Seismic performance of a self-anchored suspension bridge with three-tower were investigated. A three-dimensional nonlinear finite element model of the bridge was developed using Sap2000. Nonlinear time history analysis was conducted using Fast Nonlinear Analysis Method (FNA), while beam-column effect and nonlinear behavior of bearing and damper were taken into consideration. The analyze results showed that the middle tower was more vulnerable compared with the two side towers; there existed risk of buckling of composite beam, and the most vulnerable part lied near steel-concrete composite segment. Viscous dampers were effective to reduce seismic response of towers as well as girders.