This study was conducted to validate the capability of DNA microarray technology of Olipro (TM) FoodPATH Gene Chip in detecting food-borne pathogens. Nine types of food-borne pathogen DNAs were used namely Bacillus cereus, Escherichia coli 0157:H7, Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella spp., Shigella spp. and Campylobacter spp. A total of 36 combinations of DNA templates of food-borne pathogens were used. Polymerase chain reaction (PCR) and Southern-Blotting Hybridization methods were used on the gene chip to validate its capability. The results of hibridization on gene chip were compared with results from gel electrophoresis of 2.0% (w/v). Five screenings were required to complete the 36 combinations of DNA templates of food-borne pathogen. For each screening, one gene chip was used as negative control, whereby no DNA templates of food-borne pathogen were inoculated. The results showed that all combinations of food-borne pathogen DNA templates were detected. The negative control chip did not show the presence of any DNA. This study showed that Olipro (TM) FoodPATH Gene Chip gave better results than the 2.0% (w/v) gel electrophoresis.