Kisspeptins and G protein coupled receptor (GPR54) play significant roles in regulating reproductive activity among seasonally reproductive animals; however, the mechanisms of KiSS-1 and GPR54 gene affecting the seasonal reproduction in striped hamster are still unknown. In this study, real-time quantitative polymerase chain reaction was employed to examine the expression profiles of KiSS-1 and GPR54 in the hypothalamus, ovaries, testes, uterus and epididymis of striped hamsters across 4 different seasons. Our results showed that, across different seasons, the KiSS-1 expression mode of male striped hamsters and the GPR54 expression mode of female striped hamsters were consistent with the seasonal photoperiod in the hypothalamus. Meanwhile, across different seasons, the expression profile of KiSS-1 in the testes and the GPR54 expression profile of male striped hamsters in the hypothalamus were consistent with the intensity of their seasonal reproductive activity. Among different tissues, the expression trend for GPR54 is consistent across 4 seasons, while that for KiSS-1 is tissue-dependent. The expression trend for GPR54 across 4 seasons is the same regardless of gender, while that for KiSS-1 is dramatically different and sex-dependent across different seasons. These results suggest that the expressions of KiSS-1 and GPR54 in the striped hamsters were regulated by complicated mechanisms, and the regulatory mechanisms in the striped hamsters are seasonal-dependent and sex-dependent. This research will provide a theoretical basis for studying how KiSS-1 and GPR54 affect seasonal reproduction and the mechanisms behind their influence.