The caudal part of the neural tube developed into the spinal cord at 1.5 cm CVRL (35 days) with clearly differentiated ependymal, mantle and marginal layers. From 6.4 cm CVRL (57 days) onwards, cilia were present on the ependymal cells lining the central canal. At the same stage, the mantle layer appeared at the roof and floor plate. It showed different stages of developing neurons and supporting neuroglial cells in 9.1 cm CVRL (70 days) buffalo foeti. White matter became thick and was of loose consistency at 9.1 cm CVRL.