The Fe-57 hyperfine interactions are the useful source of information about the finest structural peculiarities in various iron-containing proteins which can be essential in the natural variety of structure and function relationship in normal biomolecules as well as in its changes in the case of molecular pathology. Mossbauer spectroscopy with a high velocity resolution as the most sensitive probe for the Fe-57 hyperfine interactions demonstrates some advances in the study of various hemoglobins, ferritins and pharmaceutical products that can bring new information for the life sciences.