from qtpy.QtWidgets import QMainWindow, QLabel,QGridLayout import napari from qtpy import uic from pathlib import Path import pandas as pd import dask.array as da # Define the main window class class FancyGUI(QMainWindow): def __init__(self, napari_viewer:napari.Viewer,codebook:pd.DataFrame,fovs,x_loc,y_loc,img_scale): # include napari_viewer as argument (it has to have this name) super().__init__() self.viewer = napari_viewer self.fovs = fovs self.x_loc = x_loc self.y_loc = y_loc self.img_scale=img_scale self.codebook = codebook self.UI_FILE = str(Path(__file__).parent / "barcode_viewer.ui") # path to .ui file uic.loadUi(self.UI_FILE, self) # load QtDesigner .ui file # self.widgetArrangement = QGridLayout() # self.widgetArrangement.addWidget(self.geneName) # self.widgetArrangement.addWidget(self.barcode) # self.setLayout(self.widgetArrangement) self.btnMakeLayer.clicked.connect(self.makeGeneLayer) self.viewer.dims.events.connect(self.updateBarcode) def updateBarcode(self): current_slice = self.viewer.dims.current_step self.lblDecodedBarcode.setText(self.codebook.iloc[current_slice[0]]['barcode']) self.lbDecodedGeneName.setText(self.codebook.iloc[current_slice[0]]['name']) def makeGeneLayer(self): current_slice = self.viewer.dims.current_step for idx,fov in enumerate(self.fovs): if idx==0: l = self.viewer.layers[f"decoded"] else: try: l = self.viewer.layers[f"decoded [{idx}]"] except KeyError: continue self.viewer.add_image( da.repeat( da.stack([l.data[current_slice[0],:,:,:,:]],axis=0), repeats = len(self.codebook), axis=0), name=f"Gene: {current_slice[0]} decoded [{idx}]", translate=(self.x_loc[idx],-self.y_loc[idx]), scale=self.img_scale, ) print("Layers added")