Abdul Hafidah was stabbed after he was hit by a car at about 17:15 BST on Thursday. He died later in hospital from a stab wound to the neck.
Police believe he had been chased by a group of men near Greenheys Lane before being hit by the blue Vauxhall Corsa on Moss Lane East and then stabbed.
A 19-year-old man is being questioned, Greater Manchester Police said.
Two other people have been arrested in connection with Mr Hafidah's death.
A 19-year-old man from Moss Side , who was arrested on suspicion of murder on Sunday after handing himself in at Longsight police station, remains in custody for questioning.
A 17-year-old boy earlier arrested on suspicion of attempted murder has been bailed pending further inquiries.
Officers are appealing for anyone with dash-cam or mobile phone footage of the incident to pass it on to them via dropbox or by calling at their local police station.
A man has been arrested on suspicion of murdering an 18-year-old man in the Moss Side area of Manchester.