Normal digestive tract microbiota plays an essential role in maintaining homeostasis and normal development of the digestive tract and metabolism, immunity and angiogenesis. Treatment with antibiotics, especially in children, is often associated with significant changes in intestinal microbiota, which can lead to intestinal disbioses. Probiotic bacteria have been used for over a century to promote digestive health. The objective of this study was to investigate the interrelations between probiotic Bifidobacterium strains isolated from infants faeces by studying their influence on the expression of antibiotic resistance of enteropathogenic strains recently isolated from digestive tract infections. This study demonstrates that probiotics associated with antibiotics increase enteropapathogens susceptibility to aminoglycosides, beta-lactams, amoxicillin plus clavulanic acid, quinolones and carbapenems.