ObjectivesTo investigate whether higher serum (2)-microglobulin (B2M) levels, a kidney function marker, are associated with prevalent and incident frailty in community-dwelling older women. DesignCross-sectional and longitudinal analyses of a prospective cohort. SettingPopulation-based cohort study in Tokyo, Japan. ParticipantsCommunity-dwelling women aged 75 and older with adequate data for assessing frailty status (N = 1,191) and a subset of participants without baseline frailty but with repeated frailty assessment at 2 and 4 years of follow-up. MeasurementsThe primary predictor was B2M level. Outcomes were prevalent and incident frailty during the 4-year follow-up period. Frailty was defined as presence of three of the five Fried criteria: weight loss, exhaustion, weakness, slowness, and low physical activity. Adjusted odds ratios for the main confounders were obtained using logistic regression. Discrete-time Cox proportional hazards models were used to determine the risk of developing frailty. ResultsThe study included 241 (20.2%) women with prevalent frailty at baseline and 139 (21.1%) with incident frailty during the 4-year follow-up. On multivariate analysis adjusted for multiple potential confounders, the odds of prevalent frailty were 2.5 times as great with B2M levels of 1.9 to 2.1 mg/L as with levels less than 1.6 mg/L and 2.0 times as great with B2M levels of 2.2 mg/L or more. In the unadjusted model, B2M levels of 1.9 to 2.1 mg/L were associated with a greater incidence of frailty than B2M levels of less than 1.6 mg/L (hazard ratio = 1.72, 95% confidence interval = 1.04-2.86). In the multivariate analysis adjusted for potential confounders, no significant association was noted between the highest B2M quartile and incident frailty. ConclusionHigher B2M levels were independently associated with greater frailty at baseline in older adults but only slightly associated with greater risk of incident frailty over 4 years of follow-up.