Psychological research on prejudice against homosexuals distinguishes between old-fashioned (traditional) and modern (contemporary) homonegativity, which differ with regard to their content, correlates, and consequences. The current research offers evidence for the validity of old-fashioned versus modern homonegativity distinction in the post-communist, East European context. In Studies 1 and 2 (Ns = 295 and 327, respectively) the Polish adaptations of the non-gendered and gendered Homonegativity Scale (Morrison, Parriag, & Morrison, 1999) and the Modern Homonegativity Scale (Morrison & Morrison, 2003) were developed. Furthermore, divergent (discriminant) validity of traditional and contemporary anti-homosexual prejudice was demonstrated. Old-fashioned and modern homonegativity exhibited differential relationships with social distance and support for same-sex couples' relational rights.