We present a thorough characterization of fragmentations observed in threshold collision-induced dissociation (TCID) experiments of protonated triglycine (H(+)GGG) with Xe using a guided ion beam tandem mass spectrometer (GIBMS). Kinetic energy-dependent cross-sections for 10 ionic products are observed and analyzed to provide 0 K barriers for six primary products: [b(2)](+), [y(1) + 2H](+), [b(3)](+), CO loss, [y(2) + 2H](+), and [a(1)](+); three secondary products: [a(2)](+), [a(3)](+), and [y(2) + 2H - CO](+); and two tertiary products: high energy [y(1) + 2H](+) and [a(2) - CO](+) after accounting for multiple ion-molecule collisions, internal energy of reactant ions, unimolecular decay rates, competition between channels, and sequential dissociations. Relaxed potential energy surface scans performed at the B3LYP-D3/6-311+G(d,p) level of theory are used to identify transition states (TSs) and intermediates of the six primary and one secondary products. Geometry optimizations and single point energy calculations were performed at several levels of theory. These theoretical energies are compared with experimental energies and are found to give reasonably good agreement, in particular for the M06-2X level of theory. This good agreement between experiment and theory validates the reaction mechanisms explored computationally here and elsewhere and allows identification of the product structures formed at threshold energies. The present work presents the first measurement of absolute experimental threshold energies of important sequence ions and non-sequence ions: [y(1) + 2H](+), [b(3)](+), CO loss, [a(1)](+), and [a(3)](+), and refines those for [b(2)](+) and [y(2) + 2H](+) previously measured.