The Airlander 10 - part plane, part airship - was due to take off from Cardington Airfield in Bedfordshire on Sunday.
The aircraft measures 302ft (92m) long and is about 50ft (15m) longer than the biggest passenger jets.
It is not known when another attempt at a test flight will be made.
Christened the Martha Gwyn, the Â£25m aircraft can only fly in daylight for a test flight and it was feared the flight would not have been completed before dark by the time the issue was resolved, an organiser said.
It was first developed for the US government as a surveillance aircraft but the project was shelved amid defence cutbacks.
British firm Hybrid Air Vehicles (HAV) launched a campaign to return the Airlander 10 to the skies in May 2015.
The huge aircraft will be able to stay airborne for around five days during manned flights.
HAV claims it could be used for a variety of functions such as surveillance, communications, delivering aid and even passenger travel.

Operators Serco said it was a "peaceful protest" and it was talking to the women involved.
Two members of staff had earlier been suspended following allegations of abuse.
Undercover filming by Channel 4 News had shown staff referring to inmates as "animals", "beasties" and "bitches".
The facility houses up to 400 women under threat of deportation.
Speaking in Parliament on Tuesday, Home Office Minister Karen Bradley told MPs Serco "immediately suspended" one member of staff and then another after seeing the footage.
"The company has also commissioned an independent review of its culture and staffing at Yarl's Wood," she said.
Shadow Home Secretary Yvette Cooper has called for an independent inquiry into conditions at the centre.

Russian bantamweight Vladimir Nikitin will not box American Shakur Stevenson after being left bloodied by Conlan.
"The injury suffered by Vladimir is too serious," said Russian official Igor Kazikov.
Stevenson will fight Robeisy Ramirez Carrazana in the final after the Cuban beat Uzbekistan's Murodjon Akhmadaliev.
Nikitin, meanwhile, will still receive a bronze medal.
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Belfast boxer Conlan said after Tuesday's contentious decision that his Olympic dream had "been robbed".
Nikitin's face was badly bloodied after the quarter-final, which most observers felt had been conclusively won by the Irishman.
Immediately after the bout, Conlan made clear his annoyance at the verdict in an expletive-filled live TV interview with Irish broadcaster RTE.
Thursday's development may increase the the pressure on amateur boxing's world governing body, Aiba, which has faced criticism from boxers and coaches in Rio after a number of contentious judging decisions.
On Wednesday, the international body announced that it had dropped a number of officials after a review of their decisions at the Olympics.
Aiba said that, after 239 bouts in Rio, "less than a handful of the decisions were not at the level expected".
The body has admitted it is in a "transition process", but said results of bouts already contested will stand.
The maiden flight of the world's longest aircraft has been postponed at the last minute because of a "technical issue".
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About a dozen female detainees at Yarl's Wood detention centre in Bedfordshire were involved in a "small" protest on Tuesday night.
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The boxer who controversially beat Ireland's Michael Conlan in the Olympic quarter-finals has pulled out of his Olympic semi-final because of injury.