The need to find a better products and more efficient technique to help in removing toxic pathogens from water and help in the reduction of water pollution in the fastest, effective way and with low cost could help the worlds pollution problem. In the past, chemicals have been used for water purification and cleaning and it's still very much in use, chemical such as chlorine, ozone etc. are been used. However, research has shown that these chemical products are often given reactions with various components in natural water indicating that more than 500 different types of disinfectant byproducts obtained maybe known to be carcinogenic. Meanwhile using antibiotics as conventional method has become less effective because of the resistant strains of the antibiotics therefore the quest to design a more robust bio detection strategy is necessary. The Identification of co-precipitation method as the best method considered, this was employed in the preparation of magnetic nanoparticles using one-step reaction process. Functional group probing of the synthesized magnetic nanoparticle, as well as morphological analysis and particle size determination, were achieved by Fourier transform infrared spectroscopy (FTIR), Atomic Force Microscope (AFM) and Zeta-Sizer, respectively. If properly coated with the right and stable polymer, the use of magnetic chitosan nanoparticle can become widely known and helpful for pathogen detection with the aim of resolving the world pollution problem.