DNA molecule is the building block for most living organism. Recently, the detection of specific DNA target is a potential field not only for organism species authentication but also in disease diagnostics and gene therapy. DNA molecule has unique attribute for its molecular recognition. The two-dimensional, graphene oxide material provides excellent physio-chemical properties for utilization in the detection of specific DNA molecule. Hence, here we bring forward the structure of the DNA biomolecule. We have described the graphene and its synthesis methods, molecular binding pattern with DNA and the different detection techniques along with the potential application and use of nanomaterial. Finally, we outlined the conclusion and future perspectives in this area.