Purpose: The impact of isolated malleolar fractures on the intra-articular load distribution within the ankle joint has been studied in several biomechanical cadaver studies during the last decades. Recently, computed tomography osteoabsorptiometry (CT-OAM) has been proposed as a valuable tool to assess intra-articular joint load distribution in vivo. The purpose of this retrospective matched pair analysis was to apply CT-OAM to evaluate in vivo changes of talar load distribution after lateral malleolar fractures in patients treated with open anatomic reduction and internal fixation (ORIF) compared to patients treated non-operatively. Methods: Ten matched pairs of patients with isolated lateral malleolar fractures with a maximum fracture dislocation of 3 mm and a median follow-up of 42 month were included into the study. Patients were matched for age, gender, and fracture dislocation. Range of ankle motion (ROM), the AOFAS hindfoot score and the Short Form 36 (SF-36) were evaluated. CT-OAM analysis of the injured and the uninjured contralateral ankles were performed. Results: Patients treated with ORIF showed a significant lower ROM compared to the uninjured contralateral ankle. No differences were found regarding clinical scores between patients treated by ORIF and those treated non-operatively. CT-OAM analysis showed symmetrical distribution of subchondral bone mineralization in comparison to the uninjured contralateral ankles for both groups of patients. Conclusions: The data of this study suggest that isolated lateral malleolar fractures with fracture gaps up to 3 mm are not associated with a change of the tibio-talar joint load distribution in vivo. Therefore, patients with isolated minimally displaced lateral malleolar fractures may achieve good clinical long-term outcome following non-operative treatment. (C) 2017 Elsevier Ltd. All rights reserved.