With participation in high school sports rising, the number of injuries sustained by high school athletes have also increased. This study sought to investigate the attributes of sports injuries and the common injury sites for male and female athletes to determine if certain muscles should be strengthened to prevent injuries in high school athletes. This project utilizes AnNA (anatomical network analysis) to show the relationships between the musculoskeletal system and high school sports injuries. An injury was defined as trauma to the musculoskeletal system. The study focused on the synovial joints of the human body and did not include head injuries. To do this we collected information from three data sets to develop three anatomical networks. The results showed that the articulation sites with the highest degree of muscle and bone account for the highest incidences of sports injury, most common injury site in female athletes was the ankle, but the most common injury site varied in male athletes. The results also showed that the anatomical differences between men and women account for the different injury sites. We hypothesize that strengthening the gastrocnemius muscle would help prevent injuries to knees and ankles, but further studies would need to be conducted.