Introduction: Fexofenadine (FXF) is a third-generation antihistamine drug and osthole is assumed as a natural antihistamine alternative. This paper compares results of histamine, FXF and osthole impact on HRH-1, COX-2, NF-B-K-p50, CCR1 mRNA expression. We also measured mRNA expression of IL-1 beta and CCL5/RANTES in incubated peripheral blood mononuclear cells (PBMC) to compared how histamine, FXF and osthole had influence on expression level and interacts on product secretion. Objective: The purpose was to investigate expression pattern in asthma PBMC. Methods: The cultures were treated 72 h with FXF and osthole. We measured mRNA expression of histamine HRH-1, COX-2, NF-B-K-(p)50, CCR1, IL-1 beta and CCL5/RANTES with Real-Titne PCR (RT-PCR). Results: The present study suggest that osthole may be a potential inhibitor of histamine H-1 receptor activity. We also demonstrated that cells cultured with histamine increase COX-2 mRNA expression and osthole reduce it. Conclusion:Allergy remains one of the most common chronic diseases in Europe and it is rapidly approaching epidemic proportions; with current predictions estimating that the number of allergy-afflicted will equal the healthy population by 2020. It is therefore paramount to find new pharmaceuticals which successfully combat allergic disease. (C) 2016 Elsevier GmbH. All rights reserved.