Prosocial behavior is crucial for functioning societies. However, its reliable scientific assessment and the understanding of its underlying structure are still a challenge. We integrated 14 paradigms from diverse disciplines to identify reliable and method-independent subcomponents of human prosociality; 329 participants performed game theoretical paradigms and hypothetical distribution tasks commonly used in behavioral economics and completed interactive computer tasks and self-reports typically employed in psychology. Four subcomponents of prosociality were identified by exploratory factor analysis and verified by confirmatory factor analysis in an independent sample: altruistically motivated prosocial behavior, norm motivated prosocial behavior, strategically motivated prosocial behavior, and self-reported prosocial behavior. Altruistically motivated behavior was related to gender, to enhanced cognitive skills, and to reduced negative affect. Our study provides a crucial step toward an overarching framework on prosocial behavior that will benefit future research on predictors, neural underpinnings, and plasticity of human cooperation and prosociality.