The Three Gorges Project is consisted of the flood control engineering, resettlement engineering, transportation engineering, electricity generation engineering, culture engineering and so forth. As a comprehensive water control project, Three Gorges Project is a unity of tangible culture and human spirits that indicating a high degree integration of naturalism and humanism and rich culture. Through a theoretical summary and research which from the perspective of culturology, Three Gorges Project culture includes flood control culture, contending scientific verification culture, migration culture, construction culture, township (city) culture, transportation culture, ecological culture, corporation culture, film and television (art) culture, national image culture, institutional culture, tourism culture, education culture and so on. The complexity of Three Gorges Project determines the dual characteristics of it which contains both positive influence and negative impact. The cultural connotation of Three Gorges Project, as a symbolization of China' s perseverance and China' s rise, greatly influences national merits and makes a surge on the relevant culture research and philosophy mediation. One day Three Gorges Project will be added to the National Cultural Heritage list and to the World Cultural Heritage list just like Dujiangyan Irrigation System and the Grand Canal. Thus, the necessity and, as time goes on, the importance of conducting a research on Three Gorges Project will be found.