He was helped off in the 40-7 defeat by the Chiefs on Tuesday.
Captain and flanker Sam Warburton came through Tuesday's tour game as a late replacement for Ellis Jenkins, who pulled out with a tight thigh.
Back Gareth Anscombe and back-rower Ross Moriarty also withdrew from the bench with Jamie Roberts and Taulupe Faletau coming into the match squad.
Wales had already lost flanker Dan Lydiate with a shoulder injury before the tour and fellow British and Irish Lion George North departed after the opening 39-21 defeat by New Zealand because of a hamstring tear.
Prop Paul James (calf) and scrum-half Lloyd Williams (ankle) have also left the tour with prop Aaron Jarvis and 18-year-old Ospreys wing Keelan Giles called up.
Warburton was the only player in the starting XV against the Chiefs who featured in the opening Test.
Scarlets' Aled Davies and Rhys Patchell were on the bench having been flown to New Zealand as injury cover.
Ball came off the bench against New Zealand in Auckland and if he is ruled out of contention for the second Test, Luke Charteris will take over having led Wales in Hamilton.
WALES TOUR SQUAD
Backs: Gareth Davies, Rhys Webb, Aled Davies, Gareth Anscombe, Dan Biggar, Rhys Priestland, Jonathan Davies, Tyler Morgan, Jamie Roberts, Scott Williams, Hallam Amos, Matthew Morgan, Rhys Patchell, Tom James, Keelan Giles, Eli Walker, Liam Williams.
Forwards: Rob Evans, Tomas Francis, Aaron Jarvis, Gethin Jenkins, Rhodri Jones, Samson Lee, Scott Baldwin, Kristian Dacey, Ken Owens, Jake Ball, Luke Charteris, Bradley Davies, Alun Wyn Jones, Taulupe Faletau, James King, Ross Moriarty, Josh Turnbull, Ellis Jenkins, Sam Warburton (captain).

Homeowner Sue Abel said she "heard a crack on the other side of the house" at about 00:30 BST on Monday.
The stable - which was empty - had travelled about 50m (164ft) from a neighbouring field, Mrs Abel said.
She told the BBC: "I've never known the winds, in the 10 years I've been here, [to be] at that strength."
Emergency services arrived promptly but the stable was left in place on Monday because the winds were "still quite brisk", she added.
The front of the property suffered some damage, including a number of smashed windows.
Storm Katie disruption round-up
Your pictures of Storm Katie
Storm Katie - the 11th named storm of the winter - caused considerable disruption across England, south Wales and the Channel Islands on Monday.
Gusts of up to 106mph were recorded at The Needles off the Isle of Wight as the storm hit the south of England.

The 20-year-old Wales Under-19 international had a recent loan spell at Stevenage, but did not make a first-team appearance.
Crowe, who has yet to play for Ipswich, has been signed after Will Norris returned to Cambridge United.
Braintree are due to face Kidderminster at home on Tuesday after their weekend game at Torquay was postponed.

Earlier, the government announced 21 locations for enterprise zones in England in an effort to boost economic growth.
Gloucestershire's bid focused on Gloucester and Cinderford.
But Communities Minister Bob Neill said the county could still benefit from other job-creating projects.
"It's no disgrace to Gloucestershire at all, they did hard work, I know that," he said.
"And what I'd say to them is do not be discouraged.
"We are working with local enterprise partnerships and local councils beyond the enterprise zone projects, for example giving councils the ability to discount business rates to attract businesses into the area, giving the ability to set up simplified planning regimes."
Plans for 21 nationwide zones were first set out in March's Budget.
Zones have been confirmed for sites in Cheshire, Cornwall, Gosport, Norfolk, Hereford, Kent and Oxfordshire.
Other locations include Essex, Suffolk, Northampton, Leicestershire, Cambridgeshire and the Humber Estuary.
Ministers said 30,000 new jobs would be created by 2015 by giving cheaper business rates, super-fast broadband and lower levels of planning control.
Lock Jake Ball has added to Wales' injury worries before their second Test against New Zealand on Saturday.
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An entire stable was lifted by a gust of wind and dumped into the front of a house in Alderney as Storm Katie lashed the Channel Islands.
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Ipswich Town goalkeeper Michael Crowe has joined National League side Braintree Town on a one-month loan.
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Gloucestershire's failure to secure an "enterprise zone" for the county is not a reflection on local businesses, the government has said.