Previously, we have described N-Bz-L-Glu[ NH-2-(1-benzylpiperidin-4-yl) ethyl]-O-nHex (IQM9.21, L-1) as an interesting multifunctional neuroprotective compound for the potential treatment of neurodegenerative diseases. Here, we describe new derivatives and different synthetic routes, such as chemoenzymatic and solid-phase synthesis, aiming to improve the previously described route in solution. The lipase-catalysed amidation of L-and D-Glu diesters with N-benzyl-4-(2-aminoethyl) piperidine has been studied, using Candida antarctica and Mucor miehei lipases. In all cases, the alpha-amidated compound was obtained as the main product, pointing out that regioselectivity was independent of the reacting Glu enantiomer and the nature of the lipase. An efficient solid-phase route has been used to produce new donepezil-based L-and D-Glu derivatives, resulting in good yield. At micromolar concentrations, the new compounds inhibited human cholinesterases and protected neurons against toxic insults related to Alzheimer's disease and cerebral ischemia. The CNS-permeable compounds N-Cbz-L-Glu(OEt)-[ NH-2-(1benzylpiperidin-4-yl) ethyl] (L-3) and L-1 blocked the voltage-dependent calcium channels and L-3 protected rat hippocampal slices against oxygen-glucose deprivation, becoming promising antiAlzheimer and anti-stroke lead compounds. (C) 2017 Elsevier Masson SAS. All rights reserved.