This study on theprenatal development of the skull during the first trimester of pregnancy in the buffalo (Bubalus bubalis)" was conducted at Department of Veterinary Anatomy, College of Veterinary Science, Tirupati. A total of 100 buffalo embryos and foetii starting from the 26th to the 89 day were collected from different slaughter houses. The specimens were fixed and processed for serial paraffin sectioning, and the sections were subjected to different staining methods. The earliest indication of skull development was first recognized as a mass of dense mesenchyme enveloping the cranial end of the notochord at 27 days, followed by formation of the basal plate at 38 days. The ossification of the skull first appeared in the mandible, maxilla and malar bones at 45 days. Most of the chondrocranium was cartilaginous at 45 days. The chondrocranium began to ossify first at 62 days in the basioccipital and exoccipital, sphenoid and tympanic bulla. Most of the skull was ossified during the 45th to the 89th days of gestation in buffalo, during which period proper feeding of the mother by farmers is advised.