A new three-dimensional (3-D) Zn-based metal-organic framework with the formula of [Zn-2(L)(bpy)](CI) (1, H3L = 2,6-dihydroxypyridine-4-carboxylate, bpy = 4,4'-bipyridine), has been synthesized under hydrothermal condition. X-Ray diffraction analyse reveals that compound 1 exhibits the unique 3-D two-fold interpenetrated coordination framework with 3,4-connected fsx-3,4-C2 network topology, in which the L ligand adopts the mu(3)-bridging fashion. In addition, the experimental results of anti-inflammatory activity showed that compared with organic ligands H3L and bpy, the title compound 1 exerted rather potent activity.