Objective: We assessed the knowledge level and skills of trained ASHAs in providing home-based newborn care. Methods: 100 ASHA from two talukas of Anand district of Gujarat participated. Knowledge was assessed using a structured questionnaire while certain skills were assessed through direct observation on mannequins. Results: The mean (SD) knowledge score of the participants was 16.7(3.16) out of 34. The skills were satisfactory in 52%, 61%, 43%, and 68% of ASHA workers for temperature measurement, hand washing, weight measurement and skin-to-skin care, respectively. Large variability was observed in self reported and field performance of ASHA workers. Conclusions: Knowledge and skills of ASHA workers in this region were inadequate.