The updated RIDA (R) QUICK (N1402) immunochromatographic assay (R-Biopharm) for detection of norovirus was evaluated during a prospective, multicenter study using 771 stool samples from patients with gastroenteritis. Compared to real-time reverse transcriptase polymerase chain reaction (RT-rtPCR) as gold standard, the RIDA (R) QUICK had an overall sensitivity of 72.8% (91/125) and a specificity of 99.5% (640/643). Genotype analysis of the polymerase (ORF1) and capsid (ORF2) region of the genome indicated that the RIDA (R) QUICK assay could detect a broad range of genotypes including new variants (15 of 125 positive samples) which were detected by an in-house SYBR (R) Green RT-rtPCR, but not by the RIDA (R) GENE PCR PG1415 (R-Biopharm) and mostly not by the RIDA (R) GENE PCR PG1405 and the Xpert (R) Norovirus assay (Cepheid). The RIDA (R) QUICK can be used to reliably confirm norovirus in stool samples, but a negative result does not definitively exclude the presence of norovirus. (C) 2017 Elsevier Inc. All rights reserved.