A high-voltage high-frequency power supply for ozone generation is presented in this paper. Ozone generation is intended to be used in air and in water disinfection. A power stage consisting of a single-phase full bridge inverter for regulating the output power, a current push-pull inverter (driver) and a control circuit are described and analyzed. This laboratory build power supply using a high voltage ferrite transformer and a PIC microcontroller was employed to energize a dielectric barrier discharge (DBD) ozone generator. The inverter working on the basis of control strategy is of simple structure and has a variation range of the working frequency in order to obtain the optimal frequency value. The experimental results concerning electrical characterization and water treatment using a cylindrical DBD ozone generator supplied by this power supply are given in the end.