The activity of Bioorganic Chemistry Group (BCG) within Department of Organic Chemistry and Technology at Budapest University of Technology and Economics is related to various areas of synthetic chemistry, biotechnology and enzymology. This review gives an overview on the research activity of the group covering development of synthetic organic chemistry methods; stereoselective biotransformations with lipases, ammonia-lyases and further biocatalysts in batch and continuous-flow reactions; novel enzyme immobilization methods; and enzyme structural and mechanistic studies by experimental and computational techniques.