The Differentiation of Self (DoS) is a key concept of Bowen's theory, indicating a process that begins in early infancy and progresses throughout childhood and adolescence, to reach a basic level in early adulthood. This study examined the psychometric properties of an Italian version of the Differentiation of Self Inventory-Revised (DSI-R) Skowron, Schmitt, Journal of Marital and Family Therapy 29:209-222, 2003), a multidimensional measure assessing DoS as conceptualized by Bowen. The sample comprised 671 subjects (age 19-69 year). Exploratory and confirmatory factor analyses showed that the Italian DSI-R possesses good psychometric properties. Internal consistencies were adequate. Correlations with the Dyadic Adjustment Scale, the Symptom Checklist 90-Revised, and the trait form of the State-Trait Anxiety Inventory were consistent with the theoretical relations among the constructs. The findings confirm the use of DSI-R as a psychometrically sound measure of the differentiation of self in the Italian context. Implications for future research and clinical practice are addressed.