Damage detection of a full-scale nine-story reinforced concrete building is investigated using forced vibration test (FVT). The building was the former Civil and Architecture Department of Tohoku University located in Sendai Japan. Earthquake resistance elements of this building were severely damaged in 1978 Miyagi-Ken Oki earthquake and the building was retrofitted on 2001. FVT was performed on the structure before and after retrofitting with same sensor arrangement. Using data acquired from sensors, system identification was performed using rational fraction polynomial and the damage assessment (detection and location) was done by modal assurance criterion and its derivatives. The results indicate that detected damages and their location using this method corresponds with the actual damages occurred in the structure.