Increased knowledge of DNA-binding proteins would enhance our understanding of protein functions in cellular biological processes. To handle the explosive growth of protein sequence data, researchers have developed machine learning-based methods that quickly and accurately predict DNA-binding proteins. In recent years, the predictive accuracy of machine learning-based predictors has significantly advanced, but the predictive performance remains unsatisfactory. In this paper, we establish a novel predictor named Local-DPP, which combines the local Pse-PSSM (Pseudo Position-Specific Scoring Matrix) features with the random forest classifier. The proposed features can efficiently capture the local conservation information, together with the sequence-order information, from the evolutionary profiles (PSSMs). We evaluate and compare the Local-DPP predictor with state-of-the-art predictors on two stringent benchmark datasets (one for the jackknife test, the other for an independent test). The proposed Local-DPP significantly improved the accuracy of the existing predictors, from 77.3% to 79.2% and 76.9% to 79.0% in the jackknife and independent tests, respectively. This demonstrates the efficacy and effectiveness of Local-DPP in predicting DNA-binding proteins. (C) 2016 Elsevier Inc. All rights reserved.