Context: Troponin T upstream open reading frame peptide (TnTuORF) may be useful as a novel biomarker in acute cardiac syndromes.Objective: The study examined the early release kinetics of TnTuORF.Materials and methods: We analyzed the time course of the release of cardiac troponins I and T and TnTuORF in patients (n=31) with hypertrophic obstructive cardiomyopathy undergoing transcoronary ablation of septal hypertrophy (TASH).Results: Fifteen minutes after TASH, the levels of both troponins increased significantly (cTnT median: 18ng/L versus 27ng/L; cTnI median: 15ng/L versus 25ng/L). TnTuORF showed no variation.Discussion: We observed a significantly greater increase in cTnI compared with cTnT.Conclusion: Our results demonstrate that troponin assays allow early detection of myocardial injury, whereas TnTuORF levels remain unchanged in this setting.