def prepare(self, pseudocount=0.0, lenfile=None, read_length=100):
        """
        Initializes the probability of read origin according to the alignment profile

        :param pseudocount: Uniform prior for allele specificity estimation
        :return: Nothing (as it performs an in-place operations)
        """
        if self.probability.num_groups > 0:
            self.grp_conv_mat = lil_matrix((self.probability.num_loci, self.probability.num_groups))
            for i in xrange(self.probability.num_groups):
                self.grp_conv_mat[self.probability.groups[i], i] = 1.0
            self.grp_conv_mat = self.grp_conv_mat.tocsc()
            self.t2t_mat = eye(self.probability.num_loci, self.probability.num_loci)
            self.t2t_mat = self.t2t_mat.tolil()
            for tid_list in self.probability.groups:
                for ii in xrange(len(tid_list)):
                    for jj in xrange(ii):
                        i = tid_list[ii]
                        j = tid_list[jj]
                        self.t2t_mat[i, j] = 1
                        self.t2t_mat[j, i] = 1
            self.t2t_mat = self.t2t_mat.tocsc()
        if lenfile is not None:
            hid = dict(zip(self.probability.hname, np.arange(len(self.probability.hname))))
            self.target_lengths = np.zeros((self.probability.num_loci, self.probability.num_haplotypes))
            if self.probability.num_haplotypes > 1:
                with open(lenfile) as fh:
                    for curline in fh:
                        item = curline.rstrip().split("\t")
                        locus, hap = item[0].split("_")
                        self.target_lengths[self.probability.lid[locus], hid[hap]] = max(float(item[1]) - read_length + 1.0, 1.0)
            elif self.probability.num_haplotypes > 0:
                with open(lenfile) as fh:
                    for curline in fh:
                        item = curline.rstrip().split("\t")
                        self.target_lengths[self.probability.lid[item[0]], 0] = max(float(item[1]) - read_length + 1.0, 1.0)
            else:
                raise RuntimeError('There is something wrong with your emase-format alignment file.')
            self.target_lengths = self.target_lengths.transpose()
            #self.target_lengths = self.target_lengths.transpose() / read_length  # lengths in terms of read counts
            if not np.all(self.target_lengths > 0.0):
                raise RuntimeError('There exist transcripts missing length information.')
        self.probability.normalize_reads(axis=APM.Axis.READ)  # Initialize alignment probability matrix
        self.allelic_expression = self.probability.sum(axis=APM.Axis.READ)
        if self.target_lengths is not None:  # allelic_expression will be at depth-level
            self.allelic_expression = np.divide(self.allelic_expression, self.target_lengths)
        if pseudocount > 0.0:  # pseudocount is at depth-level
            orig_allelic_expression_sum = self.allelic_expression.sum()
            nzloci = np.nonzero(self.allelic_expression)[1]
            self.allelic_expression[:, nzloci] += pseudocount
            self.allelic_expression *= (orig_allelic_expression_sum / self.allelic_expression.sum())
def prepare(self, pseudocount=0.0, lenfile=None, read_length=100):
        """
        Initializes the probability of read origin according to the alignment profile

        :param pseudocount: Uniform prior for allele specificity estimation
        :return: Nothing (as it performs an in-place operations)
        """
        if self.probability.num_groups > 0:
            self.grp_conv_mat = lil_matrix((self.probability.num_loci, self.probability.num_groups))
            for i in xrange(self.probability.num_groups):
                self.grp_conv_mat[self.probability.groups[i], i] = 1.0
            self.grp_conv_mat = self.grp_conv_mat.tocsc()
            self.t2t_mat = eye(self.probability.num_loci, self.probability.num_loci)
            self.t2t_mat = self.t2t_mat.tolil()
            for tid_list in self.probability.groups:
                for ii in xrange(len(tid_list)):
                    for jj in xrange(ii):
                        i = tid_list[ii]
                        j = tid_list[jj]
                        self.t2t_mat[i, j] = 1
                        self.t2t_mat[j, i] = 1
            self.t2t_mat = self.t2t_mat.tocsc()
        if lenfile is not None:
            hid = dict(zip(self.probability.hname, np.arange(len(self.probability.hname))))
            self.target_lengths = np.zeros((self.probability.num_loci, self.probability.num_haplotypes))
            if self.probability.num_haplotypes > 1:
                with open(lenfile) as fh:
                    for curline in fh:
                        item = curline.rstrip().split("\t")
                        locus, hap = item[0].split("_")
                        self.target_lengths[self.probability.lid[locus], hid[hap]] = max(float(item[1]) - read_length + 1.0, 1.0)
            elif self.probability.num_haplotypes > 0:
                with open(lenfile) as fh:
                    for curline in fh:
                        item = curline.rstrip().split("\t")
                        self.target_lengths[self.probability.lid[item[0]], 0] = max(float(item[1]) - read_length + 1.0, 1.0)
            else:
                raise RuntimeError('There is something wrong with your emase-format alignment file.')
            self.target_lengths = self.target_lengths.transpose()
            #self.target_lengths = self.target_lengths.transpose() / read_length  # lengths in terms of read counts
            if not np.all(self.target_lengths > 0.0):
                raise RuntimeError('There exist transcripts missing length information.')
        self.probability.normalize_reads(axis=APM.Axis.READ)  # Initialize alignment probability matrix
        self.allelic_expression = self.probability.sum(axis=APM.Axis.READ)
        if self.target_lengths is not None:  # allelic_expression will be at depth-level
            self.allelic_expression = np.divide(self.allelic_expression, self.target_lengths)
        if pseudocount > 0.0:  # pseudocount is at depth-level
            orig_allelic_expression_sum = self.allelic_expression.sum()
            nzloci = np.nonzero(self.allelic_expression)[1]
            self.allelic_expression[:, nzloci] += pseudocount
            self.allelic_expression *= (orig_allelic_expression_sum / self.allelic_expression.sum())
def prepare ( self , pseudocount = 0.0 , lenfile = None , read_length = 100 ) : if self . probability . num_groups > 0 : self . grp_conv_mat = lil_matrix ( ( self . probability . num_loci , self . probability . num_groups ) ) for i in xrange ( self . probability . num_groups ) : self . grp_conv_mat [ self . probability . groups [ i ] , i ] = 1.0 self . grp_conv_mat = self . grp_conv_mat . tocsc ( ) self . t2t_mat = eye ( self . probability . num_loci , self . probability . num_loci ) self . t2t_mat = self . t2t_mat . tolil ( ) for tid_list in self . probability . groups : for ii in xrange ( len ( tid_list ) ) : for jj in xrange ( ii ) : i = tid_list [ ii ] j = tid_list [ jj ] self . t2t_mat [ i , j ] = 1 self . t2t_mat [ j , i ] = 1 self . t2t_mat = self . t2t_mat . tocsc ( ) if lenfile is not None : hid = dict ( zip ( self . probability . hname , np . arange ( len ( self . probability . hname ) ) ) ) self . target_lengths = np . zeros ( ( self . probability . num_loci , self . probability . num_haplotypes ) ) if self . probability . num_haplotypes > 1 : with open ( lenfile ) as fh : for curline in fh : item = curline . rstrip ( ) . split ( "\t" ) locus , hap = item [ 0 ] . split ( "_" ) self . target_lengths [ self . probability . lid [ locus ] , hid [ hap ] ] = max ( float ( item [ 1 ] ) - read_length + 1.0 , 1.0 ) elif self . probability . num_haplotypes > 0 : with open ( lenfile ) as fh : for curline in fh : item = curline . rstrip ( ) . split ( "\t" ) self . target_lengths [ self . probability . lid [ item [ 0 ] ] , 0 ] = max ( float ( item [ 1 ] ) - read_length + 1.0 , 1.0 ) else : raise RuntimeError ( 'There is something wrong with your emase-format alignment file.' ) self . target_lengths = self . target_lengths . transpose ( ) #self.target_lengths = self.target_lengths.transpose() / read_length  # lengths in terms of read counts if not np . all ( self . target_lengths > 0.0 ) : raise RuntimeError ( 'There exist transcripts missing length information.' ) self . probability . normalize_reads ( axis = APM . Axis . READ ) # Initialize alignment probability matrix self . allelic_expression = self . probability . sum ( axis = APM . Axis . READ ) if self . target_lengths is not None : # allelic_expression will be at depth-level self . allelic_expression = np . divide ( self . allelic_expression , self . target_lengths ) if pseudocount > 0.0 : # pseudocount is at depth-level orig_allelic_expression_sum = self . allelic_expression . sum ( ) nzloci = np . nonzero ( self . allelic_expression ) [ 1 ] self . allelic_expression [ : , nzloci ] += pseudocount self . allelic_expression *= ( orig_allelic_expression_sum / self . allelic_expression . sum ( ) )
Initializes the probability of read origin according to the alignment profile

        :param pseudocount: Uniform prior for allele specificity estimation
        :return: Nothing (as it performs an in-place operations)
Initializes the probability of read origin according to the alignment profile