3S,the grain sizes of C4A3S are larger compared with that without C3S.And at the same calcining level,the mass loss and the heat consumption belonged to CaCO3 decomposition is reduced. 2. MiR-519d-3p suppresses invasion and migration of trophoblast cells via targeting MMP-2. Directory of Open Access Journals (Sweden) Jie Ding Full Text Available Our study was approved by the Medical Ethics Committee of Tang Du Hospital, Fourth Military Medical University and complied strictly with national ethical guidelines. Preeclampsia (PE is a specific clinical disorder characterized by gestational hypertension and proteinuria and is a leading cause of maternal and perinatal mortality worldwide. The miR-519d-3p is upregulated in the maternal plasma of patients with PE which indicates a possible association between this microRNA and the pathogenesis of PE. No studies to date have addressed the effect of miR-519d-3p on the invasion and migration of trophoblast cells. In our study, we found that miR-519d-3p expression was elevated in placental samples from patients with PE. In vitro, overexpression of miR-519d-3p significantly inhibited trophoblast cell migration and invasion, whereas transfection of a miR-519d-3p inhibitor enhanced trophoblast cell migration and invasion. Luciferase assays confirmed that matrix metalloproteinase-2 (MMP-2 is a direct target of miR-519d-3p. Quantitative real-time PCR and western blot assays showed that overexpression of miR-519d-3p downregulated MMP-2 mRNA and protein expression. Knockdown of MMP-2 using a siRNA attenuated the increased trophoblast migration and invasion promoted by the miR-519d-3p inhibitor. In placentas from patients with PE or normal pregnancies, a negative correlation between the expression of MMP-2 and miR-519d-3p was observed using the Pearson correlation and linear regression analysis. Our present findings suggest that upregulation of miR-519d-3p may contribute to the development of PE by inhibiting trophoblast cell migration and invasion via targeting MMP-2; miR-519d-3p may represent a potential predictive and therapeutic target for PE. 3. MicroRNA-27a-3p Inhibits Melanogenesis in Mouse Skin Melanocytes by Targeting Wnt3a. Science.gov (United States) Zhao, Yuanyuan; Wang, Pengchao; Meng, Jinzhu; Ji, Yuankai; Xu, Dongmei; Chen, Tianzhi; Fan, Ruiwen; Yu, Xiuju; Yao, Jianbo; Dong, Changsheng 2015-05-14 MicroRNAs (miRNAs) play an essential role in the regulation of almost all the biological processes, including melanogenesis. MiR-27a-3p is nearly six times higher in white alpaca skin compared to brown skin, which indicates that miR-27a-3p may be a candidate regulator for melanogenesis. Wnt3a plays an important role in promoting melanoblasts to differentiate into melanocytes and melanogenesis. To confirm the function of miR-27a-3p to melanogenesis in mammals, miR-27a-3p mimic, inhibitor and their negative control were transfected into mouse melanocytes. As a result, miR-27a-3p inhibits melanogenesis by repressing Wnt3a at post-transcriptional level. A significant decrease in Wnt3a luciferase activity was observed in 293T cells co-transfected with the matched luciferase reporter vector and pre-miR-27a. Furthermore, the presence of exogenous miR-27a-3p significantly decreased Wnt3a protein expression rather than mRNA and reduced β-catenin mRNA levels in melanocytes. The over-expression of miR-27a-3p significantly increased the melanin content of melanocytes. However, miR-27a-3p inhibitor performs an opposite effect on melanogenesis. Wnt3a is one target of miR-27a-3p. MiR-27a-3p could inhibit Wnt3a protein amount by post-transcriptional regulation and melanogenesis in mouse melanocytes. Previous studies reported that Wnt3a promoted melanogenensis in mouse melanocytes. Thus, miR-27-3p inhibits melanogenesis by repressing Wnt3a protein expression. 4. Searches for Exotic Decays of the Upsilon(3S) at BaBar Energy Technology Data Exchange (ETDEWEB) Hooberman, Benjamin; /LBL, Berkeley /Heidelberg U. 2011-12-01 In this paper we present two searches for new physics in {Upsilon}(3S) decays collected by the BABAR detector. We search for charged lepton-flavour violating decays of the {Upsilon}(3S), which are unobservable in the Standard Model but are predicted to occur in several beyond-the-Standard Model scenarios. We also search for production of a light Higgs or Higgs-like state produced in radiative decays of the {Upsilon}(3S) and decaying to muon pairs. 5. Mechanisms and Kinetics of Radical Reaction of O(1D,3P) + HCN System Institute of Scientific and Technical Information of China (English) HUANG Yu-Cheng; DU Jin-Yan; JU Xue-Hai; YE Shi-Yong; ZHOU Tao 2008-01-01 The reaction of HCN with O(1D, 3P) radical has been investigated by density functional theory (DFT) and ab initio methods. The stationary points on the reaction paths(reactants, intermediates and products) were optimized at the (U)B3LYP/aug-cc-pVTZ level.Single-point calculations were performed at the (U)QCISD(T)/aug-cc-pVTZ level for the optimized structures and all the total energies were corrected by zero-point energy. It is shown that there exist three competing mechanisms of oxygen attacking nitrogen O→N, oxygen attacking carbon O→C and oxygen attacking hydrogen O→H. The rate constants were obtained via Eyring transition-state theory in the temperature range of 600～2000 K. The linear relationship between lnk and 1/T was presented. The results show that path 1 is the main reaction channel and the product of NCO + H is predominant. 6. Narrow-linewidth cooling of ^{6}Li atoms using the 2S-3P transition CERN Document Server Chen, Hao-Ze; Wu, Yu-Ping; Liu, Xiang-Pei; Wang, Xiao-Qiong; Chen, Yu-Ao; Pan, Jian-Wei 2016-01-01 We report on a narrow-linewidth cooling of ^{6}Li atoms using the 2S_{1/2}\\to 3P_{3/2} transition in the ultraviolet (UV) wavelength regime. By combining the traditional red magneto-optical trap (MOT) at 671 nm and the UV MOT at 323 nm, we obtain a cold sample of 1.3\\times10^9 atoms with a temperature of 58 \\muK. Furthermore, we demonstrate a high efficiency magnetic transport for ^{6}Li atoms with the help of the UV MOT. Finally, we obtain 8.1\\times10^8 atoms with a temperature of 296 \\muK at a magnetic gradient of 198 G/cm in the science chamber with a good vacuum environment and large optical access. 7. C-H activation of ethers by pyridine tethered PCsp3P-type iridium complexes. Science.gov (United States) Cui, Peng; Babbini, Dominic C; Iluc, Vlad M 2016-06-14 Iridium PCsp3P complexes featuring a novel bis(2-diphenylphosphinophenyl)-2-pyridylmethane ligand (PC(Py)HP) are reported. C-H activation reactions between the dihydride complex [(PC(Py)P)Ir(H)2] and tetrahydrofuran or methyl tert-butyl ether in the presence of a hydrogen acceptor, norbornene (NBE), at ambient temperature led exclusively to the hydrido oxyalkyl complexes, [(PC(Py)P)IrH(C4H7O)] and [(PC(Py)P)IrH(CH2O(t)Bu)], respectively. The internal pyridine donor is important and stabilizes these species by coordination to the iridium center. The coordination of pyridine to the iridium center is labile, however, and its dissociation occurs in the presence of a suitable substrate, as demonstrated by the intramolecular nucleophilic attack of pyridine on a vinylidene intermediate generated from PhC[triple bond, length as m-dash]CH. 8. Product lambda-doublet ratios for the O(3P) + D2 reaction: A mechanistic imprint CERN Document Server Jambrina, P G; Aldegunde, J; Brouard, M; Aoiz, F J 2016-01-01 In the last decade, the development of theoretical methods have allowed chemists to reproduce and explain almost all of the experimental data associated with elementary atom plus diatom collisions. However, there are still a few examples where theory cannot account yet for experimental results. This is the case for the preferential population of one of the \\Lambda-doublet states produced by chemical reactions. In particular, recent measurements of the OD(^2\\Pi) product of the O(^3P) + D_2 reaction have shown a clear preference for the \\Pi(A') \\Lambda-doublet states, in apparent contradiction with {\\em ab initio} calculations, which predict a larger reactivity on the A" potential energy surface. Here we present a method to calculate the \\Lambda-doublet ratio when concurrent potential energy surfaces participate in the reaction. It accounts for the experimental \\Lambda-doublet populations via explicit consideration of the stereodynamics of the process. Furthermore, our results demonstrate that... 9. First-principles investigation of Cu-doped ZnS with enhanced photocatalytic hydrogen production activity Science.gov (United States) Dong, Ming; Zhou, Peng; Jiang, Chuanjia; Cheng, Bei; Yu, Jiaguo 2017-01-01 The band structure and electronic properties of Cu-doped wurtzite ZnS were investigated by density functional theory calculations. According to the formation energies, the substitutional Cu and S vacancy defects are stable among the examined doping species. Particularly, the hybridization of substitutional Cu 3d and S 3p orbitals narrows the band gap of substitutional Cu-doped ZnS (CuZn-ZnS), while the high effective mass ratio of photogenerated holes and electrons (mh∗/me∗) in the CuZn-ZnS is beneficial for the separation and migration of the photogenerated charge carriers. Lab-synthesized CuZn-ZnS sample exhibited enhanced visible-light absorption and photocatalytic hydrogen production activity compared to pure ZnS. 10. Morphology controlled synthesis of 2-D Ni-Ni3S2 and Ni3S2 nanostructures on Ni foam towards oxygen evolution reaction Science.gov (United States) Chaudhari, Nitin Kaduba; Oh, Aram; Sa, Young Jin; Jin, Haneul; Baik, Hionsuck; Kim, Sang Gu; Lee, Suk Joong; Joo, Sang Hoon; Lee, Kwangyeol 2017-03-01 Catalysts for oxygen evolution reactions (OER) are at the heart of key renewable energy technologies, and development of non-precious metal catalysts with high activity and stability remain a great challenge in this field. Among various material candidates, metal sulfides are receiving increasing attention. While morphology-dependent catalytic performances are well established in noble metal-based catalysts, relatively little is known for the morphology‒catalytic performance relationship in metal sulfide catalysts. In this study, uniform spider web-like Ni nanosheets-Ni3S2 and honeycomb-like Ni3S2 structures are deposited on nickel foam (Ni3S2/NF) by a facile one-step hydrothermal synthetic route. When used as an oxygen evolution electrode, the spider web-like Ni-Ni3S2/NF with the large exposed surface area shown excellent catalytic activity and stability with an overpotential of 310 mV to achieve at 10 mA/cm2 and a Tafel slope of 63 mV/dec in alkaline media, which is superior to the honeycomb-like structure without Ni nanosheet. The low Tafel slope of the spider web-like Ni-Ni3S2/NF represents one of the best OER kinetics among nickel sulfide-based OER catalysts. The results point to the fact that performance of the metal sulfide electrocatalysts might be fine-tuned and optimized with morphological controls. 11. CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES NARCIS (Netherlands) MICHAELIS, SC; BARDENHEUER, W; LUX, A; SCHRAMM, A; GOCKEL, A; SIEBERT, R; WILLERS, C; SCHMIDTKE, K; TODT, B; VANDERHOUT, AH; BUYS, CHCM; HEPPELLPARTON, AC; RABBITTS, PH; UNGAR, S; SMITH, D; LEPASLIER, D; COHEN, D; OPALKA, B; SCHUTTE, J 1995-01-01 Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, and other neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a 12. DEPENDENCE OF ELECTRON-CAPTURE FROM NA-ASTERISK(3P) ON ORBITAL ALIGNMENT - A KINEMATIC EFFECT NARCIS (Netherlands) SCHLATMANN, AR; MORGENSTERN, R 1993-01-01 Recent experimental data of electron capture into He+(4l) states from SIGMA and PI aligned Na*(3p) orbitals are interpreted. Calculations of the overlap integral of the initial Na*(3p) and the final He+(4l) wavefunctions in momentum space reproduce the velocity dependence of the measured cross secti 13. STRONG VELOCITY DEPENDENCE OF ELECTRON-CAPTURE IN COLLISIONS BETWEEN ALIGNED NA-ASTERISK(3P) AND HE-2+ NARCIS (Netherlands) SCHLATMANN, AR; HOEKSTRA, R; MORGENSTERN, R; OLSON, RE; PASCALE, J 1993-01-01 By analyzing spectra of emitted photons, we have studied state-selective electron capture in collisions of He2+ on aligned Na*(3p) atoms that span the ''velocity-matching'' energy between projectile and target electron. We find a strong dependence of the capture cross sections on the Na*(3p) orbital 14. Amino acid sequence alignment of vertebrate CAPN3/calpain-3/p94 Directory of Open Access Journals (Sweden) Yasuko Ono 2015-12-01 Full Text Available CAPN3 is a calpain superfamily member that is predominantly expressed in skeletal muscle. So far, clear CAPN3 orthologs were found only in vertebrates. CAPN3 is a unique protease in that it undergoes extremely rapid and exhaustive autolysis and that autolyzed fragments spontaneously associate each other to reconstitute the proteolytic activity. These unique properties of CAPN3 are dependent on IS1 and IS2, two CAPN3-characterizing sequences that do not exist in other calpains or any other proteases. To understand how IS1 and IS2 are conserved among vertebrates, this data article provides amino acid sequence alignment of representative vertebrate CAPN3s. For further analysis and discussion, see Ono et al. [1 15. First-principles study of electronic structure of CuSbS{sub 2} and CuSbSe{sub 2} photovoltaic semiconductors Energy Technology Data Exchange (ETDEWEB) Maeda, T., E-mail: tmaeda@ad.ryukoku.ac.jp; Wada, T. 2015-05-01 We studied the features of CuSbS{sub 2} (CAS) and CuSbSe{sub 2} (CASe), two proposed photovoltaic compounds, and clarified their electronic structures by first-principles calculations and compared them to the chalcopyrite-type CuInSe{sub 2} results. For both CAS and CASe, the calculated enthalpies of formation of the chalcostibite phases were considerably lower than those of the chalcopyrite phases. Therefore, we considered that the chalcostibite phase is more stable for CAS and CASe. In their band structure calculated with the HSE06 hybrid functional, the valence band maxima of CAS and CASe were located at the Γ-point, and the conduction band minima were located at the R-point. Their second lowest conduction band was located at the Γ-point, whose energy level nearly equaled the R-point. For CAS (CASe), the partial density of the states shows the character of the Cu 3d and S 3p (Se 4p) orbitals at the top of the valence bands and the Sb 5p and S 3p (Se 4p) orbitals at the bottom of the conduction bands. The conduction bands of CAS and CASe have a p-orbital character (Sb 5p) that differs from the s-orbital character (In 5s) of CuInSe{sub 2}. It is for the reason that CAS and CASe do not have a chalcopyrite structure but a chalcostibite-type structure. The calculated absorption coefficient of CuSbS{sub 2} (10{sup 4}-10{sup 5} cm{sup −1}) is comparable to that of CuInSe{sub 2}. - Highlights: • We studied the features of CuSbS{sub 2} and CuSbSe{sub 2}, newly proposed photovoltaic compounds. • Chalcostibite phase is more stable in CuSbS{sub 2} and CuSbSe{sub 2}. • Band structures of CuSbS{sub 2} and CuSbS{sub 2} were calculated with HSE06 hybrid functional. • Absorption coefficient of chalcostibite-type CuSbS{sub 2} is comparable to that of CuInSe{sub 2}. 16. MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1 Energy Technology Data Exchange (ETDEWEB) Gu, Haihua; Yang, Tao; Fu, Shaozi; Chen, Xiaofan; Guo, Lei; Ni, Yiming, E-mail: ni_yiming@hotmail.com 2014-01-31 Highlights: • We examined the level of miR-490-3p in A549 lung cancer cells compared with normal bronchial epithelial cell line. • We are the first to show the function of miR-490-3p in A549 lung cancer cells. • We demonstrate CCND1 may be one of the targets of miR-490-3p. - Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate the translation of messenger RNAs by binding their 3′-untranslated region (3′UTR). In this study, we found that miR-490-3p is significantly down-regulated in A549 lung cancer cells compared with the normal bronchial epithelial cell line. To better characterize the role of miR-490-3p in A549 cells, we performed a gain-of-function analysis by transfecting the A549 cells with chemically synthesized miR-490-3P mimics. Overexpression of miR-490-3P evidently inhibits cell proliferation via G1-phase arrest. We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1, which plays a key role in G1/S phase transition. In addition, the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3′UTR. These findings indicated miR-490-3P could be a potential suppressor of cellular proliferation. 17. H2S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression Science.gov (United States) Li, Xing-Hui; Xue, Wen-Long; Wang, Ming-Jie; Zhou, Yu; Zhang, Cai-Cai; Sun, Chen; Zhu, Lei; Liang, Kun; Chen, Ying; Tao, Bei-Bei; Tan, Bo; Yu, Bo; Zhu, Yi-Chun 2017-01-01 The aims of the present study are to determine whether hydrogen sulfide (H2S) is involved in the expression of endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) production, and to identify the role of microRNA-455-3p (miR-455-3p) during those processes. In cultured human umbilical vein endothelial cells (HUVECs), the expression of miR-455-3p, eNOS protein and the NO production was detected after administration with 50 μM NaHS. The results indicated that H2S could augment the expression of miR-455-3p and eNOS protein, leading to the increase of NO level. We also found that overexpression of miR-455-3p in HUVECs increased the protein levels of eNOS whereas inhibition of miR-455-3p decreased it. Moreover, H2S and miR-455-3p could no longer increase the protein level of eNOS in the presence of proteasome inhibitor, MG-132. In vivo, miR-455-3p and eNOS expression were considerably increased in C57BL/6 mouse aorta, muscle and heart after administration with 50 μmol/kg/day NaHS for 7 days. We also identified that H2S levels and miR-455-3p expression increased in human atherosclerosis plaque while H2S levels decreased in plasma of atherosclerosis patients. Our data suggest that the stability of eNOS protein and the NO production could be regulated by H2S through miR-455-3p. PMID:28322298 18. Delafossite-CuAlO{sub 2} films prepared by annealing of amorphous Cu-Al-O films at high temperature under controlled atmosphere Energy Technology Data Exchange (ETDEWEB) Chen, Hong-Ying, E-mail: hychen@cc.kuas.edu.tw; Tsai, Ming-Wei 2011-07-01 In this study, amorphous Cu-Al-O films were deposited onto a (100) p-type silicon substrate by a magnetron sputtering system. The films were then annealed at 700 deg. C and 800 deg. C for 2 h in N{sub 2}, air and O{sub 2}. X-ray diffraction patterns showed that the as-deposited films were amorphous. When the films were annealed at 700 deg. C, the monoclinic-CuO and spinel-CuAl{sub 2}O{sub 4} phases were detected in all atmospheres. As the annealing temperature increased to 800 deg. C, delafossite-CuAlO{sub 2} (R3-bar m and P6{sub 3}/mmc phases) appeared in N{sub 2} whereas monoclinic-CuO and spinel-CuAl{sub 2}O{sub 4} phases were detected in air and O{sub 2}. Thermodynamic calculations can explain the formation of delafossite-CuAlO{sub 2} films. The optical bandgap and conductivity of delafossite-CuAlO{sub 2} films were 3.30 eV and 6.8 x 10{sup -3} S/cm, respectively, which are compatible with other data in the literature. The p-type characteristic in delafossite-CuAlO{sub 2} films was verified by a hot-probe method. 19. Multifunctional g3p-peptide tag for current phage display systems. Science.gov (United States) Beckmann, C; Haase, B; Timmis, K N; Tesar, M 1998-03-15 We have previously described a monoclonal antibody (mAb), 10C3, directed against the gene-3 protein (g3p) of filamentous phage M13, which was produced to study g3p fusion protein expression in Escherichia coli and its incorporation in the phage capsid [Tesar, M., Beckmann, C., Röttgen, P., Haase, B., Faude, U., Timmis, K., 1995. Monoclonal antibody against pIII of filamentous phage: an immunological tool to study pIII fusion protein expression in phage display systems. Immunology 1, 53-54]. In this study we report mapping of the antigenic epitope of the mAb 10C3, by means of short overlapping peptide-sequences [Frank, R., Overwin, H., 1996. Spot synthesis. In: Morris, G.E. (Ed.), Methods in Molecular Biology, Vol. 66: Epitope Mapping Protocols. Humana Press, Totowa, NJ, pp. 149-169.] comprising the C-terminal half of the g3-protein. A minimal recognizable peptide was found which is represented in the 11 amino acid sequence from positions 292 to 302 of g3p [Wezenbeek van, P.M.G.P., Hulsebos, T.J.M., Schoenmakers, J.G.G., 1980. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene 11, 129-148]. In order to use the antibody also for detection and purification of recombinant proteins, such as single chain antibodies, the epitope was introduced as a tag sequence into the phagemid pHEN1 [Hoogenboom, H.R., Griffith, A.D., Johnson, K., Chiswell, D.J., Hudson, P., Winter, G., 1991. Multi-subunit proteins on the surface of the filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acid Res. 19, 4133-4137; Nissim, A., Hoogenboom, H.R., Tomlinson, I.M., Flynn, G., Midgley, C., Lane, D., Winter, G., 1994. Antibody fragments from a single pot phage display library as immunochemical reagents. EMBO J. 13 (3) 692-698]. Purified single chain antibodies containing this tag were detectable down to a concentration of 2 ng ml(-1) under non-denaturing conditions (ELISA) or 4 ng per lane on immunoblots 20. miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy. Science.gov (United States) Tan, Shifan; Shi, Huijuan; Ba, Mingchen; Lin, Shengqv; Tang, Hongsheng; Zeng, Xiaoqi; Zhang, Xiangliang 2016-04-01 The chemoresistance of colon cancer cells limits the efficacy of chemotherapy. miR-409-3p has been shown to be downregulated in various types of cancer. In the present study, we examined the role of miR-409-3p in colon cancer as well as the effects of miR‑409-3p on the sensitivity of colon cancer cells to oxaliplatin. The expression of miR-409 was significantly downregulated in the human colon cancer cell lines compared with the normal colon epithelial cells. Importantly, the miR-409-3p expression levels were lower in human colon cancer patient samples than in normal colon tissues. Moreover, we observed a negative correlation between the miR‑409-3p levels and resistance to oxaliplatin: the oxaliplatin-resistant colon cancer cells exhibited significantly downregulated miR‑409-3p levels, but higher autophagic activity than the oxaliplatin-sensitive cells. Using bioinformatics analysis, we predicted that miR‑409-3p miRNA binds to the key autophagy gene encoding Beclin-1. Our findings indicated that the overexpression of miR‑409-3p inhibited Beclin-1 expression and autophagic activity by binding to the 3'-untranslated region of Beclin-1 mRNA. In addition, the overexpression of miR‑409-3p enhanced the chemosensitivity of the oxaliplatin-sensitive and oxaliplatin-resistant colon cancer cells. The restoration of Beclin-1 abrogated these effects of miR‑409-3p. In a xenograft model using nude mice, we examined the effects of miR‑409-3p on tumor growth during chemotherapy. miR‑409-3p overexpression sensitized the tumor to chemotherapy, while inhibiting chemotherapy-induced autophagy in a manner dependent on Beclin-1. The findings of our study suggest that miR-409-3p is capable of enhancing the chemosensitivity of colon cancer cells by inhibiting Beclin-1-mediated autophagy. 1. Crystal structure of methyl (2R,3S-3-[(tert-butylsulfinylamino]-2-fluoro-3-phenylpropanoate Directory of Open Access Journals (Sweden) Zhiwei Zhao 2015-12-01 Full Text Available The title compound, C14H20FNO3S, contains two chiral carbon centres and the absolute configuration has been confirmed as (2R,3S. In the crystal, adjacent molecules are linked by weak C—H...O hydrogen bonds, generating zigzag chains along the a-axis direction. 2. /Cu-Al System Science.gov (United States) Kish, Orel; Froumin, Natalya; Aizenshtein, Michael; Frage, Nachum 2014-05-01 Wettability and interfacial interaction of the Ta2O5/Cu-Al system were studied. Pure Cu does not wet the Ta2O5 substrate, and improved spreading is achieved when relatively a high fraction of the active element (~40 at.% Al) was added. The Al2O3 and AlTaO4 phases were observed at the Ta2O5/Cu-Al interface. A thermodynamic evaluation allowed us to suggest that the lack of wetting bellow 40 at.% Al is due to the presence of a native oxide, which covers the drop. The conditions of the native oxide decomposition and the formation of the volatile Al2O suboxide strongly depend on the vacuum level during sessile drop experiments and the composition of the Cu-Al alloy. In our case, Al contents greater than 40% provides thermodynamic conditions for the formation of Al2O (as a result of Al reaction with Al2O3) and the drop spreading. It was suggested that the final contact angle in the Ta2O5/Cu-Al system (50°) is determined by Ta adsorption on the newly formed alumina interlayer. 3. miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200 Science.gov (United States) Fang, Wang; Shu, Shan; Yongmei, Li; Endong, Zhu; Lirong, Yin; Bei, Sun 2016-01-01 Cervical cancer (CC) is a malignant solid tumor, which is one of the main causes of morbidity and mortality in women. Persistent High-risk human papillomavirus (hrHPV) infection is closely related to cervical cancer and autophagy has been suggested to inhibit viral infections. miRNAs have been reported to regulate autophagy in many solid tumors with many studies implicating miR-224-3p in the regulation of autophagy. In this study, we performed a miRNA microarray analysis on CC tissues and found that a large number of miRNAs with differential expressions in hrHPV-infected tissues. We identified miR-224-3p as a candidate miRNA selectively up regulated in HPV-infected tissues and cell lines. Further analysis revealed that miR-224-3p regulates autophagy in cervical cancer tissues and cell lines. While the overexpression of miR-224-3p inhibits autophagy in HPV-infected cells, knocking down endogenous miR-224-3p increases autophagy activity in the same cells. In addition, we found that miR-224-3p directly inhibits the expression of autophagy related gene, FAK family-interacting protein of 200 kDa (FIP200). In summary, we found that miR-224-3p regulates autophagy in hrHPV-induced cervical cancer cells through targeting FIP200 expression. PMID:27615604 4. miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells Directory of Open Access Journals (Sweden) Zhong-Yan Chen 2017-01-01 Full Text Available MicroRNAs (miRNAs play important roles in cell fate decisions. However, the miRNAs and their targets involved in the regulation of cardiac lineage specification are largely unexplored. Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mESCs. With a miRNA array screen, we identified a number of miRNAs significantly changed during mESC differentiation into the mesodermal and cardiac progenitor cells, and miR-142-3p was one among the markedly downregulated miRNAs. Ectopic expression and inhibition of miR-142-3p did not alter the characteristics of undifferentiated ESCs, whereas ectopic expression of miR-142-3p impaired cardiomyocyte formation. In addition, ectopic expression of miR-142-3p inhibited the expression of a cardiac mesodermal marker gene Mesp1 and downstream cardiac transcription factors Nkx2.5, Tbx5, and Mef2c but not the expression of three germ layer-specific genes. We further demonstrated that miR-142-3p targeted the 3′-untranslated region of Mef2c. These results reveal miR-142-3p as an important regulator of early cardiomyocyte differentiation. Our findings provide new knowledge for further understanding of roles and mechanisms of miRNAs as critical regulators of cardiomyocyte differentiation. 5. MicroRNA-338-3p suppresses tumor growth of esophageal squamous cell carcinoma in vitro and in vivo. Science.gov (United States) Li, Xinyu; Li, Zhihong; Yang, Guiyun; Pan, Zhenxiang 2015-09-01 Accumulating evidence has shown that microRNAs (miRNAs) are aberrantly expressed in human esophageal squamous cell carcinoma (ESCC) and are crucial in tumorigenesis, among which miR‑338‑3p has been examined to be downregulated in patients with ESCC. However, the role of miR‑338‑3p in ESCC remains to be elucidated. In the present study, the role of miR‑338‑3p on the growth and survival of an ESCC cell line was determined with several in vitro approaches and in nude mouse models. It was determined that miR‑338‑3p expression was frequently downregulated in ESCC tissue compared with corresponding adjacent non‑tumor tissue, and that its expression was significantly correlated with tumor stage and metastasis. Overexpression of miR‑338‑3p in ESCC cells suppressed cell proliferation, colony formation, migration and invasion, and induced cell arrest at the G0/G1 stage and cell apoptosis in vitro. In addition, it was demonstrated that overexpression of miR‑338‑3p significantly suppresses tumor growth of xenograft tumors in mice (PESCC, and its dysregulation may be involved in the initiation and development of human ESCC. In addition, it was suggested that miR‑338‑3p may be a potential therapeutic agent for treatment of ESCC. 6. Functional expression of pig renal organic anion transporter 3 (pOAT3). Science.gov (United States) Hagos, Yohannes; Braun, Isabella M; Krick, Wolfgang; Burckhardt, Gerhard; Bahn, Andrew 2005-05-01 With the cloning of pig renal organic anion transporter 1 (pOAT1) (Biochimie 84 (2002) 1219) we set up a model system for comparative studies of cloned and natively isolated membrane located transport proteins. Meanwhile, another transport protein involved in p-aminohippurate (PAH) uptake on the basolateral side of the proximal tubule cells was identified, designated organic anion transporter 3 (OAT3). To explore the contribution of pOAT1 to the PAH clearance in comparison to OAT3, it was the aim of this study to extend our model by cloning of the pig ortholog of OAT3. Sequence comparisons of human organic anion transporter 3 (hOAT3) with the expressed sequence tag (EST) database revealed a clone and partial sequence of the pig renal organic anion transporter 3 (pOAT3) ortholog. Sequencing of the entire open reading frame resulted in a protein of 543 amino acid residues encoded by 1632 base pairs (EMBL Acc. No. AJ587003). It showed high homologies of 81%, 80%, 76%, and 77% to the human, rabbit, rat, and mouse OAT3, respectively. A functional characterization of pOAT3 in Xenopus laevis oocytes yielded an apparent Km (Kt) for [3H]estrone sulfate of 7.8 +/- 1.3 microM. Moreover, pOAT3 mediated [3H]estrone sulfate uptake was almost abolished by 0.5 mM of glutarate, dehydroepiandosterone sulfate, or probenecid consistent with the hallmarks of OAT3 function. 7. Excitons into one-axis crystals of zinc phosphide (Zn3P2 Directory of Open Access Journals (Sweden) D.M. Stepanchikov 2009-01-01 Full Text Available Theoretical study of excitons spectra is offered in this report as for Zn3P2 crystals. Spectra are got in the zero approach of the theory of perturbations with consideration of both the anisotropy of the dispersion law and the selection rules. The existence of two exciton series was found, which corresponds to two valence bands (hh, lh and the conductivity band (c. It is noteworthy that anisotropy of the dispersion law plus the existence of crystalline packets (layers normal to the main optical axis, both will permit the consideration of two-dimensional excitons too. The high temperature displaying of these 2D-exciton effects is not eliminated even into bulk crystals. The calculated values of the binding energies as well as the oscillator's strength for the optical transitions are given for a volume (3D and for two-dimensional (2D excitons. The model of energy exciton transitions and four-level scheme of stimulated exciton radiation for receiving laser effect are offered. 8. Dynamics of the gas-liquid interfacial reaction of O(3P) atoms with hydrocarbons Science.gov (United States) Kelso, Hailey; Köhler, Sven P. K.; Henderson, David A.; McKendrick, Kenneth G. 2003-11-01 We describe an experimental approach to the determination of the nascent internal state distribution of gas-phase products of a gas-liquid interfacial reaction. The system chosen for study is O(3P) atoms with the surface of liquid deuterated squalane, a partially branched long-chain saturated hydrocarbon, C30D62. The nascent OD products are detected by laser-induced fluorescence. Both OD (v'=0) and (v'=1) were observed in significant yield. The rotational distributions in both vibrational levels are essentially the same, and are characteristic of a Boltzmann distribution at a temperature close to that of the liquid surface. This contrasts with the distributions in the corresponding homogeneous gas-phase reactions. We propose a preliminary interpretation in terms of a dominant trapping-desorption mechanism, in which the OD molecules are retained at the surface sufficiently long to cause rotational equilibration but not complete vibrational relaxation. The significant yield of vibrationally excited OD also suggests that the surface is not composed entirely of -CD3 endgroups, but that secondary and/or tertiary units along the backbone are exposed. 9. Theoretical investigation of hyperthermal reactions at the gas-liquid interface: O (3P) and squalane. Science.gov (United States) Kim, Dongwook; Schatz, George C 2007-06-14 Hyperthermal collisions (5 eV) of ground-state atomic oxygen [O ((3)P)] with a liquid-saturated hydrocarbon, squalane (C(30)H(62)), have been studied using QM/MM hybrid "on-the-fly" direct dynamics. The surface structure of the liquid squalane is obtained from a classical molecular dynamics simulation using the OPLS-AA force field. The MSINDO semiempirical Hamiltonian is combined with OPLS-AA for the QM/MM calculations. In order to achieve a more consistent and efficient simulation of the collisions, we implemented a dynamic partitioning of the QM and MM atoms in which atoms are assigned to QM or MM regions based on their proximity to "seed" (open-shell) atoms that determine where bond making/breaking can occur. In addition, the number of seed atoms is allowed to increase or decrease as time evolves so that multiple reactive events can be described. The results show that H abstraction is the most important process for all incident angles, with H elimination, double H abstraction, and C-C bond cleavage also being important. A number of properties of these reactive channels, as well as inelastic nonreactive scattering, are investigated, including angular and translational energy distributions, the effect of incident collision angle, variation with depth of the reactive event within the liquid, with the reaction site on the hydrocarbon, and the effect of dynamics before and after reaction (direct reaction versus trapping reaction-desorption). 10. Dynamics of interfacial reactions between O(3 P) atoms and long-chain liquid hydrocarbons Science.gov (United States) Allan, Mhairi; Bagot, Paul A. J.; Köhler, Sven P. K.; Reed, Stewart K.; Westacott, Robin E.; Costen, Matthew L.; McKendrick, Kenneth G. 2007-09-01 Recent progress that has been made towards understanding the dynamics of collisions at the gas-liquid interface is summarized briefly. We describe in this context a promising new approach to the experimental study of gas-liquid interfacial reactions that we have introduced. This is based on laser-photolytic production of reactive gas-phase atoms above the liquid surface and laser-spectroscopic probing of the resulting nascent products. This technique is illustrated for reaction of O(3P) atoms at the surface of the long-chain liquid hydrocarbon squalane (2,6,10,15,19,23-hexamethyltetracosane). Laser-induced fluorescence detection of the nascent OH has revealed mechanistically diagnostic correlations between its internal and translational energy distributions. Vibrationally excited OH molecules are able to escape the surface. At least two contributions to the product rotational distributions are identified, confirming and extending previous hypotheses of the participation of both direct and trapping-desorption mechanisms. We speculate briefly on future experimental and theoretical developments that might be necessary to address the many currently unanswered mechanistic questions for this, and other, classes of gas-liquid interfacial reaction. 11. Join the CERN ISEF special award winners | 16 June - 3 p.m. CERN Multimedia 2016-01-01 Come and join the CERN ISEF special award winners at their lightning talks session on 16 June at 3.00 p.m. in the main auditorium. The 2016 Intel ISEF CERN special award winners on stage with the selection committee on 17 May 2016 in Phoenix, Arizona, USA. (Picture: Society for Science and the Public) Between 11 and 17 June 2016, the ten finalists of the Intel International Science and Engineering Fair (ISEF) who won the CERN Special Award, will visit CERN to partake in various educational lectures. ISEF is the world's largest international pre-college science competition, with approximately 1,700 high school students from more than 75 countries taking part. They will present their projects in short 5 minutes lightning talks' sessions at the main auditorium on Thursday 16 June at 3 p.m. The award winners would be also very happy to have a chance to interact and discuss with you af... 12. Utilising the `3P-model' to Characterise the Discipline of Didactics of Science Science.gov (United States) Adúriz-Bravo, Agustín; Izquierdo-Aymerich, Mercè In our research within didactics of science, we have been exploring contributions of the so called cognitive models from contemporary philosophy of science. We have used these philosophical frameworks on different levels. As an outcome, we have formulated a model of didactics of science according to which this discipline adapts and transforms theoretical contributions from different scholarly fields. In this paper, we concentrate on this description of didactics of science, which we have called the 3P-model (i.e., philosophy + psychology + pedagogy). This model of the internal functioning of the discipline may be useful to make innovations in science curriculum design and re-conceptualise the role of science teachers as professionals. We see didactics of science as a set of interrelated activities, performed by different individuals, and ranging from theoretical production to practice of science education at school. We find the concept of technoscience suitable to account for this diversity of goals. According to this concept, scientific disciplines are identified both with generation of knowledge and with active intervention on the world. Within current didactics of science, we recognise several kinds of research, having goals more or less directed to practical intervention in science education. 13. Precision frequency measurement of 1S0-3P1 intercombination lines of Sr isotopes Science.gov (United States) Liu, Hui; Gao, Feng; Ye-Bing, Wang; Xiao, Tian; Jie, Ren; Ben-Quan, Lu; Qin-Fang, Xu; Yu-Lin, Xie; Hong, Chang 2015-01-01 We report on frequency measurement of the intercombination (5s2)1S0-(5s5p)3P1 transition of the four natural isotopes of strontium, including 88Sr (82.58%), 87Sr (7.0%), 86Sr (9.86%), and 84Sr (0.56%). A narrow-linewidth laser that is locked to an ultra-low expansion (ULE) optical cavity with a finesse of 12000 is evaluated at a linewidth of 200 Hz with a fractional frequency drift of 2.8×10-13 at an integration time of 1 s. The fluorescence collector and detector are specially designed, based on a thermal atomic beam. Using a double-pass acousto-optic modulator (AOM) combined with a fiber and laser power stabilization configuration to detune the laser frequency enables high signal-to-noise ratios and precision saturated spectra to be obtained for the six transition lines, which allows us to determine the transition frequency precisely. The optical frequency is measured using an optical frequency synthesizer referenced to an H maser. Both the statistical values and the final values, including the corrections and uncertainties, are derived for a comparison with the values given in other works. Project supported by the National Natural Science Foundation of China (Grant No. 61127901) and the Key Project of the Chinese Academy of Sciences (Grant No. KJZD-EW-W02). 14. miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression Energy Technology Data Exchange (ETDEWEB) Yu, Peng; Wu, Dingguo; You, Yu; Sun, Jing; Lu, Lele; Tan, Jiaxing; Bie, Ping, E-mail: bieping2010@163.com 2015-08-15 MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment. - Highlights: • miR-208-3p was highly expressed and directly repressed the expression of ARID2 in HCC. • miR-208-3p contributed to HCC cell progression both in vitro and in vivo. • Over-expression of ARID2 inhibited the HCC cell proliferation and invasion. • Restoration of ARID2 partly reversed the the effect of miR-208-3p down-regulation on HCC cells. • Newly regulatory pathway: miR-208-3p mediated the repression of ARID2 by TGFβ1 in HCC cells. 15. Syntheses of heterometallic V/Cu/S clusters and structural characterization of（Et4N）2[V2Cu2S4(C5H10dtc)2 Institute of Scientific and Technical Information of China (English) 刘秋田; 杨瑜; 黄梁仁; 吴达旭; 康北笙; 陈昌能; 邓玉恒; 卢嘉锡 1995-01-01 Three kinds of complexes,V2Cu2S4 cubane-like,VS4Cu4 planar and V3S7 triangular dusters areobtained from a reaction system composed of（NH4）3VS4,CuCl,R2dtcNa and PhSNa.A mechanism of theformation of the products is suggested.The title compound is structurally characterized,showing the metaloxidation states of V（IV）+Cu（I）.Also discussed are IR,UV-Vis and NMR spectra,and cyclic voltagrammetryfor the V2Cu2S4 clusters. 16. Dealloying of Cu{sub x}Au studied by hard X-ray photoelectron spectroscopy Energy Technology Data Exchange (ETDEWEB) Rajput, Parasmani, E-mail: parasmani.rajput@northwestern.edu [European Synchrotron Radiation Facility, 6 rue Jules Horowitz, F-38043 Grenoble (France); Gupta, Ajay [UGC-DAE Consortium for Scientific Research, University Campus, Khandwa Road, Indore 452 017 (India); Detlefs, Blanka [European Synchrotron Radiation Facility, 6 rue Jules Horowitz, F-38043 Grenoble (France); Kolb, Dieter M. [Institute for Electrochemistry, University of Ulm, D-89069 Ulm (Germany); Potdar, Satish [UGC-DAE Consortium for Scientific Research, University Campus, Khandwa Road, Indore 452 017 (India); Zegenhagen, Jörg [European Synchrotron Radiation Facility, 6 rue Jules Horowitz, F-38043 Grenoble (France) 2013-10-15 Highlights: ► The shift in binding energy of Cu and Au lines in CuAu alloys is opposite to expected from the nobility of the elements. ► The magnitude of the chemical shifts of the metal lines in CuAu alloys is strongly influenced by finite size effects and disorder. ► Cu 3s and/or Au 4f cross-sections are not well described by theory (Scofield). The Cu 3s photoabsorption cross-section seems to be strongly overestimated. ► We find/confirm that (CuAu) dealloying proceeds into depth like a spinodal decomposition. -- Abstract: We studied pristine and leached ultra-thin Cu{sub x}Au (x ≈ 4) films by hard X-ray photoelectron spectroscopy. The Au 4f and Cu 3s core levels show a shift in binding energy which is opposite to expected from the nobility of the elements, which is explained by charge transfer involving differently screening s and d valence levels of the elements [W. Eberhardt, S.C. Wu, R. Garrett, D. Sondericker, F. Jona, Phys. Rev. B 31 (1985) 8285]. The magnitude of the chemical shifts of the metal lines is strongly influenced by the finite size and disorder of the films. Angular dependent photoelectron emission allowed to assess the alloy composition as a function of depth larger than 5 nm. The potential controlled dealloying proceeds into depth like a spinodal decomposition with Cu going into solution and the remaining Au accumulating in the surface region. The compositional gradient did not lead to a significant broadening of the metal photoelectron lines suggesting a non-local screening mechanism. 17. 共沉默miR-221-3p/222-3p表达抑制骨髓间充质干细胞增殖及促成软骨分化%Down-regulation of miR-221-3p/222-3p inhibits cell proliferation and promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells Institute of Scientific and Technical Information of China (English) 闫继红; 周德山; 杨姝; 孙海梅; 曹丹丹; 张秀英; 季凤清; 郭多; 吴波; 孙婷怡 2015-01-01 BACKGROUND:The use of mesenchymal stem cels in the field of tissue engineering for osteoarticular injury repair is a very promising tool since these cels are readily expandable and able to differentiate into chondrocytes. Abundant evidence suggests that microRNAs play critical roles in chondrogenic differentiation of mesenchymal stem cels. OBJECTIVE:To observe the chondrogenic effect of human bone marrow mesenchymal stem cels transfected with lentiviral vectors bearing miR-221-3p/222-3p inhibition, thereby provding new strategies for cartilage injury. METHODS: miRNA microarray technology was applied to detect microRNAs expression profiles at three different stages of chondrogenic differentiation induction after transforming growth factor-β3 treatment and verified by real-time fluorescence quantitative PCR (RT-qPCR). Human bone marrow mesenchymal stem cels were infected with lentivirus bearing miR-221-3p/222-3p inhibition. After co-suppressing the expression of miR-221/222-3p, cel counting kit-8 was used to determine the cel proliferation, the differentiation of bone marrow mesenchymal stem cels towards chondrocytes was verified by type II colagen protein expression through immunohistochemistry and glycosaminoglycan accumulation was also elevated by sarranine O staining. RT-PCR was used to detect type II colagen and aggrecan mRNA expression at 21 days of chondrogenic induction. RESULTS AND CONCLUSION: The expression of miR-221-3p/222-3p was inhibited after Lv-miR221-3p/222-3p inhibition co-transfected into bone marrow mesenchymal stem cels. microRNA microarray and RT-qPCR results showed that the expression of miR-221-3p/222-3p was declined significantly at the anaphase of chondrogenic differentiation. The expression levels of chondrogenic markers, Aggrecan and type II colagen were significantly increased in the miR-221-3p/222-3p inhibition group and cel proliferation was also inhibited significantly compared with non-transduced cels or transduced with the empty 18. Modelling the biogeochemical cycle of silicon in soils using the reactive transport code MIN3P Science.gov (United States) Gerard, F.; Mayer, K. U.; Hodson, M. J.; Meunier, J. 2006-12-01 We investigated the biogeochemical cycling of Si in an acidic brown soil covered by a coniferous forest (Douglas fir) based on a comprehensive data set and reactive transport modelling. Both published and original data enable us to make up a conceptual model on which the development of a numerical model is based. We modified the reactive transport code MIN3P, which solves thermodynamic and kinetic reactions coupled with vadose zone flow and solute transport. Simulations were performed for a one-dimensional heterogeneous soil profile and were constrained by observed data including daily soil temperature, plant transpiration, throughfall, and dissolved Si in solutions collected beneath the organic layer. Reactive transport modelling was first used to test the validity of the hypothesis that a dynamic balance between Si uptake by plants and release by weathering controls aqueous Si-concentrations. We were able to calibrate the model quite accurately by stepwise adjustment of the relevant parameters. The capability of the model to predict Si-concentrations was good. Mass balance calculations indicate that only 40% of the biogeochemical cycle of Si is controlled by weathering and that about 60% of Si-cycling is related to biological processes (i.e. Si uptake by plants and dissolution of biogenic Si). Such a large contribution of biological processes was not anticipated considering the temperate climate regime, but may be explained by the high biomass productivity of the planted coniferous species. The large contribution of passive Si-uptake by vegetation permits the conservation of seasonal concentration variations caused by temperature-induced weathering, although the modelling suggests that the latter process was of lesser importance relative to biological Si-cycling. 19. Feline Immunodeficiency Virus Vif N-Terminal Residues Selectively Counteract Feline APOBEC3s. Science.gov (United States) Gu, Qinyong; Zhang, Zeli; Cano Ortiz, Lucía; Franco, Ana Cláudia; Häussinger, Dieter; Münk, Carsten 2016-12-01 Feline immunodeficiency virus (FIV) Vif protein counteracts feline APOBEC3s (FcaA3s) restriction factors by inducing their proteasomal degradation. The functional domains in FIV Vif for interaction with FcaA3s are poorly understood. Here, we have identified several motifs in FIV Vif that are important for selective degradation of different FcaA3s. Cats (Felis catus) express three types of A3s: single-domain A3Z2, single-domain A3Z3, and double-domain A3Z2Z3. We proposed that FIV Vif would selectively interact with the Z2 and the Z3 A3s. Indeed, we identified two N-terminal Vif motifs (12LF13 and 18GG19) that specifically interacted with the FcaA3Z2 protein but not with A3Z3. In contrast, the exclusive degradation of FcaA3Z3 was regulated by a region of three residues (M24, L25, and I27). Only a FIV Vif carrying a combination of mutations from both interaction sites lost the capacity to degrade and counteract FcaA3Z2Z3. However, alterations in the specific A3s interaction sites did not affect the cellular localization of the FIV Vif protein and binding to feline A3s. Pulldown experiments demonstrated that the A3 binding region localized to FIV Vif residues 50 to 80, outside the specific A3 interaction domain. Finally, we found that the Vif sites specific to individual A3s are conserved in several FIV lineages of domestic cat and nondomestic cats, while being absent in the FIV Vif of pumas. Our data support a complex model of multiple Vif-A3 interactions in which the specific region for selective A3 counteraction is discrete from a general A3 binding domain. 20. PRR performance of Cu- and CuBr-vapor lasers Science.gov (United States) Fedorov, V. F.; Evtushenko, Gennadiy S.; Klimkin, Vladimir M.; Polunin, Yu. P.; Soldatov, Anatoly N.; Sukhanov, Viktor B. 1998-06-01 Results obtained from comparative analysis of the pulse repetition rate performance of Cu- and CuBr-vapor lasers operated at high pump pulse repetitions (approximately 100 kHz) are reported. For a CuBr laser with a 8 mm diameter discharge tube the laser pulse repetition rate as high as 270 kHz was realized. 1. Cu Diffusion in Co/Cu/TiN Films for Cu Metallization Institute of Scientific and Technical Information of China (English) Xiuhua CHEN; Xinghui WU; Jinzhong XIANG; Zhenlai ZHOU; Heyun ZHAO; Liqiang CHEN 2006-01-01 Some information on how to use in-situ determined diffusion coefficient of Cu to make barrier layer of Cu metallization in ultra large scale integrations (ULSIs) was provided. Diffusion coefficients of Cu in Co at low temperature were determined to analyze Cu migration to Co surface layer. The diffusion depths were analyzed using X-ray photoelectron spectroscopy (XPS) depth profile to investigate the diffusion effect of Cu in Co at different temperatures. The possible pretreatment temperature and time of barrier layer can be predicted according to the diffusion coefficients of Cu in Co. 2. External lipid PI3P mediates entry of eukaryotic pathogen effectors into plant and animal host cells. Science.gov (United States) Kale, Shiv D; Gu, Biao; Capelluto, Daniel G S; Dou, Daolong; Feldman, Emily; Rumore, Amanda; Arredondo, Felipe D; Hanlon, Regina; Fudal, Isabelle; Rouxel, Thierry; Lawrence, Christopher B; Shan, Weixing; Tyler, Brett M 2010-07-23 Pathogens of plants and animals produce effector proteins that are transferred into the cytoplasm of host cells to suppress host defenses. One type of plant pathogens, oomycetes, produces effector proteins with N-terminal RXLR and dEER motifs that enable entry into host cells. We show here that effectors of another pathogen type, fungi, contain functional variants of the RXLR motif, and that the oomycete and fungal RXLR motifs enable binding to the phospholipid, phosphatidylinositol-3-phosphate (PI3P). We find that PI3P is abundant on the outer surface of plant cell plasma membranes and, furthermore, on some animal cells. All effectors could also enter human cells, suggesting that PI3P-mediated effector entry may be very widespread in plant, animal and human pathogenesis. Entry into both plant and animal cells involves lipid raft-mediated endocytosis. Blocking PI3P binding inhibited effector entry, suggesting new therapeutic avenues. 3. Theoretical study on the two-band degenerate-gaps superconductors: Application to SrPt3P Science.gov (United States) Huang, Hai; Hou, Li-Chao; Zhao, Bin-Peng 2016-09-01 We study the magnetic properties of two-band degenerate-gaps superconductors with two-band isotropic Ginzburg-Landau theory. The exact solutions of upper critical field and London penetration depth are obtained, and the calculations reproduce the experimental data of the recently observed superconducting crystal SrPt3P in a broad temperature range. It directly underlies that SrPt3P is a multi-band superconductor with equal gaps in two Fermi surface sheets. 4. The Involvement of miR-29b-3p in Arterial Calcification by Targeting Matrix Metalloproteinase-2 Science.gov (United States) Jiang, Wenhong; Zhang, Zhanman; Yang, Han; Lin, Qiuning; Han, Chuangye 2017-01-01 Vascular calcification is a risk predictor and common pathological change in cardiovascular diseases that are associated with elastin degradation and phenotypic transformation of vascular smooth muscle cells via gelatinase matrix metalloproteinase-2 (MMP2). However, the mechanisms involved in this process remain unclear. In this study, we investigated the relationships between miR-29b-3p and MMP2, to confirm miR-29b-3p-mediated MMP2 expression at the posttranscriptional level in arterial calcification. In male Sprague Dawley rats, arterial calcification was induced by subcutaneous injection of a toxic dose of cholecalciferol. In vivo, the quantitative real-time polymerase chain reaction (qRT-PCR) showed that MMP2 expression was upregulated in calcified arterial tissues, and miR-29b-3p expression was downregulated. There was a negative correlation between MMP2 mRNA expression and miR-29b-3p levels (P = 0.0014, R2 = 0.481). Western blotting showed that MMP2 expression was significantly increased in rats treated with cholecalciferol. In vitro, overexpression of miR-29b-3p led to decreased MMP2 expression in rat vascular smooth muscle cells, while downregulation of miR-29b-3p expression led to increased MMP2 expression. Moreover, the luciferase reporter assay confirmed that MMP2 is the direct target of miR-29b-3p. Together, our results demonstrated that a role of miR-29b-3p in vascular calcification involves targeting MMP2. PMID:28164126 5. The Involvement of miR-29b-3p in Arterial Calcification by Targeting Matrix Metalloproteinase-2 Directory of Open Access Journals (Sweden) Wenhong Jiang 2017-01-01 Full Text Available Vascular calcification is a risk predictor and common pathological change in cardiovascular diseases that are associated with elastin degradation and phenotypic transformation of vascular smooth muscle cells via gelatinase matrix metalloproteinase-2 (MMP2. However, the mechanisms involved in this process remain unclear. In this study, we investigated the relationships between miR-29b-3p and MMP2, to confirm miR-29b-3p-mediated MMP2 expression at the posttranscriptional level in arterial calcification. In male Sprague Dawley rats, arterial calcification was induced by subcutaneous injection of a toxic dose of cholecalciferol. In vivo, the quantitative real-time polymerase chain reaction (qRT-PCR showed that MMP2 expression was upregulated in calcified arterial tissues, and miR-29b-3p expression was downregulated. There was a negative correlation between MMP2 mRNA expression and miR-29b-3p levels (P=0.0014, R2=0.481. Western blotting showed that MMP2 expression was significantly increased in rats treated with cholecalciferol. In vitro, overexpression of miR-29b-3p led to decreased MMP2 expression in rat vascular smooth muscle cells, while downregulation of miR-29b-3p expression led to increased MMP2 expression. Moreover, the luciferase reporter assay confirmed that MMP2 is the direct target of miR-29b-3p. Together, our results demonstrated that a role of miR-29b-3p in vascular calcification involves targeting MMP2. 6. Ginkgolide B Inhibits Human Bladder Cancer Cell Migration and Invasion Through MicroRNA-223-3p Directory of Open Access Journals (Sweden) Yi Zhi 2016-10-01 Full Text Available Background/Aims: Ginkgolide B (GB is currently used as an anticancer drug for treatment of some malignant cancers. However, whether it may have therapeutic effects on bladder cancer remains unknown. Here, we studied the effects of GB on bladder cancer cells. Methods: Bladder cells were treated with different doses of GB, and the effects on ZEB1 and microRNA-223-3p (miR-223-3p were analyzed by RT-qPCR and/or Western blot. Prediction of a regulatory relationship between miR-93 and 3'-UTR of Beclin-1 mRNA was performed by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Results: We found that GB dose-dependently decreased ZEB1 protein, but not mRNA, in bladder cancer cells, resulting in suppression of cell invasion. Moreover, in bladder cancer cells, GB dose-dependently decreased the levels of miR-223-3p, which suppressed the protein translation of ZEB1 through binding to 3'-UTR of ZEB1 mRNA. Overexpression of miR-223-3p decreased ZEB1 protein, while depletion of miR-223-3p increased ZEB1 protein in bladder cancer cells. Conclusion: GB inhibits bladder cancer cell invasiveness through suppressing ZEB1 protein translation via upregulating miR-223-3p. 7. MiR-525-3p enhances the migration and invasion of liver cancer cells by downregulating ZNF395. Directory of Open Access Journals (Sweden) Fei Pang Full Text Available Liver cancer is one of leading causes of cancer-related deaths. A deeper mechanistic understanding of liver cancer could lead to the development of more effective therapeutic strategies. In our previous work, we screened 646 miRNAs and identified 11 that regulate liver cancer cell migration. The current study shows that miR-525-3p is frequently up-regulated in liver cancer tissues, and enhanced expression of miR-525-3p can promote liver cancer cell migration and invasion. Zinc finger protein 395 (ZNF395 is the direct functional target gene for miR-525-3p, and it is frequently down-regulated in liver cancer tissues. High expression of ZNF395 can significantly inhibit while knockdown of ZNF395 expression can markedly enhance the migration and invasion of liver cancer cells, suggesting that ZNF395 suppresses metastasis in liver cancer. Down-regulation of ZNF395 can mediate miR-525-3p induced liver cancer cell migration and invasion. In conclusion, miR-525-3p promotes liver cancer cell migration and invasion by directly targeting ZNF395, and the fact that miR-525-3p and ZNF395 both play important roles in liver cancer progression makes them potential therapeutic targets. 8. Chemical preparation, kinetics of thermal behavior and infrared studies of Pb3(P3O92.3H2O and Cd3(P3O92.14H2O Directory of Open Access Journals (Sweden) Said Belaaouad 2013-05-01 Full Text Available Chemical preparation, thermal behavior, kinetic and IR studies are given for the cyclotriphosphates Pb3(P3O92.3H2O and Cd3(P3O92.14H2O. The later cyclotriphosphates have never been studied except their crystallographic characterization and are stable in the conditions of temperature and pressure of our laboratory until 343K. The final products of the dehydration and calcination of Pb3(P3O92.3H2O and Cd3(P3O92.14H2O, under atmospheric pressure, are respectively their long chain polyphosphates, [Pb(PO32]∞ and β[Cd(PO32]∞. The intermediate product of the dehydration of Cd3(P3O92.14H2O, under atmospheric pressure, is its long chain polyphosphate form α, α[Cd(PO32]. [Pb(PO32]∞ and β[Cd(PO32]∞ are stable until their melting points at respectively 946K and 1153K. Two different methods, Ozawa and KAS have been selected in order to study the kinetics of thermal behavior of the cyclotriphosphates Pb3(P3O92.3H2O and Cd3(P3O92.14H2O for the first time. The kinetic and thermodynamic features of the dehydration, of the cited cyclotriphosphates, were determined and discussed on the basis of their crystalline structure. [Pb(PO32]∞, α[Cd(PO32] and β[Cd(PO32] have many applications in industry such as corrosion inhibitors. 9. Relationship between miRNA-338-3p expression and progression and prognosis of human colorectal carcinoma Institute of Scientific and Technical Information of China (English) Sun Kai; Su Guiyuan; Deng Haijun; Dong Jingqing; Lei Shangtong; Li Guoxin 2014-01-01 Background miR-338-3p is a recently discovered miRNA and is involved in cell differentiation.However,few data are yet available on the aberrant expression of miR-338-3p in human colorectal carcinoma (CRC).This work aimed to investigate the relationship between miR-338-3p expression pattern and clinicopathological features of human CRC and the possible regulative mechanisms.Methods The 40 CRC,adjacent nontumorous tissues and 2 human CRC-derived cell lines (SW-480 and SW-620) were collected,respectively,and the total RNA and protein were isolated routinely.The miR-338-3p expression pattern was detected by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Northern blotting.Smoothened (SMO,possible target of miR-338-3p) mRNA and corresponding protein expression pattern were detected by semiquantitative RT-PCR and Western blotting.miR-338-3p expression patterns were compared between nontumor mucosa and CRC samples,graded by progression-related factors.Disease outcome was calculated by Kaplan-Meier survival analysis to determine whether miR-338-3p was related to disease-free survival (DFS) and overall survival (OS) of patients.Moreover,SMO 3'-UTR fragment was PCR amplified from genome DNA of human colon and inserted into a luciferase reporter plasmid.The luciferase reporter plasmid construct was then transfected into CRC cells together with pre-miR-338-3p or anti-miR-338-3p and the luciferase activity in the transfected cells was detected.Results The expression of miR-338-3p was significantly downregulated in CRCs than those in the adjacent nontumorous tissues,and the value was negatively related to advanced TNM stage and local invasion (P ＜0.01).Furthermore,miR338-3p value was decreased markedly in SW-620 cell line relative to SW-480 (P ＜0.01).Low expression of miR-338-3p was associated with unfavorable outcome in DFS but not in OS independent of clinical covariates.Moreover,RT-PCR and Western blotting analysis demonstrated that there was no 10. About Region 3's Laboratory and Field Services at EPA's Environmental Science Center Science.gov (United States) Mission & contact information for EPA Region 3's Laboratory and Field Services located at EPA's Environmental Science Center: the Office of Analytical Services and Quality Assurance & Field Inspection Program 11. Reactions of N+ (3P) ions with H2 and HD molecules at low temperatures Science.gov (United States) Grozdanov, Tasko P.; McCarroll, Ronald; Roueff, Evelyne 2016-05-01 formation of ND+. The calculated value is consistent with the available experimental data. Conclusions: The present results allow for the determination of reaction rate coefficients for any given distribution of specific fine structure and rotational state populations of the reactants. In interstellar conditions, where N+ is in its 3P0 state and para- and ortho-H2 respectively in J = 0 and J = 1. Our results enable a study of the influence of the ortho/para evolution of molecular hydrogen on the formation of nitrogen compounds. 12. A Study to Interpret the Biological Significance of Behavior Associated with 3S Experimental Sonar Exposures Science.gov (United States) 2014-09-30 Behavior Associated with 3S Experimental Sonar Exposures Patrick Miller Sea Mammal Research Unit Scottish Oceans Institute School of Biology...to investigate behavioral reactions of cetaceans to naval sonar and various control sounds, and the sound exposures required to elicit responses, in...order to establish safety limits for sonar operations. 3S project efforts have been focused upon informing the ‘exposure-effect assessment 13. Establishing Forest Landscape Resources Information System Based on 3S Technique Institute of Scientific and Technical Information of China (English) 2003-01-01 The application of Geographic Information System(GIS), Remote Sensing(RS) and Global Position System(GPS) in the research of forest landscape is outlined in this paper. The integration of the 3S technique is also described. In the 3S system, RS is used to obtain the various information of forest landscape. GPS is used to obtain the ground data of orientation and guide the people to the different places. And GIS is used in information management and processing. 14. Optically Forbidden Excitations of 3s Electron of Argon by Fast Electron Impact Institute of Scientific and Technical Information of China (English) 朱林繁; 成华东; 刘小井; 田鹏; 苑震生; 李文斌; 徐克尊 2003-01-01 The electron energy loss spectrum of argon in the energy region of 24.5-30.5eV was measured at 2.5 keV impact energy. The line profile parameters of the optically forbidden excitations of 3s-1ns (n = 4-6) and 3s-1nd (n = 3-7) of argon, I.e.,Eγ,Г,q and p,were determined. 15. miR-4782-3p Inhibited Non-Small Cell Lung Cancer growth via USP14 Directory of Open Access Journals (Sweden) Ning Wu 2014-02-01 Full Text Available Background: Lung cancer is the leading cause of cancer-related mortality worldwide, with near 1.4 million deaths each year. NSCLC accounts for nearly 85% of all case of lung cancer. MiRNAs play important roles in regulation of gene expression at the post-transcriptional level. MiRNAs profiles may predict prognosis and disease recurrence in early-stage NSCLC. Our previous study proved that over-expression of ubiquitin specific peptidase 14 (USP14, a deubiquitinating enzyme, was associated with favorable prognosis in NSCLC patients and promoted tumor cells proliferation. Here, we tried to identify which miRNAs targeted USP14, and the roles of these miRNAs in NSCLC. Methods: MiR-4782-3p and its potential targeted genes were identified by bioinformatics algorithm. Dual luciferase reporter assay system was used to analyze the interaction between miR-4782-3p and targeted genes. Cell proliferation was assayed by MTT and BdU assay. MiRNAs and mRNA expression were assayed by qRT-PCR. USP14 protein level was assayed by Western blot. The role of miR-4782-3p in patients survival was revealed by Kaplan-Meier plot of overall survival. Results: Up-expression of miR-4782-3p in NSCLC cells decreased the USP14 expression. Down-expression of miR-4782-3p increased USP14 expression. In NSCLC specimen, Negative correlation between USP14 mRNA level and miR-4782-3p level was identified. Higher miR-4782-3p expression is associated with longer survival. USP14, ZEB2, XIAP overexpression reversed the inhibitory effect of miR-4782-3p. Conclusions: High expression of miR-4782-3p was associated with favorable prognosis in NSCLC patients. MiR-4782-3p inhibited cell proliferation in NSCLC by targeting USP14, ZEB2 and XIAP. 16. R loops are linked to histone H3 S10 phosphorylation and chromatin condensation. Science.gov (United States) Castellano-Pozo, Maikel; Santos-Pereira, José M; Rondón, Ana G; Barroso, Sonia; Andújar, Eloisa; Pérez-Alegre, Mónica; García-Muse, Tatiana; Aguilera, Andrés 2013-11-21 R loops are transcription byproducts that constitute a threat to genome integrity. Here we show that R loops are tightly linked to histone H3 S10 phosphorylation (H3S10P), a mark of chromatin condensation. Chromatin immunoprecipitation (ChIP)-on-chip (ChIP-chip) analyses reveal H3S10P accumulation at centromeres, pericentromeric chromatin, and a large number of active open reading frames (ORFs) in R-loop-accumulating yeast cells, better observed in G1. Histone H3S10 plays a key role in maintaining genome stability, as scored by ectopic recombination and plasmid loss, Rad52 foci, and Rad53 checkpoint activation. H3S10P coincides with the presence of DNA-RNA hybrids, is suppressed by ribonuclease H overexpression, and causes reduced accessibility of restriction endonucleases, implying a tight connection between R loops, H3S10P, and chromatin compaction. Such histone modifications were also observed in R-loop-accumulating Caenorhabditis elegans and HeLa cells. We therefore provide a role of RNA in chromatin structure essential to understand how R loops modulate genome dynamics. 17. Visual Display of 5p-arm and 3p-arm miRNA Expression with a Mobile Application Directory of Open Access Journals (Sweden) Chao-Yu Pan 2017-01-01 Full Text Available MicroRNAs (miRNAs play important roles in human cancers. In previous studies, we have demonstrated that both 5p-arm and 3p-arm of mature miRNAs could be expressed from the same precursor and we further interrogated the 5p-arm and 3p-arm miRNA expression with a comprehensive arm feature annotation list. To assist biologists to visualize the differential 5p-arm and 3p-arm miRNA expression patterns, we utilized a user-friendly mobile App to display. The Cancer Genome Atlas (TCGA miRNA-Seq expression information. We have collected over 4,500 miRNA-Seq datasets from 15 TCGA cancer types and further processed them with the 5p-arm and 3p-arm annotation analysis pipeline. In order to be displayed with the RNA-Seq Viewer App, annotated 5p-arm and 3p-arm miRNA expression information and miRNA gene loci information were converted into SQLite tables. In this distinct application, for any given miRNA gene, 5p-arm miRNA is illustrated on the top of chromosome ideogram and 3p-arm miRNA is illustrated on the bottom of chromosome ideogram. Users can then easily interrogate the differentially 5p-arm/3p-arm expressed miRNAs with their mobile devices. This study demonstrates the feasibility and utility of RNA-Seq Viewer App in addition to mRNA-Seq data visualization. 18. Luteolin Inhibits Ischemia/Reperfusion-Induced Myocardial Injury in Rats via Downregulation of microRNA-208b-3p. Directory of Open Access Journals (Sweden) Chen Bian Full Text Available Luteolin (LUT, a kind of flavonoid which is extracted from a variety of diets, has been reported to convey protective effects of various diseases. Recent researches have suggested that LUT can carry out cardioprotective effects during ischemia/reperfusion (I/R. However, there have no reports on whether LUT can exert protective effects against myocardial I/R injury through the actions of specific microRNAs (miRs. The purpose of this study was to determine which miRs and target genes LUT exerted such function through.Expression of various miRs in perfused rat hearts was detected using a gene chip. Target genes were predicted with TargetScan, MiRDB and MiRanda. Anoxia/reoxygenation was used to simulate I/R. Cells were transfected by miR-208b-3p mimic, inhibitor and small interfering RNA of Ets1 (avian erythroblastosis virus E26 (v ets oncogene homolog 1. MiR-208b-3p and Ets1 mRNA were quantified by real-time quantitative polymerase chain reaction. The percentage of apoptotic cells was detected by annexin V-fluorescein isothiocyanate/propidium iodide dyeing and flow cytometry. The protein expression levels of cleaved caspase-3, Bcl-2, Bax, and Ets1 were examined by western blot analysis. A luciferase reporter assay was used to verify the combination between miR-208b-3p and the 3'-untranslated region of Ets1.LUT pretreatment reduced miR-208b-3p expression in myocardial tissue, as compared to the I/R group. And LUT decreased miR-208b-3p expression and apoptosis caused by I/R. However, overexpression of miR-208b-3p further aggravated the changes caused by I/R and blocked all the effects of LUT. Knockdown of miR-208b-3p expression also attenuated apoptosis, while knockdown of Ets1 promoted apoptosis. Further, the luciferase reporter assay showed that miR-208b-3p could inhibit Ets1 expression.LUT pretreatment conveys anti-apoptotic effects after myocardial I/R injury by decreasing miR-208b-3p and increasing Ets1 expression levels. 19. Angle-integrated photoemission studies of ruthocuprate Eu_2-xCe_xRuSr_2Cu_2O_10, Gd_2RuSr_2Cu_2O_10 and Eu_1.5Nb_1-xRu_xCu_2O_10 systems Science.gov (United States) Frazer, B.; Hirai, Y.; Rast, Simon; Felner, I.; Asaf, U.; Onellion, M. 2000-03-01 We report on both resonant photoemission and fixed photon energy studies of the conduction band and core levels for several ruthocuprate systems. The pure Ru-containing compounds exhibit ferromagnetic order, with metallic behavior and superconductivity depending on the rare earth and oxygen content. We report on as-prepared, hydrogen loaded, and oxygen-annealed polycrystalline samples. The Nb-Ru series changes from purely superconducting to both ferromagnetic and superconducting (Ru). The resonant photoemission measurements of the conduction band across the Ru4p, Cu3p, and Eu4d core levels allow us to determine the location and contribution of Ru, Cu and rare earth related states in the conduction band. The O1s, Cu2p, Ru3p, Ce4d, and Gd4d and several Nb core levels allow us to determine the valence of Ru and Nb, and the metallicity of the Ru/Nb, rare earth, and CuO2 planes. 20. Propofol Inhibits Neurogenesis of Rat Neural Stem Cells by Upregulating MicroRNA-141-3p. Science.gov (United States) Jiang, Qiliang; Wang, Yingwei; Shi, Xueyin 2017-02-01 Prolonged or high-dose exposure to anesthetics, such as propofol, can cause brain cell degeneration and subsequent long-term learning or memory deficits, particularly in the developing brain. However, the cellular and molecular mechanisms underlying the deleterious effects of propofol at certain stages of development remain unclear. In this study we found that propofol inhibited the proliferation, neuronal differentiation, and migration of neural stem cells (NSCs) while upregulating miR-141-3p. Silencing of miR-141-3p abrogated the effects of propofol on NSC neurogenesis. Propofol treatment downregulated IGF2BP2, a direct target of miR-141-3p, whereas overexpression of IGF2BP2 attenuated the effects of propofol and miR-141-3p on NSC neurogenesis. In short, propofol inhibits NSC neurogenesis through a mechanism involving the miR-141-3p/IGF2BP2 axis. Our results may provide a potential approach for preventing the neurodegenerative effects of propofol in the developing brain. 1. miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110. Science.gov (United States) Bijnsdorp, Irene V; Hodzic, Jasmina; Lagerweij, Tonny; Westerman, Bart; Krijgsman, Oscar; Broeke, Jurjen; Verweij, Frederik; Nilsson, R Jonas A; Rozendaal, Lawrence; van Beusechem, Victor W; van Moorselaar, Jeroen A; Wurdinger, Thomas; Geldof, Albert A 2016-03-29 The centrosome plays a key role in cancer invasion and metastasis. However, it is unclear how abnormal centrosome numbers are regulated when prostate cancer (PCa) cells become metastatic. CP110 was previously described for its contribution of centrosome amplification (CA) and early development of aggressive cell behaviour. However its regulation in metastatic cells remains unclear. Here we identified miR-129-3p as a novel metastatic microRNA. CP110 was identified as its target protein. In PCa cells that have metastatic capacity, CP110 expression was repressed by miR-129-3p. High miR-129-3p expression levels increased cell invasion, while increasing CP110 levels decreased cell invasion. Overexpression of CP110 in metastatic PCa cells resulted in a decrease in the number of metastasis. In tissues of PCa patients, low CP110 and high miR-129-3p expression levels correlated with metastasis, but not with the expression of genes related to EMT. Furthermore, overexpression of CP110 in metastatic PCa cells resulted in excessive-CA (E-CA), and a change in F-actin distribution which is in agreement with their reduced metastatic capacity. Our data demonstrate that miR-129-3p functions as a CA gatekeeper in metastatic PCa cells by maintaining pro-metastatic centrosome amplification (CA) and preventing anti-metastatic E-CA. 2. Development of a chromosomally integrated metabolite-inducible Leu3p-alpha-IPM "off-on" gene switch. Directory of Open Access Journals (Sweden) Maria Poulou Full Text Available BACKGROUND: Present technology uses mostly chimeric proteins as regulators and hormones or antibiotics as signals to induce spatial and temporal gene expression. METHODOLOGY/PRINCIPAL FINDINGS: Here, we show that a chromosomally integrated yeast 'Leu3p-alpha-IotaRhoMu' system constitutes a ligand-inducible regulatory "off-on" genetic switch with an extensively dynamic action area. We find that Leu3p acts as an active transcriptional repressor in the absence and as an activator in the presence of alpha-isopropylmalate (alpha-IotaRhoMu in primary fibroblasts isolated from double transgenic mouse embryos bearing ubiquitously expressing Leu3p and a Leu3p regulated GFP reporter. In the absence of the branched amino acid biosynthetic pathway in animals, metabolically stable alpha-IPM presents an EC(50 equal to 0.8837 mM and fast "OFF-ON" kinetics (t(50ON = 43 min, t(50OFF = 2.18 h, it enters the cells via passive diffusion, while it is non-toxic to mammalian cells and to fertilized mouse eggs cultured ex vivo. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that the 'Leu3p-alpha-IotaRhoMu' constitutes a simpler and safer system for inducible gene expression in biomedical applications. 3. miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesis. Science.gov (United States) Tariq, Kaleem; Metzendorf, Christoph; Peng, Wei; Sohail, Summar; Zhang, Hongyu 2016-03-02 Genetics-enhanced sterile insect techniques (SIT) are promising novel approaches to control Bactrocera dorsalis, the most destructive horticultural pest in East Asia and the Pacific region. To identify novel genetic agents to alter male fertility of B. dorsalis, previous studies investigated miRNA expression in testes of B. dorsalis. One miRNA, miR-8-3p was predicted to bind the 3'UTR of putative B. dorsalis mitoferrin (bmfrn). The ortholog of bmfrn in D. melanogaster is essential for male fertility. Here we show that bmfrn has all conserved amino acid residues of known mitoferrins and is most abundantly expressed in B. dorsalis testes, making miR-8-3p and mitoferrin candidates for genetics-enhanced SIT. Furthermore, using a dual-luciferase reporter system, we show in HeLa cells that miR-8-3p interacts with the 3'UTR of bmfrn. Dietary treatments of adult male flies with miR-8-3p mimic, antagomiR, or bmfrn dsRNA, altered mitoferrin expression in the testes and resulted in reduced male reproductive capacity due to reduced numbers and viability of spermatozoa. We show for the first time that a mitoferrin is regulated by a miRNA and we demonstrate miR-8-3p as well as bmfrn dsRNA to be promising novel agents that could be used for genetics-enhanced SIT. 4. Combination of MiR-103a-3p and Mesothelin Improves the Biomarker Performance of Malignant Mesothelioma Diagnosis Science.gov (United States) Bryk, Oleksandr; Raiko, Irina; Pesch, Beate; Kollmeier, Jens; Bauer, Torsten T.; Brüning, Thomas 2014-01-01 Background For the detection of malignant mesothelioma no single biomarker with reasonable sensitivity and specificity has been described so far. Mesothelin, the most prominent blood-based biomarker, is characterized by high specificity but low sensitivity. It might be reasonable to combine biomarkers of different molecular classes in order to improve the overall performance. The aim of this study was to assess the performance of the combination of mesothelin and miR-103a-3p as blood-based biomarker for mesothelioma. Methods/Principal Findings Mesothelin concentration in plasma and miR-103a-3p levels in the cellular blood fraction were analyzed in 43 male mesothelioma patients and 52 male controls formerly exposed to asbestos. For the discrimination of epithelioid and biphasic mesothelioma from asbestos-exposed controls mesothelin and miR-103a-3p showed 74% and 89% sensitivity and 85% and 63% specificity, respectively. For the combination of mesothelin and miR-103a-3p a sensitivity of 95% and a specificity of 81% were calculated. Conclusions/Significance The results of this study show that the combination of mesothelin and miR-103a-3p improves the diagnostic performance of individual blood-based biomarker to detect malignant mesothelioma. The obtained results indicate that the use of biomarkers of different molecular classes might be a reasonable approach to assemble a biomarker panel. PMID:25469901 5. Combination of MiR-103a-3p and mesothelin improves the biomarker performance of malignant mesothelioma diagnosis. Directory of Open Access Journals (Sweden) Daniel G Weber Full Text Available For the detection of malignant mesothelioma no single biomarker with reasonable sensitivity and specificity has been described so far. Mesothelin, the most prominent blood-based biomarker, is characterized by high specificity but low sensitivity. It might be reasonable to combine biomarkers of different molecular classes in order to improve the overall performance. The aim of this study was to assess the performance of the combination of mesothelin and miR-103a-3p as blood-based biomarker for mesothelioma.Mesothelin concentration in plasma and miR-103a-3p levels in the cellular blood fraction were analyzed in 43 male mesothelioma patients and 52 male controls formerly exposed to asbestos. For the discrimination of epithelioid and biphasic mesothelioma from asbestos-exposed controls mesothelin and miR-103a-3p showed 74% and 89% sensitivity and 85% and 63% specificity, respectively. For the combination of mesothelin and miR-103a-3p a sensitivity of 95% and a specificity of 81% were calculated.The results of this study show that the combination of mesothelin and miR-103a-3p improves the diagnostic performance of individual blood-based biomarker to detect malignant mesothelioma. The obtained results indicate that the use of biomarkers of different molecular classes might be a reasonable approach to assemble a biomarker panel. 6. Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion. Science.gov (United States) Shen, Shu; Tobery, Cynthia E; Rose, Mark D 2009-05-01 Nuclear membrane fusion is the last step in the mating pathway of the yeast Saccharomyces cerevisiae. We adapted a bioinformatics approach to identify putative pheromone-induced membrane proteins potentially required for nuclear membrane fusion. One protein, Prm3p, was found to be required for nuclear membrane fusion; disruption of PRM3 caused a strong bilateral defect, in which nuclear congression was completed but fusion did not occur. Prm3p was localized to the nuclear envelope in pheromone-responding cells, with significant colocalization with the spindle pole body in zygotes. A previous report, using a truncated protein, claimed that Prm3p is localized to the inner nuclear envelope. Based on biochemistry, immunoelectron microscopy and live cell microscopy, we find that functional Prm3p is a peripheral membrane protein exposed on the cytoplasmic face of the outer nuclear envelope. In support of this, mutations in a putative nuclear localization sequence had no effect on full-length protein function or localization. In contrast, point mutations and deletions in the highly conserved hydrophobic carboxy-terminal domain disrupted both protein function and localization. Genetic analysis, colocalization, and biochemical experiments indicate that Prm3p interacts directly with Kar5p, suggesting that nuclear membrane fusion is mediated by a protein complex. 7. Final state interaction observed in M{sub 2,3}VV Auger profile of Cu(110) Energy Technology Data Exchange (ETDEWEB) Nakajima, H; Songsiriritthigul, P [Synchrotron Light Research Institute, 111 University Avenue, Muang District, Nakhon Ratchasima 30000 (Thailand); Buddhakala, M [Department of Physics, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, 39 Muh 1, Rangsit-Nakhonnayok Road Klong Hok, Thanyaburi District, Pathumthani 12110 (Thailand); Chumpolkulwong, S [Department of Physics, Faculty of Science, Udon Thani Rajabhat University, 64 Thahan Road, Muang District, Udon Thani 41000 (Thailand); Kakizaki, A [Institute for Solid State Physics, The University of Tokyo, Kashiwa, Chiba 277-8581 (Japan)], E-mail: hiden@nsrc.or.th 2009-02-04 We have measured the M{sub 2,3}VV Auger spectra of Cu(110) and studied the final state interaction following the Cu 3p core electron excitation. We have observed that the kinetic energy of the M{sub 2,3}VV Auger electron shifts to an energy higher than that of the normal Auger electrons near the Cu 3p threshold, and it converges to the constant kinetic energy of the normal Auger electrons as the excitation energy increases above the Cu 3p threshold. In the excitation energy dependence of the kinetic energies of the M{sub 2,3}VV Auger electrons, we observed step features at the excitation energies corresponding to the 3p core electron excitations to the L{sub 1} and X{sub 1} van Hove singularities in the valence states. The kinetic energy shifts of the M{sub 2,3}VV Auger electrons are reasonably understood by considering the localization of the two-hole Auger final state and the hybridization between Cu 3d states and other valence states. 8. Regulatory role of the sequences downstream from nodD3 P1 promoter of Rhizobium meliloti Institute of Scientific and Technical Information of China (English) 2000-01-01 The 660 bp region between nodD3 P1 promoter and the following coding region of Rhizobium meliloti has been studied.This region is designated "downstream sequences".It consists of two potential open reading frames,ORF1 and ORF2.Studies on the role of the downstream sequences on the activity of nodD3 P1 with nod D3(P1)-lacZ fusion show that deletion of the sequences containing ORF2 causes the increase of the activity of the fusion; on the contrary,addition of extra copies of ORF2 markedly decreases the activity of the fusion.These results indicate that the product of ORF2 plays a negative role in the expression of nod D3. 9. Biomimetic-Inspired Infrared Sensors from Zn3P2 Microwires: Study of Their Photoconductivity and Infrared Spectrum Properties Directory of Open Access Journals (Sweden) M. Israelowitz 2014-01-01 Full Text Available The fire beetle, Melanophila acuminata (Coleoptera: Buprestidae, senses infrared radiation at wavelengths of 3 and 10–25 microns via specialized protein-containing sensilla. Although the protein denatures outside of a biological system, this detection mechanism has inspired our bottom-up approach to produce single zinc phosphide microwires via vapour transport for IR sensing. The Zn3P2 microwires were immobilized and electrical contact was made by dielectrophoresis. Photoconductivity measurements have been extended to the near IR range, spanning the Zn3P2 band gaps. Purity and integrity of the Zn3P2 microwires including infrared light scattering properties were confirmed by infrared transmission microscopy. This biomimetic microwire shows promise for infrared chip development. 10. Design of uniphase inverter based on LM3S-811%基于LM3S-811单相逆变器设计 Institute of Scientific and Technical Information of China (English) 王文涛; 鲁金钿; 彭瑞 2014-01-01 The high frequency inverter based on LM3S-811 was realized with the design. The whole system is composed of auxiliary power supply,push-pull booster,full-bridge inverter,SPWM wave generator,over-current protection module,low pass filtering module,etc. 12 V DC is converted into a high frequency square wave by push-pull booster,and then is converted into 300 V DC by transformer boosting,rectification,filtering. SG3525 is used in the push-pull module to drive MOSFET for getting high voltage DC. LM3S-811 is used to produce SPWM wave for driving the full-bridge inversion module,and is com-bined with low pass filtering and output over-current protection to get 220 V AC power frequency. Its output power is up to 240 W. This system has the advantages of small volume and stable output.%在此实现了基于LM3S-811的高频逆变器，整个系统包括辅助电源、推挽升压、全桥逆变、SPWM产生、过流保护以及低通滤波等模块。DC 12 V低压直流经过挽推升压转换为高频方波，再经过变压器升压和整流、滤波转化为DC 300 V。推挽模块用SG3525驱动MOSFET得到高压直流电，再经过LM3S-811产生SPWM波驱动全桥逆模块并结合低通滤波、输出过流保护得到工频AC 220 V，输出功率可达240 W。该系统具有体积小、输出稳定等优点。 11. Study of Two-Photon Transistions in CLEO III Upsilon(3S) Data CERN Document Server Cinabro, D; McGee, S; Bornheim, A; Lipeles, E; Pappas, S P; Shapiro, A; Sun, W M; Weinstein, A J; Mahapatra, R; Briere, R A; Chen, G P; Ferguson, T; Tatishvili, G T; Vogel, H; Adam, N E; Alexander, J P; Berkelman, K; Boisvert, V; Cassel, David G; Drell, P S; Duboscq, J E; Ecklund, K M; Ehrlich, R; Galik, R S; Gibbons, L; Gittelman, B; Gray, S W; Hartill, D L; Heltsley, B K; Hsu, L; Jones, C D; Kandaswamy, J; Kreinick, D L; Magerkurth, A; Mahlke-Krüger, H; Meyer, T O; Mistry, N B; Nordberg, E; Patterson, J R; Peterson, D; Pivarski, J; Riley, D; Sadoff, A J; Schwarthoff, H; Shepherd, M R; Thayer, J G; Urner, D; Viehhauser, G; Warburton, A; Weinberger, M; Athar, S B; Avery, P; Breva-Newell, L; Potlia, V; Stöck, H; Yelton, J; Brandenburg, G; Kim, D Y J; Wilson, R; Benslama, K; Eisenstein, B I; Ernst, J; Gollin, G D; Hans, R M; Karliner, I; Lowrey, N; Plager, C; Sedlack, C; Selen, M; Thaler, J J; Williams, J; Edwards, K W; Ammar, R; Besson, D; Zhao, X; Anderson, S; Frolov, V V; Kubota, Y; Lee, S J; Li, S Z; Poling, R A; Smith, A; Stepaniak, C J; Urheim, J; Metreveli, Z V; Seth, K K; Tomaradze, A G; Zweber, P; Ahmed, S; Alam, M S; Jian, L; Saleem, M; Wappler, F; Eckhart, E; Gan, K K; Gwon, C; Hart, T; Honscheid, K; Hufnagel, D; Kagan, H; Kass, R; Pedlar, T K; Thayer, J B; Von Törne, E; Wilksen, T; Zoeller, M M; Muramatsu, H; Richichi, S J; Severini, H; Skubic, P L; Dytman, S A; Müller, J A; Nam, S; Savinov, V; Chen, S; Hinson, J W; Lee, J; Miller, D H; Pavlunin, V; Shibata, E I; Shipsey, I P J; Cronin-Hennessy, D; Lyon, A L; Park, C S; Park, W; Thorndike, E H; Coan, T E; Gao, Y S; Liu, F; Maravin, Y; Stroynowski, R; Artuso, M; Boulahouache, C; Bukin, K; Dambasuren, E; Khroustalev, K; Mountain, R; Nandakumar, R; Skwarnicki, T; Stone, S; Wang, J C; Mahmood, A H; Csorna, S E; Danko, I 2002-01-01 We have studied two-photon transitions from Upsilon(3S) decays recorded by the CLEO-III detector in exclusive events with two photons and either two electrons or two muons. We obtain precision measurements of the chi_b(2P_J) masses for J=2 and J=1. The transition rates for all three spin states of the 2P triplet are measured with improved precision which leads to a better determination of their hadronic width ratios. We also observe rare transitions via the chi_b(1P) states. The measured rates for these transitions allow a determination of , the E1 matrix element, which is more sensitive to the structure of the bb states than the matrix element which dominates the radiative decays of the Upsilon(3S) state. We also present first upper limits on the branching ratios for Upsilon(3S)-> pi0 Upsilon(2S) Upsilon(3S) -> pi0 Upsilon(1S) and a new limit for the branching ratio for Upsilon(3S) -> eta Upsilon(1S). 12. Group 3 innate lymphoid cells (ILC3s): Origin, differentiation, and plasticity in humans and mice. Science.gov (United States) Montaldo, Elisa; Juelke, Kerstin; Romagnani, Chiara 2015-08-01 Since their discovery, innate lymphoid cells (ILCs) have been the subject of intense research. As their name implies, ILCs are innate cells of lymphoid origin, and can be grouped into subsets based on their cytotoxic activity, cytokine profile, and the transcriptional requirements during ILC differentiation. The main ILC groups are "killer" ILCs, comprising NK cells, and "helper-like" ILCs (including ILC1s, ILC2s, and ILC3s). This review examines the origin, differentiation stages, and plasticity of murine and human ILC3s. ILC3s express the retinoic acid receptor (RAR) related orphan receptor RORγt and the signature cytokines IL-22 and IL-17. Fetal ILC3s or lymphoid tissue inducer cells are required for lymphoid organogenesis, while postnatally developing ILC3s are important for the generation of intestinal cryptopatches and isolated lymphoid follicles as well as for the defence against pathogens and epithelial homeostasis. Here, we discuss the transcription factors and exogenous signals (including cytokines, nutrients and cell-to-cell interaction) that drive ILC3 lineage commitment and acquisition of their distinctive effector program. 13. PTTG1 regulated by miR-146a-3p promotes bladder cancer migration, invasion, metastasis and growth Science.gov (United States) Xiang, Wei; Wu, Xinchao; Huang, Chao; Wang, Miao; Zhao, Xian; Luo, Gang; Li, Yawei; Jiang, Guosong; Xiao, Xingyuan; Zeng, Fuqing 2017-01-01 Pituitary tumor-transforming gene 1 (PTTG1) is identified as an oncogene, and overexpresses in many tumors. However, the role of PTTG1 in bladder cancer (BC) hasn't yet been characterized well. In this study, we showed the expression of PTTG1 mRNA and protein were both significantly increased in BC tissues and cells. The PTTG1 protein levels were positive correlated with increased tumor size, tumor–node–metastasis (TNM) stage, lymphatic invasion and distant metastasis of BC. PTTG1 knockdown dramatically suppressed the migration, invasion, metastasis and growth, and induced senescence and cell-cycle arrest at G0/G1 phase of BC cells. We further identified PTTG1 was the direct target of miR-146a-3p through using target prediction algorithms and luciferase reporter assay. miR-146a-3p was low expressed and negatively correlated with PTTG1 levels in BC tissues and cells. miR-146a-3p overexpression inhibited migration, invasion, metastasis and growth, and induced senescence of BC cells. Rescue experiment suggested ectopic expression of miR-146a-3p and PTTG1 suppressed migration, invasion and induced cell cycle arrest and senescence of BC cells compared to PTTG1 overexpression, confirming miR-146a-3p inhibited BC progression by targeting PTTG1. In summary, our study found miR-146a-3p/PTTG1 axis regulated BC migration, invasion, metastasis and growth, and might be a targets for BC therapy. PMID:27893422 14. [Effect of miR-342-3p on chemotherapy sensitivity in triple-negative breast cancer]. Science.gov (United States) Ma, Tao; Zhang, Junying; Wu, Jianzhong; Tang, Jinhai 2014-05-01 To study the effect of miR-342-3p on the chemotherapy sensitivity in triple-negative breast cancer (TNBC). Tissue samples were collected from January 2011 to August 2013 samples in Jiangsu Cancer Hospital from a total of 32 triple-negative breast cancer patients with preoperative chemotherapy, with 5 cases of complete response (CR) and 27 cases of partial response (PR). We detected miR-342-3p expression of TNBC with RT-PCR. We transfected has-miR-342-3p mimic and inhibitor into breast cancer cell lines MDA-MB-231 by lipofection transfection and set up negative control mim-NC and inhi-NC. Group of mimic, mim-NC, inhibitor and inhi-NC were cultivated with 2 μmol /L paclitaxel, cisplatin or 4 μmol/L doxorubicin for 48 h. The cell growth rates were measured by CCK8 reagent kit, and the cell apoptosis rate by flow cytometry. The expressions of miRNA-342-3p in TNBC tissue of CR were higher than those of PR. The cell growth rates of mimic were lower and cell apoptosis rates were higher than those of min- NC after cultivating with paclitaxel or cisplatin for 48 h, with significant difference (P0.05). TNBC with high expression of miR-342-3p are more sensitive to chemotherapy. miRNA-342-3p may regulate the sensitivity of breast cancer cell line MDA-MB-231 to chemotherapy drugs paclitaxel and cisplatin, but can not affect the chemotherapy sensitivity of doxorubicin. 15. Energetics, transition states, and intrinsic reaction coordinates for reactions associated with O(3P) processing of hydrocarbon materials Science.gov (United States) Yan, Tianying; Hase, William L.; Doubleday, Charles 2004-05-01 Electronic structure calculations based on multiconfiguration wave functions are used to investigate a set of archetypal reactions relevant to O(3P) processing of hydrocarbon molecules and surfaces. These include O(3P) reactions with methane and ethane to give OH plus methyl or ethyl radicals, O(3P)+ethane to give CH3O+CH3, and secondary reactions of the OH product radical with ethane and the ethyl radical. Geometry optimization is carried out with CASSCF/cc-pVTZ for all reactions, and with CASPT2/cc-pVTZ for O(3P)+methane/ethane. Single-point energy corrections are applied with CASPT2, CASPT3, and MRCI+Q with the cc-pVTZ and cc-pVQZ basis sets, and the energies extrapolated to the complete basis set limit (CBL). Where comparison of computed barriers and energies of reaction with experiment is possible, the agreement is good to excellent. The best agreement (within experimental error) is found for MRCI+Q/CBL applied to O(3P)+methane. For the other reactions, CASPT2/CBL and MRCI+Q/CBL predictions differ from experiment by 1-5 kcal/mol for 0 K enthalpies of reaction, and are within 1 kcal/mol of the best-estimate experimental range of 0 K barriers for O(3P)+ethane and OH+ethane. The accuracy of MRCI+Q/CBL is limited mainly by the quality of the active space. CASPT2/CBL barriers are consistently lower than MRCI+Q/CBL barriers with identical reference spaces. 16. Na/K-ATPase signaling regulates collagen synthesis through microRNA-29b-3p in cardiac fibroblasts. Science.gov (United States) Drummond, Christopher A; Hill, Michael C; Shi, Huilin; Fan, Xiaoming; Xie, Jeffrey X; Haller, Steven T; Kennedy, David J; Liu, Jiang; Garrett, Michael R; Xie, Zijian; Cooper, Christopher J; Shapiro, Joseph I; Tian, Jiang 2016-03-01 Chronic kidney disease (CKD) is accompanied by cardiac fibrosis, hypertrophy, and dysfunction, which are commonly referred to as uremic cardiomyopathy. Our previous studies found that Na/K-ATPase ligands or 5/6th partial nephrectomy (PNx) induces cardiac fibrosis in rats and mice. The current study used in vitro and in vivo models to explore novel roles for microRNA in this mechanism of cardiac fibrosis formation. To accomplish this, we performed microRNA profiling with RT-qPCR based arrays on cardiac tissue from rats subjected to marinobufagenin (MBG) infusion or PNx. The analysis showed that a series of fibrosis-related microRNAs were dysregulated. Among the dysregulated microRNAs, microRNA (miR)-29b-3p, which directly targets mRNA of collagen, was consistently reduced in both PNx and MBG-infused animals. In vitro experiments demonstrated that treatment of primary cultures of adult rat cardiac fibroblasts with Na/K-ATPase ligands induced significant increases in the fibrosis marker, collagen protein, and mRNA expression compared with controls, whereas miR-29b-3p expression decreased >50%. Transfection of miR-29b-3p mimics into cardiac fibroblasts inhibited cardiotonic steroids-induced collagen synthesis. Moreover, a specific Na/K-ATPase signaling antagonist, pNaKtide, prevented ouabain-induced increases in collagen synthesis and decreases in miR-29b-3p expression in these cells. In conclusion, these data are the first to indicate that signaling through Na/K-ATPase regulates miRNAs and specifically, miR-29b-3p expression both in vivo and in vitro. Additionally, these data indicate that miR-29b-3p expression plays an important role in the formation of cardiac fibrosis in CKD. 17. Ferrocene-Functionalized Cu(I)/Ag(I) Dithiocarbamate Clusters. Science.gov (United States) Kishore, Pilli V V N; Liao, Jian-Hong; Hou, Hsing-Nan; Lin, Yan-Ru; Liu, C W 2016-04-04 A series of compounds, namely, [Cu8(μ4-H){S2CNMeCH2Fc}6](PF6) (1), [Cu7(μ4-H) {S2CN(i)PrCH2Fc}6] (2), [Cu3{S2CN(Bz) (CH2Fc)}2(dppf)2](PF6) (3), and [Ag2{S2CNMe(CH2Fc)}2(PPh3)2] (4) (dppf = 1,1'-bis(diphenylphosphino)ferrocene), supported by multiferrocene assemblies, were synthesized. All the compounds were characterized by (1)H NMR, Fourier transform infrared, elemental analysis, and electrospray ionization mass spectrometry techniques. Single-crystal X-ray structural analysis revealed that 1 is a monocationic octanuclear Cu(I) cluster and that 2 is a neutral heptanuclear Cu(I) cluster with tetracapped tetrahedral (1) and tricapped tetrahedral (2) geometries entrapped with an interstitial hydride, anchored by six ferrocene units at the periphery of the core. Compounds 3 and 4 comprise trimetallic Cu(I) and dimetallic Ag(I) cores enfolded by four and two ferrocene moieties. Interestingly both chelating and bridging modes of binding are observed for dppf ligand in 3. Further the formation and isolation of polyhydrido copper clusters [Cu28H15{S2CN(i)PrCH2Fc}12](PF6) (5) and [Cu28H15{S2CN(n)Bu2}12](PF6) (7), stabilized by bulky ferrocenyl and n-butyl dithiocarbamate ligands, was demonstrated. They are readily identified by (2)H NMR studies on their deuterium analogues, [Cu28D15{S2CN(i)PrCH2Fc}12](PF6) (6) and [Cu28D15{S2CN(n)Bu2}12](PF6) (8). Though the structure details as well as spectroscopic characterizations of 5 are yet to be investigated, the compound 7 is fully characterized by variety of spectroscopy including single-crystal X-ray diffraction. The cyclic voltammetry studies for compounds 1, 2, and 4 display irreversible redox peaks for Fe(2+)/Fe(3+) couple wherein the reduction peaks are not well-resolved due to some adsorption of the complex onto the electrode surface. 18. Delineation of the 3p14.1p13 microdeletion associated with syndromic distal limb contractures DEFF Research Database (Denmark) Thevenon, Julien; Monnier, Nicole; Callier, Patrick 2014-01-01 Distal limb contractures (DLC) represent a heterogeneous clinical and genetic condition. Overall, 20-25% of the DLC are caused by mutations in genes encoding the muscle contractile apparatus. Large interstitial deletions of the 3p have already been diagnosed by standard chromosomal analysis......, but not associated with a specific phenotype. We report on four patients with syndromic DLC presenting with a de novo 3p14.1p13 microdeletion. The clinical features associated multiple contractures, feeding problems, developmental delay, and intellectual disability. Facial dysmorphism was constant with low... 19. A proof of the linearity conjecture for k-blocking sets in PG(n, p3), p prime CERN Document Server Lavrauw, Michel; Van de Voorde, Geertrui 2012-01-01 In this paper, we show that a small minimal k-blocking set in PG(n, q3), q = p^h, h >= 1, p prime, p >=7, intersecting every (n-k)-space in 1 (mod q) points, is linear. As a corollary, this result shows that all small minimal k-blocking sets in PG(n, p^3), p prime, p >=7, are Fp-linear, proving the linearity conjecture (see [7]) in the case PG(n, p3), p prime, p >= 7. 20. SOX9 regulates microRNA miR-202-5p/3p expression during mouse testis differentiation DEFF Research Database (Denmark) Wainwright, Elanor N; Jorgensen, Joan S; Kim, Youngha; 2013-01-01 . Expression of the primary transcript of miR-202-5p/3p remained low in XY gonads in a conditional Sox9-null mouse model, suggesting that pri-miR-202 transcription is downstream of SOX9, a transcription factor that is both necessary and sufficient for male sex determination. We identified the pri-miR-202...... findings indicate that expression of the conserved gonad microRNA, miR-202-5p/3p, is downstream of the testis-determining factor SOX9, suggesting an early role in testis development.... 1. Lifetime measurement of the metastable 2{sup 3}P{sub 0} state in He-like uranium Energy Technology Data Exchange (ETDEWEB) Toleikis, S. [Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., MS 88R0192, Berkeley, CA 94720-8101 (United States) and Texas A and M University, College Station, TX (United States)]. E-mail: stoleikis@lbl.gov; Manil, B. [GANIL, Caen (France); Bednarz, G. [IFUJ, Cracow (Poland); Berdermann, E. [GSI, Darmstadt (Germany); Beyer, H.F. [GSI, Darmstadt (Germany); Bosch, F. [GSI, Darmstadt (Germany); Braeuning-Demian, A. [GSI, Darmstadt (Germany); Gumberidze, A. [GSI, Darmstadt (Germany); Indelicato, P. [Universite P. et M. Curie, Paris (France); Kozhuharov, C. [GSI, Darmstadt (Germany); Liesen, D. [GSI, Darmstadt (Germany); Marrus, R. [University of California, Berkeley, CA (United States); Mokler, P.H. [GSI, Darmstadt (Germany); Stachura, Z. [INP, Cracow (Poland); Stoehlker, T. [GSI, Darmstadt (Germany); Warczak, A. [IFUJ, Cracow (Poland) 2005-07-01 The lifetime of the 2{sup 3}P{sub 0} state in He-like uranium has been measured in a beam-foil time-of-flight experiment at the Gesellschaft fur Schwerionenforschung accelerator facility with the result {tau}(2{sup 3}P{sub 0})=58.2(9.5)ps. With the measured lifetime it is possible to derive a value of {delta}E{sub 2s}{sup Lamb}=76.3+/-20.6eV for the n=2 Lamb shift in uranium. 2. miR-199a-3p 靶基因预测及生物信息学分析%Prediction of miR-199a-3p targets gene and its bioinformatics analysis Institute of Scientific and Technical Information of China (English) 谢小娟; 潘晶晶; 魏力强; 陈葳 2016-01-01 Objective To provide theoretical guidance for further research on the role of miR-1 99a-3p in formation and development of bladder cancer.Methods Mature sequence of miR-1 99a-3p was analyzed;target genes and transcription factors of miRNA-1 99a-3p were predicted,and the target genes were analyzed for gene ontology (GO)enrichment and Kyoto Encyclopedia of Genes and Genome (KEGG)pathway.Then TF-miRNA-mRNA network diagram was constructed.Results Sequences of miR-1 99a-3p were highly conserved in various species.In GO analysis,the target genes of miR-1 99a-3p were enriched in many biological processes,such as regulation of cellular process,regulation of macromolecule metabolic process,and regulation of biological process (P <0.01 ).In KEGG pathway,the target genes were mainly located in bacterial invasion pathway of epithelial cells,ECM-receptor interaction pathway,PI3K-Akt signaling pathway,MAPK signaling pathway,small cell lung cancer pathway,and proteoglycans pathway in the cancer (P <0.05).According to the TF-miRNA-mRNA network diagram,the important genes that might be regulated by miR-1 99a-3p were MYC,SP1,mTOR,NFκB,and NFκB1.Conclusion miR-1 99a-3p may directly target mTOR and participate in the formation and development of bladder cancer through regulating PI3K-Akt-mTOR signaling pathway.%目的：为深入研究 miR-199a-3p 在膀胱癌形成和发展中的作用提供理论依据。方法分析 miR-199a-3p 序列,预测其靶基因和转录因子,并对靶基因进行 GO 富集和 KEGG Pathway 分析；构建 TF-miR-199a-3p-靶基因网络调控图。结果miR-199a-3p 序列在多物种间具有高度保守性；GO 分析发现 miR-199a-3p 的靶基因参与细胞调节、代谢调节、细胞大分子生物合成等生物过程(P <0.01)；KEGG Pathway 分析发现 miR-199a-3p 的靶基因显著富集在上皮细胞的细菌入侵通路、ECM 受体的相互作用通路、PI3K-Akt 信号通路、MAPK 信号通路、小细胞肺癌通路、癌症中的蛋白 3. High lying energy positions of doubly (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} excited states of the beryllium atom Energy Technology Data Exchange (ETDEWEB) Sakho, I., E-mail: aminafatima_sakho@yahoo.fr [UFR Sciences and Technologies, Department of physics, University of Ziguinchor, Ziguinchor (Senegal) 2011-12-15 The Screening Constant by Unit Nuclear Charge (SCUNC) method is used to study (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} autoionizing states of the beryllium atom. Energy positions are reported up to n=20. In addition, resonance widths of the (2pns) {sup 1}P{sup o} states also presented. The current results compared very well to available theoretical and experimental literature values up to n=15. The accurate data presented in this work may be of interest for future experimental and theoretical studies in the photoabsorption spectrum of Be. - Highlights: > Accurate energy positions of (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} (n=3-20) autoionizing states of Be atoms. > Currently results compared very well to theoretical and experimental literature values up to n=15. > Presently data may be of interest for future experimental and theoretical studies in the photoabsorption spectrum of Be. 4. Microstructure and Wear Behaviors of In-situ Al2O3p/7075 Composites%原位Al2O3P/7075复合材料微观组织与磨损行为 Institute of Scientific and Technical Information of China (English) 刘慧敏; 杨树青; 许萍; 李进福 2012-01-01 采用原位反应近液相线铸造法制备具有不同质量分数的Al2O3P/7075复合材料,并对其进行干滑动磨损实验研究,通过OM,SEM,TEM等材料分析方法测试了材料的微观组织和磨损表面形貌.结果表明,原位Al2O3颗粒对7075铝合金的晶粒组织有明显细化效果,Al2O3P/7075复合材料的耐磨性比基体7075铝合金有明显的提高.原因是原位合成的复合材料界面结合良好,原位Al2O3颗粒在摩擦过程中起着抑制金属流动和支撑的双重作用.磨损表面形貌显示,原位Al2O3颗粒的加入,使磨损机制由黏着磨损变为磨粒磨损,从而改善了材料的耐磨性.%The Al2O3P/7075 Al composites were synthesized by in-situ reaction near-liquidus casting. The microstructure and dry sliding wear behavior of the prepared composites were analyzed using OM, SEM and TEM as well as wear friction testing. The results reveal that in-situ Al2O3 particle with average size of approximately <1μm is uniformly distributed in the matrix, which exhibits desirable refining effects on microstructure of 7075 Al alloy. The wear behavior of the composites is greatly superior to that of the matrix, which is attributed to the grain refining and formation of a compact interface of Al2O3P/7075 Al composites. In addition, in-situ Al2O3 particles exhibit coupling effects of inhibiting metal flow and bearing some load in process of friction. The wear mechanism of the matrix 7075A1 alloy is adhesive wear, while the wear surface of Al2O3p/7075 Al composites is superior to that of the matrix 7075 Al alloy. The wear mechanism of Al2O3P/7075 Al composites is abrasive wear, and wear resistance of Al2O3P/7075 Al is improved. 5. Heazlewoodite, Ni3S2: A Potent Catalyst for Oxygen Reduction to Water under Benign Conditions. Science.gov (United States) Falkowski, Joseph M; Concannon, Nolan M; Yan, Bing; Surendranath, Yogesh 2015-07-01 Electrodeposited thin films and nanoparticles of Ni3S2 are highly active, poison- and corrosion-resistant catalysts for oxygen reduction to water at neutral pH. In pH 7 phosphate buffer, Ni3S2 displays catalytic onset at 0.8 V versus the reversible hydrogen electrode, a Tafel slope of 109 mV decade(-1), and high faradaic efficiency for four-electron reduction of O2 to water. Under these conditions, the activity and stability of Ni3S2 exceeds that of polycrystalline platinum and manganese, nickel, and cobalt oxides, illustrating the catalytic potential of pairing labile first-row transition metal active sites with a more covalent sulfide host lattice. 6. Phospholipid flippases Lem3p-Dnf1p and Lem3p-Dnf2p are involved in the sorting of the tryptophan permease Tat2p in yeast. Science.gov (United States) Hachiro, Takeru; Yamamoto, Takaharu; Nakano, Kenji; Tanaka, Kazuma 2013-02-01 The type 4 P-type ATPases are flippases that generate phospholipid asymmetry in membranes. In budding yeast, heteromeric flippases, including Lem3p-Dnf1p and Lem3p-Dnf2p, translocate phospholipids to the cytoplasmic leaflet of membranes. Here, we report that Lem3p-Dnf1/2p are involved in transport of the tryptophan permease Tat2p to the plasma membrane. The lem3Δ mutant exhibited a tryptophan requirement due to the mislocalization of Tat2p to intracellular membranes. Tat2p was relocalized to the plasma membrane when trans-Golgi network (TGN)-to-endosome transport was inhibited. Inhibition of ubiquitination by mutations in ubiquitination machinery also rerouted Tat2p to the plasma membrane. Lem3p-Dnf1/2p are localized to endosomal/TGN membranes in addition to the plasma membrane. Endocytosis mutants, in which Lem3p-Dnf1/2p are sequestered to the plasma membrane, also exhibited the ubiquitination-dependent missorting of Tat2p. These results suggest that Tat2p is ubiquitinated at the TGN and missorted to the vacuolar pathway in the lem3Δ mutant. The NH(2)-terminal cytoplasmic region of Tat2p containing ubiquitination acceptor lysines interacted with liposomes containing acidic phospholipids, including phosphatidylserine. This interaction was abrogated by alanine substitution mutations in the basic amino acids downstream of the ubiquitination sites. Interestingly, a mutant Tat2p containing these substitutions was missorted in a ubiquitination-dependent manner. We propose the following model based on these results; Tat2p is not ubiquitinated when the NH(2)-terminal region is bound to membrane phospholipids, but if it dissociates from the membrane due to a low level of phosphatidylserine caused by perturbation of phospholipid asymmetry in the lem3Δ mutant, Tat2p is ubiquitinated and then transported from the TGN to the vacuole. 7. MicroRNA-19b-3p Modulates Japanese Encephalitis Virus-Mediated Inflammation via Targeting RNF11. Science.gov (United States) Ashraf, Usama; Zhu, Bibo; Ye, Jing; Wan, Shengfeng; Nie, Yanru; Chen, Zheng; Cui, Min; Wang, Chong; Duan, Xiaodong; Zhang, Hao; Chen, Huanchun; Cao, Shengbo 2016-05-01 Japanese encephalitis virus (JEV) can invade the central nervous system and consequently induce neuroinflammation, which is characterized by profound neuronal cell damage accompanied by astrogliosis and microgliosis. Albeit microRNAs (miRNAs) have emerged as major regulatory noncoding RNAs with profound effects on inflammatory response, it is unknown how astrocytic miRNAs regulate JEV-induced inflammation. Here, we found the involvement of miR-19b-3p in regulating the JEV-induced inflammatory responsein vitroandin vivo The data demonstrated that miR-19b-3p is upregulated in cultured cells and mouse brain tissues during JEV infection. Overexpression of miR-19b-3p led to increased production of inflammatory cytokines, including tumor necrosis factor alpha, interleukin-6, interleukin-1β, and chemokine (C-C motif) ligand 5, after JEV infection, whereas knockdown of miR-19b-3p had completely opposite effects. Mechanistically, miR-19b-3p modulated the JEV-induced inflammatory response via targeting ring finger protein 11, a negative regulator of nuclear factor kappa B signaling. We also found that inhibition of ring finger protein 11 by miR-19b-3p resulted in accumulation of nuclear factor kappa B in the nucleus, which in turn led to higher production of inflammatory cytokines.In vivosilencing of miR-19b-3p by a specific antagomir reinvigorates the expression level of RNF11, which in turn reduces the production of inflammatory cytokines, abrogates gliosis and neuronal cell death, and eventually improves the survival rate in the mouse model. Collectively, our results demonstrate that miR-19b-3p positively regulates the JEV-induced inflammatory response. Thus, miR-19b-3p targeting may constitute a thought-provoking approach to rein in JEV-induced inflammation. Japanese encephalitis virus (JEV) is one of the major causes of acute encephalitis in humans worldwide. The pathological features of JEV-induced encephalitis are inflammatory reactions and neurological diseases 8. Crystal Structure of 3S-hydroxy-7 Melleine%3S-7-羟基蜂蜜曲霉素分子的晶体结构 Institute of Scientific and Technical Information of China (English) 刘晓红; 许芳; 张奕; 刘丽红; 黄华容; 佘志刚; 林永成; 陈荣礼 2006-01-01 从红树林内生真菌Xylariasp.2508中分离到一个新的蜂蜜曲霉素型化合物3S-7-羟基蜂蜜曲霉素,这是首次从海洋真菌中获得该类结构的化合物并测定其晶体结构.通过核磁共振谱、红外光谱、质谱进行结构鉴定.其单晶经X射线衍射测试表明,该化合物晶体属单斜晶系,空间群为P21,化学式为C10H12O5,Mr=212.20.晶胞参数为:a=10.8884(19)(A),b=7.2284(13)(A),c=13.398(2)(A),β=104.217(3)°,V=1022.2(3)(A)3,Z=4,Dc=1.379 mg/m3,F(000)=448,μ=0.112 mm-1.结构由直接法解出,用全矩阵最小二乘法修正,最终偏离因子R=0.0498,wR=0.101.该分子由一个苯并吡喃环组成.通过与文献中已知3R-7-羟基蜂蜜曲霉素的熔点及旋光度对照,确定其绝对构型为3S.其初步抑菌实验结果表明,3S-7-羟基蜂蜜曲霉素菌在每纸片200 μg的剂量下对金黄色葡萄球菌(Staphylococcus aureus)没有表现出抑菌活性.%A new compound, 3S-hydroxy-7 melleine was isolated from the endophytic fungus Xylariasp No.2508 from the mangrove tree on the South China Sea coast. It was the first time that this kind of compound was isolated from marine fungus. The structure was elucidated by NMR data, infrared spectrum (IR) and mass spectrometry (MS). In addition, its structure was determined by the single-crystal X-ray diffraction analysis. It crystallized in monoclinic, space group P21 with a=10.8884(19) (A), b=7.2284(13) (A), c=13.398(2) (A), β=104.217(3)°,C10H10O4·H2O, Mr=212.20, V=1022.2(3) (A)3, Z=4, Dc=1.379 mg/m3, F(000)=448, μ=0.112 mm-1, the final R=0.0498, wR=0.101 for 2407 observed reflections (I＞2σ(I)). The molecular backbone of the compound includes a benzopyran ring. By comparing with the melting point and the optical rotation of the known 3R-hydroxy-7 melleine in literature, the absolute configuration of the compound was determined as 3S. It didn't exhibit antibacterial activity against Gram-positive bacterium Staphylococcus aureus at 200μg/disk in the 9. Structure-dependent size effects in CuTa/Cu nanolaminates Energy Technology Data Exchange (ETDEWEB) Gu, C. [State-Key Laboratory for Mechanical Behavior of Material, Xi’an Jiaotong University Xi’an, 710049 (China); Wang, F., E-mail: wangfei@mail.xjtu.edu.cn [State Key Laboratory for Strength and Vibration of Mechanical Structures, Xian Jiaotong University Xi’an, 710049 (China); Huang, P., E-mail: huangping@mail.xjtu.edu.cn [State-Key Laboratory for Mechanical Behavior of Material, Xi’an Jiaotong University Xi’an, 710049 (China); Xu, K.W. [State-Key Laboratory for Mechanical Behavior of Material, Xi’an Jiaotong University Xi’an, 710049 (China); Lu, T.J. [State Key Laboratory for Strength and Vibration of Mechanical Structures, Xian Jiaotong University Xi’an, 710049 (China) 2016-03-21 CuTa monolayers with different Ta contents (34 at% and 37 at%) and CuTa/Cu multilayers with varying nanoscale Cu layer thickness (3–24 nm) were prepared by magnetron sputtering. Their microstructure and mechanical properties were investigated by means of high resolution transmission electron microscopy (HRTEM) and nanoindentation testing. The microstructure of CuTa34 was pure amorphous phase while that of CuTa37 was amorphous phase embedded with nanoparticles. Adding Cu layers into the two different CuTa monolayers led to CuTa-Cu interfaces with different microstructures. For CuTa34/Cu, the interface was relatively straight and the Cu layers exhibited textured growth. For CuTa37/Cu, the interface was wavy and GBs (grain boundaries) were formed in the Cu layers. To investigate the influence of the two different microstructures on multilayer deformation, the residue indentation morphologies of CuTa/Cu were observed under scanning electronic microscope (SEM). For both CuTa34/Cu and CuTa37/Cu, SBs (shear bands) could be effectively inhibited if the Cu layers had a proper thickness. Dominant deformation mechanisms and size effects were proposed and discussed for CuTa/Cu thin films possessing different interfaces and Cu layer structures. 10. The effect of urea on microstructures of Ni3S2 on nickel foam and its hydrogen evolution reaction Science.gov (United States) Jinlong, Lv; Tongxiang, Liang 2016-11-01 The effects of urea concentration on microstructures of Ni3S2formed on nickel foam and its hydrogen evolution reaction were investigated. The Ni3S2 nanosheets with porous structure were formed on nickel foam during hydrothermal process due to low urea concentration. While high urea concentration facilitated the forming of Ni3S2 nanotube arrays. The resulting Ni3S2 nanotube arrays exhibited higher catalytic activity than Ni3S2nanosheets for hydrogen evolution reaction. This was mainly attributed to a fact that Ni3S2 nanotube arrays facilitated diffusion of electrolyte for hydrogen evolution reaction. 11. Search for eta_b(1S) in Inclusive Radiative Decays of the Upsilon(3S) CERN Document Server Mahmood, A H; Danko, I; Bonvicini, G; Cinabro, D; Dubrovin, M; McGee, S; Bornheim, A; Lipeles, E; Pappas, S P; Shapiro, A; Sun, W M; Weinstein, A J; Mahapatra, R; Briere, R A; Chen, G P; Ferguson, T; Tatishvili, G T; Vogel, H; Adam, N E; Alexander, J P; Berkelman, K; Boisvert, V; Cassel, David G; Drell, P S; Duboscq, J E; Ecklund, K M; Ehrlich, R; Galik, R S; Gibbons, L; Gittelman, B; Gray, S W; Hartill, D L; Heltsley, B K; Hsu, L; Jones, C D; Kandaswamy, J; Kreinick, D L; Magerkurth, A; Mahlke-Krüger, H; Meyer, T O; Mistry, N B; Nordberg, E; Patterson, J R; Peterson, D; Pivarski, J; Riley, D; Sadoff, A J; Schwarthoff, H; Shepherd, M R; Thayer, J G; Urner, D; Viehhauser, G; Warburton, A; Weinberger, M; Athar, S B; Avery, P; Breva-Newell, L; Potlia, V; Stöck, H; Yelton, J; Brandenburg, G; Kim, D Y J; Wilson, R; Benslama, K; Eisenstein, B I; Ernst, J; Gollin, G D; Hans, R M; Karliner, I; Lowrey, N; Plager, C; Sedlack, C; Selen, M; Thaler, J J; Williams, J; Edwards, K W; Ammar, R; Besson, D; Zhao, X; Anderson, S; Frolov, V V; Kubota, Y; Lee, S J; Li, S Z; Poling, R A; Smith, A; Stepaniak, C J; Urheim, J; Metreveli, Z V; Seth, K K; Tomaradze, A G; Zweber, P; Ahmed, S; Alam, M S; Jian, L; Saleem, M; Wappler, F; Eckhart, E; Gan, K K; Gwon, C; Hart, T; Honscheid, K; Hufnagel, D; Kagan, H; Kass, R; Pedlar, T K; Thayer, J B; Von Törne, E; Wilksen, T; Zoeller, M M; Muramatsu, H; Richichi, S J; Severini, H; Skubic, P L; Dytman, S A; Müller, J A; Nam, S; Savinov, V; Chen, S; Hinson, J W; Lee, J; Miller, D H; Pavlunin, V; Shibata, E I; Shipsey, I P J; Cronin-Hennessy, D; Lyon, A L; Park, C S; Park, W; Thorndike, E H; Coan, T E; Gao, Y S; Liu, F; Maravin, Y; Stroynowski, R; Artuso, M; Boulahouache, C; Bukin, K; Dambasuren, E; Khroustalev, K; Mountain, R; Nandakumar, R; Skwarnicki, T; Stone, S; Wang, J C 2002-01-01 We have searched for the bottomonium eta_b(1S) via the hindered magnetic dipole (M1) photon transition Upsilon(3S) -> gamma eta_b(1S). No evidence for such a transition is found in the data sample of 4.7 x 10^6 Upsilon(3S)'s collected with the CLEO-III detector. We set upper limits on the branching ratio from 9.30 to 9.43 GeV/c^2 of eta_b(1S) masses. These upper limits rule out many previously published phenomenological estimates of the rate for this transition. 12. 4th International Symposium on Sensor Science (I3S2015: Conference Report Directory of Open Access Journals (Sweden) Peter Seitz 2015-09-01 Full Text Available An international scientific conference was sponsored by the journal Sensors under the patronage of the University of Basel. The 4th edition of the International Symposium on Sensor Science (I3S2015 ran from 13 to 15 July 2015 in Basel, Switzerland. It comprised five plenary sessions and one morning with three parallel sessions. The conference covered the most exciting aspects and the latest developments in sensor science. The conference dinner took place on the second evening of the conference. The I3S2015 brought together 170 participants from 40 different countries. [... 13. Theoretical Studies of the Reaction Mechanisms of CH3S + NO2 Institute of Scientific and Technical Information of China (English) 2002-01-01 The potential energy surface for the CH3S + NO2 reaction has been studied using the ab initio G3 (MP2) method. A variety of possible complexes and saddle points along the minimum energy reaction paths have been characterized at UMP2 (full)/6-31G(d) level. The calculations reveal dominating reaction mechanisms of the title reaction: CH3S + NO2 firstly produce intermediate CH3SONO, then break up into CH3SO + NO. The results are valuable to understand the atmospheric sulfur compounds oxidation mechanism. 14. Application of 3S Technology in Dynamic Monitoring of Capital Farmland Institute of Scientific and Technical Information of China (English) Xiaoyan; WANG; Guoye; REN 2013-01-01 Firstly,this paper introduces current situations of protection of capital farmland. According to current situations,it analyzes significance in protecting capital farmland and significance and functions of application of 3S technology in dynamic monitoring of capital farmland. With the aid of examples,it discusses functions of remote sensing and GIS in dynamic monitoring of capital farmland. It is believed that 3S technology not only can provide accurate parcel data of capital farmland changes for reviewing land change survey,but also can provide data for monitoring development situations of capital farmland,as well as provide basic current information for decision-making department. 15. Parity nonconservation effect with laser-induced 2^3S_1 - 2^1S_0 transition in heavy heliumlike ions CERN Document Server Shabaev, V M; Kozhuharov, C; Plunien, G; Stöhlker, Th 2010-01-01 The parity nonconservation (PNC) effect on the laser-induced 2^3S_1 - 2^1S_0 transition in heavy heliumlike ions is considered. A simple analytical formula for the PNC correction to the cross section is derived for the case, when the opposite-parity 2^1S_0 and 2^3P_0 states are almost degenerate and, therefore, the PNC effect is strongly enhanced. Numerical results are presented for heliumlike gadolinium and thorium, which seem most promising candidates for such kind of experiments. In both Gd and Th cases the photon energy required will be anticipated with a high-energy laser built at GSI. Alternatively, it can be gained with ultraviolet lasers utilizing relativistic Doppler tuning at FAIR facilities in Darmstadt. 16. Cyclo- and carbophosphazene-supported ligands for the assembly of heterometallic (Cu2+/Ca2+, Cu2+/Dy3+, Cu2+/Tb3+) complexes: synthesis, structure, and magnetism. Science.gov (United States) Chandrasekhar, Vadapalli; Senapati, Tapas; Dey, Atanu; Das, Sourav; Kalisz, Marguerite; Clérac, Rodolphe 2012-02-20 The carbophosphazene and cyclophosphazene hydrazides, [{NC(N(CH(3))(2))}(2){NP{N(CH(3))NH(2)}(2)}] (1) and [N(3)P(3)(O(2)C(12)H(8))(2){N(CH(3))NH(2)}(2)] were condensed with o-vanillin to afford the multisite coordination ligands [{NC(N(CH(3))(2))}(2){NP{N(CH(3))N═CH-C(6)H(3)-(o-OH)(m-OCH(3))}(2)}] (2) and [{N(2)P(2)(O(2)C(12)H(8))(2)}{NP{N(CH(3))N═CH-C (6)H(3)-(o-OH)(m-OCH(3))}(2)}] (3), respectively. These ligands were used for the preparation of heterometallic complexes [{NC(N(CH(3))(2))}(2){NP{N(CH(3))N═CH-C(6)H(3)-(o-O)(m-OCH(3))}(2)}{CuCa(NO(3))(2)}] (4), [{NC(N(CH(3))(2))}(2){NP{N(CH(3))N═CH-C(6)H(3)-(o-O)(m-OCH(3))}(2)}{Cu(2)Ca(2)(NO(3))(4)}]·4H(2)O (5), [{NC(N(CH(3))(2))}(2){NP{N(CH(3))N═CH-C(6)H(3)-(o-O)(m-OCH(3))}(2)}{CuDy(NO(3))(4)}]·CH(3)COCH(3) (6), [{NP(O(2)C(12)H(8))}(2){NP{N(CH(3))N═CH-C(6)H(3)-(o-O)(m-OCH(3))}(2)}{CuDy(NO(3))(3)}] (7), and [{NP(O(2)C(12)H(8))}(2){NP{N(CH(3))N═CH-C(6)H(3)-(o-O)(m-OCH(3))}(2)}{CuTb(NO(3))(3)}] (8). The molecular structures of these compounds reveals that the ligands 2 and 3 possess dual coordination pockets which are used to specifically bind the transition metal ion and the alkaline earth/lanthanide metal ion; the Cu(2+)/Ca(2+), Cu(2+)/Tb(3+), and Cu(2+)/Dy(3+) pairs in these compounds are brought together by phenoxide and methoxy oxygen atoms. While 4, 6, 7, and 8 are dinuclear complexes, 5 is a tetranuclear complex. Detailed magnetic properties on 6-8 reveal that these compounds show weak couplings between the magnetic centers and magnetic anisotropy. However, the ac susceptibility experiments did not reveal any out of phase signal suggesting that in these compounds slow relaxation of magnetization is absent above 1.8 K. 17. \\beta3p-spectroscopy and proton-\\gamma width determination in the decay of ^{31}Ar CERN Multimedia We propose to perform a detailed study of the \\beta-decay of the dripline nucleus ^{31}Ar. This will allow a detailed study of the \\beta-delayed 3p-decay as well as provide important information on the resonances of ^{30}S and ^{29}P, in particular the ratio between the p- and \\gamma- partial widths relevant for astrophysics. 18. Absence of mutations in the coding sequence of the potential tumor suppressor 3pK in metastatic melanoma Directory of Open Access Journals (Sweden) Houben Roland 2005-12-01 Full Text Available Abstract Background Activation of Ras or Raf contributes to tumorigenesis of melanoma. However, constitutive Raf activation is also a characteristic of the majority of benign melanocytic nevi and high intensity signaling of either Ras or Raf was found to induce growth inhibition and senescence rather than transformation. Since the chromosome 3p kinase (3pK is a target of the Ras/Raf/Mek/Erk signaling pathway which antagonizes the function of the oncogene and anti-differentiation factor Bmi-1, 3pK may function as a tumor suppressor in tumors with constitutive Ras/Raf activation. Consequently, we tested whether inactivating 3pK mutations are present in melanoma. Methods 30 metastatic melanoma samples, which were positive for activating mutations of either BRaf or NRas, were analyzed for possible mutations in the 3pk gene. The 10 coding exons and their flanking intron sequences were amplified by PCR and direct sequencing of the PCR products was performed. Results This analysis revealed that besides the presence of some single nucleotide polymorphisms in the 3pk gene, we could not detect any possible loss of function mutation in any of these 30 metastatic melanoma samples selected for the presence of activating mutations within the Ras/Raf/Mek/Erk signaling pathway. Conclusion Hence, in melanoma with constitutively active Ras/Raf inactivating mutations within the 3pk gene do not contribute to the oncogenic phenotype of this highly malignant tumor. 19. Near-infrared radiation absorption properties of covellite (CuS using first-principles calculations Directory of Open Access Journals (Sweden) Lihua Xiao 2016-08-01 Full Text Available First-principles density functional theory was used to investigate the electronic structure, optical properties and the origin of the near-infrared (NIR absorption of covellite (CuS. The calculated lattice constant and optical properties are found to be in reasonable agreement with experimental and theoretical findings. The electronic structure reveals that the valence and conduction bands of covellite are determined by the Cu 3d and S 3p states. By analyzing its optical properties, we can fully understand the potential of covellite (CuS as a NIR absorbing material. Our results show that covellite (CuS exhibits NIR absorption due to its metal-like plasma oscillation in the NIR range. 20. Near-infrared radiation absorption properties of covellite (CuS) using first-principles calculations Energy Technology Data Exchange (ETDEWEB) Xiao, Lihua, E-mail: xiaolihua@git.edu.cn [School of Materials and Metallurgical Engineering, Guizhou Institute of Technology, Guiyang 550003 (China); College of Physics and Information Science, Hunan Normal University, Changsha 410081 (China); Guizhou Special Functional Materials 2011 Collaborative Innovation Center, Guizhou Institute of Technology, Guiyang 550003 (China); Wu, Jianming; Liu, Yike; Lu, Fanghai [School of Materials and Metallurgical Engineering, Guizhou Institute of Technology, Guiyang 550003 (China); Guizhou Special Functional Materials 2011 Collaborative Innovation Center, Guizhou Institute of Technology, Guiyang 550003 (China); Ran, Jingyu; Qiu, Wei; Shao, Fang [Guizhou Special Functional Materials 2011 Collaborative Innovation Center, Guizhou Institute of Technology, Guiyang 550003 (China); Tang, Dongsheng, E-mail: dstang@hunnu.edu.cn [College of Physics and Information Science, Hunan Normal University, Changsha 410081 (China); Peng, Ping [School of Materials Science and Engineering, Hunan University, Changsha, Hunan 410082 (China) 2016-08-15 First-principles density functional theory was used to investigate the electronic structure, optical properties and the origin of the near-infrared (NIR) absorption of covellite (CuS). The calculated lattice constant and optical properties are found to be in reasonable agreement with experimental and theoretical findings. The electronic structure reveals that the valence and conduction bands of covellite are determined by the Cu 3d and S 3p states. By analyzing its optical properties, we can fully understand the potential of covellite (CuS) as a NIR absorbing material. Our results show that covellite (CuS) exhibits NIR absorption due to its metal-like plasma oscillation in the NIR range. 1. Magnetic Moment of ^{59}Cu CERN Multimedia 2002-01-01 Experiment IS358 uses the intense and pure beams of copper isotopes provided by the ISOLDE RILIS (resonance ionization laser ion source). The isotopes are implanted and oriented in the low temperature nuclear orientation set-up NICOLE. Magnetic moments are measured by \\beta-NMR. Copper (Z=29), with a single proton above the proton-magic nickel isotopes provides an ideal testground for precise shell model calculations of magnetic moments and their experimental verification. In the course of our experiments we already determined the magnetic moments of ^{67}Ni, ^{67}Cu, ^{68g}Cu, ^{69}Cu and ^{71}Cu which provide important information on the magicity of the N=40 subshell closure. In 2001 we plan to conclude our systematic investigations by measuring the magnetic moment of the neutron-deficient isotope ^{59}Cu. This will pave the way for a subsequent study of the magnetic moment of ^{57}Cu with a complementary method. 2. Reduced miR-659-3p levels correlate with progranulin increase in hypoxic conditions: implications for frontotemporal dementia. Directory of Open Access Journals (Sweden) Paola ePiscopo 2016-05-01 Full Text Available Progranulin (PGRN is a secreted protein expressed ubiquitously throughout the body, including the brain, where it localizes in neurons and activated microglia. Loss-of-function mutations in the GRN gene are an important cause of familial Frontotemporal Lobar Degeneration (FTLD. PGRN has a neurotrophic and anti-inflammatory activity, and it is neuroprotective in several injury conditions, such as oxygen or glucose deprivation, oxidative injury, and hypoxic stress. Indeed, we have previously demonstrated that hypoxia induces the up-regulation of GRN transcripts. Several studies have shown microRNAs involvement in hypoxia. Moreover, in FTLD patients with a genetic variant of GRN (rs5848, the reinforcement of miR-659-3p binding site has been suggested to be a risk factor. Here, we report that miR-659-3p interacts directly with GRN 3’UTR as shown by luciferase assay in HeLa cells and ELISA and Western Blot analysis in HeLa and Kelly cells. Moreover, we demonstrate the physical binding between GRN mRNA and miR-659-3p employing a miRNA capture-affinity technology in SK-N-BE and Kelly cells. In order to study miRNAs involvement in hypoxia-mediated up-regulation of GRN, we evaluated miR-659-3p levels in SK-N-BE cells after 24h of hypoxic treatment, finding them inversely correlated to GRN transcripts. Furthermore, we analyzed an animal model of asphyxia, finding that GRN mRNA levels increased at post-natal day (pnd 1 and pnd 4 in rat cortices subjected to asphyxia in comparison to control rats and miR-659-3p decreased at pnd 4 just when GRN reached the highest levels. Our results demonstrate the interaction between miR-659-3p and GRN transcript and the involvement of miR-659-3p in GRN up-regulation mediated by hypoxic/ischemic insults. 3. Radiosensitization effect of hsa-miR-138-2-3p on human laryngeal cancer stem cells Directory of Open Access Journals (Sweden) Ying Zhu 2017-05-01 Full Text Available Background Treatments that target cancer stem cells play an important role in the controlling and eliminating of tumor initiation as well as in development, progression, and chemotherapy/radiotherapy resistance. In our previous study, we cultured and harvested human laryngeal cancer stem cells (CSCs and applied microRNA biochips to screen differentially expressed miRNAs that were related to radiation tolerance in irradiated human laryngeal CSCs. According to the predicted genes and pathways of differential miRNAs target, down-regulated expression of hsa-miR-138-2-3p under radiation was thought to play a key role in enhancing the radio-sensitivity in human laryngeal squamous cancer stem cells. Method To investigate the radiational enhancement of hsa-miR-138-2-3p, we transfected hsa-miR-138-2-3p mimics that were synthesized based on the sequences of hsa-miR-138-2-3p in vitrointo human laryngeal CSCs (Hep-2, M2e, and TU212 cell lines to make hsa-miR-138-2-3p overexpressed, and the tumorous specialities of CSCs, like cell proliferation, invasion, apoptosis, cell cycle arrest, and DNA damage were evaluated by CCK-8 assay, clone formation assay, invasion assay, flow cytometry, and comet assay. Furthermore, we explored the signal transduction pathways that regulated the cancer stem cell initiation, development, invasion, apoptosis and cell cycle arrest, which were controlled by hsa-miR-138-2-3p. Result Overexpressed hsa-miR-138-2-3p played a key role in many anti-cancer biological processes in human laryngeal CSCs: (1 it decreased laryngeal CSCs proliferation and invasion in response to radiotherapy; (2 it increased the proportion of early and late apoptosis in laryngeal CSCs after radiation, raised G1 phase arrest in laryngeal CSCs after radiation, and decreased the proportion of S stage cells of cell cycle that were related to radio-resistance in laryngeal CSCs; (3 it down-regulated the expression of β-catenin in Wnt signal pathway that was related 4. MiR-466b-1-3p regulates P-glycoprotein expression in rat cerebral microvascular endothelial cells. Science.gov (United States) Yang, Xiaobo; Ren, Weimin; Shao, Yiye; Chen, Yinghui 2017-04-03 Epilepsy is one of the most common neurological disorders, and approximately one-third of epilepsy cases are resistant to treatment with anti-epileptic drug (AED). P-glycoprotein (P-gp) is a multi-drug transporter that is thought to play a pivotal role in multiple drug resistance (MDR) in epilepsy. The regulatory mechanism of P-gp remains largely unknown; however, recent studies have demonstrated that microRNAs (miRNAs) may regulate the chemo-resistance mediated by P-gp. This study investigated the effect of specific miRNAs that regulate P-gp expression in rat cerebral microvascular endothelial cells (RCMECs). Primary cultures of RCMECs were treated with phenobarbital (PB) at various concentrations to induce P-gp overexpression. MiRNA microarrays were used to investigate the expression profiles of miRNAs in the resistant RCMECs induced by PB and corresponding non-resistant cells. Our data demonstrated decreased miR-466b-1-3p expression in the resistant cells compared with the non-resistant cells. Moreover, the recombinant RNA of 466b-1-3p (mimic) and the artificial antisense RNA of miR-466b-1-3p (inhibitor) were constructed and transfected into resistant RCMECs. The expression and function of P-gp were measured by Western blotting, quantitative real-time polymerase chain reaction (qRT-PCR) and flow cytometry using rhodamine efflux. The mRNA and protein levels of P-gp increased as the concentration of PB increased, whereas miR-466b-1-3p levels decreased with increasing PB concentrations (Pp mimic down-regulated P-gp expression, whereas the miR-466b-1-3p inhibitor up-regulated P-gp expression (Pp may regulate PB-induced P-gp expression in RCMECs. 5. Excess of a Rassf1-targeting microRNA, miR-193a-3p, perturbs cell division fidelity. Science.gov (United States) Pruikkonen, Sofia; Kallio, Marko J 2017-05-23 Several microRNA (miRNA) molecules have emerged as important post-transcriptional regulators of tumour suppressor and oncogene expression. Ras association domain family member 1 (RASSF1) is a critical tumour suppressor that controls multiple aspects of cell proliferation such as cell cycle, cell division and apoptosis. The expression of RASSF1 is lost in a variety of cancers due to the promoter hypermethylation. miR-193a-3p was identified as a RASSF1-targeting miRNA by a dual screening approach. In cultured human cancer cells, immunoblotting, qRT-PCR, luciferase reporter assays, time-lapse microscopy and immunofluorescence methods were used to study the effects of excess miR-193a-3p on RASSF1 expression and cell division. Here, we report a new miRNA-mediated mechanism that regulates RASSF1 expression: miR-193a-3p binds directly to RASSF1-3'UTR and represses the mRNA and protein expression. In human cancer cells, excess of miR-193a-3p causes polyploidy through impairment of the Rassf1-Syntaxin 16 signalling pathway that is needed for completion of cytokinesis. In the next cell cycle the miR-193a-3p-overexpressing cells exhibit multipolar mitotic spindles, mitotic delay and elevated frequency of cell death. Our results suggest that besides epigenetic regulation, altered expression of specific miRNAs may contribute to the loss of Rassf1 in cancer cells and cause cell division errors. 6. Evidence that intramolecular interactions are involved in masking the activation domain of transcriptional activator Leu3p. Science.gov (United States) Wang, D; Hu, Y; Zheng, F; Zhou, K; Kohlhaw, G B 1997-08-01 The Leu3 protein of Saccharomyces cerevisiae regulates the expression of genes involved in branched chain amino acid biosynthesis and in ammonia assimilation. It is modulated by alpha-isopropylmalate, an intermediate in leucine biosynthesis. In the presence of alpha-isopropylmalate, Leu3p is a transcriptional activator. In the absence of the signal molecule, the activation domain is masked, and Leu3p acts as a repressor. The recent discovery that Leu3p retains its regulatory properties when expressed in mammalian cells (Guo, H., and Kohlhaw, G. B. (1996) FEBS Lett. 390, 191-195) suggests that masking and unmasking of the activation domain occur without the participation of auxiliary proteins. Here we present experimental support for this notion and address the mechanism of masking. We show that modulation of Leu3p is exceedingly sensitive to mutations in the activation domain. An activation domain double mutant (D872N/D874N; designated Leu3-dd) was constructed that has the characteristics of a permanently masked activator. Using separately expressed segments containing either the DNA binding domain-middle region or the activation domain of wild type Leu3p (or Leu3-dd) in a modified yeast two-hybrid system, we provide direct evidence for alpha-isopropylmalate-dependent interaction between these segments. Finally, we use the phenotype of Leu3-dd-containing cells (slow growth in the absence of added leucine) to select for suppressor mutations that map to the middle region of Leu3-dd. The properties of nine such suppressors further support the idea that masking is an intramolecular process and suggest a means for mapping the surface involved in masking. 7. Role of miR-222-3p in c-Src-Mediated Regulation of Osteoclastogenesis Directory of Open Access Journals (Sweden) Shinya Takigawa 2016-02-01 Full Text Available MicroRNAs (miRNAs are small non-coding RNAs that play a mostly post-transcriptional regulatory role in gene expression. Using RAW264.7 pre-osteoclast cells and genome-wide expression analysis, we identified a set of miRNAs that are involved in osteoclastogenesis. Based on in silico analysis, we specifically focused on miR-222-3p and evaluated its role in osteoclastogenesis. The results show that the inhibitor of miR-222-3p upregulated the mRNA levels of nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1 and tartrate-resistant acid phosphatase (TRAP, while its mimicking agent downregulated their mRNA levels. Western blot analysis showed that its inhibitor increased the protein levels of TRAP and cathepsin K, while its mimicking agent decreased their levels. Genome-wide mRNA expression analysis in the presence and absence of receptor activator of nuclear factor κ-B ligand (RANKL predicted c-Src as a potential regulatory target of miR-222-3p. Live cell imaging using a fluorescence resonance energy transfer (FRET technique revealed that miR-222-3p acted as an inhibitor of c-Src activity, and a partial silencing of c-Src suppressed RANKL-induced expression of TRAP and cathepsin K, as well as the number of multi-nucleated osteoclasts and their pit formation. Collectively, the study herein demonstrates that miR-222-3p serves as an inhibitor of osteoclastogenesis and c-Src mediates its inhibition of cathepsin K and TRAP. 8. MAP3S precipitation chemistry network: sixth periodic summary report (1982) Energy Technology Data Exchange (ETDEWEB) Rothert, J.E.; Dana, M.T. 1983-07-01 This report contains complete field and chemical data from the MAP3S Precipitation Chemistry Network for the year 1982. Included is an update on network status and a summary of the USGS blind sample analysis program and laboratory sample exchanges during 1982. The statistical summary is deferred to a forthcoming publication. 9. Observation of the bottomonium ground state in the decay Upsilon(3S)-->gammaetab. Science.gov (United States) Aubert, B; Bona, M; Karyotakis, Y; Lees, J P; Poireau, V; Prencipe, E; Prudent, X; Tisserand, V; Garra Tico, J; Grauges, E; Lopez, L; Palano, A; Pappagallo, M; Eigen, G; Stugu, B; Sun, L; Abrams, G S; Battaglia, M; Brown, D N; Cahn, R N; Jacobsen, R G; Kerth, L T; Kolomensky, Yu G; Lynch, G; Osipenkov, I L; Ronan, M T; Tackmann, K; Tanabe, T; Hawkes, C M; Soni, N; Watson, A T; Koch, H; Schroeder, T; Walker, D; Asgeirsson, D J; Fulsom, B G; Hearty, C; Mattison, T S; McKenna, J A; Barrett, M; Khan, A; Blinov, V E; Bukin, A D; Buzykaev, A R; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, K Yu; Bondioli, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M; Martin, E C; Stoker, D P; Abachi, S; Buchanan, C; Gary, J W; Liu, F; Long, O; Shen, B C; Vitug, G M; Yasin, Z; Zhang, L; Sharma, V; Campagnari, C; Hong, T M; Kovalskyi, D; Mazur, M A; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Martinez, A J; Schalk, T; Schumm, B A; Seiden, A; Wilson, M G; Winstrom, L O; Cheng, C H; Doll, D A; Echenard, B; Fang, F; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Andreassen, R; Mancinelli, G; Meadows, B T; Mishra, K; Sokoloff, M D; Bloom, P C; Ford, W T; Gaz, A; Hirschauer, J F; Nagel, M; Nauenberg, U; Smith, J G; Ulmer, K A; Wagner, S R; Ayad, R; Soffer, A; Toki, W H; Wilson, R J; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Karbach, M; Merkel, J; Petzold, A; Spaan, B; Wacker, K; Kobel, M J; Mader, W F; Nogowski, R; Schubert, K R; Schwierz, R; Volk, A; Bernard, D; Bonneaud, G R; Latour, E; Verderi, M; Clark, P J; Playfer, S; Watson, J E; Andreotti, M; Bettoni, D; Bozzi, C; Calabrese, R; Cecchi, A; Cibinetto, G; Franchini, P; Luppi, E; Negrini, M; Petrella, A; Piemontese, L; Santoro, V; Baldini-Ferroli, R; Calcaterra, A; de Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzo, A; Contri, R; Lo Vetere, M; Macri, M M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Chaisanguanthum, K S; Morii, M; Adametz, A; Marks, J; Schenk, S; Uwer, U; Klose, V; Lacker, H M; Bard, D J; Dauncey, P D; Nash, J A; Tibbetts, M; Behera, P K; Chai, X; Charles, M J; Mallik, U; Cochran, J; Crawley, H B; Dong, L; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Gao, Y Y; Gritsan, A V; Guo, Z J; Lae, C K; Arnaud, N; Béquilleux, J; D'Orazio, A; Davier, M; da Costa, J Firmino; Grosdidier, G; Höcker, A; Lepeltier, V; Le Diberder, F; Lutz, A M; Pruvot, S; Roudeau, P; Schune, M H; Serrano, J; Sordini, V; Stocchi, A; Wormser, G; Lange, D J; Wright, D M; Bingham, I; Burke, J P; Chavez, C A; Fry, J R; Gabathuler, E; Gamet, R; Hutchcroft, D E; Payne, D J; Touramanis, C; Bevan, A J; Clarke, C K; George, K A; Di Lodovico, F; Sacco, R; Sigamani, M; Cowan, G; Flaecher, H U; Hopkins, D A; Paramesvaran, S; Salvatore, F; Wren, A C; Brown, D N; Davis, C L; Denig, A G; Fritsch, M; Gradl, W; Schott, G; Alwyn, K E; Bailey, D; Barlow, R J; Chia, Y M; Edgar, C L; Jackson, G; Lafferty, G D; West, T J; Yi, J I; Anderson, J; Chen, C; Jawahery, A; Roberts, D A; Simi, G; Tuggle, J M; Dallapiccola, C; Li, X; Salvati, E; Saremi, S; Cowan, R; Dujmic, D; Fisher, P H; Sciolla, G; Spitznagel, M; Taylor, F; Yamamoto, R K; Zhao, M; Patel, P M; Robertson, S H; Lazzaro, A; Lombardo, V; Palombo, F; Bauer, J M; Cremaldi, L; Godang, R; Kroeger, R; Sanders, D A; Summers, D J; Zhao, H W; Simard, M; Taras, P; Viaud, F B; Nicholson, H; De Nardo, G; Lista, L; Monorchio, D; Onorato, G; Sciacca, C; Raven, G; Snoek, H L; Jessop, C P; Knoepfel, K J; LoSecco, J M; Wang, W F; Benelli, G; Corwin, L A; Honscheid, K; Kagan, H; Kass, R; Morris, J P; Rahimi, A M; Regensburger, J J; Sekula, S J; Wong, Q K; Blount, N L; Brau, J; Frey, R; Igonkina, O; Kolb, J A; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Castelli, G; Gagliardi, N; Margoni, M; Morandin, M; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; del Amo Sanchez, P; Ben-Haim, E; Briand, H; Calderini, G; Chauveau, J; David, P; Del Buono, L; Hamon, O; Leruste, Ph; Ocariz, J; Perez, A; Prendki, J; Sitt, S; Gladney, L; Biasini, M; Covarelli, R; Manoni, E; Angelini, C; Batignani, G; Bettarini, S; Carpinelli, M; Cervelli, A; Forti, F; Giorgi, M A; Lusiani, A; Marchiori, G; Morganti, M; Neri, N; Paoloni, E; Rizzo, G; Walsh, J J; Lopes Pegna, D; Lu, C; Olsen, J; Smith, A J S; Telnov, A V; Anulli, F; Baracchini, E; Cavoto, G; del Re, D; Di Marco, E; Faccini, R; Ferrarotto, F; Ferroni, F; Gaspero, M; Jackson, P D; Gioi, L Li; Mazzoni, M A; Morganti, S; Piredda, G; Polci, F; Renga, F; Voena, C; Ebert, M; Hartmann, T; Schröder, H; Waldi, R; Adye, T; Franek, B; Olaiya, E O; Wilson, F F; Emery, S; Escalier, M; Esteve, L; Ganzhur, S F; de Monchenault, G Hamel; Kozanecki, W; Vasseur, G; Yèche, Ch; Zito, M; Chen, X R; Liu, H; Park, W; Purohit, M V; White, R M; Wilson, J R; Allen, M T; Aston, D; Bartoldus, R; Bechtle, P; Benitez, J F; Bertsche, K; Cai, Y; Cenci, R; Coleman, J P; Convery, M R; Decker, F J; Dingfelder, J C; Dorfan, J; Dubois-Felsmann, G P; Dunwoodie, W; Ecklund, S; Erickson, R; Field, R C; Fisher, A; Fox, J; Gabareen, A M; Gowdy, S J; Graham, M T; Grenier, P; Hast, C; Innes, W R; Iverson, R; Kaminski, J; Kelsey, M H; Kim, H; Kim, P; Kocian, M L; Kulikov, A; Leith, D W G S; Li, S; Lindquist, B; Luitz, S; Luth, V; Lynch, H L; Macfarlane, D B; Marsiske, H; Messner, R; Muller, D R; Neal, H; Nelson, S; Novokhatski, A; O'Grady, C P; Ofte, I; Perazzo, A; Perl, M; Ratcliff, B N; Rivetta, C; Roodman, A; Salnikov, A A; Schindler, R H; Schwiening, J; Seeman, J; Snyder, A; Su, D; Sullivan, M K; Suzuki, K; Swain, S K; Thompson, J M; Va'vra, J; Van Winkle, D; Wagner, A P; Weaver, M; West, C A; Wienands, U; Wisniewski, W J; Wittgen, M; Wittmer, W; Wright, D H; Wulsin, H W; Yan, Y; Yarritu, A K; Yi, K; Yocky, G; Young, C C; Ziegler, V; Burchat, P R; Edwards, A J; Majewski, S A; Miyashita, T S; Petersen, B A; Wilden, L; Ahmed, S; Alam, M S; Ernst, J A; Pan, B; Saeed, M A; Zain, S B; Spanier, S M; Wogsland, B J; Eckmann, R; Ritchie, J L; Ruland, A M; Schilling, C J; Schwitters, R F; Drummond, B W; Izen, J M; Lou, X C; Bianchi, F; Gamba, D; Pelliccioni, M; Bomben, M; Bosisio, L; Cartaro, C; Della Ricca, G; Lanceri, L; Vitale, L; Azzolini, V; Lopez-March, N; Martinez-Vidal, F; Milanes, D A; Oyanguren, A; Albert, J; Banerjee, Sw; Bhuyan, B; Choi, H H F; Hamano, K; Kowalewski, R; Lewczuk, M J; Nugent, I M; Roney, J M; Sobie, R J; Gershon, T J; Harrison, P F; Ilic, J; Latham, T E; Mohanty, G B; Band, H R; Chen, X; Dasu, S; Flood, K T; Pan, Y; Pierini, M; Prepost, R; Vuosalo, C O; Wu, S L 2008-08-15 We report the results of a search for the bottomonium ground state etab(1S) in the photon energy spectrum with a sample of (109+/-1) million of Upsilon(3S) recorded at the Upsilon(3S) energy with the BABAR detector at the PEP-II B factory at SLAC. We observe a peak in the photon energy spectrum at Egamma=921.2(-2.8)+2.1(stat)+/-2.4(syst) MeV with a significance of 10 standard deviations. We interpret the observed peak as being due to monochromatic photons from the radiative transition Upsilon(3S)-->gammaetab(1S). This photon energy corresponds to an etab(1S) mass of 9388.9(-2.3)+3.1(stat)+/-2.7(syst) MeV/c2. The hyperfine Upsilon(1S)-etab(1S) mass splitting is 71.4(-3.1)+2.3(stat)+/-2.7(syst) MeV/c2. The branching fraction for this radiative Upsilon(3S) decay is estimated to be [4.8+/-0.5(stat)+/-1.2(syst)]x10(-4). 10. Sma3s: A Three-Step Modular Annotator for Large Sequence Datasets Science.gov (United States) Muñoz-Mérida, Antonio; Viguera, Enrique; Claros, M. Gonzalo; Trelles, Oswaldo; Pérez-Pulido, Antonio J. 2014-01-01 Automatic sequence annotation is an essential component of modern ‘omics’ studies, which aim to extract information from large collections of sequence data. Most existing tools use sequence homology to establish evolutionary relationships and assign putative functions to sequences. However, it can be difficult to define a similarity threshold that achieves sufficient coverage without sacrificing annotation quality. Defining the correct configuration is critical and can be challenging for non-specialist users. Thus, the development of robust automatic annotation techniques that generate high-quality annotations without needing expert knowledge would be very valuable for the research community. We present Sma3s, a tool for automatically annotating very large collections of biological sequences from any kind of gene library or genome. Sma3s is composed of three modules that progressively annotate query sequences using either: (i) very similar homologues, (ii) orthologous sequences or (iii) terms enriched in groups of homologous sequences. We trained the system using several random sets of known sequences, demonstrating average sensitivity and specificity values of ∼85%. In conclusion, Sma3s is a versatile tool for high-throughput annotation of a wide variety of sequence datasets that outperforms the accuracy of other well-established annotation algorithms, and it can enrich existing database annotations and uncover previously hidden features. Importantly, Sma3s has already been used in the functional annotation of two published transcriptomes. PMID:24501397 11. LiBi3S5-A lithium bismuth sulfide with strong cation disorder Science.gov (United States) Nakhal, Suliman; Wiedemann, Dennis; Stanje, Bernhard; Dolotko, Oleksandr; Wilkening, Martin; Lerch, Martin 2016-06-01 Among chalcogenide semiconductors for thermoelectric applications, alkali-metal bismuth compounds occur in many complex compositions favorable for high performance. Although LiBi3S5 had been announced in 1977, the potential 1D lithium-ion conductor has hitherto eluded selective synthesis and structure determination. In this study, we present a solid-state route to phase-pure LiBi3S5 powder starting from LiBiS2 and Bi2S3. Neutron diffractograms and lithium NMR spectra reveal its crystal structure to be a cation-disordered variety of the AgBi3S5 type (synthetic pavonite; monoclinic, C2/m). Topological analyses and lithium NMR relaxometry suggest that correlated lithium-ion diffusion with activation energies up to 0.66(2) eV occurs along the channels in b direction including tetrahedral voids. Because of cation disorder, immobile bismuth(III) ions clog these pathways, making LiBi3S5 a moderate to poor ionic conductor. The synthesis route reported is nonetheless promising for new lithium bismuth sulfides with, possibly ordered, structure types of the pavonite homologous series. 12. Hydrogen Evolution on Supported Incomplete Cubane-type [Mo3S4](4+) Electrocatalysts DEFF Research Database (Denmark) Jaramillo, Thomas; Bonde, Jacob Lindner; Zhang, Jingdong 2008-01-01 Electrocatalytic properties of biomimetic supported incomplete cubane-type [Mo3S4](4+) clusters are investigated. The activity toward the hydrogen evolution reaction (HER) is evaluated on both a high surface area gas diffusion electrode in a membrane electrode assembly and on highly orientated... 13. Methods of using (1S,3S)-3-amino-4-difluoromethylenyl-1-cyclopentanoic acid Energy Technology Data Exchange (ETDEWEB) Silverman, Richard B; Dewey, Stephen L; Miller, Steven 2015-03-03 (1S,3S)-3-amino-4-difluoromethylenyl-1-cyclopentanoic acid also known as CPP-115 or its pharmaceutically acceptable salts can be used to treat addiction and neurological disorders such as epilepsy without side effects such as visual field defects caused by vigabatrin (Sabril). 14. The Rapid Response Radiation Survey (R3S) Mission Using the HISat Conformal Satellite Architecture Science.gov (United States) Miller, Nathanael 2015-01-01 The Rapid Response Radiation Survey (R3S) experiment, designed as a quick turnaround mission to make radiation measurements in LEO, will fly as a hosted payload in partnership with NovaWurks using their Hyper-integrated Satlet (HiSat) architecture. The need for the mission arises as the Nowcast of Atmospheric Ionization Radiation for Aviation Safety (NAIRAS) model moves from a research effort into an operational radiation assessment tool. The data collected by R3S, in addition to the complementary data from a NASA Langley Research Center (LaRC) atmospheric balloon mission entitled Radiation Dosimetry Experiment (RaDX), will validate exposure prediction capabilities of NAIRAS. This paper discusses the development of the R3S experiment as made possible by use of the HiSat architecture. The system design and operational modes of the experiment are described, as well as the experiment interfaces to the HiSat satellite via the user defined adapter (UDA) provided by NovaWurks. This paper outlines the steps taken by the project to execute the R3S mission in the 4 months of design, build, and test. Finally, description of the engineering process is provided, including the use of facilitated rapid/concurrent engineering sessions, the associated documentation, and the review process employed. 15. The MAP3S precipitation chemistry network: Eighth periodic summary report (1984) Energy Technology Data Exchange (ETDEWEB) Dana, M.T. 1987-01-01 This summary report, the eighth in the series, contains complete field and chemical data from the MAP3S Precipitation Chemistry Network for the year 1984. Sections cover Quality Assurance/Quality Control, network site information, and a brief statistical summary of the 1984 data. 16. High-Purity Fe3S4 Greigite Microcrystals for Magnetic and Electrochemical Performance NARCIS (Netherlands) Li, Guowei; Zhang, Baomin; Yu, Feng; Novakova, Alla A.; Krivenkov, Maxim S.; Kiseleva, Tatiana Y.; Chang, Liao; Rao, Jiancun; Polyakov, Alexey O.; Blake, Graeme R.; de Groot, Robert A.; Palstra, Thomas T. M. 2014-01-01 High-purity Fe3S4 (greigite) microcrystals with octahedral shape were synthesized via a simple hydrothermal method using a surfactant. The as-prepared samples have the inverse spinel structure with high crystallinity. The saturation magnetization (M-s) reaches 3.74 mu(B) at 5 K and 3.51 mu(B) at roo 17. Modification of chimeric (2S, 3S)-butanediol dehydrogenase based on structural information. Science.gov (United States) Shimegi, Tomohito; Mochizuki, Kaito; Oyama, Takuji; Ohtsuki, Takashi; Kusunoki, Masami; Ui, Sadaharu 2014-01-01 A chimeric (2S, 3S)-butanediol dehydrogenase (cLBDH) was engineered to have the strict (S)-configuration specificity of the (2S, 3S)-BDH (BsLBDH) derived from Brevibacterium saccharolyticum as well as the enzymatic stability of the (2R, 3S)-BDH (KpMBDH) from Klebsiella pneumonia by swapping the domains of two native BDHs. However, while cLBDH possesses the stability, it lacks the specificity. In order to assist in the design a BDH having strict substrate specificity, an X-ray structural analysis of a cLBDH crystal was conducted at 1.58 Å. The results obtained show some readily apparent differences around the active sites of cLBDH and BsLBDH. Based on this structural information, a novel (2S, 3S)-BDH having a preferred specificity was developed by introducing a V254L mutation into cLBDH. The influence of this mutation on the stability of cLBDH was not evaluated. Nevertheless, the technique described herein is an effective method for the production of a tailor-made BDH. 18. Ionic liquid-modulated synthesis of ferrimagnetic Fe(3)S(4) hierarchical superstructures. Science.gov (United States) Ma, Jianmin; Chang, Liao; Lian, Jiabiao; Huang, Zheng; Duan, Xiaochuan; Liu, Xiaodi; Peng, Peng; Kim, Tongil; Liu, Zhifang; Zheng, Wenjun 2010-07-21 Ferrimagnetic Fe(3)S(4) nanowalls and triple hierarchical microspheres have been fabricated via an ionic liquid-modulated solution-phase process, respectively. Magnetic hysteresis measurements demonstrate that their morphology-dependent magnetic properties might be originated from their unique nanostructures. 19. "IT-planeet" - kontakt tulnukatega TV 3s / Annely Adermann ; kommenteerinud Tarmo Randel Index Scriptorium Estoniae Adermann, Annely, 1974- 2011-01-01 Uuest hariduslikust teleseriaalist "IT-planeet", mille eesmärk on vaatajale selgitada arvutite ja turvalisusega seotud teemasid. Režissöör Margus Paju, stsenarist Rasmus Merivoo. Seriaali näeb TV 3-s alates 16. märtsist 20. Morphology-controllable synthesis of CuO nanostructures and their catalytic activity for the reduction of 4-nitrophenol Science.gov (United States) Che, Wei; Ni, Yonghong; Zhang, Yuxing; Ma, Yue 2015-02-01 The investigation on the correlation between properties and shapes of nanomaterials always draws increased interest. However, the correlation between properties and shapes of CuO nanostructures was rarely reported in the previous works. The shape-controlled preparation of CuO nanostructures was successfully realized in the present work via a simple oil-bath route in air at 170 °;C for 30 min, employing CuCl2•2H2O and NaOH as the reactants. It was found that CuO nanocrystals with leaf-like, dumbbell-like and flowerlike structures were obtained through introducing various additives. At the same time, the correlation between properties and shapes was investigated. It was found that the catalytic performances of the as-prepared CuO nanostructures for the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in NaBH4 aqueous solution were dependent on its morphologies. Experiments showed that the first-order rate constants for CuO nanostructures with various shapes were in turn 35.5‧ 10-3 s-1 (leaf-like nanosheets), 4.77‧ 10-3 s-1 (dumbbell-like architectures), 10.6‧ 10-3 s-1 (flowerlike nanostructures). The present research provides a new catalyst selection for the reduction of 4-NP to 4-AP in excess NaBH4 aqueous solution, which has potential application in industrial production. 1. MicroRNAs miR-203-3p, miR-664-3p and miR-708-5p are associated with median strain lifespan in mice Science.gov (United States) Lee, Benjamin P.; Burić, Ivana; George-Pandeth, Anupriya; Flurkey, Kevin; Harrison, David E.; Yuan, Rong; Peters, Luanne L.; Kuchel, George A.; Melzer, David; Harries, Lorna W. 2017-01-01 MicroRNAs (miRNAs) are small non-coding RNA species that have been shown to have roles in multiple processes that occur in higher eukaryotes. They act by binding to specific sequences in the 3’ untranslated region of their target genes and causing the transcripts to be degraded by the RNA-induced silencing complex (RISC). MicroRNAs have previously been reported to demonstrate altered expression in several aging phenotypes such as cellular senescence and age itself. Here, we have measured the expression levels of 521 small regulatory microRNAs (miRNAs) in spleen tissue from young and old animals of 6 mouse strains with different median strain lifespans by quantitative real-time PCR. Expression levels of 3 microRNAs were robustly associated with strain lifespan, after correction for multiple statistical testing (miR-203-3p [β-coefficient = −0.6447, p = 4.8 × 10−11], miR-664-3p [β-coefficient = 0.5552, p = 5.1 × 10−8] and miR-708-5p [β-coefficient = 0.4986, p = 1.6 × 10−6]). Pathway analysis of binding sites for these three microRNAs revealed enrichment of target genes involved in key aging and longevity pathways including mTOR, FOXO and MAPK, most of which also demonstrated associations with longevity. Our results suggests that miR-203-3p, miR-664-3p and miR-708-5p may be implicated in pathways determining lifespan in mammals. PMID:28304372 2. File list: His.PSC.05.H3S10ph.AllCell [Chip-atlas[Archive Lifescience Database Archive (English) Full Text Available His.PSC.05.H3S10ph.AllCell mm9 Histone H3S10ph Pluripotent stem cell SRX963273,SRX9...63272 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.05.H3S10ph.AllCell.bed ... 3. Polymeric nanoparticle-based delivery of microRNA-199a-3p inhibits proliferation and growth of osteosarcoma cells Directory of Open Access Journals (Sweden) Zhang L 2015-04-01 Full Text Available Linlin Zhang,1,2,* Arun K lyer,3,4,* Xiaoqian Yang,1 Eisuke Kobayashi,1 Yuqi Guo,1,2 Henry Mankin,1 Francis J Hornicek,1 Mansoor M Amiji,3 Zhenfeng Duan1 1Sarcoma Biology Laboratory, Center for Sarcoma and Connective Tissue Oncology, Massachusetts General Hospital, Boston, Massachusetts, USA; 2Department of Pathology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China; 3Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University, Boston, Massachusetts, USA; 4Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA *These authors contributed equally to this work Abstract: Our prior screening of microRNAs (miRs identified that miR-199a-3p expression is reduced in osteosarcoma cells, one of the most common types of bone tumor. miR-199a-3p exhibited functions of tumor cell growth inhibition, suggesting the potential application of miR-199a-3p as an anticancer agent. In the study reported here, we designed and developed a lipid-modified dextran-based polymeric nanoparticle platform for encapsulation of miRs, and determined the efficiency and efficacy of delivering miR-199a-3p into osteosarcoma cells. In addition, another potent miR, let-7a, which also displayed tumor suppressive ability, was selected as a candidate miR for evaluation. Fluorescence microscopy studies and real-time polymerase chain reaction results showed that dextran nanoparticles could deliver both miR-199a-3p and let-7a into osteosarcoma cell lines (KHOS and U-2OS successfully. Western blotting analysis and 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assays demonstrated that dextran nanoparticles loaded with miRs could efficiently downregulate the expression of target proteins and effectively inhibit the growth and proliferation of osteosarcoma cells. These results demonstrate that a lipid-modified dextran 4. Effect of CuO on the Formation Mechanism of Calcium Sulphoaluminate Institute of Scientific and Technical Information of China (English) MA Suhua; SHEN Xiaohong; HUANG Yeping; ZHONG Baiqian 2008-01-01 Calcium sulphoaluminate was prepared with chemical reagents in this paper.The formation mechanism of calcium sulphoaluminate and effect of CuO on the formation mechanism of calcium sulphoaluminate were investigated by the chemical analysis,X-ray diffraction(XRD),and differential scanning calorimetry(DSC).The results show that there are three ways in the formation of calcium sulphoaluminate.CuO can promote the decomposition of calcium carbonate and decrease the formation temperature of calcium sulphoaluminate(C4A3S).When the burning temperature is below 1000℃,the addition of CuO can promote the formation of calcium sulphoaluminate,while CuO can not favor the formation of calcium sulphoaluminate above 1000℃. 5. Unexpected strong magnetism of Cu doped single-layer MoS₂ and its origin. Science.gov (United States) Yun, Won Seok; Lee, J D 2014-05-21 The magnetism of the 3d transition-metal (TM) doped single-layer (1L) MoS2, where the Mo atom is partially replaced by the 3d TM atom, is investigated using the first-principles density functional calculations. In a series of 3d TM doped 1L-MoS2's, the induced spin polarizations are negligible for Sc, Ti, and Cr dopings, while the induced spin polarizations are confirmed for V, Mn, Fe, Co, Ni, Cu, and Zn dopings and the systems become magnetic. Especially, the Cu doped system shows unexpectedly strong magnetism although Cu is nonmagnetic in its bulk state. The driving force is found to be a strong hybridization between Cu 3d states and 3p states of neighboring S, which results in an extreme unbalanced spin-population in the spin-split impurity bands near the Fermi level. Finally, we also discuss further issues of the Cu induced magnetism of 1L-MoS2 such as investigation of additional charge states, the Cu doping at the S site instead of the Mo site, and the Cu adatom on the layer (i.e., 1L-MoS2). 6. Sulfur donor atom effects on copper(I)/O(2) chemistry with thioanisole containing tetradentate N(3)S ligand leading to μ-1,2-peroxo-dicopper(II) species. Science.gov (United States) Lee, Yunho; Lee, Dong-Heon; Park, Ga Young; Lucas, Heather R; Narducci Sarjeant, Amy A; Kieber-Emmons, Matthew T; Vance, Michael A; Milligan, Ashley E; Solomon, Edward I; Karlin, Kenneth D 2010-10-04 To better understand the effect of thioether coordination in copper-O(2) chemistry, the tetradentate N(3)S ligand L(ASM) (2-(methylthio)-N,N-bis((pyridin-2-yl)methyl)benzenamine) and related alkylether ligand L(EOE) (2-ethoxy-N,N-bis((pyridin-2-yl)methyl)ethanamine) have been studied. The corresponding copper(I) complexes, [(L(ASM))Cu(I)](+) (1a) and [(L(EOE))Cu(I)](+) (3a), were studied as were the related compound [(L(ESE))Cu(I)](+) (2a, L(ESE) = (2-ethylthio-N,N-bis((pyridin-2-yl)methyl)ethanamine). The X-ray structure of 1a and its solution conductivity reveal a monomeric molecular structure possessing thioether coordination which persists in solution. In contrast, the C-O stretching frequencies of the derivative Cu(I)-CO complexes reveal that for these complexes, the modulated ligand arms, whether arylthioether, alkylthioether, or ether, are not coordinated to the cuprous ion. Electrochemical data for 1a and 2a in CH(3)CN and N,N-dimethylformamide (DMF) show the thioanisole moiety to be a poor electron donor compared to alkylthioether (1a is ∼200 mV more positive than 2a). The structures of [(L(ASM))Cu(II)(CH(3)OH)](2+) (1c) and [(L(ESE))Cu(II)(CH(3)OH)](2+) (2c) have also been obtained and indicate nearly identical copper coordination environments. Oxygenation of 1a at reduced temperature gives a characteristic deep blue intermediate [{(L(ASM))Cu(II)}(2)(O(2)(2-))](2+) (1b(P)) with absorption features at 442 (1,500 M(-1) cm(-1)), 530 (8,600 M(-1) cm(-1)), and 605 nm (10,400 M(-1) cm(-1)); these values compare well to the ligand-to-metal charge-transfer (LMCT) transitions previously reported for [{(L(ESE))Cu(II)}(2)(O(2)(2-))](2+) (2b(P)). Resonance Raman data for [{(L(ASM))Cu(II)}(2)(O(2)(2-))](2+) (1b(P)) support the formation of μ-1,2-peroxo species ν(O-O) = 828 cm(-1)(Δ((18)O(2)) = 48), ν(sym)(Cu-O) = 547 cm(-1) (Δ((18)O(2)) = 23), and ν(asym)(Cu-O) = 497 cm(-1) (Δ((18)O(2)) = 22) and suggest the L(ASM) ligand is a poorer electron donor to copper 7. Photoassociation spectroscopy of long-range molecular states below the 2s + 3p 6Li2 asymptote Science.gov (United States) Gross, Christian; Chaudhuri, Saptarishi; Gan, Jaren; Dieckmann, Kai 2015-05-01 We present photoassociation spectra of high-lying vibrational states of the interatomic potentials correlating to the 2s + 3p asymptote of 6Li2 . Starting from an atomic cloud in a magneto-optical trap we first drive a free-to-bound transition into a molecular bound state using a tunable ultra-violet laser. Thereafter we ionize these long-range molecules using a 532 nm laser and detect the resulting ions with a channeltron. We determine the absolute positions of the transitions with MHz precision utilizing a frequency comb based calibration. Lithium dimers are extensively studied theoretically using various models and methods. Spectroscopic measurements are crucial to test and benchmark these methods and are available for various electronic states and inter-nuclear distances of 6Li2 molecule. Our study provides the first experimental observation of long-range states of the 2s + 3p asymptote of 6Li2 . 8. Proton collisional excitation in the lowest lying 3P terms of ions in the Be and Mg isoelectronic sequences Science.gov (United States) Landman, D. A.; Brown, T. 1979-01-01 Proton collisional excitation cross sections and rate constants are presented for transitions between the 3P(J) fine-structure levels of the lowest-lying sp configurations in a number of astrophysically important ions belonging to the Be and Mg isoelectronic sequences. The calculations were made by direct integration of the Schroedinger equation resulting from semiclassical Coulomb excitation theory. The cross sections and rate constants for the 3P(J) transitions in the lowest-lying P(2) configurations are expected to be similar to those for the corresponding sp configuration transitions, and this is illustrated for C III. For the high-temperature ion Ca XVII alpha particle excitation is shown to be unimportant for situations involving ordinary values of the He/H abundance ratio. A simple, but apparently accurate method for determining certain radial integrals for low-lying excited configurations is proposed. 9. Efficient transfer hydrogenation reaction Catalyzed by a dearomatized PN 3P ruthenium pincer complex under base-free Conditions KAUST Repository He, Lipeng 2012-03-01 A dearomatized complex [RuH(PN 3P)(CO)] (PN 3PN, N′-bis(di-tert-butylphosphino)-2,6-diaminopyridine) (3) was prepared by reaction of the aromatic complex [RuH(Cl)(PN 3P)(CO)] (2) with t-BuOK in THF. Further treatment of 3 with formic acid led to the formation of a rearomatized complex (4). These new complexes were fully characterized and the molecular structure of complex 4 was further confirmed by X-ray crystallography. In complex 4, a distorted square-pyramidal geometry around the ruthenium center was observed, with the CO ligand trans to the pyridinic nitrogen atom and the hydride located in the apical position. The dearomatized complex 3 displays efficient catalytic activity for hydrogen transfer of ketones in isopropanol. © 2011 Elsevier B.V. All rights reserved. 10. A software platform to analyse the ethical issues of electronic patient privacy policy: the S3P example. Science.gov (United States) Mizani, M A; Baykal, N 2007-12-01 Paper-based privacy policies fail to resolve the new changes posed by electronic healthcare. Protecting patient privacy through electronic systems has become a serious concern and is the subject of several recent studies. The shift towards an electronic privacy policy introduces new ethical challenges that cannot be solved merely by technical measures. Structured Patient Privacy Policy (S3P) is a software tool assuming an automated electronic privacy policy in an electronic healthcare setting. It is designed to simulate different access levels and rights of various professionals involved in healthcare in order to assess the emerging ethical problems. The authors discuss ethical issues concerning electronic patient privacy policies that have become apparent during the development and application of S3P. 11. Beta decay of Cu-56 NARCIS (Netherlands) Borcea, R; Aysto, J; Caurier, E; Dendooven, P; Doring, J; Gierlik, M; Gorska, M; Grawe, H; Hellstrom, M; Janas, Z; Jokinen, A; Karny, M; Kirchner, R; La Commara, M; Langanke, K; Martinez-Pinedo, G; Mayet, P; Nieminen, A; Nowacki, F; Penttila, H; Plochocki, A; Rejmund, M; Roeckl, E; Schlegel, C; Schmidt, K; Schwengner, R; Sawicka, M 2001-01-01 The proton-rich isotope Cu-56 was produced at the GSI On-Line Mass Separator by means of the Si-28(S-32, p3n) fusion-evaporation reaction. Its beta -decay properties were studied by detecting beta -delayed gamma rays and protons. A half-Life of 93 +/- 3 ms was determined for Cu-56. Compared to the p 12. Clinical significance of circulating miR-25-3p as a novel diagnostic and prognostic biomarker in osteosarcoma Science.gov (United States) Fujiwara, Tomohiro; Uotani, Koji; Yoshida, Aki; Morita, Takuya; Nezu, Yutaka; Kobayashi, Eisuke; Yoshida, Akihiko; Uehara, Takenori; Omori, Toshinori; Sugiu, Kazuhisa; Komatsubara, Tadashi; Takeda, Ken; Kunisada, Toshiyuki; Kawamura, Machiko; Kawai, Akira; Ochiya, Takahiro; Ozaki, Toshifumi 2017-01-01 Background Emerging evidence has suggested that circulating microRNAs (miRNAs) in body fluids have novel diagnostic and prognostic significance for patients with malignant diseases. The lack of useful biomarkers is a crucial problem of bone and soft tissue sarcomas; therefore, we investigated the circulating miRNA signature and its clinical relevance in osteosarcoma. Methods Global miRNA profiling was performed using patient serum collected from a discovery cohort of osteosarcoma patients and controls and cell culture media. The secretion of the detected miRNAs from osteosarcoma cells and clinical relevance of serum miRNA levels were evaluated using in vitro and in vivo models and a validation patient cohort. Results Discovery screening identified 236 serum miRNAs that were highly expressed in osteosarcoma patients compared with controls, and eight among these were also identified in the cell culture media. Upregulated expression levels of miR-17-5p and miR-25-3p were identified in osteosarcoma cells, and these were abundantly secreted into the culture media in tumor-derived exosomes. Serum miR-25-3p levels were significantly higher in osteosarcoma patients than in control individuals in the validation cohort, with favorable sensitivity and specificity compared with serum alkaline phosphatase. Furthermore, serum miR-25-3p levels at diagnosis were correlated with patient prognosis and reflected tumor burden in both in vivo models and patients; these associations were more sensitive than those of serum alkaline phosphatase. Conclusions Serum-based circulating miR-25-3p may serve as a non-invasive blood-based biomarker for tumor monitoring and prognostic prediction in osteosarcoma patients. PMID:28380419 13. Stationary and dispersive features in resonant inelastic soft X-ray scattering at the Ge 3p resonances Energy Technology Data Exchange (ETDEWEB) Glover, C.J. [Department of Electronic Materials Engineering, Australian National University, Canberra, ACT 0200 (Australia); Schmitt, T. [SLS, Paul Scherrer Institut, PSI West, CH-5232 Villigen PSI (Switzerland); Mattesini, M. [Departamento de Fisica de la Tierra, Astronomia y Astrofisica I, Universidad Complutense de Madrid, E-28040 (Spain); Adell, M.; Ilver, L.; Kanski, J. [Chalmers University of Technology, SE-412 96 Goeteborg (Sweden); Kjeldgaard, L. [MAX-lab, Lund University, P.O. Box 118, SE-22100 Lund (Sweden); Agaker, M. [Department of Physics and Materials Science, Uppsala University, PO Box 530, SE-751 21 Uppsala (Sweden); Martensson, N. [MAX-lab, Lund University, P.O. Box 118, SE-22100 Lund (Sweden); Department of Physics and Materials Science, Uppsala University, PO Box 530, SE-751 21 Uppsala (Sweden); Ahuja, R.; Nordgren, J. [Department of Physics and Materials Science, Uppsala University, PO Box 530, SE-751 21 Uppsala (Sweden); Rubensson, J.-E. [Department of Physics and Materials Science, Uppsala University, PO Box 530, SE-751 21 Uppsala (Sweden)], E-mail: jan-erik.rubensson@fysik.uu.se 2009-07-15 Resonant inelastic soft X-ray scattering at the 3p resonances in crystalline Ge is presented. Both stationary and dispersive features are observed in a wide energy range above as well as below the ionization limits. These observations are in agreement with theoretical predictions based on a two-step model where the initially excited electron has no influence on the emission step. Excess population of states in the conduction band is found, and discussed in terms of attosecond electron dynamics. 14. Nonlinear Zeeman effect in photoassociation spectra of ^{40}Ca near the ^3P_1+^1S_0 asymptote CERN Document Server Tiemann, Eberhard; Pachomow, Evgenij; Riehle, Fritz; Sterr, Uwe 2015-01-01 We present calculations of the Zeeman effect of narrow photoassociation lines of ^{40}Ca near the ^3P_1 + ^1S_0 asymptote. Using a coupled-channel model we find a nonlinear Zeeman effect that even at low fields of a few mT amounts to several kHz. With this model we analyze previous measurements and give corrected long range dispersion coefficients of the ^3\\Pi_{u} and ^3\\Sigma^+ _{u} states. 15. A software platform to analyse the ethical issues of electronic patient privacy policy: the S3P example OpenAIRE Mizani, M A; Baykal, N 2007-01-01 Paper‐based privacy policies fail to resolve the new changes posed by electronic healthcare. Protecting patient privacy through electronic systems has become a serious concern and is the subject of several recent studies. The shift towards an electronic privacy policy introduces new ethical challenges that cannot be solved merely by technical measures. Structured Patient Privacy Policy (S3P) is a software tool assuming an automated electronic privacy policy in an electronic healthcare setting... 16. Cu6S4 cluster based twelve-connected face-centered cubic and Cu19I4S12 cluster based fourteen-connected body-centered cubic topological coordination polymers. Science.gov (United States) Hao, Zheng-Ming; Fang, Rui-Qin; Wu, Hai-Shun; Zhang, Xian-Ming 2008-09-15 Hydrothermal reaction of Cu(MeCO2)2, (4-pyridylthio)acetic acid and NH4SCN resulted in a twelve-connected face-centered cubic topological metal-organic framework [Cu3(pdt)2(CN)] (pdt = pyridinethiolate) in which Cu6S4 clusters act as twelve-connected nodes and pyridine rings and cyanides act as connectors. As an extension, an unprecedented fourteen-connected body-centered cubic coordination polymer [Cu19I4(pdt)12(SH)3] has been synthesized by three methods, in which nanosized chiral Cu19I4S12 clusters act as fourteen-connected nodes and triple pyridine rings and hydrosulfides act as connectors. The in situ S-C(sp(3)), S-C(sp(2)), and S-C(sp) cleavage reactions have been observed in the work. 17. miR-130b-3p Upregulation Contributes to the Development of Thyroid Adenomas Targeting CCDC6 Gene Science.gov (United States) Leone, Vincenza; Langella, Concetta; Esposito, Francesco; De Martino, Marco; Decaussin-Petrucci, Myriam; Chiappetta, Gennaro; Bianco, Antonio; Fusco, Alfredo 2015-01-01 We have previously studied the function of microRNAs (miRNAs) in thyroid cells using the differentiated rat thyroid PC Cl 3 cells that need thyrotropin (TSH) for their growth. The miRNA expression profile examination allowed the detection of a set of miRNAs downregulated and upregulated by TSH. Here, we first demonstrated that upregulation of miR-130b-3p occurs through a protein kinase A-cAMP-responsive element binding protein (CREB)-dependent mechanism. Then, we analyzed its expression in human thyroid follicular adenomas, where a constitutive CREB activation is frequently present. miR-130b-3p results in upregulation with a high fold-change in most thyroid follicular adenomas. Then, we identified CCDC6, coding for a protein that interacts with CREB1 leading to the transcriptional repression of CREB1 target genes, as a target of this miRNA. The targeting of CCDC6 by miR-130b-3p likely accounts for the mechanism by which its upregulation contributes to the development of thyroid adenomas increasing CREB1 activity. PMID:26835423 18. New high-pressure polymorph of In2S3 with defect Th3P4-type structure Science.gov (United States) Lai, Xiaojing; Zhu, Feng; Wu, Ye; Huang, Rong; Wu, Xiang; Zhang, Qian; Yang, Ke; Qin, Shan 2014-02-01 The high pressure behavior of β-In2S3 (I41/amd and Z=16) has been studied by in situ synchrotron radiation X-ray diffraction combined with diamond anvil cell up to 71.7 GPa. Three pressure-induced phase transitions are evidenced at ~6.6 GPa, ~11.1 GPa at room temperature and 35.6 GPa after the high-temperature annealing using a portable laser heating system. The new polymorph of In2S3 at 35.6 GPa is assigned to the denser cubic defect Th3P4 structure (I4bar3d and Z=5.333), whose unit-cell parameters are a=7.557(1) Å and V=431.6(2) Å3. The Th3P4-type phase can be stable at least up to 71.7 GPa and cannot be preserved at ambient pressure. The pressure-volume relationship is well described by the second-order Birch-Murnaghan Equation of State, which yields B0=63(3) GPa and B0‧=4 (fixed) for the β-In2S3 phase and B0=87(3) GPa and B0‧=4 (fixed) for the defect Th3P4-type phase respectively. 19. Delineation of the 3p14.1p13 microdeletion associated with syndromic distal limb contractures. Science.gov (United States) Thevenon, Julien; Monnier, Nicole; Callier, Patrick; Dieterich, Klaus; Francoise, Michel; Montgomery, Tara; Kjaergaard, Susanne; Neas, Katherine; Dixon, Joanne; Dahm, Thomas Lee; Huet, Frédéric; Ragon, Clémence; Mosca-Boidron, Anne-Laure; Marle, Nathalie; Duplomb, Laurence; Aubriot-Lorton, Marie-Hélène; Mugneret, Francine; Vokes, Steve A; Tucker, Haley W; Lunardi, Joël; Faivre, Laurence; Jouk, Pierre Simon; Thauvin-Robinet, Christel 2014-12-01 Distal limb contractures (DLC) represent a heterogeneous clinical and genetic condition. Overall, 20-25% of the DLC are caused by mutations in genes encoding the muscle contractile apparatus. Large interstitial deletions of the 3p have already been diagnosed by standard chromosomal analysis, but not associated with a specific phenotype. We report on four patients with syndromic DLC presenting with a de novo 3p14.1p13 microdeletion. The clinical features associated multiple contractures, feeding problems, developmental delay, and intellectual disability. Facial dysmorphism was constant with low-set posteriorly rotated ears and blepharophimosis. Review of previously reported cases with a precise mapping of the deletions, documented a 250 kb smallest region of overlap (SRO) necessary for DLC. This region contained one gene, EIF4E3, the first three exons of the FOXP1 gene, and an intronic enhancer of FOXP1 named hs1149. Sanger sequencing and locus quantification of hs1149, EIF4E3, and FOXP1 in a cohort of 11 French patients affected by DLC appeared normal. In conclusion, we delineate a new microdeletion syndrome involving the 3p14.1p13 locus and associated with DLC and severe developmental delay. © 2014 Wiley Periodicals, Inc. 20. A new form of Ca{sub 3}P{sub 2} with a ring of Dirac nodes Energy Technology Data Exchange (ETDEWEB) Xie, Lilia S.; Schoop, Leslie M.; Seibel, Elizabeth M.; Gibson, Quinn D.; Xie, Weiwei; Cava, Robert J., E-mail: rcava@princeton.edu [Department of Chemistry, Princeton University, Princeton, New Jersey 08544 (United States) 2015-08-01 We report the synthesis and crystal structure of a new high-temperature form of Ca{sub 3}P{sub 2}. The crystal structure was determined through Rietveld refinements of synchrotron powder x-ray diffraction data. This form of Ca{sub 3}P{sub 2} has a crystal structure of the hexagonal Mn{sub 5}Si{sub 3} type, with a Ca ion deficiency compared to the ideal 5:3 stoichiometry. This yields a stable, charge-balanced compound of Ca{sup 2+} and P{sup 3−}. We also report the observation of a secondary hydride phase, Ca{sub 5}P{sub 3}H, which again is a charge-balanced compound. The calculated band structure of Ca{sub 3}P{sub 2} indicates that it is a three-dimensional Dirac semimetal with a highly unusual ring of Dirac nodes at the Fermi level. The Dirac states are protected against gap opening by a mirror plane in a manner analogous to what is seen for graphene. 1. Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model. Directory of Open Access Journals (Sweden) Abraham Lin Full Text Available Conjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. Strategies for inhibiting conjugation may be useful for preserving the effectiveness of antibiotics and preventing the emergence of bacterial strains with multiple resistances. Filamentous bacteriophages were first observed to inhibit conjugation several decades ago. Here we investigate the mechanism of inhibition and find that the primary effect on conjugation is occlusion of the conjugative pilus by phage particles. This interaction is mediated primarily by phage coat protein g3p, and exogenous addition of the soluble fragment of g3p inhibited conjugation at low nanomolar concentrations. Our data are quantitatively consistent with a simple model in which association between the pili and phage particles or g3p prevents transmission of an F plasmid encoding tetracycline resistance. We also observe a decrease in the donor ability of infected cells, which is quantitatively consistent with a reduction in pili elaboration. Since many antibiotic-resistance factors confer susceptibility to phage infection through expression of conjugative pili (the receptor for filamentous phage, these results suggest that phage may be a source of soluble proteins that slow the spread of antibiotic resistance genes. 2. Phase transformation and disorder effect on optical and electrical properties of Zn3P2 thin films. Science.gov (United States) El Zawawi, I K; Abdel Moez, A; Hammad, T R; Ibrahim, R S 2012-08-01 The phase transformation of zinc phosphide (Zn(3)P(2)) thin films was detected through isochronal annealing process. The effects on isochronal annealing on the internal structural, optical and electrical properties of deposited Zn(3)P(2) thin films have been discussed. The films were prepared by thermal evaporation under constant preparation conditions of vacuum 1.3×10(-5)Torr, substrate temperature (300K), rate of deposition (∼1nm/s) and film thickness (480nm). The annealing process was carried out under vacuum for 2h at different temperatures ranging from 373 to 623K. X-ray diffraction patterns showed that the as-deposited films and those annealed at temperatures less than 623K exhibit amorphous structure, while the films annealed at 623K showed tetragonal polycrystalline structure. The optical transmission and reflection spectra were measured at the wavelength range of 190-2500nm. The absorption coefficient spectra and the degree of disorder as measured from the absorption edge were determined. The indirect and direct optical energy band gaps were evaluated for indirect allowed and direct allowed transitions for amorphous and polycrystalline films, respectively. The refractive index n(o) increases with raising the annealing temperature which refers to more condensation in the material. The electrical resistivity for Zn(3)P(2) films decreases exponentially with raising the annealing temperature up to 623K as influenced by structure transformation and decreasing the degree of disorder in the films. 3. The fast C(3P) + CH3OH reaction as an efficient loss process for gas-phase interstellar methanol CERN Document Server Shannon, Robin J; Loison, Jean-Christophe; Caubet, Philippe; Balucani, Nadia; Seakins, Paul W; Wakelam, Valentine; Hickson, Kevin M 2014-01-01 Rate constants for the C(3P) + CH3OH reaction have been measured in a continuous supersonic flow reactor over the range 50 K to 296 K. C(3P) was created by the in-situ pulsed laser photolysis of CBr4, a multiphoton process which also produced some C(1D), allowing us to investigate simultaneously the low temperature kinetics of the C(1D) + CH3OH reaction. C(1D) atoms were followed by an indirect chemiluminescent tracer method in the presence of excess CH3OH. C(3P) atoms were detected by the same chemiluminescence technique and also by direct vacuum ultra-violet laser induced fluorescence (VUV LIF). Secondary measurements of product H(2S) atom formation have been undertaken allowing absolute H atom yields to be obtained by comparison with a suitable reference reaction. In parallel, statistical calculations have been performed based on ab-initio calculations of the complexes, adducts and transition states (TSs) relevant to the title reaction. By comparison with the experimental H atom yields, the preferred react... 4. MicroRNA 874-3p Exerts Skeletal Anabolic Effects Epigenetically during Weaning by Suppressing Hdac1 Expression* Science.gov (United States) Kushwaha, Priyanka; Khedgikar, Vikram; Sharma, Deepika; Yuen, Tony; Gautam, Jyoti; Ahmad, Naseer; Karvande, Anirudha; Mishra, Prabhat R.; Trivedi, Prabodh K.; Sun, Li; Bhadada, Sanjay K.; Zaidi, Mone; Trivedi, Ritu 2016-01-01 Embryonic skeletogenesis and postnatal bone development require the transfer of calcium from the mother to the offspring during pregnancy and lactation. Therefore, bone resorption in the mother becomes elevated during these periods, resulting in significant maternal skeletal loss. There follows an anabolic phase around weaning during which there is a remarkable recovery of the maternal skeleton. However, the mechanism(s) of this anabolic response remain(s) largely unknown. We identified eight differentially expressed miRNAs by array profiling, of which miR-874-3p was highly expressed at weaning, a time when bone loss was noted to recover. We report that this weaning-associated miRNA is an anabolic target. Therefore, an agomir of miR-874-3p induced osteoblast differentiation and mineralization. These actions were mediated through the inhibition of Hdac1 expression and enhanced Runx2 transcriptional activation. When injected in vivo, the agomir significantly increased osteoblastogenesis and mineralization, reversed bone loss caused by ovariectomy, and increased bone strength. We speculate that elevated miR-874-3p expression during weaning enhances bone formation and that this miRNA may become a therapeutic target for conditions of bone loss. PMID:26663087 5. Crednerite-CuMnO{sub 2} thin films prepared using atmospheric pressure plasma annealing Energy Technology Data Exchange (ETDEWEB) Chen, Hong-Ying, E-mail: hychen@cc.kuas.edu.tw [Department of Chemical and Materials Engineering, National Kaohsiung University of Applied Sciences, 415 Chiken Kuang Road, Kaohsiung 807, Taiwan, ROC (China); Lin, Yu-Chang [Department of Chemical and Materials Engineering, National Kaohsiung University of Applied Sciences, 415 Chiken Kuang Road, Kaohsiung 807, Taiwan, ROC (China); Lee, Jiann-Shing [Department of Applied Physics, National Pingtung University, 4-18 Minsheng Road, Pingtung City 900, Taiwan, ROC (China) 2015-05-30 Highlights: • Crednerite-CuMnO{sub 2} thin films were formed at atmospheric pressure plasma with N{sub 2}–(5–10)%O{sub 2}. • The binding energy of Cu-2p spectrum of the crednerite-CuMnO{sub 2} thin films was 932.3 eV (Cu{sup +}). • The binding energies of Mn-3p spectrum were 48.1 ± 0.2 eV (Mn{sup 3+}) and 50.0 ± 0.2 eV (Mn{sup 4+}). • The cation distribution in the crednerite-CuMnO{sub 2} thin films was Cu{sub 1.0}{sup +}(Mn{sub 0.6}{sup 3+}Mn{sub 0.4}{sup 4+})O{sub 2}. • The electrical conductivity of CuMnO{sub 2} thin films was (2.61–2.65) × 10{sup 4} Ω cm. - Abstract: This study reports the preparation of crednerite-CuMnO{sub 2} thin films using atmospheric pressure plasma annealing. The pristine thin films were deposited onto a quartz substrate using the sol–gel process. The specimens were then annealed using atmospheric pressure plasma at N{sub 2}–(0–20%)O{sub 2} for 20 min. Crednerite-CuMnO{sub 2} thin films were obtained using atmospheric pressure plasma annealing at N{sub 2}–5%O{sub 2} and N{sub 2}–10%O{sub 2}. The lattice parameters of the thin films were a = 0.5574–0.5580 nm, b = 0.2874–0.2879 nm, c = 0.5878–0.5881 nm, and β = 104.15–104.25°, which agree well with previous reports. The Raman shifts of the crednerite-CuMnO{sub 2} thin films were 688 ± 2 cm{sup −1}, 381 ± 2 cm{sup −1}, and 314 ± 2 cm{sup −1}. The binding energy of Cu-2p spectrum of the crednerite-CuMnO{sub 2} thin films was 932.3 ± 0.2 eV representing the Cu{sup +} in the thin films. The binding energies of Mn-3p spectrum were 48.1 ± 0.2 eV (Mn{sup 3+}) and 50.0 ± 0.2 eV (Mn{sup 4+}). Furthermore, the cation distribution in the thin films was Cu{sup +}{sub 1.0}(Mn{sup 3+}{sub 0.6}Mn{sup 4+}{sub 0.4})O{sub 2} from the X-ray photoelectron spectroscopy measurement. When the crednerite-CuMnO{sub 2} phase was formed, the surface morphology exhibited a compact/dense granular morphology. The optical bandgap of the crednerite-CuMnO{sub 2} thin 6. 含B2-CuZr相CuZr基块体金属玻璃复合材料应变速率敏感性的变化%Strain Rate Sensitivity Variation in CuZr-based Bulk Metallic Glass Composites Containing B2-CuZr Phase Institute of Scientific and Technical Information of China (English) 魏然; 昌云; 杨森; 张长军; 贺林 2016-01-01 通过应变速率跳跃模式下的单轴压缩试验,研究了含有不同体积分数B2-CuZr相的CuZr基块体金属玻璃复合材料的应变速率敏感性.结果发现,随着B2-CuZr相体积分数的增加,在3.7×10-5s-1至3.7×10-3 s-1的应变速率范围中,该复合材料的应变速率敏感指数可由负值变化为正值.但是,对于B2-CuZr相体积分数高达约80％的复合材料,其应变速率敏感指数的正值仍然是反常的低.这一现象与B2-CuZr相自身力学行为所呈现的有限应变速率依赖性有关,是由于变形过程中B2-CuZr相的马氏体相变效应所致.研究结果显示,CuZr基块体金属玻璃复合材料的极限应变速率敏感性受制于B2-CuZr相.%The strain rate sensitivity (SRS) of CuZr-based bulk metallic glass composites (BMGCs) containing a B2-CuZr phase with different volume fractions was investigated by performing uniaxial compression tests in a strain-rate-jump mode.It is found that the SRS exponents of the BMGCs can vary from negative to positive in the strain-rate range from 3.7× 10-5 s-1 to 3.7× 103 s-1 with increasing of the B2-CuZr phase volume fraction.However,the positive SRS exponent of the BMGC with a higher B2-CuZr phase volume fraction up to about 80％ is still unexpectedly low.This is attributed to the limited strain-rate-dependence of mechanical behavior of the B2-CuZr phase,which is induced by the martensitic transformation effect of the phase during deformation.It is obvious that the SRS ceiling of the CuZr-based BMGCs is controlled by the B2-CuZr phase. 7. Ionisation from the 3s sub-level of highly charged ions Science.gov (United States) Golden, L. B.; Sampson, D. H.; Omidvar, K. 1978-01-01 Scaled electron-impact cross sections are calculated for ionization from the 3s sublevel of hydrogenic ions with Z equal infinity by use of the Born exchange or the Coulomb-Born Oppenheimer approximation (which is exact, apart from relativistic corrections, in this limit). The results are fitted to an analytic expression which goes into the correct Bethe approximation result at high energies and which can readily be integrated over a Maxwellian electron velocity distribution to obtain collision rates. These results permit calculation of the approximate cross section and collision rate for ionization from the 3s sublevel of any highly charged ion with Z/N larger than approximately 2. Results obtained by the described procedure for Fe-14(+) and Fe-15(+) are compared with results obtained by other procedures. 8. Evidence for a heated gas bubble inside the "cooling flow" region of MKW3s CERN Document Server Mazzotta, P; Paerels, F B S; Ferrigno, C; Colafrancesco, Sergio; Mewe, R; Forman, W R 2002-01-01 We report on the deep Chandra observation of central r=200kpc region of the cluster of galaxies MKW3s which was previously identified as a moderate cooling flow cluster. The Chandra image reveals two striking features -- a 100kpc long and 21kpc wide filament, extending from the center to the south-west and a nearly circular, 50kpc diameter depression 90 kpc south of the X-ray peak. The temperature map shows that the filamentary structure is colder while the surface brightness depression is hotter than the average cluster temperature at any radius. The hot and the cold regions indicate that both cooling and heating processes are taking place in the center of MKW3s. We argue that the surface brightness depression is produced by a heated, low-density gas bubble along the line of sight. We suggest that the heated bubble is produced by short-lived nuclear outbursts from the central galaxy. 9. Internationally Recommended Best Practices in Transportation Financing Public-Private Partnerships (P3s) DEFF Research Database (Denmark) Martin, Lawrence; Lawther, Wendell; Hodge, Graeme 2013-01-01 Transportation financing public-private partnerships (P3s) are a common practice in many countries. However, they represent a relatively new approach to transportation infrastructure financing for state and local governments in the United States. In a transportation financing P3 project, a private...... sector partner designs-builds-finances-operates- maintains (DBFOM) a transportation infrastructure asset (road, highway, bridge, tunnel, etc.) with an emphasis on financing. Under this type of arrangement, the private sector partner is primarily responsible for securing all or substantially all...... of the funding necessary to construct new transportation infrastructure and/or rehabilitate existing transportation infrastructure. This study reviews the international experience of national and sub-national governments with transportation financing P3s. The primary purpose of this study is to identify... 10. Synthetic Ni3S2/Ni hybrid architectures as potential contrast agents in MRI Science.gov (United States) Ma, J.; Chen, K. 2016-04-01 Traditional magnetic resonance imaging (MRI) contrast agents mainly include superparamagnetic (SPM) iron oxide nanoparticle as T 2 contrast agent for liver and paramagnetic Gd (III)-chelate as T 1 contrast agent for all organs. In this work, weak ferromagnetic kale-like and SPM cabbage-like Ni3S2@Ni hybrid architectures were synthesized and evaluated as potential T 1 MRI contrast agents. Their relatively small r 2/r 1 ratios of 2.59 and 2.38, and high r 1 values of 11.27 and 4.89 mmol-1 L s-1 (for the kale-like and cabbage-like Ni3S2@Ni, respectively) will shed some light on the development of new-type MRI contrast agents. 11. Mo3S4 Clusters as an Effective H2 Evolution Catalyst on Protected Si Photocathodes DEFF Research Database (Denmark) Seger, Brian; Herbst, Konrad; Pedersen, Thomas 2014-01-01 This work shows how a molecular Mo3S4 cluster bonded to a photoelectrode surface via a phosphonate ligand can be a highly effective co-catalyst in photocathodic hydrogen evolution systems. Using a TiO2 protected n+p Si photocathode, H2 evolution occurs with an onset of +0.33 V vs. RHE in an acid...... solution with this precious metal-free system. Using just the red part of the AM1.5 solar spectrum (λ > 635 nm), a saturation current of 20 mA/cm2 is achieved from an electrode containing Mo3S4 dropcasted onto a 100 nm TiO2/7 nm Ti/n+p Si electrode.... 12. The impact of climatic on 3S tourism in Samsun, Turkey: The summer 2008 experience Directory of Open Access Journals (Sweden) Kemalettin Şahin 2010-03-01 Full Text Available The objective of this study is to put forth the relationship between 3S (sand-sea-sun tourism in Samsun and climate conditions. That is why observations were made during the summer of 2008 (July, august at the beaches selected by sampling method. The meteorological data about the time of day during which observations were made were taken from the internet site of the general directorate of meteorology. Also biometeorological thermal comfort indexes of Samsun were created. According to the data obtained, the months of July and august form the period during which tourism and recreational activities are at the highest level in the beaches of Samsun. During the sea season (July and august the total number of days during which the wind speed was greater than or equal to 21.6 km/h limiting the usage of the beach and the sea was 17; the total number of days during which the sky was “mostly cloudy” was 20 (July 10 days, august 10 days. When all the other variables are considered (rain, condition of the sea... the foremost ones being wind and cloud cover, it was determined that the total duration of 3S tourism in the area studied was intermittently 35 ±5 days. According to the thermal comfort index 94% of July and august are composed of “hot” days. According to humidity index 29 % of July and 13 % of august are composed of mild-calm days. As a result the most important natural factors negatively affecting 3S tourism in Samsun are Samsun’s general location, its position in the general atmospheric circulation system and as a result of these the climate conditions and daily weather conditions. In this sense, it will be suitable that in order for Samsun to obtain a “tourism city” identity, it should be evaluated with the other alternative tourism appeals of 3S tourism. 13. The MAP3S network data and quality control summary for 1985 Energy Technology Data Exchange (ETDEWEB) Dana, M.T. 1988-02-01 This report, the ninth in a series documenting results from the MAP3S Precipitation Chemistry Network, contains a statistical summary of daily precipitation chemistry data from the nine-site network in the eastern United States, both for the year 1985 alone and for the period 1977 through 1985. In addition, a report on quality control and external quality assurance activities is included, which updates the 1980 quality control report. 14. The MAP3S Precipitation Chemistry Network: Data and quality control summary for 1986 and 1987 Energy Technology Data Exchange (ETDEWEB) Dana, M.T.; Barchet, W.R. 1989-05-01 This report, the tenth in a series documenting results from the MAP3S Precipitation Chemistry Network, contains a statistical summary of daily precipitation chemistry data from the nine-site network in the eastern United States, both for the years 1986 and 1987 individually and for the period 1977 through 1987. In addition, external quality assurance results for 1986 and 1987 are summarized. 17 refs., 21 figs., 20 tabs. 15. Power management implementation in FreeRTOS on LM3S3748 Directory of Open Access Journals (Sweden) Simonović Mirela 2013-01-01 Full Text Available Power consumption has become a major concern of embedded systems today. With the aim to reduce power consumption during the runtime, operating systems are dealing with power management. In this work, the FreeRTOS port is extended with power management features on LM3S3748 microcontroller. Tickless idle technique is implemented to provide more powersaving during the processor idle periods. 16. BOREAS Level-3s SPOT Imagery: Scaled At-sensor Radiance in LGSOWG Format Science.gov (United States) Strub, Richard; Nickeson, Jaime; Newcomer, Jeffrey A.; Hall, Forrest G. (Editor); Cihlar, Josef 2000-01-01 For BOReal Ecosystem-Atmosphere Study (BOREAS), the level-3s Satellite Pour l'Observation de la Terre (SPOT) data, along with the other remotely sensed images, were collected in order to provide spatially extensive information over the primary study areas. This information includes radiant energy, detailed land cover, and biophysical parameter maps such as Fraction of Photosynthetically Active Radiation (FPAR) and Leaf Area Index (LAI). The SPOT images acquired for the BOREAS project were selected primarily to fill temporal gaps in the Landsat Thematic Mapper (TM) image data collection. CCRS collected and supplied the level-3s images to BOREAS Information System (BORIS) for use in the remote sensing research activities. Spatially, the level-3s images cover 60- by 60-km portions of the BOREAS Northern Study Area (NSA) and Southern Study Area (SSA). Temporally, the images cover the period of 17-Apr-1994 to 30-Aug-1996. The images are available in binary image format files. Due to copyright issues, the SPOT images may not be publicly available. 17. Competition of superconductivity with the structural transition in M o3S b7 Science.gov (United States) Ye, G. Z.; Cheng, J.-G.; Yan, J.-Q.; Sun, J. P.; Matsubayashi, K.; Yamauchi, T.; Okada, T.; Zhou, Q.; Parker, D. S.; Sales, B. C.; Uwatoko, Y. 2016-12-01 Prior to the superconducting transition at Tc≈2.3 K , M o3S b7 undergoes a symmetry-lowering, cubic-to-tetragonal structural transition at Ts=53 K . We have monitored the pressure dependence of these two transitions by measuring the resistivity of M o3S b7 single crystals under various hydrostatic pressures up to 15 GPa. The application of external pressure enhances Tc but suppresses Ts until Pc≈10 GPa , above which a pressure-induced first-order structural transition takes place and is manifested by the phase coexistence in the pressure range 8 ≤P ≤12 GPa . The cubic phase above 12 GPa is also found to be superconducting with a higher Tc≈6 K that decreases slightly with further increasing pressure. The variations with pressure of Tc and Ts satisfy the Bilbro-McMillan equation, i.e. Tc nTs 1 -n= constant, thus suggesting the competition of superconductivity with the structural transition that has been proposed to be accompanied with a spin-gap formation at Ts. Our first-principles calculations suggest the importance of magnetism that competes with the superconductivity in M o3S b7 . 18. Bimane fluorescence scanning suggests secondary structure near the S3-S4 linker of BK channels. Science.gov (United States) Semenova, Nina P; Abarca-Heidemann, Karin; Loranc, Eva; Rothberg, Brad S 2009-04-17 Gating of large conductance Ca(2+)-activated K(+) channels (BK or maxi-K channels) is controlled by a Ca(2+)-sensor, formed by the channel cytoplasmic C-terminal domain, and a voltage sensor, formed by its S0-S4 transmembrane helices. Here we analyze structural properties of a portion of the BK channel voltage sensing domain, the S3-S4 linker, using fluorescence lifetime spectroscopy. Single residues in the S3-S4 linker region were substituted with cysteine, and the cysteine-substituted mutants were expressed in CHO cells and covalently labeled with the sulfhydryl-reactive fluorophore monobromo-trimethylammonio-bimane (qBBr). qBBr fluorescence is quenched by tryptophan and, to a lesser extent, tyrosine side chains. We found that qBBr fluorescence in several of the labeled cysteine-substituted channels shows position-specific quenching, as indicated by increase of the brief lifetime component of the qBBr fluorescence decay. Quenching was reduced with the mutation W203F (in the S4 segment), suggesting that Trp-203 acts as a quenching group. Our results suggest a working hypothesis for the secondary structure of the BK channel S3-S4 region, and places residues Leu-204, Gly-205, and Leu-206 within the extracellular end of the S4 helix. 19. First-principles study of low Miller index Ni3S2 surfaces in hydrotreating conditions. Science.gov (United States) Aray, Yosslen; Vega, David; Rodriguez, Jesus; Vidal, Alba B; Grillo, Maria Elena; Coll, Santiago 2009-03-12 Density functional theory (DFT) calculations combined with surface thermodynamic arguments and the Gibbs-Curie-Wulff equilibrium morphology formalism have been employed to explore the effect of the reaction conditions, temperature (T), and gas-phase partial pressures (PH2 and PH2S) on the stability of nickel sulfide (Ni3S2) surfaces. Furthermore, the strength and nature of chemical bonds for selected Ni3S2 surface cuts were investigated with the quantum theory of atoms in molecules methodology. A particular analysis of the electrostatic potential within this theoretical framework is performed to study the potential activity of nickel sulfide nanoparticles as hydrodesulfurization (HDS) catalysts. The calculated thermodynamic surface stabilities and the resulting equilibrium morphology model suggest that unsupported Ni3S2 nanoparticles mainly expose (111) and (111) type surface faces in HDS conditions. Analysis of the electrostatic potential mapped onto a selected electron density isocontour (0.001 au) on those expose surface reveals a poor potential reactivity toward electron-donating reagents (i.e., low Lewis acidity). Consequently, a very low attraction between coordinatively unsaturated active sites (Lewis sites) exposed at the catalytic particles and the S atoms coming from reagent polluting molecules does inactive these kinds of particles for HDS. 20. Discussion on the application of 3S technology in soil and water conservation project Science.gov (United States) Qin, Fen; Hu, Sujie; Wang, Yanan; Tang, Fuping 2007-06-01 Soil and water conservation project in the loess plateau of China is focus on improving its feasibility with new technology. This paper discussed on the application of "3S" technology in a soil and water conservation project of selected small watershed of Jingjiahe, Loess plateau. The research presents an approach to apply RUSLE to quantify soil and water loss by the help of "3S" technology, the suitability evaluation for each piece of land is implemented with the assessment indices. Finally, according to the nature condition and economic development trend, by accomplishing the analysis of the comprehensive control benefits for the small watershed, establishing rational land use pattern. Through five years construction of soil and water conservation, the controlling soil erosion area up to 2369.98 hectares, harnessed degree reaches 80.66%, the per capita net income will float up from yen 944.72 to yen 1049.75, the annual preserved area of endangered soil will be 90,000t with the efficiency increased by more than 22%, eventually the soil and water loss shall be well under control. This indicates that "3S" technology make the work more cost-effective, more efficient and more reliable taking into account the precise data which are processed in the planning. 1. miR-142-3p as a biomarker of blastocyst implantation failure - A pilot study Science.gov (United States) Borges Jr., Edson; Setti, Amanda Souza; Braga, Daniela P.A.F.; Geraldo, Murilo V; Figueira, Rita de Cássia S; Iaconelli Jr, Assumpto 2016-01-01 Objective This study aims to find whether microRNAs (miRNAs) detected in the culture medium of embryos produced in vitro could be potential biomarkers of embryo implantation. Methods Culture media samples from 36 embryos, derived from patients undergoing intracytoplasmic sperm injection (ICSI) in a private university-affiliated IVF center, were collected between January/2015 and November/2015. Samples were collected on day three and embryo transfers were performed on day five and all embryos reached the blastocyst stage. Samples were split into groups according to the embryo implantation result: Positive-Implantation-Group (n=18) or Negative-Implantation-Group (n=18). For the first analysis, samples were pooled in three sets for each group (6-7 spent media per pool). MicroRNAs were extracted from spent media and cDNA was synthesized. C. elegans miR-39 was used as RNA spike-in to normalize the gene expression analysis. The expression of microRNAs into the spent media from the Positive-Implantation-Group was compared with those from the Negative-Implantation-Group. A set of seven miRNAs (miR-21, miR-142-3p, miR-19b, miR-92a, miR-20b, miR-125a and miR148a) selected according with the literature, was tested. To check whether miRNAs could be detected in individual samples of culture media, in a second analysis, ten more samples were tested for miR-21 and miR-142-3p. Results From the sevens tested miRNAs, a significant increased expression of miR-142-3p could be noted in the Negative-Implantation-Group (P<0.001). For other three miRNAs (miR-21, miR-19b and miR-92a) a difference in expression was observed, however it did not reach a statistical significance. In addition, when ten non-redundant samples were tested to check if miRNAs could be detected in individual samples of culture media, the highly specific amplification of mature miRNAs, including miR-142-3p, could be noted. Conclusion Our findings suggest that miR-142-3p, previously described as a tumor suppressor and 2. Yeast mitochondrial biogenesis: a role for the PUF RNA-binding protein Puf3p in mRNA localization. Directory of Open Access Journals (Sweden) Yann Saint-Georges Full Text Available The asymmetric localization of mRNA plays an important role in coordinating posttranscriptional events in eukaryotic cells. We investigated the peripheral mitochondrial localization of nuclear-encoded mRNAs (MLR in various conditions in which the mRNA binding protein context and the translation efficiency were altered. We identified Puf3p, a Pumilio family RNA-binding protein, as the first trans-acting factor controlling the MLR phenomenon. This allowed the characterization of two classes of genes whose mRNAs are translated to the vicinity of mitochondria. Class I mRNAs (256 genes have a Puf3p binding motif in their 3'UTR region and many of them have their MLR properties deeply affected by PUF3 deletion. Conversely, mutations in the Puf3p binding motif alter the mitochondrial localization of BCS1 mRNA. Class II mRNAs (224 genes have no Puf3p binding site and their asymmetric localization is not affected by the absence of PUF3. In agreement with a co-translational import process, we observed that the presence of puromycin loosens the interactions between most of the MLR-mRNAs and mitochondria. Unexpectedly, cycloheximide, supposed to solidify translational complexes, turned out to destabilize a class of mRNA-mitochondria interactions. Classes I and II mRNAs, which are therefore transported to the mitochondria through different pathways, correlated with different functional modules. Indeed, Class I genes code principally for the assembly factors of respiratory chain complexes and the mitochondrial translation machinery (ribosomes and translation regulators. Class II genes encode proteins of the respiratory chain or proteins involved in metabolic pathways. Thus, MLR, which is intimately linked to translation control, and the activity of mRNA-binding proteins like Puf3p, may provide the conditions for a fine spatiotemporal control of mitochondrial protein import and mitochondrial protein complex assembly. This work therefore provides new openings 3. Ambient atmosphere-processable, printable Cu electrodes for flexible device applications: structural welding on a millisecond timescale of surface oxide-free Cu nanoparticles Science.gov (United States) Oh, Sang-Jin; Jo, Yejin; Lee, Eun Jung; Lee, Sun Sook; Kang, Young Hun; Jeon, Hye-Ji; Cho, Song Yun; Park, Jin-Seong; Seo, Yeong-Hui; Ryu, Beyong-Hwan; Choi, Youngmin; Jeong, Sunho 2015-02-01 Recently, various functional devices based on printing technologies have been of paramount interest, owing to their characteristic processing advantages along with excellent device performance. In particular, printable metallic electrodes have drawn attention in a variety of optoelectronic applications; however, research into printable metallic nanoparticles has been limited mainly to the case of an environmentally stable Ag phase. Despite its earth-abundance and highly conductive nature, the Cu phase, to date, has not been exploited as an ambient atmosphere-processable, printable material due to its critical oxidation problem in air. In this study, we demonstrate a facile route for generating highly conductive, flexible Cu electrodes in air by introducing the well-optimized photonic sintering at a time frame of 10-3 s, at which the photon energy, rather than conventional thermal energy, is instantly provided. It is elucidated here how the surface oxide-free, printed Cu particulate films undergo chemical structural/microstructural evolution depending on the instantly irradiated photon energy, and a successful demonstration is provided of large-area, flexible, printed Cu conductors on various substrates, including polyimide (PI), polyethersulfone (PES), polyethylene terephthalate (PET), and paper. The applicability of the resulting printed Cu electrodes is evaluated via implementation into both flexible capacitor devices and indium-gallium-zinc oxide (IGZO) flexible thin-film transistors.Recently, various functional devices based on printing technologies have been of paramount interest, owing to their characteristic processing advantages along with excellent device performance. In particular, printable metallic electrodes have drawn attention in a variety of optoelectronic applications; however, research into printable metallic nanoparticles has been limited mainly to the case of an environmentally stable Ag phase. Despite its earth-abundance and highly conductive 4. GMI in FeCuNbSiB/Cu multilayers Energy Technology Data Exchange (ETDEWEB) Correa, M.A. [Departamento de Fisica, CCNE, UFSM, 97105-900, Santa Maria, RS (Brazil)]. E-mail: mmacorrea@gmail.com; Viegas, A.D.C. [Departamento de Fisica, CCNE, UFSM, 97105-900, Santa Maria, RS (Brazil); Silva, R.B. da [Departamento de Fisica, CCNE, UFSM, 97105-900, Santa Maria, RS (Brazil); Andrade, A.M.H. de [Departamento de Fisica, CCNE, UFSM, 97105-900, Santa Maria, RS (Brazil); Sommer, R.L. [Centro Brasileiro de Pesquisas Fisicas, 22290-180, Rio de Janeiro, RJ (Brazil) 2006-10-01 Very high magnetoimpedance (MI) measured at frequencies up to 1.8 GHz in single and multilayered thin films with composition Fe{sub 73.5}Cu{sub 1}Nb{sub 3}Si{sub 13.5}B{sub 9} and (Fe{sub 73.5}Cu{sub 1}Nb{sub 3}Si{sub 13.5}B{sub 9}+Cu) are reported. The magnetic properties of both systems are also compared. MI ratio as high as 300% for the multilayered samples were obtained. 5. MiR-100-3p and miR-877-3p regulate overproduction of IL-8 and IL-1β in mesangial cells activated by secretory IgA from IgA nephropathy patients. Science.gov (United States) Liang, Yan; Zhao, Guoqiang; Tang, Lin; Zhang, Junjun; Li, Tianfang; Liu, Zhangsuo 2016-10-01 IgA nephropathy (IgAN) is the most common type of primary glomerulonephritis, characterized by mesangial deposition of pathogenic IgA and the injury to mesangial cells. Our previous studies indicate that secretory IgA (SIgA) plays an important role in the pathogenesis of IgAN, and miR-16 is involved in destructive process in mesangial cells mediated by the SIgA from IgAN patients. Our current study aimed to study the role of miRNAs in the effect of SIgA from IgAN patients on mesangial cells. MicroRNA microarray and cytokines assay were performed to obtain the differential microRNAs expression profile in human renal mesangial cells stimulated by SIgA from IgAN patients (P-SIgA) with the cells treated by SIgA from healthy subjects (N-SgA) as control. The microRNAs with the most significant differences in microarray analysis were validated by quantitative RT-PCR. Among them, miR-100-3p and miR-877-3p were selected to predict target gene related to cytokines detecting in this study. Fifty-six differentially expressed microRNAs were chosen and 17 microRNAs with the most prominent changes were validated. Compared with N-SIgA, P-SIgA increased the production of interleukin (IL)-1β, IL-8, monocyte chemotactic protein-1 and transforming growth factor-β1. In addition, we for the first time demonstrated that over-production of IL-8 induced by the SIgA was regulated by down-expression of miR-100-3p in mesangial cells. Similarly, IL-1β over-production was regulated by down-expression of miR-877-3p. Our findings represent a pathogenic microRNAs expression profiling in human mesangial cells activated by P-SIgA. Furthermore, we provide a new explanation characterizing the molecular mechanism responsible for the regulation of IL-1β and IL-8 production in P-SIgA-triggered mesangial cells. Copyright © 2016 Elsevier Inc. All rights reserved. 6. Cu-Cr Literature Review Energy Technology Data Exchange (ETDEWEB) Need, Ryan F. [Los Alamos National Laboratory 2012-08-09 Cu-Cr alloys are part of a class of face-centered cubic (FCC)-body-centered cubic (BCC) composites that includes similar alloys, such as Cu-Nb and Cu-Ta. When heavily deformed, these FCC-BCC materials create 'in situ' composites with a characteristic structure-nanoscale BCC filaments in a ductile FCC matrix. The strength of these composites is vastly greater than predicted by the rule of mixtures, and has been shown to be inversely proportional to the filament spacing. Lower raw materials costs suggest that Cu-Cr alloys may offer more economical solution to high-strength, high-conductivity wire than either their Nb or Ta counterparts. However, Cr is also more brittle and soluble in Cu than Nb or Ta. These qualities necessitate thermal treatments to remove solute atoms from the Cu matrix, improve conductivity, and maintain the ductility of the Cr filaments. Through the use of different thermomechanical processing routes or the addition of select dopants, alloys with strength in excess of 1 GPa at 70% IACS have been achieved. To date, previous research on Cu-Cr alloys has focused on a relatively small number of alloy compositions and processing methods while the effects of dopants and ageing treatments have only been studied independently. Consequently, there remains considerable opportunity for the development and optimization of these alloys as a leading high-strength, high-conductivity material. 7. Atomic layer deposition of ultrathin Cu{sub 2}O and subsequent reduction to Cu studied by in situ x-ray photoelectron spectroscopy Energy Technology Data Exchange (ETDEWEB) Dhakal, Dileep [Center for Microtechnologies—ZfM, Technische Universität Chemnitz, D-09107 Chemnitz (Germany); Assim, Khaybar; Lang, Heinrich [Institute of Chemistry, Inorganic Chemistry, Technische Universität Chemnitz, D-09107 Chemnitz (Germany); Bruener, Philipp; Grehl, Thomas [ION-TOF GmbH, Heisenbergstr. 15, D-48149 Münster (Germany); Georgi, Colin; Waechtler, Thomas; Ecke, Ramona; Schulz, Stefan E., E-mail: stefan.schulz@zfm.tu-chemnitz.de; Gessner, Thomas [Center for Microtechnologies—ZfM, Technische Universität Chemnitz, D-09107 Chemnitz, Germany and Fraunhofer Institute for Electronic Nano Systems—ENAS, Technologie-Campus 3, D-09126 Chemnitz (Germany) 2016-01-15 The growth of ultrathin (<5 nm) Ru-doped Cu{sub 2}O films deposited on SiO{sub 2} by atomic layer deposition (ALD) and Cu films by subsequent reduction of the Cu{sub 2}O using HCO{sub 2}H or CO is reported. Ru-doped Cu{sub 2}O has been deposited by a mixture of 16: 99 mol. % of [({sup n}Bu{sub 3}P){sub 2}Cu(acac)] as Cu precursor and 17: 1 mol. % of [Ru(η{sup 5}-C{sub 7}H{sub 11})(η{sup 5}-C{sub 5}H{sub 4}SiMe{sub 3})] as Ru precursor. The catalytic amount of Ru precursor was to support low temperature reduction of Cu{sub 2}O to metallic Cu by formic acid (HCO{sub 2}H) on arbitrary substrate. In situ x-ray photoelectron spectroscopy investigations of the Cu{sub 2}O ALD film indicated nearly 1 at. % of carbon contamination and a phosphorous contamination below the detection limit after sputter cleaning. Systematic investigations of the reduction of Ru-doped Cu{sub 2}O to metallic Cu by HCO{sub 2}H or CO as reducing agents are described. Following the ALD of 3.0 nm Cu{sub 2}O, the ultrathin films are reduced between 100 and 160 °C. The use of HCO{sub 2}H at 110 °C enabled the reduction of around 90% Cu{sub 2}O. HCO{sub 2}H is found to be very effective in the removal of oxygen from Ru-doped Cu{sub 2}O films with 2.5–4.7 nm thickness. In contrast, CO was effective for the removal of oxygen from the Cu{sub 2}O films only below 3.0 nm at 145 °C. Root mean square surface roughness of 0.4 ± 0.1 nm was observed from atomic force microscopy (AFM) investigations after the ALD of Cu{sub 2}O, followed by the subsequent reduction of 3.0 nm Cu{sub 2}O using either HCO{sub 2}H at 110 °C or CO at 145 °C on SiO{sub 2}. Furthermore, ex situ low energy ion scattering and AFM investigations confirmed that the Cu{sub 2}O film after ALD and Cu films after subsequent reduction was continuous on the SiO{sub 2} substrate. 8. Cu-ZSM-5, Cu-ZSM-11, and Cu-ZSM-12 Catalysts for Direct NO Decomposition DEFF Research Database (Denmark) Kustova, Marina; Kustov, Arkadii; Christiansen, Sofie E. 2006-01-01 Cu-ZSM-5 has for many years been recognized as a unique catalyst for direct NO decomposition. Here, it is discovered that both Cu-ZSM-11 and Cu-ZSM-12 are about twice as active as Cu-ZSM-5. This difference is attributed to the active sites located almost exclusively in the straight zeolite pores... 9. Possible mechanism of superconductivity in CuO-Cu interface CERN Document Server Amelin, I I 2002-01-01 The CuO two-dimensional lattice consisting of the Cu sup 2 sup + and O sup 1 sup - ions which form the narrow partially filled two-dimensional zone is apparently formed in the CuO-Cu interface on the copper oxide surface. In this case formation of local electron pairs (LEP) is possible in the plane oxygen subsystem due to the Shubin-Vonsovskii conditions. In this approximation rough evaluation of the LEP formation temperature results in the T* approx 10 sup 4 K value. The temperature of the initial Bose-Einstein condensation by the concentration in the interface layer n approx 1.6 x 10 sup 2 sup 0 cm sup - sup 3 and the carriers effective mass m* approx m sub e may have the value of T sub c approx 1000 K. The obtained evaluation of the T sub c temperature corresponds by the values order to the experimental value 10. The influence of dietary Cu and diabetes on tissue sup 67 Cu retention kinetics in rats Energy Technology Data Exchange (ETDEWEB) Uriu-Hare, J.Y.; Rucker, R.B.; Keen, C.L. (Univ. of California, Davis (United States)) 1991-03-11 Compared to controls, diabetes results in higher plasma, liver and kidney Cu concentrations. Since alterations in Cu metabolism may be associated with diabetic pathology, the authors investigated how Cu metabolism is affected by diabetes and dietary Cu intake. Nondiabetic and STZ diabetic rats were fed Cu suppl. or Cu def. diets for 5 wks. Rats were intubated with 28 {mu}Ci {sup 67}Cu and killed after 8, 16, 24, 32, 64, or 128 h. There were marked effects of both diet and diabetes on {sup 67}Cu metabolism. Independent of diabetes, deficient rats had a higher % of retained {sup 67}Cu, in liver, plasma, RBC, muscle, spleen, brain, lung, uterus, and intestine than adequate Cu rats. Independent of dietary Cu, diabetic rats had a lower % of retained {sup 67}Cu in liver, plasma, RBC, muscle, spleen, lung, bone, pancreas, skin, uterus and heart than controls. Differential effects were noted for kidney; adequate Cu diabetic rats had a higher % of retained {sup 67}Cu than all other groups. Marked effects of both diet and diabetes were evident when tissue Cu turnover was examined. Compared to Cu suppl. rats, Cu def. rats had a slower turnover of {sup 67}Cu, in liver, plasma, intestine, pancreas, eye, brain, muscle, spleen, lung and heart. Diabetic rats had a slower turnover of {sup 67}Cu than nondiabetic rats in liver, plasma, intestine, pancreas, eye, kidney, RBC and uterus. The data imply that a focus on Cu metabolism with regard to cellular Cu trafficking and pathology may be warranted. 11. Copper tin sulfide (CTS) absorber thin films obtained by co-evaporation: Influence of the ratio Cu/Sn Energy Technology Data Exchange (ETDEWEB) Robles, V., E-mail: victor.robles@ciemat.es; Trigo, J.F.; Guillén, C.; Herrero, J. 2015-09-05 Highlights: • Copper tin sulfide (CTS) thin films were grown by co-evaporation at different Cu/Sn atomic ratios. • Smooth Cu{sub 2}SnS{sub 3} layers with large grains are obtained at Cu/Sn ⩾ 1.5 and T ⩾ 350 °C. • At 450 °C, the cubic Cu{sub 2}SnS{sub 3} phase changes to tetragonal phase. • Cu{sub 2}SnS{sub 3} presents suitable optical and electrical properties for use as photovoltaic absorbers. - Abstract: Copper tin sulfide thin films have been grown on soda-lime glass substrates from the elemental constituents by co-evaporation. The synthesis was performed at substrate temperatures of 350 °C and 450 °C and different Cu/Sn ratios, adjusting the deposition time in order to obtain thicknesses above 1000 nm. The evolution of the morphological, structural, chemical, optical and electrical properties has been analyzed as a function of the substrate temperature and the Cu/Sn ratio. For the samples with Cu/Sn ⩽ 1, Cu{sub 2}Sn{sub 3}S{sub 7} and Cu{sub 2}SnS{sub 3} have been observed by XRD. Increasing the Cu/Sn to 1.5, the Cu{sub 2}SnS{sub 3} phase was the majority, being the formation completed at Cu/Sn ratio around 2. The increment of the substrate temperature leads to a change of cubic structure to tetragonal of the Cu{sub 2}SnS{sub 3} phase. The chemical treatment with KCN was effective to eliminate CuS excess detected in the samples with Cu/Sn > 2.2. The samples with Cu{sub 2}SnS{sub 3} structure show a band gap energy increasing from 0.9 to 1.25 eV and an electrical resistivity decreasing from 7 ∗ 10{sup −2} Ω cm to 3 ∗ 10{sup −3} Ω cm when the Cu/Sn atomic ratio increases from 1.5 to 2.2. 12. Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis Science.gov (United States) Budak, Ferah; Bal, Salih Haldun; Tezcan, Gulcin; Akalın, Halis; Goral, Guher 2016-01-01 Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10–30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Four miRNAs were expressed in the chronic group but were not expressed in acute and control groups. Among these miRNAs, the expression level of miR-1238-3p was increased while miR-494, miR-6069, and miR-139-3p were decreased (p 2). These miRNAs have the potential to be markers for chronic cases. The differentially expressed miRNAs and their predicted target genes involved in endocytosis, regulation of actin cytoskeleton, MAPK signaling pathway, and cytokine-cytokine receptor interaction and its chemokine signaling pathway indicate their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human PBMC to clarify the mechanism of inveteracy in brucellosis. PMID:27722176 13. Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis. Science.gov (United States) Budak, Ferah; Bal, Salih Haldun; Tezcan, Gulcin; Akalın, Halis; Goral, Guher; Oral, Haluk Barbaros 2016-01-01 Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10-30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Four miRNAs were expressed in the chronic group but were not expressed in acute and control groups. Among these miRNAs, the expression level of miR-1238-3p was increased while miR-494, miR-6069, and miR-139-3p were decreased (p 2). These miRNAs have the potential to be markers for chronic cases. The differentially expressed miRNAs and their predicted target genes involved in endocytosis, regulation of actin cytoskeleton, MAPK signaling pathway, and cytokine-cytokine receptor interaction and its chemokine signaling pathway indicate their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human PBMC to clarify the mechanism of inveteracy in brucellosis. 14. 99Tcm-3P4-RGD2 SPECT显像对孤立性肺结节的诊断价值%Diagnostic value of solitary pulmonary nodules using 99Tcm-3P4-RGD2 scintigraphy Institute of Scientific and Technical Information of China (English) 孙昱; 王任婕; 高识; 纪铁凤; 纪滨; 贾兵; 戚良晨; 马庆杰 2013-01-01 Objective To investigate the value of 99Tcm-HYNIC-(poly-(ethylene glycol),PEG) 4-E (PEG4-c(RGDfK))2 (99Tcm-3P4-RGD2) SPECT imaging in the diagnosis of SPN by visual and semiquantitative analysis.Methods Twenty-one patients (13 men,8 women; age range 37-77 (58±11) years)with SPN observed on CT were analyzed prospectively.All patients underwent SPECT imaging after administration of 99Tcm-3P4-RGD2 with a dose of (939 ± 118) MBq.The gold standard was based on the histopathological diagnosis of the surgical samples from all recruited patients.The diagnostic performance of CT,SPECT visual and semiquantitative analysis was compared and analyzed with ROC curves.Immunohistochemistry was performed in part of the samples to obtain the information of integrin αvβ3 expression.Informed consent was obtained from all patients.Application of a new radiopharmaceutical was permitted and approved by the local independent Ethics Committee and the Institutional Review Board of the China-Japan Union Hospital.One-way analysis of variance,two-sample t test were used with SPSS 13.0.Results Among the 21 SPN patients,15 cases (71％) were diagnosed as malignant,and the other 6 (29％) were benign.T/NT ratio in malignant SPN was higher than that in benign SPN (1.87 ± 0.39 vs 1.41 ± 0.65),but with no statistical significance (t =2.01,P ＞ 0.05).The sensitivities for CT,SPECT visual and semiquantitative analysis were 80％ (12/15),100％ (15/15) and 100％ (15/15) respectively,and the specificity were all 4/6.The AUC was 0.811 (95％ CI 58％-95％) for CT,0.833 (95％ CI 61％-96％) for SPECT and 0.844 (95％ CI 62％-96％) for T/NT ratios,which showed no statistical significance (F =0.83,P ＞0.05).Immunohistochemistry confirmed αvβ3 expression in both the malignant and benign nodules which had uptake of 99Tcm-3P4-RGD2 in SPECT imaging.Conclusion SPECT visual and semiquantitative analysis with 99Tcm-3P4-RGD2 appears to have high sensitivity and consistency in diagnosis of SPN 15. O(3P)+HBr(DBr)反应的含时量子散射计算%Time-dependent Quantum Scattering Calculation of the O(3P)+HBr(DBr) Reaction Institute of Scientific and Technical Information of China (English) 左国平; 唐壁玉; 韩克利 2005-01-01 An exact three-dimensional time-dependent quantum wave packet was employed to calculate the O(3P) +HBr(DBr) reaction using a generalized London-Ering-Polanyi-Sato(LEPS) potential energy surface. The results showed that vibrational excitation is effective for the reaction, and rotational excitation has an orientational effect in definite energy range. The rate constants and the reaction cross sections for the title reactions have been computed, the calculated rate constants ko+HBr agreed well with experimental data. By comparing with relevant results, it can be found that the kinetic isotopic effects of the reaction are relatively obvious.%基于LEPS势能面,用三维含时量子波包法对O(3P)+HBr(DBr)反应进行了准确的动力学计算.计算的结果表明,振动激发对这个反应是有效的,而转动激发在某一能量范围内具有方位效应.计算得到了该反应的速率常数和反应截面,速率常数kO+HBr的计算值同实验值符合得很好.通过对相应结果的对比,可以发现这个反应具有比较明显的同位素效应. 16. MicroRNA-187-3p mitigates non-small cell lung cancer (NSCLC) development through down-regulation of BCL6 Energy Technology Data Exchange (ETDEWEB) Sun, Chengcao [Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan (China); Li, Shujun [Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan (China); Wuhan Hospital for the Prevention and Treatment of Occupational Diseases, 430071 Wuhan (China); Yang, Cuili; Xi, Yongyong; Wang, Liang; Zhang, Feng [Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan (China); Li, Dejia, E-mail: lodjlwhu@sina.com [Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan (China) 2016-02-26 Hsa-microRNA-187-3p (miR-187-3p) has recently been discovered having anticancer efficacy in different organs. However, the role of miR-187-3p on non-small cell lung cancer (NSCLC) is still ambiguous. In this study, we investigated the role of miR-187-3p on the development of NSCLC. The results indicated that miR-187-3p was significantly down-regulated in primary tumor tissues and very low levels were found in NSCLC cell lines. Ectopic expression of miR-187-3p in NSCLC cell lines significantly suppressed cell growth as evidenced by cell viability assay and colony formation assay, through inhibition of BCL6. In addition, miR-187-3p induced apoptosis, as indicated by concomitantly with up-regulation of the activities of caspase-3 and caspase-7, and inhibited cellular migration and invasiveness through inhibition of BCL6. Further, oncogene BCL6 was revealed to be a putative target of miR-187-3p, which was inversely correlated with miR-187-3p expression in NSCLC. Taken together, our results demonstrated that miR-187-3p played a pivotal role on NSCLC through inhibiting cell proliferation, migration, invasion, and promoting apoptosis by targeting oncogenic BCL6. 17. The effects of surface temperature on the gas-liquid interfacial reaction dynamics of O(3P)+squalane Science.gov (United States) Köhler, Sven P. K.; Allan, Mhairi; Kelso, Hailey; Henderson, David A.; McKendrick, Kenneth G. 2005-01-01 OH/OD product state distributions arising from the reaction of gas-phase O(3P) atoms at the surface of the liquid hydrocarbon squalane C30H62/C30D62 have been measured. The O(3P) atoms were generated by 355 nm laser photolysis of NO2 at a low pressure above the continually refreshed liquid. It has been shown unambiguously that the hydroxyl radicals detected by laser-induced fluorescence originate from the squalane surface. The gas-phase OH/OD rotational populations are found to be partially sensitive to the liquid temperature, but do not adapt to it completely. In addition, rotational temperatures for OH/OD(v'=1) are consistently colder (by 34±5 K) than those for OH/OD(v'=0). This is reminiscent of, but less pronounced than, a similar effect in the well-studied homogeneous gas-phase reaction of O(3P) with smaller hydrocarbons. We conclude that the rotational distributions are composed of two different components. One originates from a direct abstraction mechanism with product characteristics similar to those in the gas phase. The other is a trapping-desorption process yielding a thermal, Boltzmann-like distribution close to the surface temperature. This conclusion is consistent with that reached previously from independent measurements of OH product velocity distributions in complementary molecular-beam scattering experiments. It is further supported by the temporal profiles of OH/OD laser-induced fluorescence signals as a function of distance from the surface observed in the current experiments. The vibrational branching ratios for (v'=1)/(v'=0) for OH and OD have been found to be (0.07±0.02) and (0.30±0.10), respectively. The detection of vibrationally excited hydroxyl radicals suggests that secondary and/or tertiary hydrogen atoms may be accessible to the attacking oxygen atoms. 18. Circular RNA hsa_circ_0001564 facilitates tumorigenesis of osteosarcoma via sponging miR-29c-3p. Science.gov (United States) Li, Ji-Feng; Song, Yu-Ze 2017-08-01 Circular RNAs are a novel type of non-coding RNAs generated from back splicing, which has been verified to mediate multiple tumorigenesis. However, the role of circular RNA in osteosarcoma is still unclear. In this study, we preliminarily screened the circular RNAs expression profiles in osteosarcoma and investigated the potential regulation mechanism. The circular RNAs expression profiles in osteosarcoma were screened using circular RNA microarray analysis, and results showed that there were 1152 circular RNAs upregulated and 915 circular RNAs downregulated in tumor tissue compared to adjacent tissue. Hsa_circ_0001564, located at 5q35.3 and its associated gene symbol is CANX, was one of the significantly overexpressed circular RNAs in osteosarcoma tissue, as well as in osteosarcoma cell lines. In functional experiments, hsa_circ_001564 knockdown significantly suppressed the proliferation activity, induced cell-cycle arrest in G0/G1 phase, and promoted apoptosis in HOS and MG-63 cells. Subsequently, we explored the probable mechanism of hsa_circ_001564, and fortunately, bioinformatics analysis revealed that miR-29c-3p contained the complementary binding region with hsa_circ_0001564, which was confirmed by dual-luciferase reporter assay. Moreover, rescue experiments illustrated that miR-29c-3p could reverse the oncogenesis effect of hsa_circ_001564. Our study discovers that hsa_circ_0001564 acts as miR-29c-3p sponge to mediate the tumorigenicity, which could act as a potential biomarker for the osteosarcoma and provide a novel insight for competing endogenous RNA mechanism in osteosarcoma. 19. Dynamics of carbon-hydrogen and carbon-methyl exchanges in the collision of 3P atomic carbon with propene Science.gov (United States) Lee, Shih-Huang; Chen, Wei-Kan; Chin, Chih-Hao; Huang, Wen-Jian 2013-11-01 We investigated the dynamics of the reaction of 3P atomic carbon with propene (C3H6) at reactant collision energy 3.8 kcal mol-1 in a crossed molecular-beam apparatus using synchrotron vacuum-ultraviolet ionization. Products C4H5, C4H4, C3H3, and CH3 were observed and attributed to exit channels C4H5 + H, C4H4 + 2H, and C3H3 + CH3; their translational-energy distributions and angular distributions were derived from the measurements of product time-of-flight spectra. Following the addition of a 3P carbon atom to the C=C bond of propene, cyclic complex c-H2C(C)CHCH3 undergoes two separate stereoisomerization mechanisms to form intermediates E- and Z-H2CCCHCH3. Both the isomers of H2CCCHCH3 in turns decompose to C4H5 + H and C3H3 + CH3. A portion of C4H5 that has enough internal energy further decomposes to C4H4 + H. The three exit channels C4H5 + H, C4H4 + 2H, and C3H3 + CH3 have average translational energy releases 13.5, 3.2, and 15.2 kcal mol-1, respectively, corresponding to fractions 0.26, 0.41, and 0.26 of available energy deposited to the translational degrees of freedom. The H-loss and 2H-loss channels have nearly isotropic angular distributions with a slight preference at the forward direction particularly for the 2H-loss channel. In contrast, the CH3-loss channel has a forward and backward peaked angular distribution with an enhancement at the forward direction. Comparisons with reactions of 3P carbon atoms with ethene, vinyl fluoride, and vinyl chloride are stated. 20. Excitation of the low lying vibrational levels of H2O by O(3P) as measured on Spacelab 2 Science.gov (United States) Meyerott, R. E.; Swenson, G. R.; Schweitzer, E. L.; Koch, D. G. 1994-01-01 The data from the infrared telescope (IRT), which was flown on space shuttle Challenger Spacelab 2 mission (July 1985), were originally reported by Koch et al. (1987) as originating from near orbital emissions, primarily H2O. In this study, analysis of this data was extended to determine the collisional cross sections for the excitation of the low lying vibrational levels of H2O, present in the orbiter cloud, by atmospheric O(3P). The evaluation of the contribution to the measured signal from solar excitation and ram O excitation of outgassing H2O permits the determination of the H2O column density and the excitation cross section of the (101) level at an O(3P) velocity of approximately 7.75 km/s. Contributions to the radiation in the 1.7-3.0 micron band by transitions from the (100), (001), and multiquantum excited levels are discussed. The findings of the study are (1) the IRT data for the 4.5-9.5 micron and the nighttime data for the 1.7-3.0 micron sensors are consistent with being explained by collision excitation of H2O by O(3P), (2) diurnal variations of 4.5-9.5 micron intensities follow the model predicted O density for a full orbit, (3) daytime increases in the H2O cloud density were not evident, (4) the cross sections for the collisional excitation process are derived and compared to values computated by Johnson (1986) and Redmon et al. (1986), (5) theoretical investigation suggests greater than 60% of the radiation from H2O is a result of multiphoton emission resulting from collisional multiquanta excitation, and (6) the large daytime increase in the 1.7-3.0 micron intensity data suggests that O(+) may likely be instrumental in producing excited H2O(+) through charge exchange. 1. The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo. OpenAIRE Brown, J. T.; Bai, X.; Johnson, A. W. 2000-01-01 The yeast superkiller (SKI) genes were originally identified from mutations allowing increased production of killer toxin encoded by M "killer" virus, a satellite of the dsRNA virus L-A. XRN1 (SKI1) encodes a cytoplasmic 5'-exoribonuclease responsible for the majority of cytoplasmic RNA turnover, whereas SKI2, SKI3, and SKI8 are required for normal 3'-degradation of mRNA and for repression of translation of poly(A) minus RNA. Ski2p is a putative RNA helicase, Ski3p is a tetratricopeptide repe... 2. Attosecond time delay in the photoionization of Mn in the 3p \\rightarrow 3d giant resonance region CERN Document Server Dolmatov, V K; Deshmukh, P C; Manson, S T 2014-01-01 The dramatic effect of the 3p \\rightarrow 3d giant autoionization resonance on time delay of photoemission from the 3d and 4s valence subshells of the Mn atom is unraveled. Strong sensitivity of the time delay of the 4s photoemission to the final-state term of the ion-remainder [{\\rm Mn^{+}}(4s^{1},^{5}S)$vs.${\\rm Mn^{+}}(4s^{1},^{7}S)] is discovered. The features of time delay uncovered in Mn photoionization are expected to be general properties of transition-metal atoms and ions. The "spin-polarized" random phase approximation with exchange was employed in the study. 3. Intrinsic product polarization and branch ratio in the S(1D, 3P)+HD reaction on three electronic states Science.gov (United States) Li, Lin; Dong, Shunle 2016-09-01 The intrinsic product polarization and intramolecular isotope effect of the S(1D, 3P)+HD reaction have been investigated on both the lowest singlet state (1A‧) and the triplet state (3A‧ and 3A″) potential energy surfaces by using quasi-classical trajectory and quantum mechanical methods. The calculations indicate that intramolecular isotope effects are different on the three electronic states. The stereodynamics study shows that the P(θr) distributions, P(ϕr) distributions, and polarization-dependent differential cross sections (PDDCSs) (00) are sensitive to mass factor and the product angular momentum vectors are not only aligned but also oriented. 4. Chiral gels derived from secondary ammonium salts of (1R,3S-(+-camphoric acid Directory of Open Access Journals (Sweden) Tapas Kumar Adalder 2010-09-01 Full Text Available In order to have access to chiral gels, a series of salts derived from (1R,3S-(+-camphoric acid and various secondary amines were prepared based on supramolecular synthon rationale. Out of seven salts prepared, two showed moderate gelation abilities. The gels were characterized by differential scanning calorimetry, table top rheology, scanning electron microscopy, single crystal and powder X-ray diffraction. Structure property correlation based on X-ray diffraction techniques remain inconclusive indicating that some of the integrated part associated with the gelation phenomena requires a better understanding. 5. [Construction and application of special analysis database of geoherbs based on 3S technology]. Science.gov (United States) Guo, Lan-ping; Huang, Lu-qi; Lv, Dong-mei; Shao, Ai-juan; Wang, Jian 2007-09-01 In this paper,the structures, data sources, data codes of "the spacial analysis database of geoherbs" based 3S technology are introduced, and the essential functions of the database, such as data management, remote sensing, spacial interpolation, spacial statistics, spacial analysis and developing are described. At last, two examples for database usage are given, the one is classification and calculating of NDVI index of remote sensing image in geoherbal area of Atractylodes lancea, the other one is adaptation analysis of A. lancea. These indicate that "the spacial analysis database of geoherbs" has bright prospect in spacial analysis of geoherbs. 6. Internationally Recommended Best Practices in Transportation Financing Public-Private Partnerships (P3s) DEFF Research Database (Denmark) Martin, Lawrence; Lawther, Wendell; Hodge, Graeme 2013-01-01 sector partner designs-builds-finances-operates- maintains (DBFOM) a transportation infrastructure asset (road, highway, bridge, tunnel, etc.) with an emphasis on financing. Under this type of arrangement, the private sector partner is primarily responsible for securing all or substantially all...... internationally recommended best practices in transportation financing P3s. Based upon the study findings, a checklist in then proposed that can be used by U.S. state and local governments to assess their current or proposed transportation financing P3 policies and procedures. The article makes a contribution... 7. First Observation of Upsilon(3S) --> tau tau and Tests of Lepton Universality in Upsilon Decays CERN Document Server Besson, D; Cronin-Hennessy, D; Gao, K Y; Gong, D T; Hietala, J; Kubota, Y; Klein, T; Lang, B W; Poling, R; Scott, A W; Smith, A; Zweber, P; Dobbs, S; Metreveli, Z V; Seth, K K; Tomaradze, A G; Ernst, J; Severini, H; Dytman, S A; Love, W; Savinov, V; Aquines, O; Li, Z; López, A; Mehrabyan, S S; Méndez, H; Ramírez, J; Huang, G S; Miller, D H; Pavlunin, V; Sanghi, B; Shipsey, I P J; Xin, B; Adams, G S; Anderson, M; Cummings, J P; Danko, I; Napolitano, J; He, Q; Insler, J; Muramatsu, H; Park, C S; Thorndike, E H; Yang, F; Coan, T E; Gao, Y S; Liu, F; Artuso, M; Blusk, S; Butt, J; Horwitz, N; Li, J; Menaa, N; Mountain, R; Nisar, S; Randrianarivony, K; Redjimi, R; Sia, R; Skwarnicki, T; Stone, S; Wang, J C; Zhang, K; Csorna, S E; Bonvicini, G; Cinabro, D; Dubrovin, M; Lincoln, A; Asner, D M; Edwards, K W; Briere, R A; Brock, I; Chen, J; Ferguson, T; Tatishvili, G T; Vogel, H; Watkins, M E; Rosner, J L; Adam, N E; Alexander, J P; Berkelman, K; Cassel, D G; Duboscq, J E; Ecklund, K M; Ehrlich, R; Fields, L; Galik, R S; Gibbons, L; Gray, R; Gray, S W; Hartill, D L; Heltsley, B K; Hertz, D; Jones, C D; Kandaswamy, J; Kreinick, D L; Kuznetsov, V E; Mahlke-Krüger, H; Meyer, T O; Onyisi, P U E; Patterson, J R; Peterson, D; Pivarski, J; Riley, D; Ryd, A; Sadoff, A J; Schwarthoff, H; Shi, X; Stroiney, S; Sun, W M; Wilksen, T; Weinberger, M; Athar, S B; Patel, R; Potlia, V; Yelton, J; Rubin, P; Cawlfield, C; Eisenstein, B I; Karliner, I; Kim, D; Lowrey, N; Naik, P; Sedlack, C; Selen, M; White, E J; Wiss, J; Shepherd, M R 2007-01-01 Using data collected with the CLEO-III detector at the CESR e+e- collider, we report on a first observation of the decay of the Upsilon(3S) to tau tau, and precisely measure the ratio of branching fractions of Upsilon(nS),n=1,2,3 to tau tau and mu mu final states, finding agreement with expectations from lepton universality. We derive absolute branching fractions for these decays, and also set a limit on the influence of a low mass CP-odd Higgs boson in the decay of the Upsilon(1S). 8. Spectroscopy of the hydrogen 1 S -3 S transition with chirped laser pulses Science.gov (United States) Yost, D. C.; Matveev, A.; Grinin, A.; Peters, E.; Maisenbacher, L.; Beyer, A.; Pohl, R.; Kolachevsky, N.; Khabarova, K.; Hänsch, T. W.; Udem, Th. 2016-04-01 We identify a systematic present in two-photon direct frequency comb spectroscopy (DFCS) which is a result of chirped laser pulses and is a manifestation of the first-order Doppler effect. We carefully analyze this systematic and propose methods for its mitigation within the context of our measurement of the hydrogen 1 S -3 S transition. We also report on our determination of the absolute frequency of this transition, which is comparable to a previous measurement using continuous-wave spectroscopy [O. Arnoult et al., Eur. Phys. J. D 60, 243 (2010), 10.1140/epjd/e2010-00249-6], but was obtained with a different experimental method. 9. Formation and dissolution kinetics of Al(OH)3 (s) in synthetic freshwater solutions Science.gov (United States) Lydersen, E.; Salbu, B.; PolèO, A. B. S.; Muniz, I. P. 1991-03-01 The precipitation of Al in aqueous solutions can be described as a two-step process. When acidic inorganic Al solutions (pH 4.5) were titrated with NaOH to pH levels between 5.5 and 6.0, an amorphous Al(OH)3 (s) phase was formed instantaneously. During the first 5 min, the apparent half time for the reduction in dissolved Al species (t1/2) was 0.162 ± 0.07 hours (n = 4). The decrease of dissolved Al species continued during the following 24 hours, but at a far slower rate (t1/2 = 55.6 ± 25 hours (n = 4)). The highest precipitation rates were found in the solution of highest pH, and at approximately identical pH, the highest rate was found in the solution of highest temperature. The dissolution of amorphous Al(OH)3 (s) can also be described as a two-step process. When the amorphous Al solutions (pH between 5.5 and 6.0) were acidified to pH about 4.5 (HCl), the initial dissolution was relatively fast, but not as instantaneous as in the first step of precipitation. The apparent t1/2 after 5 min was estimated to be 0.65 ± 0.12 hours (n = 4). During the first 2.67 hours (160 min) the dissolution step could be described by the equation C(t) = C0e-0.45t, i.e., t1/2 = 1.53 ± 0.60 hours (n = 20) according to which the solid Al phase should dissolve totally (97%) during 7.65 hours. However, after 24 hours of storage 40 to 50% of the original Al(OH)3 (s) remained in the solid phase. This is explained by the presence of a far lower dissolution rate in a second step, i.e., the remaining Al(OH)3 (s) was less soluble. The average dissolution rate for the rest of the 24-hour period (21.33 hours) could be described by the exponential function C(t) = C0e-0.0095t, i.e., t1/2 = 73.2 ± 23.4 hours (n = 4). This implies that a total dissolution of Al (97%) will occur after 15.3 ± 4.9 days. The implications of these findings are discussed with reference to the current geochemical models pretending to simulate and predict aluminum chemistry in soils and surface runoff. 10. Cu metabolism in the liver. Science.gov (United States) McArdle, H J; Bingham, M J; Summer, K; Ong, T J 1999-01-01 This paper has, given some idea of our concepts of the processes involved in the transport of Cu across cell membranes in the liver, which we have summarised in Fig 1. Cu(II)His2 is reduced to Cu(I). This is transported across the membrane, re-oxidised, either before or after binding to glutathione (Freedman et al., 1989) or HAH1 (Klomp et al., 1997), binds to SAHH, and donates Cu(II) to the ATPase. It is very interesting that cells which are very diverse from an evolutionary point of view still use very similar methods to handle the metal. Whether regulation of transport is also the sam remains to be seen. We would guess that, although there will be strong similarities, there will also be very significant differences, reflecting the different environments seen by different tissues in mammalian cells and given the different requirements of the tissues. 11. Cu influence on hens weight Science.gov (United States) Afonina, I. A.; Kleptsyna, E. S.; Petukhov, V. L.; Patrashkov, S. A. 2003-05-01 Copper plays an important part in living being bodies. But, both high and low Cu levels may cause human and animal diseases. Some East Siberia areas are characterized by Cu pollution [1]. 5 group of hens were formed: 1 - control, 2-5 - experimental. For a month the hens from experimental groups were drunk with water where Cu content was 5, 10, 20 and 30 times higher than the upper limits (UL). Group 1 - 3 hens' weight was almost the same during the experiment. Weight decrease (from 2020 to 1656 g) was detected in group 4 (20 UL) for the first half a month. All the hens of group 4 except for 3 hens were died for the last 2 weeks. In group 5 (30 UL) all the hens died after 2 ... 14 days. Thus, high Cu concentrations (20 ... 30 UL) cause hens' weight reduction of and their death. 12. Effect of grain orientations of Cu seed layers on the growth of -oriented nanotwinned Cu National Research Council Canada - National Science Library Liu, Chien-Min; Lin, Han-Wen; Lu, Chia-Ling; Chen, Chih 2014-01-01 .... It is found that densely-packed nanotwinned Cu (nt-Cu) can be grown by pulsed electroplating on the strong -oriented Cu seed layer without a randomly-oriented transition layer between the nt-Cu and the Cu seed layer... 13. Thermodynamic studies on LnCoO{sub 3}(s) (Ln = Dy, Ho) by solid-state electrochemical cells Energy Technology Data Exchange (ETDEWEB) Patil, Abhay [Product Development Section, Bhabha Atomic Research Centre, Trombay, Mumbai 400085 (India); Dash, Smruti [Product Development Section, Bhabha Atomic Research Centre, Trombay, Mumbai 400085 (India)], E-mail: smruti@barc.gov.in; Parida, S.C.; Venugopal, V. [Product Development Section, Bhabha Atomic Research Centre, Trombay, Mumbai 400085 (India) 2009-01-05 Ternary oxides, DyCoO{sub 3}(s) and HoCoO{sub 3}(s) have been synthesized by citrate-nitrate gel combustion method and characterized by X-ray powder diffraction method. The standard molar Gibbs energies of formation of DyCoO{sub 3}(s) and HoCoO{sub 3}(s) have been measured using solid oxide galvanic cell technique employing yttria-stabilized zirconia (YSZ) and calcia-stabilized zirconia (CSZ) as solid electrolyte tubes, respectively. The standard molar Gibbs energies of formation of DyCoO{sub 3}(s) and HoCoO{sub 3}(s) were calculated from the measured e.m.f. data and are given as: {delta}{sub f}G{sub m}{sup o}(DyCoO{sub 3},s,T)kJmol{sup -1}({+-}4)=-1211.3+0.2449{sup *}(TK),(1013K{<=}T{>=}1167K), {delta}{sub f}G{sub m}{sup o}(HCoO{sub 3},s,T)kJmol{sup -1}({+-}4)=-1237.8+0.2590{sup *}(TK).(964K{<=}T{>=}1102K), A set of self consistent thermodynamic functions for LnCoO{sub 3}(s) (Ln = La, Nd, Sm, Eu, Gd, Dy, Ho) has been computed from available experimental data in the literature. 14. PI3P 和 NO 在 UV-B 诱导蚕豆气孔关闭中的关系%The interactions of PI3P and NO in UV-B-induced stomatal closure of broad bean Institute of Scientific and Technical Information of China (English) 樊彩明; 王静; 胡洁; 贺军民 2015-01-01 By stomatal bioassay and measurement of endogenous nitric oxide (NO)level in guard cells,the interactions of NO and phosphatidylinositol 3-phosphate (PI3P)[the product of phos-phatidylinositol 3-kinase (PI3K)]in UV-B-induced stomatal closure were studied in the epidermal strips of abaxial surface of broad bean(Vicia faba L.)leaves.The results showed that both the NO production in guard cells and stomatal closure induced by UV-B were significantly inhibited by PI3K inhibitors wortmannin (WM)and LY294002 (LY).Meanwhile,exogenous NO-relea-sing compound sodium nitroprusside (SNP)could completely reverse the inhibitory effect of LY and WM on the UV-B-induced stomatal closure,and WM and LY could not inhibit exogenous SNP-induced stomatal closure of broad bean.These results indicate that PI3P acts upstream of NO in the signal transduction pathway of UV-B-induced stomatal closure of broad bean.%以蚕豆（Vicia faba L．）叶片下表皮为材料，结合气孔开度分析和保卫细胞内源一氧化氮（NO）水平的测定，研究了 NO 和磷脂酰肌醇3-激酶（PI3K）的催化产物磷脂酰肌醇3-磷酸（PI3P）在紫外线 B（UV-B）诱导气孔关闭中的关系。结果显示：UV-B 辐射诱导蚕豆保卫细胞 NO 产生和气孔关闭的效应能被 PI3K 抑制剂沃曼青霉素（WM）和 LY294002（LY）显著抑制。同时，外源 NO释放剂硝普钠（SNP）处理能完全逆转 WM 和 LY 对 UV-B 诱导气孔关闭的抑制效应，而 WM 和LY 却不能抑制外源 SNP 诱导蚕豆气孔关闭的效应。结果说明，在 UV-B 诱导蚕豆气孔关闭的信号转导途径中 PI3P 的作用在 NO 上游。 15. White light generation from Dy3+-doped ZnO-B2O3-P2O5 glasses Science.gov (United States) Jayasimhadri, M.; Jang, Kiwan; Lee, Ho Sueb; Chen, Baojiu; Yi, Soung-Soo; Jeong, Jung-Hyun 2009-07-01 Dysprosium doped ZnO-B2O3-P2O5 (ZBP) glasses were prepared by a conventional melt quenching technique in order to study the luminescent properties and their utility for white light emitting diodes (LEDs). X-ray diffraction spectra revealed the amorphous nature of the glass sample. The present glasses were characterized by infrared and Raman spectra to evaluate the vibrational features of the samples. The emission and excitation spectra were reported for the ZBP glasses. Strong blue (484 nm) and yellow (574 nm) emission bands were observed upon various excitations. These two emissions correspond to the F49/2→H615/2 and F49/2→H613/2 transitions of Dy3+ ions, respectively. Combination of these blue and yellow bands gives white light to the naked eye. First time, it was found that ZnO-B2O3-P2O5 glasses efficiently emit white light under 400 and 454 nm excitations, which are nearly match with the emissions of commercial GaN blue LEDs and InGaN LED, respectively. CIE chromaticity coordinates also calculated for Dy3+: ZBP glasses to evaluate the white light emission. 16. Yeast Agp2p and Agp3p function as amino acid permeases in poor nutrient conditions. Science.gov (United States) Schreve, James L; Garrett, Jinnie M 2004-01-16 The gene AGP2 and the ORF YFL055w (here named AGP3) are classified as members of the yeast amino acid permease gene family. Analysis of the growth of multiply-mutant strains in which these genes are disrupted shows that both encode permeases capable of supplying branched chain, and other, amino acids as nitrogen source. Both Agp2p and Agp3p are low affinity permeases for leucine (Kmapp 0.2-0.5 mM) and are expressed at lower levels than other permeases on all media tested. Thus, it appears that these two permeases can function as low affinity, relatively non-specific, permeases with redundant functions in the cell. Transcription of AGP2 and AGP3 is very low but is increased in cells lacking other functional general amino acid permeases (Gap1p or Agp1p). These results suggest Agp2p and Agp3p function in amino acid transport when nitrogen sources are limiting and/or other permeases are inactive. 17. The Cu II Spectrum Directory of Open Access Journals (Sweden) Alexander Kramida 2017-02-01 Full Text Available New wavelength measurements in the vacuum ultraviolet (VUV, ultraviolet and visible spectral regions have been combined with available literature data to refine and extend the description of the spectrum of singly ionized copper (Cu II. In the VUV region, we measured 401 lines using a concave grating spectrograph and photographic plates. In the UV and visible regions, we measured 276 lines using a Fourier-transform spectrometer. These new measurements were combined with previously unpublished data from the thesis of Ross, with accurate VUV grating measurements of Kaufman and Ward, and with less accurate older measurements of Shenstone to construct a comprehensive list of ≈2440 observed lines, from which we derived a revised set of 379 optimized energy levels, complemented with 89 additional levels obtained using series formulas. Among the 379 experimental levels, 29 are new. Intensities of all lines observed in different experiments have been reduced to the same uniform scale by using newly calculated transition probabilities (A-values. We combined our calculations with published measured and calculated A-values to provide a set of 555 critically evaluated transition probabilities with estimated uncertainties, 162 of which are less than 20%. 18. Identification of an amphipathic helix important for the formation of ectopic septin spirals and axial budding in yeast axial landmark protein Bud3p. Science.gov (United States) Guo, Jia; Gong, Ting; Gao, Xiang-Dong 2011-03-08 Correct positioning of polarity axis in response to internal or external cues is central to cellular morphogenesis and cell fate determination. In the budding yeast Saccharomyces cerevisiae, Bud3p plays a key role in the axial bud-site selection (axial budding) process in which cells assemble the new bud next to the preceding cell division site. Bud3p is thought to act as a component of a spatial landmark. However, it is not clear how Bud3p interacts with other components of the landmark, such as the septins, to control axial budding. Here, we report that overexpression of Bud3p causes the formation of small septin rings (∼1 µm in diameter) and arcs aside from previously reported spiral-like septin structures. Bud3p closely associates with the septins in vivo as Bud3p colocalizes with these aberrant septin structures and forms a complex with two septins, Cdc10p and Cdc11p. The interaction of Bud3p with the septins may involve multiple regions of Bud3p including 1-858, 850-1220, and 1221-1636 a.a. since they all target to the bud neck but exhibit different effects on septin organization when overexpressed. In addition, our study reveals that the axial budding function of Bud3p is mediated by the N-terminal region 1-858. This region shares an amphipathic helix (850-858) crucial for bud neck targeting with the middle portion 850-1103 involved in the formation of ectopic septin spirals and rings. Interestingly, the Dbl-homology domain located in 1-858 is dispensable for axial bud-site selection. Our findings suggest that multiple regions of Bud3p ensure efficient targeting of Bud3p to the bud neck in the assembly of the axial landmark and distinct domains of Bud3p are involved in axial bud-site selection and other cellular processes. 19. miR-187-3p inhibits the metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by targeting S100A4. Science.gov (United States) Dou, Changwei; Liu, Zhikui; Xu, Meng; Jia, Yuli; Wang, Yufeng; Li, Qing; Yang, Wei; Zheng, Xin; Tu, Kangsheng; Liu, Qingguang 2016-10-28 miR-187-3p, a novel cancer-related microRNA, was previously reported to play promoting or suppressive roles in different malignancies. However, the expression level, biological function, and underlying mechanisms of miR-187-3p in hepatocellular carcinoma (HCC) remain unknown. This study demonstrated that miR-187-3p was significantly down-regulated in HCC tissues and cell lines, and was associated with advanced TNM stage and metastasis in HCC. Functional studies confirmed that miR-187-3p could inhibit the metastasis of HCC both in vitro and in vivo. Moreover, we proved that miR-187-3p could prevent the epithelial-mesenchymal transition (EMT) of HCC cells. Mechanically, S100A4 was a direct downstream target of miR-187-3p, and mediated the functional influence of miR-187-3p in HCC. Furthermore, miR-187-3p and S100A4 expression was evidently correlated with adverse clinical features and poor prognosis of HCC. Lastly, we showed that hypoxia was responsible for the significantly decreased level of miR-187-3p in HCC, and miR-187-3p was involved in the promoting effects of hypoxia on the metastasis and EMT of HCC cells. Taken together, miR-187-3p inhibits the metastasis and EMT in HCC by targeting S100A4. miR-187-3p can serve as a prognostic indicator and a promising therapeutic target for HCC patients. 20. The Copernicus Climate Change Service (C3S): A European Answer to Climate Change Science.gov (United States) Thepaut, Jean-Noel 2016-04-01 Copernicus is the European Commission's flagship Earth observation programme that delivers freely accessible operational data and information services. ECMWF has been entrusted to operate two key parts of the Copernicus programme, which will bring a consistent standard to the measurement, forecasting and prediction of atmospheric conditions and climate change: • The Copernicus Atmosphere Monitoring Service, CAMS, provides daily forecasts detailing the makeup composition of the atmosphere from the ground up to the stratosphere. • The Copernicus Climate Change Service (C3S) (in development) will routinely monitor and analyse more than 20 essential climate variables to build a global picture of our climate, from the past to the future, as well as developing customisable climate indicators for relevant economic sectors, such as energy, water management, agriculture, insurance, health…. C3S has now taken off and a number of proof-of-concept sectoral climate services have been initiated. This paper will focus on the description and expected outcome of these proof-of-concept activities as well as the definition of a roadmap towards a fully operational European Climate Change Service. 1. Equation of State of Fe3S and Limits on the Sulfur Content of Earth's Core Science.gov (United States) Campbell, A.; Mattillion, A.; Bausch, H.; Tecklenburg, S.; Fischer, R. A.; Chidester, B.; Prakapenka, V. 2016-12-01 Sulfur is a common component of protoplanetary cores, as represented by iron meteorites, and it is likely to be a significant alloying component with iron in Earth's core as well, along with silicon, oxygen, and perhaps other light elements. Cosmochemical limits on the sulfur content of Earth's core, based on the relative volatilities of sulfur and other elements, are weakened by the observation that this approach fails to accurately predict the sulfur content in iron meteorite parent bodies. To better understand the geophysical consequences of sulfur addition to Earth's core, we report equation of state measurements of Fe3S to pressures and temperatures exceeding 140 GPa and 2000 K, using synchrotron X-ray diffraction in a laser heated diamond anvil cell. New room temperature measurements are also reported, improved by the use of a neon pressure medium. With this P-V-T equation of state for Fe3S, along with an assumed 2% density change upon melting and a 4000 K core-mantle boundary temperature, the PREM density in the outer core can be matched with 14 wt% sulfur, which should be considered an upper bound because of the likely additional presence of other light elements. 2. An Integrated 3S and Historical Materials Analysis of the Keriya Paleoriver, NW China Science.gov (United States) Luo, Lei; Wang, Xinyuan; Cai, Heng 2014-03-01 Combining analysis of 3S (RS, GIS and GPS) and historical materials (historical records, ancient map and academic and literary writings) allows mapping of the Keriya Paleoriver of Southern Xinjiang, NW China. Keriya Paleoriver, one of the ancient Four Green Corridors which passes through the Taklimakan Desert from south to north in the Tarim Basin, recorded changes of the climate-environment in the ancient Silk Road of the region. According to the archaeological data, historical materials and paleoclimates information, its eco-environment and climate have had great changes since the 1.09Ma B.P., especially during the last 2,000 years, which has led to many famous ancient cities to be abandoned and the route of the ancient Silk Road to be moved southward. Using RS (optical and radar imagery), GIS (mapping and spatial analysis) and GPS (study area investigation), we mapped a major paleodrainage system of Keriya River, which have linked the Kunlun Mountains to the Tienshan Mountains through the Taklimakan Desert, possibly as far back as the early Pleistocene. This study illustrates the capability of the 3S and historical materials, in mapping the Keriya Paleoriver drainage networks and archaeological study on the ancient Silk Road. 3. MiR-338-3p targets pyruvate kinase M2 and affects cell proliferation and metabolism of ovarian cancer OpenAIRE Zhang, Yuting; Shi, Bing; Chen, Jiang; Hu, Lina; Zhao, Chunquan 2016-01-01 MiR-338-3p is down-regulated in cancer, which inhibits cancer cell proliferation, metastasis, and increases chemosensitivity, but its functions in ovarian cancer remains unknown. The present study aims to identify the miR-338-3p targeted genes and to investigate the associated regulatory mechanisms in ovarian cancer cell proliferation and metabolism. Our results demonstrated miR-338-3p expression was down-regulated in most of ovarian cancer tissues and cell lines. Restoration of miR-338-3p ex... 4. miR-625-3p regulates oxaliplatin resistance by targeting MAP2K6-p38 signalling in human colorectal adenocarcinoma cells DEFF Research Database (Denmark) Rasmussen, Mads Heilskov; Lyskjær, Iben; Jersie-Christensen, Rosa Rakownikow; 2016-01-01 Oxaliplatin resistance in colorectal cancers (CRC) is a major medical problem, and predictive markers are urgently needed. Recently, miR-625-3p was reported as a promising predictive marker. Herein, we show that miR-625-3p functionally induces oxaliplatin resistance in CRC cells, and identify the...... as one likely mechanism of oxaliplatin resistance. Our study shows that miR-625-3p induces oxaliplatin resistance by abrogating MAP2K6-p38-regulated apoptosis and cell cycle control networks, and corroborates the predictive power of miR-625-3p.... 5. Highly dynamic coordination behavior of Pn ligand complexes towards "naked" Cu(+) cations. Science.gov (United States) Fleischmann, Martin; Welsch, Stefan; Peresypkina, Eugenia V; Virovets, Alexander V; Scheer, Manfred 2015-10-01 Reactions of Cu(+) containing the weakly coordinating anion [Al{OC(CF3 )3 }4 ](-) with the polyphosphorus complexes [{CpMo(CO)2 }2 (μ,η(2) :η(2) -P2 )] (A), [CpM(CO)2 (η(3) -P3 )] (M=Cr(B1), Mo (B2)), and [Cp*Fe(η(5) -P5 )] (C) are presented. The X-ray structures of the products revealed mononuclear (4) and dinuclear (1, 2, 3) Cu(I) complexes, as well as the one-dimensional coordination polymer (5 a) containing an unprecedented [Cu2 (C)3 ](2+) paddle-wheel building block. All products are readily soluble in CH2 Cl2 and exhibit fast dynamic coordination behavior in solution indicated by variable temperature (31) P{(1) H} NMR spectroscopy. 6. The effect of dietary Cu and diabetes on indices of Cu nutriture in the rat Energy Technology Data Exchange (ETDEWEB) Rucker, R.B.; Uriu-Hare, J.Y.; Tinker, D.; Keen, C.L. (Univ. of California, Davis (United States)) 1991-03-11 The uptake-retention of 67Cu is affected by dietary Cu and diabetes. Consequently, the functional activities of select enzymes and tissue Cu status were assessed. STZ-diabetic and control rats were fed Cu suppl. or def. diets. Rats were gavaged with 28 {mu}Ci 67Cu, and killed 8, 16, 24, 32, 64, or 128 h later. Diabetic rats were hyperphagic, hyperglycemic and hypoinsulinemic; with no effect of diet. Plasma ceruloplasmin activity (Cp) was lower in Cu def. rats; diabetic rats tended to have higher Cp than controls. Cu def. rats had low Cu levels in liver, kidney and plasma. Cu suppl. diabetic rats had higher liver and kidney Cu compared to Cu def. diabetic rats. Gel chromatography of liver showed that with time, there was a transfer of 67Cu from low to higher MW ligands. In nondiabetic rats, more 67Cu was associated with the higher MW ligands. The converse was observed for diabetic rats. There was no effect of diabetes on liver 67Cu localization. Diabetic rats had higher metallothionein (MT) concentrations in liver and kidney compared to controls Cu deficiency lowered MT values in both diabetic and control rats. CuZn SOD Cu activity was lowered with Cu def. and diabetes, while Mn SOD activity was similar among groups. Plasma lipid peroxide levels were lower in diabetic rats than controls. The results show that Cu metabolism is affected in diabetes, and the changes are functionally significant. 7. MicroRNA 142-3p mediates post-transcriptional regulation of D1 dopamine receptor expression. Directory of Open Access Journals (Sweden) Krishna E Tobón Full Text Available The D1 dopamine receptor subtype is expressed in the brain, kidney and lymphocytes. D1 receptor function has been extensively studied and the receptor has been shown to modulate a wide range of physiological functions and behaviors. The expression of D1 receptor is known to change during development, disease states and chronic treatment; however, the molecular mechanisms that mediate the changes in D1 receptor expression under these circumstances are not well understood. While previous studies have identified extracellular factors and signaling mechanisms regulating the transcription of D1 receptor gene, very little is known about other regulatory mechanisms that modulate the expression of the D1 receptor gene. Here we report that the D1 receptor is post-transcriptionally regulated during postnatal mouse brain development and in the mouse CAD catecholaminergic neuronal cell line. We demonstrate that this post-transcriptional regulation is mediated by a molecular mechanism involving noncoding RNA. We show that the 1277 bp 3'untranslated region of D1 receptor mRNA is necessary and sufficient for mediating the post-transcriptional regulation. Using deletion and site-directed mutagenesis approaches, we show that the D1 receptor post-transcriptional regulation is specifically mediated by microRNA miR-142-3p interacting with a single consensus binding site in the 1277 bp 3'untranslated region of D1 receptor mRNA. Inhibiting endogenous miR-142-3p in CAD cells increased endogenous D1 receptor protein expression levels. The increase in D1 receptor protein levels was biologically significant as it resulted in enhanced D1 receptor-mediated signaling, determined by measuring the activation of both, adenylate cyclase and, the dopamine- and cAMP-regulated phosphoprotein, DARPP-32. We also show that there is an inverse correlation between miR-142-3p levels and D1 receptor protein expression in the mouse brain during postnatal development. This is the first 8. The Copernicus Climate Change Service (C3S): Open Access to a Climate Data Store Science.gov (United States) Thepaut, Jean-Noel; Dee, Dick 2016-04-01 In November 2014, The European Centre for Medium-range Weather Forecasts (ECMWF) signed an agreement with the European Commission to deliver two of the Copernicus Earth Observation Programme Services on the Commission's behalf. The ECMWF delivered services - the Copernicus Climate Change Service (C3S) and Atmosphere Monitoring Service (CAMS) - will bring a consistent standard to how we monitor and predict atmospheric conditions and climate change. They will maximise the potential of past, current and future earth observations - ground, ocean, airborne, satellite - and analyse these to monitor and predict atmospheric conditions and in the future, climate change. With the wealth of free and open data that the services provide, they will help business users to assess the impact of their business decisions and make informed choices, delivering a more energy efficient and climate aware economy. These sound investment decisions now will not only stimulate growth in the short term, but reduce the impact of climate change on the economy and society in the future. C3S is in its proof of concept phase and through its Climate Data Store will provide • global and regional climate data reanalyses; • multi-model seasonal forecasts; • customisable visual data to enable examination of wide range of scenarios and model the impact of changes; • access to all the underlying data, including climate data records from various satellite and in-situ observations. In addition, C3S will provide key indicators on climate change drivers (such as carbon dioxide) and impacts (such as reducing glaciers). The aim of these indicators will be to support European adaptation and mitigation policies in a number of economic sectors. At the heart of the Service is the provision of open access to a one stop shop (the Climate Data Store) of climate data and modelling, analysing more than 20 Essential Climate Variables to build a global picture of our past, present and future climate and developing 9. Phonon spectral densities of Cu surfaces: Application to Cu(211) Science.gov (United States) Mărinică, M.-C.; Raşeev, G.; Smirnov, K. S. 2001-05-01 Power phonon spectra of vicinal stepped surfaces of Cu(211) have been calculated using a molecular dynamics method combined with a semiempirical potential. The potential is based on an analytic form of inverse powers proposed by Finnis and Sinclair with the parametrization of Sutton and Chen. One of the four independent parameters of the potential was rescaled to reproduce the bulk phonon spectrum of Cu while retaining other properties of the bulk Cu close to the experimental values. Using this potential, we calculated the power surface phonon spectra, projection of the spectra at the high-symmetry points of surface Brillouin zone (SBZ), and the mean square displacements (MSD's) of atoms of the Cu(211) surface. The calculated projected phonon spectra at Γ¯ and at two new SBZ points (at X¯ and Y¯) compare favorably with experiment and theory when available. The MSD of the Cu(211) surface is also well reproduced and its temperature dependence shows that anharmonicity of the atomic motion becomes important above 200 K. 10. Significantly altered expression of miR-511-3p and its target AKT3 has negative prognostic value in human prostate cancer. Science.gov (United States) Zhang, Fan; Wu, Zhongjun 2017-09-01 In this study, we assessed the expression and functions of microRNA-511-3p (miR-511-3p) in human prostate cancer (CaP). Gene expressions of miR-511-3p in CaP cells and human CaP tumors were assessed by qPCR. In VCaP and PC3 cells, miR-511-3p was overexpressed by lentivirus. The functions of miR-511-3p upregulation in regulating in vitro cancer proliferation, migration and in vivo cancer growth were assessed by MTT, transwell and transplantation assays, respectively. Downstream target gene of miR-511-3p, AKT3, was verified by dual-luciferase activity and qPCR assays. AKT3 was then overexpressed in miR-511-3p-upregulated CaP cells to assess its functions in miR-511-3p-mediated cancer regulation. MiR-511-3p is significantly downregulated in CaP cell lines, and human CaP tumors. MiR-511-3p was further downregulated in T3/T4-staged CaP tumors and closely correlated with shorter overall survival among CaP patients. In VCaP and PC3 cells, lentiviral-induced miR-511-3p upregulation was acting as a tumor suppressor by inhibiting in vitro cancer proliferation, migration and in vivo transplantation. Human AKT3 gene was confirmed to be the downstream target of miR-511-3p in CaP. In miR-511-3p-upregulated VCaP and PC3 cells, forced-overexpression of AKT3 reversed the tumor suppressive effects of miR-511-3p in CaP. MiR-511-3p may serve as a prognostic factor and tumor suppressor in CaP, very likely through inverse regulation of its downstream target gene of AKT3. Copyright © 2017. Published by Elsevier B.V. 11. Investigation of vacuum properties of CuCrZr alloy for high-heat-load absorber Science.gov (United States) Shueh, C.; Chan, C. K.; Chang, C. C.; Sheng, I. C. 2017-01-01 The Taiwan Photon Source (TPS) uses high-heat-load (HHL) absorbers to protect downstream ultrahigh-vacuum chambers from overheating. In this work, we propose to use the CuCrZr alloy (ASTM C18150) for the HHL absorber body and the ConFlat flanges. We use the throughput method to measure the thermal outgassing rate and a helium leak detector to verify the vacuum seal between the CuCrZr alloy and stainless-steel flanges. The measured outgassing rate of the CuCrZr alloy was 5.8×10-10 Pa m/s after 72 h of pumping and decreased to 2.0 × 10-10 Pa m/s after 100 h of pumping. The leak rate through the vacuum seal between a CuCrZr flange and a stainless-steel flange was less than 1 × 10-10 Pa m3/s even after mounting and unmounting the flanges ten times and baking them at 250 °C. These results indicate that CuCrZr alloy is suitable for integrating HHL components with ConFlat CuCrZr flanges for the absorption of the synchrotron radiation generated by the TPS. 12. Research of Interaction Between Zn Based Solders and Cu, Al Substrates Directory of Open Access Journals (Sweden) Prach Michal 2014-06-01 Full Text Available The paper deals with the study of interaction between Cu, Al substrates (purity 5N and ZnAl4, ZnAg6Al6 zinc solders for higher application temperatures. Soldering was performed with power ultrasound in the air without flux application at temperature 420 °C. Acting time of ultrasonic vibration was 3 s and ultrasound frequency was 40 kHz. Soldered joints were assessed by optical light microscopy and EDX microanalysis. Intermetallic layers (IM CuZn4 and Cu5Zn8 were formed at the Cu/ZnAl4 boundary. The βZn-αAl mechanical mixture was formed at the Al/ZnAl4 boundary. AgZn3 and Cu5Zn8 IM layers were formed at the Cu/ZnAg6Al6 boundary, and mechanical mixture of βZn-αAl and AgZn3 intermetallic mixture were formed at the boundary Al/ZnAg6Al6. 13. 78 FR 69943 - Anthropomorphic Test Devices; Q3s 3-Year-Old Child Side Impact Test Dummy, Incorporation by... Science.gov (United States) 2013-11-21 ... depth and the waist circumference (both larger in the Q3s). As compared to a human, the Q3s torso is... 161 173 174 +1 Chest depth 122 122 151 +24 Chest circumference, axilla 508 505 523 +4 Waist... restraints to limit the risk of head and chest injury to children in a side impact. We are considering... 14. Kinetics and thermodynamic behavior of WO{sub 3} and WO{sub 3}:P thin films Energy Technology Data Exchange (ETDEWEB) Avellaneda, Cesar O.; Bulhoes, Luis O.S. [Laboratorio Interdisciplinar de Eletroquimica e Ceramica-LIEC, Departamento de Quimica, Universidade Federal de Sao Carlos, C.P. 676, CEP 13565-905, Sao Carlos-SP (Brazil) 2006-03-06 There is a considerable interest in the research and development of materials and devices, that can be used for optical switching of large-scale glazings. Several potential switching technologies are available for glazings, including those based on electrochromic, thermochromic and photochromic phenomena. One of the most promising technologies for optical switching devices is electrochromism (EC). In order to improve the electrochromic properties of tungsten oxide, we have investigated the effect of phosphorous insertion on the electrochromic behavior of oxide films prepared by the sol-gel process. The kinetics and thermodynamics of electrochemical intercalation of lithium into Li{sub x}WO{sub 3} and Li{sub x}WO{sub 3}:P films prepared by the sol-gel process were investigated. The standard Gibbs energy for lithium intercalation was calculated. The chemical diffusion coefficients, D, of lithium intercalation into oxide, were measured by galvanostatic intermittent titration technique (GITT), as functions of the depth of lithium intercalation. (author) 15. Neanderthal introgression at chromosome 3p21.31 was under positive natural selection in East Asians. Science.gov (United States) Ding, Qiliang; Hu, Ya; Xu, Shuhua; Wang, Jiucun; Jin, Li 2014-03-01 Studies of the Neanderthal and Denisovan genomes demonstrate archaic hominin introgression in Eurasians. Here, we present evidence of Neanderthal introgression within the chromosome 3p21.31 region, occurring with a high frequency in East Asians (ranging from 49.4% to 66.5%) and at a low frequency in Europeans. We also detected a signal of strong positive selection in this region only in East Asians. Our data indicate that likely candidate targets of selection include rs12488302-T and its associated alleles--among which four are nonsynonymous, including rs35455589-G in HYAL2, a gene related to the cellular response to ultraviolet-B irradiation. Furthermore, suggestive evidence supports latitude-dependent selection, implicating a role of ultraviolet-B. Interestingly, the distribution of rs35455589-G suggests that this allele was lost during the exodus of ancestors of modern Eurasians from Africa and reintroduced to Eurasians from Neanderthals. 16. Spin-orbit and rotational couplings in radiative association of C(3P) and N(4S) atoms. Science.gov (United States) Antipov, Sergey V; Gustafsson, Magnus; Nyman, Gunnar 2011-11-14 The role of spin-orbit and rotational couplings in radiative association of C((3)P) and N((4)S) atoms is investigated. Couplings among doublet electronic states of the CN radical are considered, giving rise to a 6-state model of the process. The solution of the dynamical problem is based on the L(2) method, where a complex absorbing potential is added to the Hamiltonian operator in order to treat continuum and bound levels in the same manner. Comparison of the energy-dependent rate coefficients calculated with and without spin-orbit and rotational couplings shows that the couplings have a strong effect on the resonance structure and low-energy baseline of the rate coefficient. 17. Photoconductivity of novel poly(N-vinyl)-3-[p-nitrophenylazo]carbazole/CdS-nanoparticle polymer composite Institute of Scientific and Technical Information of China (English) DING Li-yun; JIANG De-sheng; HUANG Jun; DAI Lei; LIU Cheng; WANG Jun-tao; LI Bin 2006-01-01 The photoconductive characteristic of the inorganic/organic hybridized polymer system is reported,in which a novel bi-functional photorefractive (PR) poly(N-vinyl)-3-[p-nitrophenylazo]carbazole (PVNPAK) serves as a polymeric charge-transporting and second-order nonliner optical matrix and quantum dots composed of surface passivated cadmium sulfide serve as a charge-generation sensitizer. The hybrid PVNPAK/CdS-nanoparticles polymer composites with different mass ratio of CdS to PVNPAK were prepared. The generation of photocurrent on illumination and photoconductive properties of the PVNPAK/CdS-nanoparticles polymer composites were studied. The results show that the addition of CdS nanoparticle as a photosensitizer can enhance the photoconductivity of the PVNPAK significantly because of the properties of the high quantum efficiency of photosensitization and high charge transport to conducting polymer. 18. Magnetic control of ultra-cold^6$Li and$^{174}$Yb($^3P_2$) atom mixtures with Feshbach resonances CERN Document Server Petrov, Alexander; Kotochigova, Svetlana 2015-01-01 We theoretically evaluate the feasibility to form magnetically-tunable Feshbach molecules in collisions between fermionic$^6$Li atoms and bosonic metastable$^{174}$Yb($^3$P$_2$) atoms. In contrast to the well-studied alkali-metal atom collisions, collisions with meta-stable atoms are highly anisotropic. Our first-principle coupled-channel calculation of these collisions reveals the existence of broad Feshbach resonances due to the combined effect of anisotropic-molecular and atomic-hyperfine interactions. In order to fit our predictions to the specific positions of experimentally-observed broad resonance structures \\cite{Deep2015} we optimized the shape of the short-range potentials by direct least-square fitting. This allowed us to identify the dominant resonance by its leading angular momentum quantum numbers and describe the role of collisional anisotropy in the creation and broadening of this and other resonances. 19. The C(3P) + NH3 reaction in interstellar chemistry: I. Investigation of the product formation channels CERN Document Server Bourgalais, Jeremy; Kailasanathan, Ranjith Kumar Abhinavam; Osborn, David L; Hickson, Kevin M; Loison, Jean-Christophe; Wakelam, Valentine; Goulay, Fabien; Picard, Sébastien D Le 2016-01-01 The product formation channels of ground state carbon atoms, C(3P), reacting with ammonia, NH3, have been investigated using two complementary experiments and electronic structure calculations. Reaction products are detected in a gas flow tube experiment (330 K, 4 Torr) using tunable VUV photoionization coupled with time of flight mass spectrometry. Temporal profiles of the species formed and photoionization spectra are used to identify primary products of the C + NH3 reaction. In addition, H-atom formation is monitored by VUV laser induced fluorescence from room temperature to 50 K in a supersonic gas flow generated by the Laval nozzle technique. Electronic structure calculations are performed to derive intermediates, transition states and complexes formed along the reaction coordinate. The combination of photoionization and laser induced fluorescence experiments supported by theoretical calculations indicate that in the temperature and pressure range investigated, the H + H2CN production channel represents ... 20. Ultrastable laser system for spectroscopy of the 1S0-3P0 clock transition in Sr atoms Science.gov (United States) Berdasov, O. I.; Gribov, A. Yu.; Belotelov, G. S.; Pal'chikov, V. G.; Strelkin, S. A.; Khabarova, K. Yu.; Kolachevsky, N. N.; Slyusarev, S. N. 2017-06-01 A laser system with a spectral linewidth less than 1 Hz for spectroscopy of the 1S0-3P0 clock transition in strontium atoms has been demonstrated. A semiconductor laser emitting at a wavelength of 698 nm was stabilised to an external high-finesse Fabry-Perot cavity with vibration and temperature compensation near the zero expansion point. After laser cooling to a temperature below 3 μK, 88Sr atoms were loaded into an optical lattice at a magic wavelength of 813 nm. The laser system was used to characterise the 88Sr clock transition by magnetically induced spectroscopy. The resonance spectral width was determined to be 130 ± 17 Hz, which corresponds to a quality factor of 3 × 1012. 1. Major weapon system environmental life-cycle cost estimating for Conservation, Cleanup, Compliance and Pollution Prevention (C3P2) Science.gov (United States) Hammond, Wesley; Thurston, Marland; Hood, Christopher 1995-01-01 The Titan 4 Space Launch Vehicle Program is one of many major weapon system programs that have modified acquisition plans and operational procedures to meet new, stringent environmental rules and regulations. The Environmental Protection Agency (EPA) and the Department of Defense (DOD) mandate to reduce the use of ozone depleting chemicals (ODC's) is just one of the regulatory changes that has affected the program. In the last few years, public environmental awareness, coupled with stricter environmental regulations, has created the need for DOD to produce environmental life-cycle cost estimates (ELCCE) for every major weapon system acquisition program. The environmental impact of the weapon system must be assessed and budgeted, considering all costs, from cradle to grave. The Office of the Secretary of Defense (OSD) has proposed that organizations consider Conservation, Cleanup, Compliance and Pollution Prevention (C(sup 3)P(sup 2)) issues associated with each acquisition program to assess life-cycle impacts and costs. The Air Force selected the Titan 4 system as the pilot program for estimating life-cycle environmental costs. The estimating task required participants to develop an ELCCE methodology, collect data to test the methodology and produce a credible cost estimate within the DOD C(sup 3)P(sup 2) definition. The estimating methodology included using the Program Office weapon system description and work breakdown structure together with operational site and manufacturing plant visits to identify environmental cost drivers. The results of the Titan IV ELCCE process are discussed and expanded to demonstrate how they can be applied to satisfy any life-cycle environmental cost estimating requirement. 2. Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates Directory of Open Access Journals (Sweden) Emmanuel M Papamichael 1999-01-01 Full Text Available In this study, the S1 - P1 and S3 - P3 interactions between papain and four synthetic peptide substrates were found as to be important. The values of Km were estimated as to be practically identical between these substrates; this latter is supporting the conclusions obtained by considering the estimated values of other kinetic parameters. Nevertheless, based on the estimated kcat and/or k cat/Km parameters of the used substrates, we concluded that an aromatic ring at the P3 position, and a positively charged side chain of the residue at the P1 position of the synthetic substrates were favored considerably their interaction with papain.Neste estudo, o S1 - P1 e S3 - P3, interações entre papaina e quatro substratos sintéticos de pepetídios foram considerados importantes. Os valores de Km foram estimados e são praticamente idênticos entre esses substratos; Isso dá suporte as conclusões obtidas, considerando os valores parâmetros cinéticos estimados. No obstante, baseou na estimação parâmetros kcat e/ou k cat /Km dos substratos utilizados. Se pode concluir que um anel aromático na posição P3, e uma corrente carregada positivamente da cadeia do resíduo na posição P1 dos substratos sintéticos favoreceram interação com a papaina. 3. Bonding and Site Preferences in the New Quasi-Binary Zr 2.7Hf 11.3P 9 Science.gov (United States) Kleinke, Holger; Franzen, Hugo F. 1998-03-01 The new quasi-binary Zr 2.7Hf 11.3P 9was synthesized by arc-melting of Zr, Hf, Co, and HfP in a ratio corresponding to the initial composition "Zr 2.25Hf 6.75Co 2P 4". Zr 2.7Hf 11.3P 9crystallizes in the Zr 14P 9structure type, which is unknown in the binary Hf/P system. The ideal orthorhombic lattice dimensions (space group Pnnm(No. 58), Z=4) were refined to a=16.640(7) Å, b=27.40(2) Å, c=3.619(1) Å, V=1650(2) Å 3. The structure consists of three-dimensional condensed one-, two-, and three-capped trigonal (Zr, Hf) 6P prisms, occurring with numerous short M- Mbonds ( M=Zr, Hf). Each of the 15 metal sites is statistically occupied by a mixture of Zr and Hf, which varies significantly from site to site. The Hf/Zr ratio in a given site depends on the M- Mand M-P interactions. The systematic increase of this ratio with increasing total bond order, as evaluated via Mulliken overlap populations and Pauling bond orders, can be understood based on the trend that Hf forms stronger M- Mand M-P bonds than Zr. As expected for a metal-rich phosphide, band structure calculations for the hypothetical "Hf 14P 9" carried out with the extended Hückel approximation result in a significant density of states at the Fermi level. 4. Fabrication and characterization of nanostructured Fe3S4, an isostructural compound of half-metallic Fe3O4 KAUST Repository Li, Peng 2015-06-10 High-purity, well-crystallized spinel Fe3S4 nanoplatelets were synthesized by the hydrothermal method, and the saturation magnetic moment of Fe3S4 was measured at 1.83 μB/f.u. The temperature-dependent resistivity of Fe3S4 was metallic-like for T < 180 K: room-temperature resistivity was measured at 7.711 × 103 μΩ cm. The anomalous Hall conductivity of Fe3S4 decreased with increasing longitudinal conductivity, in sharp contrast with the accepted theory of the anomalous Hall effect in a dirty-metal regime. Furthermore, negligible spin-dependent magnetoresistance was observed. Band structure calculations confirmed our experimental observations that Fe3S4 is a metal and not a half metal as expected. 5. Evidence of the quadrupolar ordering in DyPd{sub 3}S{sub 4} Energy Technology Data Exchange (ETDEWEB) Matsuoka, E. [Institute for Solid State Physics, University of Tokyo, Kashiwanoha, Kashiwa, Chiba (Japan)]. E-mail: eiichi@issp.u-tokyo.ac.jp; Kitagawa, J.; Ishikawa, M. [Institute for Solid State Physics, University of Tokyo, Kashiwanoha, Kashiwa, Chiba (Japan); Ohoyama, K. [Institute for Materials Research, Tohoku University, Katahira, Aoba-ku, Sendai, Miyagi (Japan); Yoshizawa, H. [Neutron Scattering Laboratory, Institute for Solid State Physics, University of Tokyo, Shirakata, Tokai, Ibaraki (Japan) 2001-12-03 By means of specific heat, magnetic susceptibility, and neutron powder diffraction experiments, we reinvestigated new samples of DyPd{sub 3}S{sub 4} synthesized by an improved method. We found a new phase transition at T{sub Q}=3.4 K above the two magnetic transitions around 1 K, which was characterized by a distinct peak in the specific heat and only a small anomaly in the magnetic susceptibility. The neutron powder diffraction experiment demonstrated neither new magnetic reflections nor evidence of a change of the crystal structure below T{sub Q}. It was furthermore found that the quartet crystalline-electric-field ground state is consistent with the result for the magnetic entropy deduced from the specific heat and that T{sub Q} increases with the external magnetic field. These results strongly imply that the new phase transition at T{sub Q} is due to antiferroquadrupolar ordering. (author) 6. Probabilistic risk analysis toward cost-effective 3S (safety, safeguards, security) implementation Science.gov (United States) Suzuki, Mitsutoshi; Mochiji, Toshiro 2014-09-01 Probabilistic Risk Analysis (PRA) has been introduced for several decades in safety and nuclear advanced countries have already used this methodology in their own regulatory systems. However, PRA has not been developed in safeguards and security so far because of inherent difficulties in intentional and malicious acts. In this paper, probabilistic proliferation and risk analysis based on random process is applied to hypothetical reprocessing process and physical protection system in nuclear reactor with the Markov model that was originally developed by the Proliferation Resistance and Physical Protection Working Group (PRPPWG) in Generation IV International Framework (GIF). Through the challenge to quantify the security risk with a frequency in this model, integrated risk notion among 3S to pursue the cost-effective installation of those countermeasures is discussed in a heroic manner. 7. Two-Dimensional Infrared (2DIR) Spectroscopy of the Peptide Beta3s Folding Science.gov (United States) Lai, Zaizhi; Preketes, Nicholas K; Jiang, Jun; Mukamel, Shaul; Wang, Jin 2013-01-01 Probing underlying free energy landscape, pathways, and mechanism is the key for understanding protein folding in theory and experiment. Recently time-resolved two-dimensional infrared (2DIR) with femtosecond laser pulses, has emerged as a promising tool for investigating the protein folding dynamics on faster timescales than possible by NMR. We have employed molecular dynamics simulations to compute 2DIR spectra of the folding process of a peptide, Beta3s. Simulated non-chiral and chiral 2DIR signals illustrate the variation of the spectra as the peptide conformation evolves along the free energy landscape. Chiral spectra show stronger changes than the non-chiral signals because cross peaks caused by the formation of the β-sheet are clearly resolved. Chirality-induced 2DIR may be used to detect the folding of β-sheet proteins with high spectral and temporal resolution. PMID:23956818 8. Helium 2 3S - 2 1S metrology at 1557 nm CERN Document Server Van Leeuwen, K A H 2006-01-01 An experiment is proposed to excite the 'forbidden' 1s2s 3S1 - 1s2s 1S0 magnetic dipole (M1) transition at 1557 nm in a collimated and slow atomic beam of metastable helium atoms. It is demonstrated that an excitation rate of 5000 /s can be realised with the beam of a 2W narrowband telecom fiber laser intersecting the atomic beam perpendicularly. A Doppler-limited sub-MHz spectroscopic linewidth is anticipated. Doppler-free excitation of 2% of trapped and cooled atoms may be realised in a one-dimensional optical lattice geometry, using the 2W laser both for trapping and spectroscopy. The very small (8 Hz) natural linewidth of this transition presents an opportunity for accurate tests of atomic structure calculations of the helium atom. A measurement of the 3He - 4He isotope shift allows for accurate determination of the difference in nuclear charge radius of both isotopes. 9. Immunologic Basis for Long HCDR3s in Broadly Neutralizing Antibodies against HIV-1 Directory of Open Access Journals (Sweden) Lei eYu 2014-06-01 Full Text Available A large panel of potent broadly neutralizing antibodies (bnAbs against HIV-1 has been reported in recent years, raising hope for the design of an effective vaccine based on epitopes recognized by these protective antibodies. However, many of these bnAbs contain the feature of long heavy chain complementarity-determining region 3 (HCDR3 sequences, which is viewed as an obstacle to the development of an HIV-1 vaccine targeting long HCDR3 bnAb responses. This mini-review summarizes the current literature and discusses the different potential Immunologic mechanisms for generating long HCDR3, including D-D fusion, VH replacement, long N region addition and skewed D-J genes usage, among which potential VH replacement products appear to be significant contributors. VH replacement occurs through RAG-mediated secondary recombination and contributes to the diversified naïve B cell repertoire. During VH replacement, a short stretch of nucleotides from previously rearranged VH genes is left within the newly formed HCDR3, thus elongating its length. Accumulating evidence suggests that long HCDR3s are present at significant numbers in the human mature naïve B cell repertoire and are primarily generated by recombination during B cell development. These new observations indicate that long HCDR3s, though in low frequency, are a normal feature of human antibody naïve repertoire and they appear to be selected to target conserved epitopes located in deep regions of the HIV-1 envelope trimer during HIV-1 infection. Therefore, the presence of long HCDR3 sequences should not necessarily be viewed as an obstacle to the development of an HIV-1 vaccine that promotes bnAb responses. 10. Cell Monitoring and Manipulation Systems (CMMSs based on Glass Cell-Culture Chips (GC3s Directory of Open Access Journals (Sweden) Sebastian M. Buehler 2016-06-01 Full Text Available We developed different types of glass cell-culture chips (GC3s for culturing cells for microscopic observation in open media-containing troughs or in microfluidic structures. Platinum sensor and manipulation structures were used to monitor physiological parameters and to allocate and permeabilize cells. Electro-thermal micro pumps distributed chemical compounds in the microfluidic systems. The integrated temperature sensors showed a linear, Pt1000-like behavior. Cell adhesion and proliferation were monitored using interdigitated electrode structures (IDESs. The cell-doubling times of primary murine embryonic neuronal cells (PNCs were determined based on the IDES capacitance-peak shifts. The electrical activity of PNC networks was detected using multi-electrode arrays (MEAs. During seeding, the cells were dielectrophoretically allocated to individual MEAs to improve network structures. MEA pads with diameters of 15, 20, 25, and 35 µm were tested. After 3 weeks, the magnitudes of the determined action potentials were highest for pads of 25 µm in diameter and did not differ when the inter-pad distances were 100 or 170 µm. Using 25-µm diameter circular oxygen electrodes, the signal currents in the cell-culture media were found to range from approximately −0.08 nA (0% O2 to −2.35 nA (21% O2. It was observed that 60-nm thick silicon nitride-sensor layers were stable potentiometric pH sensors under cell-culture conditions for periods of days. Their sensitivity between pH 5 and 9 was as high as 45 mV per pH step. We concluded that sensorized GC3s are potential animal replacement systems for purposes such as toxicity pre-screening. For example, the effect of mefloquine, a medication used to treat malaria, on the electrical activity of neuronal cells was determined in this study using a GC3 system. 11. Distal 3p duplication and terminal 7q deletion associated with nuchal edema and cyclopia in a fetus and a review of the literature Directory of Open Access Journals (Sweden) Chih-Ping Chen 2015-06-01 Conclusion: Simultaneous occurrence of 7q deletion and 3p duplication can be associated with alobar holoprosencephaly. For the couple with a parental translocation involving 7q and 3p, prenatal ultrasound should include a detailed investigation of central nervous system anomalies. 12. THE FUNGAL HYDROPHOBIN SC3P SELF-ASSEMBLES AT THE SURFACE OF AERIAL HYPHAE AS A PROTEIN MEMBRANE CONSTITUTING THE HYDROPHOBIC RODLET LAYER NARCIS (Netherlands) WOSTEN, HAB; ASGEIRSDOTTIR, SA; KROOK, JH; DRENTH, JHH; WESSELS, JGH 1994-01-01 The Schizophyllum commune hydrophobin Sc3p is a small, hydrophobic, cysteine-rich protein involved in the formation of aerial hyphae. Using an antibody against purified Sc3p we found that the hydrophobin is secreted into the medium at the spices of growing submerged hyphae but in emerging aerial hyp 13. Reactions of R(2)P-P(SiMe(3))Li with [(R'(3)P)(2)PtCl(2)]. A general and efficient entry to phosphanylphosphinidene complexes of platinum. Syntheses and structures of [(eta(2)-P=(i)Pr(2))Pt(p-Tol(3)P)(2)], [(eta(2)-P=(t)Bu(2))Pt(p-Tol(3)P)(2)], [{eta(2)-P=(N(i)Pr(2))(2)}Pt(p-Tol(3)P)(2)] and [{(Et(2)PhP)(2)Pt}(2)P(2)]. Science.gov (United States) Domańska-Babul, Wioleta; Chojnacki, Jaroslaw; Matern, Eberhard; Pikies, Jerzy 2009-01-07 The reactions of lithium derivatives of diphosphanes R(2)P-P(SiMe(3))Li (R = (t)Bu, (i)Pr, Et(2)N and (i)Pr(2)N) with [(R'(3)P)(2)PtCl(2)] (R'(3)P = Et(3)P, Et(2)PhP, EtPh(2)P and p-Tol(3)P) proceed in a facile manner to afford side-on bonded phosphanylphosphinidene complexes of platinum [(eta(2)-P=R(2))Pt(PR'(3))(2)]. The related reactions of Ph(2)P-P(SiMe(3))Li with [(R'(3)P)(2)PtCl(2)] did not yield [(eta(2)-P=PPh(2))Pt(PR'(3))(2)] and resulted mainly in the formation of [{(R'(3)P)(2)Pt}(2)P(2)], Ph(2)P-PLi-PPh(2), (Me(3)Si)(2)PLi and (Me(3)Si)(3)P. Crystallographic data are reported for the compounds [(eta(2)-P=R(2))Pt(p-Tol(3)P)(2)] (R = (t)Bu, (i)Pr, ((i)Pr(2)N)(2)P) and for [{(Et(2)PhP)(2)Pt}(2)P(2)]. 14. MiR-291b-3p Induces Apoptosis in Liver Cell Line NCTC1469 by Reducing the Level of RNA-binding Protein HuR Directory of Open Access Journals (Sweden) Jun Guo 2014-03-01 Full Text Available Background: There is increasing evidence that miRNAs are involved in cellular apoptosis. However, the specific role of miR-291b-3p in apoptosis has not been elucidated. In the present study, we investigated the effect of miR-291b-3p on NCTC1469 cell growth and apoptosis. Methods: Cell viability and apoptosis were examined in NCTC1469 cells transfected with miR-291b-3p mimics, inhibitor miRNA or negative control. Using computational miRNA target prediction databases, HuR was predicted as a target of miR-291b-3p. Luciferase assay, immunofluorescence and western blot were used to further explore the effects of miR-291b-3p on HuR expression. In addition, the effect of HuR on cell apoptosis was evaluated using a HuR-specific siRNA. Results: TNF-α-induced hepatocyte apoptosis was accompanied by enhanced expression of miR-291b-3p, suggesting that miR-291b-3p might contribute to the apoptotic process. Follow-up experiments showed that upregulation of miR-291b-3p decreased cell viability and induced NCTC1469 cell apoptosis. Additionally, similar to the activity of miR-519, which is another member of the same miRNA family, miR-291b-3p suppressed HuR translation through binding to the HuR coding region (CR. We further showed that the downregulation of HuR expression by miR-291b-3p was accompanied by reduced Bcl-2 expression. Moreover, knockdown of HuR also impaired Bcl-2 expression and increased the ratio of Bax/Bcl-2. More significantly, downregulation of miR-291b-3p failed to increase Bcl2 expression in NCTC1469 cells that were co-transfected with siRNA-HuR. Finally, inhibition of miR-291b-3p led to reduced apoptosis, while knockdown of HuR by siRNA promoted apoptosis, even in NCTC1469 cells that were co-transfected with the miR-291b-3p inhibitor. Conclusion: The current data suggested that miR-291b-3p contributed to NCTC1469 cell apoptosis by regulating the expression of HuR, which in turn increased Bcl-2 stability. 15. Speciation of Cu in MSWI bottom ash and its relation to Cu leaching Energy Technology Data Exchange (ETDEWEB) Arickx, S. [Department of Chemical Engineering, Katholieke Universiteit Leuven, W. de Croylaan 46, B-3001 Leuven (Belgium)], E-mail: stefanie.arickx@telenet.be; Gerven, T. van [Department of Chemical Engineering, Katholieke Universiteit Leuven, W. de Croylaan 46, B-3001 Leuven (Belgium); Boydens, E.; L' Hoest, P.; Blanpain, B. [Department of Metallurgy and Materials Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 44, B-3001 Leuven (Belgium); Vandecasteele, C. [Department of Chemical Engineering, Katholieke Universiteit Leuven, W. de Croylaan 46, B-3001 Leuven (Belgium)], E-mail: carlo.vandecasteele@cit.kuleuven.be 2008-12-15 In Flanders, recycling of bottom ash is mainly inhibited by the high leaching of Cu. Although it has been proved that dissolved organic C plays a major role in the Cu leaching, the possible role of inorganic Cu mineral speciation has never been experimentally examined. In this study the speciation of Cu is investigated using a combination of optical microscopy and electron microprobe -WDX/EDX. Several Cu species were determined. Metallic Cu (with or without an oxide shell), CuO and Cu{sub 2}O were the most abundant. These particles were most likely present in wire-like structures. Copper also occurred as alloy (brass, bronze, zamak), and was found frequently together with typical elements such as Ca, Cl and S. Finally, small metallic Cu particles seemed to be trapped in or precipitated on oxides and silicates. Based on this Cu speciation study, pure Cu minerals were selected and leached as a function of time. The solubility after equilibrium of all studied Cu minerals never exceeded 20 {mu}g/L (which equals 10% of the total Cu leaching). The effect of heating (2 h at 400 deg. C) on the speciation of Cu was investigated using the same combination of techniques. Results show that metallic Cu seemed to be converted to Cu oxide (mostly CuO) and that the particles were more porous after heating. These conclusions were verified by XRD analysis of the heated pure Cu minerals. After heating, the Cu minerals were also leached as a function of time, to study the impact on Cu leaching. Results indicate that their leaching had slightly increased in comparison with the non-heated Cu minerals. However, the major decrease in Cu leaching in heated bottom ash, more than neutralizes this effect and thus can be attributed to the destruction of organic matter and not to the (small) change in Cu speciation. 16. Thermochemistry of the Cu{sub 2}Se–In{sub 2}Se{sub 3} system Energy Technology Data Exchange (ETDEWEB) Ider, M., E-mail: muhsin.ider@usak.edu.tr [Department of Chemical Engineering, Usak University, 64200 Usak (Turkey); Pankajavalli, R. [Thermodynamics and Kinetics Division, Indira Gandhi Centre for Atomic Research, Kalpakkam 603102 (India); Zhuang, W. [General Research Institute for Nonferrous Metals, Grirem Advanced Materials Co., Ltd., Beijing 100088 (China); Shen, J.Y. [General Research Institute for Nonferrous Metals, Beijing 100088 (China); Anderson, T.J. [Department of Chemical Engineering, University of Florida, Gainesville, FL 32611 (United States) 2014-08-01 Highlights: • Solid state EMF experiments were employed to obtain Gibbs energy data of CuInSe{sub 2}. • The phase diagram data in the Cu{sub 2}Se–In{sub 2}Se{sub 3} pseudo-binary system were assessed. • ΔG{sub f}{sup ∘} CuInSe{sub 2} (α) ± 0.0003 = 0.0051T (K) − 220.92 kJ/mol (949–1044 K) was obtained. • ΔG{sub f}{sup ∘} CuInSe{sub 2} (δ) ± 0.0004 = −0.0043T (K) − 210.92 kJ/mol (1055–1150 K) was obtained. • T{sub trans} and, ΔH{sub trans}{sup ∘} for α to δ-CuInSe{sub 2} were determined to be 1064 K and 10.0 kJ/mol. - Abstract: Solid state electrochemical cells were employed to obtain standard Gibbs energy of formation of CuInSe{sub 2} as well as the temperature and enthalpy of the α to δ-CuInSe{sub 2} transformation in the Cu{sub 2}Se–In{sub 2}Se{sub 3} pseudo-binary system. The reversible EMF data of the following solid-state electrochemical cell were measured: Pt,In(l),In{sub 2}O{sub 3}(s)‖YSZ‖In{sub 2}O{sub 3}(s),Cu{sub 2}Se(s),Cu(s),CuInSe{sub 2}(αorδ),C,PtCell I The calculated standard molar Gibbs energy of formation of α and δ-CuInSe{sub 2} from measured data are given by ΔG{sub f}{sup ∘}CuInSe{sub 2}(α)±0.0003=0.0051T(K)-220.92kJ/mol(949–1044K) ΔG{sub f}{sup ∘}CuInSe{sub 2}(δ)±0.0004=-0.0043T(K)-210.92kJ/mol(1055–1150K) The α to δ phase transition temperature T{sub trans} and the enthalpy of transition ΔH{sub trans}{sup ∘} for CuInSe{sub 2} were determined to be 1064 K and 10.0 kJ/mol respectively. ΔS{sub trans}{sup ∘} was calculated as 9.4 J/mol K. The thermodynamic and phase diagram data in the Cu{sub 2}Se–In{sub 2}Se{sub 3} pseudo-binary system were critically assessed. A self consistent set of thermochemistry and phase diagram data was obtained with the help of measured data. The liquid phase along the Cu{sub 2}Se–In{sub 2}Se{sub 3} pseudo-binary was calculated with the Redlich–Kister model. The β-Cu{sub 1}In{sub 3}Se{sub 5} and γ-Cu{sub 1}In{sub 5}Se{sub 8} phases were 17. Phosphorus-Rich CuP2 Embedded in Carbon Matrix as a High-Performance Anode for Lithium-Ion Batteries. Science.gov (United States) Kim, Sang-Ok; Manthiram, Arumugam 2017-05-17 Phosphorus-rich CuP2 and its carbon composites have been investigated as an anode material for lithium-ion batteries. Through a facile, low-cost mechanochemical reaction, microsized composites composed of active CuP2 particles uniformly embedded in the carbon matrix have been successfully synthesized. Combined structural and electrochemical characterizations show that phosphorus-rich CuP2 undergoes irreversible reaction with lithium, giving metal-rich Cu3P and amorphous phosphorus at the end of the first cycle. Both Cu3P and phosphorus are reversibly formed in subsequent cycles, contributing to a high reversible capacity of >1000 mA h g(-1). By controlling the carbon content, the electrochemical reversibility and stability of CuP2 are greatly improved. The carbon composite demonstrates a remarkable lithium-storage capability in terms of a stable capacity of >720 mA h g(-1) over 100 cycles at 200 mA g(-1), a high initial Coulombic efficiency of ∼83%, and a good rate capability with a capacity of >637 mA h g(-1) at 1.6 A g(-1). The performance improvement is mainly associated with the formation of the conductive carbon network that offers high conductivity and fast reaction kinetics, as well as enhanced structural stability of CuP2 anode. 18. Interfacial tension studies between Fe-Cu-Ni sulfide and halo-norilsk basalt slag system Institute of Scientific and Technical Information of China (English) SU Shangguo; Jim Mungall; WANG Jian; GENG Ke 2005-01-01 The interfacial tension of the matte/halo-Norilsk basalt slag systems of FeS-Cu2S-Ni3S2 and FeO-FeS were investigated using the sessile drop technique. The results indicate that interfacial tension decreases with increasing copper and nickel contents in the matte of FeS-Cu2S-Ni3S2 system while it increases with increasing oxygen content in the matte of FeO-FeS system. It is inferred from these results that two conditions are critical for the formation of giant Cu-Ni sulfide deposits. One is that mafic-ultramafic parent magma of sulfide deposits should be rich in copper and nickel where due to the low interfacial tension, it is difficult to form sulfide droplet in the early stage of magma evolution. In other words, sulfide liquid conglomeration occurs more difficultly. The other condition is that the magma emplacement should be shallow; and a lot of faults occur in the magma emplacement field. Since oxygen content is high in the environment, interfacial tension is high, which helps sulfide liquid conglomeration and consequently Cu-Ni sulfide deposits form. 19. miR-373-3p Targets DKK1 to Promote EMT-Induced Metastasis via the Wnt/β-Catenin Pathway in Tongue Squamous Cell Carcinoma Science.gov (United States) Zhang, Hui; Wang, Cheng; Liang, Jianfeng; Chen, Guanhui; Li, Wenqing; Tang, Haikuo 2017-01-01 MicroRNAs (miRNAs) regulate gene expression and at the same time mediate tumorigenesis. miR-373-3p has diverse effects in tumors, but its role in tongue squamous cell carcinoma (TSCC) remains unknown. The purpose of this study is to determine the function of miR-373-3p in the progression of TSCC. Our results brought to light that miR-373-3p is markedly upregulated in clinical TSCC tissues compared with paired adjacent normal tissues and has significant correlation with a more aggressive TSCC phenotype in patients. Gain-of-function and loss-of-function studies revealed that ectopic miR-373-3p overexpression promoted the metastasis of TSCC cells. Notably, Wnt/β-catenin signaling was hyperactivated in TSCC cells overexpressing miR-373-3p, and this pathway was responsible for the epithelial-mesenchymal transition (EMT) induced by miR-373-3p. Furthermore, miR-373-3p directly targeted and suppressed Dickkopf-1 (DKK1), a negative regulator of the Wnt/β-catenin signaling cascade. These results demonstrate that, by directly targeting DKK1, miR-373-3p constitutively activated Wnt/β-catenin signaling, thus promoting the EMT-induced metastasis of TSCC. Taken together, our findings reveal a new regulatory mechanism for miR-373-3p and suggest that miR-373-3p might be a potential target in TSCC therapy. 20. Two PI 3-kinases and one PI 3-phosphatase together establish the cyclic waves of phagosomal PtdIns(3P critical for the degradation of apoptotic cells. Directory of Open Access Journals (Sweden) Nan Lu 2012-01-01 Full Text Available Phosphatidylinositol 3-phosphate (PtdIns(3P is a signaling molecule important for many membrane trafficking events, including phagosome maturation. The level of PtdIns(3P on phagosomes oscillates in two waves during phagosome maturation. However, the physiological significance of such oscillation remains unknown. Currently, the Class III PI 3-kinase (PI3K Vps34 is regarded as the only kinase that produces PtdIns(3P in phagosomal membranes. We report here that, in the nematode C. elegans, the Class II PI3K PIKI-1 plays a novel and crucial role in producing phagosomal PtdIns(3P. PIKI-1 is recruited to extending pseudopods and nascent phagosomes prior to the appearance of PtdIns(3P in a manner dependent on the large GTPase dynamin (DYN-1. PIKI-1 and VPS-34 act in sequence to provide overlapping pools of PtdIns(3P on phagosomes. Inactivating both piki-1 and vps-34 completely abolishes the production of phagosomal PtdIns(3P and disables phagosomes from recruiting multiple essential maturation factors, resulting in a complete arrest of apoptotic-cell degradation. We have further identified MTM-1, a PI 3-phosphatase that antagonizes the activities of PIKI-1 and VPS-34 by down-regulating PtdIns(3P on phagosomes. Remarkably, persistent appearance of phagosomal PtdIns(3P, as a result of inactivating mtm-1, blocks phagosome maturation. Our findings demonstrate that the proper oscillation pattern of PtdIns(3P on phagosomes, programmed by the coordinated activities of two PI3Ks and one PI 3-phosphatase, is critical for phagosome maturation. They further shed light on how the temporally controlled reversible phosphorylation of phosphoinositides regulates the progression of multi-step cellular events. 1. Diffusion welding process of submicron aluminium matrix composite Al2O3p/6061 Al%亚微米级Al2O3p/6061Al铝基复合材料扩散焊接工艺 Institute of Scientific and Technical Information of China (English) 刘黎明; 高振坤; 董长富; 韩文波 2004-01-01 以亚微米级Al2O3p/6061 Al铝基复合材料为对象,研究了直接扩散焊与采用中间层扩散焊两种工艺焊接铝基复合材料的特点、机理,分析了中间层对接头强度的影响规律.结果表明,在铝基复合材料液、固温度区间,存在"临界温度区域",在此温度区域进行直接扩散焊接时,通过液相基体金属的浸润,使得在扩散接合面中增强相-增强相接触转化为增强相-基体-增强相的有机结合,获得高质量焊接接头;进一步研究发现,在扩散接合面上采用合适的基体中间层同样可以将增强相-增强相接触转化为增强相-基体-增强相的有机结合,同时增大"临界温度区域"范围,接头性能更加稳定,接头变形量进一步减小(＜2%). 2. Measurement of the resonance parameters of the chi(1)(1**3P(1)) and chi(2)(1**3P(2)) states of charmonium formed in antiproton-proton annihilations Energy Technology Data Exchange (ETDEWEB) Andreotti, M.; Bagnasco, S.; Baldini, W.; Bettoni, D.; Borreani, G.; Buzzo, A.; Calabrese, R.; Cester, R.; Cibinetto, G.; Dalpiaz, P.; Garzoglio, G.; Gollwitzer, K.E.; Graham, M.; Hu, M.; Joffe, D.; Kasper, J.; Lasio, G.; Lo Vetere, M.; Luppi, E.; Macri, M.; Mandelkern, M.; /Fermilab /INFN, Ferrara /Ferrara U. /INFN, Genoa /Genoa U. /UC, Irvine 2005-03-01 The authors have studied the {sup 3}P{sub J} ({chi}{sub e}) states of charmonium in formation by antiproton-proton annihilations in experiment E835 at the Fermilab Antiproton Source. The authors report new measurements of the mass, width, and B({chi}{sub cJ} {yields} {bar p}p) x {Lambda}({chi}{sub eJ} {yields} J/{psi} + anything) for the {chi}{sub c1} and {chi}{sub c2} by means of the inclusive reaction {bar p}p {yields} {chi}{sub cJ} {yields} J/{psi} + anything {yields} (e{sup +}e{sup -}) + anything. Using the subsample of events where {chi}{sub cJ} {yields} {gamma} + J/{psi} {yields} {gamma} + (e{sup +}e{sup -}) is fully reconstructed, we derive B({chi}{sub cJ} {yields} {bar p}p) x {Lambda}({chi}{sub cJ} {yields} J/{psi} + {gamma}). They summarize the results of the E760 (updated) and E835 measurements of mass, width and B({chi}{sub cJ} {yields} {bar p}p){Lambda}({chi}{sub cJ} {yields} J/{psi} + {gamma}) (J = 0,1,2) and discuss the significance of these measurements. 3. miR-199a-3p targets CD44 and reduces proliferation of CD44 positive hepatocellular carcinoma cell lines Energy Technology Data Exchange (ETDEWEB) Henry, Jon C. [Department of Surgery, Ohio State University Medical Center, Columbus, OH (United States); Park, Jong-Kook; Jiang, Jinmai; Kim, Ji Hye [College of Pharmacy, Ohio State University, Columbus, OH (United States); Nagorney, David M.; Roberts, Lewis R. [Divisions of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, MN (United States); Banerjee, Soma [Center for Liver Research, School of Digestive and Liver Diseases, Institute of Post Graduate Medical Education and Research, Kolkata (India); Schmittgen, Thomas D., E-mail: Schmittgen.2@osu.edu [College of Pharmacy, Ohio State University, Columbus, OH (United States) 2010-12-03 Research highlights: {yields} miR-199a-3p targets CD44 in HCC. {yields} Proliferation and invasion are reduced by miR-199a-3p in CD44+ HCC. {yields} miR-199a-3p is reduced and CD44 protein is increased in HCC tissues. {yields} The duplex form of miR-199a-3p mimetic is required for activity. -- Abstract: Previous work by us and others reported decreased expression of miR-199a-3p in hepatocellular carcinoma (HCC) tissues compared to adjacent benign tissue. We report here a significant reduction of miR-199a-3p expression in 7 HCC cell lines. To determine if miR-199a-3p has a tumor suppressive role, pre-miR-199a-3p oligonucleotides were transfected into the HCC cell lines. Pre-miR-199a-3p oligonucleotide reduced cell proliferation by approximately 60% compared to control oligonucleotide in only two cell lines (SNU449 and SNU423); the proliferation of the other 5 treated cell lines was similar to control oligonucleotide. A pre-miR-199a-3p oligonucleotide formulated with chemical modifications to enhance stability while preserving processing, reduced cell proliferation in SNU449 and SNU423 to the same extent as the commercially available pre-miR-199a-3p oligonucleotide. Furthermore, only the duplex miR-199a-3p oligonucleotide, and not the guide strand alone, was effective at reducing cell viability. Since a CD44 variant was essential for c-Met signaling [V. Orian-Rousseau, L. Chen, J.P. Sleeman, P. Herrlich, H. Ponta, CD44 is required for two consecutive steps in HGF/c-Met signaling, Genes Dev. 16 (2002) 3074-3086] and c-Met is a known miR-199a-3p target, we hypothesized that miR-199a-3p may also target CD44. Immunoblotting confirmed that only the two HCC lines that were sensitive to the effects of pre-miR-199a-3p were CD44+. Direct targeting of CD44 by miR-199a-3p was confirmed using luciferase reporter assays and immunoblotting. Transfection of miR-199a-3p into SNU449 cells reduced in vitro invasion and sensitized the cells to doxorubicin; both effects were enhanced 4. Temperature-Dependent Kinetics Studies of the Reactions Br((sup 2)P3/2) + H2S yields SH + HBr and Br((sup 2)P3/2) + CH3SH yields CH3S + HBr. Heats of Formation of SH and CH3S Radicals Science.gov (United States) Nicovich, J. M.; Kreutter, K. D.; vanDijk, C. A.; Wine, P. H. 1997-01-01 Time resolved resonance fluorescence detection of Br(sup 2)P3/2) atom disappearance or appearance following 266-nm laser flash photolysis of CF2Br2/H2S/H2/N2, CF2Br2/CH3SH/H2/N2, Cl2CO/H2S/HBr/N2, and CH3SSCH3/HBr/H2/N2 mixtures has been employed to study the kinetics of the reactions Br((sup 2)P3/2) + H2S = SH + HBr (1,-1) and Br((sup2)P3/2) + CH3SH = CH3S + HBr (2, -2) as a function of temperature over the range 273-431K. Arrhenius expressions in units of 10(exp -12) cu cm/molecule/s which describe the results are k1 = (14.2 +/- 3.4) exp[(-2752 +/- 90)/T],(k-1) = (4.40 +/- 0.92) exp[(-971 +/- 73)/T],k(2) = (9.24 +/- 1.15) exp[(-386 +/- 41)/T], and k(-2) = (1.46 +/-0.21) exp[(-399 +/-41)/T; errors are 2 sigma and represent precision only. By examining Br((sup 2)P3/2) equilibrium kinetics following 355nm laser flash photolysis of Br2/CH3SH/H2/N2 mixtures, a 298 K rate coefficient of (1.7 +/- 0.5) x 10(exp -10) cu cm/molecule/s has been obtained for the reaction CH3S + Br2 yields CH3SBr + Br. To our knowledge, these are the first kinetic data reported for each of the reactions studied. Measured rate coefficients, along with known rate coefficients for similar radical + H2S, CH3SH, HBr,Br2 reactions are considered in terms of possible correlations of reactivity with reaction thermochemistry and with IP - EA, the difference between the ionization potential of the electron donor and the electron affinity of the electron acceptor. Both thermochemical and charge-transfer effects appear to be important in controlling observed reactivities. Second and third law analyses of the equilibrium data for reactions 1 and 2 have been employed to obtain the following enthalpies of reaction in units of kcal/mol: for reaction 1, Delta-H(298) = 3.64 +/- 0.43 and Delta-H(0) = 3.26 +/-0.45; for reaction 2, Delta-H(298) = -0.14 +/- 0.28 and Delta-H(0) = -0.65 +/- 0.36. Combining the above enthalpies of reaction with the well-known heats of formation of Br, HBr, H2S, and CH3SH gives the 5. Microfluidic reactor synthesis and photocatalytic behavior of Cu@Cu{sub 2}O nanocomposite Energy Technology Data Exchange (ETDEWEB) Xu, Lei, E-mail: xulei_kmust@aliyun.com [National Local Joint Laboratory of Engineering Application of Microwave Energy and Equipment Technology, Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093 (China); Mechanical Engineering, University of Washington, Seattle, WA 98195 (United States); Srinivasakannan, C. [Chemical Engineering Program, The Petroleum Institute, PO Box 253, Abu Dhabi (United Arab Emirates); Peng, Jinhui, E-mail: jhpeng@kmust.edu.cn [National Local Joint Laboratory of Engineering Application of Microwave Energy and Equipment Technology, Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093 (China); Yan, Mi [Faculty of Mathematical and Physical Sciences, University College London, London WC1E 6BT (United Kingdom); Zhang, Di [Mechanical Engineering, University of Washington, Seattle, WA 98195 (United States); Zhang, Libo [National Local Joint Laboratory of Engineering Application of Microwave Energy and Equipment Technology, Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093 (China) 2015-03-15 Highlights: • The Cu@Cu{sub 2}O nanocomposites were synthesized in microfluidic reactor followed by oxidation process. • The Cu@Cu{sub 2}O composite particle is on nanoscale exhibiting an open bicontinuous structure. • The amount of Cu{sub 2}O can be controlled by varying drying temperature. • The binary Cu@Cu{sub 2}O–H{sub 2}O{sub 2} systems exhibit an excellent photocatalyst for degradation methylene blue under UV irradiation. - Abstract: The Cu@Cu{sub 2}O nanocomposites were synthesized by solution-phase synthesis of Cu nanoparticles in microfluidic reactor at room temperature, followed by controlling the oxidation process. The size, morphology, elemental compositions, and the chemical composition on the surface of Cu@Cu{sub 2}O nanocomposite were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). Experimental results demonstrated that the surface of the Cu nanoparticles was oxidized to Cu{sub 2}O which serves as the shell of nanoparticle. The amount of Cu{sub 2}O can be controlled by varying the drying temperature. Additionally the binary Cu@Cu{sub 2}O nanocomposite along with H{sub 2}O{sub 2} exhibited its potential as an excellent photocatalyst for degradation of methylene blue (MB) under UV irradiation. 6. Mir-338-3p Mediates Tnf-A-Induced Hepatic Insulin Resistance by Targeting PP4r1 to Regulate PP4 Expression. Science.gov (United States) Dou, Lin; Wang, Shuyue; Sun, Libo; Huang, Xiuqing; Zhang, Yang; Shen, Tao; Guo, Jun; Man, Yong; Tang, Weiqing; Li, Jian 2017-01-01 Insulin resistance is a critical factor contributing to the pathogenesis of type 2 diabetes and other metabolic diseases. Recent studies have indicated that miR-338-3p plays an important role in cancer. Here, we investigated whether miR-338-3p mediates tumour necrosis factor-α (TNF-α)-induced hepatic insulin resistance. The activation of the insulin signalling pathway and the level of glycogenesis were examined in the livers of the db/db and high fat diet (HFD)-fed mice and in HEP1-6 cells transfected with miR-338-3p mimic or inhibitor. Computational prediction of microRNA target, luciferase assay and Western blot were used to assess the miR-338-3p target. Chromatin immunoprecipitation (ChIP) assay was used to determine the transcriptional regulator of miR-338-3p. miR-338-3p was down-regulated in the livers of the db/db, HFD-fed and TNF-α-treated C57BL/6J mice, as well as in mouse HEP1-6 hepatocytes treated with TNF-α. Importantly the down-regulation of miR-338-3p induced insulin resistance, as indicated by impaired glucose tolerance and insulin tolerance. Further research showed that the down-regulated miR-338-3p resulted in the impaired AKT/ glycogen synthase kinase 3 beta (GSl·Gβ) signalling pathway and glycogen synthesis. In contrast, hepatic over-expression of miR-338-3p rescued the TNF-α-induced insulin resistance. Moreover, protein phosphatase 4 regulator subunit 1 (PP4R1) was identified as a direct target of miR-338-3p that mediated hepatic insulin signalling by regulating protein phosphatase 4 (PP4). Finally we identified hepatic nuclear factor 4 alpha (HNF-4α) as the transcriptional regulator of miRNA-338-3p. Our studies provide novel insight into the critical role and molecular mechanism by which miR-338-3p is involved in TNF-α-induced hepatic insulin resistance. miR-338-3p might mediate TNF-α-induced hepatic insulin resistance by targeting PP4R1 to regulate PP4 expression. © 2017 The Author(s). Published by S. Karger AG, Basel. 7. MicroRNA-219-2-3p functions as a tumor suppressor in gastric cancer and is regulated by DNA methylation. Directory of Open Access Journals (Sweden) Huizi Lei Full Text Available BACKGROUND AIMS: Gastric cancer is the most frequent gastrointestinal tumor in adults and is the most lethal form of human cancer. Despite of the improvements in treatments, the underlying mechanism of gastric carcinogenesis is not well known. To define novel modulators that regulate susceptibility to tumorgenesis, we focused on miR-219-2-3p. METHODS: Quantitative RT-PCR was employed to investigate the level of miR-219-2-3p in gastric cancer (GC tissues (n = 113 and their matched adjacent normal tissues (n = 113. In vitro cell proliferation, apoptosis assays, cell migration, and invasion assays were performed to elucidate biological effects of miR-219-2-3p. Since silencing of miRNA by promoter CpG island methylation may be an important mechanism in tumorgenesis, GC cells were treated with 5-aza-2'-deoxycytidine and trichostatin A, and expression changes of miR-219-2-3p were subsequently examined by quantitative RT-PCR. Finally, the methylation status of CpG island upstream of miR-219-2-3p was analyzed by methylation-specific PCR in GC tissues (n = 22. RESULTS: miR-219-2-3p was down-regulated in GC and cell lines. In addition, the experiments documented the lower expression of miR-219-2-3p in GC specimens with higher grade and later stage tumors. Meanwhile, miR-219-2-3p exerted antiproliferative, proapoptotic, and antimetastatic roles and reduced levels of p-ERK1/2 in GC cells. Furthermore, 5-aza-2'-deoxycytidine and trichostatin A increased the expression (~2 fold of miR-219-2-3p in GC cells. By methylation-specific PCR, DNA methylation in the upstream region of miR-219-2-3p was detected in both adjacent normal tissues and cancer tissues. As expected, the methylation level was considerably higher in the miR-219-2-3p down-regulated group than up-regulated group. CONCLUSIONS: miR-219-2-3p is potentially involved in gastric cancer progression and metastasis by regulating ERK1/2-related signal pathways, which may provide a novel therapeutic strategy 8. Kinetics of the benzyl + O(3P) reaction: a quantum chemical/statistical reaction rate theory study. Science.gov (United States) da Silva, Gabriel; Bozzelli, Joseph W 2012-12-14 The resonance stabilized benzyl radical is an important intermediate in the combustion of aromatic hydrocarbons and in polycyclic aromatic hydrocarbon (PAH) formation in flames. Despite being a free radical, benzyl is relatively stable in thermal, oxidizing environments, and is predominantly removed through bimolecular reactions with open-shell species other than O(2). In this study the reaction of benzyl with ground-state atomic oxygen, O((3)P), is examined using quantum chemistry and statistical reaction rate theory. C(7)H(7)O energy surfaces are generated at the G3SX level, and include several novel pathways. Transition state theory is used to describe elementary reaction kinetics, with canonical variational transition state theory applied for barrierless O atom association with benzyl. Apparent rate constants and branching ratios to different product sets are obtained as a function of temperature and pressure from solving the time-dependent master equation, with RRKM theory for microcanonical k(E). These simulations indicate that the benzyl + O reaction predominantly forms the phenyl radical (C(6)H(5)) plus formaldehyde (HCHO), with lesser quantities of the C(7)H(6)O products benzaldehyde, ortho-quinone methide, and para-quinone methide (+H), along with minor amounts of the formyl radical (HCO) + benzene. Addition of O((3)P) to the methylene site in benzyl produces a highly vibrationally excited C(7)H(7)O* adduct, the benzoxyl radical, which can β-scission to benzaldehyde + H and phenyl + HCHO. In order to account for the experimental observation of benzene as the major reaction product, a roaming radical mechanism is proposed that converts the nascent products phenyl and HCHO to benzene + HCO. Oxygen atom addition at the ortho and para ring sites in benzyl, which has not been previously considered, is shown to lead to the quinone methides + H; these species are less-stable isomers of benzaldehyde that are proposed as important combustion intermediates, but 9. Facile Synthesis of Colored and Conducting CuSCN Composite Coated with CuS Nanoparticles Science.gov (United States) Premalal, E. V. A.; Kannangara, Yasun Y.; Ratnayake, S. P.; Nalin de Silva, K. M. 2017-08-01 Conductivity-tunable, different colored CuS nanoparticle-coated CuSCN composites were synthesized in a single pot using a mixture of copper sulfate and sodium thiosulfate in the presence of triethyl amine hydrothiocyanate (THT) at the ambient condition. When these reagents are mixed in 1:1:1 molar ratio, white-gray-colored CuSCN was produced. In the absence of THT, microsized dark blue-colored CuS particles were produced. However, when THT is present in the solution mixture by different amounts, colored conducting CuS nanoparticle-coated CuSCN composite was produced. CuS nanoparticles are not deposited on CuSCN soon after mixing these regents, but it takes nearly overnight to see the color change (CuS production) in the white CuSCN dispersed mixture. TEM analysis shows that composite consists of hexagonal CuS nanoparticles in the range of 3-10 nm in size. It is interesting to note that CuS-coated CuSCN possesses higher conductivity than neat CuS or CuSCN. Moreover, strong IR absorption was observed for CuS-coated CuSCN composite compared to neat CuS (absence of THT) or CuSCN. Lowest resistivity of 0.05 Ω cm was observed for annealed (250 °C) CuS-coated CuSCN particles (adding 10 ml of THT) under nitrogen atmosphere. Also, this simple method could be extended to be used in the synthesis of CuS-coated composites on the other nanomaterials such as metal oxides, polymers, and metal nanoparticles. 10. First-principles study on the elastic properties of B‧ and Q phase in Al-Mg-Si (-Cu) alloys Science.gov (United States) Pan, Rong-Kai; Ma, Li; Bian, Nan; Wang, Ming-Hui; Li, Peng-Bo; Tang, Bi-Yu; Peng, Li-Ming; Ding, Wen-Jiang 2013-01-01 First-principles calculations within the density functional theory have been carried out to study the structural, elastic and electronic properties of B‧ and Q phases in Al-Mg-Si (-Cu) alloys. The obtained lattice constant a is reduced while c is increased with the addition of Cu into B‧ phase Al3Mg9Si7. The lower formation enthalpy of Q phase Al3Cu2Mg9Si7 shows that the structural stability is improved after the addition of Cu into the B‧ phase. The calculated elastic constants Cij with the exception of C13 for Q phase are larger than for B‧ phase. In addition, the derived bulk, shear, Young's modulus and Debye temperature except Poisson's ratio are also significantly increased with Cu addition, indicating that Q phase has a favorable improvement of hardness. The elastic anisotropies of the two phases are discussed in detail using several criteria, showing that the anisotropy degree of B‧ phase is larger than of Q phase. The electronic structures show that the two phases possess a mixed bonding character of covalent and ionic, and Cu-Si bonding is beneficial in stabilizing the Q phase due to the hybridization of Cu 3d and Si 3p orbits. 11. Cu/Cu2O/CuO nanoparticles: Novel synthesis by exploding wire technique and extensive characterization Science.gov (United States) Sahai, Anshuman; Goswami, Navendu; Kaushik, S. D.; Tripathi, Shilpa 2016-12-01 In this article, we explore potential of Exploding Wire Technique (EWT) to synthesize the copper nanoparticles using the copper metal in a plate and wire geometry. Rietveld refinement of X-ray diffraction (XRD) pattern of prepared material indicates presence of mixed phases of copper (Cu) and copper oxide (Cu2O). Agglomerates of copper and copper oxide comprised of ∼20 nm average size nanoparticles observed through high resolution transmission electron microscope (HRTEM) and energy dispersive x-ray (EDX) spectroscopy. Micro-Raman (μR) and Fourier transform infrared (FTIR) spectroscopies of prepared nanoparticles reveal existence of additional minority CuO phase, not determined earlier through XRD and TEM analysis. μR investigations vividly reveal cubic Cu2O and monoclinic CuO phases based on the difference of space group symmetries. In good agreement with μRaman analysis, FTIR stretching modes corresponding to Cu2-O and Cu-O were also distinguished. Investigations of μR and FTIR vibrational modes are in accordance and affirm concurrence of CuO phases besides predominant Cu and Cu2O phase. Quantum confinement effects along with increase of band gaps for direct and indirect optical transitions of Cu/Cu2O/CuO nanoparticles are reflected through UV-vis (UV-vis) spectroscopy. Photoluminescence (PL) spectroscopy spots the electronic levels of each phase and optical transitions processes occurring therein. Iterative X-ray photoelectron spectroscopy (XPS) fitting of core level spectra of Cu (2p3/2) and O (1s), divulges presence of Cu2+ and Cu+ in the lattice with an interesting evidence of O deficiency in the lattice structure and surface adsorption. Magnetic analysis illustrates that the prepared nanomaterial demonstrates ferromagnetic behaviour at room temperature. 12. Synthsis of(3S,4S)-4-amino-3-hydroxy-6-methylheptanoic acid%（3 S,4 S）-4-氨基-3-羟基-6-甲基庚酸的合成 Institute of Scientific and Technical Information of China (English) 金瑛; 张天一; 刘帆 2015-01-01 (3S,4S)-4-(Dibenzylamino)-3-hydroxy-6-methyl-heptanoic acid was synthesized from L-Leucine by protection of amino and carboxyl group to give( S)-2-( dibenzylamino)-4-methylbenzyl valerate,which was subjected to nucleophilic substitution with acetontrile and stereoselective reduction with NaBH4 to afford(3S,4S)-4-(dibenzylamino)-3-hydroxy-6-methylheptanenitrile,fol-lowed by oxidation with hydrogen peroxide to give(3S,4S)-4-(dibenzylamino)-3-hydroxy-6-methylheptanoic acid. Finally,depro-tection of benzyl group in presence of Pd(OH)2/C-H2 to achieve(3S,4S)-4-amino-3-hydroxy-6-methylheptanoic acid in an overall yield of about 33. 6%.%价廉易得的L-亮氨酸先以苄基同时保护氨基及羧基得( S)-2-(二苄胺基)-4-甲基戊酸苄酯,进而在碱性条件下与乙腈发生亲核取代反应得( S)-4-(二苄胺基)-6-甲基-3-氧代庚腈,再经硼氢化钠选择性还原羰基得(3S,4S)-4-(二苄胺基)-3-羟基-6-甲基庚腈,用双氧水氧化得(3S,4S)-4-(二苄胺基)-3-羟基-6-甲基庚酸,最后在Pd(OH)2/C-H2作用下脱掉苄基得到(3S,4S)-4-氨基-3-羟基-6-甲基庚酸,即(3S,4S)-statine。整个合成路线总产率为33．6%。 13. Electrochemical deposition and modification of Cu/Co-Cu multilayer Energy Technology Data Exchange (ETDEWEB) Uhlemann, M.; Gebert, A.; Herrich, M.; Krause, A.; Cziraki, A.; Schultz, L 2003-09-30 Multilayers of Cu/Co-Cu were electrodeposited from a citrate electrolyte on quartz with Au and Si substrates with a Permalloy/Cu seedlayer by means of the potentiostatic double-pulse technique simultaneously controlled by microgravimetric measurements. The influence of a superimposed magnetic field and of annealing conditions on the microstructure has been studied. When an external magnetic field is parallel-orientated toward the electrode, an increase of the Cu deposition rate was observed due to magnetohydrodynamic effects. In contrast, the deposition rate of Co decreases. Microstructural investigations have shown that both a superimposed uniform magnetic field up to 600 mT and an inhomogeneous field of an SmCo permanent magnet change the microstructure and improve the GMR effect. X-ray diffraction measurements revealed that all grains are arranged in the <1 1 1> direction in contrast to layers formed without magnetic field with only 75% of the grains having a (1 1 1) texture. Isothermal heat treatment at different temperatures increases the GMR effect corresponding to an increase of grain size with preferred (1 1 1) orientation. 14. miR-625-3p regulates oxaliplatin resistance by targeting MAP2K6-p38 signalling in human colorectal adenocarcinoma cells DEFF Research Database (Denmark) Rasmussen, Mads Heilskov; Lyskjær, Iben; Jersie-Christensen, Rosa Rakownikow 2016-01-01 the signalling networks affected by miR-625-3p. We show that the p38 MAPK activator MAP2K6 is a direct target of miR-625-3p, and, accordingly, is downregulated in non-responder patients of oxaliplatin therapy. miR-625-3p-mediated resistance is reversed by anti-miR-625-3p treatment and ectopic expression of a mi......R-625-3p insensitive MAP2K6 variant. In addition, reduction of p38 signalling by using siRNAs, chemical inhibitors or expression of a dominant-negative MAP2K6 protein induces resistance to oxaliplatin. Transcriptome, proteome and phosphoproteome profiles confirm inactivation of MAP2K6-p38 signalling...... as one likely mechanism of oxaliplatin resistance. Our study shows that miR-625-3p induces oxaliplatin resistance by abrogating MAP2K6-p38-regulated apoptosis and cell cycle control networks, and corroborates the predictive power of miR-625-3p.... 15. A Feedback Regulatory Loop between G3P and Lipid Transfer Proteins DIR1 and AZI1 Mediates Azelaic-Acid-Induced Systemic Immunity Directory of Open Access Journals (Sweden) Keshun Yu 2013-04-01 Full Text Available Systemic acquired resistance (SAR, a highly desirable form of plant defense, provides broad-spectrum immunity against diverse pathogens. The recent identification of seemingly unrelated chemical inducers of SAR warrants an investigation of their mutual interrelationships. We show that SAR induced by the dicarboxylic acid azelaic acid (AA requires the phosphorylated sugar derivative glycerol-3-phosphate (G3P. Pathogen inoculation induced the release of free unsaturated fatty acids (FAs and thereby triggered AA accumulation, because these FAs serve as precursors for AA. AA accumulation in turn increased the levels of G3P, which is required for AA-conferred SAR. The lipid transfer proteins DIR1 and AZI1, both of which are required for G3P- and AA-induced SAR, were essential for G3P accumulation. Conversely, reduced G3P resulted in decreased AZI1 and DIR1 transcription. Our results demonstrate that an intricate feedback regulatory loop among G3P, DIR1, and AZI1 regulates SAR and that AA functions upstream of G3P in this pathway. 16. Emergence of a novel equine-like G3P[8] inter-genogroup reassortant rotavirus strain associated with gastroenteritis in Australian children. Science.gov (United States) Cowley, Daniel; Donato, Celeste M; Roczo-Farkas, Susie; Kirkwood, Carl D 2016-02-01 During 2013, a novel equine-like G3P[8] rotavirus emerged as the dominant strain in Australian children with severe rotavirus gastroenteritis. Full genome analysis demonstrated that the strain was an inter-genogroup reassortant, containing an equine-like G3 VP7, a P[8] VP4 and a genogroup 2 backbone I2-R2-C2-M2-A2-N2-T2-E2-H2. The genome constellation of the equine-like G3P[8] was distinct to Australian and global G3P[8] strains. Phylogenetic analysis demonstrated a genetic relationship to multiple gene segments of Japanese strains RVA/JPN/S13-30/2013/G3P[4] and RVA/Human-wt/JPN/HC12016/2012/G1P[8]. The Australian equine-like G3P[8] strain displayed a distinct VP7 antigenic profile when compared with the previously circulating Australian G3P[8] strains. Identification of similar genes in strains from several geographical regions suggested the equine-like G3P[8] strain was derived by multiple reassortment events between globally co-circulating strains from both human and animal sources. This study reinforces the dynamic nature of rotavirus strains and illustrates the potential for novel human/animal reassortant strains to emerge within the human population. 17. The miR-24-3p/p130Cas: a novel axis regulating the migration and invasion of cancer cells. Science.gov (United States) Kang, Hoin; Rho, Jun Gi; Kim, Chongtae; Tak, Hyosun; Lee, Heejin; Ji, Eunbyul; Ahn, Sojin; Shin, A-Ri; Cho, Hyun-Il; Huh, Yun Hyun; Song, Woo Keun; Kim, Wook; Lee, Eun Kyung 2017-03-24 MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression by suppressing translation or facilitating mRNA decay. Differential expression of miRNAs is involved in the pathogenesis of several diseases including cancer. Here, we investigated the role of-miR-24-3p as a downregulated miRNA in metastatic cancer. miR-24-3p was decreased in metastatic cancer and lower expression of miR-24-3p was related to poor survival of cancer patients. Consistently, ectopic expression of miR-24-3p suppressed the cell migration, invasion, and proliferation of MCF7, Hep3B, B16F10, SK-Hep1, and PC-3 cells by directly targeting p130Cas. Stable expression of p130Cas restored miR-24-3p-mediated inhibition of cell migration and invasion. These results suggest that miR-24-3p functions as a tumor suppressor and the miR-24-3p/p130Cas axis is a novel factor of cancer progression by regulating cell migration and invasion. 18. Dynamics of interfacial reactions between O({sup 3} P) atoms and long-chain liquid hydrocarbons Energy Technology Data Exchange (ETDEWEB) Allan, Mhairi [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); Bagot, Paul A J [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); Koehler, Sven P K [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); Reed, Stewart K [Department of Physics and Astronomy, University of Edinburgh, The King' s Buildings, Edinburgh EH9 3JZ (United Kingdom); Westacott, Robin E [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); Costen, Matthew L [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); McKendrick, Kenneth G [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom) 2007-09-15 Recent progress that has been made towards understanding the dynamics of collisions at the gas-liquid interface is summarized briefly. We describe in this context a promising new approach to the experimental study of gas-liquid interfacial reactions that we have introduced. This is based on laser-photolytic production of reactive gas-phase atoms above the liquid surface and laser-spectroscopic probing of the resulting nascent products. This technique is illustrated for reaction of O({sup 3}P) atoms at the surface of the long-chain liquid hydrocarbon squalane (2,6,10,15,19,23-hexamethyltetracosane). Laser-induced fluorescence detection of the nascent OH has revealed mechanistically diagnostic correlations between its internal and translational energy distributions. Vibrationally excited OH molecules are able to escape the surface. At least two contributions to the product rotational distributions are identified, confirming and extending previous hypotheses of the participation of both direct and trapping-desorption mechanisms. We speculate briefly on future experimental and theoretical developments that might be necessary to address the many currently unanswered mechanistic questions for this, and other, classes of gas-liquid interfacial reaction. 19. Electronic nonadiabatic effects in low temperature radical-radical reactions. I. C(3P) + OH(2Π). Science.gov (United States) Maergoiz, A I; Nikitin, E E; Troe, J 2014-07-28 The formation of collision complexes, as a first step towards reaction, in collisions between two open-electronic shell radicals is treated within an adiabatic channel approach. Adiabatic channel potentials are constructed on the basis of asymptotic electrostatic, induction, dispersion, and exchange interactions, accounting for spin-orbit coupling within the multitude of electronic states arising from the separated reactants. Suitable coupling schemes (such as rotational + electronic) are designed to secure maximum adiabaticity of the channels. The reaction between C((3)P) and OH((2)Π) is treated as a representative example. The results show that the low temperature association rate coefficients in general cannot be represented by results obtained with a single (generally the lowest) potential energy surface of the adduct, asymptotically reaching the lowest fine-structure states of the reactants, and a factor accounting for the thermal population of the latter states. Instead, the influence of non-Born-Oppenheimer couplings within the multitude of electronic states arising during the encounter markedly increases the capture rates. This effect extends up to temperatures of several hundred K. 20. Theoretical investigations of α,α,α-trifluoro-3, -p and o-nitrotoluene by means of density functional theory. Science.gov (United States) Yildirim, G; Senol, S D; Dogruer, M; Ozturk, O; Senol, A; Tasci, A T; Terzioglu, C 2012-01-01 This study reports the optimized molecular structures, vibrational frequencies including Infrared intensities and Raman activities, corresponding vibrational assignments, (1)H and (13)C NMR chemical shifts, the magnitudes of the JCH and JCC coupling constants, Ultraviolet-visible (UV-vis) spectra, thermodynamic properties and atomic charges of the title compounds, α,α,α-trifluoro-3, -p and o-nitrotoluene, in the ground state by means of the density functional theory (DFT) with the standard B3LYP/6-311++G(d,p) method and basis set combination for the first time. Theoretical vibrational spectra were interpreted by normal coordinate analysis based on scaled density functional force field. The results show that the vibrational frequencies and chemical shifts calculated were obtained to be in good agreement with the experimental data. Based on the comparison between experimental results and theoretical data, the calculation level chosen is powerful approach for understanding the identification of all the molecules studied. In addition, not only were frontier molecular orbitals (HOMO and LUMO), molecular electrostatic potential (MEP) and electrostatic potential (ESP) simulated but also the dipole moment, softness, electronegativity, chemical hardness, electrophilicity index, transition state and energy band gap values were predicted. According to the investigations, all compounds were found to be useful to bond metallically and interact intermolecularly; however, the thermodynamic properties confirm that the α,α,α-trifluoro-p-nitrotoluene was more reactive and more polar than the others. 1. Semirigid vibrating rotor target calculation for reaction O(3p)+CH4 →CH3+OH Institute of Scientific and Technical Information of China (English) LIU; Xinguo; BAI; Lihua; ZHANG; Qinggang 2004-01-01 The time-dependent quantum dynamics calculation for reaction O(3p)+CH4→ CH3+OH is made, using of the semirigid vibrating rotor target (SVRT) model and the time-dependent wave packet (TDWP) method. The corresponding reaction probabilities of different initial states are provided. From the calculation of initial rovibrational state j= 0,v= 0, 1, we can see that the excitation of the H-CH3 stretching vibration gives significant enhancement of reaction probability and the reaction threshold decreases dramatically with the enhancement of the vibrating excitation, which indicates that the vibrating energy of reagent molecules contributes a lot to the molecular collision. As for the calculation of reaction probability of state v= 0, j= 0,1,2,3, the results show that the reaction probability rises significantly with the enhancement of rotational quantum number j while the reaction threshold has no changes. The spatial steric effect of the title reaction is studied and analyzed too after the calculation of reaction probability of states j= 5, k= 0-2, n= 0 and j=5, k=2, n=0-2 is made. 2. Electrical Investigation of Nanostructured Fe2O3/p-Si Heterojunction Diode Fabricated Using the Sol-Gel Technique Science.gov (United States) Mansour, Shehab A.; Ibrahim, Mervat M. 2017-07-01 Iron oxide (α-Fe2O3) nanocrystals have been synthesized via the sol-gel technique. The structural and morphological features of these nanocrystals were studied using x-ray diffraction, Fourier transform-infrared spectroscopy and transmission electron microscopy. Colloidal solution of synthesized α-Fe2O3 (hematite) was spin-coated onto a single-crystal p-type silicon (p-Si) wafer to fabricate a heterojunction diode with Mansourconfiguration Ag/Fe2O3/p-Si/Al. This diode was electrically characterized at room temperature using current-voltage (I-V) characteristics in the voltage range from -9 V to +9 V. The fabricated diode showed a good rectification behavior with a rectification factor 1.115 × 102 at 6 V. The junction parameters such as ideality factor, barrier height, series resistance and shunt resistance are determined using conventional I-V characteristics. For low forward voltage, the conduction mechanism is dominated by the defect-assisted tunneling process with conventional electron-hole recombination. However, at higher voltage, I-V ohmic and space charge-limited current conduction was became less effective with the contribution of the trapped-charge-limited current at the highest voltage range. 3. Theoretical investigations of α,α,α-trifluoro-3, -p and o-nitrotoluene by means of density functional theory Science.gov (United States) Yildirim, G.; Senol, S. D.; Dogruer, M.; Ozturk, O.; Senol, A.; Tasci, A. T.; Terzioglu, C. 2012-01-01 This study reports the optimized molecular structures, vibrational frequencies including Infrared intensities and Raman activities, corresponding vibrational assignments, 1H and 13C NMR chemical shifts, the magnitudes of the JCH and JCC coupling constants, Ultraviolet-visible (UV-vis) spectra, thermodynamic properties and atomic charges of the title compounds, α,α,α-trifluoro-3, -p and o-nitrotoluene, in the ground state by means of the density functional theory (DFT) with the standard B3LYP/6-311++G(d,p) method and basis set combination for the first time. Theoretical vibrational spectra were interpreted by normal coordinate analysis based on scaled density functional force field. The results show that the vibrational frequencies and chemical shifts calculated were obtained to be in good agreement with the experimental data. Based on the comparison between experimental results and theoretical data, the calculation level chosen is powerful approach for understanding the identification of all the molecules studied. In addition, not only were frontier molecular orbitals (HOMO and LUMO), molecular electrostatic potential (MEP) and electrostatic potential (ESP) simulated but also the dipole moment, softness, electronegativity, chemical hardness, electrophilicity index, transition state and energy band gap values were predicted. According to the investigations, all compounds were found to be useful to bond metallically and interact intermolecularly; however, the thermodynamic properties confirm that the α,α,α-trifluoro-p-nitrotoluene was more reactive and more polar than the others. 4. Performance Parameters Analysis of an XD3P Peugeot Engine Using Artificial Neural Networks (ANN) Concept in MATLAB Science.gov (United States) Rangaswamy, T.; Vidhyashankar, S.; Madhusudan, M.; Bharath Shekar, H. R. 2015-04-01 The current trends of engineering follow the basic rule of innovation in mechanical engineering aspects. For the engineers to be efficient, problem solving aspects need to be viewed in a multidimensional perspective. One such methodology implemented is the fusion of technologies from other disciplines in order to solve the problems. This paper mainly deals with the application of Neural Networks in order to analyze the performance parameters of an XD3P Peugeot engine (used in Ministry of Defence). The basic propaganda of the work is divided into two main working stages. In the former stage, experimentation of an IC engine is carried out in order to obtain the primary data. In the latter stage the primary database formed is used to design and implement a predictive neural network in order to analyze the output parameters variation with respect to each other. A mathematical governing equation for the neural network is obtained. The obtained polynomial equation describes the characteristic behavior of the built neural network system. Finally, a comparative study of the results is carried out. 5. MAP3S Precipitation Chemistry Network: second periodic summary report, July 1977--June 1978 Energy Technology Data Exchange (ETDEWEB) 1979-01-01 The MAP3S Precipitation Chemistry Network consists of eight sites located in the northeastern United States. Precipitation event samples are collected by cooperating site operators, using specially developed sampling equipment. The concentration data collected over the period July 1, 1977 to July 1, 1978, are listed as a summary of the data reported monthly throughout the year. Samples were chemically analyzed at a central laboratory for 13 pollutant species - pH, conductivity, SO/sub 2/, SO/sub 4//sup =/, NH/sub 4//sup +/, NO/sub 2//sup -/, NO/sub 3//sup -/, Cl/sup -/, PO/sub 4//sup 3 -/, Na/sup +/, K/sup +/, Ca/sup + +/, and Mg/sup + +/ - using ion chromatography, automated wet chemistry, atomic absorption spectrophotometry, and electrode techniques. Second-year developments included: the installation of refrigeration equipment in all Battelle collectors; the initiation of an externally administered quality control program; and use of ion chromatography for cation as well as anion species. Supplementary research efforts included a special collector comparison study at the Pennsylvania State site and further analysis of sulfite versus sulfate deposition. 6. MAP3S precipitation chemistry network. Third periodic summary report, July 1978-December 1979 Energy Technology Data Exchange (ETDEWEB) 1980-05-01 The MAP3S Precipitation Chemistry Network consists of eight collection sites in the northeastern United States. Precipitation event samples are collected by cooperating site operators, using specially developed sampling equipment. In this, the third periodic summary report, are listed field and concentration data for the period July 1, 1978 to December 31, 1979. Over three years' samples have been collected at most of the sites, which went into operation between September 1976 and October 1978. Samples are chemically analyzed at a central laboratory for 13 pollutant species. Weekly samples in addition to event samples were collected over a 1 1/2 year period at three sites. Analysis of one year's results indicates that there is little difference between the concentrations collected by the two methods in terms of seasonal precipitation-weighted means for all species except dissolved SO/sub 2/. Event samples tend to average about 25% higher in SO/sub 2/ than weekly samples. 7. Cubic H3S around 200 GPa: An atomic hydrogen superconductor stabilized by sulfur Science.gov (United States) Papaconstantopoulos, D. A.; Klein, B. M.; Mehl, M. J.; Pickett, W. E. 2015-05-01 The multiple scattering-based theory of Gaspari and Gyorffy for the electron-ion matrix element in close packed metals is applied to I m 3 ¯m H3S , which has been predicted by Duan et al. and Bernstein et al. to be the stable phase at this stoichiometry around 190 GPa, thus is the leading candidate to be the phase observed to superconduct at 190 K by Drozdov, Eremets, and Troyan. The nearly perfect separation of vibrational modes into those of S and of H character provides a simplification that enables identification of contributions of the two atoms separately. The picture that arises is basically that of superconducting atomic H stabilized by strong covalent mixing with S p and d character. The reported isotope shift is much larger than the theoretical one, suggesting there is large anharmonicity in the H vibrations. Given the relative unimportance of sulfur, hydrides of lighter atoms at similarly high pressures may also lead to high temperature superconductivity. 8. Defect characterization of Ga$_4$Se$_3$S layered single crystals by thermoluminescence Indian Academy of Sciences (India) Isik M; Delice S; Gasanly N 2016-04-01 Trapping centres in undoped Ga$_4$Se$_3S single crystals grown by Bridgman method were characterized for the first time by thermoluminescence (TL) measurements carried out in the low temperature range of 15−300 K. After illuminating the sample with blue light (∼470 nm) at 15 K, TL glow curve exhibited one peak around 74 K when measured with a heating rate of 0.4 K/s.The results of the various analysis methods were in good agreement about the presence of one trapping centre with an activation energy of 27 meV. Analysis of curve fitting method indicated that mixed order of kinetics dominates the trapping process. Heating rate dependence and distribution of the traps associated with the observed TL peak were also studied. The shift of peak maximum temperature from 74 to 113 K with increasing rate from 0.4 to 1.2 K/s was revealed. Distribution of traps was investigated using an experimental technique based on cleaning the centres giving emission at lower temperatures. Activation energies of the levels were observed to be increasing from 27 to 40 meV by rising the stopping temperature from 15 to 36 K. 9. MAP3S precipitation chemistry network: first periodic summary report, (September 1976--June 1977) Energy Technology Data Exchange (ETDEWEB) 1977-10-01 The MAP3S Precipitation Chemistry Network consists of four sites (to be expanded to 8 to 12) located in a north-south line from northern New York State to northwestern Virginia. Precipitation event samples are collected by cooperating site operators, using specially developed sampling equipment. The concentration data collected over the period September 22, 1976 (first collected sample) to July 1, 1977, are listed as a summary of the data reported monthly throughout the year. Samples were chemically analyzed at a central laboratory for 11 pollutant species, pH, conductivity, SO/sub 2/, SO/sup 2 -/, NH/sub 4//sup +/, NO/sub 2//sup -/, NO/sub 3//sup -/, Cl/sup -/, PO/sub 4//sup 3 -/, Na/sup +/, and K/sup +/, using ion chromatography, automated wet chemistry, atomic absorption spectrophotometry, and other standard techniques. The new precipitation collector equipment was evaluated by comparing the concentrations measured with those from a proven type of collector, operated simultaneously at all four sites. 10. [Application of 3S techniques in ecological landscape planning of Harbin suburb]. Science.gov (United States) Fan, Wenyi; Gong, Wenfeng; Liu, Dandan; Zhou, Hongze; Zhu, Ning 2005-12-01 With the image data (SPOT), soil utilization map (1:50000) and other related materials of Harbin, and under the support of GIS, RS and GPS techniques, this paper obtained the landscape pattern of Harbin suburb and the Digital Elevation Model (DEM) of Harbin. Indices including mean patch area, landscape dominance, mean slope, mean altitude, and fragmentation degree were selected and synthetically analyzed, with the ecological landscape planning made by DEM model. The results showed that 3S techniques could help to decide typical landscape types. The landscape type database was established, and the landscape type thematic map was generated, with land use status and landscape distribution, physiognomy, and land use types combined. The ecological landscape planning was described in large scale with the image data and DEM combined, and the landscape structure of Harbin suburb was reflected directly with the ecological landscape planning and DEM combined, which improved the ecological function in this region, and provided scientific bases to the healthy development in urban-rural integration area. 11. Radiative Bottomonium Spectroscopy at the Y(2, 3S) Resonances at BaBar Energy Technology Data Exchange (ETDEWEB) Lewis, Peter M. [Stanford Univ., CA (United States) 2013-08-01 The compact bound state consisting of a bottom and anti-bottom quark pair interacting via the strong nuclear force is called “bottomonium.” A wealth of long-lived bottomonium states can be both experimentally produced and theoretically described, providing a unique tool to probe calculation techniques with experiment. Bottomonia with total angular momentum J = 1 and orbital angular momentum L = 0 at a variety of radial excitations n – called Υ(nS) – can be produced at electron-positron colliders. The BABAR experiment, located at the interaction point of such a collider (the PEP-II storage ring), has observed 122 million Υ(3S) and 100 million Υ(2S) decays. Some of these involve a transition to the bottomonium state χbJ (nP) (L = 1 and J = (0, 1, 2)), emitting a photon, with subsequent transition to a lower Υ(nS), also emitting a photon. The final Υ(nS) can be identified through a decay to two muons. The dependence of the branching fractions and photon energies in this process on the spin state of the intermediate χbJ (nP) is a key test of phenomenological models. To this end, this dissertation contains a nearly comprehensive study of these transitions with an emphasis on experimentally optimal discrimination between various models. This focus spurs innovative techniques that complement a large array of physics results, both presented in detail herein. 12. 多通道反应O(3P)+CH2F的理论研究%Vibrational Mode Analysis for the Multi-channel Reactions of O(3P) with CH2F Institute of Scientific and Technical Information of China (English) 曹小龙; 郭丽 2004-01-01 用密度泛函理论研究了氧原子与氟代甲基自由基的反应.反应中出现的所有物种的平衡构型用B3LYP方法在6-311++G(2 d,2p)基组水平上进行了优化,同时对各物种进行了频率分析;在同一理论水平上计算了各反应通道的势能面变化,分析了反应物、中间体、过渡态、产物的振动模式随反应途径的变化关系,阐明了该多通道反应的反应机理.%All species involved in the multi-channel reaction of an oxygen atom with a fluorinated methyl radical have been investigated using density functional theory (DFT) . The geometries of the reactants, intermediates, transition states and products are optimized at the B3LYP/6-311 + + G(2 d, 2p) level. The potential energy surface for this reaction is calculated at the same level of theory. Various possible production channels involved in the reaction of O(3 P) +CH2F are examined. The vibrational mode analysis is used to elucidate the relationship between the transition states, intermediates and the products. The extensive investigation shows that the reaction mechanism is reliable. 13. MicroRNA-143-3p inhibits hyperplastic scar formation by targeting connective tissue growth factor CTGF/CCN2 via the Akt/mTOR pathway. Science.gov (United States) Mu, Shengzhi; Kang, Bei; Zeng, Weihui; Sun, Yaowen; Yang, Fan 2016-05-01 Post-traumatic hypertrophic scar (HS) is a fibrotic disease with excessive extracellular matrix (ECM) production, which is a response to tissue injury by fibroblasts. Although emerging evidence has indicated that miRNA contributes to hypertrophic scarring, the role of miRNA in HS formation remains unclear. In this study, we found that miR-143-3p was markedly downregulated in HS tissues and fibroblasts (HSFs) using qRT-PCR. The expression of connective tissue growth factor (CTGF/CCN2) was upregulated both in HS tissues and HSFs, which is proposed to play a key role in ECM deposition in HS. The protein expression of collagen I (Col I), collagen III (Col III), and α-smooth muscle actin (α-SMA) was obviously inhibited after treatment with miR-143-3p in HSFs. The CCK-8 assay showed that miR-143-3p transfection reduced the proliferation ability of HSFs, and flow cytometry showed that either early or late apoptosis of HSFs was upregulated by miR-143-3p. In addition, the activity of caspase 3 and caspase 9 was increased after miR-143-3p transfection. On the contrary, the miR-143-3p inhibitor was demonstrated to increase cell proliferation and inhibit apoptosis of HSFs. Moreover, miR-143-3p targeted the 3'-UTR of CTGF and caused a significant decrease of CTGF. Western blot demonstrated that Akt/mTOR phosphorylation and the expression of CTGF, Col I, Col III, and α-SMA were inhibited by miR-143-3p, but increased by CTGF overexpression. In conclusion, we found that miR-143-3p inhibits hypertrophic scarring by regulating the proliferation and apoptosis of human HSFs, inhibiting ECM production-associated protein expression by targeting CTGF, and restraining the Akt/mTOR pathway. 14. MicroRNA-125a-3p expression in abdominal adipose tissues is associated with insulin signalling gene expressions in morbid obesity: observations in Taiwanese. Science.gov (United States) Yeh, Chiu-Li; Cheng, I-Chi; Hou, Yu-Chen; Wang, Weu; Yeh, Sung-Ling 2014-01-01 Micro (mi) RNAs have been found to play an important role in the regulation of adipogenesis and insulin sensitivity. However, associations between miRNA and insulin signalling-related gene expressions in abdominal adipose tissues in obese subjects remain unclear. We used a microarray platform to screen miRNA expressions in abdominal adipose tissues between genders in severely obese subjects and found that the top-ranking miRNA in abdominal omental adipose tissues was miRNA-125a-3p. MicroR-125a-3p and insulin signalling-related gene expressions in abdominal omental adipose tissues of all subjects (11 men and 10 women) were subsequently quantified by a real-time PCR. Also, associations of miR-125a-3p with insulin signalling-related gene expression and biochemical markers in obese subjects were analyzed by a linear regression analysis. miR-125a-3p expressed by abdominal omental adipose tissues was much higher in obese men than women. No gender difference was observed in abdominal subcutaneous adipose tissues. Concomitant with high miR-125a-3p, c-Jun N-terminal kinase gene expression was also higher, whereas insulin receptor was lower in men than women. There were negative associations of miR-125a-3p with the insulin receptor and phosphatidylinositol 3-kinase expressions. Fasting plasma glucose and cholesterol levels were positively associated with miR- 125a-3p expression. These associations were obvious in obese men but not women. Our results support the involvement of miR-125a-3p in regulating the insulin signalling pathway and imply that increased miR- 125a-3p expression in omental adipose tissues may be a characteristic feature of insulin resistance in obese men. 15. MiR-338-3p inhibits hepatocarcinoma cells and sensitizes these cells to sorafenib by targeting hypoxia-induced factor 1α. Directory of Open Access Journals (Sweden) Haitao Xu Full Text Available Hypoxia is a common feature of solid tumors and an important contributor to anti-tumor drug resistance. Hypoxia inducible factor-1 (HIF-1 is one of the key mediators of the hypoxia signaling pathway, and was recently proven to be required for sorafenib resistance in hepatocarcinoma (HCC. MicroRNAs have emerged as important posttranslational regulators in HCC. It was reported that miR-338-3p levels are associated with clinical aggressiveness of HCC. However, the roles of miR-338-3p in HCC disease and resistance to its therapeutic drugs are unknown. In this study, we found that miR-338-3p was frequently down-regulated in 14 HCC clinical samples and five cell lines. Overexpression of miR-338-3p inhibited HIF-1α 3'-UTR luciferase activity and HIF-1α protein levels in HepG2, SMMC-7721, and Huh7 cells. miR-338-3p significantly reduced cell viability and induced cell apoptosis of HCC cells. Additionally, HIF-1α overexpression rescued and HIF-1α knock-down abrogated the anti-HCC activity of miR-338-3p. Furthermore, miR-338-3p sensitized HCC cells to sorafenib in vitro and in a HCC subcutaneous nude mice tumor model by inhibiting HIF-1α. Collectively, miR-338-3p inhibits HCC tumor growth and sensitizes HCC cells to sorafenib by down-regulating HIF-1α. Our data indicate that miR-338-3p could be a potential candidate for HCC therapeutics. 16. Up-regulation of miR-95-3p in hepatocellular carcinoma promotes tumorigenesis by targeting p21 expression Science.gov (United States) Ye, Jian; Yao, Yufeng; Song, Qixue; Li, Sisi; Hu, Zhenkun; Yu, Yubing; Hu, Changqing; Da, Xingwen; Li, Hui; Chen, Qiuyun; Wang, Qing K. 2016-01-01 Hepatocellular carcinoma (HCC) is one of the most common malignant cancers. To elucidate new regulatory mechanisms for heptocarcinogenesis, we investigated the regulation of p21, a cyclin-dependent kinase (CDK) inhibitor encoded by CDKN1A, in HCC. The expression level of p21 is decreased with the progression of HCC. Luciferase assays with a luciferase-p21-3′ UTR reporter and its serial deletions identified a 15-bp repressor element at the 3′-UTR of CDKN1A, which contains a binding site for miR-95-3p. Mutation of the binding site eliminated the regulatory effect of miR-95-3p on p21 expression. Posttranscriptional regulation of p21 expression by miR-95-3p is mainly on the protein level (suppression of translation). Overexpression of miR-95-3p in two different HCC cell lines, HepG2 and SMMC7721, significantly promoted cell proliferation, cell cycle progression and cell migration, whereas a miR-95-3p specific inhibitor decreased cell proliferation, cell cycle progression and cell migration. The effects of miR-95-3p on cellular functions were rescued by overexpression of p21. Overexpression of miR-95-3p promoted cell proliferation and tumor growth in HCC xenograft mouse models. Expression of miR-95-3p was significantly higher in HCC samples than in adjacent non-cancerous samples. These results demonstrate that miR-95-3p is a potential new marker for HCC and regulates hepatocarcinogenesis by directly targeting CDKN1A/p21 expression. PMID:27698442 17. miRNA-556-3p promotes tumorigenesis and metastasis by negatively regulating DAB2IP expression in human bladder cancer Institute of Scientific and Technical Information of China (English) CHEN Feng; SUN Ping; LI Mingqiu; LIU Yueguang; FENG Yukuan; FENG Kejian 2015-01-01 Objective:MicroRNAs ( miRNAs) function as key regulator of gene expression and their dereg-ulation play critical roles in tumorigenesis and metastasis of various cancers. The purpose of this study is to identify miRNAs targeting DAB2IP and to determine their expression and function in bladder cancer (BC). Methods and Results:We first predicted candidate miRNAs targeting Disabled homolog 2- interaction protein ( DAB2IP) and then determine their expression and biological function in BC. We showed that miRNA-556-3p directly regulated DAB2 IP expression by binding to DAB2 IP 3 '-UTR and endogenous miRNA-556-3 p expression was significantly up-regulated in clinical samples of BC patients and BC cell lines in comparison to the controls. Conversely, simul-taneous DAB2IP expression in BC tissues and BC cell lines was remarkably down-regulated. Gain or loss function showed that enhanced miRNA-556-3p expression by Lv-miRNA-556-3p transfection promoted proliferation,in-vasion, migration, and colony formation of BC cells, whereas repressed miRNA-556-3p expression by Lv-sh-miRNA-556-3p transfection resulted inan opposite results. Importantly,restored DAB2IP expression by "rescue"assay could attenuate the promotion effect induced by miRNA-556-3p. Further investigation verified that overex-pressed miRNA-556-3p in BC cells not only decreased DAB2IP expression, but also dramatically increased Ras-and pERK1/2 protein expression. In conclusion, our results suggested that DAB2IP was a direct target of miRNA-556-3p, and endogenous miRNA-556-3p expression was reversely correlated with simultaneous DAB2IP expres-sion in BC tissues and cells. Conclusions: MiRNA-556-3p, as a tumor promoter, functioned in tumorigenesis and metastasis of BC via targeting DAB2IP. Moreover, miRNA-556-3p mediated DAB2IP suppression played an oncogenic role by activation of Ras-ERK pathway partially. 18. Properties of the ground^3$F$_2$state and the excited$^3$P$_0$state of atomic thorium in cold collisions with$^3$He CERN Document Server Au, Yat Shan; Ketterle, Wolfgang; Doyle, John M 2013-01-01 We measure inelastic collisional cross sections for the ground$^3$F$_2$state and the excited$^3$P$_0$state of atomic thorium in cold collisions with$^3$He. We determine for Th ($^3$F$_2$) at 800 mK the ratio$\\gamma \\approx 500$of the momentum-transfer to Zeeman relaxation cross sections for collisions with$^3$He. For Th ($^3$P$_0$), we study electronic inelastic processes and find no quenching even after$10^6$collisions. We also determine the radiative lifetime of Th ($^3$P$_0$) to be$\\tau > 130\$ ms. This great stability of the metastable state opens up the possibility for further study, including trapping.

19. Fe3S4 and Fe3O4 magnetic nanocrystals: magneto-optical and Mössbauer spectroscopy study

Science.gov (United States)

Lin, C.-R.; Tseng, Y.-T.; Ovchinnikov, S. G.; Ivantsov, R. D.; Edelman, I. S.; Fedorov, A. S.; Kuzubov, A. A.; Fedorov, D. A.; Starchikov, S. S.; Lyubutin, I. S.

2014-04-01

Iron oxide magnetite (Fe3O4) should be a reasonable analog for conception and understanding of the magnetic properties of iron sulfide greigite (Fe3S4)—one of the most required magnetic materials having numerous applications but being far from the complete understanding now. We present here a comparative study of the Mössbauer effect (ME) and the magnetic circular dichroism (MCD) spectroscopy of Fe3O4 and Fe3S4 nanoparticles. The ME spectrum parameters of Fe3S4 are shown to distinguish strongly from that of Fe3O4, and the MCD spectrum shapes are shown to be absolutely different for two compounds. To clarify the origin of the Fe3S4 MCD spectrum we have performed ab initio band structure calculations and identified the MCD spectrum features with the transitions between calculated energy states.

20. CuI Thiolate Reactivity with Dioxygen: The Formation of CuII Sulfinate and CuII Sulfonate Species via a CuII Thiolate Intermediate

NARCIS (Netherlands)

Ording-Wenker, E.C.M.; Siegler, M.A.M.; Lutz, M.; Bouwman, E.

2013-01-01

CuI(Py2NS) (1) is formed by addition of CuI to a solution of the pyridyl-thiol ligand N-(2-mercaptopropyl)- N,N-bis(2-pyridylmethyl)amine (Py2NSH). Oxidation of complex 1 by air leads to the formation of CuII sulfinate and CuII sulfonate complexes, providing a model for the oxidative degeneration of

1. Punicalagin Green Functionalized Cu/Cu2O/ZnO/CuO Nanocomposite for Potential Electrochemical Transducer and Catalyst

Science.gov (United States)

Fuku, X.; Kaviyarasu, K.; Matinise, N.; Maaza, M.

2016-09-01

A novel ternary Punica granatum L-Cu/Cu2O/CuO/ZnO nanocomposite was successfully synthesised via green route. In this work, we demonstrate that the green synthesis of metal oxides is more viable and facile compare to other methods, i.e., physical and chemical routes while presenting a potential electrode for energy applications. The prepared nanocomposite was characterised by both microscopic and spectroscopic techniques. High-resolution scanning electron microscopy (HRSEM) and X-ray diffraction (XRD) techniques revealed different transitional phases with an average nanocrystallite size of 29-20 mm. It was observed that the nanocomposites changed from amorphous-slightly crystalline Cu/Cu2O to polycrystalline Cu/Cu2O/CuO/ZnO at different calcination temperatures (room temperature-RT- 600 °C). The Cu/Cu2O/ZnO/CuO metal oxides proved to be highly crystalline and showed irregularly distributed particles with different sizes. Meanwhile, Fourier transform infrared (FTIR) spectroscopy confirmed the purity while together with ultraviolet-visible (UV-Vis) spectroscopy proved the proposed mechanism of the synthesised nanocomposite. UV-Vis showed improved catalytic activity of the prepared metal oxides, evident by narrow band gap energy. The redox and electrochemical properties of the prepared nanocomposite were achieved by cyclic voltammetry (CV), electrochemical impedance (EIS) and galvanostatic charge-discharge (GCD). The maximum specific capacitance ( C s) was calculated to be 241 F g-1 at 50 mV s-1 for Cu/Cu2O/CuO/ZnO nanoplatelets structured electrode. Moreover, all the CuO nanostructures reveal better power performance, excellent rate as well as long term cycling stability. Such a study will encourages a new design for a wide spectrum of materials for smart electronic device applications.

2. Compound Cuing in Free Recall

Science.gov (United States)

Lohnas, Lynn J.; Kahana, Michael J.

2014-01-01

According to the retrieved context theory of episodic memory, the cue for recall of an item is a weighted sum of recently activated cognitive states, including previously recalled and studied items as well as their associations. We show that this theory predicts there should be compound cuing in free recall. Specifically, the temporal contiguity…

3. Barrier/Cu contact resistivity

Energy Technology Data Exchange (ETDEWEB)

Reid, J.S.; Nicolet, M.A. [California Inst. of Tech., Pasadena, CA (United States); Angyal, M.S.; Lilienfeld, D.; Shacham-Diamand, Y. [Cornell Univ., Ithaca, NY (United States); Smith, P.M. [Sandia National Labs., Albuquerque, NM (United States)

1995-10-17

The specific contact resistivity of Cu with ({alpha} + {beta})-Ta, TiN, {alpha}-W, and amorphous-Ta{sub 36}Si{sub 14}N{sub 50} barrier films is measured using a novel four-point-probe approach. Geometrically, the test structures consist of colinear sets of W-plugs to act as current and voltage probes that contact the bottom of a planar Cu/barrier/Cu stack. Underlying Al interconnects link the plugs to the current source and voltmeter. The center-to-center distance of the probes ranges from 3 to 200 {micro}m. Using a relation developed by Vu et al., a contact resistivity of roughly 7 {times} 10{sup {minus}9} {Omega} cm{sup 2} is obtained for all tested barrier/Cu combinations. By reflective-mode small-angle X-ray scattering, the similarity in contact resistivity among the barrier films may be related to interfacial impurities absorbed from the deposition process.

4. Synthesis and Characterization of Indium Compounds with Phosphinothiol Ligands. The Crystal and Molecular Structures of [In{2-(Ph(2)P)C(6)H(4)S}(3)], [In{2-(Ph(2)P)-6-(Me(3)Si)C(6)H(3)S}(2){2-(Ph(2)PO)-6-(Me(3)Si)C(6)H(3)S}], and [NMe(4)][In{PhP(C(6)H(4)S-2)(2)}(2)].CH(3)CN.

Science.gov (United States)

Pérez-Lourido, Paulo; Romero, Jaime; García-Vázquez, José Arturo; Sousa, Antonio; Maresca, Kevin; Zubieta, Jon

1999-03-22

The electrochemical oxidation of anodic indium metal in an acetonitrile solution of phosphinothiol ligands affords [In{2-(Ph(2)P)C(6)H(4)S}(3)] (1), [In{2-(Ph(2)P)-6-(Me(3)Si)C(6)H(3)S)}(2){2-(Ph(2)PO)-6-(Me(3)Si)C(6)H(3)S}] (2), [In{2-(Ph(2)PO)-6-(Me(3)Si)C(6)H(3)S}(3)] (3), and [NMe(4)][In{PhP(C(6)H(4)S-2)(2)}(2)].CH(3)CN (4) complexes exhibiting distorted six-coordinate geometries based on {InS(3)P(3)}, {InS(3)P(2)O}, and {InS(4)P(2)} cores, respectively. In all cases, the In-P bond distances are anomalously long, presumably as a consequence of steric crowding. The anion of 4 provides an unusual example of an In(III)-thiolate coordination complex ion. Crystal data: 1, C(54)H(42)InP(3)S(3), monoclinic, P2(1)/c, a = 16.6579(1) Å, b = 12.6628(2) Å, c = 22.5520(3) Å, beta = 96.42(1) degrees, V = 4727.15(1) Å(3), Z = 4, 6176 reflections, R = 0.0579; 2, C(63)H(66)InOP(3)S(3)Si(3), monoclinic, P2(1)/c, a = 11.53090(10) Å, b = 26.2505(3) Å, c = 20.4206(2) Å, beta = 94.0870(10) degrees, V = 6165.43(11) Å(3), Z = 4, 10 699 reflections, R = 0.0621; 4, C(42)H(41)InN(2)P(2)S(4), monoclinic, P2(1), a = 13.0052(2) Å, b = 11.2240(2) Å, c = 14.3070(3) Å, beta = 93.190(1) degrees, V = 2085.16(7) Å(3), Z = 2, 6651 reflections, R = 0.0352.

5. Investigation of agglomerated Cu seed on Cu oxidation after chemical mechanical planarization

Energy Technology Data Exchange (ETDEWEB)

Lin, Jeng-Yu, E-mail: jylin@ttu.edu.tw [Department of Chemical Engineering, Tatung University, Taipei 104, Taiwan (China); Chou, Shu-Wei [Department of Chemical Engineering, Tatung University, Taipei 104, Taiwan (China); Cheng, Min-Yuan [TSMC, No. 8, Li-Hsin Rd. 6, Science-Based Industrial Park, Hsinchu 300-77, Taiwan (China)

2010-11-01

After chemical mechanical planarization (CMP), the reason which caused the formation of Cu-oxide defects at the interface between Cu deposit and TaN barrier layer has been studied. The experimental results of atomic force microscopy, secondary ion mass spectroscopy, X-ray diffraction demonstrated that the agglomeration phenomenon was found on Cu seed in the thickness of only 10 nm, thus resulting in the electrodeposited Cu film with more abundant C impurities at Cu/TaN interface and lower (1 1 1)/(2 0 0) ratio compared to the thick one (30 nm). Therefore it caused the Cu deposit with poor corrosion resistance and then the Cu-oxide defects were easily formed after CMP. As a result, the correlation between Cu-oxide defects at the Cu/TaN interface and the agglomeration on Cu seed layer was proposed herein.

6. MARTENSITIC TRANSFORMATION AND THERMAL STABILITY IN Cu-Al-Co AND Cu-Al-Zr ALLOYS

Institute of Scientific and Technical Information of China (English)

Y. Q. Ma; C.B. Jiang; H.B. Xu

2003-01-01

Cu-Al-Co and Cu-Al-Zr alloys were explored with Co or Zr additions in Cu-Al alloys for high temperature shape memory alloys. Samples were quenched after homogenized at 850℃ for 48h. It was found that both Cu-Al-Co and Cu-Al-Zr show AlCu3 martensitic phase at room temperature and exhibit martensitic transformation temperatures higher than 200℃, showing the potentials for developing as high temperature shape memory alloys. Thermal cycles were performed by DSC instrument on both Cu-Al-Co and Cu-Al-Zr alloys. The results show that Cu-Al-Co loses its martensitic transformation after five thermal cycles, and Cu-Al-Zr exhibits no martensitic transformation in the second thermal cycle.

7. Ionic Conductivities of Molten CuI and AgI-CuI Mixtures

Science.gov (United States)

Tahara, Shuta; Shimakura, Hironori; Ohno, Satoru; Fukami, Takanori

2017-08-01

Ionic conductivities σ for molten CuI and AgI-CuI mixtures were measured in the temperature ranges of approximately 580-800 and 500-850 °C, respectively. The value of σ for molten CuI in the range is smaller than that for molten CuBr and CuCl. σ for molten AgI-CuI mixtures decreases with increasing CuI-concentration. The activation energies Ea for molten AgI-CuI system were determined from the analysis of temperature dependence of σ by using the by Arrhenius type equation. Ea for molten AgI-CuI gradually increase with increasing CuIconcentration.

8. Enhanced clustering tendency of Cu-impurities with a number of oxygen vacancies in heavy carbon-loaded TiO2 - the bulk and surface morphologies

Science.gov (United States)

Zatsepin, D. A.; Boukhvalov, D. W.; Kurmaev, E. Z.; Zatsepin, A. F.; Kim, S. S.; Gavrilov, N. V.; Zhidkov, I. S.

2017-09-01

The over threshold carbon-loadings (∼50 at.%) of initial TiO2-hosts and posterior Cu-sensitization (∼7 at.%) was made using pulsed ion-implantation technique in sequential mode with 1 h vacuum-idle cycle between sequential stages of embedding. The final Cx-TiO2:Cu samples were qualified using XPS wide-scan elemental analysis, core-levels and valence band mappings. The results obtained were discussed on the theoretic background employing DFT-calculations. The combined XPS-and-DFT analysis allows to establish and prove the final formula of the synthesized samples as Cx-TiO2:[Cu+][Cu2+] for the bulk and Cx-TiO2:[Cu+][Cu0] for thin-films. It was demonstrated the in the mode of heavy carbon-loadings the remaining majority of neutral C-C bonds (sp3-type) is dominating and only a lack of embedded carbon is fabricating the O-Cdbnd O clusters. No valence base-band width altering was established after sequential carbon-copper modification of the atomic structure of initial TiO2-hosts except the dominating majority of Cu 3s states after Cu-sensitization. The crucial role of neutral carbon low-dimensional impurities as the precursors for the new phases growth was shown for Cu-sensitized Cx-TiO2 intermediate-state hosts.

9. Development of wound healing models to study TGFβ3's effect on SMA.

Science.gov (United States)

Sriram, Sriniwas; Tran, Jennifer A; Guo, Xiaoqing; Hutcheon, Audrey E K; Kazlauskas, Andrius; Zieske, James D

2017-08-01

The goal of this study was to test the efficacy of transforming growth factor beta 3 (TGFβ3) in reducing α-smooth muscle actin (SMA) expression in two models-an ex vivo organ culture and an in vitro 3D cell construct-both of which closely mimic an in vivo environment. For the ex vivo organ culture system, a central 6.0 mm corneal keratectomy was performed on freshly excised rabbit globes The corneas were then excised, segregated into groups treated with 1.0 ng/ml TGFβ1 or β3 (T1 or T3, respectively), and cultured for 2 weeks. The corneas were assessed for levels of haze and analyzed for SMA mRNA levels. For the 3D in vitro model, rabbit corneal fibroblasts (RbCFs) were cultured for 4 weeks on poly-transwell membranes in Eagle's minimum essential media (EMEM) + 10% FBS + 0.5 mM vitamin C ± 0.1 ng/ml T1 or T3. At the end of 4 weeks, the constructs were processed for analysis by indirect-immunofluorescence (IF) and RT-qPCR. The RT-qPCR data showed that SMA mRNA expression in T3 samples for both models was significantly lower (p SMA protein expression than samples treated with T1. These results show that T3 when compared to T1 decreases the expression of SMA in both ex vivo organ culture and in vitro 3D cell construct models. Understanding the mechanism of T3's action in these systems and how they differ from simple cell culture models, may potentially help in developing T3 as an anti-scarring therapy. Copyright © 2017. Published by Elsevier Ltd.

10. Brazing of Be with CuCrZr-bronze using copper-based filler metal STEMET

Directory of Open Access Journals (Sweden)

B.A. Kalin

2016-12-01

Optimizatio
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