Rainwater harvesting (RWH) provides various social, environmental, and economic benefits. It is important to first consider individual preferences and willingness to use harvested water for different purposes in order to encourage a community to use RWH and establish strategies that allow for the introduction of an alternative water supply, and additionally foster water management through sustainable practices. This paper presents the willingness of individuals to use RWH in three localities (Guanajuato, Romita, and Silao) of the state of Guanajuato, Mexico, evaluated through the acceptance of using the collected rainwater found in the results of 504 questionnaires directly applied to owners and dwelling users in urban areas of the localities studied. Other aspects related with RWH were included in the study, such as some characteristics of the population and their dwellings.