Study Objective: Preliminary data on the effects of prophylactic bilateral salpingectomy (PBS) show that postoperative ovarian function is preserved up to 3 months after surgery. The confirmation of PBS safety on ovarian function even many years after surgery is essential to reassure the medical community that this new strategy, recently proposed for the prevention of ovarian cancer, is at least able to avoid the risk of premature surgical menopause. We investigated whether the addition of PBS during total laparoscopic hysterectomy (TLH) causes long-term effects on ovarian function. Design: All observational study (Canadian Task Force classification II-3). Setting: Department of Obstetrics and Gynecology, "Magna Graecia" University, Catanzaro, Italy. Patients: Seventy-nine patients who underwent TLH plus salpingectomy between September 2010 and September 2012 at our institution have been recalled to be submitted to ovarian reserve evaluation in February 2015. Eight of 79 women refused to participate in this follow-up study. Interventions: The ovarian age of PBS patients has been determined through OvAge (OvAge sr., Catanzaro, Italy), a statistical model that combines antimullerian hormone, follicle-stimulating hormone, 3-dimensional antral follicle count, vascular index, flow index, and vascular flow index values. The control group consisted of a large population of 652 healthy women (with intact uterus and adnexa) previously enrolled to build the OvAge model. Comparisons between ovarian ages of PBS patients and the control group have been assessed by analysis of covariance linear statistical modeling. Measurements and Main Results: The main outcome measurement was the differences in the behavior within OvAge/age relation between PBS and control women. Descriptive statistics of those 71 enrolled PBS patients are the following: age, 49.61 +/- 2.15 years; OvAge, 49.22 +/- 2.57 years; follicle-stimulating hormone, 43.02 +/- 19.92 mU/mL; antimullerian hormone, 0.12 +/- 0.20 ng/mL; 3-dimensional antral follicle count, 1.91 +/- 1.28; vascular index, 2.80% +/- 5.32%; flow index, 19.37 +/- 5.88; and vascular flow index, 0.56 +/- 1.12. Analysis of covariance disclosed that PBS and control women do not exhibit different behaviors (p =.900) within OvAge/age relation. Conclusion: According to our model, the addition of PBS to TLH in the late reproductive years does not modify the ovarian age of treated women up to 3 to 5 years after surgery. (C) 2016 AAGL. All rights reserved.