Ascorbic acid plays an important role in the growth and immunity of the sea cucumber, Apostichopus japonicus, a valuable commercial species in China, South Korea, and Japan. In this study, we investigated the dietary ascorbic acid requirements of two newly discovered color morphs of A. japonicus, white and purple, and compared them with that of the common green morph. Six test diets were formulated containing 0, 50, 100, 200, 400, and 800 mg ascorbic acid equivalent per kg, as 1-ascorbyl-2-polyphosphate. The color morphs were fed the test diets for 60 days, and their growth, energy budget, and immunological performance recorded. Dietary ascorbic acid levels had a significant effect on the growth of A. japonicus (P= 200 mg/kg (P < 0.05). Superoxide dismutase (SOD) and catalase (CAT) activities, and total anti-oxidative capacity (T-AOC) were generally higher at dietary ascorbic acid levels of 100, 200, and 400 mg/kg compared with the other treatments. SOD and CAT activities and T-AOC were significantly and positively correlated with SGR, indicating that supplementary dietary ascorbic acid had a beneficial effect on growth and immunity. SGR, energy intake, and the proportion of energy allocated to growth decreased significantly in the white and purple morphs when ascorbic acid levels reached 800 mg/kg (P < 0.05); as did those of the green morph, but not to a significant level. Immune indicators decreased and MDA content increased slightly at 800 mg/kg, indicating that high levels of dietary ascorbic acid do not benefit growth or immunity. The SOD and CAT activities were higher in the purple morph than the green and white morphs, indicatingits superior immune system capacity. Optimum growth and immunity of A. japonicus were achieved at similar to 100 mg/kg of ascorbic acid in the white morph and 200 mg/kg in the green and purple morphs.