The study on "Prenatal development of the sphenoid in the buffalo (Bubalus bubalis) was conducted at the Department of Veterinary Anatomy, College of Veterinary Science, Tirupati. A total 75 buffalo embryos and foctii starting from the 26th day to the 310th day were collected from different slaughter houses. The specimens were fixed, processed for serial paraffin sectioning and the sections were subjected to different staining methods. The prechordal part formed the basisphenoid caudally and the presphenoid cranially. The anterior end of the prechordal part joined with the nasal capsule. The prechordal part was in precartilaginous form at 40 days and cartilaginous form at 45 days. The sphenoid was developed from six ossification centers. Ossification was recognized first in the orbitosphenoid, the body of basisphenoid and the alisphenoid at 62 days.