Advances in nanotechnology have enabled the production and characterization of magnetic nano-particles with nanometer-sized features that can be functionalized with material sciences for bio engineering and materials engineering etc. The iron oxides and iron oxide with other compounds are of importance in fields including biomedicine, clinical and bio-sensing applications, corrosion resistance, lubrication and magnetic properties of materials, catalyst, and geochemical processes etc. The magnetic nano-sized iron oxide particles was synthesized by the co-precipitation method from FeCl2 center dot 4H(2)O and FeCl3 center dot 6H(2)O dissolved in deionized water with vigorous stirring. It was added organic components in the solution for preventing of agglomeration of particles during synthesizing. The magnetite nanoparticles were obtained by two ways: at first by adding glycerin to the solution; second - continue stirring throughout the slow addition of fine powder of sodium oleate. It shows that the ferro-fluid with additional glycerin component and it is contained heterogeneous agglomerations of iron oxide particles with bubbled structure. While solution is added sodium oleate components, it has uniform distribution of nanoparticles in the liquid solution.