This paper investigated field monitoring of a 1108 m suspension bridge during an assessment load test, using integrated distributed fibre-optic sensors (DFOSs). In addition to the conventional Brillouin time domain analysis system, a high spatial resolution Brillouin system using the differential pulse-width pair (DPP) technique was adopted. Temperature compensation was achieved using a Raman distributed temperature sensing system. This is the first full scale field application of DFOSs using the Brillouin time domain analysis technique in a thousand-meter-scale suspension bridge. Measured strain distributions along the whole length of the bridge were presented. The interaction between the main cables and the steel-box-girder was highlighted. The Brillouin fibre-optic monitoring systems exhibited great facility for the purposes of long distance distributed strain monitoring, with up to 0.05 m spatial resolution, and 0.01 m/point sampling interval. The performance of the Brillouin system using DPP technique was discussed. The measured data was also employed for assessing bridge design and for the assessment of structural condition. The results show that the symmetrical design assumptions were consistent with the actual bridge, and that the strain values along the whole bridge were within the safety range. This trial field study serves as an example, demonstrating the feasibility of highly dense strain and temperature measurement for large scale civil infrastructures using integrated DFOSs.