Using a large survey of Czech high school students (n=1103), aged 15-20 years, this paper explores youth prejudice to a wide range of minority groups (n=21) where family, school, and community contexts are taken into account. General Structural Equation Models are used to explore the determinants of prejudice for 21 minorities using a comparative explanatory framework where the focus is gender differences in prejudice to minorities are examined using three social theories: Social Dominance Theory (SDT), the Theory of Gendered Prejudice (TGP), and Gender Role Beliefs (GRB). This research shows that gender plays a strong role in expressing prejudiced attitudes where females express (a) less prejudiced attitudes toward the Roma, black Africans, Gays and those with mental or physical disabilities, and (b) more prejudiced attitudes towards the homeless, Vietnamese, Muslims, and lesbians. This gendered pattern is explained in terms of insights from SDT, TGP, and GRB.