Low temperature cracking potential is a primary concern for asphalt mixtures with high RAP contents. To minimize the thermal cracking, various rejuvenators have been utilized in the past. In this study, Fourier Transform Infrared Spectroscopy (FTIR) was used to determine the level of oxidation of asphalt. To evaluate the low-temperature cracking potential of aged asphalt with and without rejuvenators, the fracture surface images were captured using Cryo-Scanning Electron Microscopy (Cryo-SEM). The Cryo-SEM images were then analyzed using the image processing technique. The rejuvenated asphalt specimens exhibited less fractured surface than the aged asphalt. Finally, the Fracture Index was computed to rank each asphalt sample in terms of low-temperature Cracking potential. Based on the test results, it can be concluded that the use of the Cryo-SEM technology combined with the image processing technique is a promising method to visually observe the fracture surface of asphalt and compute the Fracture Index to estimate the low-temperature cracking susceptibility. (C) 2016 Elsevier Ltd. All rights reserved.