The aim of this paper is to determine the effect of soil-structure interaction and time dependent material properties on behavior of concrete box-girder highway bridges. Two different finite element analyses, one stage and construction stage, have been carried out on Komurhan Bridge between Elazig and Malatya province of Turkey, over Firat River. The one stage analysis assume that structure was built in a second and material properties of structure not change under different loads and site conditions during time. However, construction stage analysis considers that construction time and time dependent material properties. The main and side spans of bridge are 135 m and 76 m, respectively. The bridge had been constructed in 3 years between 1983 and 1986 by balanced cantilever construction method. The parameters of soil-structure interaction (SSI), time dependent material properties and construction method are taken into consideration in the construction stage analysis while SSI is single parameter taking into consideration in the one stage analysis. The 3D finite element model of bridge is created the commercial program of SAP2000. Time dependent material properties are elasticity modulus, creep and shrinkage for concrete and relaxation for steel. Soft, medium, and firm soils are selected for evaluating SSI in both analyses. The results of two different finite element analyses are compared with each other. It is seen that both construction stage and SSI have a remarkable effect on the structural behavior of the bridge.