def merge_pairs_after_refmapping(data, two_files, merged_out):
    """ 
    A function to merge fastq files produced by bam2fq.
    """

    ## create temp files 
    nonmerged1 = tempfile.NamedTemporaryFile(
        mode='wb',
        dir=data.dirs.edits,
        suffix="_nonmerged_R1_.fastq").name
    nonmerged2 = tempfile.NamedTemporaryFile(
        mode='wb',
        dir=data.dirs.edits,
        suffix="_nonmerged_R2_.fastq").name 

    ## get the maxn and minlen values
    minlen = str(max(32, data.paramsdict["filter_min_trim_len"]))
    try:
        maxn = sum(data.paramsdict['max_low_qual_bases'])
    except TypeError:
        maxn = data.paramsdict['max_low_qual_bases']
 
    ## set the quality scores abritrarily high and orient R2 correctly
    tmp1 = two_files[0][0]
    tmp2 = two_files[0][1]
    fastq_touchup_for_vsearch_merge(tmp1, tmp1+".tu", False)
    fastq_touchup_for_vsearch_merge(tmp2, tmp2+".tu", True)

    ## command string to call vsearch
    cmd = [ipyrad.bins.vsearch,
           "--fastq_mergepairs", tmp1+".tu",
           "--reverse", tmp2+".tu",
           "--fastqout", merged_out,
           "--fastqout_notmerged_fwd", nonmerged1,
           "--fastqout_notmerged_rev", nonmerged2,
           "--fasta_width", "0",
           "--fastq_minmergelen", minlen,
           "--fastq_maxns", str(maxn),
           "--fastq_minovlen", "10",
           "--fastq_maxdiffs", "4",
           "--label_suffix", "_m1",
           "--fastq_qmax", "1000",
           "--threads", "2",
           "--fastq_allowmergestagger"]
    
    ## run vsearch but allow kbd
    proc = sps.Popen(cmd, stderr=sps.STDOUT, stdout=sps.PIPE)
    try:
        res = proc.communicate()[0]
    except KeyboardInterrupt:
        proc.kill()

    ## cleanup tmp files if job failed or stopped    
    if proc.returncode:
        LOGGER.error("Error: %s %s", cmd, res)
        raise IPyradWarningExit("Error merge pairs:\n %s\n%s", cmd, res)

    ## record how many read pairs were merged
    with open(merged_out, 'r') as tmpf:
        nmerged = sum(1 for i in tmpf.readlines()) // 4

    ## Concat unmerged pairs with a 'nnnn' separator
    with open(merged_out, 'ab') as combout:
        ## read in paired end read files 4 lines at a time
        fr1 = open(nonmerged1, 'rb')
        quart1 = itertools.izip(*[iter(fr1)]*4)
        fr2 = open(nonmerged2, 'rb')
        quart2 = itertools.izip(*[iter(fr2)]*4)
        quarts = itertools.izip(quart1, quart2)

        ## a list to store until writing
        writing = []
        counts = 0

        ## iterate until done
        while 1:
            try:
                read1s, read2s = quarts.next()
            except StopIteration:
                break

            ## store the read
            writing.append("".join([
                read1s[0],
                read1s[1].strip() + "nnnn" + \
                read2s[1], 
                read1s[2],
                read1s[3].strip() + "nnnn" + \
                read2s[3], 
                ]))

            ## count up until time to write
            counts += 1
            if not counts % 10:
                combout.write("".join(writing))
                writing = []
        ## write the remaining
        if writing:
            combout.write("".join(writing))

        ## close handles
        fr1.close()
        fr2.close()
        combout.close()

    ## remove temp files (or do this later)
    rmfiles = [nonmerged1, nonmerged2, tmp1, tmp2, tmp1+".tu", tmp2+".tu"]
    for rmfile in rmfiles:
        if os.path.exists(rmfile):
            os.remove(rmfile)

    return nmerged
def merge_pairs_after_refmapping(data, two_files, merged_out):
    """ 
    A function to merge fastq files produced by bam2fq.
    """

    ## create temp files 
    nonmerged1 = tempfile.NamedTemporaryFile(
        mode='wb',
        dir=data.dirs.edits,
        suffix="_nonmerged_R1_.fastq").name
    nonmerged2 = tempfile.NamedTemporaryFile(
        mode='wb',
        dir=data.dirs.edits,
        suffix="_nonmerged_R2_.fastq").name 

    ## get the maxn and minlen values
    minlen = str(max(32, data.paramsdict["filter_min_trim_len"]))
    try:
        maxn = sum(data.paramsdict['max_low_qual_bases'])
    except TypeError:
        maxn = data.paramsdict['max_low_qual_bases']
 
    ## set the quality scores abritrarily high and orient R2 correctly
    tmp1 = two_files[0][0]
    tmp2 = two_files[0][1]
    fastq_touchup_for_vsearch_merge(tmp1, tmp1+".tu", False)
    fastq_touchup_for_vsearch_merge(tmp2, tmp2+".tu", True)

    ## command string to call vsearch
    cmd = [ipyrad.bins.vsearch,
           "--fastq_mergepairs", tmp1+".tu",
           "--reverse", tmp2+".tu",
           "--fastqout", merged_out,
           "--fastqout_notmerged_fwd", nonmerged1,
           "--fastqout_notmerged_rev", nonmerged2,
           "--fasta_width", "0",
           "--fastq_minmergelen", minlen,
           "--fastq_maxns", str(maxn),
           "--fastq_minovlen", "10",
           "--fastq_maxdiffs", "4",
           "--label_suffix", "_m1",
           "--fastq_qmax", "1000",
           "--threads", "2",
           "--fastq_allowmergestagger"]
    
    ## run vsearch but allow kbd
    proc = sps.Popen(cmd, stderr=sps.STDOUT, stdout=sps.PIPE)
    try:
        res = proc.communicate()[0]
    except KeyboardInterrupt:
        proc.kill()

    ## cleanup tmp files if job failed or stopped    
    if proc.returncode:
        LOGGER.error("Error: %s %s", cmd, res)
        raise IPyradWarningExit("Error merge pairs:\n %s\n%s", cmd, res)

    ## record how many read pairs were merged
    with open(merged_out, 'r') as tmpf:
        nmerged = sum(1 for i in tmpf.readlines()) // 4

    ## Concat unmerged pairs with a 'nnnn' separator
    with open(merged_out, 'ab') as combout:
        ## read in paired end read files 4 lines at a time
        fr1 = open(nonmerged1, 'rb')
        quart1 = itertools.izip(*[iter(fr1)]*4)
        fr2 = open(nonmerged2, 'rb')
        quart2 = itertools.izip(*[iter(fr2)]*4)
        quarts = itertools.izip(quart1, quart2)

        ## a list to store until writing
        writing = []
        counts = 0

        ## iterate until done
        while 1:
            try:
                read1s, read2s = quarts.next()
            except StopIteration:
                break

            ## store the read
            writing.append("".join([
                read1s[0],
                read1s[1].strip() + "nnnn" + \
                read2s[1], 
                read1s[2],
                read1s[3].strip() + "nnnn" + \
                read2s[3], 
                ]))

            ## count up until time to write
            counts += 1
            if not counts % 10:
                combout.write("".join(writing))
                writing = []
        ## write the remaining
        if writing:
            combout.write("".join(writing))

        ## close handles
        fr1.close()
        fr2.close()
        combout.close()

    ## remove temp files (or do this later)
    rmfiles = [nonmerged1, nonmerged2, tmp1, tmp2, tmp1+".tu", tmp2+".tu"]
    for rmfile in rmfiles:
        if os.path.exists(rmfile):
            os.remove(rmfile)

    return nmerged
def merge_pairs_after_refmapping ( data , two_files , merged_out ) : ## create temp files  nonmerged1 = tempfile . NamedTemporaryFile ( mode = 'wb' , dir = data . dirs . edits , suffix = "_nonmerged_R1_.fastq" ) . name nonmerged2 = tempfile . NamedTemporaryFile ( mode = 'wb' , dir = data . dirs . edits , suffix = "_nonmerged_R2_.fastq" ) . name ## get the maxn and minlen values minlen = str ( max ( 32 , data . paramsdict [ "filter_min_trim_len" ] ) ) try : maxn = sum ( data . paramsdict [ 'max_low_qual_bases' ] ) except TypeError : maxn = data . paramsdict [ 'max_low_qual_bases' ] ## set the quality scores abritrarily high and orient R2 correctly tmp1 = two_files [ 0 ] [ 0 ] tmp2 = two_files [ 0 ] [ 1 ] fastq_touchup_for_vsearch_merge ( tmp1 , tmp1 + ".tu" , False ) fastq_touchup_for_vsearch_merge ( tmp2 , tmp2 + ".tu" , True ) ## command string to call vsearch cmd = [ ipyrad . bins . vsearch , "--fastq_mergepairs" , tmp1 + ".tu" , "--reverse" , tmp2 + ".tu" , "--fastqout" , merged_out , "--fastqout_notmerged_fwd" , nonmerged1 , "--fastqout_notmerged_rev" , nonmerged2 , "--fasta_width" , "0" , "--fastq_minmergelen" , minlen , "--fastq_maxns" , str ( maxn ) , "--fastq_minovlen" , "10" , "--fastq_maxdiffs" , "4" , "--label_suffix" , "_m1" , "--fastq_qmax" , "1000" , "--threads" , "2" , "--fastq_allowmergestagger" ] ## run vsearch but allow kbd proc = sps . Popen ( cmd , stderr = sps . STDOUT , stdout = sps . PIPE ) try : res = proc . communicate ( ) [ 0 ] except KeyboardInterrupt : proc . kill ( ) ## cleanup tmp files if job failed or stopped     if proc . returncode : LOGGER . error ( "Error: %s %s" , cmd , res ) raise IPyradWarningExit ( "Error merge pairs:\n %s\n%s" , cmd , res ) ## record how many read pairs were merged with open ( merged_out , 'r' ) as tmpf : nmerged = sum ( 1 for i in tmpf . readlines ( ) ) // 4 ## Concat unmerged pairs with a 'nnnn' separator with open ( merged_out , 'ab' ) as combout : ## read in paired end read files 4 lines at a time fr1 = open ( nonmerged1 , 'rb' ) quart1 = itertools . izip ( * [ iter ( fr1 ) ] * 4 ) fr2 = open ( nonmerged2 , 'rb' ) quart2 = itertools . izip ( * [ iter ( fr2 ) ] * 4 ) quarts = itertools . izip ( quart1 , quart2 ) ## a list to store until writing writing = [ ] counts = 0 ## iterate until done while 1 : try : read1s , read2s = quarts . next ( ) except StopIteration : break ## store the read writing . append ( "" . join ( [ read1s [ 0 ] , read1s [ 1 ] . strip ( ) + "nnnn" + read2s [ 1 ] , read1s [ 2 ] , read1s [ 3 ] . strip ( ) + "nnnn" + read2s [ 3 ] , ] ) ) ## count up until time to write counts += 1 if not counts % 10 : combout . write ( "" . join ( writing ) ) writing = [ ] ## write the remaining if writing : combout . write ( "" . join ( writing ) ) ## close handles fr1 . close ( ) fr2 . close ( ) combout . close ( ) ## remove temp files (or do this later) rmfiles = [ nonmerged1 , nonmerged2 , tmp1 , tmp2 , tmp1 + ".tu" , tmp2 + ".tu" ] for rmfile in rmfiles : if os . path . exists ( rmfile ) : os . remove ( rmfile ) return nmerged
A function to merge fastq files produced by bam2fq.
A function to merge fastq files produced by bam2fq .