In order to improve the quality of human being's living and working environment, different types of wood structure building, ecological architecture and green building are advocated due to their lots of advantages. This study compared three types of houses in different weather conditions on people's psychological and physiological indexes. The results showed that the temperature, electrocardiograph, heart rate and respiratory rate of people in different types of housing environment are different. Most of the physiological indexes in structural glued laminated and wood structure environment were better than those in steel and concrete structure environment, and most of the physiological indexes in structural glued laminated environment were better than those in the timber structure environment. It means that subjects of the test are easy to had comfortable and relaxed feeling in the glued laminated and timber structure environment. This provided theoretical support for the use of the product by scientific and efficient ways, and guidance for design and application of the construction of the wooden structure environment in the future.