Hyperlipidemia is one of the major health problem people suffering throughout the world, which is also a major risk factor for chronic heart disease that can lead to death. The current study was designed to investigate antihyperlipidemic and hepatoprotective effects of selenium modified exopolysaccharide of Lachnum sp. (SeLEP-1b) on high-fat induced mice model. Nitric acid-sodium selenite (HNO3-Na2SeO3) method was used to selenize pure LEP-1b. FT-IR and C-13 NMR results confirmed the modification of LEP-1b into -SeLEP-1b. The main structure of SeLEP-1b was similar to the original structure of native LEP-1b and -Se(O)OH group was attached to the O-6 position of C-6 in LEP-1b. Oral administration of LEP-ib and SeLEP-1b (200 mg/kg) notably reduced the serum and liver lipids, atherogenic index, and enhanced the activities of antioxidant enzymes of hyperlipidemic mice, aswell improved the histopathological status of hepatic tissues. Hence, SeLEP-1b showed better effects than LEP-1b at the same doses. SeLEP-1b demonstrated promising lipid-lowering and liver protecting activities, which may be considered as a novel compound to treat hyperlipidemia and also act as a hepatoprotective agent. (C) 2017 Published by Elsevier B.V.