The popularity of wet wipes application continues to grow, because they are an important part of skin care for millions of people around the world. Wet wipes may consist of lots of substances that can cause irritation of skin or allergy and subsequently, their composition is necessary to be identified. The aim of this study was to perform screening organic compounds in wet wipes by solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry. Screening was carried out by analysis of six samples of wet wipes. Alcohols, esters, ketones, aldehydes, acids, aromatic compounds and terpenes were identified in the samples. Among identified alcohols, methanol and phenoxyethanol that can cause allergy was identified. Propylene glycol that can be referred to a group of preservatives was found out in 2 samples. Derivatives of benzene as ethyl benzene, methylethyl benzene and butyl ethyl benzene were also observed almost in all samples. Diethyl phthalate that is not prohibited to use in hygiene products was observed in one wet wipe sample. Applied parameters of solid phase microextraction and gas chromatography-mass spectrometry can be used in future for development of quantitative methodology for particular compounds as phthalates, alkoxyalcohols, aromatic hydrocarbons and preservatives.