This research addressed the effects of downscaling cokriging Land Surface Temperature (LST) on estimation of Actual Evapotranspiration (AET) from remote sensing images. Two procedures were followed. We first applied downscaling cokriging to a coarse resolution LST product of MODIS at 1000 m. With its outcome, daily AET of a medium spatial resolution (250 m) was obtained using the Surface Energy Balance System (SEBS). Second, we downScaled a coarse AET map to medium spatial resolution (250 m). For both procedures, the 250 m resolution MODIS NDVI product was used as a co-variable. Validation was carried out using Landsat 8 images, from which LST was derived from the thermal bands. The two procedures were applied to an agricultural area with a traditional irrigation network in Iran. We obtained an average LST value of 305.8 K as compared to a downscaled LST value of 307.0 K. Reference AET estimated with SEBS using Landsat 8 data was equal to 5.756 mm day(-1), as compared with a downscaled AET value of 5.571 mm day(-1). The RMSE between reference AET and downscaled AET was equal to 1.26 mm day(-1) (r = 0.49) and between reference and downscaled LST to 3.67 K (r = 0.48). The study showed that AET values obtained with the two downscaling procedures were similar to each other, but that AET showed a higher spatial variability if obtained with downscaled LST. We concluded that LST had a large effect on producing AET maps from Remote Sensing (RS) images, and that downscaling cokriging was helpful to provide daily AET maps at medium spatial resolution. (C) 2017 International Society for Photogrammetry and Remote Sensing, Inc. (ISPRS). Published by Elsevier B.V. All rights reserved.