The aim of this study was to characterize and determine the inter-serovar exchange of AmpC beta-lactamase conferring plasmids isolated from humans, pigs and the swine environment. Plasmids isolated from a total of 21 antimicrobial resistant (AMR) Salmonella isolates representing human clinical cases (n = 6), pigs (n = 6) and the swine farm environment (n = 9) were characterized by replicon typing and restriction digestion, inter-serovar transferability by conjugation, and presence of AmpC beta-lactamase enzyme encoding gene bla(CMY-2) by southern hybridization. Based on replicon typing, the majority (17/21, 81%) of the plasmids belonged to the I1-I gamma Inc group and were between 70 and 103 kb. The potential for inter-serovar plasmid transfer was further confirmed by the PCR detection of AMR genes on the plasmids isolated from trans-conjugants. Plasmids from Salmonella serovars Anatum, Ouakam, Johannesburg and Typhimurium isolated from the same cohort of pigs and their environment and S. Heidelberg from a single human clinical isolate had identical plasmids based on digestion with multiple restriction enzymes (EcoRI, HindIII and PstI) and southern blotting. We demonstrated likely horizontal inter-serovar exchange of plasmid-encoding AmpC beta-lactamases resistance among MDR Salmonella serotypes isolated from pigs, swine farm environment and clinical human cases. This study provides valuable information on the role of the swine farm environment and by extension other livestock farm environments, as a potential reservoir of resistant bacterial strains that potentially transmit resistance determinants to livestock, in this case, swine, humans and possibly other hosts by horizontal exchange of plasmids. (C) 2014 Elsevier B.V. All rights reserved.