One of the main challenges in water management is to determine how the current water use can condition its availability to future generations and hence its sustainability. This study proposes the use of the Water Footprint (WF) indicator to assess the environmental sustainability in water resources management at the river basin level. The current study presents the methodology developed and applies it to a case study. The WF is a relatively new indicator that measures the total volume of freshwater that is used as a production factor. Its application is ever growing in the evaluation of water use in production processes. The calculation of the WF involves water resources (blue), precipitation stored in the soil (green) and pollution (grey). It provides a comprehensive assessment of the environmental sustainability of water use in a river basin. The methodology is based upon the simulation of the anthropised water cycle, which is conducted by combining a hydrological model and a decision support system. The methodology allows the assessment of the environmental sustainability of water management at different levels, and/or ex-ante analysis of how the decisions made in water planning process affect sustainability. The sustainability study was carried out in the Segura River Basin (SRB) in South-eastern Spain. The SRB is among the most complex basins in Europe, given its special peculiarities: competition for the use, overexploitation of aquifers, pollution, alternative sources, among others. The results indicate that blue water use is not sustainable due to the generalised overexploitation of aquifers. They also reveal that surface water pollution, which is not sustainable, is mainly caused by phosphate concentrations. The assessment of future scenarios reveals that these problems will worsen if no additional measures are implemented, and therefore the water management in the SRB is environmentally unsustainable in both the short- and medium-term. (C) 2016 Elsevier B.V. All rights reserved.