A large tipper truck was observed delivering the tyres under cover of darkness on Wednesday night.
A council spokesperson said the bonfire was on land owned by the Housing Executive (NIHE) in Ballybeen.
"The council has been in touch with the NIHE to raise the issue of the tyres at the site and to request the matter is investigated," they said.
"The Northern Ireland Environment Agency has also been contacted by the council regarding the nature and volume of the bonfire material."
Asked about the burning of tyres on bonfires on the Radio Ulster's Nolan programme, community worker Jim Wilson there was "a very, very small minority we are talking about where we have problems".
"You're talking about businesses making money out of it, and that comes down to the PSNI to deal with it."
Meanwhile, Belfast City Council said it had received reports that tyres had been collected at a bonfire at Avoneil in the east of the city.
"We have been engaging with the local community at this site to have the tyres removed and will continue in our efforts to manage the negative impacts of the bonfire which includes the burning of tyres," a spokesperson said.
Ulster Unionist Cllr Jim Rodgers said the inclusion of tyres in bonfires was "a worrying development".
He said that councillors had met the police and asked them to be "more pro-active" regarding bonfires.

Martyn Ashton, 41, from Margam, Neath Port Talbot, had set out to raise about £7,000 for rehabilitation equipment.
Friends suggested he set up a crowd funding page rather than sell his Colnago C59 bike worth £7,000.
He described people's generosity as humbling and touching.
Mr Ashton was a professional stunt cyclist, with his videos Road Bike Party and Road Bike Party 2 gaining more than 28 million views between them on YouTube.
But he lost the use of his legs in an accident during a live show in 2013.
He said equipment for people with disabilities could prove expensive so the money would be put to good use.
"I never, ever thought it would reach £50,000," he said. "I can now think long term. It's a remarkable thing."
Lisburn and Castlereagh Council is investigating the dumping of tyres at a bonfire site in Dundonald.
[NEXT_CONCEPT]
A paralysed stunt cyclist has said he can start planning his own long-term care and rehabilitation after receiving £50,000 in donations since starting an appeal four days ago.