Perceived Vulnerability to Disease may be defined as the beliefs about personal susceptibility to the transmission of infectious diseases and the emotional discomfort associated with the potential disease transmission. To evaluate these beliefs, the Perceived Vulnerability to Disease Scale (PVD) has been used in most studies. The aim of Study 1 was to validate the PVD questionnaire to the Spanish population. The sample was made up of 744 university students from all over Spain. Confirmatory factor analysis revealed that the items on the short version of the questionnaire corresponding to an interrelated two-factor model (perceived infectability and germ aversion) showed the best fit of all the tested models. In Study 2, scales to measure antifat attitudes, disgust and negative attitudes towards AIDS were used to analyse the criterion validity of the PVD questionnaire. The sample was made up of 434 university students. It was found that germ aversion was positively related with antifat attitudes, disgust and negative attitudes towards AIDS. In light of these results, we conclude that the short version of the questionnaire has good psychometric properties and can be used by the scientific community to measure Perceived Vulnerability to Disease.