Quantitative detection of glucose is an important issue in food industry, various biotechnological processes and medication of metabolic disorders. Herein, we have reported a detection method based on Mn2+-doped-CdTe/ZnS modified nanosensor for rapid and sensitive detection of glucose levels in human biological fluids i.e. blood and urine. In this strategy Mn2+-doped CdTe/ZnS quantum dots (QDs) were designed for the direct measurement of glucose. The fluorescence (FL) of Mn2+-doped CdTe/ZnS QDs was sensitive to hydrogen peroxide (H2O2). The catalytic oxidation of glucose by glucose oxidase (GOx) produces H2O2 that extensively quenched the FL of QDs. Experimental results showed that the decrease of the QDs FL was proportional to the concentration of glucose within the range of 0.1 nM-10 mu M with the detection limit of 0.1 nM under the optimized experimental conditions. Glucose in blood and urine samples was effectively detected by this strategy. The comparison with commercialized glucose meter indicated that this proposed glucose assay system is not only simple, sensitive, but also reliable and suitable for practical application. The high sensitivity, versatility, portability, high-throughput and low cost of this glucose sensor implied its potential in point-of-care clinical diagnosis of diabetes and other fields. (C) 2017 Elsevier B.V. All rights reserved.