Objective: Immune responses can change with exercise. We aimed to show the changes in cytokine levels pre-and post-exercise in patients with asthma. Methods: In this prospective control trial, data of 32 patients with asthma that was under control were classified into two groups, pre-and post-exercise. Serum IL-1 beta and monocyte IL-1 beta, IL-2, and IL-10 expressions were evaluated using enzyme-linked immunosorbent assay. The patients were advised to walk for at least 30 min for 4 days/week for 12 weeks. Results: There was no significant difference in demographic properties of the participants. Monocyte IL-1 beta levels in the pre-and post-exercise groups were 1.99 +/- 0.35 and 1.01 +/- 0.22 pg/mL, respectively (p=0.003). IL-10 levels in the pre-and post-exercise groups were 1.64 +/- 0.02 and 1.21 +/- 0.03 pg/mL, respectively (p=0.04). IL-2 levels in the pre-and post-exercise groups were 0.64 +/- 0.045 and 0.32 +/- 0.09 pg/mL, respectively (p=0.001). However, there was a significant difference in serum IL-1 beta and monocyte IL-1 beta, IL-2, and IL-10 levels between the groups (p=0.02, p=0.003, p=0.04, and p=0.001, respectively). Conclusion: Systemic inflammatory parameters that are commonly elevated in asthma may improve by exercise. The elucidation of the mechanism of immune control in patients with asthma is useful for the future treatment of asthma.