The paper presents the results of an experimental and theoretical investigation on the Pietratagliata cable-stayed bridge (Udine, Italy). Ambient vibration tests were performed in order to estimate the dynamic characteristics of the lower vibration modes of the bridge. Structural identification is carried out by means of a manual tuning procedure based on finite element models of increasingly accuracy. The analysis allows to improve the description of boundary conditions and mechanical interaction between the bridge components. Results from local dynamic testing are used to estimate the traction on the cables and to assess the integrity of the suspending system of the bridge.