BACKGROUND Medical education is highly stressful and higher stress has been documented in medical students. Stepping entirely into a new environment, huge medical course syllabus which has to be mastered in a short period of time, continuous internal assessments, examinations, being far from family and other social and personal issues are more prone to develop negative emotional symptoms to a newly joined student. OBJECTIVE To determine the prevalence of depression, anxiety and stress among 2nd year medical students at AIMS, B G Nagar. METHODS A cross-sectional questionnaire-based study was conducted among 2nd year medical students of AIMS, B G Nagar. RESULTS Among 185 students 160 students had completed the questionnaire, the response rate was 86.46%; 118 were female students (73.75%) and 42 were male students (26.25%) and were within the age group of 19 to 21 years. In our study 41.87% of students showed depression, 68.12% of students showed anxiety and 63.12% of students showed stress of different grades (Mild, moderate, severe and very severe). The incidence of depression was almost similar in both the gender and the incidence of anxiety and stress were more among females. CONCLUSION In our study, the prevalence of anxiety and stress is more in medical students. Females are more anxious and stressful than males. Early interventions and timely monitoring should be done to address these negative emotional symptoms. Counselling of students, workshops on stress management, daily physical activities could help them to come out of these health issues.