In this article, we discuss methods of soil enzymatic analysis (pedo-enzymes) used in all specialized laboratories in recent years to determine the potential of various biochemical processes to asess and track soil fertility. Initially, general biochemical methods were used for this purpose such as the methods of Rogers (1942) for measuring phosphatase activity; Kuprevici (1951) for assesing urease activity and the method of Hofmann and Seegerer (1951) for quantifying sucrase activity; these methods relied on introducing a specific enzyme substrate in the mixture such as: beta-glycerophosphate, urea or sucrose and determining quantitative enzyme products such as phosphate ions, glucose or ammonium ions, respectively. In Biochemistry, results of enzyme tests are expressed in International Units of enzyme found in the sample of analyzed material. From the agricultural point of view, such measurement of naturally-occuring enzyme substrates are not useful, with pedo-enzymology being more focused on assessing at what level the hydrolase processes unfold, thus providing nutrients for plants. Consequently, Stefanic. (1971), Stefanic et al. (1965; 1998), Irimescu and Stefanic (1998) and Stefanic and Gheorghita (2008) recommended new methods of analysis for determining the level of total phosphatasic activity and total amidasic activity as markers of soil fertility status.