Terms to be familiar with before you start to solve the test: 3'- and 5'-ends of DNA fragments, plasmids, restriction endonucleases, agarose gel electrophoresis, linear and circular DNA, superhelical DNA, blunt and sticky ends, DNA ligase, nuclease, [gamma-P-32] ATP, and radioactive labeling. (C) 2014 by The International Union of Biochemistry and Molecular Biology.