This study has two objectives. On the one hand, to analyze the relationships between empathy, prosocial behavior and prosocial moral reasoning, as well as the differences between them in terms of gender. On the other hand, to analyze the predictive value of these variables on empathy, taken as a criterion variable. The sample comprised 1,557 participants (M = 13.13; DT = .86) from 12 to 15 years (47.4 % girls and 52.6 % boys). Comparison of means shows significant differences based on gender. The girls expressed higher levels of empathy, prosocial behavior, prosocial moral reasoning, in the dimensions of internalized, facing the necessity and stereotyped. The boys, meanwhile, show higher rates in physical and verbal aggressiveness and hedonistic and approval-oriented prosocial moral reasoning. Regression analysis identified as predictors of empathy in boys and girls, prosocial behavior and hedonistic reasoning. Prosocial behavior maintains direct relationships with empathy, in the case of hedonism relationships are negative. In the group of boys there are other predictors of empathy. These variables are positively associated with internalized prosocial moral reasoning and moral reasoning led to the need, and negatively with aggressiveness. Perhaps more importantly, more efficient intervention programs designed to foster specific types of prosocial behaviors.