This analyzes the effect of submersion in the formation of ochre biofilm in geotextile filters used in drainage systems. The chemical microbiological aspects involved in ochre formation and clogging of drainage systems are discussed. Clogging by ochre may be considered a major threat in the performance of filters and drainage systems. This process has been observed in the field and demonstrated in laboratory tests under well-controlled conditions. Oxygen is needed for ochre formation and is available at the water air interface of the filters. If the filters are submerged, oxygen may also be available dissolved in the water, with higher concentrations close to the surface due to the diffusion process. Column filter tests with the introduction of iron bacteria under three different filter submersion conditions were carried out. Woven geotextile filters were used in all tests. Biofilm formation on the geotextile filters were evaluated through the analysis of EDS (Energy Dispersive X-ray detector) and scanning electron microscopy. Ochre formation was verified in all tests, confirming that ochre formation can occur even under submerged conditions. The formation of ochre biofilm decreased with the depth of the geotextile filter in relation to the water surface, following the expected reduction of available oxygen below the water surface. (C) 2016 Elsevier Ltd. All rights reserved.