Abdul Hafidah was stabbed after he was hit by a car at about 17:15 BST on Thursday. He died later in hospital from a stab wound to the neck.
Police believe he had been chased by a group of men near Greenheys Lane before being hit by the blue Vauxhall Corsa on Moss Lane East and then stabbed.
A 19-year-old man is being questioned, Greater Manchester Police said.
Two other people have been arrested in connection with Mr Hafidah's death.
A 19-year-old man from Moss Side , who was arrested on suspicion of murder on Sunday after handing himself in at Longsight police station, remains in custody for questioning.
A 17-year-old boy earlier arrested on suspicion of attempted murder has been bailed pending further inquiries.
Officers are appealing for anyone with dash-cam or mobile phone footage of the incident to pass it on to them via dropbox or by calling at their local police station.

Striker Stamp, 37, will also be available as a player for the east Yorkshire club, who were promoted to the National League via the play-offs.
Housham was appointed last week following former manager Billy Heath's decision to switch to FC Halifax Town.
Stamp played under Housham at previous club Gainsborough Trinity,
"Darryn is a perfect match with Steve and he has that added bonus that he is still a quality striker," joint chairman Steve Forster told the club website.

It follows a damning report into the Tawel Fan ward at Glan Clwyd Hospital.
Simon Dean, interim chief executive of Betsi Cadwaladr health board, confirmed nine staff have been reported to the Nursing and Midwifery Council and three to the General Medical Council.
He will give an update on action being taken at a meeting on 11 August.
Nine members of staff have been suspended, another four redeployed and three placed on restricted duties.
A man has been arrested on suspicion of murdering an 18-year-old man in the Moss Side area of Manchester.
[NEXT_CONCEPT]
North Ferriby United have finalised their management team by appointing Darryn Stamp as assistant to new boss Steve Housham.
[NEXT_CONCEPT]
The number of staff reported to their professional bodies over care at a Denbighshire mental health ward has risen to 12.