Groundwater recharge processes, water-rock interaction and the hydraulic connectivity between aquifers of the Galilee and Eromanga Basins in central Queensland, Australia, were investigated using stable (delta H-2, delta C-18 and (87)sr/Sr-86) and radiogenic ((CI)-C-36) isotopes and dissolved methane concentrations, complemented by major ion chemistry. The central Eromanga and the upper sequence of the Galilee basins are both sub-basins of the Great Artesian Basin (GAB), and the coal seams of the Galilee Basin are currently explored for their potential as commercial coal seam gas deposits. In order to understand the potential influence of depressurisation of coal seams required to release the gas on adjacent aquifers, a detailed understanding of recharge processes and groundwater hydraulics of these basins prior to any development is required. Each of the different isotope systems were used in this study to provide different information on specific processes. For example, the assessment of delta C-13 and Sr-87/Sr-86 ratios suggested that carbonate dissolution is one of the major processes controlling the water chemistry within some aquifers. In addition, the combined assessment of delta H-2, delta O-18 and major ion chemistry indicates that transpiration is the primary process controlling the solute concentration in the GAB recharge area, whereas evaporation appears to be less significant. Groundwaters in the Galilee Basin recharge area (outside the limits of the GAB) are different to any groundwater within the GAB units. This difference is attributed to the dissolution of potassium-bearing micas, which are absent in the GAB. Groundwater age estimates based on Cl-36/Cl ratios suggest that there is a steady increase along the flow paths, and this lack of anomalous age estimates from the recharge areas to the deeper parts of the basin indicates that there is no evidence for regional inter-aquifer mixing based in isotopes only. However, dissolved methane concentrations and groundwater chemistry near faults indicates the potential mix of gas and/or water from the coal seams into the GAB groundwaters, suggesting that there may be local influence of faults as gas/water conduits. (C) 2016 Elsevier B.V. All rights reserved.