BACKGROUND A high prevalence of low testosterone levels along with the conventional risk factors for diabetes mellitus type 2 have emerged in studies conducted worldwide. Thus, the purpose of the present study is to evaluate the relationship between type 2 DM and serum testosterone levels. MATERIAL AND METHODS The study was conducted in Department of Medicine, MLB. Medical College, Jhansi over the period of 2 years (2012-2014). A total of 160 diabetic patients and 80 controls were randomly selected from diabetic & medicine OPD/IPD/ICU. After taking informed consent, history and examination, free and total testosterone levels were sent after overnight fasting. Data of cases were compared to that of controls using student 't' test and p value <0.05 was considered to be significant. RESULTS The study showed that testosterone deficiency was more prevalent in diabetic subjects in comparison to controls. Free testosterone levels were more significant in terms of difference of means between study and control groups (p<0.007). CONCLUSIONS With the results based on the study carried out, we concluded that testosterone deficiency was associated with Type 2 Diabetes Mellitus. Also the study showed that free testosterone level is more affected than total testosterone level in Type 2 Diabetes patients.