The aim of the current study was to investigate the relationship between ambivalent sexism, specifically hostile sexism (HS) and benevolent sexism (BS), religiosity, and attitudes toward childlessness in Muslim undergraduate students. The sample consisted of 157 (79 women, 78 men) Turkish Islamic undergraduate students studying in North Cyprus, aged between 17 and 30 years-old and originating from various regions in Turkey. Participants completed measures of ambivalent sexism and attitudes toward childlessness as well as rated their level of religiosity. It was expected that due to its emphasis of traditional gender roles, benevolent sexism and high Islamic religiosity would predict negative attitudes toward childlessness in the Turkish sample. Results showed that in women, higher levels of religiosity and benevolent sexism predicted negative attitudes toward childlessness, whereas in men, benevolent sexism alone was predictive of negative attitudes toward childlessness. The results are discussed in accordance with literature on ambivalent sexism and the religion of Islam.