To determine whether green teen polyphenol ( GTP) ameliorated the premature senescent and osteoporosis phenotype of Bmi-1(-/)-mice, we paired with littermates Bmi-1(+/)-between male and female mice, taken from the littermate mice. In vivo, the mice were divided into 3 groups and treated as following: 1) wild type ( WT) mice with normal diet; 2) Bmi1(-/)-( BKO) mice with normal diet; 3) Bmi1-/-mice with GTP-supplemented diet ( BKO+GTP) ( 400 mg/kg body weight/ day in the drinking water). Analysis of their phenotypic differences from the whole, X-ray, morphology, protein, histochemistry and cell biology respectively, to elucidate the roles and mechanisms of GTP in premature aging and osteoporosis phenotype of Bmi-1(-/)-mice. Our results demonstrated that Bmi-1 deficiency resulted in growth retardation, premature aging and osteoporosis. We also demonstrated that these typical aging and osteoporosis phenotypes in Bmi-1-deficient mice were largely rescued by GTP through increased proliferation and decreased apoptosis, promoted skeletal growth and development, increased osteoblastic bone formation, decreased osteoclastic bone resorption and senescence-associated molecules, down-regulating oxidative stress of multiple organs, expressing antioxidase in Bmi-1(-/)-mice. These findings implied that green tea will be a novel therapeutic way to delay aging and prevent aging-associated osteoporosis.