import json import os import numpy as np from utils import fileIO,imgproc import reports import makereports from pathlib import Path import time import re coord_file = "config.json" a_file = open(coord_file, "r") config = json.load(a_file) fov = '001' z='02' def download_azure(): if config["isRemote"]=="N" or os.path.exists(os.path.join(config['results_path'],'data')): return None os.system(config["azure_command"].format(config["raw_data_path"],os.path.join(config['results_path'],'data'))) return None def check_params(): # Check to see if there are any params that need to be found b_findz = len(config['z'])==0 b_findfov = len(config['fov'])==0 b_findir = len(config['ir'])==0 b_findwv = len(config['channel'])==0 # get all the images if config["isRemote"] == "Y": files = Path(os.path.join(config['results_path'],'data')).rglob('*.TIFF') else: files = Path(config['raw_data_path']).rglob('*.TIFF') files = list(map(str,files)) # Find all channel, ir, fov, and z from the file names re_filter = r"(.*)(\d{3})(?=nm).*(\B\d{2})\D(\B\d{3})\D(\B\d{2}\b)" param_filter = re.compile(re_filter) results = list(map(param_filter.search,files)) results =list(filter(lambda x: isinstance(x,re.Match), results)) # Get the list of parameters if they are needed # Group 0 is the full match, so each capture group is 1 indexed if b_findz: zs = list(map(lambda x: x.group(5),results)) else: zs = config['z'] if b_findir: irs = list(map(lambda x: x.group(3),results)) else: irs = config['ir'] if b_findfov: fovs = list(map(lambda x: x.group(4),results)) else: fovs = config['fov'] if b_findwv: wvs = list(map(lambda x: x.group(2),results)) else: wvs = config['channel'] if isinstance(results,list) and len(results)>0: full_raw_path = results[0].group(1) else: full_raw_path='' return sorted(list(set(zs)),key=int),sorted(list(set(fovs)),key=int),sorted(list(set(irs)),key=int),sorted(list(set(wvs)),key=int),full_raw_path def check_dirs(files): """Checks to see if the directories for the files in the list exist. If they dont, then make those directories Args: files (list[str]): the list of files whose directory paths to create. Needs to be the full, not relative paths """ if not type(files) == list: d = os.path.dirname(files) if not os.path.isdir(d): # print(files+'files' + d +'Does not exist') os.makedirs(d) else: for f in files: d = os.path.dirname(f) if d != "" and not os.path.isdir(d): # print(f+'files' + d +'Does not exist') os.makedirs(d) download_azure() zs,fovs,irs,wvs,full_raw_path = check_params() def create_image_stack(): out_file = os.path.join(config['results_path'],f'imgstack_{fov}_{z}.npy') coord_file = os.path.join(config['results_path'],f'coord_{fov}_{z}.json') check_dirs(out_file) fileIO.create_image_stack(os.path.join(full_raw_path,config['raw_image_format']),fov,z,irs,wvs,out_file,coord_file) def create_deconvolved_images(): in_file = os.path.join(config['results_path'],f'imgstack_{fov}_{z}.npy') out_file = os.path.join(config['results_path'],'deconvolved',f'deconvolved_{fov}_{z}.npy') check_dirs(out_file) imgproc._deconvolute(in_file,out_file) def create_brightness_report(): img_stack = os.path.join(config['results_path'],f'imgstack_{fov}_{z}.npy') coord_file = os.path.join(config['results_path'],f'coord_{fov}_{z}.json') out=os.path.join(config['results_path'],f'brightness_report_{fov}_{z}.pdf') check_dirs(out) makereports.brightness_worker(img_stack,coord_file,out,fov,z) def create_focus_report(): img_stack = [os.path.join(config['results_path'],f'imgstack_{fov}_{z}.npy')] coord_file = [os.path.join(config['results_path'],f'coord_{fov}_{z}.json')] out=os.path.join(config['results_path'],f'focus_report_{fov}.pdf') out_csv = os.path.join(config['results_path'],f'focus_report_{fov}.csv') check_dirs(out) makereports.focus_worker(img_stack,coord_file,out,out_csv,fov) def compile_focus_reports(): in_files = [os.path.join(config['results_path'],f'focus_report_{fov}.csv')] output = 'full_report_debug.csv' makereports.compile_focus_report(in_files,output=output,irs=irs,wvs=wvs) if __name__=='__main__': start_time = time.time() sub_start_time = time.time() create_image_stack() sub_end_time = time.time() print(f'Image Stack: {sub_end_time-sub_start_time}') sub_start_time = time.time() create_deconvolved_images() sub_end_time = time.time() print(f'Deconvolved Stack: {sub_end_time-sub_start_time}') sub_start_time = time.time() create_brightness_report() sub_end_time = time.time() print(f'Brightness Report: {sub_end_time-sub_start_time}') sub_start_time = time.time() create_focus_report() sub_end_time = time.time() print(f'Focus Report: {sub_end_time-sub_start_time}') sub_start_time = time.time() compile_focus_reports() sub_end_time = time.time() print(f'Compiled Focus Report: {sub_end_time-sub_start_time}') end_time = time.time() print(f'Total Time: {end_time-start_time}')