Rapid identification of commercially important squid species is essential to manage squid market and protect consumers' interests. In this study, two sets of loop-mediated isothermal amplification (LAMP)-based assays were developed for identification of jumbo flying squid (Dosidicus gigas). After analyzing the D. gigas mitochondrial genes reported in GenBank, the mitochondrial cytochrome c oxidase subunit I (COI) gene was selected for designing species-specific primers. When incubated at 65 A degrees C for 30 min, only D. gigas DNA can be detected by real-time fluorescence detection (RealAmp) or visual detection via SYBR GreenA (R) I staining (Visual-LAMP). The detection limits were 10 pg per reaction for purified D. gigas DNA, and 0.01 % w/w D. gigas in homogenized cephalopod mixtures. These methods can be applied to all kinds of processed squid and squid-containing products. They also have potential in on-site detection for the advantages of high specificity, high sensitivity, time saving, and ease in operation.