MADS-box family transcriptional factors SHATTERPROOF1 (SHP1) and SHATTERPROOF2 (SHP2) are required for fruit dehiscence by regulating the cell specification in valve margin in Arabidopsis thaliana. BLASTN search against Brassica database in TAIR using AtSHP2 gene as a query, revealed several ESTs sharing high similarity to AtSHP2. Two highly homologous cDNAs of SHP2 were obtained from Brassica napus by PCR and RACE, and they are named as BnaA.SHP2.a and BnaC.SHP2.b, respectively. In this work, we focused on BnaA.SHP2.a. Sequence alignment and phylogenetic analysis indicated that BnaA.SHP2.a. is highly identical to AtSHP2. The southern blotting demonstrated that there are at least two copies of BnaSHP2 in B. napus genome. Semi-quantitive RT-PCR showed that BnaSHP2 was expressed in roots, floral buds, and pods, but not in stems, leaves, or seeds. BnaSHP2 was localized in the nuclei of HzAM 1 cells when fused with EGFP and expressed using HaSNPV bacmid system.