The purpose of this study was to investigate the acute effect of an active lower limb stretching routine with a sports-related training load on the hamstring eccentric length-tension relationship. 49 recreational sportsmen completed three assessment sessions, an initial familiarization session and two experimental sessions (control and stretching in random order). Immediately after the interventions (stretching or control), eccentric isokinetic peak torque, maximum force angle and total work were measured in prone position. If the alteration of the eccentric length-tension relationship could be used as a primary risk factor of hamstring muscle strains, the findings of the present study tentatively suggest that static stretching would not be able to alter the relative risk of hamstring muscles.