The contamination of aquatic ecosystems with a wide range of pollutants, especially with heavy metals cause direct toxic effects and has become a matter of general concern throughout the last decades. The aim of this study was to determine the concentrations of heavy/metals (Pb, Cd, Cr, Cu, and Ni) in the water, sediments, and fish (Alburnus alburnus) of Plumbuita Lake (Bucharest, Romania), this species of fish being frequently part of the local people's diet. The results from this study provides useful information regarding concentration levels of heavy metals in the water, sediments and fish in the Plumbuita Lake, essential for the assessment of environmental quality and health of living organisms from this lake ecosystem.