Title: Staphylococcus saprophyticus - microbewiki
Headings: Staphylococcus saprophyticus
Staphylococcus saprophyticus
Contents
Classification
Higher order taxa
Species
Description and significance
Genome structure
Cell structure and metabolism
Ecology
Pathology
Application to Biotechnology
Current Research
References

Content: 3]
In 1962, Torres Pereira first isolated a coagulase-negative Staphylococci with antigen 51 from women with acute UTI. [ 4]. This antigen was later classified as S. saprophyticus. [ 3] Laboratory tests to identify S. saprophyticus are based on its resistance to the antibiotic Novobiocin and the absence of coagulase. [ 3]
Genome structure
As of 2005, the genome of S. saprophyticus (strain ATCC 15305) has been fully sequenced by Japanese researchers. [ 1] S. saprophyticus contains a circular chromosome of 2,516,575 bp, 2,446 ORF’s, and two plasmids 38.4 and 22.9 kb in size. The genome was sequenced to better understand the pathenogenesis of the organism. Whole-genome shotgun sequencing was used, sequencing in 1-2 kb or 10 kb inserts. The inserts were then assembled using PHRED/PHRAP/CONSED programs. The gaps were filled by PCR direct sequencing, using specific primers at the ends of each gap. [