Magnetic separation technology is a quick and easy method for sensitive and reliable detection, separation, and purification of biomolecules from any medium. Dopamine plays a crucial role for human metabolism; hence the development of selective and robust interference free methods for dopamine (DA) detection is of great importance. In the present study, we have reported an alternative method for the detection of DA which overcomes interference by coexisting compounds. We have designed a magnetically stabilized fluidized bed system (MSFB) by using fluorescein isothiocyanate (FITC) conjugated magnetic graphene oxide sheets (magGOs) as preconcentrator and diagnostic tool. In the presence of coexisting molecules, ascorbic acid (AA) and uric acid (UA), FITC-magGOs showed selectivity to DA as 39- and 34-folds, respectively. Compared with DA, a negligible amount of AA and UA were captured by FITC- magGOs non-specifically. Maximum DA adsorption capacity of FITC-magGOs was found to be 46.21mg/g. The characteristics of the DA adsorption behavior were described by the application of the Langmuir isotherm model with monolayer adsorption more accurately. All experiments were carried out in the neutral solutions at around physiological pH.