Context: Kinesio taping (KT) is a taping technique extensively used in rehabilitation of sports injuries; however, the effect of KT on delayed-onset muscle soreness is not entirely clear. Objectives: To investigate the effect of kinesio tape on the quadriceps femoris on muscle pain, flexibility, and sprint performance after squat exercise. Design: Crossover study. Setting: University research laboratory. Participants: 19 female university students (age 21.0 +/- 1.2 y, weight 53.0 +/- 4.6 kg, height 164 +/- 4 cm). Main Outcome Measures: Pressure-pain threshold for quadriceps femoris was recorded using pressure algometry. Quadriceps femoris flexibility was measured as the range of motion of knee flexion with a stainless steel goniometer. Sprint-speed measurements were conducted using photocells placed at 0 and 20 m. All participants completed both conditions (KT application and no KT application) after a 1-wk washout period. Measurements were taken at baseline and 48 h postexercise. For the KT condition, KT was applied immediately before the exercise protocol and remained on the skin for 48 h. Results: Squat exercise reduced flexibility and increased pain and sprint time compared with baseline. KT application resulted in similar sprint time and muscle pain as the no-KT condition but maintained flexibility compared with baseline. Conclusions: KT application immediately before squat exercise has no effect on muscle pain and short sprint performance but maintains muscle flexibility at 2 days of recovery.