In this paper the experimental analysis for power harvesting from mechanical vibration on a vehicle has been studied by using QuickPack smart materials with piezoelectric effect. The finite element ANSYS method (ANSYS FEM) was applied to explore the required mechanical structure, modal and harmonic analysis, and electrical feature, i.e., output voltage, admittance. The experimental platform consists of a shocker and a lever, which simulated a periodical oscillation on vehicle vibration, for evaluating conversion efficiency from mechanical energy to electrical energy. During loading experiments of power generation, the electromechanical coupling characteristics of smart materials were investigated via a proposed testing circuit. Also, various electrical output loadings were specified within resistance of 5 similar to 3000 k Omega. Through the experiment analysis, the power harvesting test with a buck converter at the output terminal was processed to obtain the spectrum analysis of output voltage within the vibrating frequencies below 200 Hz, controlled by the electromagnetic shaker. Based on the comparison between ANSYS FEM and spectrum analysis, the optimal results of mechanical oscillating quantities have been verified by the maximum output voltage for the QuickPack NQ45N material. Hence, the optimum power harvesting of the smart material has the maximum output power of 0.18 mW at 26-Hz-vibration on a vehicle.