Background: Invasive fungal infection (IFI) has increased in recent years due to there being a greater number of risk factors. IFI caused by Candida is the most frequent, and although Candida albicans is the most isolated species, there is currently a decrease of C albicans and an increase of other species of the genus. Aims: To analyse the epidemiology, risk factors, and antifungal susceptibility of blood culture isolates of non-C albicans Candida species in our hospital in the last 12 years. Methods: A retrospective study was conducted on 107 patients with candidaemia admitted to our hospital. Candida isolates susceptibility to fluconazole, itraconazole, voriconazole, amphotericin B, 5-fluorocytosine, caspofungin, micafungin, and anidulafungin was determined by means of a micro dilution technique (Sensititre Yeast One; Izasa, Spain). Results: From a total of 109 strains, 59 belonged to non-C. albicans Candida species: 25 Candida parapsilosis complex, 14 Candida glabrata complex, 13 Candida tropicalis, 4 Candidci krusei, 1 Candida lipolytica, 1 Candida membranaefaciens, and 1 Candida pulcherrima. The most common risk factor in adults and children was catheter use. It was observed that 8.5% of those non-C. albicans strains were resistant to fiuconazole. Conclusions: The results of this work confirm that it is necessary in know the epidemiology of non C. albicans Candida species, the in vitro susceptibility of the species involved, and the main risk factors, especially in patients with predisposing conditions. 2016 Asociacion Espanola de Micologia. Published by Elsevier Espana, S.L.U. All rights reserved.