Human pharmaceuticals are commonly detected in the environment. Concern over these compounds in the environment center around the potential for pharmaceuticals to interfere with the endocrine system of aquatic organisms. The main focus of endocrine disruption research has centered on how estrogenic and androgenic compounds interact with-the endocrine system to elicit reproductive effects. Other classes of compounds, such as progestins, have been overlooked. Recently, studies have investigated the potential for synthetic progestins to impair reproduction and growth in aquatic organisms. The present study utilizes the OECD 210 Early-life Stage (ELS) study to investigate the impacts levonorgestrel (LNG), a synthetic progestin, on fathead minnow (FHM) survival and growth. After 28 days post-hatch, survival of larval FHM was impacted at 462 ng/L, while growth was significantly reduced at 86.9 ng/L. Further analysis was conducted by measuring specific endocrine related mRNA transcript profiles in FHM larvae following the 28 day ELS exposure to LNG. Transcripts of 3 beta-HSD, 20 beta-HSD, CYP17, AR, ER alpha, and FSH were significantly down-regulated following 28 d exposure to 16.3 ng/L LNG, while exposure to 86.9 ng/L significantly down-regulated 3 beta-HSD, 20 beta-HSD, CYP19A, and FSH. At 2392 ng/L of LNG, a significant down-regulation occurred with CYP19A and ER beta transcripts, while mPR alpha and mPR beta profiles were significantly induced. No significant changes occurred in 11 beta-HSD, CYP11A, StAR, LH beta, and VTG mRNA expression following LNG exposure. An ex vivo steroidogenesis assay was conducted with sexually mature female FHM following a 7 day exposure 100 ng/L LNG with significant reductions observed in pregnenolone, 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20-DHP), testosterone, and 11-ketotestosterone. Together these data suggest LNG can negatively impact FHM larval survival and growth, with significant alterations in endocrine related responses. (C) 2014 Elsevier B.V. All rights reserved.