Title: Novobiocin Susceptibility Test: Principle, procedure and interpretations - Learn Microbiology Online
Headings: Novobiocin Susceptibility Test: Principle, procedure and interpretations
Novobiocin Susceptibility Test: Principle, procedure and interpretations
Contents
Principle of Novobiocin Test
Specimen Collection and Preparation
Procedure of Novobiocin test
Interpretation of Novobiocin test
Expected results:
Quality Control of Novobiocin test:
Limitation of Novobiocin susceptibility testing method:
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Content: It is usually performed on urine isolates (isolated from women of reproductive age group) that are coagulase-negative. Contents
1 Principle of Novobiocin Test
2 Specimen Collection and Preparation
3 Procedure of Novobiocin test
4 Quality Control of Novobiocin test: 5 Limitation of Novobiocin susceptibility testing method: Principle of Novobiocin Test
S. saprophyticus is second only to E. coli as the most frequent causative organism of uncomplicated u rinary tract infections (UTIs) in young sexually active women. So when coagulase-negative staphylococcus is isolated from a young sexually active women, a laboratory must further identify that isolate and find out is this a true pathogen ( Staphylococcus saprophyticus-novobiocin resistant) or a contaminant (i.e. Staphylococcus epidermidis-novobiocin sensitive ). Mneomonic: On the office’s STAPH retreat, there was NO StRESs. ( NO=novobiocin, S=Saprophyticus, R=Resistant, E=Epidermidis, S=Sensitive
Laboratory identification of S. saprophyticus is made on the basis of the absence of hemolysis , coagulase, and resistance to novobiocin. Novobiocin susceptibility test results are 100% sensitive and 96% speci fic. S. saprophyticus is innately resistant to the antibiotic novobiocin.