The deformational properties and fatigue of asphalt layers are important for the design and assessment of semi-rigid and flexible pavements. The Two-point Bending Test was used for determination of deformational characteristics of asphalt mixtures on Department of Construction Management in Zilina. The results are evaluated with the KATEMS assessment software. The long-term research lead to estimation of deformational characteristics and fatigue of different types of asphalt mixtures. The tests have shown a positive impact of additives (ground rubber, hydrate lime component, recycling materials,.) on the results of these mixtures.