Zinc is the most ubiquitous of all trace elements involved in human metabolism. It participates in all major biochemical pathways and plays multiple roles in the perpetuation of genetic material, including transcription of DNA, translation of RNA, and ultimately cellular division. We analysed 100 cases of pregnant women, from first to third trimester of pregnancy, including high risk pregnant women and normal pregnant women. The latter one served as a control group, so that we could verify the accuracy of the results. Serum blood zinc was measured directly using atomic absorption spectrometry and at the same time using total fluorescence. Patients resulting with zinc values lower than 70 mu g/d1 were marked as zinc-deficient pregnant women, whereas those with zinc values higher than 70 mu g/d1were marked as normal pregnant women. The statistical evaluation showed that there was no significant change between two methods of serum zinc determination. Without an adequate nutritional request, the person may fall in zinc deficiency condition.