Background: N,N-diethyl-m-toluamide (DEET) is a widely used insect repellent in the United States. Objectives: To assess exposure to DEET in a representative sample of persons 6 years and older in the U.S. general population from the 2007-2010 National Health and Nutrition Examination Survey. Methods: We analyzed 5348 urine samples by using online solid-phase extraction coupled to isotope dilution high -performance liquid chromatography-tandem mass spectrometry. We used regression models to examine associations of various demographic parameters with urinary concentrations of DEET biomarkers. Results: We detected DEET in -3% of samples and at concentration ranges (>0.08 mu g/L-45.1 mu g/L) much lower than those of 3-(diethylcarbamoyl)benzoic acid (DCBA) (>0.48 mu g/L-30,400 mu g/L) and N,N-diethyl-3-hydroxymethylbenzamide (DHMB) (>0.09 mu g/L-332 g/L). DCBA was the most frequently detected metabolite (similar to 84%). Regardless of survey cycle and the person's race/ethnicity or income, adjusted geometric mean concentrations of DCBA were higher in May-Sep than in Oct-Apr. Furthermore, non-Hispanic whites in the warm season were more likely than in the colder months [adjusted odds ratio (OR) = 10.83; 95% confidence interval (CI), 3.28-35.79j and more likely than non-Hispanic blacks (OR = 3.45; 95% CI, 1.51-7.87) to have DCBA concentrations above the 95th percentile. Conclusions: The general U.S. population, including school-age children, is exposed to DEET. However, reliance on DEET as the sole urinary biomarker would likely underestimate the prevalence of exposure. Instead, oxidative metabolites of DEET are the most adequate exposure biomarkers. Differences by season of the year based on demographic variables including race/ethnicity likely reflect different lifestyle uses of DEET-containing products. Published by Elsevier Ltd