A number of studies have reported on dysfunctions in steroid secretion, including altered cortisol and testosterone levels in borderline personality disorder (BDP) patients compared to healthy controls. The present study extends findings from blood and saliva studies to the cumulative measure of hair steroids. We investigated women with BPD (n = 18) and age-and education-matched healthy women (n = 17). We did not find differences between BPD patients and healthy women (p = 0.40) concerning hair cortisol levels but increased hair testosterone levels among BPD patients compared to controls (p = 0.03). These results remained when restricting the analyses to unmedicated patients. Our data indicate altered long-term testosterone but not cortisol levels in females with BPD. Future studies should address the possible impact of altered testosterone on medical illness processes including metabolic syndrome in this population. (C) 2016 Elsevier Ltd. All rights reserved.