To investigate the nonlinear aerostatic stability of the Hutong cable-stayed rail-cum-road bridge with ultra-kilometer main span, a FEM bridge model is established. The tri-component wind loads and geometric nonlinearity are taken into consideration and discussed for the influence of nonlinear parameters and factors on bridge resistant capacity of aerostatic instability. The results show that the effect of initial wind attack-angle is significant for the aerostatic stability analysis of the bridge. The geometric nonlinearities of the bridge are of considerable importance in the analysis, especially the effect of cable sag. The instable mechanism of the Hutong Bridge with a steel truss girder is the spatial combination of vertical bending and torsion with large lateral bending displacement. The design wind velocity is much lower than the static instability wind velocity, and the structural aerostatic resistance capacity can meet the requirement.