Introduction: The purposes of the present study was to examine the effect of intermittent exercise training on musculoskeletal and metabolic health in postmenopausal (PM) and premenopausal (PRM) women and, furthermore, to evaluate whether the adaptations can be maintained with a reduced training frequency. Methods: Eighteen PM (51 +/- 1 yr, mean +/- SEM) and 12 PRM (48 +/- 1 yr) women participated in floorball training approximately two times per week for 12 wk. In a subgroup (n = 9) of PM women (PM40), exercise training was performed for an additional 40 wk with a reduced training frequency of approximately one training session per week. Results: In PM, the body fat percentage decreased (P < 0.05) and the total lean leg mass increased (P < 0.05) during the 12-wk training period, with no changes in PRM. In both PM and PRM, lean body mass and maximal oxygen uptake ((V)over dotO(2max)) were higher, and Yo-Yo intermittent endurance test 1 (YYIET-1) performance was better (P < 0.05) after the 12-wk training period. Procollagen type 1 amino-terminal propeptide was higher (P < 0.05) in PM, and total leg bone mineral density (BMD) was higher (P < 0.05) in both PM and PRM after the 12-wk training period. In PM40, total lean leg mass, (V)over dotO(2max), YYIET-1 performance, level of procollagen type 1 amino-terminal propeptide, and total leg BMD were maintained, whereas whole-body BMD and glycated hemoglobin (HbA1c) were reduced (P < 0.05) and the expression of muscle glucose transporter type 4 was higher (P < 0.05). Conclusion: Twelve weeks of intermittent exercise training increased BMD, intermittent exercise capacity, and (V)over dotO(2max) in PM and PRM, with PM also having positive changes in body composition. Additional 40 wk of training with a reduced frequency was sufficient to preserve these physiological adaptations and also improve blood glucose regulation in PM.