Full scale measurement on the structural dynamic characteristics and Vortex-induced Vibrations (VIV) of a long-span suspension bridge with a central span of 1650 m were conducted. Different Finite Element (FE) modeling principles for the separated twin-box girder were compared and evaluated with the field vibration test results, and the double-spine model was determined to be the best simulation model, but certain modification still needs to be made which will affect the basic modeling parameters and the dynamic response prediction values of corresponding wind tunnel tests. Based on the FE modal analysis results, small-scaled and large-scaled sectional model tests were both carried out to investigate the VIV responses, and probable Reynolds Number effects or scale effect on VIV responses were presented. Based on the observed VIV modes in the field measurement, the VIV results obtained from sectional model tests were converted into those of the three-dimensional (3D) full-scale bridge and subsequently compared with field measurement results. It is indicated that the large-scaled sectional model test can probably provide a reasonable and effective prediction on VIV response.