The presence of traffic on a slender long-span bridge deck will modify the cross-section profile of the bridge, which may influence the flutter derivatives and in turn, the critical flutter wind velocity of the bridge. Studies on the influence of vehicles on the flutter derivatives and the critical flutter wind velocity of bridges are rather rare as compared to the investigations on the coupled buffeting vibration of the wind-vehicle-bridge system. A typical streamlined cross-section for long-span bridges is adopted for both experimental and analytical studies. The scaled bridge section model with vehicle models distributed on the bridge deck considering different traffic flow scenarios has been tested in the wind tunnel. The flutter derivatives of the modified bridge cross section have been identified using forced vibration method and the results suggest that the influence of vehicles on the flutter derivatives of the typical streamlined cross-section cannot be ignored. Based on the identified flutter derivatives, the influence of vehicles on the flutter stability of the bridge is investigated. The results show that the effect of vehicles on the flutter wind velocity is obvious.