A new fatigue reliability assessment function which takes into account the vehicle and temperature loadings has been developed in this study. The vehicle and temperature loadings are important parameters as they can cause fatigue failure of the welds in a steel box girder. The temperature affects the traffic loading effect by changing the elastic modulus of asphalt pavement. The effect of the temperature difference has been considered based on the measured data and the finite element analysis. Linear regression equations between the equivalent stress and the temperature for different vehicle types have been developed. Using the thermal stress analysis and the rain-flow counting method, the temperature difference fatigue stress spectrum has been determined. Further, a limit equation for the fatigue reliability assessment, which takes into account both the vehicle and temperature loadings, has been developed. Finally, the effects of the temperature and the traffic growth rate on the fatigue reliability of two welding types of Nan-xi Yangtze River Suspension Bridge have been assessed. (C) 2016 Elsevier Ltd. All rights reserved.