Purpose To demonstrate the potential for a simple clinical test of hamstring muscle strength to identify susceptibility to muscle strain injury. Methods A single-case design was used; specifically, an elite-level male Australian Rules football player performed bilateral isometric maximum voluntary contractions of the hamstring muscles on a weekly basis for a period of 5 weeks preceding a right hamstring muscle strain injury. Results Minimal asymmetry (no greater than +/- 1.2% difference) was evident in the hamstring isometric maximum voluntary contractions during the first 4 weeks, but 5 days prior to injury, the right hamstring isometric maximum voluntary contraction was reduced by 10.9% compared to the left. Conclusion Measuring asymmetry in isometric maximum voluntary contractions of the hamstring muscles may be a useful clinical test to identify susceptibility to muscle strain injury.