Pregnancy testing often cannot be done while an animal is loose for several reasons, including the cow or heifer is not accustomed to being in close proximity to humans, and safety to both the human handler and the animal in question. A squeeze chute is ideal, though a 6' by 3' box with a head-gate is also preferable. A "medina" gate or a gate that confines a cow so that she has no choice but to back out to escape is also good for a small number of animals that need checking.
 There are several methods available to check pregnancy, each with their strengths and weaknesses.



Rectal palpation. More commonly referred to as "preg-checking." Please see the article How to Perform Rectal Palpation in Cows and Heifers for more comprehensive information.

This is the most popular and common method used by large animal veterinarians, artificial insemination technicians and cattlemen for both beef and dairy herds, primarily because of its timeliness--it guarantees early diagnosis at 45 to 90 days post-breeding, and it allows results to be determined within seconds compared with other methods mentioned here. An experienced technician or large-animal veterinarian can perform this method and determine pregnancy with 98 percent accuracy in about 5 to 10 seconds, however technicians with less experience typically take longer to make a diagnosis, and accuracy may be lower. It's also the cheapest form of pregnancy checking compared with the other main methods like milk pregnancy testing, blood testing, and ultrasound.

Hazard of abortion is quite minimal especially when performed by experienced operators, however it is a risk with this form of pregnancy checking. Other risks associated are spread of disease from cow to cow particularly if shoulder-length gloves are not changed after preg-checking each female. If there are many females to check (well over 100 cattle, for example), that means a lot of dirty gloves to throw away. Thus, as far as risk of disease transfer is concerned, it's a risk to consider if you don't want to have to switch to a new glove after checking every cow.


The operator applies this method to recognize any changes in uterine arteries and the tone, size, and location of the uterine horns through the rectal wall. Often only one of the uterine horns (out of only two) will be either more swollen or lowered deeper into the body cavity of the female, and the uterine arteries may be more distended with extra blood pumping to the newly formed placenta connecting the fetus to the dam.



Blood test. Blood tests are used to test for pregnancy-specific proteins found in the serum of blood. Pregnancy-associated glycoproteins (PAGs) are placenta-expressed inactive aspartic proteinases often referred to as pregnancy-recognition markers. Unlike the hormone progesterone which is commonly known to be associated with pregnancy, PAGs are present only during pregnancy (and around 60 to 90 days after calving), thus are the best means to determine pregnancy status in cows. An ELISA (enzyme-linked immunosorbant assay) or RIA (radioimmunoassay) test singles out these proteinases and shows their concentration levels in both milk (see below) and blood samples. ELISA testing is more popular to use because results can be obtained faster than RIA, generally. You will not get the time frame of how far along in gestation a cow or heifer is, but you will get a yes-or-no answer on whether the female is pregnant or not, or if the female needs to be rechecked (another sample of blood is drawn from the animal in question and sent back to the lab).

Ideal for both beef and dairy herds. Blood testing is gaining popularity over palpation per rectum because of less stress and trauma on cattle, significantly reduced disease risk, and more accurate diagnosis. Blood samples are also relatively easy to get, provided a veterinarian can show you how to properly take them so that you do not need to call out a vet every time. The lab you are planning on sending your blood samples too will supply you with the tubes, the box those tubes are in and the bubble wrap they are contained in. The companies that offer these tests include BioPRYN - Biotracking LLC (Moscow, ID),PAG-IDEXX Labs (Westbrook, ME), and DG29 - Genex, CRI (Shawano, WI).
Timing is important. Cattle should not be blood tested until they are both 90 days after calving and around 30 to 50 days into gestation for optimum results.

Blood samples can be taken from either the neck or the tail. The neck offers the large jugular vein, and the tail the coccygeal vein, which is a main vessel that runs underneath the tail. Basically a sample is taken by inserting a needle straight into the vein and drawing back on the plunger until you get around 2 to 5 mL of blood. A vacuum-sealed tube with a rubber top is used to collect the blood from the syringe, simply by poking the needle into the rubber top and allowing the sample to vacate into the tube. The vacuum in the tube should be enough that you don't need to apply any pressure to the syringe to put the blood into the tube. When all samples are taken enough to fill the box supplied by the lab where you are to send them, wrap them in bubble wrap and mail your samples the very day you've taken the samples. If you cannot mail them immediately, like on a Friday or on the weekend, put them on ice or in the refrigerator until you can mail them Monday morning.
Samples must be properly labelled and accurate and precise records must also be kept in order to avoid any potential and inadvertent culling of cows that may actually be pregnant. Tag numbers (also other identification names or numbers) can be used, as long as all cows and heifers are re-tagged if they have missing tags. Remember to label the sample with which farm, which sample, and which cow it came from.



The ELISA test is generally performed as follows: PAGs are detected with anti-PAG antibody-coated plates, a detector substrate, a conjugate, and a dye with wash and incubation periods in between before results are analyzed. The dye allows the technician to judge the amount of PAG in the blood sample, since the deeper coloured the sample, the higher the concentration of PAG. Accuracy of such results are high, most samples have an accuracy of at least 90 to 99%. Sensitivity can much higher, from 97 to 100%.

Results from blood tests generally won't be sent back to the producer immediately. At the earliest, results can be expected two to three business days after the samples are sent in.





Milk Pregnancy Test. As with the blood test above, milk pregnancy test uses a PAG ELISA (Pregnancy Associated Glycoproteins Enzyme-Linked Immunosorbent Assay) test to affirm pregnancy in cows and heifers.

Recommended for herds with accurate breeding and artificial insemination (AI) records. I's a test primarily for dairy herds, however research has found equally accurate results can be had with beef breeding herds as well. Samples must be sent to a laboratory that will do this test for you. Cost per sample depends on where you are sending samples to, and through whom (i.e., for dairy producers in the US, through DHIA , or for Western Canadian dairy producers, Can-West DHI ). You can expect to pay anywhere from $3.00 to $4.50 per sample. The predominant company that can commercially perform this test for you is IDEXX Laboratories (via The IDEXX Laboratory Milk Pregnancy Test). The test itself and how it's done is exactly how PAGs in blood are tested, as described above.

Results can be obtained within 3.5 hours from the start of testing, and sent to the producer just as soon via email. In the mail, though, you should expect to get lab results back in two to three business days. Testing can be done from 28 days post-breeding until cows are dried off, even though sensitivity and specificity averages increase when cows are around 60 days into gestation. For best optimum results and to reduce chances of false positives (or even false negatives), samples should be obtained when cows are at least 90 days post-calving.





Transrectal Ultrasonography. Also known as ultrasound, this method not only determines status of pregnancy, but fetal age as well. The method utilizes a computerized unit that allows the operator to literally see what's inside the cow. There are two major parts to this ultrasound unit--the console which is the screen, and the transducer, or the probe that is inserted in the rectum of the cow. The electrically-powered console is connected to the probe via a cord so that the row of crystals in the probe can emit ultrasonic sound waves (the electricity actually causes them to change shape slightly allowing such sound waves to occur), which focuses on the tissues focused beneath the probe. The sound waves bouncing off the tissues back to the probe provides a visual image that can be seen on the console screen. The density of the tissues varies with bone, muscle, tendons, and fluids inside the body. The more dense the tissue, the more quickly sound waves return to the probe and the brighter they appear on the screen. Bone shows up bright white, whereas fluids like urine, follicles, amniotic fluid, blood vessels, etc., show up black. Because of the differences in tissue densities, the probe allows the operator to visually identify different structures, muscles and parts of the fetus.

The optimal age to perform ultrasound is 26 days post-breeding in heifers and 28-days post-breeding in cows, however skilled operators can identify pregnancies with this method once the fetal heartbeat starts, which is around 21 to 22 days post-breeding.

Ultrasound can detect fetal heartbeat and movement, which is a way to determine if the fetus is alive. Live fetuses indicate the cow or heifer is pregnant and is very likely to stay pregnant throughout the term. Dead fetuses mean that the cow or heifer will need to be rebred soon, and a hormonal shot like Estrumate® or Lutalyse® may need to be given to get the female to abort so that she can be rebred soon. This is especially true with dairy herds.


Fetal age can be determined by measuring, or estimating using what is seen on the screen, specific body parts like length of body (from the top of the head to the rump), and diameter of either the skull or the chest. Not all operators have the same preferred method for determining fetal age, some like to use more than one.

Check with your veterinarian to see what age of fetus (and hence corresponding days post-breeding) they're comfortable with identifying. Note, though, that the measurements and age relationship are the most accurate in early pregnancy, and using that means that predictions of the anticipated calving date will also be more accurate.

However, the exact time a fetus will be ready for birthing is highly variable among individuals, with frame size, fatness and other factors affecting when and if it will happen.


With fetal age also comes the possibility of sexing fetuses. Fetal sexing techniques are highly precise, yet they depend on fetal age. Best times to sex a fetus is from day 60 to 65 to when it is too large or drops over the pelvic rim.

A single scheduled ultrasound date for an entire cow herd to determine fetal sex would be very difficult because of variability in age of the fetus, even if the breeding season of said cow herd was confined to 60 days. It possibly would be best to fetal sex the entire herd when cows are at least 90 to 120 days post-breeding.




Historically transrectal ultrasound involved the technician to gain access to feel the reproductive tract of cattle via the rectum, very similar to rectal palpation described above. Recently several types of rigid probes and probe extenders have been developed to avoid risk of injury to technicians that have had to repeatedly place their arms inside of cows that have the potential to be quite uncooperative while being preg-checked. The increased safety is prevalent with optional use of a probe attached to a rigid wand, but some of the sensitivity and mobility required to perform all diagnostic techniques accurately is also lost.
Ultrasound is expensive and requires hiring a technician or veterinarian to perform this type of pregnancy diagnosis.
 Records are important to keep so that you know not only which females don't need to be culled, but also how far along they are, especially if you have left preg-checking to less than 5 months post-conception, or need to confirm which females are bred (pregnant) and which are not.
 If all other things that needed to be done have been done--such as vaccinations, re-tagging, and deworming--the animal can be released back into the herd.
 Ensure what you do with one female you try to do with all of your herd, and in a short period of time. The shorter the time between animals you have the sooner you can make the necessary decisions that need to be made. In other words, if you have to blood-test or preg-check 100 or 200 cows, make sure you do it in one day, not over an entire month. The key thing to remember is to never be in a rush to get things done: Quality takes far more precedence over quantity!
Catch and restrain the cow or heifer.
Choose your method of pregnancy detection.
Record the results.
Release the cow/heifer.
Repeat with other cows and heifers as necessary.
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