Ambulance staff dealt with 2,184 calls between 19:00 on Hogmanay and 07:00 on New Year's Day.
Additional crews, call handlers and dispatchers were on duty. The busiest time was from 01:00 to 04:00, with 739 calls.
The new year total was 35 higher than the previous year. The Christmas period, from 23 to 27 December, saw a 10% increase on 2015.
Scottish Ambulance Service chief executive Pauline Howie said: "The overall level of demand for our service over the festive period has exceeded previous years and Hogmanay was one of our busiest nights of the year."
The Scottish Ambulance Service had a busier Hogmanay than last year.