Environmental applications of graphene (GN) are limited by the occurrence of aggregation. Herein, graphene oxide (GO) was synthesized, reduced to GN by ascorbic acid, and intercalated with Cetyltrimethylammonium bromide (CTAB). GN-CTAB was characterized by Boehm's titration, N-2 adsorption/desorption, Fourier transform infrared spectroscopy, Raman spectroscopy, Fluorescence spectrophotometry, X-ray diffraction and Scanning electron microscopy. Then, GN-CTAB was used for the adsorptive removal of acid red 265 (AR265) and acid orange 7 (AO7) dyes from water both under batch and column operation. Under batch operation, the effect of pH, adsorbent dosage, initial dye concentration, contact time and temperature on dyes adsorption were assessed. Adsorption isotherms, kinetics, and thermodynamics were analyzed systematically. Regarding the fixed bed operation, the effect of both the bed height and flow rate were studied and experimental results fitted to the Thomas and BDST models. Then, the bed loss capacity along five adsorption-regeneration cycles was determined in order to further approach the practical application of GN-CTAB for wastewater treatment, namely for the removal of dyes. (C) 2017 Elsevier Inc. All rights reserved.