Two other boys, aged 15 and 16, were assaulted and required treatment.
Police were called to reports of a large number of teenage boys involved in a disturbance at Auchinairn Gardens in Bishopbriggs at about 00:05.
The 16-year-old who was seriously assaulted was treated at Glasgow Royal Infirmary and later released. Police are seeking witnesses to the incident.
Det Sgt Jas Juttla said: "Inquiries are ongoing to establish the full circumstances surrounding this disturbance and I would appeal to any witnesses to come forward."
A 16-year-old boy has been seriously assaulted in what police said was a "large-scale disturbance" near Glasgow.