Transgenic brinjal plants (Solanum melongena cv. Co2) expressing cry1Ab gene from Bacillus thuringiensis Berliner (Bt) were evolved by Agrobacterium-mediated transformation system. In the present study, 7 T-0 lines (P-Co2IAb-5, P-Co2IAb-9, P-Co2lAb-10, P-Co2IAb-12, P-Co2IAb-16, P-Co2IAb-17 and P-Co2IAb-22) harboring cry1Ah gene were obtained. PCR and Southern blotting analyses proved the presence and integration of cry1Ab in these lines. A polyclonal rabbit anti-Cry1Ab antiserum detected the expression of a 65 kDa Cry1Ab polypeptide in all these lines. Expression of Cry1Ab protein in those transgenic lines was also confirmed by lateral flow sticks (Cry1Ab) assay. Molecular analyses in T-1 progenies harboring cry1Ab gene proved the presence and expression of the transgene of interest. The chi-square (chi(2)) tests revealed a 3:1 segregation ratio for cry1Ab gene in the lines viz., P-Co2IAb-5 and P-Co2IAb-9, indicating that the transgene was integrated at a single locus. The transgenic lines thus obtained in this study were used to investigate the potential of the Cry1Ab protein in controlling the root knot nematode, Meloidogyne incognita. Bioassay for root knot nematode resistance in T1 plants expressing Cry1Ab showed that there was a significant decrease in number of galls and eggmasses in cry1Ab-transgenic lines (P-Co2IAb-5 and P-Co2IAb-9) as compared to control. In addition, there was a significant decrease in the number of eggs per eggmass on those transgenic lines tested than the non-transgenic controls. Thus, transgenic Bt-brinjal plants could also form a part of an integrated nematode management program.