The present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Aracatuba in the state of Sao Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/92) of foals. Good quality 18S rRNA, actin, HSP70 and gp60 genes nPCR amplicons were obtained from five fecal samples. PCR amplification and sequencing of a fragment of the GP60 sporozoite surface glycoprotein gene revealed C parvum genotypes IIaAl8G3R1, lIaAl5G2R1. Interestingly, we also detected in two foals a GP60 genotype related to the human parasite C. hominis. (C) 2016 Elsevier B.V. All rights reserved.